CA2135073A1 - Nucleic acid sequence amplification method, composition and kit - Google Patents

Nucleic acid sequence amplification method, composition and kit

Info

Publication number
CA2135073A1
CA2135073A1 CA002135073A CA2135073A CA2135073A1 CA 2135073 A1 CA2135073 A1 CA 2135073A1 CA 002135073 A CA002135073 A CA 002135073A CA 2135073 A CA2135073 A CA 2135073A CA 2135073 A1 CA2135073 A1 CA 2135073A1
Authority
CA
Canada
Prior art keywords
nucleic acid
kit
composition
primer
target sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002135073A
Other languages
French (fr)
Other versions
CA2135073C (en
Inventor
Daniel L. Kacian
Diane L. Mcallister
Sherrol H. Mcdonough
Nanibhushan Dattagupta
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gen Probe Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2135073A1 publication Critical patent/CA2135073A1/en
Application granted granted Critical
Publication of CA2135073C publication Critical patent/CA2135073C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Abstract

A method, composition and kit for amplifying a target nucleic acid sequence under conditions of substantially constant temperature, ionic strength, and pH and using only a single promoter-primer.
To effect the amplification, a supply of a single promoter-primer having a promoter and a primer complementary to the 3'-end of the target sequence, and a reserve transcriptase and an RNA polymerase are provided to a mixture including the target sequence;
the amplification proceeds accordingly. The in-vention is useful for generating copies of a nucleic acid target sequence for purposes that include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.
CA002135073A 1992-05-06 1993-04-29 Nucleic acid sequence amplification method, composition and kit Expired - Lifetime CA2135073C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US87968692A 1992-05-06 1992-05-06
US07/879,686 1992-05-06
PCT/US1993/004015 WO1993022461A1 (en) 1992-05-06 1993-04-29 Nucleic acid sequence amplification method, composition and kit

Publications (2)

Publication Number Publication Date
CA2135073A1 true CA2135073A1 (en) 1993-11-11
CA2135073C CA2135073C (en) 2002-11-19

Family

ID=25374679

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002135073A Expired - Lifetime CA2135073C (en) 1992-05-06 1993-04-29 Nucleic acid sequence amplification method, composition and kit

Country Status (12)

Country Link
US (2) US5554516A (en)
EP (1) EP0587266B1 (en)
JP (1) JP4137996B2 (en)
KR (1) KR100249110B1 (en)
AT (1) ATE307898T1 (en)
AU (1) AU681082B2 (en)
CA (1) CA2135073C (en)
DE (1) DE69333891T2 (en)
DK (1) DK0587266T3 (en)
ES (1) ES2250960T3 (en)
TW (1) TW360712B (en)
WO (1) WO1993022461A1 (en)

