CA2213854A1 - Multi-array, multi-specific electrochemiluminescence testing - Google Patents
Multi-array, multi-specific electrochemiluminescence testingInfo
- Publication number
- CA2213854A1 CA2213854A1 CA002213854A CA2213854A CA2213854A1 CA 2213854 A1 CA2213854 A1 CA 2213854A1 CA 002213854 A CA002213854 A CA 002213854A CA 2213854 A CA2213854 A CA 2213854A CA 2213854 A1 CA2213854 A1 CA 2213854A1
- Authority
- CA
- Canada
- Prior art keywords
- binding
- electrochemiluminescence
- binding domains
- electrode
- domains
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/66—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light electrically excited, e.g. electroluminescence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/806—Electrical property or magnetic property
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S977/00—Nanotechnology
- Y10S977/902—Specified use of nanostructure
- Y10S977/932—Specified use of nanostructure for electronic or optoelectronic application
- Y10S977/953—Detector using nanostructure
- Y10S977/957—Of chemical property or presence
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Plasma & Fusion (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Sustainable Development (AREA)
- Biophysics (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Abstract
Materials and methods are provided for producing patterned multi-array, multi-specific surfaces which are electronically excited for use in electromiluminescence based tests. Materials and methods are provided for the chemical and/or physical control of conducting domains and reagent deposition for use in flat panel displays and multiply specific testing procedures.
Claims (204)
1. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising an electrode and a counterelectrode, said electrode or counterelectrode having immobilized on a surface thereof a predetermined amount of a reagent capable of binding an electrochemiluminescent labelled species or a binding partner of an electrochemiluminescent labelled species.
2. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising an electrode and a counterelectrode, said electrode or counterelectrode having immobilized on a surface thereof a predetermined amount of a reagent capable of directly or indirectly binding an electrochemiluminescent labelled species.
3. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising an electrode, said electrode having immobilized on a surface thereof a predetermined amount of a reagent capable of binding a component of a binding electrochemiluminescence assay.
4. An apparatus as recited in claim 3 wherein said electrode is the working electrode.
5. An apparatus as recited in claim 3 wherein said electrode is the counterelectrode.
6. An apparatus as recited in claim 3 wherein the immobilized reagent and electrode complex is substantially permissive to electron transfer between said electrode and an electrochemiluminescent label.
7. An apparatus as recited in claim 3 wherein said electrode is comprised of a porous material.
8. An apparatus for detection of an analyte by electrochemiluminescence comprising a porous electrode and a counterelectrode.
9. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising a working electrode and a counterelectrode, said working electrode having immobilized on a surface thereof and in electrochemical contact therewith, a predetermined amount of a reagent capable of binding a component of a binding electrochemiluminescence assay.
10. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising a working electrode and a counterelectrode, said counterelectrode having immobilized on a surface thereof and in electrochemical contact therewith, a predetermined amount of a reagent capable of binding a component of a binding electrochemiluminescence assay.
11. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising an electrode, a counterelectrode and a porous matrix in electrochemical contact with said electrode, said porous matrix containing on a surface thereof a predetermined amount of a reagent capable of binding a component of a binding electrochemiluminescence assay.
12. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising an electrode and a plurality of discrete binding domains, said binding domains being capable of binding a component of a binding electrochemiluminescence assay.
13. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising a working electrode and a plurality of discrete binding domains each containing a predetermined amount of a reagent capable of binding a component of a binding electrochemiluminescence assay
14. An apparatus as recited in claim 13 further comprising a counterelectrode.
15. An apparatus as recited in claim 13 further including a counter electrode, sample delivery means and light detection means.
16. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising (a) an electrode, (b) a plurality of discrete binding domains each containing a predetermined amount of a reagent capable of binding a component of a binding electrochemiluminescence assay, (c) a counterelectrode, (d) means for sample delivery to said binding domains, (e) means for triggering electrochemiluminescence, and (f) means for electrochemiluminescence detection
17. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising (a) an electrode, (b) a support having immobilized thereon a plurality of discrete binding domains each containing a predetermined amount of a reagent capable of binding a component of a binding electrochemiluminescence assay, (c) a counterelectrode, (d) means for sample delivery to said binding domains, (e) means for triggering electrochemiluminescence, and (f) means for electrochemiluminescence detection
18. An apparatus as recited in claim 17 wherein said plurality of domains are in electrochemical contact with said electrode.
19. An apparatus as recited in claim 17 wherein said electrode is porous.
20. An apparatus as recited in claim 19 wherein said electrode is comprised of a carbonaceous material.
21. An apparatus as recited in claim 17 wherein said electrode is comprised of fibrils.
22. An apparatus as recited in claim 17 wherein said electrochemiluminescence detection means detects electrochemiluminescence from one or more of said discrete binding domains.
23. An apparatus as recited in claim 17 wherein said support is a porous matrix.
24. An apparatus as recited in claim 17 wherein said support is a porous matrix and said electrode is porous.
25. An apparatus as recited in claim 17 wherein said support is a porous matrix, said matrix being positioned between said electrode and counterelectrode.
26. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising (a) an electrode, (b) a plurality of discrete binding domains each containing a predetermined amount of a reagent capable of binding a component of a binding electrochemiluminescence assay, (c) a counterelectrode, (d) means for sample delivery to said binding domains, (e) means for triggering electrochemiluminescence, and (f) a plurality of means for electrochemiluminescence detection.
27. An apparatus as recited in claim 26 wherein said plurality of means for electrochemiluminescence detection is used to image one or more of said binding domains.
28. An apparatus as recited in claim 27 wherein at least one of said plurality of detection means detects electrochemiluminescence from one binding domain.
29. An apparatus as recited in claim 27 wherein said plurality of means for electrochemiluminescence detection is a CCD array or a diode array.
30. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising an electrode having immobilized on a surface thereof a plurality of discrete binding domains, said binding domains being capable of binding a component of a binding electrochemiluminescence assay.
31. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising an electrode having immobilized on a surface thereof a plurality of discrete binding domains each containing a predetermined amount of a reagent capable of binding a component of a binding electrochemiluminescence assay.
32. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising an electrode having immobilized on a surface thereof a plurality of discrete binding domains, said binding domains containing an electrochemiluminescent label.
33. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising an electrode having immobilized on a surface thereof a plurality of discrete binding domains, said binding domains being capable of generating a plurality of electrochemiluminescence signals; and a counterelectrode.
34. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising a support having immobilized thereon a plurality of discrete domains each containing a predetermined amount of a reagent capable of binding a component of a binding electrochemiluminescence assay.
35. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising an electrode and a support having immobilized on a surface thereof a plurality of discrete binding domains each containing a predetermined amount of a reagent capable of binding a component of a binding electrochemiluminescence assay, said electrode and said binding domains being in electrochemical contact.
36. An apparatus as recited in claim 35 wherein the electrode is the working electrode.
37. An apparatus as recited in claim 35 wherein the electrode is the counterelectrode.
38. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising:
(a) an electrochemical cell (b) a first surface containing an electrode (c) a second surface having immobilized thereon a plurality of binding domains each containing a binding reagent capable of binding a component of a binding electrochemiluminescence assay, said first and second surfaces being spatially aligned to permit electron transfer between said electrode and an electrochemiluminescence label directly or indirectly linked to a said binding reagent.
(a) an electrochemical cell (b) a first surface containing an electrode (c) a second surface having immobilized thereon a plurality of binding domains each containing a binding reagent capable of binding a component of a binding electrochemiluminescence assay, said first and second surfaces being spatially aligned to permit electron transfer between said electrode and an electrochemiluminescence label directly or indirectly linked to a said binding reagent.
39. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising an electrochemical cell and an electrode having immobilized thereon a plurality of binding domains each containing a binding reagent capable of binding a component of a binding electrochemiluminescence assay.
40. A method for conducting a plurality of electrochemiluminescence assays comprising the steps of:
(a) contacting a plurality of discrete binding domains with a sample containing one or a plurality of analytes of interest under assay conditions;
(b) causing one or more of said plurality of domains to electrochemiluminescence; and (c) detecting electrochemiluminescence at a plurality of said binding domains.
(a) contacting a plurality of discrete binding domains with a sample containing one or a plurality of analytes of interest under assay conditions;
(b) causing one or more of said plurality of domains to electrochemiluminescence; and (c) detecting electrochemiluminescence at a plurality of said binding domains.
41. A method for conducting a plurality of electrochemiluminescence assays for a plurality of different analytes of interest comprising the steps of:
(a) contacting a plurality of discrete binding domains, with a sample containing a plurality of analytes of interest under assay conditions;
(b) causing said plurality of discrete binding domains to simultaneously electrochemiluminescence; and (c) simultaneously detecting electrochemiluminescence from each of said discrete binding domains.
(a) contacting a plurality of discrete binding domains, with a sample containing a plurality of analytes of interest under assay conditions;
(b) causing said plurality of discrete binding domains to simultaneously electrochemiluminescence; and (c) simultaneously detecting electrochemiluminescence from each of said discrete binding domains.
42. An article comprising a plurality of discrete binding domains on a support, said binding domains having a relative spatial organization with respect to one another and having different binding specificities for binding a plurality of different analytes of interest in an electrochemiluminescence assay.
43. An article comprising a plurality of discrete binding domains on a support each of said domains having a relative spatial organization with respect to one another and each having a different binding specificity for an analyte of interest in an electrochemiluminescence assay, each domain containing a different binding component bound to a different analyte of interest.
44. An article comprising a plurality of discrete binding domains on a support, said domains having a relative spatial organization with respect to one another and each having a different binding specificity for an analyte of interest in an electrochemiluminescence assay, each domain being bound directly or indirectly, to a different analyte of interest and to a moiety capable of electrochemiluminescence.
45. An article comprising a plurality of discrete binding domains on a support comprising a porous material each of said domains having a relative spatial organization with respect to one another and each having a different binding specificity for an analyte of interest in an electrochemiluminescence assay each domain containing a different binding component bound to a different analyte of interest.
46. An article as recited in claim 45, wherein said porous material comprises carbon.
47. An article as recited in claim 45 which said porous material comprises fibrils.
48. An article comprising a plurality of discrete binding domains on a support comprising a functionalized fibril, said domains having a relative spatial organization with respect to one another and each having a different binding specificity for an analyte of interest in an electrochemiluminescence assay, each domain being bound directly or indirectly, to a different analyte of interest and to a moiety capable of electrochemiluminescence.
49. An article comprising a plurality of binding domains on a support comprising one or more polymer matrices, said domains having a relative spatial organization with respect to one another and each having a different binding specificity for an analyte of interest in an electrochemiluminescence assay, each domain being bound directly or indirectly, to a different analyte of interest and to a moiety capable of electrochemiluminescence.
50. A cassette for use in the detection of an analyte in a sample by electrochemiluminescence comprising:
(a) a plurality of discrete binding domains on a support; and (b) one or more pairs of electrodes and counterelectrodes.
(a) a plurality of discrete binding domains on a support; and (b) one or more pairs of electrodes and counterelectrodes.
51. A cassette for using the detection of an analyte in a sample by electrochemiluminescence comprising:
(a) a plurality of discrete binding domains on a support;
(b) one or more pairs of electrodes and counterelectrodes spatially aligned with said discrete binding domains; and (c) means for sample delivery onto said plurality of discrete binding domains.
(a) a plurality of discrete binding domains on a support;
(b) one or more pairs of electrodes and counterelectrodes spatially aligned with said discrete binding domains; and (c) means for sample delivery onto said plurality of discrete binding domains.
52. A cassette as recited in claim 49 wherein said plurality of discrete binding domains forms at least one surface capable of binding a component of a binding electrochemiluminescence assay.
53. A cassette as recited in claim 50 wherein said plurality of binding domains includes binding domains having different binding specificities to provide for simultaneous binding of a plurality of different analytes of interest present in a sample.
54. A cassette for detecting or measuring electrochemiluminescence comprising:
(a) a first support having a plurality of discrete binding domains thereon;
(b) a plurality of electrode and counterelectrode pairs, each of said plurality of discrete binding domains being aligned with an in proximity to one of said plurality of electrode and counterelectrode pairs, said discrete binding domains and electrode and counterelectrode pairs forming a plurality of cells for detecting or conducting measurements of electrochemiluminescence, said electrode/counterelectrode pairs being addressable by a source of electrical energy in the form of a voltage waveform effective to trigger electrochemiluminescence; and (c) means for sample delivery onto said plurality of discrete binding domains.
(a) a first support having a plurality of discrete binding domains thereon;
(b) a plurality of electrode and counterelectrode pairs, each of said plurality of discrete binding domains being aligned with an in proximity to one of said plurality of electrode and counterelectrode pairs, said discrete binding domains and electrode and counterelectrode pairs forming a plurality of cells for detecting or conducting measurements of electrochemiluminescence, said electrode/counterelectrode pairs being addressable by a source of electrical energy in the form of a voltage waveform effective to trigger electrochemiluminescence; and (c) means for sample delivery onto said plurality of discrete binding domains.
55. A cassette as recited in claim 53 wherein said plurality of binding domains includes binding domains having different binding specificities to provide for simultaneous binding of a plurality of different analytes of interest present in a sample.
56. A cassette for detecting or measuring an analyte of interest in a sample comprising:
(a) a first support having a plurality of discrete binding domains on a surface thereof, at least one of said discrete binding domains being of different binding specificity than the other binding domains, each of said plurality of discrete binding domains being hydrophilic and surrounded by hydrophobic regions;
(b) a second support having a plurality of hydrophilic domains comprising reaction media suitable for conducting a chemical assay thereon; and (c) means for bringing said plurality of discrete binding domains and said plurality of reaction media into contact so that a sample to be analyzed present on each binding domain is contacted with a reaction medium.
