CA2107463A1 - Totipotent hematopoietic stem cell receptors and their ligands - Google Patents

Totipotent hematopoietic stem cell receptors and their ligands

Info

Publication number
CA2107463A1
CA2107463A1 CA002107463A CA2107463A CA2107463A1 CA 2107463 A1 CA2107463 A1 CA 2107463A1 CA 002107463 A CA002107463 A CA 002107463A CA 2107463 A CA2107463 A CA 2107463A CA 2107463 A1 CA2107463 A1 CA 2107463A1
Authority
CA
Canada
Prior art keywords
ligands
flk
nucleic acid
expressed
receptor protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002107463A
Other languages
French (fr)
Other versions
CA2107463C (en
Inventor
Ihor R. Lemischka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Princeton University
Original Assignee
Ihor R. Lemischka
The Trustees Of Princeton University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ihor R. Lemischka, The Trustees Of Princeton University filed Critical Ihor R. Lemischka
Publication of CA2107463A1 publication Critical patent/CA2107463A1/en
Application granted granted Critical
Publication of CA2107463C publication Critical patent/CA2107463C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/02—Acyclic radicals, not substituted by cyclic structures
    • C07H15/12—Acyclic radicals, not substituted by cyclic structures attached to a nitrogen atom of the saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70596—Molecules with a "CD"-designation not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Abstract

Isolated mammalian nucleic acid molecules encoding receptor protein tyrosine kinases expressed in primitive hematopoie-tic cells and not expressed in mature hematopoietic cells are provided. Also included are the receptors encoded by such nucleic acid molecules; the nucleic acid molecules encoding receptor protein tyrosine kinases having the sequences shown in Figure 1 (flk-2) and Figure 2(flk-1); the receptor protein tyrosine kinases having the amino acid sequences shown in Figure 1 (flk-2) and Figure 2 (flk-1); ligands for the receptors; nucleic acid sequences that encode the ligands; and methods of stimulating the prolif-eration and/or differentiation of primitive mammalian hematopoietic stem cells comprising contacting the stem cells with a li-gand that binds to a receptor protein tyrosine kinase expressed in primitive mammalian hematopoietic cells and not expressed in mature hematopoietic cells.
CA002107463A 1991-04-02 1992-04-02 Totipotent hematopoietic stem cell receptors and their ligands Expired - Lifetime CA2107463C (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US67966691A 1991-04-02 1991-04-02
US07/679,666 1991-04-02
US72891391A 1991-06-28 1991-06-28
US07/728,913 1991-06-28
US79306591A 1991-11-15 1991-11-15
US07/793,065 1991-11-15
US07/813,593 US5185438A (en) 1991-04-02 1991-12-24 Nucleic acids encoding hencatoporetic stem cell receptor flk-2
US07/813,593 1991-12-24
PCT/US1992/002750 WO1992017486A1 (en) 1991-04-02 1992-04-02 Totipotent hematopoietic stem cell receptors and their ligands

Publications (2)

Publication Number Publication Date
CA2107463A1 true CA2107463A1 (en) 1992-10-03
CA2107463C CA2107463C (en) 2008-06-17

Family

ID=27505386

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002107463A Expired - Lifetime CA2107463C (en) 1991-04-02 1992-04-02 Totipotent hematopoietic stem cell receptors and their ligands

Country Status (7)

Country Link
US (3) US5185438A (en)
EP (6) EP1231261B1 (en)
KR (3) KR100261465B1 (en)
AU (1) AU1924892A (en)
CA (1) CA2107463C (en)
DE (5) DE69232952T2 (en)
WO (2) WO1992017486A1 (en)

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Also Published As

Publication number Publication date
DE69233788D1 (en) 2010-08-05
EP0580760A4 (en) 1997-04-09
KR100406832B1 (en) 2003-11-21
WO1992017486A1 (en) 1992-10-15
EP1231265A2 (en) 2002-08-14
DE69232952D1 (en) 2003-04-17
DE69232687D1 (en) 2002-08-22
US5283354A (en) 1994-02-01
DE69233676D1 (en) 2007-03-22
EP1231261A3 (en) 2003-12-03
KR100261465B1 (en) 2000-07-01
EP1231261B1 (en) 2010-06-23
DE69233746D1 (en) 2008-10-30
CA2107463C (en) 2008-06-17
DE69232952T2 (en) 2003-10-02
US5621090A (en) 1997-04-15
EP1231265B1 (en) 2007-01-31
EP0909813B1 (en) 2002-07-17
DE69232687T2 (en) 2002-11-21
EP1808445A2 (en) 2007-07-18
EP0580760A1 (en) 1994-02-02
EP0580760B1 (en) 2003-03-12
WO1993000349A1 (en) 1993-01-07
EP1231260B1 (en) 2008-09-17
EP1231261A2 (en) 2002-08-14
DE69233676T2 (en) 2007-11-15
EP1231265A3 (en) 2003-12-03
EP0909813A1 (en) 1999-04-21
US5185438A (en) 1993-02-09
EP1808445A3 (en) 2007-09-26
KR100407010B1 (en) 2003-11-28
AU1924892A (en) 1992-11-02
EP1231260A2 (en) 2002-08-14
EP1231260A3 (en) 2003-12-03

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