Families Citing this family (295)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7009041B1 (en) 1989-07-11 2006-03-07 Gen-Probe Incorporated Oligonucleotides for nucleic acid amplification and for the detection of Mycobacterium tuberculosis
US5766849A (en) * 1989-07-11 1998-06-16 Gen-Probe Incorporated Methods of amplifying nucleic acids using promoter-containing primer sequence
US6093538A (en) * 1992-05-06 2000-07-25 Gen-Probe Incorporated Nucleic acid probes to ureaplasma
AU670116B2 (en) * 1992-08-04 1996-07-04 Gen-Probe Incorporated Nucleic acid sequence amplification
US20050123926A1 (en) * 1994-01-13 2005-06-09 Enzo Diagnostics, Inc., In vitro process for producing multiple nucleic acid copies
US20110097791A1 (en) * 1999-04-16 2011-04-28 Engelhardt Dean L Novel process, construct and conjugate for producing multiple nucleic acid copies
US5656427A (en) * 1994-08-29 1997-08-12 Gen-Probe Incorporated Nucleic acid hybridization assay probes, helper probes and amplification oligonucleotides targeted to Mycoplasma pneumoniae nucleic acid
US5514551A (en) * 1994-10-14 1996-05-07 Gen-Probe Incorporated Compositions for the detection of Chlamydia trachomatis
US5622827A (en) * 1995-02-07 1997-04-22 Gen-Probe Incorporated Amplification primers and nucleic acid probes targeted to coccidioides immitis nucleic acid
US5932450A (en) * 1995-06-07 1999-08-03 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using digestible templates
US5916777A (en) * 1995-06-07 1999-06-29 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide primers
WO1996040990A1 (en) * 1995-06-07 1996-12-19 Gen-Probe Incorporated Methods and kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5739311A (en) * 1995-06-07 1998-04-14 Gen-Probe Incorporated Enzymatic synthesis of phosphorothioate oligonucleotides using restriction endonucleases
US5705365A (en) * 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5882867A (en) * 1995-06-07 1999-03-16 Dade Behring Marburg Gmbh Detection of nucleic acids by formation of template-dependent product
US5652126A (en) * 1995-06-07 1997-07-29 Gen-Probe Incorporated Use of restriction endonuclease sequences for cleaving phosphorothioate oligonucleotides
US5710029A (en) * 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US6218107B1 (en) 1996-05-22 2001-04-17 Gen-Probe Incorporated Compositions and methods for detecting the presence of Mycobacterium kansassii
US5919638A (en) * 1996-10-08 1999-07-06 Abbott Laboratories Reagents and methods useful for detecting prostate tumors
US20050202499A1 (en) 1996-10-31 2005-09-15 Billing-Medel Patricia A. Reagents and methods useful for detecting diseases of the breast
US5837466A (en) * 1996-12-16 1998-11-17 Vysis, Inc. Devices and methods for detecting nucleic acid analytes in samples
US6025133A (en) * 1996-12-30 2000-02-15 Gen-Probe Incorporated Promoter-sequestered oligonucleoside and method of use
JP3968810B2 (en) * 1997-01-24 2007-08-29 東ソー株式会社 Nucleic acid sequence analysis method
WO1998045420A1 (en) 1997-04-10 1998-10-15 Diagnocure Inc. Pca3, pca3 genes, and methods of use
US6558901B1 (en) * 1997-05-02 2003-05-06 Biomerieux Vitek Nucleic acid assays
ES2271994T3 (en) * 1997-05-02 2007-04-16 Gen-Probe Incorporated TWO-STEP HYBRIDIZATION AND CAPTURE OF A POLINUCLEOTIDE.
US6534273B2 (en) 1997-05-02 2003-03-18 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
US6429007B1 (en) * 1997-05-02 2002-08-06 BIOMéRIEUX, INC. Nucleic acid amplification reaction station for disposable test devices
IL124275A (en) * 1997-05-02 2002-03-10 Bio Merieux Vitek Inc Method for generating nucleic acid sequences
US5989499A (en) * 1997-05-02 1999-11-23 Biomerieux, Inc. Dual chamber disposable reaction vessel for amplification reactions
US5786182A (en) * 1997-05-02 1998-07-28 Biomerieux Vitek, Inc. Dual chamber disposable reaction vessel for amplification reactions, reaction processing station therefor, and methods of use
US6410275B1 (en) 1997-05-02 2002-06-25 Biomerieux, Inc. Disposable test devices for performing nucleic acid amplification reactions
ES2153807T3 (en) 1997-08-08 2005-06-16 Biomerieux B.V. SEQUENCES OF NUCLEIC ACID THAT CAN BE USED AS PRIMERS AND PROBES IN THE AMPLIFICATION AND DETECTION OF ALL HIV-1 SUBTIPOS.
JP2002510465A (en) 1998-01-27 2002-04-09 サイトセル・リミテッド Modified nucleic acid probes and uses thereof
US20040002079A1 (en) * 1998-02-11 2004-01-01 Gunn Robert B. Sodium-phosphate cotransporter in lithium therapy for the treatment of mental illness
US6365346B1 (en) * 1998-02-18 2002-04-02 Dade Behring Inc. Quantitative determination of nucleic acid amplification products
EP1170381A3 (en) * 1998-02-27 2004-01-28 PamGene B.V. Method for the non-specific amplification of nucleic acid
DE69936897T2 (en) 1998-05-01 2008-05-15 Gen-Probe Inc., San Diego Incubator for automatic analyzer
US8337753B2 (en) 1998-05-01 2012-12-25 Gen-Probe Incorporated Temperature-controlled incubator having a receptacle mixing mechanism
WO1999067641A2 (en) 1998-06-24 1999-12-29 Illumina, Inc. Decoding of array sensors with microspheres
JP4438110B2 (en) 1998-07-01 2010-03-24 東ソー株式会社 Quantification method of target nucleic acid
EP1100972B1 (en) * 1998-07-31 2006-06-14 Gen-Probe Incorporated Reversible inhibiting probes
JP4495349B2 (en) 1999-01-28 2010-07-07 ジェン−プローブ・インコーポレーテッド Nucleic acid sequences for detecting cancer genetic markers in biological samples
US6846460B1 (en) 1999-01-29 2005-01-25 Illumina, Inc. Apparatus and method for separation of liquid phases of different density and for fluorous phase organic syntheses
EP1218536A2 (en) 1999-02-12 2002-07-03 Genset Biallelic markers derived from genomic regions carrying genes involved in arachidonic acid metabolism
US6582906B1 (en) 1999-04-05 2003-06-24 Affymetrix, Inc. Proportional amplification of nucleic acids
US20030207295A1 (en) * 1999-04-20 2003-11-06 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US6355431B1 (en) * 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US6132997A (en) 1999-05-28 2000-10-17 Agilent Technologies Method for linear mRNA amplification