(a) a first support having a plurality of discrete binding domains on a surface thereof, at least one of said discrete binding domains being of different binding specificity than the other binding domains, each of said plurality of discrete binding domains being hydrophilic and surrounded by hydrophobic regions;
(b) a second support having a plurality of hydrophilic domains comprising reaction media suitable for conducting a chemical assay thereon; and (c) means for bringing said plurality of discrete binding domains and said plurality of reaction media into contact so that a sample to be analyzed present on each binding domain is contacted with a reaction medium.
57. A cassette as recited in claim 50 in which said discrete binding domains further comprise an internal control.
58. A method for preparing a plurality of discrete binding domains on a support containing binding reagents capable of binding to analytes of interest, comprising the steps of (a) forming a self assembled monolayer on a support, said monolayer including a linking group A on the monolayer surface not adjacent to the support, said first linking group A being capable of specifically binding to a linking group B; and (b) contacting said first linking group A
with a binding reagent capable of binding to an analyte of interest, said binding reagent being bound to said linking group B, so that said binding reagent is linked to said monolayer by an A:B linkage to form a binding surface, said binding surface organized as a plurality of discrete binding domains.
with a binding reagent capable of binding to an analyte of interest, said binding reagent being bound to said linking group B, so that said binding reagent is linked to said monolayer by an A:B linkage to form a binding surface, said binding surface organized as a plurality of discrete binding domains.
59. A process according to claim 57 wherein a plurality of different binding reagents are bound to the plurality of discrete binding domains and wherein said contacting step is carried out by delivering a plurality of fluid samples, each fluid sample including a different binding reagent, onto said monolayer from a plurality of fluidic guides.
60. A method for detecting or measuring an analyte in an electrochemiluminescence binding assay comprising the steps of:
(a) contacting a plurality of discrete binding domains immobilized on a surface of one or more supports with a sample containing a plurality of analytes and a component of said assay linked to an electrochemiluminescence label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at one or more of said domains in the presence of a reaction medium suitable for conducting an electrochemiluminescence assay; and (c) detecting or measuring electrochemiluminescence from said plurality of domains.
(a) contacting a plurality of discrete binding domains immobilized on a surface of one or more supports with a sample containing a plurality of analytes and a component of said assay linked to an electrochemiluminescence label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at one or more of said domains in the presence of a reaction medium suitable for conducting an electrochemiluminescence assay; and (c) detecting or measuring electrochemiluminescence from said plurality of domains.
61. A kit for use in performance, of a plurality of electrochemiluminescence assays for a plurality of analytes of interest comprising:
(a) an article comprising a plurality of discrete binding domains on a support, said binding domains having a relative spatial organization with respect to one another and having different binding specificities for a plurality of different analytes of interest in an electrochemiluminescence assay; and (b) a vessel containing a reagent necessary for the conduct of said assays.
(a) an article comprising a plurality of discrete binding domains on a support, said binding domains having a relative spatial organization with respect to one another and having different binding specificities for a plurality of different analytes of interest in an electrochemiluminescence assay; and (b) a vessel containing a reagent necessary for the conduct of said assays.
62. A kit as recited in claim 60 further including an apparatus for performing said assays.
63. A kit for use in performance, of a plurality of electrochemiluminescence assays for a plurality of analytes of interest comprising:
a vessel containing binding components specific for a plurality of different analytes for use in a plurality of electrochemiluminescence assays.
a vessel containing binding components specific for a plurality of different analytes for use in a plurality of electrochemiluminescence assays.
64. A kit as recited in claim 62 further containing one or more vessels containing non-binding components for a plurality of electrochemiluminescence assays.
65. A kit as recited in claim 62 further containing an article comprising a plurality of discrete binding domains on a support, said binding domains having a relative spatial organization with respect to one another and having different binding specificities for a plurality of different analytes of interest in an electrochemiluminescence assay.
66. A cassette comprising:
(a) a plurality of discrete binding domains on a support, forming at least one binding surface;
(b) a plurality of electrode and counterelectrode pairs, in which said discrete binding domains are spatially aligned with said plurality of electrode and counterelectrode pairs; and (c) means for sample delivery onto said plurality of discrete binding domains.
(a) a plurality of discrete binding domains on a support, forming at least one binding surface;
(b) a plurality of electrode and counterelectrode pairs, in which said discrete binding domains are spatially aligned with said plurality of electrode and counterelectrode pairs; and (c) means for sample delivery onto said plurality of discrete binding domains.
67. A cassette comprising:
(a) a plurality of discrete binding domains on a support, forming at least one binding surface; and (b) a plurality of electrode and counterelectrode pairs, in which said discrete binding domains are spatially aligned with and capable of being brought into proximity to said plurality of electrode and counterelectrode pairs, wherein said binding domains are hydrophilic or hydrophobic relative to the surface of the support.
(a) a plurality of discrete binding domains on a support, forming at least one binding surface; and (b) a plurality of electrode and counterelectrode pairs, in which said discrete binding domains are spatially aligned with and capable of being brought into proximity to said plurality of electrode and counterelectrode pairs, wherein said binding domains are hydrophilic or hydrophobic relative to the surface of the support.
68. A cassette comprising:
(a) a plurality of discrete binding domains on a first support, forming at least one binding surface;
(b) a plurality of electrode and counterelectrode pairs in which said discrete binding domains are spatially aligned with said plurality of electrode and counterelectrode pairs, the plurality of electrodes is on said binding surface, each electrode adjacent to a binding domain, and the counterelectrodes are on a second support; and (c) a means for sample delivery onto said plurality of discrete binding domains.
(a) a plurality of discrete binding domains on a first support, forming at least one binding surface;
(b) a plurality of electrode and counterelectrode pairs in which said discrete binding domains are spatially aligned with said plurality of electrode and counterelectrode pairs, the plurality of electrodes is on said binding surface, each electrode adjacent to a binding domain, and the counterelectrodes are on a second support; and (c) a means for sample delivery onto said plurality of discrete binding domains.
69. The cassette according to claim 68 in which said binding domains are hydrophilic or hydrophobic relative to the surface of the support.
70. A cassette according to claim 66 wherein said support contains from 2 through 500 binding domains.
71. A cassette according to claim 66 wherein said plurality of binding domains comprises binding domains having differing binding specificities to provide for simultaneous binding of multiple different analytes of interest present in a sample.
72. A cassette for detecting or measuring electrochemiluminescence comprising:
(a) a first support having a plurality of discrete binding domains thereon;
(b) a plurality of electrode and counterelectrode pairs, said plurality of discrete binding domains being aligned with and in proximity to said plurality of electrode and counterelectrode pairs, said discrete binding domains and electrode and counterelectrode pairs forming a plurality of cells for detecting or conducting measurements of electrochemiluminescence, said electrode/counterelectrode pairs being addressable by a source of electrical energy in the form of a voltage waveform effective to trigger electrochemiluminescence; and (c) a means for sample delivery onto said plurality of discrete binding domains.
(a) a first support having a plurality of discrete binding domains thereon;
(b) a plurality of electrode and counterelectrode pairs, said plurality of discrete binding domains being aligned with and in proximity to said plurality of electrode and counterelectrode pairs, said discrete binding domains and electrode and counterelectrode pairs forming a plurality of cells for detecting or conducting measurements of electrochemiluminescence, said electrode/counterelectrode pairs being addressable by a source of electrical energy in the form of a voltage waveform effective to trigger electrochemiluminescence; and (c) a means for sample delivery onto said plurality of discrete binding domains.
73. The cassette according to claim 72 which further comprises a second support capable of being placed adjacent to said first support to provide sample containing means therebetween, wherein said plurality of electrode and counterelectrode pairs are affixed to said second support.
74. The cassette according to claim 72 wherein said plurality of electrode and counterelectrode pairs are affixed to said first support.
75. The cassette according to claim 72 which further comprises a second support capable of being placed adjacent to said first support to provide sample containing means therebetween, wherein a plurality of electrodes is positioned on said first support and a plurality of counterelectrodes is positioned on said second support, so that said plurality of electrodes and counterelectrodes is capable of being positioned in proximity to one another.
76. The cassette according to claim 72 wherein said plurality of discrete binding domains contains from 5 through 1000 binding domains.
77. The cassette according to claim 72 wherein said plurality of discrete binding domains comprises at least one binding domain that contains binding reagents that are identical to each other and that differ in specificity from the binding reagents contained within other binding domains, to provide for binding of multiple different analytes of interest.
78. The cassette according to claim 72 wherein said plurality of discrete binding domains comprises at least one binding domain that contains binding reagents that differ in binding specificity.
79. The cassette according to claim 78 further comprising a reflective surface adjacent to said first or said second supports.
80. The cassette according to claim 78 wherein said first and second supports, the plurality of discrete binding domains and the plurality of electrode and counterelectrode pairs are substantially transparent.
81. The cassette according to claim 72 wherein said electrode and counterelectrode pairs are in a size range of from 0.001 to 10 mm in diameter or width.
82. The cassette according to claim 80 wherein said binding domains are in a size range of from 0.001 to 10 mm in diameter or width.
83. The cassette according to claim 72 wherein said electrode and counterelectrode pairs are each separately addressable by at least one source of electrical energy.
84. The cassette according to claim 72 wherein said binding domains are hydrophilic and are surrounded by a hydrophobic surface.
85. The cassette according to claim 72 wherein said binding domains are hydrophobic and are surrounded by a hydrophilic surface.
86. The cassette according to claim 72 wherein said binding surface comprises a patterned, self-assembled monolayer.
87. The cassette according to claim 86 wherein said patterned self-assembled monolayer comprises alkane thiols.
88. The cassette according to claim 87 wherein said binding domains are capable of binding to analytes selected from the group consisting of proteins, nucleic acids, carbohydrate moieties, antibodies, antigens, cells, organic compounds, and organometallic compounds.
89. The cassette according to claim 72 wherein said binding domains contain functional reagents in assays selected from the group consisting of clinical chemistry assays, chemiluminescence assays, immunoassays and nucleic acid probe assays.
90. The cassette according to claim 73 further comprising a removable electrode protection barrier interposed between said second support and said discrete binding domains, said electrode protection barrier being removable prior to triggering said electrochemiluminescence.
91. The cassette according to claim 72 further comprising an electrically conducting material on or adjacent to a plurality of electrodes of said electrode pairs, positioned so as to extend an electrical potential away from the electrode surface into an assay medium.
92. An apparatus for measuring electrochemiluminescence of a sample comprising:
(a) a plurality of cells for holding at least one sample, said plurality of cells being formed from a plurality of electrode and counterelectrode pairs and a first support that comprises a plurality of discrete binding domains, said plurality of discrete binding domains being aligned with and in proximity to the plurality of electrode and counterelectrode pairs, said electrode and counterelectrode pairs being separately addressable, said cells being suitable for conducting measurements of electrochemiluminescence, (b) voltage control means adapted to apply a controlled voltage waveform to said plurality of electrode and counterelectrode pairs, said voltage waveform being effective to trigger electrochemiluminescence in said plurality of cells, and (c) photon detector means for detecting electrochemiluminescence from said sample.
(a) a plurality of cells for holding at least one sample, said plurality of cells being formed from a plurality of electrode and counterelectrode pairs and a first support that comprises a plurality of discrete binding domains, said plurality of discrete binding domains being aligned with and in proximity to the plurality of electrode and counterelectrode pairs, said electrode and counterelectrode pairs being separately addressable, said cells being suitable for conducting measurements of electrochemiluminescence, (b) voltage control means adapted to apply a controlled voltage waveform to said plurality of electrode and counterelectrode pairs, said voltage waveform being effective to trigger electrochemiluminescence in said plurality of cells, and (c) photon detector means for detecting electrochemiluminescence from said sample.
93. A kit suitable for measuring electrochemiluminescence comprising in one or more containers (a) a plurality of discrete binding domains on a support, forming at least one binding surface; and (b) a plurality of electrode and counterelectrode pairs which are spatially aligned with and capable of being brought into proximity to said plurality of discrete binding domains.
94. The kit according to claim 93 further comprising at least one second support capable of being placed adjacent to said binding surface support to provide sample containing means.
95. The kit according to claim 94 wherein said second support bears said plurality of electrode and counterelectrode pairs capable of being aligned with and brought into proximity to said plurality of discrete binding domains.
96. The kit according to claim 93 which further comprises reagents suitable for conducting an electrochemiluminescent assay and a predetermined amount of a purified analyte of interest.
97. A cassette for detecting or measuring an analyte of interest in a sample comprising (a) a first support having a plurality of discrete binding domains on the surface thereof to form at least one binding surface, at least some of said discrete binding domains being of different binding specificities than other binding domains, each of said plurality of discrete binding domains being hydrophilic and surrounded by hydrophobic regions and (b) a second support having a plurality of hydrophilic domains comprising reaction media suitable for conducting a chemical assay thereon to form an assay surface, said plurality of discrete binding domains and said plurality of said reaction media being capable of being brought into contact so that a sample to be analyzed present on each binding domain is contacted with a reaction medium to detect or measure an analyte of interest.
98. The cassette according to claim 72 which further comprises temperature control means.
99. A cassette for performing a reaction of interest comprising:
(a) a first support having a plurality of discrete domains on the surface of the support, each of said domains being hydrophilic and surrounded by a hydrophobic region on said first support surface; and (b) a second support having a plurality of discrete domains on the surface of the second support, each of said domains (i) being hydrophilic and surrounded by a hydrophobic region on said second support surface, (ii) comprising reaction medium suitable for performing a reaction of interest, and (iii) being spatially aligned with the domains on said first support surface such that said second support can be situated so as to bring each of said domains on said second support surface into contact with an aligned domain on said first support surface.
