CA2147593A1 - Dual purpose tissue fixative - Google Patents
Dual purpose tissue fixativeInfo
- Publication number
- CA2147593A1 CA2147593A1 CA002147593A CA2147593A CA2147593A1 CA 2147593 A1 CA2147593 A1 CA 2147593A1 CA 002147593 A CA002147593 A CA 002147593A CA 2147593 A CA2147593 A CA 2147593A CA 2147593 A1 CA2147593 A1 CA 2147593A1
- Authority
- CA
- Canada
- Prior art keywords
- fixative
- tissue
- alcohol
- tissue fixative
- dual purpose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000834 fixative Substances 0.000 title abstract 8
- 230000009977 dual effect Effects 0.000 title abstract 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract 2
- 239000002738 chelating agent Substances 0.000 abstract 2
- 150000001412 amines Chemical class 0.000 abstract 1
- 238000004132 cross linking Methods 0.000 abstract 1
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 abstract 1
- 238000012252 genetic analysis Methods 0.000 abstract 1
- 230000005847 immunogenicity Effects 0.000 abstract 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 abstract 1
- 229910052757 nitrogen Inorganic materials 0.000 abstract 1
- 230000007170 pathology Effects 0.000 abstract 1
- 238000011084 recovery Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/305—Fixative compositions
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/108331—Preservative, buffer, anticoagulant or diluent
Abstract
A dual purpose tissue fixative is described. The fixative comprises a cross-linking aldehyde, alcohol, a chelating agent and a non-amine buffer. More particularly, the tissue fixative comprises 0.1-2 % w/v formaldehyde, 45-90% w/w alcohol, 1-10 mM of a chelating agent and 1-50 mM of a non-nitrogen buffer. The tissue fixative permits the recovery of high molecular weight DNA and RNA from the tissue sample for molecular genetic analysis. The tissue fixative also preserves the morphology and immunogenicity of the tissue allowing for pathology analysis. The tissue fixative is compared to 95% alcohol and a standard fixative such as 10% BNF.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US23124794A | 1994-04-22 | 1994-04-22 | |
US08/231,247 | 1994-04-22 |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2147593A1 true CA2147593A1 (en) | 1995-10-23 |
CA2147593C CA2147593C (en) | 2008-07-29 |
Family
ID=22868391
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002147593A Expired - Fee Related CA2147593C (en) | 1994-04-22 | 1995-04-21 | Dual purpose tissue fixative |
Country Status (2)
Country | Link |
---|---|
US (1) | US5976829A (en) |
CA (1) | CA2147593C (en) |
Families Citing this family (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999009390A1 (en) * | 1997-08-20 | 1999-02-25 | The University Of Miami | A high quality, continuous throughput, tissue fixation-dehydration-fat removal-impregnation method |
CA2335747A1 (en) * | 1998-06-30 | 2000-01-06 | Lamina, Inc. | Cytological and histological fixative composition and methods of use |
EP1294853B1 (en) * | 2000-06-21 | 2010-05-05 | QIAGEN Gaithersburg, Inc. | Universal collection medium |
JP4176021B2 (en) * | 2002-03-05 | 2008-11-05 | セルメディシン株式会社 | Solid tissue immune adjuvant |
US7138226B2 (en) * | 2002-05-10 | 2006-11-21 | The University Of Miami | Preservation of RNA and morphology in cells and tissues |
US7482116B2 (en) | 2002-06-07 | 2009-01-27 | Dna Genotek Inc. | Compositions and methods for obtaining nucleic acids from sputum |
EP1676117B1 (en) * | 2003-10-24 | 2009-09-09 | The University Of Miami | Simplified tissue processing |
WO2007016935A1 (en) | 2005-07-29 | 2007-02-15 | Histogenex Nv | Methods, reagents and instrumentation for preparing impregnated tissue samples suitable for histopathological and molecular studies |
AU2009307661B2 (en) * | 2008-10-21 | 2014-11-20 | Thomas M. Donndelinger | Methods and compositions for preventing artifacts in tissue samples |
US20130095473A1 (en) | 2010-06-14 | 2013-04-18 | Qiagen Gmbh | Method for determination of target cells or tissue for extraction of biomolecules from fixed biological samples |
KR101133997B1 (en) | 2011-04-06 | 2012-04-05 | (주)셀트라존 | A composite for the use of cytological analysis |
US9442046B2 (en) | 2011-06-19 | 2016-09-13 | Abogen, Inc. | Device for sample collection |
US9835527B2 (en) | 2015-12-30 | 2017-12-05 | Sakura Finetek U.S.A., Inc. | Tissue processing reagent |
US10107727B2 (en) | 2015-12-30 | 2018-10-23 | Sakura Finetek U.S.A., Inc. | Tissue processing reagent |
FI3568475T3 (en) | 2017-01-16 | 2023-05-15 | Spectrum Solutions L L C | Nucleic acid preservation solution and methods of use |
US11712692B2 (en) | 2018-11-20 | 2023-08-01 | Spectrum Solutions L.L.C. | Sample collection system including sealing cap and valve |
US11701094B2 (en) | 2019-06-20 | 2023-07-18 | Spectrum Solutions L.L.C. | Sample collection system including valve and plug assemblies |
EP4145105A1 (en) | 2021-09-06 | 2023-03-08 | Anacyte Laboratories GmbH | Cell asservation solution |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4888278A (en) * | 1985-10-22 | 1989-12-19 | University Of Massachusetts Medical Center | In-situ hybridization to detect nucleic acid sequences in morphologically intact cells |
US5389549A (en) * | 1987-05-29 | 1995-02-14 | Toa Medical Electronics Co., Ltd. | Method for classifying leukocytes and a reagent used therefor |
US4857300A (en) * | 1987-07-27 | 1989-08-15 | Cytocorp, Inc. | Cytological and histological fixative formulation and methods for using same |
US4826691A (en) * | 1987-08-03 | 1989-05-02 | Berkley, Inc. | Carrier for fish attractant |
JPH068818B2 (en) * | 1987-11-27 | 1994-02-02 | サクラ精機株式会社 | Fixative for biopsy |
US5196182A (en) * | 1991-05-08 | 1993-03-23 | Streck Laboratories, Inc. | Tissue fixative |
US5357977A (en) * | 1993-04-23 | 1994-10-25 | St. Mary's Hospital And Medical Center, Inc. | Cytological sampling method and device |
US5405606A (en) * | 1993-12-03 | 1995-04-11 | Efh, Inc. | Embalming composition and method |
-
1995
- 1995-04-21 CA CA002147593A patent/CA2147593C/en not_active Expired - Fee Related
-
1996
- 1996-08-30 US US08/708,056 patent/US5976829A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
US5976829A (en) | 1999-11-02 |
CA2147593C (en) | 2008-07-29 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |
Effective date: 20140422 |