CA2144328A1 - Improved method for detection of specific target cells in specialized or mixed cell population and solutions containing mixed cell populations - Google Patents
Improved method for detection of specific target cells in specialized or mixed cell population and solutions containing mixed cell populationsInfo
- Publication number
- CA2144328A1 CA2144328A1 CA002144328A CA2144328A CA2144328A1 CA 2144328 A1 CA2144328 A1 CA 2144328A1 CA 002144328 A CA002144328 A CA 002144328A CA 2144328 A CA2144328 A CA 2144328A CA 2144328 A1 CA2144328 A1 CA 2144328A1
- Authority
- CA
- Canada
- Prior art keywords
- cells
- target
- antibodies
- cell
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57407—Specifically defined cancers
- G01N33/57415—Specifically defined cancers of breast
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/961—Chemistry: molecular biology and microbiology including a step of forming, releasing, or exposing the antigen or forming the hapten-immunogenic carrier complex or the antigen per se
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/824—Immunological separation techniques
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/828—Protein A
Abstract
The invention relates to a method for detecting specific target-cells in a simple and time saving way, using paramagnetic particles, antibodies recognizing the Fc portions of target-cell associating antibodies and target-cell associating antibodies direct-ed to specific antigen determinants in the target-cell membranes.
Incubation of the cell suspension with a mild detergent and/or second set of antibodies or antibody fragments, prelabeled or not with fluorescent agents, metallocolloids, radioisotopes, biotin--complexes or certain enzymes allowing visualization, will dramatically increase the specificity of the method. The method can fur-ther be used for isolation of the target-cells by magnetic field application and a kit for performing the method according to the invention is described.
Incubation of the cell suspension with a mild detergent and/or second set of antibodies or antibody fragments, prelabeled or not with fluorescent agents, metallocolloids, radioisotopes, biotin--complexes or certain enzymes allowing visualization, will dramatically increase the specificity of the method. The method can fur-ther be used for isolation of the target-cells by magnetic field application and a kit for performing the method according to the invention is described.
Claims (16)
1. A method for detecting specific target cells in cell suspensions of mixed cell populations and in fluid systems containing mixed cell populations, and in single cell suspen-sions prepared from solid tissues, characterized by comprising the following steps:
1.1. coating , by 2 per ce known procedure, paramagnetic particles or beads with either, a) antibodies, or antibody fragments directed against membrane structures specifically expressed on target-cells and not on non-target-cells in the cell mixture or;
b) antibodies, preferably polyclonal anti-mouse or monoclonal rat anti-mouse antibodies or anti-human antibodies, capable of binding to the Fc-portions of the said antibodies, directed against the membrane structures; and 1.2.1. mixing the target-cell-associating antibodies (murine or human) which is attached to the said particles or beads, or attached to the beads pre-coated with anti-mouse or antihuman antibodies recognizing the Fc-portions of the target-associating antibodies, with the cell suspension containing the target-cells, or, 1.2.2. mixing free target-cell-associating antibodies with the cell suspension containing the target cells and incubate this mixture for 5-10 min to 2 h, preferably 30 min, at a temperature between 0°C and 20°C, preferably 4°C under gentle rotation, and;
1.3. incubating the mixture of the cell suspension and target-associating antibodies attached to paramagnetic particles or beads (1.2.1), or paramagnetic particles or beads, precoated with anti-mouse or anti-human antibodies recognizing the Fc-portion of the target-associating antibodies, to the mixture of incubated free target associating antibody and cell suspension (1.2.2.), and incubating, for 5-10 min to 2 h, preferably 30 min, at a temperature between 0°C and 25°C, preferably 4°C, under gentle rotation, and;
1.4.1. if the target cell population is contained in blood or bone marrow aspirates the hydrophobic forces associated with antibody-coated particles are reduced by pre-incubating the antibody-coated particles and the cell suspension with mild detergents in suitable concentrations, e.g. Tween 20(TM) in concentrations less than 0.1% for 30 min at 4°C, and/or 1.4.2. by incubating the cell suspensions, untreated or pretreated with formalin, alcohol or other fixatives, with other antibodies or antibody fragments binding to extracellular or intracellular molecules present in the target cells and the antibodies used are labeled in advance by peroxidase, alkaline phosphatase, or other enzymes permitting visualization of the binding by addition and incubation with relevant substrates, or 1.4.3. the antibody fragments are biotinylated and the binding visualized when adding the incubating with avidin complexed to peroxidase, alkaline phosphatase, or other enzymes, with addition and incubation with relevant substrates, or 1.5.1. subjecting the incubated paramagnetic particle-antibodies-cell mixture (1.3) to a magnetic field if the density of target-cells is low, or if the ratio of target cell/total cells in the cell mixture is low (? 1 %) and then examining and counting the stained or unstained particle-target-cell complexes in the cell suspension, using a micropscope and/or a suitable cell/particle counting device, or, 1.5.2. examining and counting the target-cells in the incubated mixture of paramagnetic particles, antibodies and cell mixture (1.3), or in the case when the antibodies or antibody fragments are conjugated to non-paramagnetic particles that can be visualized directly because of colour or through enzymatic activation, using a microscope and/or a suitable cell/particle counting device if the ratio of target-cells/total cells in the cell suspension is adequate (> 1 %).