(a) a first support having a plurality of discrete domains on the surface of the support, each of said domains being hydrophilic and surrounded by a hydrophobic region on said first support surface; and (b) a second support having a plurality of discrete domains on the surface of the second support, each of said domains (i) being hydrophilic and surrounded by a hydrophobic region on said second support surface, (ii) comprising reaction medium suitable for performing a reaction of interest, and (iii) being spatially aligned with the domains on said first support surface such that said second support can be situated so as to bring each of said domains on said second support surface into contact with an aligned domain on said first support surface.
100. The cassette according to claim 66 in which said discrete binding domains further comprise an internal control.
101. The apparatus according to claim 92 in which said plurality of discrete binding domains includes at least two identical binding domains.
102. A binding surface that is the product of the following process:
(a) forming a self assembled monolayer on a support, said monolayer comprising a first linking group A on the monolayer surface not adjacent to the support, said first linking group A capable of specifically binding to a second linking group B, said monolayer being applied to at least one domain on said support; and (b) contacting said first linking group A
with a binding reagent, said binding reagent capable of binding to an analyte of interest, said binding reagent being linked to said linking group B, so that said binding reagent is linked to said monolayer by an A:B
linkage to form a binding surface, said binding surface being organized as a plurality of discrete binding domains containing said binding reagents capable of binding to an analyte of interest.
(a) forming a self assembled monolayer on a support, said monolayer comprising a first linking group A on the monolayer surface not adjacent to the support, said first linking group A capable of specifically binding to a second linking group B, said monolayer being applied to at least one domain on said support; and (b) contacting said first linking group A
with a binding reagent, said binding reagent capable of binding to an analyte of interest, said binding reagent being linked to said linking group B, so that said binding reagent is linked to said monolayer by an A:B
linkage to form a binding surface, said binding surface being organized as a plurality of discrete binding domains containing said binding reagents capable of binding to an analyte of interest.
103. An apparatus comprising the binding surface of claim 102.
104. The cassette according to claim 66 in which said support comprises an elastomeric material.
105. A process for preparing a plurality of discrete binding domains on a support comprising the steps of (a) forming a self assembled monolayer on a support, said monolayer comprising a first linking group A on the monolayer surface not adjacent to the support, said first linking group A capable of specifically binding to a second linking group B, said monolayer being applied to at least one domain on said support; and (b) contacting said first linking group A
with a binding reagent, said binding reagent capable of binding to an analyte of interest, said binding reagent being linked to said linking group B, so that said binding reagent is linked to said monolayer by an A:B
linkage to form a binding surface, said binding surface being organized as a plurality of discrete binding domains containing said binding reagents capable of binding to an analyte of interest.
with a binding reagent, said binding reagent capable of binding to an analyte of interest, said binding reagent being linked to said linking group B, so that said binding reagent is linked to said monolayer by an A:B
linkage to form a binding surface, said binding surface being organized as a plurality of discrete binding domains containing said binding reagents capable of binding to an analyte of interest.
106. The process according to claim 105 wherein in step (a) said monolayer is produced as a pattern on said support by a method selected from the group consisting of micro-etching, micro-pen deposition and micro-stamping.
107. The process according to claim 105 wherein said binding reagent is selected from the group consisting of proteins and fragments and derivatives thereof, and nucleic acids and fragments and derivatives thereof.
108. The process according to claim 105 wherein said binding reagent is selected from the group consisting of antibodies and binding fragments thereof, antigens, epitopes, cells and cellular components, enzymes, enzyme substrates, lectins, protein A, protein G, organic compounds, organometallic compounds and carbohydrate moieties.
109. The process according to claim 105 wherein said binding reagent comprises a plurality of different binding reagents, and said contacting step is carried out by delivering a plurality of fluid samples, each fluid sample comprising a different binding reagent, onto said monolayer from a multi array of fluidic guides, such that discrete binding domains of the binding surface on said monolayer have different binding reagents linked thereto.
110. A method for detecting or measuring electrochemiluminescence comprising (a) contacting a plurality of discrete binding domains situated on a surface of one or more supports with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at each of a plurality of electrode and counterelectrode pairs spatially aligned with and in proximity to said plurality of discrete binding domains, and (c) detecting or measuring said electrochemiluminescence.
(b) applying a voltage waveform effective to trigger electrochemiluminescence at each of a plurality of electrode and counterelectrode pairs spatially aligned with and in proximity to said plurality of discrete binding domains, and (c) detecting or measuring said electrochemiluminescence.
111. The method according to claim 110 wherein said plurality of discrete binding domains is in a range of from 5 through 1000 binding domains.
112. The method according to claim 110 wherein said plurality of discrete binding domains comprises at least one binding domain that contains binding reagents that are identical to each other and that differ in specificity from the binding reagents contained within other binding domains, to provide for binding of multiple different analytes of interest.
113. The method according to claim 110 wherein said support comprises a reflective surface.
114. The method according to claim 110 wherein said support, the binding surface and the electrodes and counterelectrode pairs are substantially transparent.
115. The method according to claim 110 wherein said binding domains are in a size range of from 0.001 to 10 mm in diameter or width.
116. The method according to claim 110 wherein said electrode and counterelectrode pairs are separately addressable by at least one source of electrical energy.
117. The method according to claim 110 wherein said binding domains are hydrophilic and are surrounded by a hydrophobic surface.
118. The method according to claim 110 wherein said binding domains are hydrophobic and are surrounded by a hydrophilic surface.
119. The method according to claim 110 wherein said binding domains are patterned, self-assembled monolayers.
120. The method according to claim 119 wherein said binding domains are capable of specifically binding an analyte selected from the group consisting of proteins, nucleic acids, carbohydrate moieties, antibodies, antigens, cells and cellular components, organic compounds, and organometallic compounds.
121. The method according to claim 110 wherein said method further comprises contacting said binding domains with reaction medium suitable for conducting an assay selected from the group consisting of clinical chemistry assays, immunoassays and nucleic acid probe assays.
122. The method according to claim 110 wherein said one or more supports is a plurality of supports situated in a stack and at least a portion of said supports are substantially transparent to light generated by electrochemiluminescence, each of said plurality of supports being positioned adjacent to another of said supports.
123. A method for detecting or measuring electrochemiluminescence in a sample comprising (a) contacting one or more of a plurality of discrete binding domains, said plurality of binding domains situated on a surface of one or more supports, with a sample comprising molecules linked to an electrochemiluminescent label, wherein a plurality of electrode and counterelectrode pairs are spatially aligned with said plurality of discrete binding domains, wherein said plurality of electrode and counterelectrode pairs is present on a second support surface, (b) placing said second support surface proximate to said binding surface so that each said electrode and counterelectrode pair is proximate to a different binding domain, (c) applying a voltage waveform effective to trigger electrochemiluminescence at each of said plurality of electrode and counterelectrode pairs, and (d) detecting or measuring said electrochemiluminescence.
124. The method according to claim 110 wherein during step (a) said plurality of electrode and counterelectrode pairs are protected from the sample by a removable electrode protection barrier, and said method further comprises removing said barrier prior to applying said voltage waveform.
125. A method for detecting or measuring analytes of interest in a sample comprising (a) placing drops of a sample containing an analyte to be detected or measured on a plurality of discrete binding domains on a support surface, said plurality of discrete binding domains comprising at least one binding domain that contains binding reagents that are identical to each other and that differ in specificity from the binding reagents contained within other binding domains, each of said discrete binding domains being characterized as either hydrophobic or hydrophilic, with the proviso that the region of said support surface surrounding each said binding domain is (i) hydrophobic if said binding domain is hydrophilic, and (ii) hydrophilic if said binding domain is hydrophobic, so as to allow said one or more analytes of interest in the sample to bind to said binding domains, and (b) contacting said drops on said first support with a surface of a second support having a plurality of discrete hydrophilic domains comprising reaction media suitable for conducting a chemical assay thereon, and (c) determining the presence of said analytes of interest that are bound to said binding domain.
126. A method for detecting or measuring analytes of interest in a sample comprising (a) placing drops of a sample containing an analyte to be detected or measured on a plurality of discrete binding domains on a support surface, said plurality of discrete binding domains comprising at least one binding domain that contains binding reagents that are identical to each other and that differ in specificity from the binding reagents contained within other binding domains, each of said discrete binding domains being characterized as either hydrophobic or hydrophilic, with the proviso that the region of said support surface surrounding each said binding domain is (i) hydrophobic if said binding domain is hydrophilic, and (ii) hydrophilic if said binding domain is hydrophobic, so as to allow said one or more analytes of interest in the sample to bind to said binding domains, and (b) placing drops of a reaction medium on said drops of sample; and (c) determining the presence of said analytes of interest that are bound to said binding domain.
127. A method for detecting or measuring electrochemiluminescence in a sample comprising the following steps in the order stated:
(a) contacting a sample with a surface of a support, said surface containing a plurality of discrete binding domains, said binding domains being spatially aligned with and capable of being brought into proximity to a plurality of electrode and counterelectrode pairs;
(b) bringing said binding domains into proximity to said plurality of electrode and counterelectrode pairs;
(c) applying a voltage waveform effective to trigger electrochemiluminescence; and (d) detecting or measuring electrochemiluminescence.
(a) contacting a sample with a surface of a support, said surface containing a plurality of discrete binding domains, said binding domains being spatially aligned with and capable of being brought into proximity to a plurality of electrode and counterelectrode pairs;
(b) bringing said binding domains into proximity to said plurality of electrode and counterelectrode pairs;
(c) applying a voltage waveform effective to trigger electrochemiluminescence; and (d) detecting or measuring electrochemiluminescence.
128. A method for detecting or measuring electrochemiluminescence in a sample comprising:
(a) contacting a sample with a surface of a support, said surface containing a plurality of discrete binding domains;
(b) scanning an electrode and counterelectrode pair over the surface of said support in proximity to said binding domains, while applying a voltage waveform effective to trigger electrochemiluminescence; and (c) detecting or measuring electrochemiluminescence.
(a) contacting a sample with a surface of a support, said surface containing a plurality of discrete binding domains;
(b) scanning an electrode and counterelectrode pair over the surface of said support in proximity to said binding domains, while applying a voltage waveform effective to trigger electrochemiluminescence; and (c) detecting or measuring electrochemiluminescence.
129. The process according to claim 109 in which each said binding domain is prepared so that it is spatially aligned with and in proximity to a plurality of electrode and counterelectrode pairs.
130. A method of detecting or measuring an analyte of interest in a sample comprising:
(a) contacting a sample with a surface of a support, said surface containing a plurality of discrete binding domains, said binding domains being spatially aligned with and capable of being brought into proximity to a plurality of electrode and counterelectrode pairs, said binding domains (i) containing an electrochemiluminescent label, and (ii) capable of binding an analyte of interest, in which said contacting is under conditions such that binding of any analyte in the sample to said binding domains can occur;
(b) bringing said binding domains into proximity to said plurality of electrode and counterelectrode pairs;
(c) applying a voltage waveform effective to trigger electrochemiluminescence; and (d) detecting or measuring electrochemiluminescence, in which a decrease in electrochemiluminescence relative to the electrochemiluminescence observed when said contacting step is not carried out or is carried out in the absence of any analyte of interest in the sample, indicates the presence or amount of said analyte in the sample.
(a) contacting a sample with a surface of a support, said surface containing a plurality of discrete binding domains, said binding domains being spatially aligned with and capable of being brought into proximity to a plurality of electrode and counterelectrode pairs, said binding domains (i) containing an electrochemiluminescent label, and (ii) capable of binding an analyte of interest, in which said contacting is under conditions such that binding of any analyte in the sample to said binding domains can occur;
(b) bringing said binding domains into proximity to said plurality of electrode and counterelectrode pairs;
(c) applying a voltage waveform effective to trigger electrochemiluminescence; and (d) detecting or measuring electrochemiluminescence, in which a decrease in electrochemiluminescence relative to the electrochemiluminescence observed when said contacting step is not carried out or is carried out in the absence of any analyte of interest in the sample, indicates the presence or amount of said analyte in the sample.
131. A method of detecting or measuring an analyte of interest in a sample comprising:
(a) contacting a sample with a surface of a support, said surface containing a plurality of discrete binding domains, said binding domains being spatially aligned with and capable of being brought into proximity to a plurality of electrode and counterelectrode pairs, said binding domains being capable of binding an analyte of interest, in which said contacting is under conditions such that binding of any analyte in the sample to said binding domains can occur, and in which said sample contains molecules that are linked to an electrochemiluminescent label;
(b) bringing said binding domains into proximity to said plurality of electrode and counterelectrode pairs;
(c) applying a voltage waveform effective to trigger electrochemiluminescence; and (d) detecting or measuring electrochemiluminescence, in which an increase in electrochemiluminescence over background levels indicates the presence or amount of the analyte in the sample.
(a) contacting a sample with a surface of a support, said surface containing a plurality of discrete binding domains, said binding domains being spatially aligned with and capable of being brought into proximity to a plurality of electrode and counterelectrode pairs, said binding domains being capable of binding an analyte of interest, in which said contacting is under conditions such that binding of any analyte in the sample to said binding domains can occur, and in which said sample contains molecules that are linked to an electrochemiluminescent label;
(b) bringing said binding domains into proximity to said plurality of electrode and counterelectrode pairs;
(c) applying a voltage waveform effective to trigger electrochemiluminescence; and (d) detecting or measuring electrochemiluminescence, in which an increase in electrochemiluminescence over background levels indicates the presence or amount of the analyte in the sample.