ATE456677T1 (en) * 1999-07-09 2010-02-15 Gen Probe Inc HIV-1 DETECTION USING NUCLEIC ACID AMPLIFICATION
DE60022541T2 (en) 1999-07-23 2006-06-14 Gen Probe Inc POLYNUCLEOTIDE AMPLIFICATION PROCESS
GB9917816D0 (en) * 1999-07-29 1999-09-29 Cytocell Ltd Chemically modified probes used to regulate nucleic acid amplification assays
US6864050B2 (en) 1999-07-30 2005-03-08 Affymetrix, Inc. Single-phase amplification of nucleic acids
US6232104B1 (en) 1999-08-17 2001-05-15 Dade Behring Inc. Detection of differences in nucleic acids by inhibition of spontaneous DNA branch migration
US6692918B2 (en) 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
ES2447419T3 (en) 1999-09-13 2014-03-12 Nugen Technologies, Inc. Compositions for linear isothermal amplification of polynucleotide sequences
CA2385477C (en) 1999-09-29 2009-11-03 Diagnocure Inc. Pca3 messenger rna species in benign and malignant prostate tissues
ATE312204T1 (en) * 1999-12-15 2005-12-15 Gen Probe Inc METHODS AND COMPOSITIONS FOR DETECTING SPECIES OF THE MYCOBACTERIUM AVIUM COMPLEX
US6489114B2 (en) 1999-12-17 2002-12-03 Bio Merieux Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby
ATE495268T1 (en) 1999-12-17 2011-01-15 Gen Probe Inc NUCLEIC ACID AMPLIFICATION AND DETECTION OF MYCOBACTERIUM SPECIES
US6664081B2 (en) 1999-12-17 2003-12-16 Gen-Probe Incorporated Nucleic acid amplification and detection of mycobacterium species
US6902891B2 (en) 1999-12-17 2005-06-07 Bio Merieux Process for labeling a nucleic acid
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
DE60136166D1 (en) 2000-02-07 2008-11-27 Illumina Inc NUCLEIC ACID PROOF METHOD WITH UNIVERSAL PRIMING
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
AU2001238067B2 (en) 2000-02-07 2007-01-25 Illumina, Inc. Nucleic acid detection methods using universal priming
US7361488B2 (en) * 2000-02-07 2008-04-22 Illumina, Inc. Nucleic acid detection methods using universal priming
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
AU2001271595A1 (en) * 2000-06-26 2002-01-08 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US6743592B1 (en) * 2000-07-25 2004-06-01 The Trustees Of The University Of Pennsylvania Methods, systems and kits for immuno-detection of epitopes expressed on molecules
US7524628B2 (en) * 2000-07-25 2009-04-28 The Trustees Of The University Of Pennsylvania Method for detecting molecules expressing a selected epitope via fluorescent dyes
EP1315839B9 (en) 2000-09-01 2009-08-12 Gen-Probe Incorporated Amplification of hiv-1 sequences for detection of sequences associated with drug-resistance mutations
US6582920B2 (en) 2000-09-01 2003-06-24 Gen-Probe Incorporated Amplification of HIV-1 RT sequences for detection of sequences associated with drug-resistance mutations
US6815164B2 (en) 2000-10-06 2004-11-09 Nugen Technologies, Inc. Methods and probes for detection and/or quantification of nucleic acid sequences
WO2002034951A2 (en) 2000-10-23 2002-05-02 Gen-Probe Incorporated Compositions and methods for detecting human immunodeficiency virus 2 (hiv-2)
US20040018491A1 (en) * 2000-10-26 2004-01-29 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
US7229765B2 (en) * 2000-11-28 2007-06-12 Rosetta Inpharmatics Llc Random-primed reverse transcriptase-in vitro transcription method for RNA amplification
DE60142709D1 (en) 2000-12-13 2010-09-09 Nugen Technologies Inc METHODS AND COMPOSITIONS FOR GENERATING A VARIETY OF COPIES OF NUCLEIC ACID SEQUENCES AND METHODS OF DETECTING THE SAME
US6794141B2 (en) 2000-12-22 2004-09-21 Arcturus Bioscience, Inc. Nucleic acid amplification
ZA200210369B (en) 2001-03-09 2004-07-08 Nugen Technologies Inc Methods and compositions for amplification or RNA sequences.
JP2005508135A (en) 2001-03-09 2005-03-31 ニューゲン テクノロジーズ， インコーポレイテッド Methods and compositions for amplification of RNA sequences
US9261460B2 (en) * 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
US7341831B2 (en) * 2001-07-18 2008-03-11 The Trustees Of The University Of Pennsylvania Method for immuno-detection of epitopes
WO2003014151A2 (en) 2001-08-10 2003-02-20 Genset S.A. Human secreted proteins, their encoding polynucleotides, and uses thereof
US7045319B2 (en) 2001-10-30 2006-05-16 Ribomed Biotechnologies, Inc. Molecular detection systems utilizing reiterative oligonucleotide synthesis
US20040054162A1 (en) * 2001-10-30 2004-03-18 Hanna Michelle M. Molecular detection systems utilizing reiterative oligonucleotide synthesis
EP1476573B1 (en) 2002-01-28 2010-01-06 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
ATE405676T1 (en) * 2002-03-11 2008-09-15 Nugen Technologies Inc METHOD FOR GENERATING DOUBLE STRANDED DNA WITH A 3' SINGLE STRAND PORTION AND USES OF THESE COMPLEXES FOR RECOMBINATION
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
US7166478B2 (en) * 2002-03-12 2007-01-23 Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
AU2003220249A1 (en) * 2002-03-15 2003-09-29 Arcturus Bioscience, Inc. Improved nucleic acid amplification
DE10215238C1 (en) * 2002-04-06 2003-08-14 Cytonet Gmbh & Co Kg Detecting mycobacteria and differentiating between Mycobacterium tuberculosis and Mycobacterium avium, comprises amplifying the 16S rRNA gene
US7405062B2 (en) * 2002-05-14 2008-07-29 San Diego Antibody Method for cloning variable domain sequences of immunological gene repertoire
CN103399162B (en) 2002-05-17 2016-04-27 贝克顿·迪金森公司 For separating of, amplify and detect the automated system of target nucleic acid sequence
WO2004011665A2 (en) * 2002-05-17 2004-02-05 Nugen Technologies, Inc. Methods for fragmentation, labeling and immobilization of nucleic acids
EP2292801B1 (en) 2002-06-14 2018-08-08 Gen-Probe Incorporated Compositions and methods for detecting hepatitis b virus
EP2977470B1 (en) 2002-10-16 2018-01-03 Gen-Probe Incorporated Compositions and methods for detecting west nile virus
US7074561B2 (en) * 2002-10-22 2006-07-11 Biomerieux, Inc. Isothermal amplification based assay for the detection and quantitation of alpha-fetoprotein mRNA