1.1. coating , by 2 per ce known procedure, paramagnetic particles or beads with either, a) antibodies, or antibody fragments directed against membrane structures specifically expressed on target-cells and not on non-target-cells in the cell mixture or;
b) antibodies, preferably polyclonal anti-mouse or monoclonal rat anti-mouse antibodies or anti-human antibodies, capable of binding to the Fc-portions of the said antibodies, directed against the membrane structures; and 1.2.1. mixing the target-cell-associating antibodies (murine or human) which is attached to the said particles or beads, or attached to the beads pre-coated with anti-mouse or antihuman antibodies recognizing the Fc-portions of the target-associating antibodies, with the cell suspension containing the target-cells, or, 1.2.2. mixing free target-cell-associating antibodies with the cell suspension containing the target cells and incubate this mixture for 5-10 min to 2 h, preferably 30 min, at a temperature between 0°C and 20°C, preferably 4°C under gentle rotation, and;
1.3. incubating the mixture of the cell suspension and target-associating antibodies attached to paramagnetic particles or beads (1.2.1), or paramagnetic particles or beads, precoated with anti-mouse or anti-human antibodies recognizing the Fc-portion of the target-associating antibodies, to the mixture of incubated free target associating antibody and cell suspension (1.2.2.), and incubating, for 5-10 min to 2 h, preferably 30 min, at a temperature between 0°C and 25°C, preferably 4°C, under gentle rotation, and;
1.4.1. if the target cell population is contained in blood or bone marrow aspirates the hydrophobic forces associated with antibody-coated particles are reduced by pre-incubating the antibody-coated particles and the cell suspension with mild detergents in suitable concentrations, e.g. Tween 20(TM) in concentrations less than 0.1% for 30 min at 4°C, and/or 1.4.2. by incubating the cell suspensions, untreated or pretreated with formalin, alcohol or other fixatives, with other antibodies or antibody fragments binding to extracellular or intracellular molecules present in the target cells and the antibodies used are labeled in advance by peroxidase, alkaline phosphatase, or other enzymes permitting visualization of the binding by addition and incubation with relevant substrates, or 1.4.3. the antibody fragments are biotinylated and the binding visualized when adding the incubating with avidin complexed to peroxidase, alkaline phosphatase, or other enzymes, with addition and incubation with relevant substrates, or 1.5.1. subjecting the incubated paramagnetic particle-antibodies-cell mixture (1.3) to a magnetic field if the density of target-cells is low, or if the ratio of target cell/total cells in the cell mixture is low (? 1 %) and then examining and counting the stained or unstained particle-target-cell complexes in the cell suspension, using a micropscope and/or a suitable cell/particle counting device, or, 1.5.2. examining and counting the target-cells in the incubated mixture of paramagnetic particles, antibodies and cell mixture (1.3), or in the case when the antibodies or antibody fragments are conjugated to non-paramagnetic particles that can be visualized directly because of colour or through enzymatic activation, using a microscope and/or a suitable cell/particle counting device if the ratio of target-cells/total cells in the cell suspension is adequate (> 1 %).
2. Method according to claim 1, characterized by directing the antibody or fragments thereof against the antigens in normal, living cells, such as liver hepatocytes, Kupffer cells and endothelial cells type 1 and 2 and Clara cells of the lung, endothelial cells of specific organs, pancreatic exocrine and endocrine cells, kidney tubule cells, bladder epithelial cells, brain glial and ependymal cells, bladder and prostate epithelial cells, ciliated cells of airways, different subpopulations of mucosal cells in the gastrointestinal tract, pituitary cells, and other endocrine cells in various hormone-producing organs.
3. Method according to one of the preceding claims, characterized by using as the said target-cell antibody an antibody which is reactive with antigens present on subpopulations of normal cells and oncogenic products expressed on the membrane of normal tissue cells.
4. Method according to one of the preceding claims, characterized by using as the said positive selecting antibody, an antibody which is directed against growth factor receptors on the membrane of normal cells, for example the EGF-receptor, PDGF (A and B) receptor, insuline receptors, insuline-like receptors transferrin receptor, NGF and FGF receptors.
5. Method according to one of the preceding claims, characterized by using an antibody directed against the group of integrins and other adhesion membrane molecules, and MDR
proteins in normal cells.
proteins in normal cells.
6. Method according to one of the preceding claims, characterized by directing the antibody or fragments thereof against antigen or receptors in cells with abnormal developmental patterns, preferably such as primary and metastatic cancer cells.
7. Method according to one of the preceding claims, characterized by using as the said target-cell associating antibodies, antibodies of the IgG isotype, or F(ab')2 or F(ab) fragments, or IgM, or fragments of IgM.
8. Method according to one of the preceding claims, characterized by preparing the mentioned cell suspension from mixed cell populations comprising mammalian tissues, for examples human bone marrow and peripheral blood, from pleural and peritoneal effusions, other body fluids, for example urine, cerebrospinal fluid, semen, lymph, or from solid tumors in normal tissues and organs, for example liver, lymphatic nodes, spleen, lung, pancreas, bone tissue, central nervous system, prostatic gland, skin and mucous membranes.
9. Method according to one of the preceding claims, characterized by that the antibody or antibody fragments is directed against groups of antigen determinants, such as those listed in the Table 1 of the specification.
10. Method according to one of the preceding claims, characterized by using as the said target-cell antibody an antibody or antibody fragment which is directed against growth factor receptors and oncogene products expressed on the membrane of malignant cells, for example insuline receptors, insuline-like receptors and FGF receptors in addition to those listed in Table 1 of the specification.
11. Method according to one of the preceding claims, characterized by using an antibody or antibody fragment directed against the group of integrins, other adhesion membrane molecules and MDR proteins in abnormal cells as listed in Table 1.
12. Method according to one of the preceding claims, characterized in that the used antibodies, antibody fragments or combinations of these are directed to the antigen determinants as listed in Table 1 of the specification.
13. Method according to one of the preceding claims, characterized by using as the said antibody an antibody which is reactive with antigens present on abnormal cells, for example breast, ovarian and lung carcinoma cells, melanoma, sarcoma, glioblastoma and cancer cells of the gastrointestinal and genitourinary tract, and of the reticuloendothelial system, and/or target-cells associated with non-neoplastic diseases, such as cardiovascular, neurological, pulmonary, autoimmune gastrointestinal, genitourinary, reticuloendothelial and other disorders.
