CA2146235A1 - Process for growing thraustochytriales - Google Patents

Process for growing thraustochytriales

Info

Publication number
CA2146235A1
CA2146235A1 CA002146235A CA2146235A CA2146235A1 CA 2146235 A1 CA2146235 A1 CA 2146235A1 CA 002146235 A CA002146235 A CA 002146235A CA 2146235 A CA2146235 A CA 2146235A CA 2146235 A1 CA2146235 A1 CA 2146235A1
Authority
CA
Canada
Prior art keywords
schizochytrium
thraustochytrium
mixtures
microns
culture medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002146235A
Other languages
French (fr)
Other versions
CA2146235C (en
Inventor
William R. Barclay
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
William R. Barclay
Phycotech, Inc.
Omegatech, Inc.
Martek Biosciences Boulder Corporation
Martek Biosciences Corporation
Dsm Ip Assets B.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25506004&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2146235(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by William R. Barclay, Phycotech, Inc., Omegatech, Inc., Martek Biosciences Boulder Corporation, Martek Biosciences Corporation, Dsm Ip Assets B.V. filed Critical William R. Barclay
Publication of CA2146235A1 publication Critical patent/CA2146235A1/en
Application granted granted Critical
Publication of CA2146235C publication Critical patent/CA2146235C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/946—Microorganisms using algae

Abstract

Disclosed is a process for growing the microflora Thraustochytrium, Schizochytrium and mixtures thereof, which includes the growing of the microflora in fermentation medium containing non-chloride containing sodium salts, in particular sodium sulfate.
In a preferred embodiment of the present invention, the process produces microflora having a cell aggregate size useful for the production of food products for use in aquaculture. Further disclosed is a food product which includes Thraustochytrium, Schizochytrium, and mixtures thereof, and a component selected from flaxseed, rapeseed, soybean and avocado meal. Such a food product includes a balance of long chain and short chain omega-3 highly unsaturated fatty acids.

Claims (37)

What is claimed is:
1. A process for growing Thraustochytrium, Schizochytrium, and mixtures thereof, comprising growing said Thraustochytrium, Schizochytrium, and mixtures thereof, in a culture medium containing less than about 3 grams of chloride per liter of said culture medium, sources of carbon, nitrogen, micronutrients, and a non-chloride sodium salt at a temperature from about 5°C to about 48°C and at a pH from about pH 5.0 to about pH 11Ø
2. The process of claim 1, wherein said sodium salt is selected from the group consisting of sodium sulfate, soda ash, sodium oxide, sodium carbonate, sodium bicarbonate, sodium sulfate and mixtures thereof.
3. The process of claim 2, wherein the concentration of said sodium sulfate, expressed as grams of sodium per liter of said culture medium, is greater than about 1Ø
4. The process of claim 2, wherein the concentration of said sodium sulfate, expressed as grams of sodium per liter of said culture medium, is between about 1.0 and about 50Ø
5. The process of claim 2, wherein the concentration of said sodium sulfate, expressed as grams of sodium per liter of said culture medium, is between about 2.0 and about 25Ø
6. The process of claim 1, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof, have all of the identifying characteristics of a biologically pure microorganism selected from the group consisting of ATCC