US20040101844A1 (en) * 2002-11-21 2004-05-27 Amorese Douglas A. Methods and compositions for producing linearly amplified amounts of (+) strand RNA
WO2004065000A1 (en) 2003-01-21 2004-08-05 Illumina Inc. Chemical reaction monitor
WO2004083806A2 (en) 2003-01-22 2004-09-30 University Of South Florida Autonomous genosensor apparatus and methods for use
ES2427853T3 (en) 2003-02-07 2013-11-04 Diagnocure Inc. Procedure to detect prostate cancer in a sample
US6943768B2 (en) 2003-02-21 2005-09-13 Xtellus Inc. Thermal control system for liquid crystal cell
US8206913B1 (en) 2003-03-07 2012-06-26 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
EP1604040B1 (en) * 2003-03-07 2010-10-13 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process
WO2004092418A2 (en) 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
WO2005031005A2 (en) 2003-05-19 2005-04-07 Gen-Probe Incorporated Compositions, methods and kits for determining the presence of trichomonas vaginalis in a test sample
US20040248102A1 (en) * 2003-06-03 2004-12-09 Diane Ilsley-Tyree Methods and compositions for performing template dependent nucleic acid primer extension reactions that produce a reduced complexity product
JP2007521018A (en) * 2003-10-29 2007-08-02 リボメド バイオテクノロジーズ，インコーポレイテッド Compositions, methods, and detection techniques for iterative oligonucleotide synthesis
US20050153333A1 (en) * 2003-12-02 2005-07-14 Sooknanan Roy R. Selective terminal tagging of nucleic acids
EP1694871B1 (en) 2003-12-19 2010-08-25 Gen-Probe Incorporated Compositions, methods and kits for detecting the nucleic acids of hiv-1 and hiv-2
US20050147975A1 (en) * 2003-12-24 2005-07-07 Schembri Carol T. Methods and compositions for amplification of genomic DNA
US20050208538A1 (en) 2003-12-29 2005-09-22 Nurith Kurn Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
US7670771B2 (en) 2004-01-21 2010-03-02 University Of Utah Research Foundation Mutant sodium channel Nav1.7 nucleic acid methods
US20060003343A1 (en) 2004-01-23 2006-01-05 Biomerieux, Inc. Primer and probe design for efficient amplification and detection of HCV 3' non-translating region
US20050277121A1 (en) * 2004-06-11 2005-12-15 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
US7544792B2 (en) * 2004-07-13 2009-06-09 Gen-Probe Incorporated Compositions and methods for detection of hepatitis A virus nucleic acid
WO2006026388A2 (en) * 2004-08-27 2006-03-09 Gen-Probe Incorporated Single-primer nucleic acid amplification methods
US7713697B2 (en) * 2004-08-27 2010-05-11 Gen-Probe Incorporated Methods and kits for amplifying DNA
CA2847930C (en) 2004-09-30 2016-07-05 Gen-Probe Incorporated Assay for detecting and quantifying hiv-1
AU2005333163B2 (en) 2004-11-09 2011-06-16 Gen-Probe Incorporated Compositions and methods for detecting Group A streptococci
CA2491067A1 (en) 2004-12-24 2006-06-24 Stichting Katholieke Universiteit Mrna rations in urinary sediments and/or urine as a prognostic marker for prostate cancer
EP1861414B9 (en) 2005-02-07 2011-04-06 Gen-Probe Incorporated Compositions and methods for detecting group b streptococci
JP4819064B2 (en) 2005-02-18 2011-11-16 ジェン−プロウブ インコーポレイテッド Sample preparation method using alkali shock
EP1877418B1 (en) 2005-05-06 2013-11-20 Gen-Probe Incorporated Compositions and assays to detect influenza virus a nucleic acids
JP5187847B2 (en) 2005-05-06 2013-04-24 ジェン−プローブ・インコーポレーテッド Nucleic acid target capture method
US7550264B2 (en) * 2005-06-10 2009-06-23 Datascope Investment Corporation Methods and kits for sense RNA synthesis
US20070048741A1 (en) * 2005-08-24 2007-03-01 Getts Robert C Methods and kits for sense RNA synthesis
WO2007030759A2 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
WO2007047912A2 (en) 2005-10-17 2007-04-26 Gen-Probe Incorporated Compositions and methods to detect legionella pneumophila nucleic acid
CA2626977A1 (en) * 2005-10-27 2007-05-03 Rosetta Inpharmatics Llc Nucleic acid amplification using non-random primers
WO2007059179A1 (en) 2005-11-14 2007-05-24 Gen-Probe Incorporated Parametric calibration method
WO2007067907A1 (en) 2005-12-06 2007-06-14 Ambion, Inc. Reverse transcription primers and methods of design
JP5254040B2 (en) 2006-01-18 2013-08-07 アルゴス セラピューティクス，インコーポレイティド System and method for processing samples in a closed container and associated apparatus
RU2394915C2 (en) * 2006-03-24 2010-07-20 Александр Борисович Четверин Non-contact methods of detecting molecular colonies, sets of reagents and device for realising said methods
JP5154548B2 (en) * 2006-05-12 2013-02-27 ジェン−プローブ・インコーポレーテッド Compositions and methods for detecting enterococcal nucleic acids
US20100022403A1 (en) * 2006-06-30 2010-01-28 Nurith Kurn Methods for fragmentation and labeling of nucleic acids
JP5635772B2 (en) 2006-08-01 2014-12-03 ジェン−プロウブ インコーポレイテッド Method for capturing non-specific target of nucleic acid
US20080261216A1 (en) * 2006-09-08 2008-10-23 The Regents Of The University Of Michigan HERV Group II Viruses In Lymphoma And Cancer
US7709203B2 (en) * 2006-10-20 2010-05-04 E.I. Du Pont De Nemours And Company Sequences diagnostic for shrimp pathogens
EP2121956B1 (en) 2006-12-21 2016-08-17 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
US8183359B2 (en) * 2007-03-01 2012-05-22 Gen-Probe Incorporated Kits for amplifying DNA
WO2008108843A2 (en) * 2007-03-01 2008-09-12 Gen-Probe Incorporated Methods and kits for amplifying dna
US7964350B1 (en) 2007-05-18 2011-06-21 Applied Biosystems, Llc Sample preparation for in situ nucleic acid analysis
CA2692171C (en) 2007-06-22 2019-10-22 Randolph Watnick Methods and uses thereof of prosaposin
AU2008275978B9 (en) * 2007-07-18 2014-11-20 Gen-Probe Incorporated Compositions and methods to detect TMPRSS2/ERG transcript variants in prostate cancer
US8716190B2 (en) 2007-09-14 2014-05-06 Affymetrix, Inc. Amplification and analysis of selected targets on solid supports
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