132. A method of detecting or measuring an analyte of interest in a sample comprising:
(a) contacting a sample with a surface of a support, said surface containing a plurality of discrete binding domains, said binding domains being spatially aligned with and capable of being brought into proximity to a plurality of electrode and counterelectrode pairs, said binding domains being capable of binding an analyte of interest, in which said contacting is under conditions such that binding of any analyte in the sample to said binding domains can occur;
(b) contacting said binding domains with a binding partner of the analyte of interest, in which said binding partner is linked to an electrochemiluminescent label;
(c) bringing said binding domains into proximity to said plurality of electrode and counterelectrode pairs;
(d) applying a voltage waveform effective to trigger electrochemiluminescence; and (e) detecting or measuring electrochemiluminescence, in which an increase in electrochemiluminescence over background levels indicates the presence or amount of the analyte in the sample.
(a) contacting a sample with a surface of a support, said surface containing a plurality of discrete binding domains, said binding domains being spatially aligned with and capable of being brought into proximity to a plurality of electrode and counterelectrode pairs, said binding domains being capable of binding an analyte of interest, in which said contacting is under conditions such that binding of any analyte in the sample to said binding domains can occur;
(b) contacting said binding domains with a binding partner of the analyte of interest, in which said binding partner is linked to an electrochemiluminescent label;
(c) bringing said binding domains into proximity to said plurality of electrode and counterelectrode pairs;
(d) applying a voltage waveform effective to trigger electrochemiluminescence; and (e) detecting or measuring electrochemiluminescence, in which an increase in electrochemiluminescence over background levels indicates the presence or amount of the analyte in the sample.
133. The method according to claim 131 in which steps (a) and (b) are carried out concurrently.
134. The method according to claim 131 in which step (a) is carried out before step (b); and washing the binding domains to remove unbound analyte is carried out after step (a) and before step (b).
135. A method of detecting or measuring an analyte of interest in a sample comprising:
(a) contacting a first sample with a surface of a support, said surface containing a plurality of discrete binding domains, said binding domains being spatially aligned with and capable of being brought into proximity to a plurality of electrode and counterelectrode pairs, in which said sample contains an analyte of interest linked to an electrochemiluminescent label, in which said contacting is under conditions such that said analyte in the sample can bind to said binding domains;
(b) contacting a second sample with said binding domains, under conditions such that binding of any analyte in said second sample to said binding domains can occur, in which an electrochemiluminescent label is not linked to any analyte in said second sample;
(c) bringing said binding domains into proximity to said plurality of electrode and counterelectrode pairs;
(d) applying a voltage waveform effective to trigger electrochemiluminescence; and (e) detecting or measuring electrochemiluminescence, in which a decrease in electrochemiluminescence relative to the electrochemiluminescence observed when step (b) is omitted or when there is no analyte in the second sample, indicates the presence or amount of the analyte in the sample.
(a) contacting a first sample with a surface of a support, said surface containing a plurality of discrete binding domains, said binding domains being spatially aligned with and capable of being brought into proximity to a plurality of electrode and counterelectrode pairs, in which said sample contains an analyte of interest linked to an electrochemiluminescent label, in which said contacting is under conditions such that said analyte in the sample can bind to said binding domains;
(b) contacting a second sample with said binding domains, under conditions such that binding of any analyte in said second sample to said binding domains can occur, in which an electrochemiluminescent label is not linked to any analyte in said second sample;
(c) bringing said binding domains into proximity to said plurality of electrode and counterelectrode pairs;
(d) applying a voltage waveform effective to trigger electrochemiluminescence; and (e) detecting or measuring electrochemiluminescence, in which a decrease in electrochemiluminescence relative to the electrochemiluminescence observed when step (b) is omitted or when there is no analyte in the second sample, indicates the presence or amount of the analyte in the sample.
136. The method according to claim 131 in which the sample is derived from a mammal, and in which said method is used to determine or confirm the identity of the mammal.
137. The method according to claim 131 in which the sample contains cells from a mammal, and said method is used to quantitate the number of cells in said sample.
138. A method for performing a reaction of interest comprising:
(a) applying a sample to each of a plurality of discrete domains on the surface of a first support, each of said domains being hydrophilic and surrounded by a hydrophobic region on said first support surface; and (b) situating a second support having a plurality of discrete domains on the surface of the second support, each of said domains (i) being hydrophilic and surrounded by a hydrophobic region on said second support surface, (ii) comprising reaction medium suitable for performing a reaction of interest, and (iii) being spatially aligned with the domains on said first support surface, so as to bring each of said domains on said second support surface into contact with an aligned domain on said first support surface, such that said sample is contacted with said reaction medium.
(a) applying a sample to each of a plurality of discrete domains on the surface of a first support, each of said domains being hydrophilic and surrounded by a hydrophobic region on said first support surface; and (b) situating a second support having a plurality of discrete domains on the surface of the second support, each of said domains (i) being hydrophilic and surrounded by a hydrophobic region on said second support surface, (ii) comprising reaction medium suitable for performing a reaction of interest, and (iii) being spatially aligned with the domains on said first support surface, so as to bring each of said domains on said second support surface into contact with an aligned domain on said first support surface, such that said sample is contacted with said reaction medium.
139. The method according to claim 109 in which said plurality of fluid samples is delivered concurrently onto said monolayer.
140. The method according to claim 110 wherein said support comprises an elastomeric material.
141. The method according to claim 119 wherein said patterned self-assembled monolayer comprises alkane thiols.
142. A method for detecting or measuring an analyte of interest comprising:
(a) contacting one or more of a plurality of discrete binding domains, said plurality of binding domains situated on a surface of one or more supports, in which said contacting is with a sample comprising molecules linked to an electrochemiluminescent label, wherein said sample does not contact any electrodes or counterelectrodes during said contacting step;
(b) bringing an electrode into proximity to said one or more of a plurality of binding domains;
(c) applying a voltage waveform effective to trigger electrochemiluminescence at said one or more of a plurality of binding domains; and (d) detecting or measuring electrochemiluminescence.
(a) contacting one or more of a plurality of discrete binding domains, said plurality of binding domains situated on a surface of one or more supports, in which said contacting is with a sample comprising molecules linked to an electrochemiluminescent label, wherein said sample does not contact any electrodes or counterelectrodes during said contacting step;
(b) bringing an electrode into proximity to said one or more of a plurality of binding domains;
(c) applying a voltage waveform effective to trigger electrochemiluminescence at said one or more of a plurality of binding domains; and (d) detecting or measuring electrochemiluminescence.
143. A method for detecting or measuring an analyte of interest comprising:
(a) contacting one or more of a plurality of discrete binding domains, said plurality of binding domains (i) being situated on a surface of one or more supports, and (ii) being spatially aligned with and in proximity to a plurality of electrode and counterelectrode pairs, in which said contacting is with a sample comprising molecules linked to an electrochemiluminescent label;
(b) bringing an electrode and counterelectrode into proximity to said one or more of a plurality of binding domains;
(c) applying a voltage waveform effective to trigger electrochemiluminescence at said one or more of a plurality of binding domains; and (d) detecting or measuring electrochemiluminescence.
(a) contacting one or more of a plurality of discrete binding domains, said plurality of binding domains (i) being situated on a surface of one or more supports, and (ii) being spatially aligned with and in proximity to a plurality of electrode and counterelectrode pairs, in which said contacting is with a sample comprising molecules linked to an electrochemiluminescent label;
(b) bringing an electrode and counterelectrode into proximity to said one or more of a plurality of binding domains;
(c) applying a voltage waveform effective to trigger electrochemiluminescence at said one or more of a plurality of binding domains; and (d) detecting or measuring electrochemiluminescence.
144. A cassette comprising:
(a) an electrode containing on its surface a predetermined amount of a material comprising binding reagents organized into one or more discrete binding domains, said binding reagents being capable of specifically binding an electrochemiluminescent- labelled molecule or a binding partner of an electrochemiluminescent-labelled molecule, and said material (i) being permissive to electron transfer between said electrode and an electrochemiluminescent label in said electrochemiluminescent-labelled molecule bound to said binding reagent or to said binding partner when said binding partner is bound to said binding reagent, or (ii) allowing said electrode to generate photons from said electrochemiluminescent label; and (b) a counterelectrode.
(a) an electrode containing on its surface a predetermined amount of a material comprising binding reagents organized into one or more discrete binding domains, said binding reagents being capable of specifically binding an electrochemiluminescent- labelled molecule or a binding partner of an electrochemiluminescent-labelled molecule, and said material (i) being permissive to electron transfer between said electrode and an electrochemiluminescent label in said electrochemiluminescent-labelled molecule bound to said binding reagent or to said binding partner when said binding partner is bound to said binding reagent, or (ii) allowing said electrode to generate photons from said electrochemiluminescent label; and (b) a counterelectrode.
145. A cassette comprising an electrode containing on its surface a predetermined amount of a material comprising binding reagents organized into one or more discrete binding domains, said binding reagents being electrochemiluminescent-labelled, and said material being permissive to electron transfer between said electrode and said electrochemiluminescent label.
146. The cassette according to claim 143 in which said electrode is porous.
147. The cassette according to claim 144 in which said electrode is porous.
148. A cassette comprising:
(a) a support having one or more discrete binding domains containing binding reagents;
(b) an ionically permeable porous matrix, said binding domain being linked to said porous matrix;
(c) an electrode in electrochemical contact with said binding domain; and (d) a counterelectrode.
(a) a support having one or more discrete binding domains containing binding reagents;
(b) an ionically permeable porous matrix, said binding domain being linked to said porous matrix;
(c) an electrode in electrochemical contact with said binding domain; and (d) a counterelectrode.
149. The cassette according to claim 147 in which the binding reagents are electrochemiluminescent-labelled.
150. A cassette comprising:
(a) a binding domain containing binding reagents; and (b) a porous support, said binding domain being linked to said porous support, said porous support being an electrode in electrochemical contact with said binding domain.
(a) a binding domain containing binding reagents; and (b) a porous support, said binding domain being linked to said porous support, said porous support being an electrode in electrochemical contact with said binding domain.
151. The cassette according to claim 149 which further comprises a counterelectrode.
152. The cassette according to claim 150 wherein said binding reagents are covalently bound to said electrode.
153. A cassette comprising:
(a) a first support having a first surface containing an electrode; and (b) a second support having a second surface containing a binding domain, said first and second surfaces being spatially aligned to make electrochemical contact.
(a) a first support having a first surface containing an electrode; and (b) a second support having a second surface containing a binding domain, said first and second surfaces being spatially aligned to make electrochemical contact.
154. A kit comprising:
(a) a first support having a first surface containing an electrode;
(b) a second support having a second surface containing a binding domain, said binding domain containing a predetermined amount of binding reagents, said first and second surfaces spatially aligned to make electrochemical; contact; and (c) a vial containing an electrochemiluminescent labelled species.
(a) a first support having a first surface containing an electrode;
(b) a second support having a second surface containing a binding domain, said binding domain containing a predetermined amount of binding reagents, said first and second surfaces spatially aligned to make electrochemical; contact; and (c) a vial containing an electrochemiluminescent labelled species.
155. A cassette comprising:
(a) a first support having a first surface containing an electrode; and (b) a second support having a second surface containing one or more discrete binding domains, said first and second surfaces spatially aligned to allow electron transfer between an electrochemiluminescent label associated with the binding domain and the electrode.
(a) a first support having a first surface containing an electrode; and (b) a second support having a second surface containing one or more discrete binding domains, said first and second surfaces spatially aligned to allow electron transfer between an electrochemiluminescent label associated with the binding domain and the electrode.
156. The cassette according to claim 154 in which the second surface is a porous material, and which cassette further comprises a counterelectrode.
157. A cassette comprising:
(a) an electrode having a first surface;
(b) a counterelectrode having a second surface;
(c) a porous material between said first and second surfaces, said porous material containing binding reagents that are capable of binding an electrochemiluminescent-labelled molecule, said porous material and said first surface being spatially aligned so as to permit electron transfer between an electrochemiluminescent label on a molecule bound to one or more of said binding reagents and said first surface.
(a) an electrode having a first surface;
(b) a counterelectrode having a second surface;
(c) a porous material between said first and second surfaces, said porous material containing binding reagents that are capable of binding an electrochemiluminescent-labelled molecule, said porous material and said first surface being spatially aligned so as to permit electron transfer between an electrochemiluminescent label on a molecule bound to one or more of said binding reagents and said first surface.
158. A cassette comprising:
(a) a porous electrode;
(b) a counterelectrode;
(c) a support having a plurality of discrete binding domains thereon, said binding domains being spatially aligned to allow electron transfer between an electrochemiluminescent-labelled species linked to the binding domain and said porous electrode.
(a) a porous electrode;
(b) a counterelectrode;
(c) a support having a plurality of discrete binding domains thereon, said binding domains being spatially aligned to allow electron transfer between an electrochemiluminescent-labelled species linked to the binding domain and said porous electrode.
159. A cassette comprising:
(a) a porous electrode; and (b) a counterelectrode; said porous electrode containing on its surface a material comprising binding reagents, said material being permissive to electron transfer between said electrode and an electrochemiluminescent label bound to one or more of said binding reagents.
(a) a porous electrode; and (b) a counterelectrode; said porous electrode containing on its surface a material comprising binding reagents, said material being permissive to electron transfer between said electrode and an electrochemiluminescent label bound to one or more of said binding reagents.
160. A cassette comprising:
(a) a support having a plurality of discrete binding domains thereon;
(b) a compartment for fluid transport toward and away from said binding domains; and (c) an electrode and a counterelectrode spatially aligned so as to generate an electrochemiluminescent signal from an electrochemiluminescent label bound to said plurality of binding domains.
(a) a support having a plurality of discrete binding domains thereon;
(b) a compartment for fluid transport toward and away from said binding domains; and (c) an electrode and a counterelectrode spatially aligned so as to generate an electrochemiluminescent signal from an electrochemiluminescent label bound to said plurality of binding domains.
161. A cassette comprising:
(a) an electrode containing on its surface a plurality of discrete binding domains; and (b) a counterelectrode.