Nos. 20888 and 20889, and mutant strains derived therefrom, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof, have an omega-3 highly unsaturated fatty acid content of at least about 0.5% dry weight.
7. The process of claim 1, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof, have a sterol content of at least about 0.1% ash free dry weight.
8. The process of Claim 7, wherein Thraustochytrium, Schizochytrium, and mixtures thereof, have a cholesterol content of at least about 15% of the total sterol content.
9. The process of Claim 1, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof, have a cell aggregate size less than about 150 microns in diameter.
10. The process of Claim 1, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof, have a cell aggregate size less than about 100 microns in diameter.
11. The process of Claim 1, wherein said
Thraustochytrium, Schizochytrium, and mixtures thereof, have a cell aggregate size less than about 50 microns in diameter.
13. The process of Claim 1, wherein said culture medium has a chloride concentration of less than about 250 milligrams of chloride per liter of culture medium.
14. A microfloral biomass comprising Thraustochytrium, Schizochytrium, and mixtures thereof, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof have a cell aggregate size less than about 150 microns.
15. The microfloral biomass of Claim 14, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof have been grown in a culture medium containing sodium sulfate.
16. The microfloral biomass of Claim 14, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof, have all of the identifying characteristics of an organism selected from the group consisting of ATCC Nos. 20888 and 20889, and mutants thereof, wherein said mutants have an omega-3 HUFA content of at least about 0.5% dwt.
17. The microfloral biomass of Claim 14, wherein of said Thraustochytrium, Schizochytrium, and mixtures thereof have a sterol content of at least about 0.1% afdw.
18. The microfloral biomass of Claim 17, wherein Thraustochytrium and Schizochytrium have a cholesterol content of at least about 15% of the total sterol content.
19. The microfloral biomass of Claim 14, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof have a cell aggregate size less than about 100 microns.
20. The microfloral biomass of Claim 14, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof have a cell aggregate size less than about 50 microns.
21. A method to produce shrimp, comprising feeding microflora selected from the group consisting of Thraustochytrium, Schizochytrium, and mixtures thereof to larval shrimp, said microflora having a cell aggregate size less than about 150 microns.
22. The method of Claim 21, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof have been grown in a culture medium containing sodium sulfate.
23. The method of Claim 21, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof, have all of the identifying characteristics of an organism selected from the group consisting of ATCC Nos. 20888 and 20889, and mutants thereof, wherein said mutants have an omega-3 HUFA content of at least about 0.5% dry weight.
24. The method of Claim 21, wherein said Thraustochytrium and Schizochytrium have a sterol content of at least about 0.1% afdw.
25. The method of Claim 24, wherein said Thraustochytrium and Schizochytrium have a cholesterol content of at least about 15% of the total sterol content.
26. The method of Claim 21, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof have a cell aggregate size less than about 100 microns.
27. The method of Claim 21, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof have a cell aggregate size less than about 50 microns.
28. A food product comprising:
a) microflora selected from the group consisting of Thraustochytrium, Schizochytrium, and mixtures thereof; and b) a nutrient selected from the group consisting of flaxseed, rapeseed, soybean, avocado meal, and mixtures thereof.
29. The food product of Claim 28, wherein said composition comprises between about 5% and about 95% by weight of said microflora.
30. The food product of Claim 28, wherein said composition comprises between about 5% and about 95% by weight of flaxseed.
31. The food product of Claim 28, wherein said composition comprises between about 5% and about 95% by weight of rapeseed.
32. The food product of Claim 28, wherein said composition comprises between about 5% and about 95% by weight of soybean.
33. The food product of Claim 28, wherein said composition comprises between about 5% and about 95% by weight of avocado meal.
34. The food product of Claim 28, wherein said food product is an extruded product.
35. A method of aquaculture, comprising feeding microflora selected from the group consisting of Thraustochytrium, Schizochytrium, and mixtures thereof to organisms selected from the group consisting of larval shrimp, brine shrimp, rotifers and mollusks, said microflora having a cell aggregate size less than about 150 microns.
36. The method of Claim 35, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof have been grown in a culture medium containing sodium sulfate.
37. The method of Claim 35, wherein said Thraustochytrium, Schizochytrium, and mixtures thereof, have all of the identifying characteristics of an organism selected from the group consisting of ATCC Nos. 20888 and 20889, and mutants thereof, wherein said mutants have an omega-3 HUFA content of at least about 0.5% dry weight.
CA2146235A 1992-10-16 1993-10-12 Process for growing thraustochytriales Expired - Lifetime CA2146235C (en)

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US07/962,522 1992-10-16
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