WO2009086218A2 (en) * 2007-12-21 2009-07-09 Gen-Probe Incorporated Detection of antibiotic-resistant microorganisms
AU2008343380B2 (en) 2007-12-26 2012-07-12 Gen-Probe Incorporated Compositions and methods to detect Candida albicans nucleic acid
US20090203531A1 (en) 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
GB2470672B (en) 2008-03-21 2012-09-12 Nugen Technologies Inc Methods of RNA amplification in the presence of DNA
EP3392351A1 (en) 2008-04-21 2018-10-24 Gen-Probe Incorporated Method for detecting chikungunya virus
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
DK2644707T3 (en) 2008-04-30 2015-06-29 Integrated Dna Tech Inc RNase-H-based assays using modified RNA monomers.
WO2009140666A2 (en) 2008-05-15 2009-11-19 Ribomed Biotechnologies, Inc. METHODS AND REAGENTS FOR DETECTING CpG METHYLATION WITH A METHYL CpG BINDING PROTEIN (MBP)
US8211644B2 (en) * 2008-07-13 2012-07-03 Ribomed Biotechnologies, Inc. Molecular beacon-based methods for detection of targets using abscription
US8211637B2 (en) 2008-12-19 2012-07-03 Life Technologies Corporation Proteinase K inhibitors, methods and compositions therefor
AU2010217928B2 (en) 2009-02-26 2013-06-06 Gen-Probe Incorporated Assay for detection of human parvovirus nucleic acid
KR20120007002A (en) 2009-03-15 2012-01-19 리보메드 바이오테크놀로지스, 인코퍼레이티드 Abscription based molecular detection
AU2010264468B2 (en) 2009-06-23 2015-01-22 Gen-Probe Incorporated Compositions and methods for detecting nucleic acid from Mollicutes
CA2766391C (en) 2009-07-01 2021-04-20 Gen-Probe Incorporated Methods and compositions for multiplex nucleic acid amplification
US20110059453A1 (en) * 2009-08-23 2011-03-10 Affymetrix, Inc. Poly(A) Tail Length Measurement by PCR
WO2011060014A1 (en) 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
CA2780678A1 (en) 2009-11-19 2011-05-26 Ou Solis Biodyne Compositions for increasing polypeptide stability and activity, and related methods
US9121054B2 (en) * 2009-12-08 2015-09-01 Biohelix Corporation Detection of nucleic acid amplification products in the presence of an internal control sequence on an immunochromatographic strip
EP3925670A1 (en) 2009-12-17 2021-12-22 Children's Medical Center, Corp. Saposin-a derived peptides and uses thereof
WO2011091330A1 (en) 2010-01-22 2011-07-28 Gen-Probe Incorporated Probes for detecting the presence of trichomonas vaginalis in a sample
CN102725424B (en) 2010-01-25 2014-07-09 Rd生物科技公司 Self-folding amplification of target nucleic acid
EP2536849B1 (en) 2010-02-17 2016-05-11 Gen-Probe Incorporated Compositions and methods to detect atopobium vaginae nucleic acid
CA3011697C (en) 2010-04-21 2020-04-07 Gen-Probe Incorporated Compositions, methods and kits to detect herpes simplex virus nucleic acids
EP3674423A1 (en) 2010-07-12 2020-07-01 Gen-Probe Incorporated Compositions and assays to detect seasonal h3 influenza a virus nucleic acid
EP2752671A3 (en) 2010-07-23 2016-08-24 Beckman Coulter, Inc. System or method of including analytical units
EP2611932A2 (en) 2010-08-30 2013-07-10 Gen-Probe Incorporated Compositions, methods and reaction mixtures for the detection of xenotropic murine leukemia virus-related virus
JP2013545439A (en) 2010-09-17 2013-12-26 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ Functional genomics assay to characterize the usefulness and safety of pluripotent stem cells
EP2625297B1 (en) 2010-10-04 2018-10-10 Gen-Probe Prodesse, Inc. Compositions, methods and kits to detect adenovirus nucleic acids
EP2683833B1 (en) 2011-03-10 2018-09-26 Gen-Probe Incorporated Methods for the selection and optimization of oligonucleotide tag sequences
AU2012236896A1 (en) 2011-03-25 2013-05-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
US9657352B2 (en) 2011-04-25 2017-05-23 Gen-Probe Incorporated Compositions and methods for detecting BV-associated bacterial nucleic acid
AU2012275841A1 (en) 2011-06-27 2014-01-16 The Jackson Laboratory Methods and compositions for treatment of cancer and autoimmune disease
AU2012284307A1 (en) 2011-07-15 2013-05-09 Gen-Probe Incorporated Compositions and method for detecting human parvovirus nucleic acid and for detecting hepatitis A virus nucleic acids in single-plex or multiplex assays
JP2014526892A (en) * 2011-08-18 2014-10-09 ネステク ソシエテ アノニム Compositions and methods for detecting allelic polymorphisms
AU2012304327B2 (en) 2011-09-08 2015-07-09 Gen-Probe Incorporated Compositions and methods for detecting BV-associated bacterial nucleic acid
WO2013055911A1 (en) 2011-10-14 2013-04-18 Dana-Farber Cancer Institute, Inc. Znf365/zfp365 biomarker predictive of anti-cancer response
SG11201401628WA (en) 2011-10-19 2014-05-29 Nugen Technologies Inc Compositions and methods for directional nucleic acid amplification and sequencing
BR112014011035A2 (en) 2011-11-07 2017-06-13 Beckman Coulter, Inc. aliquot system and workflow
WO2013070756A2 (en) 2011-11-07 2013-05-16 Beckman Coulter, Inc. System and method for processing samples
US9046506B2 (en) 2011-11-07 2015-06-02 Beckman Coulter, Inc. Specimen container detection
WO2013070748A1 (en) 2011-11-07 2013-05-16 Beckman Coulter, Inc. Magnetic damping for specimen transport system
CN104105969B (en) 2011-11-07 2016-10-12 贝克曼考尔特公司 Centrifuge system and workflow
BR112014011048A2 (en) 2011-11-07 2017-05-02 Beckman Coulter Inc robotic arm
DE102011120550B4 (en) 2011-12-05 2013-11-07 Gen-Probe Prodesse, Inc. Compositions, methods and kits for the detection of adenovirus nucleic acids
US9824179B2 (en) 2011-12-09 2017-11-21 Adaptive Biotechnologies Corp. Diagnosis of lymphoid malignancies and minimal residual disease detection
WO2013096868A2 (en) 2011-12-22 2013-06-27 Children's Medical Center Corporation Saposin-a derived peptides and uses thereof
WO2013096798A2 (en) * 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Amplification of a sequence from a ribonucleic acid
CA2862552A1 (en) 2012-01-26 2013-08-01 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
WO2013126793A2 (en) 2012-02-24 2013-08-29 Gen-Probe Prodesse, Inc. Detection of shiga toxin genes in bacteria