14. Use of the detection method according to one of the preceding claims, for isolation of target-cells, whereby the complex of cells and the paramagnetic particles are exposed to a magnetic field and the resulting magnetically aggregated cells are further subjected to biological, biochemical and immunological examinations, including also characterisation of specific genes at the DNA, mRNA and protein level, including polymerase chain reaction (PCR) and reverse transcriptase PCR.
15. Use of the method for detection of specific target-cells according to one of the preceding claims, whereby it is established in vitro cell cultures from the separated paramagnetic particle-target-cell-complexes, and/or for inoculation into immunodeficient animals, preferably to establish human tumor xenografts in the said animals.
16. Kit for performing the method according to one of the preceding claims, characterized by that it comprises;
1, specific antibodies or antibody fragments directed to the antigen receptors on the wanted target-cells, where said antibody or antibody fragment is bound or can be bound to included paramagnetic particles, without removing their antigen-binding ability, and/or 2, paramagnetic particles precoated with specific anti-Fc antibodies, preferably polyclonal anti-mouse, or monoclonal rat anti-mouse, or anti-human antibodies, capable of binding to the Fc portions of the target-cell associating antibodies, and specific free target-cell antibodies, and/or 3, paramagnetic particles precoated with specific anti-Fc antibodies, preferably polyclonal anti-mouse, or monoclonal rat anti-mouse, or anti-human antibodies, capable of binding to the Fc-portions of the target-cell associating antibodies, bound to specific anti-target-cell antibodies, and/or 4, other specific antibodies or antibody fragments directed against antigens/receptors within or on the wanted target cells, where said antibodies or antibody fragments are conjugated to biotin, peroxidase, alkaline phosphatase, or other enzymes, or where said antibodies or antibody fragments are bound to non-paramagnetic particles with specific colours or with bound enzymes such as peroxidase and alkaline phosphatase.
1, specific antibodies or antibody fragments directed to the antigen receptors on the wanted target-cells, where said antibody or antibody fragment is bound or can be bound to included paramagnetic particles, without removing their antigen-binding ability, and/or 2, paramagnetic particles precoated with specific anti-Fc antibodies, preferably polyclonal anti-mouse, or monoclonal rat anti-mouse, or anti-human antibodies, capable of binding to the Fc portions of the target-cell associating antibodies, and specific free target-cell antibodies, and/or 3, paramagnetic particles precoated with specific anti-Fc antibodies, preferably polyclonal anti-mouse, or monoclonal rat anti-mouse, or anti-human antibodies, capable of binding to the Fc-portions of the target-cell associating antibodies, bound to specific anti-target-cell antibodies, and/or 4, other specific antibodies or antibody fragments directed against antigens/receptors within or on the wanted target cells, where said antibodies or antibody fragments are conjugated to biotin, peroxidase, alkaline phosphatase, or other enzymes, or where said antibodies or antibody fragments are bound to non-paramagnetic particles with specific colours or with bound enzymes such as peroxidase and alkaline phosphatase.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| WOPCT/NO92/00151 | 1992-09-14 | ||
| PCT/NO1992/000151 WO1994007138A1 (en) | 1992-09-14 | 1992-09-14 | Detection of specific target cells in specialized or mixed cell population and solutions containing mixed cell populations |
| PCT/NO1993/000136 WO1994007139A1 (en) | 1992-09-14 | 1993-09-10 | Improved method for detection of specific target cells in specialized or mixed cell population and solutions containing mixed cell populations |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2144328A1 true CA2144328A1 (en) | 1994-03-31 |
| CA2144328C CA2144328C (en) | 2010-11-30 |
Family
ID=19907687
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2144328A Expired - Lifetime CA2144328C (en) | 1992-09-14 | 1993-09-10 | Improved method for detection of specific target cells in specialized or mixed cell population and solutions containing mixed cell populations |
Country Status (17)
| Country | Link |
|---|---|
| US (3) | US6893881B1 (en) |
| EP (1) | EP0660930B1 (en) |
| JP (1) | JP3417563B2 (en) |
| AT (1) | ATE186402T1 (en) |
| AU (2) | AU2593192A (en) |
| CA (1) | CA2144328C (en) |
| CZ (1) | CZ65995A3 (en) |
| DE (1) | DE69326956T2 (en) |
| DK (1) | DK0660930T3 (en) |
| ES (1) | ES2141170T3 (en) |
| FI (1) | FI951161A (en) |
| GR (1) | GR3032547T3 (en) |
| HU (1) | HU221234B1 (en) |
| PL (1) | PL177136B1 (en) |
| PT (1) | PT660930E (en) |
| SK (1) | SK281566B6 (en) |
| WO (2) | WO1994007138A1 (en) |
