CA2163524A1 - Assay and reagents for identifying anti-proliferative agents - Google Patents

Assay and reagents for identifying anti-proliferative agents

Info

Publication number
CA2163524A1
CA2163524A1 CA002163524A CA2163524A CA2163524A1 CA 2163524 A1 CA2163524 A1 CA 2163524A1 CA 002163524 A CA002163524 A CA 002163524A CA 2163524 A CA2163524 A CA 2163524A CA 2163524 A1 CA2163524 A1 CA 2163524A1
Authority
CA
Canada
Prior art keywords
cell
cycle
reagents
agent
mitotic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002163524A
Other languages
French (fr)
Other versions
CA2163524C (en
Inventor
Giulio Draetta
Guillaume Cottarel
Veronique Damagnez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
MITOTIX Inc
Original Assignee
Giulio Draetta
Guillaume Cottarel
Veronique Damagnez
Mitotix, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Giulio Draetta, Guillaume Cottarel, Veronique Damagnez, Mitotix, Inc. filed Critical Giulio Draetta
Publication of CA2163524A1 publication Critical patent/CA2163524A1/en
Application granted granted Critical
Publication of CA2163524C publication Critical patent/CA2163524C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

Abstract

The present invention makes available assays and reagents for identifying antiproliferative agents, such as mitotic and meiotic inhibitors. The present assay provides a simple and rapid screening test which relies on scoring for positive cellular proliferation as indicative of anti-mitotic or anti-meiotic activity, and comprises contacting a candidate agent with a cell which has an impaired cell-cycle checkpoint and measuring the level of proliferation in the presence and absence of the agent. The checkpoint impairment is such that it either causes premature progression of the cell through at least a portion of a cell-cycle or inhibition of normal progression of the cell through at least a portion of a cell-cycle, but can be off-set by the action of an agent which inhibits at least one regulatory protein of the cell cycle (e.g., cdc25) in a manner which counter-balances the effect of the impairment.
CA002163524A 1993-06-04 1994-06-06 Assay and reagents for identifying anti-proliferative agents Expired - Fee Related CA2163524C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/073,383 US5443962A (en) 1993-06-04 1993-06-04 Methods of identifying inhibitors of cdc25 phosphatase
US073,383 1993-06-04
PCT/US1994/006365 WO1994028914A1 (en) 1993-06-04 1994-06-06 Assay and reagents for identifying anti-proliferative agents

Publications (2)

Publication Number Publication Date
CA2163524A1 true CA2163524A1 (en) 1994-12-22
CA2163524C CA2163524C (en) 2003-04-08

Family

ID=22113378

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002163524A Expired - Fee Related CA2163524C (en) 1993-06-04 1994-06-06 Assay and reagents for identifying anti-proliferative agents

Country Status (8)

Country Link
US (2) US5443962A (en)
EP (1) EP0708653B1 (en)
JP (1) JPH08510650A (en)
AT (1) ATE217190T1 (en)
AU (1) AU702174B2 (en)
CA (1) CA2163524C (en)
DE (1) DE69430573T2 (en)
WO (1) WO1994028914A1 (en)

Families Citing this family (43)

