CA2118248A1 - Apparatus for separating a mixture - Google Patents

Apparatus for separating a mixture

Info

Publication number
CA2118248A1
CA2118248A1 CA002118248A CA2118248A CA2118248A1 CA 2118248 A1 CA2118248 A1 CA 2118248A1 CA 002118248 A CA002118248 A CA 002118248A CA 2118248 A CA2118248 A CA 2118248A CA 2118248 A1 CA2118248 A1 CA 2118248A1
Authority
CA
Canada
Prior art keywords
electrode structure
flow
electrodes
chamber
eluant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002118248A
Other languages
French (fr)
Other versions
CA2118248C (en
Inventor
Walter B. Betts
Jeremy J. Hawkes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BTG International Ltd
Original Assignee
Walter B. Betts
Jeremy J. Hawkes
British Technology Group Limited
Btg International Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Walter B. Betts, Jeremy J. Hawkes, British Technology Group Limited, Btg International Limited filed Critical Walter B. Betts
Publication of CA2118248A1 publication Critical patent/CA2118248A1/en
Application granted granted Critical
Publication of CA2118248C publication Critical patent/CA2118248C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
    • B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Abstract

Apparatus for separating a mixture of particles comprising a chamber with an inlet (3) and an outlet (4), means for providing an eluant flow between said inlet (3) and said outlet (4), within the chamber an electrode structure (4) of at least two electrodes, means (10) for applying an alternating voltage between the electrodes, said electrode structure being such that when energized in operation of the apparatus there is established in the eluant a spatially non-uniform alternating field, whereby particles caused to flow through the chamber in said eluant flow are carried past the electrode structure and are separated into fractions.
There may be an ancillary electrode structure (15) of at least two electrodes placed upstream of the main electrode structure (2) with regard to the direction of the flow of fluid. At least one of the electrodes of the electrode structure (2) may be comprised of one or more grid like structures (53, 54). The electrode structure (2) may be comprised of interleaved grid like structures (75, 76) aligned to obstruct straight through flow.
CA002118248A 1992-04-16 1993-04-15 Apparatus for separating a mixture Expired - Lifetime CA2118248C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB9208357.5 1992-04-16
GB929208357A GB9208357D0 (en) 1992-04-16 1992-04-16 Apparatus for separating a mixture
PCT/GB1993/000793 WO1993020927A1 (en) 1992-04-16 1993-04-15 Apparatus for separating a mixture

Publications (2)

Publication Number Publication Date
CA2118248A1 true CA2118248A1 (en) 1993-10-28
CA2118248C CA2118248C (en) 2005-09-20

Family

ID=10714119

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002118248A Expired - Lifetime CA2118248C (en) 1992-04-16 1993-04-15 Apparatus for separating a mixture

Country Status (11)

Country Link
US (1) US5569367A (en)
EP (1) EP0646040B1 (en)
JP (1) JP3182151B2 (en)
KR (1) KR950700783A (en)
AT (1) ATE176601T1 (en)
AU (1) AU4076893A (en)
CA (1) CA2118248C (en)
DE (1) DE69323488T2 (en)
ES (1) ES2127273T3 (en)
GB (2) GB9208357D0 (en)
WO (1) WO1993020927A1 (en)