Families Citing this family (92)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2593192A (en) | 1992-09-14 | 1994-04-12 | Oystein Fodstad | Detection of specific target cells in specialized or mixed cell population and solutions containing mixed cell populations |
| NO180658C (en) | 1994-03-10 | 1997-05-21 | Oeystein Fodstad | Method and Device for Detecting Specific Target Cells in Specialized or Mixed Cell Populations and Solutions Containing Mixed Cell Populations |
| NO180167C (en) | 1994-09-08 | 1997-02-26 | Photocure As | Photochemical method for introducing molecules into the cytosol of cells |
| NO961031D0 (en) | 1996-03-13 | 1996-03-13 | Det Norske Radiumshospital Tum | Procedure for killing unwanted target cells |
| NO961221D0 (en) * | 1996-03-26 | 1996-03-26 | Oeystein Fodstad | Method of identifying new genes associated with site-preferential cancer metastasis formation |
| NO308755B1 (en) * | 1996-12-20 | 2000-10-23 | Iystein Fodstad | Method for characterizing abnormal cells, use and kits for carrying out the method |
| US6418338B1 (en) * | 1998-02-06 | 2002-07-09 | Phylatron Ltd. | Method for detecting and surgically removing lymphoid tissue involved in tumor progression |
| US6824995B1 (en) * | 1998-03-03 | 2004-11-30 | Emory University | Diagnostic for metastatic prostate cancer |
| DE19857618A1 (en) * | 1998-12-14 | 2000-06-21 | Georg S Wengler | Methods of locating foci of disease |
| US6828157B1 (en) * | 1999-05-04 | 2004-12-07 | Dan A. Pankowsky | Products and methods for single parameter and multiparameter phenotyping of cells |
| US6682940B2 (en) * | 1999-05-04 | 2004-01-27 | Dan A. Pankowsky | Products and methods for single parameter and multiparameter phenotyping of cells |
| US6692952B1 (en) * | 1999-11-10 | 2004-02-17 | Massachusetts Institute Of Technology | Cell analysis and sorting apparatus for manipulation of cells |
| US7572631B2 (en) * | 2000-02-24 | 2009-08-11 | Invitrogen Corporation | Activation and expansion of T cells |
| US20030235908A1 (en) * | 2000-02-24 | 2003-12-25 | Xcyte Therapies, Inc. | Activation and expansion of cells |
| US20030119185A1 (en) * | 2000-02-24 | 2003-06-26 | Xcyte Therapies, Inc. | Activation and expansion of cells |
| US6797514B2 (en) * | 2000-02-24 | 2004-09-28 | Xcyte Therapies, Inc. | Simultaneous stimulation and concentration of cells |
| US7541184B2 (en) * | 2000-02-24 | 2009-06-02 | Invitrogen Corporation | Activation and expansion of cells |
| AR028039A1 (en) * | 2000-05-03 | 2003-04-23 | Oncolytics Biotech Inc | REMOVAL OF REOVIRUS FROM NEOPLASTIC CELLS INTERMEDIATE BY RAS FROM MIXED CELL COMPOSITIONS |
| US20020019048A1 (en) * | 2000-05-25 | 2002-02-14 | Ronald Berenson | Methods for restoring or enhancing T-cell immune surveillance following naturally or artificially induced immunosuppression |
| FR2810045B1 (en) * | 2000-06-07 | 2004-09-03 | Assist Publ Hopitaux De Paris | METHOD FOR OBTAINING CELLULAR CELLULAR POPULATIONS OF MUSCLE ORIGIN AND USES THEREOF |
| US6913697B2 (en) | 2001-02-14 | 2005-07-05 | Science & Technology Corporation @ Unm | Nanostructured separation and analysis devices for biological membranes |
| US7169578B2 (en) * | 2001-07-27 | 2007-01-30 | Surface Logix, Inc. | Cell isolation and screening device and method of using same |
| US7169577B2 (en) * | 2001-07-27 | 2007-01-30 | Surface Logix, Inc. | Cell isolation and screening device and method of using same |
| US7285412B2 (en) * | 2001-07-27 | 2007-10-23 | Surface Logix Inc. | Device for magnetic immobilization of cells |
| EP1409746A2 (en) * | 2001-09-06 | 2004-04-21 | Adnagen AG | Method and kit for diagnosing or controlling the treatment of breast cancer |
| CA2466896A1 (en) * | 2001-09-06 | 2003-03-20 | Adnagen Ag | Method and diagnosis kit for selecting and or qualitative and/or quantitative detection of cells |
| EP1409745B1 (en) * | 2001-09-06 | 2007-04-11 | Adnagen AG | Method and kit for the diagnosis or treatment control of intestinal carcinoma |
| WO2003023571A2 (en) * | 2001-09-12 | 2003-03-20 | Burstein Technologies, Inc. | Methods for differential cell counts including related apparatus and software for performing same |
| US8980568B2 (en) | 2001-10-11 | 2015-03-17 | Aviva Biosciences Corporation | Methods and compositions for detecting non-hematopoietic cells from a blood sample |
| US8986944B2 (en) | 2001-10-11 | 2015-03-24 | Aviva Biosciences Corporation | Methods and compositions for separating rare cells from fluid samples |
| AU2003216175A1 (en) * | 2002-02-04 | 2003-09-02 | Colorado School Of Mines | Laminar flow-based separations of colloidal and cellular particles |
| KR20040105717A (en) * | 2002-02-14 | 2004-12-16 | 이뮤니베스트 코포레이션 | Methods and algorithms for cell enumeration in a low-cost cytometer |
| US7764821B2 (en) * | 2002-02-14 | 2010-07-27 | Veridex, Llc | Methods and algorithms for cell enumeration in a low-cost cytometer |
| WO2004011128A1 (en) * | 2002-07-26 | 2004-02-05 | Aclara Biosciences, Inc. | Methods and compositions for screening cell binding molecules |