AU2013205110B2 (en) 2012-04-24 2016-10-13 Gen-Probe Incorporated Compositions, Methods and Kits to Detect Herpes Simplex Virus Nucleic Acids
PL2831276T3 (en) 2012-05-08 2016-10-31 Compositions and method for measuring and calibrating amplification bias in multiplexed pcr reactions
AU2013205064B2 (en) 2012-06-04 2015-07-30 Gen-Probe Incorporated Compositions and Methods for Amplifying and Characterizing HCV Nucleic Acid
AU2013273987B2 (en) 2012-06-15 2018-08-09 Adaptive Biotechnologies Corporation Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set
JP6181751B2 (en) 2012-06-18 2017-08-16 ニューゲン テクノロジーズ， インコーポレイテッド Compositions and methods for negative selection of unwanted nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
AU2013205087B2 (en) 2012-07-13 2016-03-03 Gen-Probe Incorporated Method for detecting a minority genotype
EP2877952B1 (en) 2012-07-27 2023-04-12 Gen-Probe Incorporated Dual reference calibration method and system for quantifying polynucleotides
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
JP6464086B2 (en) 2012-08-30 2019-02-06 ジェン−プローブ・インコーポレーテッド Multiphase nucleic acid amplification
WO2014055561A1 (en) 2012-10-01 2014-04-10 Adaptive Biotechnologies Corporation Immunocompetence assessment by adaptive immune receptor diversity and clonality characterization
WO2014055746A1 (en) 2012-10-04 2014-04-10 The Board Of Trustees Of The Leland Stanford Junior University Methods and reagents for detection, quantitation, and serotyping of dengue viruses
AU2013205122B2 (en) 2012-10-11 2016-11-10 Gen-Probe Incorporated Compositions and Methods for Detecting Human Papillomavirus Nucleic Acid
AU2013205090B2 (en) 2012-12-07 2016-07-28 Gen-Probe Incorporated Compositions and Methods for Detecting Gastrointestinal Pathogen Nucleic Acid
US10077475B2 (en) 2013-01-24 2018-09-18 California Institute Of Technology FRET-based analytes detection and related methods and systems
JP2016509480A (en) 2013-01-24 2016-03-31 カリフォルニア インスティチュート オブ テクノロジー Chromophore-based characterization and detection methods
CA2905144C (en) 2013-03-14 2023-08-22 Lyle J. Arnold Methods for amplification of nucleic acids on solid support
JP6431895B2 (en) 2013-03-15 2018-12-05 アダプティブ バイオテクノロジーズ コーポレイション Reconstituted adaptive immune receptor genes uniquely tagged in a complex gene set
ES2795677T3 (en) 2013-03-15 2020-11-24 Gen Probe Inc Method, apparatus and software product for calibration
EP2971130A4 (en) 2013-03-15 2016-10-05 Nugen Technologies Inc Sequential sequencing
AU2014241191A1 (en) 2013-03-27 2015-10-22 Sample Technologies, Inc. Recombinant phage and bacterial detection methods
JP6697380B2 (en) 2013-06-10 2020-05-20 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ Early developmental genomic assay to characterize the utility and safety of pluripotent stem cells
JP2016521996A (en) 2013-06-19 2016-07-28 サンプルシックス テクノロジーズ，インコーポレイティド Phage-based bacterial detection assay
EP3011334A1 (en) 2013-06-20 2016-04-27 The Trustees Of The University Of Pennsylvania Methods for diagnosing pancreatic cancer
AU2014306512C1 (en) 2013-08-14 2021-07-08 Gen-Probe Incorporated Compositions and methods for detecting HEV nucleic acid
US9546399B2 (en) 2013-11-13 2017-01-17 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
US9243289B2 (en) * 2013-12-23 2016-01-26 Roche Molecular Systems, Inc. Method for screening reagents used in PCR assays
DE102015017080B3 (en) 2014-02-28 2024-01-04 Gen-Probe Incorporated Method for isolating nucleic acid from cytological samples in liquid preservatives containing formaldehyde
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
AU2014384731B2 (en) 2014-02-28 2017-12-14 Gen-Probe Incorporated Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde
AU2015249374A1 (en) 2014-04-24 2016-12-01 Dana-Farber Cancer Institute, Inc. Tumor suppressor and oncogene biomarkers predictive of anti-immune checkpoint inhibitor response
WO2016011383A1 (en) 2014-07-17 2016-01-21 The Trustees Of The University Of Pennsylvania Methods for using exosomes to monitor transplanted organ status
WO2016057367A1 (en) 2014-10-06 2016-04-14 Dana-Farber Cancer Institute, Inc. Angiopoietin-2 biomarkers predictive of anti-immune checkpoint response
CN113230384A (en) 2014-10-09 2021-08-10 丹娜法伯癌症研究院 Multiple-variable IL-2 dosage regimen for treating immune disorders
AU2015336079C1 (en) 2014-10-20 2020-12-17 Gen-Probe Incorporated Red blood cell lysis solution
US10865433B2 (en) 2015-01-09 2020-12-15 Gen-Probe Incorporated Reaction mixtures for diagnosing bacterial vaginosis
CA2977532A1 (en) 2015-03-06 2016-09-15 Dana-Farber Cancer Institute, Inc. Pd-l2 biomarkers predictive of pd-1 pathway inhibitor responses in esophagogastric cancers
EP3910075A1 (en) 2015-03-16 2021-11-17 Gen-Probe Incorporated Kits for detecting bacterial nucleic acid and diagnosing bacterial vaginosis
JP6894439B2 (en) 2016-01-04 2021-06-30 ジェン−プローブ・インコーポレーテッド Methods and Compositions for Detecting Candida Species
DE202017007130U1 (en) 2016-04-27 2019-08-29 Gen-Probe Inc. Lysis reagent for blood cells
US10508312B2 (en) 2016-06-10 2019-12-17 Gen-Probe Incorporated Compositions and methods for detecting Zika virus nucleic acid
JP7466308B2 (en) 2016-09-20 2024-04-12 デイナ ファーバー キャンサー インスティチュート，インコーポレイテッド Compositions and methods for identifying, evaluating, preventing, and treating AML using USP10 biomarkers and modulators - Patents.com
EP3529381B1 (en) 2016-10-19 2022-07-06 Gen-Probe Incorporated Compositions and methods for detecting or quantifying hepatitis c virus
EP3541959A1 (en) 2016-11-21 2019-09-25 Gen-Probe Incorporated Compositions and methods for detecting or quantifying hepatitis b virus
US10427162B2 (en) 2016-12-21 2019-10-01 Quandx Inc. Systems and methods for molecular diagnostics
GB201703383D0 (en) 2017-03-02 2017-04-19 Gargle Tech Ltd Testing for particulates
US10844425B2 (en) 2017-03-24 2020-11-24 Gen-Probe Incorporated Compositions and methods for detecting or quantifying parainfluenza virus
