CA2011917A1 - Method and apparatus for in vitro reproduction and growth of cells in culture medium - Google Patents

Method and apparatus for in vitro reproduction and growth of cells in culture medium

Info

Publication number
CA2011917A1
CA2011917A1 CA2011917A CA2011917A CA2011917A1 CA 2011917 A1 CA2011917 A1 CA 2011917A1 CA 2011917 A CA2011917 A CA 2011917A CA 2011917 A CA2011917 A CA 2011917A CA 2011917 A1 CA2011917 A1 CA 2011917A1
Authority
CA
Canada
Prior art keywords
culture
cell number
sub
compartment
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2011917A
Other languages
French (fr)
Other versions
CA2011917C (en
Inventor
Van Taiariol
Robert V. Oakley
Peter Ventura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baxter International Inc
Original Assignee
Van Taiariol
Robert V. Oakley
Peter Ventura
Bio-Response, Inc.
Baxter International Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Van Taiariol, Robert V. Oakley, Peter Ventura, Bio-Response, Inc., Baxter International Inc. filed Critical Van Taiariol
Publication of CA2011917A1 publication Critical patent/CA2011917A1/en
Application granted granted Critical
Publication of CA2011917C publication Critical patent/CA2011917C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/24—Gas permeable parts

Abstract

Method and apparatus for in vitro culture of cells in culture medium to produce a viable cell mass of cell number greater than the initial cell number, wherein a cell mass of particular cell number is cultured in a first oxygen-permeable culture sub-compartment to an increased cell number, and then aseptically transferred directly from said first sub-compartment to a next serial culture sub-compartment providing additional medium and/or culture space for further growth and reproduction to produce a viable cell mass of still higher cell number. Continued transfers are made, if necessary, to successive serial culture sub-compartments until a viable cell mass of desired cell number is attained, particularly one useful for seeding of a production-scale culture system.
CA002011917A 1989-03-10 1990-03-09 Method and apparatus for in vitro reproduction and growth of cells in culture medium Expired - Fee Related CA2011917C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US321,765 1989-03-10
US07/321,765 US5017490A (en) 1989-03-10 1989-03-10 Method for in vitro reproduction and growth of cells in culture medium

Publications (2)

Publication Number Publication Date
CA2011917A1 true CA2011917A1 (en) 1990-09-10
CA2011917C CA2011917C (en) 2000-01-25

Family

ID=23251935

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002011917A Expired - Fee Related CA2011917C (en) 1989-03-10 1990-03-09 Method and apparatus for in vitro reproduction and growth of cells in culture medium

Country Status (9)

