CA2064697A1 - A process for the production, drying and germination of conifer somatic embryos - Google Patents

A process for the production, drying and germination of conifer somatic embryos

Info

Publication number
CA2064697A1
CA2064697A1 CA2064697A CA2064697A CA2064697A1 CA 2064697 A1 CA2064697 A1 CA 2064697A1 CA 2064697 A CA2064697 A CA 2064697A CA 2064697 A CA2064697 A CA 2064697A CA 2064697 A1 CA2064697 A1 CA 2064697A1
Authority
CA
Canada
Prior art keywords
germination
somatic embryos
embryos
drying
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2064697A
Other languages
French (fr)
Other versions
CA2064697C (en
Inventor
Dane Richard Roberts
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BC Research Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2064697A1 publication Critical patent/CA2064697A1/en
Application granted granted Critical
Publication of CA2064697C publication Critical patent/CA2064697C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S47/00—Plant husbandry
    • Y10S47/09—Physical and chemical treatment of seeds for planting

Abstract

A process to assist in germination of spruce somatic embryos. The embryos are partially dried at humidities of less than about 99.9 %, preferably in the range of about 85 to about 99 %. A process that differentiates somatic embryos of conifer com-prises contacting embryogenic calli with a medium containing abscisic acid. A process for determining the quality of a plant to embryo is also described. That process comprises identifying the storage protein content of the embryo and comparing that con-tent with the storage protein content of mature embryos of the same species.
CA002064697A 1989-08-01 1990-07-27 A process for the production, drying and germination of conifer somatic embryos Expired - Lifetime CA2064697C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US388,598 1989-08-01
US07/388,598 US5183757A (en) 1989-08-01 1989-08-01 Process for the production, desiccation and germination of conifer somatic embryos

Publications (2)

Publication Number Publication Date
CA2064697A1 true CA2064697A1 (en) 1991-02-02
CA2064697C CA2064697C (en) 1996-04-23

Family

ID=23534763

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002064697A Expired - Lifetime CA2064697C (en) 1989-08-01 1990-07-27 A process for the production, drying and germination of conifer somatic embryos

Country Status (6)

Country Link
US (1) US5183757A (en)
AU (1) AU6073290A (en)
CA (1) CA2064697C (en)
GB (1) GB2250674B (en)
SE (1) SE500404C2 (en)
WO (1) WO1991001629A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6200809B1 (en) * 1998-03-17 2001-03-13 Cellfor Inc. Maturation of somatic embryos