| ES2375724T3 (en) * | 2002-09-27 | 2012-03-05 | The General Hospital Corporation | MICROFLUDE DEVICE FOR SEPERATION OF CELLS AND ITS USES. |
| WO2004077021A2 (en) * | 2003-02-27 | 2004-09-10 | Lesko Stephen A | Standardized evaluation of therapeutic efficacy based on cellular biomarkers |
| MXPA05012080A (en) * | 2003-05-08 | 2006-02-22 | Xcyte Therapies Inc | Generation and isolation of antigen-specific t cells. |
| US20070160503A1 (en) * | 2003-06-13 | 2007-07-12 | Palaniappan Sethu | Microfluidic systems for size based removal of red blood cells and platelets from blood |
| EP1494028A1 (en) * | 2003-07-03 | 2005-01-05 | Labsoft Diagnostics AG | Immunomagnetic separation of specific target cells |
| US20050020899A1 (en) * | 2003-07-25 | 2005-01-27 | Rubicor Medical, Inc. | Post-biopsy cavity treatmetn implants and methods |
| CA2539716A1 (en) * | 2003-09-22 | 2005-04-07 | Xcyte Therapies, Inc. | Compositions and methods to accelerate hematologic recovery |
| AU2005218622A1 (en) * | 2004-03-03 | 2005-09-15 | Living Microsystems | Magnetic device for isolation of cells and biomolecules in a microfluidic environment |
| US8189899B2 (en) * | 2004-07-30 | 2012-05-29 | Veridex, Llc | Methods and algorithms for cell enumeration in a low-cost cytometer |
| WO2006020936A2 (en) * | 2004-08-12 | 2006-02-23 | Immunivest Corporation | A method for assessing disease states by profile analysis of isolated circulating endothelial cells |
| US20060171846A1 (en) * | 2005-01-10 | 2006-08-03 | Marr David W M | Microfluidic systems incorporating integrated optical waveguides |
| US20070196820A1 (en) | 2005-04-05 | 2007-08-23 | Ravi Kapur | Devices and methods for enrichment and alteration of cells and other particles |
| US20070026417A1 (en) * | 2005-07-29 | 2007-02-01 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
| US20070026415A1 (en) * | 2005-07-29 | 2007-02-01 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
| US20070026413A1 (en) * | 2005-07-29 | 2007-02-01 | Mehmet Toner | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
| US20070026414A1 (en) * | 2005-07-29 | 2007-02-01 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
| CA2609965A1 (en) * | 2005-05-27 | 2006-11-30 | Board Of Regents, The University Of Texas System | Optical coherence tomographic detection of cells and compositions |
| US8921102B2 (en) | 2005-07-29 | 2014-12-30 | Gpb Scientific, Llc | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
| US20070059680A1 (en) * | 2005-09-15 | 2007-03-15 | Ravi Kapur | System for cell enrichment |
| US20070026416A1 (en) * | 2005-07-29 | 2007-02-01 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
| US20090181421A1 (en) * | 2005-07-29 | 2009-07-16 | Ravi Kapur | Diagnosis of fetal abnormalities using nucleated red blood cells |
| US7901950B2 (en) * | 2005-08-12 | 2011-03-08 | Veridex, Llc | Method for assessing disease states by profile analysis of isolated circulating endothelial cells |
| US20070059774A1 (en) * | 2005-09-15 | 2007-03-15 | Michael Grisham | Kits for Prenatal Testing |
| US20070059683A1 (en) * | 2005-09-15 | 2007-03-15 | Tom Barber | Veterinary diagnostic system |
| US20070059718A1 (en) * | 2005-09-15 | 2007-03-15 | Mehmet Toner | Systems and methods for enrichment of analytes |
| US20070059719A1 (en) * | 2005-09-15 | 2007-03-15 | Michael Grisham | Business methods for prenatal Diagnosis |
| US20070059781A1 (en) * | 2005-09-15 | 2007-03-15 | Ravi Kapur | System for size based separation and analysis |
| US7807459B2 (en) * | 2005-09-27 | 2010-10-05 | Reneuron, Inc. | EphA4-positive human adult pancreatic endocrine progenitor cells |
| US8119976B2 (en) * | 2007-07-03 | 2012-02-21 | Colorado School Of Mines | Optical-based cell deformability |
| US9885644B2 (en) | 2006-01-10 | 2018-02-06 | Colorado School Of Mines | Dynamic viscoelasticity as a rapid single-cell biomarker |
| US9487812B2 (en) | 2012-02-17 | 2016-11-08 | Colorado School Of Mines | Optical alignment deformation spectroscopy |
| US9878326B2 (en) * | 2007-09-26 | 2018-01-30 | Colorado School Of Mines | Fiber-focused diode-bar optical trapping for microfluidic manipulation |
| US20080076727A1 (en) * | 2006-03-29 | 2008-03-27 | John Wayne Cancer Institute | Utility of high molecular weight melanoma associated antigen in diagnosis and treatment of cancer |
| US20080050739A1 (en) * | 2006-06-14 | 2008-02-28 | Roland Stoughton | Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats |
| WO2008111990A1 (en) * | 2006-06-14 | 2008-09-18 | Cellpoint Diagnostics, Inc. | Rare cell analysis using sample splitting and dna tags |
| US20080070792A1 (en) | 2006-06-14 | 2008-03-20 | Roland Stoughton | Use of highly parallel snp genotyping for fetal diagnosis |
| EP2589668A1 (en) | 2006-06-14 | 2013-05-08 | Verinata Health, Inc | Rare cell analysis using sample splitting and DNA tags |
| US8137912B2 (en) | 2006-06-14 | 2012-03-20 | The General Hospital Corporation | Methods for the diagnosis of fetal abnormalities |