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US5443962A (en) * 1993-06-04 1995-08-22 Mitotix, Inc. Methods of identifying inhibitors of cdc25 phosphatase
AUPM516994A0 (en) * 1994-04-20 1994-05-12 Gene Shears Pty. Limited An in vivo gene expression system
US6037136A (en) * 1994-10-24 2000-03-14 Cold Spring Harbor Laboratory Interactions between RaF proto-oncogenes and CDC25 phosphatases, and uses related thereto
US5801015A (en) * 1995-06-05 1998-09-01 Mitotix, Inc. Nucleic acid encoding a Candida cell cycle regulatory protein, TYP1 polypeptide
NO953680D0 (en) * 1995-09-18 1995-09-18 Hans Prydz Cell cycle Enzymes
US7125677B2 (en) * 1995-11-13 2006-10-24 The Salk Institute For Biological Studies NIMA interacting proteins
US5972697A (en) * 1995-11-13 1999-10-26 The Salk Institute For Biological Studies NIMA interacting proteins
US5861249A (en) * 1996-04-23 1999-01-19 Cold Spring Harbor Laboratory Assays and reagents for identifying modulators of cdc25-mediated mitotic activation
US6048693A (en) * 1996-10-16 2000-04-11 Bittech, Inc. Phenotypic assays of cyclin/cyclin-dependent kinase function
CA2269633A1 (en) * 1996-10-30 1998-05-07 National Research Council Of Canada Candida albicans proteins associated with virulence and hyphal formation and uses thereof
ATE271130T1 (en) 1997-02-07 2004-07-15 Univ Princeton MODIFIED PROTEIN KINASES THAT CAN USE MODIFIED NUCLEOTIDE TRIPHOSPHATE SUBSTRATES
US6227658B1 (en) * 1997-06-23 2001-05-08 Kabushiki Kaisha Toshiba Apparatus and method for forming thin film using ink-jet mechanism
GB9718913D0 (en) * 1997-09-05 1997-11-12 Glaxo Group Ltd Substituted oxindole derivatives
CA2216559A1 (en) * 1997-09-25 1999-03-25 Michel Roberge G2 checkpoint inhibitors and assay
GB9722320D0 (en) * 1997-10-22 1997-12-17 Janssen Pharmaceutica Nv Human cell cycle checkpoint proteins
ID24372A (en) 1997-10-27 2000-07-13 Agouron Pharma COMPOUNDS OF 4-AMINO-TIAZOL-2-IL COMPOUNDS AS CDKERS
AU2107199A (en) 1998-01-08 1999-07-26 Regents Of The University Of California, The Kinesin motor modulators derived from the marine sponge (adocia)
JP2002507427A (en) 1998-03-27 2002-03-12 ザ スクリップス リサーチ インスティテュート Kinase Weel fusion protein composition, nucleotide sequence, expression system and use thereof
DE19860834C1 (en) 1998-12-30 2000-08-24 Albrecht E Sippel Method for cellular high-throughput detection of nuclear receptor-ligand interactions
US6492398B1 (en) 1999-03-04 2002-12-10 Smithkline Beechman Corporation Thiazoloindolinone compounds
US6624171B1 (en) * 1999-03-04 2003-09-23 Smithkline Beecham Corporation Substituted aza-oxindole derivatives
GB9904933D0 (en) 1999-03-04 1999-04-28 Glaxo Group Ltd Compounds
US7314926B1 (en) * 1999-04-08 2008-01-01 Antisoma Research Limited Antiproliferative activity of g-rich oligonucleotides and method of using same to bind to nucleolin
US20080318889A1 (en) * 1999-04-08 2008-12-25 Antisoma Research Limited Antiproliferative activity of G-rich oligonucleotides and method of using same to bind to nucleolin
US7960540B2 (en) * 1999-04-08 2011-06-14 Advanced Cancer Therapeutics, Llc Antiproliferative activity of G-rich oligonucleotides and method of using same to bind to nucleolin
US20080318890A1 (en) * 1999-04-08 2008-12-25 Antisoma Research Limited Antiproliferative activity of G-rich oligonucleotides and method of using same to bind to nucleolin
US8114850B2 (en) * 1999-04-08 2012-02-14 Advanced Cancer Therapeutics, Llc Antiproliferative activity of G-rich oligonucleotides and method of using same to bind to nucleolin
US6610483B1 (en) * 1999-07-23 2003-08-26 Princeton University Methods for identifying cellular responses attributable to signaling molecule inhibition and inhibitors thereof
WO2001009373A2 (en) * 1999-07-30 2001-02-08 Basf Bioresearch Corporation Native cdc25 substrates, compositions and uses related thereto
EP1259640A2 (en) * 1999-09-13 2002-11-27 Basf Aktiengesellschaft Methods and compositions for the screening of cell cycle modulators
JP4610828B2 (en) * 1999-09-22 2011-01-12 株式会社 キャンバス Compositions and methods for inhibition of G2 phase cell cycle arrest and cell sensitization to DNA damaging agents
FR2815347A1 (en) * 1999-12-14 2002-04-19 Sod Conseils Rech Applic New fusion protein of maltose-binding protein and Cdc phosphatase, useful for identifying phosphatase modulators for regulating the cell cycle
US6620818B1 (en) 2000-03-01 2003-09-16 Smithkline Beecham Corporation Method for reducing the severity of side effects of chemotherapy and/or radiation therapy
US6569853B1 (en) * 2000-11-06 2003-05-27 Combinatorx, Incorporated Combinations of chlorpromazine and pentamidine for the treatment of neoplastic disorders
US20050054708A1 (en) * 2003-07-28 2005-03-10 Nichols Matthew James Combinations of drugs for the treatment of neoplasms
AR047938A1 (en) * 2003-08-25 2006-03-15 Combinatorx Inc FORMULATIONS, CONJUGATES AND COMBINATIONS OF PHARMACOS FOR THE TREATMENT OF NEOPLASMS
KR100560701B1 (en) 2003-11-03 2006-03-16 씨제이 주식회사 Method of screening for cdc25B inhibitor using Cdc25B phosphatase-overexpression inducible cell
US20050158320A1 (en) * 2003-11-12 2005-07-21 Nichols M. J. Combinations for the treatment of proliferative diseases
US20050100508A1 (en) * 2003-11-12 2005-05-12 Nichols M. J. Methods for identifying drug combinations for the treatment of proliferative diseases
WO2007127002A2 (en) * 2006-03-31 2007-11-08 Cell Signaling Technology, Inc. Protein markers of responsiveness to type iii receptor tyrosine kinase inhibitors
US20090131351A1 (en) * 2007-11-16 2009-05-21 Antisoma Research Limited Methods, compositions, and kits for modulating tumor cell proliferation
KR100887246B1 (en) 2007-11-22 2009-03-06 건국대학교 산학협력단 Method for screening inhibitor of cell cycle regulator using caenorhabditis elegans
CN114958609B (en) * 2022-05-11 2023-10-20 西南民族大学 Methods for modulating DNA replication or repair