Families Citing this family (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6340594B1 (en) 1991-12-19 2002-01-22 Cellfor, Inc. Production of desiccation-tolerant gymnosperm embryos
DE69231890T2 (en) 1991-12-19 2002-03-28 Univ Saskatchewan Saskatchewan DESICCATION OF GYMNOSPERM SOMATIC EMBRYONS
US5413930A (en) * 1993-10-21 1995-05-09 Westvaco Corporation Method for regeneration of coniferous plants by somatic embryogenesis
US5506136A (en) * 1993-10-21 1996-04-09 Westvaco Corporation Method for regeneration of coniferous plants by somatic embryogenesis
US5491090A (en) * 1994-02-09 1996-02-13 Westvaco Corporation Embryogenic coniferous liquid suspension cultures
NZ272210A (en) 1995-05-25 1997-10-24 Carter Holt Harvey Ltd Treatment of somatic embryos to provide viable embryos after storage periods
US5677185A (en) * 1996-05-14 1997-10-14 Westvaco Corporation Method for regeneration of coniferous plants by somatic embryogenesis in culture media containing abscisic acid
US5731203A (en) * 1996-06-14 1998-03-24 Westvaco Corporation Method for regeneration of coniferous plants by somatic embryogenesis
US5731191A (en) * 1996-12-20 1998-03-24 Westvaco Corporation Method for regeneration of coniferous plants by somatic embryogenesis employing polyethylene glycol
US5731204A (en) * 1996-12-20 1998-03-24 Westvaco Corporation Method for regeneration of coniferous plants by somatic embryogenesis employing polyethylene glycol
AU739097B2 (en) * 1997-04-21 2001-10-04 Weyerhaeuser Company Method for inducing and determining maturity in conifer somatic embryos
AU778880B2 (en) * 1997-04-21 2004-12-23 Weyerhaeuser Company Method for inducing and determining maturity in conifer somatic embryos
US6143563A (en) * 1997-05-20 2000-11-07 Pioneer Hi-Bred International, Inc. Cryopreservation of embryogenic callus
US6134830A (en) * 1997-11-20 2000-10-24 Weyerhaeuser Company Method for storing and improving the survival rate of conifer somatic embryo germinants
US20040072143A1 (en) * 1998-06-01 2004-04-15 Weyerhaeuser Company Methods for classification of somatic embryos
CA2333184C (en) * 1998-06-01 2013-11-26 Weyerhaeuser Company Methods for classification of somatic embryos
CA2240135A1 (en) 1998-06-05 1999-12-05 University Of Saskatchewan Technologies Inc. Increasing concentration of growth regulator during development of somatic embryos
WO1999065293A1 (en) 1998-06-12 1999-12-23 Silvagen Inc. A process for production and subsequent ex vitro sowing and propagation of pre-germinated plant somatic embryos
US6946295B2 (en) 1998-06-12 2005-09-20 Cellfor, Inc. Process for ex vitro sowing and germination of plant somatic embryos
AU776443B2 (en) 1999-04-15 2004-09-09 Arborgen Inc. Enhancing germination of plant somatic embryos by priming
US6689609B1 (en) 1999-04-15 2004-02-10 Cellfor Inc. Enhancing germination of plant somatic embryos by priming
US6682931B2 (en) 1999-05-25 2004-01-27 Meadwestvaco Corporation Recovering cryopreserved conifer embryogenic cultures
US7598073B2 (en) 2002-04-09 2009-10-06 Weyerhaeuser Nr Company Methods for producing high yields of zygotic-like cotyledonary pine embryos utilizing media that include a disaccharide and glucose
US7381562B2 (en) * 2002-05-30 2008-06-03 Weyerhaeuser Company Methods for producing cotyledonary pine embryos utilizing a gibberellin
CA2435337C (en) * 2002-11-14 2010-03-23 Weyerhaeuser Company Methods for producing conifer somatic embryos
US7521237B2 (en) * 2003-06-23 2009-04-21 Weyerhaeuser Nr Company Methods for promoting maturation of conifer somatic embryos
US20040266002A1 (en) * 2003-06-30 2004-12-30 Weyerhaeuser Company Use of abscisic acid in somatic embryogenesis of pine trees
US7881502B2 (en) 2003-06-30 2011-02-01 Weyerhaeuser Nr Company Method and system for three-dimensionally imaging an apical dome of a plant embryo
US7530197B2 (en) 2003-06-30 2009-05-12 Weyerhaeuser Co. Automated system and method for harvesting and multi-stage screening of plant embryos
US7732205B2 (en) * 2003-07-30 2010-06-08 Weyerhaeuser Nr Company Development and stratification of pine somatic embryos using a liquid system
US7228658B2 (en) * 2003-08-27 2007-06-12 Weyerhaeuser Company Method of attaching an end seal to manufactured seeds
US8691575B2 (en) * 2003-09-30 2014-04-08 Weyerhaeuser Nr Company General method of classifying plant embryos using a generalized Lorenz-Bayes classifier
US7555865B2 (en) * 2003-11-25 2009-07-07 Weyerhaeuser Nr Company Method and system of manufacturing artificial seed coats
US20050108935A1 (en) * 2003-11-25 2005-05-26 Edwin Hirahara Method and system of manufacturing artificial seed coats
CA2484533C (en) * 2003-11-25 2008-12-02 Weyerhaeuser Company Systems and method of embryo delivery for manufactured seeds
CA2486289C (en) * 2003-11-25 2008-01-08 Weyerhaeuser Company Combination end seal and restraint
US20050108929A1 (en) * 2003-11-25 2005-05-26 Edwin Hirahara Method and system for creating manufactured seeds
US7520089B2 (en) * 2003-11-25 2009-04-21 Weyerhaeuser Nr Company Method to improve manufactured seed germination
CA2486311C (en) 2003-11-26 2008-08-12 Weyerhaeuser Company Vacuum pick-up device with mechanically assisted release
US7795029B2 (en) * 2003-12-04 2010-09-14 Cellfor Inc. Method of ex vitro sowing, germination, growth and conversion of plant somatic embryos or germinants
US7356965B2 (en) * 2003-12-11 2008-04-15 Weyerhaeuser Co. Multi-embryo manufactured seed
US7591287B2 (en) * 2003-12-18 2009-09-22 Weyerhaeuser Nr Company System and method for filling a seedcoat with a liquid to a selected level
CA2495650C (en) * 2004-02-25 2011-05-24 Weyerhaeuser Company Continuous culture of conifer embryogenic tissue
CA2496880C (en) * 2004-03-02 2011-01-18 Weyerhaeuser Company Methods for developing conifer somatic embryos
US7568309B2 (en) * 2004-06-30 2009-08-04 Weyerhaeuser Nr Company Method and system for producing manufactured seeds
US20060064930A1 (en) * 2004-09-27 2006-03-30 Carlson William C Manufactured seed having a live end seal coating
US7547488B2 (en) * 2004-12-15 2009-06-16 Weyerhaeuser Nr Company Oriented strand board panel having improved strand alignment and a method for making the same
WO2006094400A1 (en) * 2005-03-10 2006-09-14 Cellfor Inc. Aerated liquid priming of conifer somatic germinants
WO2006118962A2 (en) * 2005-04-29 2006-11-09 Arborgen, Llc Improved somatic embryogenesis and embryo harvesting and method and apparatus for preparing plant embryos for plant production
US7654037B2 (en) * 2005-06-30 2010-02-02 Weyerhaeuser Nr Company Method to improve plant somatic embryo germination from manufactured seed
US20070087438A1 (en) * 2005-10-17 2007-04-19 Grob James A Methods for conditioning plant somatic embryos
US7719099B2 (en) * 2005-10-21 2010-05-18 Advanced Optoelectronic Technology Inc. Package structure for solid-state lighting devices and method of fabricating the same
CA2568476C (en) * 2005-12-22 2015-03-17 Weyerhaeuser Company Methods for storing conifer somatic embryo germinants
US8744775B2 (en) * 2007-12-28 2014-06-03 Weyerhaeuser Nr Company Methods for classification of somatic embryos comprising hyperspectral line imaging
US9055721B2 (en) 2010-08-19 2015-06-16 The Institute For Advanced Learning And Research Methods and media formulations for large-scale and efficient micropropagation of bio-energy grasses
CN102771393B (en) * 2012-08-02 2013-10-16 中国林业科学研究院林业研究所 Method for picea balfouriana somatic embryo generation and plant regeneration
CN113575418A (en) * 2021-08-17 2021-11-02 青岛农业大学 Device and culture method for drying treatment of Pinus sylvestris in vitro embryos