(a) an electrode containing on its surface a plurality of discrete binding domains; and (b) a counterelectrode.
162. A cassette comprising:
(a) a plurality of discrete binding domains on a support;
(b) an electrode; and (c) a counterelectrode, said electrode being capable of generating a plurality of electrochemiluminescent signals from a plurality of electrochemiluminescent labels linked to said plurality of discrete binding domains.
(a) a plurality of discrete binding domains on a support;
(b) an electrode; and (c) a counterelectrode, said electrode being capable of generating a plurality of electrochemiluminescent signals from a plurality of electrochemiluminescent labels linked to said plurality of discrete binding domains.
163. The cassette of claim 161 in which said plurality of binding domains contain an electrochemiluminescent label, and which cassette further comprises means for sample delivery onto said plurality of discrete binding domains.
164. The cassette of claim 161 which further comprises means for sample delivery onto said plurality of discrete binding domains.
165. The cassette of claim 160 in which said electrode is capable of generating an electrochemiluminescent signal from an electrochemiluminescent label associated with at least one of said binding domains.
166. A cassette comprising:
(a) a support having a plurality of discrete binding domains thereon; and (b) one or more electrode and counterelectrode pairs.
(a) a support having a plurality of discrete binding domains thereon; and (b) one or more electrode and counterelectrode pairs.
167. The cassette of claim 165 which further comprises means for sample delivery onto said plurality of discrete binding domains.
168. A kit comprising:
(a) an electrode containing on its surface discrete binding domains organized into a pattern;
(b) a compartment for fluid transport toward and from said binding domains;
(c) a counterelectrode; and (d) a vial containing electrochemiluminescent-labelled molecules.
(a) an electrode containing on its surface discrete binding domains organized into a pattern;
(b) a compartment for fluid transport toward and from said binding domains;
(c) a counterelectrode; and (d) a vial containing electrochemiluminescent-labelled molecules.
169. A cassette comprising:
(a) a plurality of discrete binding domains linked to an electrode, said electrode being proximal to an ionically permeable porous matrix; and (b) a counterelectrode.
(a) a plurality of discrete binding domains linked to an electrode, said electrode being proximal to an ionically permeable porous matrix; and (b) a counterelectrode.
170. A cassette comprising:
(a) a plurality of discrete binding domains, said binding domains being linked to an ionically permeable porous matrix;
(b) an electrode proximal to said ionically permeable porous matrix; and (c) a counterelectrode.
(a) a plurality of discrete binding domains, said binding domains being linked to an ionically permeable porous matrix;
(b) an electrode proximal to said ionically permeable porous matrix; and (c) a counterelectrode.
171. A cassette comprising:
(a) a plurality of discrete binding domains, said binding domains linked to an ionically permeable porous matrix; and (b) an electrode in electrochemical contact with a plurality of said binding domains.
(a) a plurality of discrete binding domains, said binding domains linked to an ionically permeable porous matrix; and (b) an electrode in electrochemical contact with a plurality of said binding domains.
172. The cassette of claim 170 which further comprises a counterelectrode.
173. A cassette comprising:
(a) a plurality of discrete binding domains, said plurality of binding domains linked to a porous support; and (b) an electrode in electrochemical contact with a plurality of said binding domains.
(a) a plurality of discrete binding domains, said plurality of binding domains linked to a porous support; and (b) an electrode in electrochemical contact with a plurality of said binding domains.
174. A cassette comprising:
(a) an electrochemical cell comprising an electrode, a counterelectrode and an ionic solution contacting said electrode; and (b) a support having a plurality of discrete binding domains thereon, said plurality of binding domains being in electrochemical contact with said electrode.
(a) an electrochemical cell comprising an electrode, a counterelectrode and an ionic solution contacting said electrode; and (b) a support having a plurality of discrete binding domains thereon, said plurality of binding domains being in electrochemical contact with said electrode.
175. The cassette of claim 143, 144, or 160 in which the electrode comprises carbon fibrils.
176. A method for detecting or measuring electrochemiluminescence comprising:
(a) contacting the binding reagents in the cassette of claim 143 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
(a) contacting the binding reagents in the cassette of claim 143 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
177. A method for detecting or measuring electrochemiluminescence comprising:
(a) contacting the binding reagents in the cassette of claim 144 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
(a) contacting the binding reagents in the cassette of claim 144 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
178. The method according to claim 175 in which said electrode is porous.
179. A method for detecting or measuring electrochemiluminescence comprising:
(a) contacting the binding reagents in the cassette of claim 147 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
(a) contacting the binding reagents in the cassette of claim 147 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
180. A method for detecting or measuring electrochemiluminescence comprising:
(a) contacting the binding reagents in the cassette of claim 150 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
(a) contacting the binding reagents in the cassette of claim 150 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
181. A method for detecting or measuring electrochemiluminescence comprising:
(a) contacting a binding domain contained on a first surface with a sample comprising molecules linked to an electrochemiluminscent label, said first surface aligned with a second surface containing an electrode, said first and second surfaces spatially aligned to make electrochemical contact;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and a counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
(a) contacting a binding domain contained on a first surface with a sample comprising molecules linked to an electrochemiluminscent label, said first surface aligned with a second surface containing an electrode, said first and second surfaces spatially aligned to make electrochemical contact;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and a counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
182. The method according to claim 180 in which the first surface is a porous material.
183. A method for detecting or measuring electrochemiluminescence comprising:
(a) contacting the binding reagents in the cassette of claim 156 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said first surface and said second surface; and (c) detecting or measuring said electrochemiluminescence.
(a) contacting the binding reagents in the cassette of claim 156 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said first surface and said second surface; and (c) detecting or measuring said electrochemiluminescence.
184 184. A method for detecting or measuring electrochemiluminescence comprising:
(a) contacting the binding domains in the cassette of claim 157 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
(a) contacting the binding domains in the cassette of claim 157 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
185. A method for detecting or measuring electrochemiluminescence comprising:
(a) contacting the binding reagents in the cassette of claim 158 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode surface and counterelectrode surface; and (c) detecting or measuring said electrochemiluminescence.
(a) contacting the binding reagents in the cassette of claim 158 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode surface and counterelectrode surface; and (c) detecting or measuring said electrochemiluminescence.
186. A method for detecting or measuring electrochemiluminescence comprising:
(a) contacting the binding domains in the cassette of claim 159 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode surface and counterelectrode surface; and (c) detecting or measuring said electrochemiluminescence.
(a) contacting the binding domains in the cassette of claim 159 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode surface and counterelectrode surface; and (c) detecting or measuring said electrochemiluminescence.
187. A method for detecting or measuring electrochemiluminescence comprising:
(a) contacting the binding domains in the cassette of claim 160 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
(a) contacting the binding domains in the cassette of claim 160 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
188. A method for detecting or measuring electrochemiluminescence comprising:
(a) contacting the binding domains in the cassette of claim 161 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
(a) contacting the binding domains in the cassette of claim 161 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
189. A method for detecting or measuring electrochemiluminescence comprising:
(a) contacting a plurality of discrete binding domains on a support with a sample comprising molecules linked to an electrochemiluminscent label by delivery of a sample containing said molecules onto said binding domains, said sample being capable of modulating electrochemiluminscent signals, in which said modulating is correlatable to a concentration of an analyte of interest in the sample;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at an electrode and counterelectrode from an electrochemiluminscent label linked to a molecule bound to at least one of said binding domains;
(c) measuring said electrochemiluminescence; and (d) determining said concentration of the analyte of interest.
(a) contacting a plurality of discrete binding domains on a support with a sample comprising molecules linked to an electrochemiluminscent label by delivery of a sample containing said molecules onto said binding domains, said sample being capable of modulating electrochemiluminscent signals, in which said modulating is correlatable to a concentration of an analyte of interest in the sample;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at an electrode and counterelectrode from an electrochemiluminscent label linked to a molecule bound to at least one of said binding domains;
(c) measuring said electrochemiluminescence; and (d) determining said concentration of the analyte of interest.
190. A method for detecting or measuring electrochemiluminescence comprising:
(a) contacting the binding reagents in the cassette of claim 143 with a sample comprising a first molecule and a second molecule linked to an electrochemiluminscent label, in which the second molecule is a specific binding partner of the first molecule;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
(a) contacting the binding reagents in the cassette of claim 143 with a sample comprising a first molecule and a second molecule linked to an electrochemiluminscent label, in which the second molecule is a specific binding partner of the first molecule;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
191. A method for detecting or measuring electrochemiluminescence comprising:
(a) contacting the binding domains in the cassette of claim 165 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at at least one of said electrode and counterelectrode pairs; and (c) detecting or measuring said electrochemiluminescence.
(a) contacting the binding domains in the cassette of claim 165 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at at least one of said electrode and counterelectrode pairs; and (c) detecting or measuring said electrochemiluminescence.
192. A method for detecting or measuring electrochemiluminescence comprising:
(a) contacting the binding domains in the cassette of claim 168 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
(a) contacting the binding domains in the cassette of claim 168 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
193. A method for detecting or measuring electrochemiluminescence comprising:
(a) contacting the binding domains in the cassette of claim 169 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
(a) contacting the binding domains in the cassette of claim 169 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
194. A method for detecting or measuring electrochemiluminescence comprising:
(a) contacting the binding domains in the cassette of claim 171 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
(a) contacting the binding domains in the cassette of claim 171 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
195. A method for detecting or measuring electrochemiluminescence comprising:
(a) contacting the binding domains in the cassette of claim 173 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
(a) contacting the binding domains in the cassette of claim 173 with a sample comprising molecules linked to an electrochemiluminescent label;
(b) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode and counterelectrode; and (c) detecting or measuring said electrochemiluminescence.
196. The method according to claim 175, 188, or 189 in which the electrode comprises carbon fibrils.
197. A method for conducting a plurality of electrochemiluminescence assays comprising the steps of:
(a) contacting a plurality of discrete polymer matrices, each polymer matrix comprising one or more binding domains with a sample containing one or a plurality of analytes of interest;
(b) causing one or more of said plurality of domains to electrochemiluminesce;
(c) detecting electrochemiluminescence.
(a) contacting a plurality of discrete polymer matrices, each polymer matrix comprising one or more binding domains with a sample containing one or a plurality of analytes of interest;
(b) causing one or more of said plurality of domains to electrochemiluminesce;
(c) detecting electrochemiluminescence.
198. A method as recited in claim 197 wherein electrochemiluminesce is detected by photographic film.
199. A method for conducting a plurality of electrochemiluminescence assays comprising the steps of:
(a) contacting a plurality of binding domains comprising oligonucleic acids with a sample containing one or a plurality of analytes of interest;
(b) causing one or more of said plurality of domains to electrochemiluminesce; and (c) detecting electrochemiluminescence at a plurality of said binding domains.
(a) contacting a plurality of binding domains comprising oligonucleic acids with a sample containing one or a plurality of analytes of interest;
(b) causing one or more of said plurality of domains to electrochemiluminesce; and (c) detecting electrochemiluminescence at a plurality of said binding domains.
200. A method as recited in claim 199 wherein said plurality of binding domains contain oligonucleic acids linked to electrochemiluminescent labels.
201. A method as recited in claim 199 wherein said plurality of binding domains contain oligonucleic acids linked to electrochemiluminescent labels and one or more of said analytes, when bound to said oligonucleic acids, causes modulation of the electrochemiluminescent signal from one or more binding domains.
202. A method for conducting a plurality of electrochemiluminescence assays comprising detecting a plurality of analytes present in a multicomponent sample at a concentration below about 10-3 comprising the steps of:
(a) contacting a plurality of discrete binding domains with a sample containing one or a plurality of analytes of interest;
(b) causing one or more of said plurality of domains to electrochemiluminesce; and (c) detecting electrochemiluminescence at a plurality of said binding domains.
(a) contacting a plurality of discrete binding domains with a sample containing one or a plurality of analytes of interest;
(b) causing one or more of said plurality of domains to electrochemiluminesce; and (c) detecting electrochemiluminescence at a plurality of said binding domains.
203. A system for conducting a plurality of electrochemiluminescence assays comprising:
(a) a plurality of binding domains specific for a plurality of different analytes;
(b) a voltage waveform generator; and (c) a photon detection means.
(a) a plurality of binding domains specific for a plurality of different analytes;
(b) a voltage waveform generator; and (c) a photon detection means.
204. A system for conducting a plurality of electrochemiluminescence assays comprising:
(a) an electrode;
(b) a counterelectrode;
(c) a plurality of binding domains specific for a plurality of different analytes;
(d) means for triggering electrochemiluminescence; and (e) means for electrochemiluminescence detection.