CA3176529C (en) 2017-03-24 2024-03-05 Gen-Probe Incorporated Compositions and methods for detection of viral pathogens in samples
EP3634496A4 (en) 2017-06-06 2021-09-08 Dana-Farber Cancer Institute, Inc. Methods for sensitizing cancer cells to t cell-mediated killing by modulating molecular pathways
AU2018281196B2 (en) 2017-06-07 2022-04-28 Gen-Probe Incorporated Detecting Babesia species nucleic acid in a sample
EP4289507A3 (en) 2017-07-10 2024-02-28 Gen-Probe Incorporated Analytical systems and methods for nucleic acid amplification using sample assigning parameters
EP4219766A3 (en) 2017-08-11 2023-10-11 Gen-Probe Incorporated Compositions and methods for detecting staphylococcus aureus
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
CN111465707A (en) 2017-11-17 2020-07-28 简·探针公司 Compositions and methods for detecting C1orf43 nucleic acids
WO2019118735A1 (en) 2017-12-15 2019-06-20 Gen-Probe Incorporated Compositions and methods for detecting toxigenic clostridium difficile
CN111902213B (en) 2018-01-29 2022-12-06 简·探针公司 Analysis system and method
GB2597569B (en) 2018-06-13 2023-01-25 Gen Probe Inc Compositions and methods for detecting group B streptococcus nucleic acid
JP7470095B2 (en) 2018-07-10 2024-04-17 ジェン－プローブ・インコーポレーテッド Methods and systems for detecting and quantifying nucleic acids - Patents.com
EP3830302B1 (en) 2018-08-01 2022-10-05 Gen-Probe Incorporated Compositions and methods for detecting nucleic acids of epstein-barr virus
JP2021532808A (en) 2018-08-08 2021-12-02 ジェン−プローブ・インコーポレーテッド Compositions, Methods, and Kits for Detecting MYCOPLASMA GENITALIUM
CA3107043A1 (en) 2018-08-21 2020-02-27 Gen-Probe Incorporated Compositions and methods for amplifying, detecting or quantifying human cytomegalovirus
AU2019324196A1 (en) 2018-08-24 2021-03-18 Gen-Probe Incorporated Compositions and methods for detecting bacterial nucleic acid and diagnosing bacterial vaginosis
EP4205851A3 (en) 2018-09-05 2023-10-04 Hero Scientific Ltd Testing for particulates
WO2020069085A2 (en) 2018-09-27 2020-04-02 Gen-Probe Incorporated Compositions and methods for detecting bordetella pertussis and bordetella parapertussis nucleic acid
WO2020086546A1 (en) 2018-10-22 2020-04-30 Gen-Probe Incorporated Compositions and methods for amplifying, detecting or quantifying human polyomavirus bk virus
GB2595076B8 (en) * 2018-11-21 2023-08-23 Agilent Technologies Inc Methods for targeted nucleic acid library formation
TW202030333A (en) 2018-12-20 2020-08-16 美商簡 探針公司 Compositions and methods for detecting plasmodium species nucleic acid
US20220080425A1 (en) 2018-12-31 2022-03-17 Gen-Probe Incorporated Tray for transfering solid reagents to a multi-well cartridge
WO2020197987A1 (en) 2019-03-22 2020-10-01 Gen-Probe Incorporated Compositions and methods for detecting group a streptococcus
US20220349910A1 (en) 2019-05-03 2022-11-03 Gen-Probe Incorporated Receptacle delivery system
US20220307093A1 (en) 2019-07-03 2022-09-29 Gen-Probe Incorporated Oligonucleotides for use in determining the presence of trichomonas vaginalis in a sample
CA3152309A1 (en) 2019-08-23 2021-03-04 Gen-Probe Incorporated Compositions, methods and kits for detecting treponema pallidum
EP4025715A1 (en) 2019-09-05 2022-07-13 Gen-Probe Incorporated Detection of chlamydia trachomatis nucleic acid variants
EP4073268B1 (en) 2019-12-09 2024-04-10 Gen-Probe Incorporated Quantification of polynucleotide analytes from dried samples
CN115349019A (en) 2020-01-16 2022-11-15 Dnae诊断有限公司 Compositions, kits and methods for isolating target polynucleotides
JP2023516683A (en) 2020-03-04 2023-04-20 ジェン－プローブ・インコーポレーテッド Compositions and methods for detecting SARS-CoV-2 nucleic acids
US20230220499A1 (en) 2020-05-07 2023-07-13 Grifols Diagnostic Solutions Inc. Methods and compositions for detecting sars-cov-2 nucleic acid
AU2021308095A1 (en) 2020-07-17 2023-03-09 Gen-Probe Incorporated Detection of macrolide-resistant mycoplasma genitalium
EP3978626A1 (en) * 2020-10-02 2022-04-06 Lexogen GmbH Method and means for generating transcribed nucleic acids
AU2021354916A1 (en) 2020-10-02 2023-04-06 Lexogen Gmbh Method and means for generating transcribed nucleic acids
WO2022087257A2 (en) 2020-10-21 2022-04-28 Gen-Probe Incorporated Fluid container management system
CN116648253A (en) 2020-10-29 2023-08-25 伊夫罗生物科学公司 Composition comprising spirulina component
WO2022149135A2 (en) 2021-01-06 2022-07-14 Hero Scientific Ltd. Filtration sampling devices
AU2022229805A1 (en) 2021-03-02 2023-09-21 Dana-Farber Cancer Institute, Inc. Methods of treating red blood cell disorders
AU2022273052A1 (en) 2021-05-14 2023-11-30 Gen-Probe Incorporated Compositions and methods for detecting human adenovirus nucleic acid
WO2022251166A2 (en) 2021-05-25 2022-12-01 Evelo Biosciences, Inc. Bacterial compositions comprising soy hemoglobin
WO2022261183A2 (en) 2021-06-08 2022-12-15 Dana-Farber Cancer Institute, Inc. Compositions and methods for treating and/or identifying an agent for treating intestinal cancers
WO2023278529A1 (en) 2021-07-01 2023-01-05 Gen-Probe Incorporated Enzyme formulations and reaction mixtures for nucleic acid amplification
WO2023002203A1 (en) 2021-07-21 2023-01-26 Dnae Diagnostics Limited Method and system comprising a cartridge for sequencing target polynucleotides
GB202110479D0 (en) 2021-07-21 2021-09-01 Dnae Diagnostics Ltd Compositions, kits and methods for sequencing target polynucleotides
GB202110485D0 (en) 2021-07-21 2021-09-01 Dnae Diagnostics Ltd Compositions, kits and methods for sequencing target polynucleotides
AU2022319876A1 (en) 2021-07-27 2024-01-18 Gen-Probe Incorporated Compositions and methods for detecting gastrointestinal pathogens
WO2023097119A2 (en) 2021-11-29 2023-06-01 Dana-Farber Cancer Institute, Inc. Methods and compositions to modulate riok2
EP4282980A1 (en) 2022-05-23 2023-11-29 Mobidiag Oy Methods for amplifying a nucleic acid
WO2024054924A1 (en) 2022-09-08 2024-03-14 Gen-Probe Incorporated Method of detecting nucleic acid analytes using dual-specificity primers