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4777762A (en) * 1986-01-07 1988-10-18 Plant Genetics, Inc. Desiccated analogs of botanic seed
GB8717099D0 (en) * 1987-07-20 1987-08-26 Univ Guelph Induce desiccation tolerance in somatic embryos
EP0397665A1 (en) * 1987-12-18 1990-11-22 University Of Florida Quiescent plant somatic embryos and method for their production
JPH02504589A (en) * 1988-06-14 1990-12-27 プラント・ジェネティックス・インコーポレーテッド How to mature somatic embryos for planting in a natural environment
US4957866A (en) * 1989-03-09 1990-09-18 Weyerhaeuser Company Method for reproducing coniferous plants by somatic embryogenesis

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6200809B1 (en) * 1998-03-17 2001-03-13 Cellfor Inc. Maturation of somatic embryos

Also Published As

Publication number Publication date
GB2250674A (en) 1992-06-17
SE9200269L (en) 1992-03-27
GB2250674B (en) 1994-03-02
AU6073290A (en) 1991-03-11
US5183757A (en) 1993-02-02
SE9200269D0 (en) 1992-01-31
SE500404C2 (en) 1994-06-20
CA2064697C (en) 1996-04-23
WO1991001629A1 (en) 1991-02-21
GB9201986D0 (en) 1992-03-18

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