| US20080007838A1 (en) * | 2006-07-07 | 2008-01-10 | Omnitech Partners, Inc. | Field-of-view indicator, and optical system and associated method employing the same |
| CN101583722A (en) * | 2006-07-14 | 2009-11-18 | 阿维瓦生物科学股份有限公司 | Methods and compositions for detecting rare cells from a biological sample |
| US10722250B2 (en) | 2007-09-04 | 2020-07-28 | Colorado School Of Mines | Magnetic-field driven colloidal microbots, methods for forming and using the same |
| US20090062828A1 (en) * | 2007-09-04 | 2009-03-05 | Colorado School Of Mines | Magnetic field-based colloidal atherectomy |
| SI2229591T1 (en) | 2007-12-05 | 2012-12-31 | Zyomyx, Inc. | Cell assay kit and method |
| US8071395B2 (en) * | 2007-12-12 | 2011-12-06 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and apparatus for magnetic separation of cells |
| KR101384142B1 (en) * | 2007-12-28 | 2014-04-14 | 삼성디스플레이 주식회사 | Display substrate, method for manufacturing the display substrate and display apparatus having the display substrate |
| US7927561B2 (en) * | 2008-01-10 | 2011-04-19 | Becton, Dickinson And Company | Rapid particle detection assay |
| SI2334812T1 (en) | 2008-09-20 | 2017-05-31 | The Board of Trustees of the Leland Stanford Junior University Office of the General Counsel Building 170 | Noninvasive diagnosis of fetal aneuploidy by sequencing |
| US20120100538A1 (en) * | 2009-03-24 | 2012-04-26 | Biocept, Inc. | Devices and methods of cell capture and analysis |
| WO2010111388A2 (en) * | 2009-03-24 | 2010-09-30 | Biocept, Inc. | Devices and methods of cell capture and analysis |
| US8187979B2 (en) * | 2009-12-23 | 2012-05-29 | Varian Semiconductor Equipment Associates, Inc. | Workpiece patterning with plasma sheath modulation |
| US10539487B2 (en) | 2010-03-04 | 2020-01-21 | Ventana Medical Systems, Inc. | Systems and methods for monitoring tissue sample processing |
| US10126216B2 (en) | 2011-02-17 | 2018-11-13 | Ventana Medical Systems, Inc. | Method for tissue sample fixation |
| CA2788664C (en) | 2010-03-04 | 2023-04-11 | Ventana Medical Systems, Inc. | Processing system for processing specimens using acoustic energy |
| US20120020938A1 (en) * | 2010-07-26 | 2012-01-26 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | MHC- less cells |
| CN103180733B (en) * | 2010-08-31 | 2015-11-25 | 协和梅迪克斯株式会社 | The assay method of FGF-23 and mensuration reagent |
| KR20120026959A (en) | 2010-09-10 | 2012-03-20 | 인제대학교 산학협력단 | Microparticle separator based on magnetophoresis and microparticle separating method using the same |
| KR101213971B1 (en) | 2010-09-14 | 2012-12-20 | 서울대학교산학협력단 | method and device for remote moving of organelle, and magnetic particle complex for the same |
| US20130217049A1 (en) * | 2012-02-21 | 2013-08-22 | Laboratory Corporation Of America Holdings | Methods and Systems for Signal Amplification of Bioassays |
Family Cites Families (56)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4219411A (en) * | 1978-09-18 | 1980-08-26 | California Institute Of Technology | Cell sorting apparatus |
| US4230685A (en) * | 1979-02-28 | 1980-10-28 | Northwestern University | Method of magnetic separation of cells and the like, and microspheres for use therein |
| US4510244A (en) | 1980-04-17 | 1985-04-09 | The Board Of Trustees Of The Leland Stanford Jr. University | Cell labeling with antigen-coupled microspheres |
| US4497900A (en) * | 1982-04-12 | 1985-02-05 | Abbott Laboratories | Immunoassay for Neisseria gonorrhoeae antigens |
| US4511662A (en) | 1982-06-18 | 1985-04-16 | Bio-Rad Laboratories, Inc. | Simultaneous assay for T and B lymphocyte populations and subpopulations |
| AU563827B2 (en) | 1982-07-01 | 1987-07-23 | Millipore Corp. | Filtration apparatus |
| US4659678A (en) | 1982-09-29 | 1987-04-21 | Serono Diagnostics Limited | Immunoassay of antigens |
| US4925922A (en) | 1983-02-22 | 1990-05-15 | Xoma Corporation | Potentiation of cytotoxic conjugates |
| US4664911A (en) | 1983-06-21 | 1987-05-12 | Board Of Regents, University Of Texas System | Immunotoxin conjugates employing toxin B chain moieties |
| US4704255A (en) | 1983-07-15 | 1987-11-03 | Pandex Laboratories, Inc. | Assay cartridge |
| US4920061A (en) * | 1984-03-02 | 1990-04-24 | The University Of Texas System | Biological magnetic colloids |
| US4752569A (en) * | 1984-06-21 | 1988-06-21 | The Regents Of The University Of California | Sialylated Lewisx epitope, antibodies and diagnosis |
| US5019497A (en) * | 1984-11-09 | 1991-05-28 | Lennart Olsson | Human squamous lung carcinoma cell specific antigens and antibodies |
| US4710472A (en) * | 1985-09-25 | 1987-12-01 | The United States Of America As Represented By The Secretary Of The Navy | Magnetic separation device |
| US5076950A (en) | 1985-12-20 | 1991-12-31 | Syntex (U.S.A.) Inc. | Magnetic composition for particle separation |
| EP0241042A3 (en) | 1986-04-11 | 1988-05-04 | Hitachi, Ltd. | A method for cell analysis |
| EP0256471A3 (en) | 1986-08-15 | 1989-10-25 | Xoma Corporation | Cytotoxic conjugates for cancer therapy |