(a) an electrode;
(b) a counterelectrode;
(c) a plurality of binding domains specific for a plurality of different analytes;
(d) means for triggering electrochemiluminescence; and (e) means for electrochemiluminescence detection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA2704228A CA2704228C (en) | 1995-03-10 | 1996-03-06 | Multi-array, multi-specific electrochemiluminescence testing |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US40227795A | 1995-03-10 | 1995-03-10 | |
| US40207695A | 1995-03-10 | 1995-03-10 | |
| US08/402,277 | 1995-03-10 | ||
| US08/402,076 | 1995-03-10 | ||
| PCT/US1996/003190 WO1996028538A1 (en) | 1995-03-10 | 1996-03-06 | Multi-array, multi-specific electrochemiluminescence testing |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2704228A Division CA2704228C (en) | 1995-03-10 | 1996-03-06 | Multi-array, multi-specific electrochemiluminescence testing |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2213854A1 true CA2213854A1 (en) | 1996-09-19 |
| CA2213854C CA2213854C (en) | 2010-08-10 |
Family
ID=27017707
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2704228A Expired - Lifetime CA2704228C (en) | 1995-03-10 | 1996-03-06 | Multi-array, multi-specific electrochemiluminescence testing |
| CA2213854A Expired - Lifetime CA2213854C (en) | 1995-03-10 | 1996-03-06 | Multi-array, multi-specific electrochemiluminescence testing |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2704228A Expired - Lifetime CA2704228C (en) | 1995-03-10 | 1996-03-06 | Multi-array, multi-specific electrochemiluminescence testing |
Country Status (15)
| Country | Link |
|---|---|
| US (2) | US6066448A (en) |
| EP (2) | EP2280268B1 (en) |
| JP (4) | JPH11502617A (en) |
| KR (1) | KR100449615B1 (en) |
| CN (2) | CN1661115A (en) |
| AU (1) | AU720625B2 (en) |
| BR (1) | BR9607193B1 (en) |
| CA (2) | CA2704228C (en) |
| CZ (1) | CZ299135B6 (en) |
| EA (1) | EA001198B1 (en) |
| HK (1) | HK1151588A1 (en) |
| HU (1) | HUP9801679A3 (en) |
| NZ (1) | NZ306051A (en) |
| TW (1) | TW555852B (en) |
| WO (1) | WO1996028538A1 (en) |
Families Citing this family (503)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5965452A (en) | 1996-07-09 | 1999-10-12 | Nanogen, Inc. | Multiplexed active biologic array |
| US5824473A (en) * | 1993-12-10 | 1998-10-20 | California Institute Of Technology | Nucleic acid mediated electron transfer |
| US5620850A (en) | 1994-09-26 | 1997-04-15 | President And Fellows Of Harvard College | Molecular recognition at surfaces derivatized with self-assembled monolayers |
| US20040202603A1 (en) * | 1994-12-08 | 2004-10-14 | Hyperion Catalysis International, Inc. | Functionalized nanotubes |
| US6852502B1 (en) * | 1995-06-06 | 2005-02-08 | Bioveris Corporation | Electrochemiluminescent enzyme biosensors |
| US6207369B1 (en) * | 1995-03-10 | 2001-03-27 | Meso Scale Technologies, Llc | Multi-array, multi-specific electrochemiluminescence testing |
| CN1661115A (en) * | 1995-03-10 | 2005-08-31 | 梅索磅秤技术有限公司 | Multi-array, multi-specific electrochemiluminescence testing |
| US6673533B1 (en) | 1995-03-10 | 2004-01-06 | Meso Scale Technologies, Llc. | Multi-array multi-specific electrochemiluminescence testing |
| US6319670B1 (en) | 1995-05-09 | 2001-11-20 | Meso Scale Technology Llp | Methods and apparatus for improved luminescence assays using microparticles |
| US6387707B1 (en) * | 1996-04-25 | 2002-05-14 | Bioarray Solutions | Array Cytometry |
| DE69737883T2 (en) | 1996-04-25 | 2008-03-06 | Bioarray Solutions Ltd. | LIGHT-REGULATED, ELECTROKINETIC COMPOSITION OF PARTICLES TO SURFACES |
| US7041510B2 (en) | 1996-04-25 | 2006-05-09 | Bioarray Solutions Ltd. | System and method for programmable illumination pattern generation |
| US7144119B2 (en) * | 1996-04-25 | 2006-12-05 | Bioarray Solutions Ltd. | System and method for programmable illumination pattern generation |
| US6958245B2 (en) | 1996-04-25 | 2005-10-25 | Bioarray Solutions Ltd. | Array cytometry |
| SE9602545L (en) * | 1996-06-25 | 1997-12-26 | Michael Mecklenburg | Method of discriminating complex biological samples |
| US6506564B1 (en) * | 1996-07-29 | 2003-01-14 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
| US6582921B2 (en) * | 1996-07-29 | 2003-06-24 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses thereof |
| EP1249705A3 (en) * | 1996-12-31 | 2003-11-05 | Genometrix Genomics Incorporated | Multiplexed molecular analysis apparatus and its fabrication method |
| SE9700384D0 (en) * | 1997-02-04 | 1997-02-04 | Biacore Ab | Analytical method and apparatus |
| US7885697B2 (en) | 2004-07-13 | 2011-02-08 | Dexcom, Inc. | Transcutaneous analyte sensor |
| US6862465B2 (en) | 1997-03-04 | 2005-03-01 | Dexcom, Inc. | Device and method for determining analyte levels |
| US8527026B2 (en) | 1997-03-04 | 2013-09-03 | Dexcom, Inc. | Device and method for determining analyte levels |
| US7657297B2 (en) | 2004-05-03 | 2010-02-02 | Dexcom, Inc. | Implantable analyte sensor |
| US7899511B2 (en) | 2004-07-13 | 2011-03-01 | Dexcom, Inc. | Low oxygen in vivo analyte sensor |
| US6001067A (en) | 1997-03-04 | 1999-12-14 | Shults; Mark C. | Device and method for determining analyte levels |
| US7622294B2 (en) | 1997-03-14 | 2009-11-24 | Trustees Of Tufts College | Methods for detecting target analytes and enzymatic reactions |
| US20030027126A1 (en) | 1997-03-14 | 2003-02-06 | Walt David R. | Methods for detecting target analytes and enzymatic reactions |
| US6872527B2 (en) * | 1997-04-16 | 2005-03-29 | Xtrana, Inc. | Nucleic acid archiving |
| AUPO625497A0 (en) * | 1997-04-16 | 1997-05-15 | Macquarie Research Limited | Analysis of molecules |
| DE69836587T2 (en) * | 1997-04-16 | 2007-10-11 | Applera Corp., Foster City | NUCLEIC COLLECTION |
| AU756945B2 (en) | 1997-05-23 | 2003-01-30 | Bioarray Solutions Ltd | Color-encoding and in-situ interrogation of matrix-coupled chemical compounds |
| ATE498838T1 (en) * | 1997-06-12 | 2011-03-15 | Clinical Micro Sensors Inc | ELECTRONIC METHOD AND DEVICE FOR DETECTING ANALYTES |
| US6013459A (en) | 1997-06-12 | 2000-01-11 | Clinical Micro Sensors, Inc. | Detection of analytes using reorganization energy |
| FR2765967B1 (en) * | 1997-07-11 | 1999-08-20 | Commissariat Energie Atomique | CHIP ANALYSIS DEVICE COMPRISING LOCALIZED HEATING ELECTRODES |
| US6368871B1 (en) * | 1997-08-13 | 2002-04-09 | Cepheid | Non-planar microstructures for manipulation of fluid samples |
| US7348181B2 (en) | 1997-10-06 | 2008-03-25 | Trustees Of Tufts College | Self-encoding sensor with microspheres |
| DE19751658A1 (en) * | 1997-11-21 | 1999-07-29 | Wolfbeis Otto S Prof Dr | Process for the formation of laterally organized structures on support surfaces |
| CA2312102C (en) | 1997-12-24 | 2007-09-04 | Cepheid | Integrated fluid manipulation cartridge |
| WO1999040434A1 (en) | 1998-02-04 | 1999-08-12 | Invitrogen Corporation | Microarrays and uses therefor |
| US7297312B2 (en) * | 1998-03-16 | 2007-11-20 | University Of Cincinnati | Simultaneous multianalyte electrochemical assay based on spatial resolution |
| DE19811582A1 (en) * | 1998-03-17 | 1999-09-23 | Roche Diagnostics Gmbh | Electrochemiluminescence assay, especially for biological substances, with long measurement period |
| US6949816B2 (en) | 2003-04-21 | 2005-09-27 | Motorola, Inc. | Semiconductor component having first surface area for electrically coupling to a semiconductor chip and second surface area for electrically coupling to a substrate, and method of manufacturing same |
| US8465425B2 (en) | 1998-04-30 | 2013-06-18 | Abbott Diabetes Care Inc. | Analyte monitoring device and methods of use |
| US8974386B2 (en) | 1998-04-30 | 2015-03-10 | Abbott Diabetes Care Inc. | Analyte monitoring device and methods of use |
| US8480580B2 (en) | 1998-04-30 | 2013-07-09 | Abbott Diabetes Care Inc. | Analyte monitoring device and methods of use |
| US6175752B1 (en) | 1998-04-30 | 2001-01-16 | Therasense, Inc. | Analyte monitoring device and methods of use |
| US8346337B2 (en) | 1998-04-30 | 2013-01-01 | Abbott Diabetes Care Inc. | Analyte monitoring device and methods of use |
| US8688188B2 (en) | 1998-04-30 | 2014-04-01 | Abbott Diabetes Care Inc. | Analyte monitoring device and methods of use |
| US9066695B2 (en) | 1998-04-30 | 2015-06-30 | Abbott Diabetes Care Inc. | Analyte monitoring device and methods of use |
| US6200531B1 (en) * | 1998-05-11 | 2001-03-13 | Igen International, Inc. | Apparatus for carrying out electrochemiluminescence test measurements |
| GB9815042D0 (en) * | 1998-07-10 | 1998-09-09 | Imperial College | Detector |
| US6576478B1 (en) * | 1998-07-14 | 2003-06-10 | Zyomyx, Inc. | Microdevices for high-throughput screening of biomolecules |
| US6897073B2 (en) * | 1998-07-14 | 2005-05-24 | Zyomyx, Inc. | Non-specific binding resistant protein arrays and methods for making the same |
| US6406921B1 (en) * | 1998-07-14 | 2002-06-18 | Zyomyx, Incorporated | Protein arrays for high-throughput screening |
| US6780582B1 (en) * | 1998-07-14 | 2004-08-24 | Zyomyx, Inc. | Arrays of protein-capture agents and methods of use thereof |
| US20030138973A1 (en) * | 1998-07-14 | 2003-07-24 | Peter Wagner | Microdevices for screening biomolecules |
| US20020119579A1 (en) * | 1998-07-14 | 2002-08-29 | Peter Wagner | Arrays devices and methods of use thereof |
| US6521182B1 (en) * | 1998-07-20 | 2003-02-18 | Lifescan, Inc. | Fluidic device for medical diagnostics |
| US6830934B1 (en) * | 1999-06-15 | 2004-12-14 | Lifescan, Inc. | Microdroplet dispensing for a medical diagnostic device |
| FR2781886B1 (en) * | 1998-07-31 | 2001-02-16 | Commissariat Energie Atomique | MULTIPLE POINT CHEMICAL OR BIOLOGICAL ANALYSIS MICROSYSTEM |
| DE19940749A1 (en) | 1998-08-28 | 2000-05-18 | Febit Ferrarius Biotech Gmbh | Integrated synthesis and analysis method e.g. for polymers, comprises a carrier body provided with immobilized receptors to provide respective channels before contact with sample and subsequent analysis |
| FR2783179B1 (en) * | 1998-09-16 | 2000-10-06 | Commissariat Energie Atomique | CHEMICAL OR BIOLOGICAL ANALYSIS DEVICE COMPRISING A PLURALITY OF ANALYSIS SITES ON A MEDIUM, AND ITS MANUFACTURING METHOD |
| FR2784466B1 (en) * | 1998-10-13 | 2002-10-18 | Commissariat Energie Atomique | MULTIPLE POINT CHEMICAL OR BIOLOGICAL ANALYSIS MICROSYSTEM USING A COUPLING BETWEEN THE PROBES AND A SUBSTRATE |
| IL126776A (en) * | 1998-10-27 | 2001-04-30 | Technion Res & Dev Foundation | Method for gold depositions |
| US7914994B2 (en) * | 1998-12-24 | 2011-03-29 | Cepheid | Method for separating an analyte from a sample |
| US6431476B1 (en) | 1999-12-21 | 2002-08-13 | Cepheid | Apparatus and method for rapid ultrasonic disruption of cells or viruses |
| US6833267B1 (en) * | 1998-12-30 | 2004-12-21 | Clinical Micro Sensors, Inc. | Tissue collection devices containing biosensors |
| US6251685B1 (en) * | 1999-02-18 | 2001-06-26 | Agilent Technologies, Inc. | Readout method for molecular biological electronically addressable arrays |
| US6300143B1 (en) * | 1999-03-01 | 2001-10-09 | Idec Pharmaceuticals Corporation | Electrochemiluminescent assays for eukaryotic cells |
| US6280697B1 (en) * | 1999-03-01 | 2001-08-28 | The University Of North Carolina-Chapel Hill | Nanotube-based high energy material and method |
| US7312087B2 (en) * | 2000-01-11 | 2007-12-25 | Clinical Micro Sensors, Inc. | Devices and methods for biochip multiplexing |
| US6942771B1 (en) | 1999-04-21 | 2005-09-13 | Clinical Micro Sensors, Inc. | Microfluidic systems in the electrochemical detection of target analytes |
| US6127129A (en) * | 1999-05-04 | 2000-10-03 | Wisconsin Alumni Research Foundation | Process to create biomolecule and/or cellular arrays on metal surfaces and product produced thereby |
| US20040200909A1 (en) * | 1999-05-28 | 2004-10-14 | Cepheid | Apparatus and method for cell disruption |
| US8815521B2 (en) | 2000-05-30 | 2014-08-26 | Cepheid | Apparatus and method for cell disruption |
| JP4078073B2 (en) | 1999-05-28 | 2008-04-23 | シーフィード | Fluid sample analysis apparatus and method |