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06507787A (en) * 1991-05-16 1994-09-08 コールド スプリング ハーバー ラボラトリー D-type cyclins and related uses
EP0604560B1 (en) * 1991-09-20 2004-08-11 Fred Hutchinson Cancer Research Center Human cyclin e
US5262409A (en) * 1991-10-11 1993-11-16 Fred Hutchinson Cancer Research Center Binary tumor therapy
US5441880A (en) * 1991-11-18 1995-08-15 Cold Spring Harbor Laboratory Human cdc25 genes, encoded products and uses thereof
US5294538A (en) * 1991-11-18 1994-03-15 Cold Spring Harbor Labs. Method of screening for antimitotic compounds using the CDC25 tyrosine phosphatase
WO1993019176A1 (en) * 1992-03-23 1993-09-30 Imperial Cancer Research Technology Limited Leucine zippers
US5443962A (en) * 1993-06-04 1995-08-22 Mitotix, Inc. Methods of identifying inhibitors of cdc25 phosphatase

Also Published As

Publication number Publication date
US5443962A (en) 1995-08-22
AU7055194A (en) 1995-01-03
DE69430573T2 (en) 2002-11-07
AU702174B2 (en) 1999-02-18
JPH08510650A (en) 1996-11-12
EP0708653A1 (en) 1996-05-01
EP0708653B1 (en) 2002-05-08
EP0708653A4 (en) 1998-11-18
CA2163524C (en) 2003-04-08
WO1994028914A1 (en) 1994-12-22
DE69430573D1 (en) 2002-06-13
US6251585B1 (en) 2001-06-26
ATE217190T1 (en) 2002-05-15

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