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US5888370A (en) 1996-02-23 1999-03-30 Board Of Regents, The University Of Texas System Method and apparatus for fractionation using generalized dielectrophoresis and field flow fractionation
US5993630A (en) * 1996-01-31 1999-11-30 Board Of Regents The University Of Texas System Method and apparatus for fractionation using conventional dielectrophoresis and field flow fractionation
CA2245514C (en) * 1996-01-31 2006-05-23 Board Of Regents, The University Of Texas System Fractionation using dielectrophoresis and field flow fractionation
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US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
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US6352838B1 (en) * 1999-04-07 2002-03-05 The Regents Of The Universtiy Of California Microfluidic DNA sample preparation method and device
AU4773400A (en) * 1999-05-28 2000-12-18 Leisamon (Israel), Ltd. Methods and apparatus for nonlinear mobility electrophoresis separation
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US6537433B1 (en) 2000-03-10 2003-03-25 Applera Corporation Methods and apparatus for the location and concentration of polar analytes using an alternating electric field
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US6787018B1 (en) 2000-12-08 2004-09-07 The Regents Of The University Of California Dielectrophoretic concentration of particles under electrokinetic flow
US6764583B2 (en) * 2000-12-13 2004-07-20 The Regents Of The University Of California Using impedance measurements for detecting pathogens trapped in an electric field
US7014744B2 (en) * 2001-08-24 2006-03-21 Applera Corporation Method of purification and concentration using AC fields with a transfer tip
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US6703819B2 (en) 2001-12-03 2004-03-09 Board Of Regents, The University Of Texas System Particle impedance sensor
US6866762B2 (en) * 2001-12-20 2005-03-15 Board Of Regents, University Of Texas System Dielectric gate and methods for fluid injection and control
US20030119057A1 (en) * 2001-12-20 2003-06-26 Board Of Regents Forming and modifying dielectrically-engineered microparticles
US6887362B2 (en) * 2002-02-06 2005-05-03 Nanogen, Inc. Dielectrophoretic separation and immunoassay methods on active electronic matrix devices
GB2392977A (en) * 2002-09-13 2004-03-17 Suisse Electronique Microtech A fluidic dielectrophoretic system and method for analysing biomolecules
US7184791B2 (en) * 2002-09-23 2007-02-27 Telefonaktiebolaget Lm Ericsson (Publ) Methods, receivers, and computer program products for determining transmission power control commands using biased interpretation
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US7147764B2 (en) 2003-03-28 2006-12-12 Applera Corporation Dual electrode injection of analyte into a capillary electrophoretic device
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US7309410B2 (en) * 2003-12-03 2007-12-18 Palo Alto Research Center Incorporated Traveling wave grids and algorithms for biomolecule separation, transport and focusing
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US8702945B2 (en) * 2007-05-18 2014-04-22 University Of Washington Time-varying flows for microfluidic particle separation
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GB0804491D0 (en) * 2008-03-11 2008-04-16 Iti Scotland Ltd Detecting analytes
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GB0809486D0 (en) * 2008-05-23 2008-07-02 Iti Scotland Ltd Triple function elctrodes
TWI372137B (en) * 2009-09-04 2012-09-11 Univ Nat Chiao Tung Dielectrophoresis-based microfluidic system
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KR101359708B1 (en) * 2012-04-04 2014-02-10 글로벌광통신 (주) microorganism measurement apparatus
GB2561194A (en) * 2017-04-04 2018-10-10 Galloway Roma Quick count device
US10888875B2 (en) * 2017-06-16 2021-01-12 Regents Of The University Of Minnesota Electrodes formed from 2D materials for dielectrophoresis and systems and methods for utilizing the same
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Also Published As

Publication number Publication date
AU4076893A (en) 1993-11-18
WO1993020927A1 (en) 1993-10-28
DE69323488D1 (en) 1999-03-25
CA2118248C (en) 2005-09-20
GB9208357D0 (en) 1992-06-03
GB2266153A (en) 1993-10-20
JP3182151B2 (en) 2001-07-03
GB9307770D0 (en) 1993-06-02
US5569367A (en) 1996-10-29
DE69323488T2 (en) 1999-06-24
EP0646040A1 (en) 1995-04-05
KR950700783A (en) 1995-02-20
EP0646040B1 (en) 1999-02-10
ES2127273T3 (en) 1999-04-16
JPH07505717A (en) 1995-06-22
ATE176601T1 (en) 1999-02-15
GB2266153B (en) 1996-11-06

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Legal Events

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EEER Examination request