| US9073053B2 (en) | 1999-05-28 | 2015-07-07 | Cepheid | Apparatus and method for cell disruption |
| US6987602B2 (en) * | 1999-06-07 | 2006-01-17 | Research Frontiers Incorporated | Anisometrically shaped carbon and/or graphite particles, liquid suspensions and films thereof and light valves comprising same |
| US6878540B2 (en) * | 1999-06-25 | 2005-04-12 | Cepheid | Device for lysing cells, spores, or microorganisms |
| US6673561B1 (en) | 1999-06-30 | 2004-01-06 | Regents Of The University Of California | Diagnostic test for thrombotic or thromboembolic disease |
| EP1218541B1 (en) | 1999-07-26 | 2008-12-10 | Clinical Micro Sensors, Inc. | Sequence determination of nucleic acids using electronic detection |
| WO2001007892A1 (en) * | 1999-07-27 | 2001-02-01 | Esperion Therapeutics, Inc. | Method and device for measurement of cholesterol efflux |
| AU6504900A (en) * | 1999-07-30 | 2001-02-19 | Penn State Research Foundation, The | Instruments, methods and reagents for surface plasmon resonance |
| US6136268A (en) * | 1999-08-17 | 2000-10-24 | Orion Diagnostica | Method for luminescence measurements |
| AU7730500A (en) * | 1999-09-30 | 2001-04-30 | Nanogen, Inc. | Biomolecular attachment sites on microelectronic arrays and methods thereof |
| US6340822B1 (en) * | 1999-10-05 | 2002-01-22 | Agere Systems Guardian Corp. | Article comprising vertically nano-interconnected circuit devices and method for making the same |
| JP2001133436A (en) * | 1999-11-01 | 2001-05-18 | Microtec Nition:Kk | Matrix type substrate for electrochemical emission |
| JP2001153870A (en) * | 1999-11-25 | 2001-06-08 | Hitachi Software Eng Co Ltd | Hybridization device, case, supporting body, and labeled reagent |
| US6303082B1 (en) * | 1999-12-15 | 2001-10-16 | Nanogen, Inc. | Permeation layer attachment chemistry and method |
| WO2001050131A1 (en) * | 2000-01-06 | 2001-07-12 | Motorola, Inc. | Three-dimensional network for biomolecule detection |
| US6436055B1 (en) * | 2000-03-02 | 2002-08-20 | The Procter & Gamble Company | Device having diarrhea diagnostic panel |
| BR0109125A (en) * | 2000-03-09 | 2002-11-26 | Clinical Analysis Corp | Medical diagnostic system |
| US7102024B1 (en) | 2000-08-01 | 2006-09-05 | Schwartz David A | Functional biopolymer modification reagents and uses thereof |
| US6686461B1 (en) | 2000-03-22 | 2004-02-03 | Solulink Bioscience, Inc. | Triphosphate oligonucleotide modification reagents and uses thereof |
| DE10020704B4 (en) * | 2000-04-27 | 2006-09-28 | Bioref Gmbh | Biochip for archiving and laboratory medical analysis of biological sample material, process for its production and its use in diagnostic procedures |
| CA2344946A1 (en) * | 2000-05-10 | 2001-11-10 | Symyx Technologies, Inc. | Polymer libraries on a substrate, method of forming polymer libraries on a substrate and characterization methods with same |
| US9709559B2 (en) | 2000-06-21 | 2017-07-18 | Bioarray Solutions, Ltd. | Multianalyte molecular analysis using application-specific random particle arrays |
| JP4744778B2 (en) | 2000-06-21 | 2011-08-10 | バイオアレイ ソルーションズ リミテッド | Method for analyzing multiple analyte molecules using a specific random particle array |
| EP1309720B1 (en) * | 2000-07-11 | 2009-09-16 | Chesapeake Techlabs, Inc. | Apparatus and method for detecting and classifying chemicals, particles, viruses, and bacteria in fluids with an aptamer |
| DE10035451C2 (en) * | 2000-07-19 | 2002-12-05 | November Ag Molekulare Medizin | Method and device for identifying a polymer sequence |
| US6564079B1 (en) | 2000-07-27 | 2003-05-13 | Ckm Diagnostics, Inc. | Electrode array and skin attachment system for noninvasive nerve location and imaging device |
| WO2002010373A2 (en) * | 2000-07-31 | 2002-02-07 | Applera Corporation | Apparatus and method for specific release of captured extension products |
| KR100984585B1 (en) | 2000-08-22 | 2010-09-30 | 프레지던트 앤드 펠로우즈 오브 하버드 칼리지 | Nanosensors |
| US7057704B2 (en) * | 2000-09-17 | 2006-06-06 | Bioarray Solutions Ltd. | System and method for programmable illumination pattern generation |
| US6632872B1 (en) | 2000-09-19 | 2003-10-14 | 3M Innovative Properties Company | Adhesive compositions including self-assembling molecules, adhesives, articles, and methods |
| US6887714B2 (en) * | 2000-10-16 | 2005-05-03 | Board Of Trustees Of The University Of Arkansas, N.A. | Microvolume immunoabsorbant assays with amplified electrochemical detection |
| DE10051396A1 (en) * | 2000-10-17 | 2002-04-18 | Febit Ferrarius Biotech Gmbh | An integrated synthesis and identification of an analyte, comprises particles immobilized at a carrier to be coupled to receptors in a structured pattern to give receptor arrays for biochemical reactions |
| US20030045005A1 (en) * | 2000-10-17 | 2003-03-06 | Michael Seul | Light-controlled electrokinetic assembly of particles near surfaces |
| US20020051995A1 (en) * | 2000-10-30 | 2002-05-02 | Rajan Kumar | Stacked arrays |
| EP1332000B1 (en) * | 2000-10-30 | 2012-06-20 | Sequenom, Inc. | Method for delivery of submicroliter volumes onto a substrate |
| US7351575B2 (en) * | 2000-11-08 | 2008-04-01 | Surface Logix, Inc. | Methods for processing biological materials using peelable and resealable devices |
| US6803205B2 (en) * | 2000-11-08 | 2004-10-12 | Surface Logix, Inc. | Methods of measuring enzyme activity using peelable and resealable devices |
| US7001740B2 (en) * | 2000-11-08 | 2006-02-21 | Surface Logix, Inc. | Methods of arraying biological materials using peelable and resealable devices |
| US7439056B2 (en) | 2000-11-08 | 2008-10-21 | Surface Logix Inc. | Peelable and resealable devices for arraying materials |
| GB2368903A (en) * | 2000-11-08 | 2002-05-15 | Proimmune Ltd | Analysis of biological and biochemical assays |
| US7371563B2 (en) * | 2000-11-08 | 2008-05-13 | Surface Logix, Inc. | Peelable and resealable devices for biochemical assays |
| US6967074B2 (en) | 2000-11-08 | 2005-11-22 | Surface Logix, Inc. | Methods of detecting immobilized biomolecules |
| DE20119542U1 (en) * | 2000-11-30 | 2002-04-18 | Inst Physikalische Hochtech Ev | Arrangement for the production, testing and archiving of solid phase-bound chemical or biological libraries |
| KR20090049095A (en) | 2000-12-11 | 2009-05-15 | 프레지던트 앤드 펠로우즈 오브 하버드 칼리지 | Nanosensors |
| US6560471B1 (en) | 2001-01-02 | 2003-05-06 | Therasense, Inc. | Analyte monitoring device and methods of use |
| WO2002063270A2 (en) * | 2001-02-05 | 2002-08-15 | Board Of Regents, The University Of Texas System | The use of mesoscale self-assembly and recognition to effect delivery of sensing reagent for arrayed sensors |
| JP3693578B2 (en) * | 2001-02-13 | 2005-09-07 | 学校法人 東洋大学 | High-sensitivity analysis method for trace proteins |
| US20060108218A1 (en) * | 2001-03-05 | 2006-05-25 | Clinical Analysis Corporation | Test cell for use with medical diagnostic instrument |
| US6765190B2 (en) * | 2001-03-14 | 2004-07-20 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Multi-element electron-transfer optical detector system |
| US6750438B2 (en) * | 2001-03-14 | 2004-06-15 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Single-element electron-transfer optical detector system |
| US7041468B2 (en) | 2001-04-02 | 2006-05-09 | Therasense, Inc. | Blood glucose tracking apparatus and methods |
| US6740403B2 (en) | 2001-04-02 | 2004-05-25 | Toyo Tanso Co., Ltd. | Graphitic polyhederal crystals in the form of nanotubes, whiskers and nanorods, methods for their production and uses thereof |
| US20020160427A1 (en) * | 2001-04-27 | 2002-10-31 | Febit Ag | Methods and apparatuses for electronic determination of analytes |
| DE60221240T2 (en) * | 2001-05-02 | 2007-10-31 | Applera Corp., Foster City | CONCENTRATION AND CLEANING OF ANALYTES WITH ELECTRIC FIELDS |
| CA2446417A1 (en) * | 2001-05-03 | 2002-11-14 | Sigma Genosys, L.P. | Methods for assembling protein microarrays |
| US7132275B2 (en) * | 2001-05-14 | 2006-11-07 | The John Hopkins University | Multifunctional magnetic nanowires |
| US6723299B1 (en) * | 2001-05-17 | 2004-04-20 | Zyvex Corporation | System and method for manipulating nanotubes |
| US7189366B2 (en) * | 2001-05-25 | 2007-03-13 | The United States Of America As Represented By The Secretary Of The Interior | Molecular tag reader |
| DE10126798A1 (en) * | 2001-06-01 | 2002-12-19 | Nanotype Gmbh | Highly parallel determination of analytes, useful e.g. for diagnostic detection of proteins, by immobilization then contacting with surface carrying specific probes |
| US20020197474A1 (en) * | 2001-06-06 | 2002-12-26 | Reynolds Thomas A. | Functionalized fullerenes, their method of manufacture and uses thereof |
| US6749826B2 (en) | 2001-06-13 | 2004-06-15 | The Regents Of The University Of California | Carbon nanotube coatings as chemical absorbers |
| US7262063B2 (en) | 2001-06-21 | 2007-08-28 | Bio Array Solutions, Ltd. | Directed assembly of functional heterostructures |
| CA2451789C (en) | 2001-06-29 | 2012-03-27 | Meso Scale Technologies, Llc. | Assay plates, reader systems and methods for luminescence test measurements |
| AU2012205147B2 (en) * | 2001-06-29 | 2016-03-17 | Meso Scale Technologies, Llc | Assay plates reader systems and methods for luminescence test measurements |
| US7704728B2 (en) * | 2001-07-18 | 2010-04-27 | The University Of Michigan | Microfluidic gravity pump with constant flow rate |
| US20030032874A1 (en) | 2001-07-27 | 2003-02-13 | Dexcom, Inc. | Sensor head for use with implantable devices |
| AU2002365119A1 (en) * | 2001-07-30 | 2003-09-04 | Meso Scale Technologies, Llc. | Assay electrodes having immobilized lipid/protein layers and methods of making and using the same |
| KR100455284B1 (en) * | 2001-08-14 | 2004-11-12 | 삼성전자주식회사 | High-throughput sensor for detecting biomolecules using carbon nanotubes |
| US10539561B1 (en) | 2001-08-30 | 2020-01-21 | Customarray, Inc. | Enzyme-amplified redox microarray detection process |
| WO2003022028A2 (en) * | 2001-09-10 | 2003-03-20 | Meso Scale Technologies, Llc | Methods, reagents, kits and apparatus for protein function analysis |
| CA3081228C (en) | 2001-09-10 | 2022-02-15 | Meso Scale Technologies, Llc | Assay buffer, compositions containing the same, and methods of using the same |
| GB0124338D0 (en) * | 2001-10-10 | 2001-11-28 | Randox Lab Ltd | Biochips |
| JP4377689B2 (en) | 2001-10-15 | 2009-12-02 | バイオアレイ ソリューションズ リミテッド | Combined analysis of polymorphic loci with simultaneous interrogation and enzyme-mediated detection |
| US7074519B2 (en) * | 2001-10-26 | 2006-07-11 | The Regents Of The University Of California | Molehole embedded 3-D crossbar architecture used in electrochemical molecular memory device |
| US20030175947A1 (en) * | 2001-11-05 | 2003-09-18 | Liu Robin Hui | Enhanced mixing in microfluidic devices |
| US6960298B2 (en) * | 2001-12-10 | 2005-11-01 | Nanogen, Inc. | Mesoporous permeation layers for use on active electronic matrix devices |
| DE10162188A1 (en) * | 2001-12-17 | 2003-06-18 | Sunyx Surface Nanotechnologies | Apparatus to manipulate the smallest droplets has a screen pattern of electrodes, with a control system to apply an individual voltage to selected electrodes for a given time span to set the droplet movement path and speed |
| KR100408871B1 (en) * | 2001-12-20 | 2003-12-11 | 삼성전자주식회사 | Method of separation or filtration by carbon nanotube in biochip |
| US7244393B2 (en) * | 2001-12-21 | 2007-07-17 | Kimberly-Clark Worldwide, Inc. | Diagnostic device and system |
| DE60225593T2 (en) * | 2001-12-21 | 2009-04-16 | Ge Healthcare Bio-Sciences Ab | IMMOBILIZATION OF BINDING MATERIALS |
| JPWO2003055973A1 (en) * | 2001-12-26 | 2005-05-12 | オリンパス株式会社 | Reaction vessel and reaction vessel holding mechanism |
| DE10164358C2 (en) * | 2001-12-28 | 2003-11-27 | Advalytix Ag | Characterization process for functionalized surfaces |
| US8152991B2 (en) * | 2005-10-27 | 2012-04-10 | Nanomix, Inc. | Ammonia nanosensors, and environmental control system |