Country Link
US (1) US5017490A (en)
EP (1) EP0418354B1 (en)
JP (1) JP2981684B2 (en)
AT (1) ATE140956T1 (en)
CA (1) CA2011917C (en)
DE (1) DE69027962T2 (en)
DK (1) DK0418354T3 (en)
ES (1) ES2092506T3 (en)
WO (1) WO1990010690A1 (en)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4027126C1 (en) * 1990-08-28 1991-12-12 Eberhard Prof. Dr. 2000 Hamburg De Bock
EP0507933B1 (en) * 1990-10-31 1996-05-22 Baxter International Inc. Close vascularization implant material
US5330915A (en) * 1991-10-18 1994-07-19 Endotronics, Inc. Pressure control system for a bioreactor
IL102189A (en) * 1992-06-12 1995-07-31 Univ Ben Gurion Microorganism growth apparatus
DE4303936C1 (en) * 1993-02-10 1994-08-18 Gore W L & Ass Gmbh Device for removing gaseous substances from a gas stream
DK0663817T3 (en) 1993-08-10 2000-03-13 Gore & Ass cell encapsulation
JPH0780041A (en) * 1993-09-20 1995-03-28 Terumo Corp Cell preserving bag system
US6297046B1 (en) 1994-10-28 2001-10-02 Baxter International Inc. Multilayer gas-permeable container for the culture of adherent and non-adherent cells
US5935847A (en) 1994-10-28 1999-08-10 Baxter International Inc. Multilayer gas-permeable container for the culture of adherent and non-adherent cells
US5728581A (en) * 1995-06-07 1998-03-17 Systemix, Inc. Method of expanding hematopoietic stem cells, reagents and bioreactors for use therein
US6024220A (en) 1995-06-07 2000-02-15 Baxter International Inc. Encapsulated seam for multilayer materials
US6391404B1 (en) 1995-06-07 2002-05-21 Baxter International Inc. Coextruded multilayer film materials and containers made therefrom
AU6854696A (en) * 1995-09-22 1997-04-09 Gore Hybrid Technologies, Inc. Improved cell encapsulation device
US5964261A (en) 1996-05-29 1999-10-12 Baxter International Inc. Implantation assembly
DE19707497A1 (en) * 1996-08-16 1998-02-19 Boehringer Mannheim Gmbh Reaction bag device for carrying out multistage cultivation / separation processes and / or reactions
CN1185339C (en) 1997-12-24 2005-01-19 杜菲尔国际开发有限公司 Preparation of cells for production of biologicals
US6183460B1 (en) 1998-01-22 2001-02-06 Baxter International Inc. Multi-use solution container having flaps
US7326564B2 (en) 2001-02-20 2008-02-05 St. Jude Medical, Inc. Flow system for medical device evaluation and production
WO2005059091A1 (en) * 2003-12-18 2005-06-30 Hitachi Medical Corporation Device for cell culture
WO2005083052A1 (en) * 2004-02-23 2005-09-09 De Crecy Eudes Francois Marie Continuous culture apparatus with mobile vessel, allowing selection of filter cell variants
EP1739165A1 (en) * 2005-06-29 2007-01-03 Cellution Biotech B.V. Method and apparatus for cultivating cells utilizing wave motion
US7745209B2 (en) 2005-07-26 2010-06-29 Corning Incorporated Multilayered cell culture apparatus
US20080131959A1 (en) * 2006-11-15 2008-06-05 Millipore Corporation Bioreactor construction
US20080131960A1 (en) * 2006-11-15 2008-06-05 Millipore Corporation Self standing bioreactor construction
CA2671967A1 (en) 2006-12-07 2008-06-19 Wilson Wolf Manufacturing Corporation Highly efficient devices and methods for culturing cells
CN101541946B (en) * 2007-01-22 2013-08-14 伊恩·梅尔康·莱特 Variable volume bioreactor
JP5023795B2 (en) * 2007-04-27 2012-09-12 東洋製罐株式会社 Cell culture method, cell culture system, and medium adjustment device
CN101668843A (en) 2007-04-27 2010-03-10 东洋制罐株式会社 Cell culture apparatus, cell culture system and cell culture method
US8114664B2 (en) * 2007-07-12 2012-02-14 Worcester Polytechnic Institute Scalable wall bioreactor for culture of plant and animal tissues
US8367400B2 (en) * 2008-03-21 2013-02-05 Corning Incorporated Culture vessel allowing large in situ scale up
JP5309657B2 (en) * 2008-04-01 2013-10-09 東洋製罐グループホールディングス株式会社 Culture vessel
JP5309658B2 (en) * 2008-04-01 2013-10-09 東洋製罐グループホールディングス株式会社 Culture method and culture apparatus
WO2009123173A1 (en) * 2008-04-01 2009-10-08 東洋製罐株式会社 Culture container, culture method and culture apparatus
US20100055764A1 (en) * 2008-08-27 2010-03-04 Martin Gregory R Cell culture apparatus and method
US20100209966A1 (en) * 2009-02-18 2010-08-19 Biolex Therapeutics, Inc. Aseptic bioreactor system for processing biological materials
EP2424982A4 (en) * 2009-04-29 2013-01-09 Crecy Eudes De Adapting microorganisms for agricultural products
JP5740841B2 (en) * 2010-05-19 2015-07-01 東洋製罐グループホールディングス株式会社 CELL CULTURE METHOD, CELL CULTURE DEVICE, AND CELL CULTURE DEVICE CONTROL METHOD
DE102014202261A1 (en) * 2014-02-07 2015-08-27 Eurozyto Gmbh Container and set for providing parenteral nutrition
JP6405690B2 (en) * 2014-05-09 2018-10-17 東洋製罐グループホールディングス株式会社 Multi-chamber culture container and cell culture method
CN106459873A (en) * 2014-05-28 2017-02-22 通用电气健康护理生物科学股份公司 Bag assembly for cultivation of cells
US10731118B2 (en) 2014-05-28 2020-08-04 Ge Healthcare Bio-Sciences Corp. Bag assembly for cultivation of cells
CN107208030B (en) * 2015-01-30 2020-12-18 东洋制罐集团控股株式会社 Cell culture method and cell culture apparatus
CN107208028A (en) * 2015-02-05 2017-09-26 通用电气公司 Bioreactor system for cell culture
CA2979789A1 (en) * 2015-03-20 2016-09-29 Sekisui Chemical Co., Ltd. Microorganism culture method and culture apparatus
US11566218B2 (en) 2016-07-05 2023-01-31 Global Life Sciences Solutions Usa Llc Apparatus and methods for adjustable volume cell culture
CN111801412A (en) * 2018-03-02 2020-10-20 赛默电子Led有限公司 Disposable centrifugation containers for separating biological suspensions and methods of use
EP3650528A1 (en) * 2018-11-08 2020-05-13 Aglaris Ltd Cell culture system and method
EP4352197A1 (en) * 2021-06-07 2024-04-17 SOTIO Biotech Inc. Cell culture vessel for use in manufacturing cell products
GB2617629B (en) * 2022-06-29 2024-04-10 Hoxton Farms Ltd System for cell culture

