CA2182906A1 - Surfaces hautement specifiques pour reactions biologiques, procede pour leur preparation et procede pour leur utilisation - Google Patents

Surfaces hautement specifiques pour reactions biologiques, procede pour leur preparation et procede pour leur utilisation

Info

Publication number
CA2182906A1
CA2182906A1 CA002182906A CA2182906A CA2182906A1 CA 2182906 A1 CA2182906 A1 CA 2182906A1 CA 002182906 A CA002182906 A CA 002182906A CA 2182906 A CA2182906 A CA 2182906A CA 2182906 A1 CA2182906 A1 CA 2182906A1
Authority
CA
Canada
Prior art keywords
highly specific
biological reactions
utilization
preparation
layer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002182906A
Other languages
English (en)
Other versions
CA2182906C (fr
Inventor
David Bensimon
Aaron Bensimon
Francois Heslot
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Institut Pasteur de Lille
Original Assignee
David Bensimon
Aaron Bensimon
Francois Heslot
Institut Pasteur
Centre National De La Recherche Scientifique (Cnrs)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26230948&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2182906(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from FR9401574A external-priority patent/FR2716206B1/fr
Application filed by David Bensimon, Aaron Bensimon, Francois Heslot, Institut Pasteur, Centre National De La Recherche Scientifique (Cnrs) filed Critical David Bensimon
Publication of CA2182906A1 publication Critical patent/CA2182906A1/fr
Application granted granted Critical
Publication of CA2182906C publication Critical patent/CA2182906C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00387—Applications using probes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/0054—Means for coding or tagging the apparatus or the reagents
    • B01J2219/00572—Chemical means
    • B01J2219/00576—Chemical means fluorophore
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00608—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/0061—The surface being organic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/00626—Covalent
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/00628—Ionic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/0063—Other, e.g. van der Waals forces, hydrogen bonding
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00632—Introduction of reactive groups to the surface
    • B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00677—Ex-situ synthesis followed by deposition on the substrate
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/969—Multiple layering of reactants
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/97—Test strip or test slide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
    • Y10S530/811—Peptides or proteins is immobilized on, or in, an inorganic carrier
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/145555—Hetero-N

Abstract

La présente invention concerne notamment une surface hautement spécifique pour réactions biologiques, caractérisée en ce qu'elle comporte un support présentant en surface au moins une couche essentiellement compacte d'un composé organique présentant, à l'extérieur de la couche, un groupement exposé comportant une double liaison éthylénique ayant une affinité pour un type de molécule à activité biologique dans certaines conditions de réaction, les autres éléments de la couche étant essentiellement non accessibles pour lesdites molécules dans lesdites conditions de réaction.
CA002182906A 1994-02-11 1995-02-10 Surfaces hautement specifiques pour reactions biologiques, procede pour leur preparation et procede pour leur utilisation Expired - Lifetime CA2182906C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
FR9401574A FR2716206B1 (fr) 1994-02-11 1994-02-11 Surfaces hautement spécifiques pour réactions biologiques, procédé pour leur préparation et procédé de dosage d'une molécule utilisant ces surfaces.
FR94/07444 1994-06-17
FR9407444A FR2716263B1 (fr) 1994-02-11 1994-06-17 Procédé d'alignement de macromolécules par passage d'un ménisque et applications dans un procédé de mise en évidence, séparation et/ou dosage d'une macromolécule dans un échantillon.
FR94/01574 1994-06-17
PCT/FR1995/000164 WO1995022056A1 (fr) 1994-02-11 1995-02-10 Surfaces hautement specifiques pour reactions biologiques, procede pour leur preparation et procede pour leur utilisation

Publications (2)

Publication Number Publication Date
CA2182906A1 true CA2182906A1 (fr) 1995-08-17
CA2182906C CA2182906C (fr) 2006-08-15

Family

ID=26230948

Family Applications (2)

Application Number Title Priority Date Filing Date
CA002182906A Expired - Lifetime CA2182906C (fr) 1994-02-11 1995-02-10 Surfaces hautement specifiques pour reactions biologiques, procede pour leur preparation et procede pour leur utilisation
CA2182905A Expired - Lifetime CA2182905C (fr) 1994-02-11 1995-02-10 Procede d'alignement de macromolecules par passage d'un menisque et applications

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2182905A Expired - Lifetime CA2182905C (fr) 1994-02-11 1995-02-10 Procede d'alignement de macromolecules par passage d'un menisque et applications

Country Status (15)

Country Link
US (11) US6265153B1 (fr)
EP (3) EP1369494A3 (fr)
JP (2) JP3741718B2 (fr)
KR (2) KR100424939B1 (fr)
CN (2) CN1088110C (fr)
AT (2) ATE248927T1 (fr)
AU (2) AU699136B2 (fr)
CA (2) CA2182906C (fr)
DE (2) DE69531667T2 (fr)
DK (2) DK0744028T3 (fr)
ES (2) ES2206494T3 (fr)
FR (1) FR2716263B1 (fr)
NZ (2) NZ279861A (fr)
PT (2) PT743988E (fr)
WO (2) WO1995022056A1 (fr)

Families Citing this family (121)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2716263B1 (fr) 1994-02-11 1997-01-17 Pasteur Institut Procédé d'alignement de macromolécules par passage d'un ménisque et applications dans un procédé de mise en évidence, séparation et/ou dosage d'une macromolécule dans un échantillon.
US8142708B2 (en) * 1995-04-03 2012-03-27 Wisconsin Alumni Research Foundation Micro fluidic system for single molecule imaging
US7775368B2 (en) * 1995-04-03 2010-08-17 Wisconsin Alumni Research Foundation Micro-channel long molecule manipulation system
FR2737574B1 (fr) * 1995-08-03 1997-10-24 Pasteur Institut Appareillage d'alignement parallele de macromolecules et utilisation
DE69637317T2 (de) 1995-08-09 2008-10-16 Simon, Adam J. Molekulare ausrichtung von makromolekuelen mit hilfe eines fluessigkeitsmeniskus auf einer hochspezifischen oberflaeche
NZ320546A (en) * 1995-11-13 1999-01-28 Pasteur Institut Ultrahigh resolution comparative nucleic acid hybridization to combed dna fibers
FR2755147B1 (fr) * 1996-10-30 1999-01-15 Pasteur Institut Procede de diagnostic de maladies genetiques par peignage moleculaire et coffret de diagnostic
FR2755149B1 (fr) 1996-10-30 1999-01-15 Pasteur Institut Procede de diagnostic de maladies genetiques par peignage moleculaire et coffret de diagnostic
US5830665A (en) * 1997-03-03 1998-11-03 Exact Laboratories, Inc. Contiguous genomic sequence scanning
WO1998041651A1 (fr) * 1997-03-18 1998-09-24 Hsc Research & Development Limited Partnership Procede de preparation de chromatine
US6893877B2 (en) 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
EP1714699B1 (fr) * 1998-01-12 2010-08-18 Massachusetts Institute of Technology Systeme d' analyse d' une pluralite d' échantillons
US6114466A (en) * 1998-02-06 2000-09-05 Renal Tech International Llc Material for purification of physiological liquids of organism
US20030022207A1 (en) * 1998-10-16 2003-01-30 Solexa, Ltd. Arrayed polynucleotides and their use in genome analysis
CA2339121A1 (fr) * 1998-07-30 2000-02-10 Shankar Balasubramanian Biomolecules en rangees et leur utilisation dans une procedure de sequencage
US20100130368A1 (en) * 1998-07-30 2010-05-27 Shankar Balasubramanian Method and system for sequencing polynucleotides
US20040106110A1 (en) * 1998-07-30 2004-06-03 Solexa, Ltd. Preparation of polynucleotide arrays
DE19853640C2 (de) * 1998-11-20 2002-01-31 Molecular Machines & Ind Gmbh Mehrgefäßanordnung mit verbesserter Empfindlichkeit für die optische Analytik, Verfahren zu ihrer Herstellung sowie ihre Verwendung in optischen Analyseverfahren
CA2367912A1 (fr) * 1999-03-19 2000-09-28 Genencor International, Inc. Plaque d'essais a trous traversants multiples pour criblage a haut debit
US20020042081A1 (en) * 2000-10-10 2002-04-11 Eric Henderson Evaluating binding affinities by force stratification and force panning
US6573369B2 (en) * 1999-05-21 2003-06-03 Bioforce Nanosciences, Inc. Method and apparatus for solid state molecular analysis
US20030073250A1 (en) * 1999-05-21 2003-04-17 Eric Henderson Method and apparatus for solid state molecular analysis
US20030186311A1 (en) * 1999-05-21 2003-10-02 Bioforce Nanosciences, Inc. Parallel analysis of molecular interactions
US6248537B1 (en) 1999-05-28 2001-06-19 Institut Pasteur Use of the combing process for the identification of DNA origins of replication
US6692914B1 (en) 1999-07-02 2004-02-17 Symyx Technologies, Inc. Polymer brushes for immobilizing molecules to a surface or substrate, where the polymers have water-soluble or water-dispersible segments and probes bonded thereto
US6762059B2 (en) * 1999-08-13 2004-07-13 U.S. Genomics, Inc. Methods and apparatuses for characterization of single polymers
US6696022B1 (en) 1999-08-13 2004-02-24 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
US6927065B2 (en) 1999-08-13 2005-08-09 U.S. Genomics, Inc. Methods and apparatus for characterization of single polymers
US6319674B1 (en) * 1999-09-16 2001-11-20 Agilent Technologies, Inc. Methods for attaching substances to surfaces
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
WO2001061054A2 (fr) 2000-02-18 2001-08-23 Board Of Trustees Of The Leland Stanford Junior University Appareils et procedes de conduite en parallele de reactions sur des microvolumes
US6897015B2 (en) * 2000-03-07 2005-05-24 Bioforce Nanosciences, Inc. Device and method of use for detection and characterization of pathogens and biological materials
ATE402760T1 (de) * 2000-08-15 2008-08-15 Bioforce Nanosciences Inc Vorrichtung zur bildung von nanomolekularen netzwerken
EP3034627B1 (fr) 2000-10-06 2019-01-30 The Trustees of Columbia University in the City of New York Procédé massivement parallèle pour décoder l'adn et l'arn
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US7205161B2 (en) * 2001-01-10 2007-04-17 Symyx Technologies, Inc. Polymer brushes for immobilizing molecules to a surface or substrate having improved stability
US6528117B2 (en) 2001-01-19 2003-03-04 Paul Lewis Method for coating a substance on one side of a substrate using a single miniscus
DE60222628T2 (de) † 2001-01-30 2008-07-17 Solexa Ltd., Saffron Walden Herstellung von matrizen aus polynukleotiden
DK1370690T3 (da) * 2001-03-16 2012-07-09 Kalim Mir Arrays og fremgangsmåder til anvendelse heraf
US10272409B2 (en) * 2001-07-11 2019-04-30 Michael E. Hogan Methods and devices based upon a novel form of nucleic acid duplex on a surface
WO2003006676A2 (fr) 2001-07-13 2003-01-23 Nanosphere, Inc. Methode d'immobilisation de molecules sur des surfaces
US7297553B2 (en) * 2002-05-28 2007-11-20 Nanosphere, Inc. Method for attachment of silylated molecules to glass surfaces
CA2563510A1 (fr) * 2001-07-13 2005-11-17 Nanosphere, Inc. Procede pour la preparation de substrats comportant des molecules immobilisees et substrats
AU2002329606A1 (en) * 2001-07-17 2003-03-03 Bioforce Nanosciences, Inc. Combined molecular blinding detection through force microscopy and mass spectrometry
US20030044798A1 (en) * 2001-08-31 2003-03-06 Lefkowitz Steven M. Methods for generating ligand arrays via deposition of ligands onto olefin displaying substrates, and arrays produced thereby
US6916541B2 (en) 2001-09-07 2005-07-12 Penn State Research Foundation Modified substrates for the attachment of biomolecules
JP2005504698A (ja) * 2001-09-07 2005-02-17 ボーデン ケミカル インコーポレイテッド 接着促進剤を用いた被覆光ファイバと、その製造方法及び使用方法
US7042488B2 (en) 2001-09-27 2006-05-09 Fujinon Corporation Electronic endoscope for highlighting blood vessel
US20050019488A1 (en) * 2001-11-30 2005-01-27 Cambridge Polymer Group, Inc., Boston, Ma Layered aligned polymer structures and methods of making same
US7048963B2 (en) * 2001-11-30 2006-05-23 Cambridge Polymers Group, Inc. Layered aligned polymer structures and methods of making same
US20030162181A1 (en) * 2002-02-28 2003-08-28 Eastman Kodak Company DNA sequencing and gene identification
US7094537B2 (en) * 2002-04-30 2006-08-22 Agilent Technologies, Inc. Micro arrays with structured and unstructured probes
EP1540017A4 (fr) * 2002-05-09 2006-05-24 Us Genomics Inc Methodes d'analyse d'un acide nucleique
US20050239193A1 (en) * 2002-05-30 2005-10-27 Bioforce Nanosciences, Inc. Device and method of use for detection and characterization of microorganisms and microparticles
US20040031167A1 (en) * 2002-06-13 2004-02-19 Stein Nathan D. Single wafer method and apparatus for drying semiconductor substrates using an inert gas air-knife
US20030232916A1 (en) * 2002-06-14 2003-12-18 Lorah Dennis Paul Nonaqueous compositions
US6952651B2 (en) * 2002-06-17 2005-10-04 Intel Corporation Methods and apparatus for nucleic acid sequencing by signal stretching and data integration
AU2003251986A1 (en) * 2002-07-17 2004-02-02 U.S.Genomics, Inc. Methods and compositions for analyzing polymers using chimeric tags
US8277753B2 (en) 2002-08-23 2012-10-02 Life Technologies Corporation Microfluidic transfer pin
ATE395970T1 (de) * 2002-09-17 2008-06-15 Kalachev Alexey Verfahren zum anordnen einem polymer-molekül
US7361821B2 (en) * 2002-09-20 2008-04-22 Intel Corporation Controlled alignment of nanobarcodes encoding specific information for scanning probe microscopy (SPM) reading
AU2003278852A1 (en) * 2002-09-20 2004-05-13 Intel Corporation Controlled alignment of nano-barcodes encoding specific information for scanning probe microscopy (spm) reading
US7606403B2 (en) 2002-10-17 2009-10-20 Intel Corporation Model-based fusion of scanning probe microscopic images for detection and identification of molecular structures
US7682565B2 (en) 2002-12-20 2010-03-23 Biotrove, Inc. Assay apparatus and method using microfluidic arrays
WO2004060044A2 (fr) * 2003-01-02 2004-07-22 Bioforce Nanosciences, Inc. Methode et appareil pour une analyse moleculaire dans de petits volumes d'echantillon
US6861473B2 (en) * 2003-02-28 2005-03-01 Baxter International Inc. Macromolecular ketoaldehydes
US20050123944A1 (en) * 2003-08-01 2005-06-09 U.S. Genomics, Inc. Methods and compositions related to the use of sequence-specific endonucleases for analyzing nucleic acids under non-cleaving conditions
EP1516665A1 (fr) * 2003-09-18 2005-03-23 Sony International (Europe) GmbH Méthode d'immobilisation et d'étirage d'un acide nucléique sur un substrat
US20050064435A1 (en) * 2003-09-24 2005-03-24 Xing Su Programmable molecular barcodes
US20050136413A1 (en) * 2003-12-22 2005-06-23 Briggs Michael W. Reagent systems for biological assays
DE10361075A1 (de) * 2003-12-22 2005-07-28 Pac Tech - Packaging Technologies Gmbh Verfahren und Vorichtung zur Trocknung von Schaltungssubstraten
US20050147976A1 (en) 2003-12-29 2005-07-07 Xing Su Methods for determining nucleotide sequence information
US20050151126A1 (en) * 2003-12-31 2005-07-14 Intel Corporation Methods of producing carbon nanotubes using peptide or nucleic acid micropatterning
CA2559171A1 (fr) 2004-03-12 2005-09-29 Biotrove, Inc. Chargement d'ensembles d'echantillons de l'ordre du nanolitre
US20050221408A1 (en) * 2004-03-19 2005-10-06 U.S. Genomics, Inc. Compositions and methods for detection of single molecules
AU2005275061B2 (en) * 2004-07-14 2012-05-24 Zs Genetics, Inc. Systems and methods of analyzing nucleic acid polymers and related components
US20060105453A1 (en) 2004-09-09 2006-05-18 Brenan Colin J Coating process for microfluidic sample arrays
US20060134679A1 (en) * 2004-12-17 2006-06-22 U.S. Genomics, Inc. Methods and compositions for acquiring information from unstretched polymer conformations
US7736818B2 (en) 2004-12-27 2010-06-15 Inphase Technologies, Inc. Holographic recording medium and method of making it
US20060223071A1 (en) * 2005-04-01 2006-10-05 Wisniewski Michele E Methods, compositions, and kits for detecting nucleic acids in a single vessel
US7319762B2 (en) * 2005-08-23 2008-01-15 Andrea Electronics Corporation Headset with flashing light emitting diodes
US8566038B2 (en) * 2005-10-21 2013-10-22 The Regents Of The University Of California Compositions and methods for analyzing immobilized nucleic acids
US20070178503A1 (en) * 2005-12-19 2007-08-02 Feng Jiang In-situ genomic DNA chip for detection of cancer
US20100015607A1 (en) * 2005-12-23 2010-01-21 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
AU2006330834B2 (en) 2005-12-23 2013-09-12 Nanostring Technologies, Inc. Compositions comprising oriented, immobilized macromolecules and methods for their preparation
CA2653095C (fr) * 2006-05-22 2013-07-16 Nanostring Technologies, Inc. Systeme et procedes pour analyser des nanoreporteurs
US7312029B1 (en) * 2006-06-30 2007-12-25 Searete Llc Method of combing an elongated molecule
WO2008056816A1 (fr) * 2006-11-07 2008-05-15 Kyoto University Support de suspension pour une molécule d'acide nucléique linéaire, procédé d'extension d'une molécule d'acide nucléique linéaire et spécimen de molécule d'acide nucléique linéaire
WO2008069973A2 (fr) 2006-12-01 2008-06-12 The Trustees Of Columbia University In The City Of New York Séquençage en quatre couleurs de l'adn par synthèse utilisant des terminateurs nucléotidiques réversibles, fluorescents et clivables
JP5555157B2 (ja) * 2007-04-10 2014-07-23 ナノストリング テクノロジーズ， インコーポレイテッド ナノレポーターにおける使用のための標的特異的配列を同定するための方法およびコンピュータシステム
US8951731B2 (en) * 2007-10-15 2015-02-10 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
US20110014611A1 (en) * 2007-10-19 2011-01-20 Jingyue Ju Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequences by synthesis
US9115163B2 (en) 2007-10-19 2015-08-25 The Trustees Of Columbia University In The City Of New York DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
JP5578596B2 (ja) * 2008-08-04 2014-08-27 独立行政法人物質・材料研究機構 高分子ナノワイヤの製造方法
EP2175037B1 (fr) 2008-09-26 2017-10-11 Genomic Vision Procédé pour analyser les réseaux de répétitions en tandem du D4Z4 d'un acide nucléique et kit correspondant
US8709717B2 (en) 2009-04-03 2014-04-29 Illumina, Inc. Generation of uniform fragments of nucleic acids using patterned substrates
WO2010144128A2 (fr) * 2009-06-08 2010-12-16 Zs Genetics, Inc. Alignement moléculaire et fixation de molécule d'acide nucléique
JP2013524806A (ja) 2010-04-23 2013-06-20 ゲノミク ビジョン 分子コーミングを用いるゲノムｄｎａ及び感染性ウイルスｄｎａの検出によるウイルス感染の診断
WO2012038831A2 (fr) 2010-09-24 2012-03-29 Genomic Vision Procédé de détection, de quantification et de cartographie d'un dommage et/ou d'une réparation de brins d'adn
FR2966844A1 (fr) 2010-11-03 2012-05-04 Vivatech Procede d'analyse genomique
US20140121132A1 (en) * 2011-03-11 2014-05-01 Cornell University Systems and methods for high resolution biomolecular imaging and analysis
EP2714931A1 (fr) 2011-06-03 2014-04-09 Genomic Vision Évaluation du risque de cancer basée sur la séquence cnv rnu2 et sur l'interaction entre cnv rnu2 et brca1
WO2013087789A1 (fr) 2011-12-13 2013-06-20 Glykos Finland Ltd. Réseaux d'isoformes d'anticorps et procédés associés
EP3495499A1 (fr) 2011-12-30 2019-06-12 Quest Diagnostics Investments Incorporated Analyse d'acides nucléiques au moyen d'une émulsion pcr
EP2805281A4 (fr) * 2012-01-18 2015-09-09 Singular Bio Inc Procédés de cartographie de molécules à code-barres destinés à la détection et au séquençage d'une variation structurale
US9028776B2 (en) 2012-04-18 2015-05-12 Toxic Report Llc Device for stretching a polymer in a fluid sample
WO2014078652A1 (fr) 2012-11-16 2014-05-22 Zs Genetics, Inc. Nucléosides, nucléotides et polymères d'acides nucléiques marqués avec des atomes lourds et leurs utilisations
JP6475171B2 (ja) * 2013-02-05 2019-02-27 バイオナノ ジェノミクス、 インコーポレイテッド 単分子解析方法
US20160040220A1 (en) 2013-03-15 2016-02-11 Genomic Vision Methods for the detection of breakpoints in rearranged genomic sequences
WO2014144883A1 (fr) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Molecules marquees par des amas de raman destinees a l'imagerie biologique
US10036071B2 (en) 2013-03-15 2018-07-31 Genomic Vision Methods for the detection of sequence amplification in the BRCA1 locus
JP2016521575A (ja) 2013-06-14 2016-07-25 ナノストリング テクノロジーズ，インコーポレイティド 多重化可能なタグベースのレポーターシステム
WO2017075179A1 (fr) 2015-10-27 2017-05-04 Zs Genetics, Inc. Séquençage par déconvolution
CN110168102A (zh) 2016-11-15 2019-08-23 基因组影像公司 利用分子梳理监测经修饰的核酸酶诱导的基因编辑事件的方法
EP3548637A1 (fr) 2016-11-29 2019-10-09 Genomic Vision Procédé de conception d'un ensemble de séquences polynucléotidiques destiné à l'analyse d'événements spécifiques dans une région génétique d'intérêt
KR102077643B1 (ko) * 2018-02-09 2020-04-07 광운대학교 산학협력단 메니스커스 곡면을 갖는 시료 내 입자 분리 장치 및 방법
EP4143333A1 (fr) 2020-04-30 2023-03-08 Dimensiongen Dispositifs et procédés pour la manipulation macromoléculaire
CN116171331A (zh) 2020-06-01 2023-05-26 德迈逊科技有限公司 用于基因组分析的装置和方法
US11802312B2 (en) 2020-08-10 2023-10-31 Dimension Genomics Inc. Devices and methods for multi-dimensional genome analysis
CN113514297A (zh) * 2021-06-21 2021-10-19 福建农林大学 一种用于单分子dna拉伸的磁控分子梳方法
WO2023055776A1 (fr) 2021-09-29 2023-04-06 Michael David Austin Dispositifs et procédés d'interrogation de macromolécules

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4278651A (en) * 1978-09-27 1981-07-14 Becton Dickinson & Company Supported receptor and use thereof in an assay
NL8102178A (nl) * 1981-05-02 1982-12-01 Stichting Centraal Lab Werkwijze voor het aantonen van tegen bepaalde antigenen gerichte antistoffen, alsmede inrichting en reagentia voor het uitvoeren van deze werkwijze.
US4554088A (en) * 1983-05-12 1985-11-19 Advanced Magnetics Inc. Magnetic particles for use in separations
US4698302A (en) * 1983-05-12 1987-10-06 Advanced Magnetics, Inc. Enzymatic reactions using magnetic particles
GB8314523D0 (en) * 1983-05-25 1983-06-29 Lowe C R Diagnostic device
US4539061A (en) * 1983-09-07 1985-09-03 Yeda Research And Development Co., Ltd. Process for the production of built-up films by the stepwise adsorption of individual monolayers
US4724207A (en) * 1984-02-02 1988-02-09 Cuno Incorporated Modified siliceous chromatographic supports
US4794168A (en) * 1984-04-24 1988-12-27 Scripps Clinic And Research Foundation Leukemia-associated virus immunogen, vaccine and assay
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4803170A (en) * 1985-05-09 1989-02-07 Ultra Diagnostics Corporation Competitive immunoassay method, device and test kit
US4722890A (en) * 1985-08-27 1988-02-02 The United States Of America As Represented By The Department Of Health And Human Services Quantitative assay for human terminal complement cascade activation
US5447841A (en) * 1986-01-16 1995-09-05 The Regents Of The Univ. Of California Methods for chromosome-specific staining
US4709037A (en) * 1987-02-17 1987-11-24 Hoechst Celanese Corporation Biotinylating agents
DE3717210C2 (de) * 1987-05-22 1993-11-25 Diagen Inst Molekularbio Verfahren zur Modifizierung von Nukleinsäuren
US5098977A (en) * 1987-09-23 1992-03-24 Board Of Regents, The University Of Texas System Methods and compositions for providing articles having improved biocompatability characteristics
US4921809A (en) * 1987-09-29 1990-05-01 Findley Adhesives, Inc. Polymer coated solid matrices and use in immunoassays
FR2634023B1 (fr) * 1988-07-08 1994-03-25 Bio Merieux Reactif sous forme de support solide permettant de fixer par covalence un ligand biologique amine, sa preparation et son utilisation
US5102798A (en) * 1988-09-08 1992-04-07 Allage Associates Surface functionalized Langmuir-Blodgett films for immobilization of active moieties
US5237016A (en) * 1989-01-05 1993-08-17 Siska Diagnostics, Inc. End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids
JP2910929B2 (ja) * 1989-03-23 1999-06-23 浜松ホトニクス株式会社 磁気粒子よりなる粒子の表面改質方法
EP0391674B1 (fr) * 1989-04-05 1996-03-20 New York University Procédé de caractérisation de particules
US5240846A (en) * 1989-08-22 1993-08-31 The Regents Of The University Of Michigan Gene therapy vector for cystic fibrosis
US5232829A (en) * 1989-09-29 1993-08-03 Hoffmann-La Roche Inc. Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes
US5215882A (en) * 1989-11-30 1993-06-01 Ortho Diagnostic Systems, Inc. Method of immobilizing nucleic acid on a solid surface for use in nucleic acid hybridization assays
FR2656319B1 (fr) * 1989-12-27 1992-03-20 Rhone Poulenc Chimie Microspheres composites magnetisables a base d'un polymere organosilicie reticule, leur procede de preparation et leur application en biologie.
US5079169A (en) * 1990-05-22 1992-01-07 The Regents Of The Stanford Leland Junior University Method for optically manipulating polymer filaments
US5200315A (en) * 1990-07-25 1993-04-06 Eastman Kodak Company Particulate biologically active reagent containing polyoxyalkylene side chains, analytical element and methods for use of the reagent
US5244796A (en) * 1990-10-12 1993-09-14 Syracuse University Cloned leuconostoc mesenteroides glucose-6-phosphate dehydrogenase genes and method of making glucose-6-phospate dehydrogenase
US5474796A (en) * 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
AU4238593A (en) * 1992-05-07 1993-11-29 Ig Laboratories, Inc. Methods for high resolution gene mapping
CA2098960C (fr) * 1992-07-10 2004-11-02 Richard Barner Surfaces de liaison biospecifique sur support solide et methode de preparation
US5372930A (en) * 1992-09-16 1994-12-13 The United States Of America As Represented By The Secretary Of The Navy Sensor for ultra-low concentration molecular recognition
US5610287A (en) * 1993-12-06 1997-03-11 Molecular Tool, Inc. Method for immobilizing nucleic acid molecules
FR2716263B1 (fr) * 1994-02-11 1997-01-17 Pasteur Institut Procédé d'alignement de macromolécules par passage d'un ménisque et applications dans un procédé de mise en évidence, séparation et/ou dosage d'une macromolécule dans un échantillon.
US5624711A (en) 1995-04-27 1997-04-29 Affymax Technologies, N.V. Derivatization of solid supports and methods for oligomer synthesis
DE69637317T2 (de) 1995-08-09 2008-10-16 Simon, Adam J. Molekulare ausrichtung von makromolekuelen mit hilfe eines fluessigkeitsmeniskus auf einer hochspezifischen oberflaeche
US5554088A (en) * 1995-09-12 1996-09-10 Zlojutro; Milan Sport training bag and support
US5851769A (en) 1995-09-27 1998-12-22 The Regents Of The University Of California Quantitative DNA fiber mapping