| US4895706A (en) | 1986-10-28 | 1990-01-23 | Costar Corporation | Multi-well filter strip and composite assemblies |
| US4857452A (en) * | 1986-12-04 | 1989-08-15 | E. I. Du Pont De Nemours And Company | Assay for carcinoma of breast, colon and ovary |
| JPH01502195A (en) | 1987-01-27 | 1989-08-03 | ゾーマ・コーポレーション | Method for enhancing cytotoxic conjugates |
| US4847199A (en) | 1987-02-27 | 1989-07-11 | Eastman Kodak Company | Agglutination immunoassay and kit for determination of a multivalent immune species using a buffered salt wash solution |
| JPH07104349B2 (en) | 1987-04-11 | 1995-11-13 | 株式会社日立製作所 | Cytometry |
| US5194300A (en) | 1987-07-15 | 1993-03-16 | Cheung Sau W | Methods of making fluorescent microspheres |
| US5322678A (en) | 1988-02-17 | 1994-06-21 | Neorx Corporation | Alteration of pharmacokinetics of proteins by charge modification |
| US5385822A (en) | 1988-05-02 | 1995-01-31 | Zynaxis, Inc. | Methods for detection and quantification of cell subsets within subpopulations of a mixed cell population |
| US5256532A (en) | 1988-05-02 | 1993-10-26 | Zynaxis Technologies, Inc. | Methods, reagents and test kits for determination of subpopulations of biological entities |
| FR2638849B1 (en) | 1988-11-04 | 1994-03-18 | Chemunex Sa | METHOD FOR AMPLIFYING A FLUORESCENT SIGNAL FOR THE SPECIFIC SEARCH OF THE POSSIBLE PRESENCE OF PARTICLES AND APPLICATION TO THE DETECTION AND NUMBERING OF SAID PARTICLES |
| FR2638848B1 (en) | 1988-11-04 | 1993-01-22 | Chemunex Sa | METHOD OF DETECTION AND / OR DETERMINATION IN A LIQUID OR SEMI-LIQUID MEDIUM OF AT LEAST ONE ORGANIC, BIOLOGICAL OR MEDICINAL SUBSTANCE, BY AN AGGLUTINATION METHOD |
| DE68919715T2 (en) * | 1988-12-28 | 1995-04-06 | Stefan Miltenyi | METHOD AND MATERIALS FOR HIGHLY GRADUATED MAGNETIC SPLITTING OF BIOLOGICAL MATERIALS. |
| GB8905001D0 (en) | 1989-03-04 | 1989-04-19 | Univ Leicester | Screening for natural products of microbial metabolism |
| WO1990010692A1 (en) * | 1989-03-15 | 1990-09-20 | University Of Florida | Monoclonal antibody for use in detection and treatment of childhood leukemia |
| US5081030A (en) * | 1989-04-25 | 1992-01-14 | The Johns Hopkins University | Release of cells from affinity matrices |
| DE3919923A1 (en) * | 1989-06-19 | 1990-12-20 | Behringwerke Ag | MAGNETIC PROTEIN CONJUGATES, METHOD FOR THE PRODUCTION THEREOF AND THEIR USE |
| DE3919873A1 (en) | 1989-06-19 | 1990-12-20 | Behringwerke Ag | MAGNETIC PROTEIN CONJUGATES, METHOD FOR THE PRODUCTION THEREOF AND THEIR USE |
| GB8916859D0 (en) * | 1989-07-24 | 1989-09-06 | Dynal As | Hapten linking |
| DE69033631T2 (en) | 1989-12-14 | 2001-05-17 | Sloan Kettering Inst Cancer | THERAPEUTIC USE OF THE HYPERVARIABLE REGION OF THE MONOCLONAL ANTIBODY M195 AND CONSTRUCTS THEREOF |
| GB8929297D0 (en) * | 1989-12-29 | 1990-02-28 | Dynal As | Method of separation |
| GB9007966D0 (en) | 1990-04-09 | 1990-06-06 | Dynal As | Antigen/anti-antigen cleavage |
| US5200084A (en) * | 1990-09-26 | 1993-04-06 | Immunicon Corporation | Apparatus and methods for magnetic separation |
| US5340719A (en) | 1990-11-23 | 1994-08-23 | Corporation Coulter | Method and apparatus for optically screening microscopic cells |
| JPH05107249A (en) | 1991-02-04 | 1993-04-27 | Toyobo Co Ltd | High-sensitivity detection method of ligand/receptor reaction |
| US5491068A (en) | 1991-02-14 | 1996-02-13 | Vicam, L.P. | Assay method for detecting the presence of bacteria |
| AU2115092A (en) | 1991-10-08 | 1993-04-22 | Eastman Kodak Company | Method, test device and kit for assay of specific binding ligand using controlled flow through filtration membrane |
| US5290707A (en) | 1991-11-25 | 1994-03-01 | The United States Of America As Represented By The Secretary Of The Army | Method for detection of microorganisms |
| US5256542A (en) | 1992-03-09 | 1993-10-26 | Tanox Biosystems, Inc. | Selecting low frequency antigen-specific single B lymphocytes with correction for background noise |
| WO1993019199A1 (en) | 1992-03-20 | 1993-09-30 | Celsis Limited | Method and apparatus for the analysis of biological material |
| US5422277A (en) | 1992-03-27 | 1995-06-06 | Ortho Diagnostic Systems Inc. | Cell fixative composition and method of staining cells without destroying the cell surface |
| CA2141428A1 (en) | 1992-07-28 | 1994-02-03 | Steven Kessler | Methods for positive immunoselection of stem cells |
| DK0660936T3 (en) | 1992-09-14 | 1999-05-25 | Stanford Res Inst Int | Up-converting reporter molecule for biological and other assays using laser excitation technique |
| AU2593192A (en) | 1992-09-14 | 1994-04-12 | Oystein Fodstad | Detection of specific target cells in specialized or mixed cell population and solutions containing mixed cell populations |
| FR2700010B1 (en) | 1992-12-24 | 1995-03-17 | Rocher Yves Biolog Vegetale | Method and device for testing the reactivity of cells with regard to products. |
| US5374531A (en) | 1993-03-22 | 1994-12-20 | Zynaxis, Inc. | Immunoassay for determination of cells |