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1792450A (en) * 1927-10-24 1931-02-10 Stich Eugen Process and appliance for fermenting sacchariferous liquids
US3102082A (en) * 1961-07-17 1963-08-27 John H Brewer Apparatus and method for culturing micro-organisms
US3257072A (en) * 1963-01-07 1966-06-21 Cryogenic Eng Co Whole blood storage structure
US3814395A (en) * 1964-02-27 1974-06-04 Kaelin J R Aeration plant for clarifying sewage and waste effluents
US3911918A (en) * 1972-04-13 1975-10-14 Ralph D Turner Blood collection, storage and administering bag
US3946780A (en) * 1973-01-04 1976-03-30 Sellers John C Fermentation container
US4132594A (en) * 1976-06-28 1979-01-02 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Gas diffusion liquid storage bag and method of use for storing blood
US4059486A (en) * 1976-11-03 1977-11-22 Monsanto Company Cell culture process
IT1122151B (en) * 1978-07-26 1986-04-23 Biotest Serum Institut Gmbh SYSTEM OF BAGS FOR THE BLOOD GROWING
CS231615B1 (en) * 1981-12-29 1984-12-14 Vladislav Vlcek Method of cultivation of cells of higher organism and device to perform the method
US4507114A (en) * 1983-10-21 1985-03-26 Baxter Travenol Laboratories, Inc. Multiple chamber container having leak detection compartment
US4632244A (en) * 1986-02-19 1986-12-30 Boris Landau Multiple chamber flexible container
US4829002A (en) * 1986-05-12 1989-05-09 Baxter International Inc. System for metering nutrient media to cell culture containers and method
US4734373A (en) * 1986-06-24 1988-03-29 Bartal Arie H Apparatus for enhancing cell growth, preservation and transport
US4717668A (en) * 1986-12-12 1988-01-05 Baxter Travenol Laboratories, Inc. Plastic roller bottle for suspension cultures

Also Published As

Publication number Publication date
EP0418354B1 (en) 1996-07-31
DK0418354T3 (en) 1996-08-26
EP0418354A4 (en) 1991-12-27
DE69027962D1 (en) 1996-09-05
DE69027962T2 (en) 1997-01-02
JP2981684B2 (en) 1999-11-22
CA2011917C (en) 2000-01-25
WO1990010690A1 (en) 1990-09-20
ATE140956T1 (en) 1996-08-15
EP0418354A1 (en) 1991-03-27
JPH03505164A (en) 1991-11-14
US5017490A (en) 1991-05-21
ES2092506T3 (en) 1996-12-01

Similar Documents

Publication Publication Date Title
CA2011917A1 (en) Method and apparatus for in vitro reproduction and growth of cells in culture medium
CA2122141A1 (en) Methods for selectively expanding stem cells
CA2209211A1 (en) Production of a contractile smooth muscle
EP0764719A3 (en) Methods for animal cell culture
AU641488B2 (en) Human hematopoietic stem cell
EP0232850A3 (en) Fermentation process
CA2115038A1 (en) A fermentation process for producing natamycin
ES8105149A1 (en) Process of producing A-42355 antibiotic mixture and A-30912 antibiotics as well as a pure culture of the producing micro organism.
EP0249432A3 (en) Transformation and foreign gene expression with plant species
ES2007950A6 (en) Process for producing novel yeast and mold inhibiting products.
EP0340783A3 (en) Apparatus for culturing animal cells, method of culturing thereof and diagnostics of the culture
CA2092849A1 (en) Callus cell induction and the preparation of taxanes
EP0202667A3 (en) Method of regenerating rice plant from protoplasts
EP0379395A3 (en) Antibiotic l53-18a and process for preparation thereof
NO158814C (en) PROCEDURE FOR THE PREPARATION OF ANTIBIOTIC BY THE FERMENTATION OF KIBDEL PORANGIUM ARIDUM SHEARER GEN. NOV., SP. NOV. ATCC 39322.
DE3666458D1 (en) Method and device for the _i(in vitro) culture of plant cells and organs, as well as for the micropropagation and regeneration of complete plants
NZ522337A (en) Production of TcR gamma delta T cells
AU2742684A (en) Propagation of proliferating cell aggregates
EP0338716A3 (en) Method and system for the production of non-degraded proteins from mammalian cells
GB2162198B (en) Process for producing dense nutrient medium for culturing microorganisms and macroorganism cell cultures
EP0249772A3 (en) Method and composition for plant tissue and cell culture
AU6750787A (en) Process for regenerating corn
UA8653A1 (en) METHOD FOR BACTERIOrhodopsin OBTAINING
DE3169901D1 (en) Antibiotic am-5344-a2 and process for the preparation thereof
ES8304202A1 (en) Process for preparing narasin.

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed