CA2189427A1 - Subtilisin 309 variants having decreased adsorption and increased hydrolysis - Google Patents
Subtilisin 309 variants having decreased adsorption and increased hydrolysisInfo
- Publication number
- CA2189427A1 CA2189427A1 CA002189427A CA2189427A CA2189427A1 CA 2189427 A1 CA2189427 A1 CA 2189427A1 CA 002189427 A CA002189427 A CA 002189427A CA 2189427 A CA2189427 A CA 2189427A CA 2189427 A1 CA2189427 A1 CA 2189427A1
- Authority
- CA
- Canada
- Prior art keywords
- subtilisin
- variants
- loop region
- amino acid
- wild
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/22—Peroxides; Oxygen; Ozone
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/23—Sulfur; Selenium; Tellurium; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/24—Phosphorous; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/41—Amines
- A61K8/416—Quaternary ammonium compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/42—Amides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/46—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing sulfur
- A61K8/463—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing sulfur containing sulfuric acid derivatives, e.g. sodium lauryl sulfate
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/46—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing sulfur
- A61K8/466—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing sulfur containing sulfonic acid derivatives; Salts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4906—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
- A61Q11/02—Preparations for deodorising, bleaching or disinfecting dentures
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/10—Washing or bathing preparations
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06Q—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES; SYSTEMS OR METHODS SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES, NOT OTHERWISE PROVIDED FOR
- G06Q40/00—Finance; Insurance; Tax strategies; Processing of corporate or income taxes
- G06Q40/04—Trading; Exchange, e.g. stocks, commodities, derivatives or currency exchange
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/20—Chemical, physico-chemical or functional or structural properties of the composition as a whole
- A61K2800/22—Gas releasing
- A61K2800/222—Effervescent
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/86—Products or compounds obtained by genetic engineering
Abstract
The present invention relates to subtilisin (309) variants having a modified amino acid sequence of wild-type subtilisin (309) amino acid sequence, the wild-type amino acid sequence comprising a first loop region, a second loop region, a third loop region, a fourth loop region, a fifth loop region and a sixth loop region; wherein the modified amino acid sequence comprises different amino acids than that occurring in wild-type subtilisin (309) (i.e., substitution) at specifically identified positions in one or more of the loop regions whereby the subtilisin (309) variant has decreased adsorption to, and increased hydrolysis of, an insoluble substrate as compared to the wild type subtilisin (309). The present invention also relates to DNA sequence encoding such subtilisin (309) variants. The present invention also relates to compositions comprising such subtilisin (309) variants for cleaning a variety of surfaces.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US23793894A | 1994-05-02 | 1994-05-02 | |
US237,938 | 1994-05-02 | ||
US08/400,068 US6599730B1 (en) | 1994-05-02 | 1995-03-07 | Subtilisin 309 variants having decreased adsorption and increased hydrolysis |
US400,068 | 1995-03-07 | ||
PCT/US1995/004760 WO1995030011A2 (en) | 1994-05-02 | 1995-04-18 | Subtilisin 309 variants having decreased adsorption and increased hydrolysis |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2189427A1 true CA2189427A1 (en) | 1995-11-09 |
CA2189427C CA2189427C (en) | 2003-10-28 |
Family
ID=26931187
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002189427A Expired - Fee Related CA2189427C (en) | 1994-05-02 | 1995-04-18 | Subtilisin 309 variants having decreased adsorption and increased hydrolysis |
Country Status (11)
Country | Link |
---|---|
US (1) | US6599730B1 (en) |
EP (1) | EP0758389A1 (en) |
JP (1) | JPH09512434A (en) |
CN (1) | CN1151187A (en) |
AU (1) | AU2294495A (en) |
BR (1) | BR9507605A (en) |
CA (1) | CA2189427C (en) |
CZ (1) | CZ321796A3 (en) |
HU (1) | HUT75558A (en) |
MX (1) | MX9605358A (en) |
WO (1) | WO1995030011A2 (en) |
Families Citing this family (103)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6436690B1 (en) * | 1993-09-15 | 2002-08-20 | The Procter & Gamble Company | BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted |
US6440717B1 (en) * | 1993-09-15 | 2002-08-27 | The Procter & Gamble Company | BPN′ variants having decreased adsorption and increased hydrolysis |
DK1921148T3 (en) * | 1994-02-24 | 2011-09-26 | Henkel Ag & Co Kgaa | Enhanced enzymes and detergents containing these |
DE4411223A1 (en) * | 1994-03-31 | 1995-10-05 | Solvay Enzymes Gmbh & Co Kg | Use of alkaline proteases in commercial textile washing processes |
US6599730B1 (en) * | 1994-05-02 | 2003-07-29 | Procter & Gamble Company | Subtilisin 309 variants having decreased adsorption and increased hydrolysis |
US6455295B1 (en) * | 1995-03-08 | 2002-09-24 | The Procter & Gamble Company | Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis |
US6475765B1 (en) * | 1995-03-09 | 2002-11-05 | Procter & Gamble Company | Subtilisin DY variants having decreased adsorption and increased hydrolysis |
IL117350A0 (en) * | 1995-03-09 | 1996-07-23 | Procter & Gamble | Proteinase k variants having decreased adsorption and increased hydrolysis |
US6682924B1 (en) * | 1995-05-05 | 2004-01-27 | Novozymes A/S | Protease variants and compositions |
DE19530816A1 (en) * | 1995-08-23 | 1997-02-27 | Cognis Bio Umwelt | Use of mutant subtilisin protease in cosmetic products |
DE19535082A1 (en) | 1995-09-21 | 1997-03-27 | Henkel Ecolab Gmbh & Co Ohg | Paste-like detergent and cleaning agent |
DE19615776A1 (en) | 1996-04-20 | 1997-10-23 | Henkel Kgaa | Solubility-enhanced enzyme granules |
DE19636035A1 (en) | 1996-09-05 | 1998-03-12 | Henkel Ecolab Gmbh & Co Ohg | Paste-like detergent and cleaning agent |
KR100561826B1 (en) | 1996-11-04 | 2006-03-16 | 노보자임스 에이/에스 | Subtilase variants and compositions |
AU4773197A (en) | 1996-11-04 | 1998-05-29 | Novo Nordisk A/S | Subtilase variants and compositions |
EP0869167B2 (en) | 1996-12-09 | 2009-10-21 | Novozymes A/S | Reduction of phosphorus containing components in edible oils comprising a high amount of non-hydratable phosphorus by use of a phospholipase, a phospholipase from a filamentous fungus having phospholipase A and/or B activity |
DE19703364A1 (en) | 1997-01-30 | 1998-08-06 | Henkel Ecolab Gmbh & Co Ohg | Paste-like detergent and cleaning agent |
US6284246B1 (en) | 1997-07-30 | 2001-09-04 | The Procter & Gamble Co. | Modified polypeptides with high activity and reduced allergenicity |
AU9061898A (en) * | 1997-08-29 | 1999-03-22 | Novo Nordisk A/S | Protease variants and compositions |
KR20010023468A (en) | 1997-08-29 | 2001-03-26 | 한센 핀 베네드, 안네 제헤르, 웨이콥 마리안느 | Protease variants and compositions |
ATE385254T1 (en) * | 1997-08-29 | 2008-02-15 | Novozymes As | PROTEASE VARIANTS AND COMPOSITIONS |
ES2367505T3 (en) | 1997-10-23 | 2011-11-04 | Danisco Us Inc. | PROTEASE VARIANTS WITH MULTIPLE SUBSTITUTIONS WITH ALTERED NET LOAD FOR USE IN DETERGENTS. |
AR016969A1 (en) | 1997-10-23 | 2001-08-01 | Procter & Gamble | PROTEASE VARIANTE, ADN, EXPRESSION VECTOR, GUEST MICROORGANISM, CLEANING COMPOSITION, ANIMAL FOOD AND COMPOSITION TO TREAT A TEXTILE |
EP1032655B1 (en) | 1997-11-21 | 2005-06-29 | Novozymes A/S | Protease variants and compositions |
US6773907B2 (en) | 1997-11-21 | 2004-08-10 | Peter Kamp Hansen | Subtilase enzymes |
US6780629B2 (en) | 1997-11-21 | 2004-08-24 | Novozymes A/S | Subtilase enzymes |
CN1319002A (en) * | 1998-09-22 | 2001-10-24 | 宝洁公司 | Personal care compositions contaiing subtilisim enzymes bound to water insoluble substrates |
DE19857687A1 (en) | 1998-12-15 | 2000-06-21 | Henkel Ecolab Gmbh & Co Ohg | Pasty detergent |
CA2355655C (en) * | 1998-12-18 | 2011-03-15 | Novozymes A/S | Subtilase enzymes of the i-s1 and i-s2 sub-groups having an additional amino acid residue in an active site loop region |
WO2000037624A1 (en) * | 1998-12-18 | 2000-06-29 | Novozymes A/S | Subtilase enzymes of the i-s1 and i-s2 sub-groups having an additional amino acid residue in an active site loop region |
ATE371025T1 (en) * | 1998-12-18 | 2007-09-15 | Novozymes As | SUBTILASE ENZYMES OF THE I-S1 AND I-S2 SUBGROUPS WITH AN ADDITIONAL AMINO ACID RESIDUE IN AN ACTIVE LOOP REGION |
CN101275128B (en) * | 1998-12-18 | 2012-11-07 | 诺沃奇梅兹有限公司 | Subtilase enzymes of the I-S1 and I-S2 sub-groups having an additional amino acid residue in an active site loop region |
ATE342966T1 (en) * | 1998-12-18 | 2006-11-15 | Novozymes As | SUBTILASE ENZYMES OF THE I-S1 AND I-S2 SUBGROUPS, WITH AN ADDITIONAL AMINO ACID RESIDUE IN AN ACTIVE LOOP REGION |
DE69933694T2 (en) * | 1998-12-18 | 2007-08-23 | Novozymes A/S | SUBTILASE ENZYMES OF I-S1 and IS2 SUB-GROUPS, WITH AN ADDITIONAL AMINOSAUR EREST IN AN ACTIVE LOOP REGION |
ATE504651T1 (en) | 1998-12-18 | 2011-04-15 | Novozymes As | SUBTILASE ENZYMES OF THE I-S1 AND I-S2 SUBGROUPS WITH AN ADDITIONAL AMINO ACID RESIDUE IN AN ACTIVE LOOP REGION |
DE69937160T2 (en) * | 1998-12-18 | 2008-06-19 | Novozymes A/S | SUBTILASE ENZYMES OF I-S1 AND I-S2 SUB-GROUPS, WITH AN ADDITIONAL AMINO ACID RESISTANCE IN AN ACTIVE LOOP REGION |
DE69935349T2 (en) * | 1998-12-18 | 2007-11-15 | Novozymes A/S | SUBTILASES OF I-S1 AND I-S2 SUB-GROUPS WITH AN ADDITIONAL AMINO ACID RESISTANCE IN THE ACTIVE SIDE GRINDING REGION |
WO2000071691A1 (en) | 1999-05-20 | 2000-11-30 | Novozymes A/S | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 125 and 126 |
DE60040287D1 (en) | 1999-05-20 | 2008-10-30 | Novozymes As | SUBTILASE ENZYMS OF I-S1 AND I-S2 SUB-GROUPS WITH AT LEAST ONE ADDITIONAL AMINO ACID BETWEEN POSITIONS 128 AND 129 |
WO2000071689A1 (en) | 1999-05-20 | 2000-11-30 | Novozymes A/S | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 127 and 128 |
ATE408676T1 (en) | 1999-05-20 | 2008-10-15 | Novozymes As | SUBTILASE ENZYMES OF THE I-S1 AND I-S2 SUBGROUPS WITH AT LEAST ONE ADDITIONAL AMINO ACID BETWEEN POSITIONS 132 AND 133 |
EP1183338B2 (en) * | 1999-05-20 | 2012-06-13 | Novozymes A/S | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 97 and 98 |
AU4392400A (en) * | 1999-05-20 | 2000-12-12 | Novozymes A/S | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 131 and 132 |
DE60040284D1 (en) | 1999-05-20 | 2008-10-30 | Novozymes As | SUBTILASE ENZYMES OF I-S1 AND I-S2 SUB-GROUPS WITH AT LEAST ONE ADDITIONAL AMINO ACID BETWEEN POSITIONS 129 AND 130 |
EP1183340B1 (en) | 1999-05-20 | 2008-09-03 | Novozymes A/S | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 126 and 127 |
EP1214426A2 (en) | 1999-08-31 | 2002-06-19 | Novozymes A/S | Novel proteases and variants thereof |
US6727085B2 (en) | 1999-12-15 | 2004-04-27 | Fanoe Tina Sejersgaard | Subtilase variants having an improved wash performance on egg stains |
US6777218B1 (en) * | 2000-03-14 | 2004-08-17 | Novozymes A/S | Subtilase enzymes having an improved wash performance on egg stains |
CA2419896C (en) | 2000-08-21 | 2014-12-09 | Novozymes A/S | Subtilase enzymes |
CN100497617C (en) | 2000-10-13 | 2009-06-10 | 诺维信公司 | Subtilase variants |
DE10121463A1 (en) * | 2001-05-02 | 2003-02-27 | Henkel Kgaa | New alkaline protease variants and washing and cleaning agents containing these new alkaline protease variants |
DK200101090A (en) * | 2001-07-12 | 2001-08-16 | Novozymes As | Subtilase variants |
US7153820B2 (en) | 2001-08-13 | 2006-12-26 | Ecolab Inc. | Solid detergent composition and method for solidifying a detergent composition |
DE10153792A1 (en) * | 2001-10-31 | 2003-05-22 | Henkel Kgaa | New alkaline protease variants and washing and cleaning agents containing these new alkaline protease variants |
DE10162728A1 (en) | 2001-12-20 | 2003-07-10 | Henkel Kgaa | New alkaline protease from Bacillus gibsonii (DSM 14393) and washing and cleaning agents containing this new alkaline protease |
DE10163884A1 (en) | 2001-12-22 | 2003-07-10 | Henkel Kgaa | New alkaline protease from Bacillus sp. (DSM 14392) and detergents and cleaning agents containing this new alkaline protease |
EP1530631B1 (en) * | 2002-01-16 | 2013-08-07 | Genencor International, Inc. | Multiply-substituted protease variants |
AU2003210552A1 (en) * | 2002-01-16 | 2003-09-02 | Genencor International, Inc. | Multiply-substituted protease variants |
TWI319007B (en) | 2002-11-06 | 2010-01-01 | Novozymes As | Subtilase variants |
US7888093B2 (en) * | 2002-11-06 | 2011-02-15 | Novozymes A/S | Subtilase variants |
DE10260903A1 (en) * | 2002-12-20 | 2004-07-08 | Henkel Kgaa | New perhydrolases |
US7294499B2 (en) * | 2003-01-30 | 2007-11-13 | Novozymes A/S | Subtilases |
CA2526341C (en) | 2003-05-07 | 2013-02-19 | Novozymes A/S | Variant subtilisin enzymes (subtilases) |
JP4880469B2 (en) | 2003-10-23 | 2012-02-22 | ノボザイムス アクティーゼルスカブ | Protease with improved stability in detergents |
EP1735433B1 (en) * | 2004-04-02 | 2010-12-08 | Novozymes A/S | Subtilase variants having altered immunogenicity |
EP1700907A1 (en) | 2005-03-11 | 2006-09-13 | Unilever N.V. | Liquid bleaching composition |
EP1700904A1 (en) | 2005-03-11 | 2006-09-13 | Unilever N.V. | Liquid detergent composition |
US20070161531A1 (en) * | 2005-07-08 | 2007-07-12 | Novozymes A/S | Subtilase variants |
US20090215663A1 (en) | 2006-04-20 | 2009-08-27 | Novozymes A/S | Savinase variants having an improved wash performance on egg stains |
US8093200B2 (en) | 2007-02-15 | 2012-01-10 | Ecolab Usa Inc. | Fast dissolving solid detergent |
US20100190682A1 (en) | 2007-04-30 | 2010-07-29 | Sang-Kyu Lee | Use of protein hydrolysates to stabilize metalloprotease detergent formulations |
DE102007044415A1 (en) | 2007-09-17 | 2009-03-19 | Henkel Ag & Co. Kgaa | Performance-enhanced proteases and detergents and cleaners containing these proteases |
WO2009050684A2 (en) | 2007-10-18 | 2009-04-23 | Ecolab Inc. | Pressed, waxy, solid cleaning compositions and methods of making them |
EP2100947A1 (en) | 2008-03-14 | 2009-09-16 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
BR122013014157A2 (en) * | 2008-11-11 | 2018-11-13 | Danisco Us Inc. | cleaning compositions comprising bacillus subtilisin variants as well as cleaning process |
CN102209777B (en) * | 2008-11-11 | 2014-02-12 | 丹尼斯科美国公司 | Compositions and methods comprising subtilisin variant |
BR122013014156A2 (en) * | 2008-11-11 | 2015-07-14 | Danisco Us Inc | Cleaning composition comprising subtilisin variants as well as cleaning process |
US20100305019A1 (en) * | 2009-06-01 | 2010-12-02 | Lapinig Daniel Victoria | Hand Fabric Laundering System |
MX2012003387A (en) | 2009-09-25 | 2012-04-10 | Novozymes As | Use of protease variants. |
AU2010299799B2 (en) | 2009-09-25 | 2015-10-29 | Novozymes A/S | Subtilase variants |
US9045514B2 (en) | 2010-01-22 | 2015-06-02 | Dupont Nutrition Biosciences Aps | Methods for producing amino-substituted glycolipid compounds |
JP6018044B2 (en) * | 2010-04-15 | 2016-11-02 | ダニスコ・ユーエス・インク | Compositions and methods comprising protease variants |
DE102010028951A1 (en) | 2010-05-12 | 2011-11-17 | Henkel Ag & Co. Kgaa | Storage-stable liquid washing or cleaning agent containing protease and lipase |
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- 1995-04-18 MX MX9605358A patent/MX9605358A/en unknown
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- 1995-04-18 CZ CZ963217A patent/CZ321796A3/en unknown
- 1995-04-18 WO PCT/US1995/004760 patent/WO1995030011A2/en not_active Application Discontinuation
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WO1995030011A2 (en) | 1995-11-09 |
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JPH09512434A (en) | 1997-12-16 |
AU2294495A (en) | 1995-11-29 |
CN1151187A (en) | 1997-06-04 |
WO1995030011A3 (en) | 1995-12-14 |
CA2189427C (en) | 2003-10-28 |
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