Also Published As

Publication number Publication date
CN1088110C (zh) 2002-07-24
WO1995021939A1 (fr) 1995-08-17
DE69531666D1 (de) 2003-10-09
US7754425B2 (en) 2010-07-13
US6265153B1 (en) 2001-07-24
US20060257910A1 (en) 2006-11-16
WO1995022056A1 (fr) 1995-08-17
CN1144540A (zh) 1997-03-05
EP0744028B1 (fr) 2003-09-03
EP1369494A2 (fr) 2003-12-10
KR100424939B1 (ko) 2004-07-27
CN1144561A (zh) 1997-03-05
US5840862A (en) 1998-11-24
US6130044A (en) 2000-10-10
US20020031774A1 (en) 2002-03-14
JPH09509057A (ja) 1997-09-16
DK0743988T3 (da) 2004-01-05
JP3741718B2 (ja) 2006-02-01
ES2206493T3 (es) 2004-05-16
CN1125342C (zh) 2003-10-22
ATE249045T1 (de) 2003-09-15
AU699136B2 (en) 1998-11-26
CA2182905A1 (fr) 1995-08-17
NZ281255A (en) 1998-05-27
EP1369494A3 (fr) 2003-12-17
US5846724A (en) 1998-12-08
US7122647B2 (en) 2006-10-17
AU1712095A (en) 1995-08-29
DE69531667D1 (de) 2003-10-09
EP0743988A1 (fr) 1996-11-27
US5677126A (en) 1997-10-14
DE69531666T2 (de) 2004-07-01
US6054327A (en) 2000-04-25
DK0744028T3 (da) 2003-12-22
KR100395018B1 (ko) 2003-08-19
FR2716263A1 (fr) 1995-08-18
JP3741719B2 (ja) 2006-02-01
NZ279861A (en) 1998-07-28
US6294324B1 (en) 2001-09-25
EP0743988B1 (fr) 2003-09-03
EP0744028A1 (fr) 1996-11-27
CA2182906C (fr) 2006-08-15
PT743988E (pt) 2004-01-30
ES2206494T3 (es) 2004-05-16
US20030175779A1 (en) 2003-09-18
DE69531667T2 (de) 2004-06-24
CA2182905C (fr) 2010-08-17
FR2716263B1 (fr) 1997-01-17
ATE248927T1 (de) 2003-09-15
JPH09509056A (ja) 1997-09-16
PT744028E (pt) 2004-01-30
AU1814595A (en) 1995-08-29
US6548255B2 (en) 2003-04-15
US6303296B1 (en) 2001-10-16

Similar Documents

Publication Publication Date Title
CA2182906A1 (fr) Surfaces hautement specifiques pour reactions biologiques, procede pour leur preparation et procede pour leur utilisation
DE69430016D1 (de) Proteinreinigung
EP1398326A3 (fr) Anticorps monoclonaux contre l'enzyme chymotryptique de la couche cornée (ECCC) recombinée
AU1843395A (en) Antibody purification
CA2145467A1 (fr) Disque multimateriaux pour freinage a haute energie
ATE476192T1 (de) Ligandenbindungsstelle von rage und deren verwendungen
CA2182013A1 (fr) Anticorps humanises diriges contre la molecule d'adhesion leucocytaire vla-4
NO903762D0 (no) Fremgangsmaate for aa opprette diffusjonsbinding mellom korrosjonsbestandige materialer.
WO1999024076A3 (fr) Agents diagnostiques et therapeutiques derivatises par salicylate
WO1996016172A3 (fr) Modulateurs de la fonction des proteines d'ancrage
CA2286403A1 (fr) Presulfuration hors site en presence de molecule hydrocarbonee
AU2963995A (en) A zeolite containing composition with a selectivity enhancing coating
AU4279996A (en) New method for identifying and evaluating biologically active molecules
CA2222311A1 (fr) Utilisation d'au moins un agoniste beta-adrenergique en tant qu'antagoniste de substance p
CA2197564A1 (fr) Craquage catalytique avec mcm-49
FR2714305B1 (fr) Catalyseur pour la réduction de la teneur en benzène dans les essences.
EP0992584A3 (fr) Ligands purifiés de FLT3 de mammifères et leurs agonistes et antagonistes
ZA987913B (en) Fractionation of hemicellulosic materials.
DE69813599D1 (de) Reinigung von molekülen
WO1999056872A3 (fr) Catalyseur d'oxydation et procede d'oxydation catalytique
WO2000018803A3 (fr) Anticorps lies a un antigene mammalien de la cellule de langerhans et leurs utilisations
AU5971598A (en) Novel antibody, renin-active substance containing the same and prorenin assay reagent using the same
AU2001292118A1 (en) Dye-labelled peptide and its diagnostic use
IT8521764A0 (it) Pellicola reticolata per valutazioni quantitative o semiquantitative al microscopio su preparati biologici.
AU2715999A (en) Method for improving the operational life of supported catalysts covered with gold particles and used for oxidising unsaturated hydrocarbons

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20150210