Family Cites Families (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA559709A (en) * 1958-07-01 International Standard Electric Corporation Travelling wave tubes
US4876197A (en) * 1983-02-22 1989-10-24 Chiron Corporation Eukaryotic regulatable transcription
US4957858A (en) * 1986-04-16 1990-09-18 The Salk Instute For Biological Studies Replicative RNA reporter systems
US4786600A (en) * 1984-05-25 1988-11-22 The Trustees Of Columbia University In The City Of New York Autocatalytic replication of recombinant RNA
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
DK171161B1 (en) * 1985-03-28 1996-07-08 Hoffmann La Roche A method for detecting the presence or absence of at least one specific nucleic acid sequence in a sample or for distinguishing two different nucleic acid sequences in this sample
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
AU606043B2 (en) * 1985-03-28 1991-01-31 F. Hoffmann-La Roche Ag Detection of viruses by amplification and hybridization
US4876187A (en) * 1985-12-05 1989-10-24 Meiogenics, Inc. Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
WO1988003957A1 (en) * 1986-11-24 1988-06-02 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
IL86724A (en) * 1987-06-19 1995-01-24 Siska Diagnostics Inc Method and kits for the amplification and detection of nucleic acid sequences
US4994368A (en) * 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
WO1989001050A1 (en) * 1987-07-31 1989-02-09 The Board Of Trustees Of The Leland Stanford Junior University Selective amplification of target polynucleotide sequences
KR960002561B1 (en) * 1987-09-21 1996-02-22 젠-프로우브 인코퍼레이티드 Homogeneous protection assays
CA1339303C (en) * 1987-09-21 1997-08-19 Lyle John Arnold Jr. Non-nucleotide linking reagents for nucleotide probes
US5030557A (en) * 1987-11-24 1991-07-09 Ml Technology Venture Means and method for enhancing nucleic acid hybridization
CA1323293C (en) * 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
AU624601B2 (en) * 1988-01-21 1992-06-18 Genentech Inc. Amplification and detection of nucleic acid sequences
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5130238A (en) * 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
BR8907830A (en) * 1988-12-16 1991-10-22 Siska Diagnostics Inc PROCESS FOR PREPARING A DOUBLE-FILAMENT DNA THAT CODES A SEQUENCE THAT MATCHES A OBJECTIVE NUCLEIC ACID SEQUENCE AND PROCESS USED FOR DETECTION OF AT LEAST ONE SPECIFIC OBJECTIVE NUCLEIC ACID SEQUENCE
IE66597B1 (en) * 1989-05-10 1996-01-24 Akzo Nv Method for the synthesis of ribonucleic acid (RNA)
CA2016553A1 (en) * 1989-05-16 1990-11-16 Dyann F. Wirth Dna hybridization probes for identification of mycobacteria
US5112734A (en) * 1989-05-26 1992-05-12 Gene-Trak Systems Target-dependent synthesis of an artificial gene for the synthesis of a replicatable rna
EP0731175A3 (en) * 1989-07-11 2004-05-26 Gen-Probe Incorporated Oligonucleotide probes for HIV nucleic acid
FR2659086B1 (en) * 1990-03-02 1992-06-12 Pasteur Institut NUCLEOTIDE FRAGMENTS CHARACTERISTICS OF DNA FRAGMENTS OF MYCOBACTERIA. THEIR USE AS A PRIMER FOR THE DETECTION OF AN INFECTION DUE TO MYCOBACTERIA.
FR2651505B1 (en) * 1989-09-06 1994-07-22 Pasteur Institut FRAGMENTS OF NUCLEIC ACIDS DERIVED FROM AN APPROPRIATE MYCOBACTERIA GENOME, THEIR APPLICATIONS IN THE DIAGNOSIS OF MYCOBACTERIA AND PLASMID INFECTIONS CONTAINING SAID FRAGMENTS.
WO1991004340A1 (en) * 1989-09-20 1991-04-04 Cambridge Biotech Corporation In vitro, isothermal nucleic acid amplification
US5215899A (en) * 1989-11-09 1993-06-01 Miles Inc. Nucleic acid amplification employing ligatable hairpin probe and transcription
WO1991010746A1 (en) * 1990-01-10 1991-07-25 Chiron Corporation Dna-dependent rna polymerase transcripts as reporter molecules for signal amplification in nucleic acid hybridization assays
CA2033718A1 (en) * 1990-01-19 1991-07-20 Ronald M. Atlas Process for detection of water-borne microbial pathogens and indicators of human fecal contamination in water samples and kits therefor
WO1991015601A1 (en) * 1990-04-05 1991-10-17 The United States Of America, As Represented By The Secretary, U.S. Department Of Commerce Modified rna template-specific polymerase chain reaction
CH681075A5 (en) * 1990-06-08 1993-01-15 Sigg Aluminium & Metallwaren
FR2663033B1 (en) * 1990-06-08 1992-09-04 Pasteur Institut SPECIFIC DETECTION OF MYCOBACTERIUM TUBERCULOSIS.

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DE69333891T2 (en) 2006-07-27
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JP4137996B2 (en) 2008-08-20
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TW360712B (en) 1999-06-11
ES2250960T3 (en) 2006-04-16
WO1993022461A1 (en) 1993-11-11
EP0587266A1 (en) 1994-03-16
AU4222493A (en) 1993-11-29
DK0587266T3 (en) 2005-12-05
EP0587266B1 (en) 2005-10-26
AU681082B2 (en) 1997-08-21
DE69333891D1 (en) 2005-12-01

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