CA2113907A1 - Apparatus and methods for electroporation amd electrofusion - Google Patents

Apparatus and methods for electroporation amd electrofusion

Info

Publication number
CA2113907A1
CA2113907A1 CA002113907A CA2113907A CA2113907A1 CA 2113907 A1 CA2113907 A1 CA 2113907A1 CA 002113907 A CA002113907 A CA 002113907A CA 2113907 A CA2113907 A CA 2113907A CA 2113907 A1 CA2113907 A1 CA 2113907A1
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Canada
Prior art keywords
cells
electrofusion
pores
amd
electroporation
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CA002113907A
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French (fr)
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CA2113907C (en
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Robert E. Schmukler
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Publication of CA2113907A1 publication Critical patent/CA2113907A1/en
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Publication of CA2113907C publication Critical patent/CA2113907C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Sustainable Development (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Electromagnetism (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Branch Pipes, Bends, And The Like (AREA)
  • Lining Or Joining Of Plastics Or The Like (AREA)
  • Vaporization, Distillation, Condensation, Sublimation, And Cold Traps (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

An insulating film (20) in a container has pores (28) whose diameter is smaller than the diameter of a first type of cells (60) in the container. The first type of cells are trapped in the pores, so that when an electric field is applied to the container, electro-poration of the first cells occurs. If a second type of cells (70), smaller than the first type of cells, are also trapped in the pores, electrofusion will occur between the first and second types of cells trapped in the same pores.
CA002113907A 1991-07-22 1992-07-21 Apparatus and methods for electroporation amd electrofusion Expired - Fee Related CA2113907C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US733,854 1991-07-22
US07/733,854 US5173158A (en) 1991-07-22 1991-07-22 Apparatus and methods for electroporation and electrofusion
PCT/US1992/005855 WO1993002178A1 (en) 1991-07-22 1992-07-21 Apparatus and methods for electroporation and electrofusion

Publications (2)

Publication Number Publication Date
CA2113907A1 true CA2113907A1 (en) 1993-02-04
CA2113907C CA2113907C (en) 2002-12-24

Family

ID=24949376

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002113907A Expired - Fee Related CA2113907C (en) 1991-07-22 1992-07-21 Apparatus and methods for electroporation amd electrofusion

Country Status (7)

Country Link
US (2) US5173158A (en)
EP (1) EP0596003B1 (en)
AT (1) ATE176278T1 (en)
AU (1) AU2378092A (en)
CA (1) CA2113907C (en)
DE (1) DE69228309T2 (en)
WO (1) WO1993002178A1 (en)

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US5283194A (en) 1994-02-01
EP0596003A1 (en) 1994-05-11
DE69228309T2 (en) 1999-09-16
CA2113907C (en) 2002-12-24
US5173158A (en) 1992-12-22
DE69228309D1 (en) 1999-03-11
EP0596003A4 (en) 1995-12-06
EP0596003B1 (en) 1999-01-27
WO1993002178A1 (en) 1993-02-04
ATE176278T1 (en) 1999-02-15
AU2378092A (en) 1993-02-23

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