| US5514340A (en) | 1994-01-24 | 1996-05-07 | Magnetix Biotechnology, Inc. | Device for separating magnetically labelled cells |
| NO180658C (en) | 1994-03-10 | 1997-05-21 | Oeystein Fodstad | Method and Device for Detecting Specific Target Cells in Specialized or Mixed Cell Populations and Solutions Containing Mixed Cell Populations |
| US6017719A (en) | 1994-06-14 | 2000-01-25 | Nexell Therapeutics, Inc. | Positive and positive/negative cell selection mediated by peptide release |
| AUPN214095A0 (en) | 1995-04-03 | 1995-04-27 | Australian Water Technologies Pty Ltd | Method for detecting microorganisms using flow cytometry |
-
1992
- 1992-09-14 AU AU25931/92A patent/AU2593192A/en not_active Abandoned
- 1992-09-14 WO PCT/NO1992/000151 patent/WO1994007138A1/en active Application Filing
-
1993
- 1993-09-10 PL PL93308109A patent/PL177136B1/en unknown
- 1993-09-10 EP EP93921134A patent/EP0660930B1/en not_active Revoked
- 1993-09-10 US US08/403,844 patent/US6893881B1/en not_active Expired - Lifetime
- 1993-09-10 ES ES93921134T patent/ES2141170T3/en not_active Expired - Lifetime
- 1993-09-10 CA CA2144328A patent/CA2144328C/en not_active Expired - Lifetime
- 1993-09-10 JP JP50799194A patent/JP3417563B2/en not_active Expired - Lifetime
- 1993-09-10 DK DK93921134T patent/DK0660930T3/en active
- 1993-09-10 DE DE69326956T patent/DE69326956T2/en not_active Revoked
- 1993-09-10 CZ CZ95659A patent/CZ65995A3/en unknown
- 1993-09-10 PT PT93921134T patent/PT660930E/en unknown
- 1993-09-10 AU AU48363/93A patent/AU686569B2/en not_active Ceased
- 1993-09-10 SK SK329-95A patent/SK281566B6/en unknown
- 1993-09-10 HU HU9500723A patent/HU221234B1/en not_active IP Right Cessation
- 1993-09-10 WO PCT/NO1993/000136 patent/WO1994007139A1/en not_active Application Discontinuation
- 1993-09-10 AT AT93921134T patent/ATE186402T1/en not_active IP Right Cessation
-
1995
- 1995-03-13 FI FI951161A patent/FI951161A/en unknown
-
1997
- 1997-06-24 US US08/881,393 patent/US6184043B1/en not_active Ceased
-
2000
- 2000-02-02 GR GR20000400243T patent/GR3032547T3/en not_active IP Right Cessation
-
2003
- 2003-02-05 US US10/359,677 patent/USRE43979E1/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CZ65995A3 (en) | 1995-11-15 |
| CA2144328C (en) | 2010-11-30 |
| DE69326956D1 (en) | 1999-12-09 |
| FI951161A (en) | 1995-05-09 |
| AU686569B2 (en) | 1998-02-12 |
| DE69326956T2 (en) | 2000-06-15 |
| GR3032547T3 (en) | 2000-05-31 |
| HU221234B1 (en) | 2002-08-28 |
| US6184043B1 (en) | 2001-02-06 |
| EP0660930A1 (en) | 1995-07-05 |
| USRE43979E1 (en) | 2013-02-05 |
| ES2141170T3 (en) | 2000-03-16 |
| EP0660930B1 (en) | 1999-11-03 |
| US6893881B1 (en) | 2005-05-17 |
| HUT73741A (en) | 1996-09-30 |
| PL177136B1 (en) | 1999-09-30 |
| SK32995A3 (en) | 1995-10-11 |
| JP3417563B2 (en) | 2003-06-16 |
| WO1994007138A1 (en) | 1994-03-31 |
| JPH08501390A (en) | 1996-02-13 |
| WO1994007139A1 (en) | 1994-03-31 |
| ATE186402T1 (en) | 1999-11-15 |
| HU9500723D0 (en) | 1995-04-28 |
| FI951161A0 (en) | 1995-03-13 |
| SK281566B6 (en) | 2001-05-10 |
| AU2593192A (en) | 1994-04-12 |
| AU4836393A (en) | 1994-04-12 |
| DK0660930T3 (en) | 2000-05-08 |
| PT660930E (en) | 2000-04-28 |
| PL308109A1 (en) | 1995-07-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2144328A1 (en) | Improved method for detection of specific target cells in specialized or mixed cell population and solutions containing mixed cell populations | |
| JP4071824B2 (en) | Method for isolating and detecting specific target cells in a cell suspension of a mixed cell population | |
| FI86077C (en) | MONOCLONAL ANTIKROPPAR OCH METOD ATT PRODUCERA DESSA. | |
| CN106996976B (en) | CTC protein parting kits based on microflow control technique | |
| EP2291653A1 (en) | Assay | |
| EP0401370A1 (en) | Enzyme immunoassay according to sandwich method of human iv-type collagen | |
| JPH04502363A (en) | Detection of basement membrane components for diagnosis of cancer and other diseases | |
| JP2013513812A (en) | Assay | |
| CN117607463B (en) | EGFR (epidermal growth factor receptor) immune chromogenic detection reagent for circulating tumor cells | |
| CN117607441B (en) | Circulating tumor cell TROP-2 immune chromogenic detection reagent | |
| CN115466731B (en) | Hybridoma cell strain screening method | |
| JPS59173760A (en) | Method and reagent for immunochemical measurement | |
| EP0891549A2 (en) | Foetal cell analysis | |
| US20150355170A1 (en) | Compositions, systems and methods that detect and/or remove cross-reactive antibodies from a biological sample | |
| Milios et al. | Avidin-biotin-peroxidase complex reagents for routine histological application: a comparison of four commercial kits | |
| KR20030094547A (en) | A monoclonal antibody screening method for selection of hybridoma cell line producing monoclonal antibodies and kit for selection of hybridoma cell | |
| Li et al. | A Panel of Rabbit Monoclonal Antibody Marker for Prostate Cancer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |