CA2147593A1 - Dual purpose tissue fixative - Google Patents

Dual purpose tissue fixative

Info

Publication number
CA2147593A1
CA2147593A1 CA002147593A CA2147593A CA2147593A1 CA 2147593 A1 CA2147593 A1 CA 2147593A1 CA 002147593 A CA002147593 A CA 002147593A CA 2147593 A CA2147593 A CA 2147593A CA 2147593 A1 CA2147593 A1 CA 2147593A1
Authority
CA
Canada
Prior art keywords
fixative
tissue
alcohol
tissue fixative
dual purpose
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002147593A
Other languages
French (fr)
Other versions
CA2147593C (en
Inventor
Hyman C. Birnboim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cancer Care Ontario
Original Assignee
Hyman C. Birnboim
The Ontario Cancer Treatment And Research Foundation
Cancer Care Ontario
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hyman C. Birnboim, The Ontario Cancer Treatment And Research Foundation, Cancer Care Ontario filed Critical Hyman C. Birnboim
Publication of CA2147593A1 publication Critical patent/CA2147593A1/en
Application granted granted Critical
Publication of CA2147593C publication Critical patent/CA2147593C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N2001/305—Fixative compositions
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/108331—Preservative, buffer, anticoagulant or diluent

Abstract

A dual purpose tissue fixative is described. The fixative comprises a cross-linking aldehyde, alcohol, a chelating agent and a non-amine buffer. More particularly, the tissue fixative comprises 0.1-2 % w/v formaldehyde, 45-90% w/w alcohol, 1-10 mM of a chelating agent and 1-50 mM of a non-nitrogen buffer. The tissue fixative permits the recovery of high molecular weight DNA and RNA from the tissue sample for molecular genetic analysis. The tissue fixative also preserves the morphology and immunogenicity of the tissue allowing for pathology analysis. The tissue fixative is compared to 95% alcohol and a standard fixative such as 10% BNF.
CA002147593A 1994-04-22 1995-04-21 Dual purpose tissue fixative Expired - Fee Related CA2147593C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23124794A 1994-04-22 1994-04-22
US08/231,247 1994-04-22

Publications (2)

Publication Number Publication Date
CA2147593A1 true CA2147593A1 (en) 1995-10-23
CA2147593C CA2147593C (en) 2008-07-29

Family

ID=22868391

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002147593A Expired - Fee Related CA2147593C (en) 1994-04-22 1995-04-21 Dual purpose tissue fixative

Country Status (2)

Country Link
US (1) US5976829A (en)
CA (1) CA2147593C (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999009390A1 (en) * 1997-08-20 1999-02-25 The University Of Miami A high quality, continuous throughput, tissue fixation-dehydration-fat removal-impregnation method
WO2000000813A1 (en) * 1998-06-30 2000-01-06 Lamina, Inc. Cytological and histological fixative composition and methods of use
WO2001098542A2 (en) * 2000-06-21 2001-12-27 Digene Corporation Universal collection medium
US20050106155A1 (en) * 2002-03-05 2005-05-19 Tadao Ohno Solidified tissue immuoadjuvant
US7138226B2 (en) * 2002-05-10 2006-11-21 The University Of Miami Preservation of RNA and morphology in cells and tissues
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
JP4527122B2 (en) * 2003-10-24 2010-08-18 ザ ユニバーシティー オブ マイアミ Simplify organizational processing
WO2007016935A1 (en) 2005-07-29 2007-02-15 Histogenex Nv Methods, reagents and instrumentation for preparing impregnated tissue samples suitable for histopathological and molecular studies
AU2009307661B2 (en) * 2008-10-21 2014-11-20 Thomas M. Donndelinger Methods and compositions for preventing artifacts in tissue samples
WO2011157678A1 (en) 2010-06-14 2011-12-22 Qiagen Gmbh Method for determination of target cells or tissue for extraction of biomolecules from fixed biological samples
KR101133997B1 (en) 2011-04-06 2012-04-05 (주)셀트라존 A composite for the use of cytological analysis
EP3928715A1 (en) 2011-06-19 2021-12-29 DNA Genotek, Inc. Devices, solutions and methods for sample collection
US10107727B2 (en) 2015-12-30 2018-10-23 Sakura Finetek U.S.A., Inc. Tissue processing reagent
US9835527B2 (en) 2015-12-30 2017-12-05 Sakura Finetek U.S.A., Inc. Tissue processing reagent
US10174362B2 (en) 2017-01-16 2019-01-08 Spectrum Solutions L.L.C. Nucleic acid preservation solution and methods of manufacture and use
AU2019384801A1 (en) 2018-11-20 2021-06-10 Spectrum Solutions, Llc Sample collection system including sealing cap and valve
US11701094B2 (en) 2019-06-20 2023-07-18 Spectrum Solutions L.L.C. Sample collection system including valve and plug assemblies
EP4145105A1 (en) 2021-09-06 2023-03-08 Anacyte Laboratories GmbH Cell asservation solution

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4888278A (en) * 1985-10-22 1989-12-19 University Of Massachusetts Medical Center In-situ hybridization to detect nucleic acid sequences in morphologically intact cells
US5389549A (en) * 1987-05-29 1995-02-14 Toa Medical Electronics Co., Ltd. Method for classifying leukocytes and a reagent used therefor
US4857300A (en) * 1987-07-27 1989-08-15 Cytocorp, Inc. Cytological and histological fixative formulation and methods for using same
US4826691A (en) * 1987-08-03 1989-05-02 Berkley, Inc. Carrier for fish attractant
JPH068818B2 (en) * 1987-11-27 1994-02-02 サクラ精機株式会社 Fixative for biopsy
US5196182A (en) * 1991-05-08 1993-03-23 Streck Laboratories, Inc. Tissue fixative
US5357977A (en) * 1993-04-23 1994-10-25 St. Mary's Hospital And Medical Center, Inc. Cytological sampling method and device
US5405606A (en) * 1993-12-03 1995-04-11 Efh, Inc. Embalming composition and method

Also Published As

Publication number Publication date
CA2147593C (en) 2008-07-29
US5976829A (en) 1999-11-02

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Effective date: 20140422