| US8154093B2 (en) | 2002-01-16 | 2012-04-10 | Nanomix, Inc. | Nano-electronic sensors for chemical and biological analytes, including capacitance and bio-membrane devices |
| US20030134433A1 (en) * | 2002-01-16 | 2003-07-17 | Nanomix, Inc. | Electronic sensing of chemical and biological agents using functionalized nanostructures |
| JP3710752B2 (en) * | 2002-01-29 | 2005-10-26 | 富士写真フイルム株式会社 | Method and apparatus for generating data for biochemical analysis |
| US10022078B2 (en) | 2004-07-13 | 2018-07-17 | Dexcom, Inc. | Analyte sensor |
| US8364229B2 (en) | 2003-07-25 | 2013-01-29 | Dexcom, Inc. | Analyte sensors having a signal-to-noise ratio substantially unaffected by non-constant noise |
| US7613491B2 (en) | 2002-05-22 | 2009-11-03 | Dexcom, Inc. | Silicone based membranes for use in implantable glucose sensors |
| DE10207616A1 (en) * | 2002-02-22 | 2003-09-04 | Sunyx Surface Nanotechnologies | Planar structure comprises ultraphobic surface and hydrophilic or oleophilic regions having an additional functionality, useful as a carrier for e.g. binding agents |
| US7504364B2 (en) * | 2002-03-01 | 2009-03-17 | Receptors Llc | Methods of making arrays and artificial receptors |
| AU2003220147A1 (en) * | 2002-03-11 | 2003-09-29 | Meso Scale Technologies, Llc. | System and method for flexibly representing and processing assay plates |
| US20030175818A1 (en) * | 2002-03-15 | 2003-09-18 | Ross Amelia A. | Devices and methods for isolating and recovering target cells |
| US7547931B2 (en) * | 2003-09-05 | 2009-06-16 | Nanomix, Inc. | Nanoelectronic capnometer adaptor including a nanoelectric sensor selectively sensitive to at least one gaseous constituent of exhaled breath |
| US7522040B2 (en) * | 2004-04-20 | 2009-04-21 | Nanomix, Inc. | Remotely communicating, battery-powered nanostructure sensor devices |
| US20070048181A1 (en) * | 2002-09-05 | 2007-03-01 | Chang Daniel M | Carbon dioxide nanosensor, and respiratory CO2 monitors |
| US7754155B2 (en) * | 2002-03-15 | 2010-07-13 | Ross Amelia A | Devices and methods for isolating target cells |
| US7714398B2 (en) * | 2002-09-05 | 2010-05-11 | Nanomix, Inc. | Nanoelectronic measurement system for physiologic gases and improved nanosensor for carbon dioxide |
| US20070048180A1 (en) * | 2002-09-05 | 2007-03-01 | Gabriel Jean-Christophe P | Nanoelectronic breath analyzer and asthma monitor |
| US7955483B2 (en) * | 2002-03-18 | 2011-06-07 | Honeywell International Inc. | Carbon nanotube-based glucose sensor |
| US20030194709A1 (en) * | 2002-04-10 | 2003-10-16 | Xing Yang | Hydrophobic zone device |
| US20040110277A1 (en) * | 2002-04-12 | 2004-06-10 | Seiko Epson Corporation | Sensor cell, bio-sensor, capacitance element manufacturing method, biological reaction detection method and genetic analytical method |
| US7611670B2 (en) * | 2002-04-23 | 2009-11-03 | Home Access Health Corporation | Quantitative analysis of a biological sample of unknown quantity |
| US7569184B2 (en) * | 2002-04-23 | 2009-08-04 | Home Access Health Corporation | Quantitative analysis of a biological sample of unknown quantity |
| ES2371539T3 (en) * | 2002-04-23 | 2012-01-04 | Home Access Health Corporation | QUANTITATIVE ANALYSIS OF A BIOLOGICAL SAMPLE OF UNKNOWN AMOUNT. |
| US20030202906A1 (en) * | 2002-04-24 | 2003-10-30 | Ching-Hsin Cho | Adding the amperage of the conductive film of the biosensor |
| US7112444B2 (en) * | 2002-04-24 | 2006-09-26 | Wisconsin Alumni Research Foundation | Method of performing gradient-based assays in a microfluidic device |
| US20040034177A1 (en) | 2002-05-02 | 2004-02-19 | Jian Chen | Polymer and method for using the polymer for solubilizing nanotubes |
| US6905667B1 (en) | 2002-05-02 | 2005-06-14 | Zyvex Corporation | Polymer and method for using the polymer for noncovalently functionalizing nanotubes |
| FR2839364B1 (en) * | 2002-05-03 | 2004-12-24 | Commissariat Energie Atomique | METHOD FOR DETECTING ELECTROCHIMILUMINESCENCE MOLECUKAR RECOGNITION |
| US20040014106A1 (en) * | 2002-05-14 | 2004-01-22 | Nanosphere, Inc. | Electrical detection of DNA hybridization and specific binding events |
| WO2003102216A2 (en) * | 2002-05-31 | 2003-12-11 | Diversa Corporation | Multiplexed systems for nucleic acid sequencing |
| SE0201874D0 (en) * | 2002-06-19 | 2002-06-19 | Biosensor Applications Sweden Ab Publ | Coated metal surface on solid support for displacement reactions |
| KR20050013592A (en) * | 2002-06-20 | 2005-02-04 | 아이젠인터내셔널인코포레이티드 | Electrochemiluminescence flow cell and flow cell components |
| US7948041B2 (en) | 2005-05-19 | 2011-05-24 | Nanomix, Inc. | Sensor having a thin-film inhibition layer |
| US20060003437A1 (en) * | 2002-06-26 | 2006-01-05 | Fujitsu Limited | Target detecting device and target capturer, device and method for molecular adsorption or desorption, and device and method for protein detection |
| DE10329928A1 (en) * | 2002-07-12 | 2004-02-05 | Benq Corp., Kweishan | Process for the preparation of biochips and biochip |
| US20040011650A1 (en) * | 2002-07-22 | 2004-01-22 | Frederic Zenhausern | Method and apparatus for manipulating polarizable analytes via dielectrophoresis |
| US7745203B2 (en) * | 2002-07-31 | 2010-06-29 | Kabushiki Kaisha Toshiba | Base sequence detection apparatus and base sequence automatic analyzing apparatus |
| WO2004015376A2 (en) * | 2002-08-07 | 2004-02-19 | National Institutes Of Health | Measurement systems and methods |
| WO2004018623A2 (en) * | 2002-08-16 | 2004-03-04 | Clinical Microarrays, Inc. | Substrates for isolating, reacting and microscopically analyzing materials |
| US20070114573A1 (en) * | 2002-09-04 | 2007-05-24 | Tzong-Ru Han | Sensor device with heated nanostructure |
| US20060263255A1 (en) * | 2002-09-04 | 2006-11-23 | Tzong-Ru Han | Nanoelectronic sensor system and hydrogen-sensitive functionalization |
| US20040137481A1 (en) * | 2002-09-16 | 2004-07-15 | Receptors Llc | Artificial receptor building blocks, components, and kits |
| WO2005003326A2 (en) | 2003-03-28 | 2005-01-13 | Receptors Llc. | Artificial receptors including reversibly immobilized building blocks and methods |
| US20050037381A1 (en) * | 2002-09-16 | 2005-02-17 | Receptors Llc | Artificial receptors, building blocks, and methods |
| US7469076B2 (en) * | 2003-09-03 | 2008-12-23 | Receptors Llc | Sensors employing combinatorial artificial receptors |
| US20050136483A1 (en) * | 2003-09-03 | 2005-06-23 | Receptors Llc | Nanodevices employing combinatorial artificial receptors |
| US20060057625A1 (en) * | 2002-09-16 | 2006-03-16 | Carlson Robert E | Scaffold-based artificial receptors and methods |
| US20050037429A1 (en) * | 2003-03-28 | 2005-02-17 | Receptors Llc | Artificial receptors including reversibly immobilized building blocks and methods |
| JPWO2004033614A1 (en) * | 2002-10-10 | 2006-02-09 | 富士通株式会社 | MOLECULE RELEASE DEVICE AND MOLECULE RELEASE METHOD |
| AU2002952384A0 (en) * | 2002-10-31 | 2002-11-14 | Swinburne University Of Technology | Structures |
| AU2003298655A1 (en) | 2002-11-15 | 2004-06-15 | Bioarray Solutions, Ltd. | Analysis, secure access to, and transmission of array images |
| AU2003295591A1 (en) * | 2002-11-15 | 2004-06-15 | The Regents Of The University Of California | System and method for multiplexed biomolecular analysis |
| CN1217192C (en) * | 2002-11-21 | 2005-08-31 | 北京博奥生物芯片有限责任公司 | Photoelectrochemical tag, method, instrument and kit for photoelectrochemical analysis |
| WO2004059280A2 (en) * | 2002-12-26 | 2004-07-15 | Meso Scale Technologies, Llc | Methods, compositions and kits for biomarker extraction |
| EP1583950B1 (en) * | 2002-12-26 | 2017-04-05 | Meso Scale Technologies, LLC. | Assay cartridges and methods of using the same |
| EP1578262A4 (en) | 2002-12-31 | 2007-12-05 | Therasense Inc | Continuous glucose monitoring system and methods of use |
| WO2005016115A2 (en) * | 2003-01-23 | 2005-02-24 | Montana State University | Biosensors utilizing dendrimer-immobilized ligands and their use thereof |
| US20060257638A1 (en) * | 2003-01-30 | 2006-11-16 | Glatkowski Paul J | Articles with dispersed conductive coatings |
| JP2004230690A (en) * | 2003-01-30 | 2004-08-19 | Takiron Co Ltd | Antistatic transparent resin sheet |
| EP1600498B1 (en) * | 2003-02-06 | 2012-08-15 | Cellseed Inc. | Substrates for high-density cell arrays and preparation processes and uses thereof |
| US7134999B2 (en) * | 2003-04-04 | 2006-11-14 | Dexcom, Inc. | Optimized sensor geometry for an implantable glucose sensor |
| WO2004097371A2 (en) * | 2003-04-25 | 2004-11-11 | Board Of Regents, The University Of Texas System | System and method for the detection of analytes |
| US9317922B2 (en) | 2003-05-16 | 2016-04-19 | Board Of Regents The University Of Texas System | Image and part recognition technology |
| WO2004104922A2 (en) * | 2003-05-16 | 2004-12-02 | Board Of Regents, The University Of Texas System | Image and part recognition technology |
| US6987400B2 (en) * | 2003-05-20 | 2006-01-17 | Panelvision Technologies | Testing flat panel display plates using high frequency AC signals |
| GB2421506B (en) | 2003-05-22 | 2008-07-09 | Zyvex Corp | Nanocomposites and methods thereto |
| US8066639B2 (en) | 2003-06-10 | 2011-11-29 | Abbott Diabetes Care Inc. | Glucose measuring device for use in personal area network |
| JP2005037140A (en) * | 2003-07-15 | 2005-02-10 | Fuji Photo Film Co Ltd | Assay method using biochemical analysis unit and device for cleaning the same |
| EP1649260A4 (en) * | 2003-07-25 | 2010-07-07 | Dexcom Inc | Electrode systems for electrochemical sensors |
| JP4708342B2 (en) | 2003-07-25 | 2011-06-22 | デックスコム・インコーポレーテッド | Oxygen augmentation membrane system for use in implantable devices |
| JP4038575B2 (en) * | 2003-07-25 | 2008-01-30 | 独立行政法人産業技術総合研究所 | Biosensor, biosensor device or biosensor storage method |
| US9763609B2 (en) | 2003-07-25 | 2017-09-19 | Dexcom, Inc. | Analyte sensors having a signal-to-noise ratio substantially unaffected by non-constant noise |
| US7774145B2 (en) | 2003-08-01 | 2010-08-10 | Dexcom, Inc. | Transcutaneous analyte sensor |
| US20100168657A1 (en) | 2003-08-01 | 2010-07-01 | Dexcom, Inc. | System and methods for processing analyte sensor data |
| US20190357827A1 (en) | 2003-08-01 | 2019-11-28 | Dexcom, Inc. | Analyte sensor |
| US7591801B2 (en) | 2004-02-26 | 2009-09-22 | Dexcom, Inc. | Integrated delivery device for continuous glucose sensor |
| US6931327B2 (en) | 2003-08-01 | 2005-08-16 | Dexcom, Inc. | System and methods for processing analyte sensor data |
| US9135402B2 (en) | 2007-12-17 | 2015-09-15 | Dexcom, Inc. | Systems and methods for processing sensor data |
| US8275437B2 (en) | 2003-08-01 | 2012-09-25 | Dexcom, Inc. | Transcutaneous analyte sensor |
| US8160669B2 (en) | 2003-08-01 | 2012-04-17 | Dexcom, Inc. | Transcutaneous analyte sensor |
| US7920906B2 (en) | 2005-03-10 | 2011-04-05 | Dexcom, Inc. | System and methods for processing analyte sensor data for sensor calibration |
| US20050064431A1 (en) * | 2003-09-09 | 2005-03-24 | Eastman Kodak Company | Biological microarray comprising polymer particles and method of use |
| EP1664724A4 (en) * | 2003-09-12 | 2007-05-02 | Nanomix Inc | Carbon dioxide nanoelectronic sensor |
| WO2005029705A2 (en) | 2003-09-18 | 2005-03-31 | Bioarray Solutions, Ltd. | Number coding for identification of subtypes of coded types of solid phase carriers |
| NZ546072A (en) | 2003-09-22 | 2009-08-28 | Bioarray Solutions Ltd | Surface immobilized polyelectrolyte with multiple functional groups capable of covalently bonding to biomolecules |
| GB0322443D0 (en) * | 2003-09-25 | 2003-10-29 | Rts Thurnall Plc | Compound storage vessel handling apparatus |
| US20050130226A1 (en) * | 2003-09-26 | 2005-06-16 | The University Of Cincinnati | Fully integrated protein lab-on-a-chip with smart microfluidics for spot array generation |
| JP4328168B2 (en) * | 2003-10-02 | 2009-09-09 | ソニー株式会社 | Detection unit for interaction between substances using capillary phenomenon, method using the detection unit, and substrate for bioassay |
| JP4617654B2 (en) * | 2003-10-06 | 2011-01-26 | ソニー株式会社 | Bioassay substrate manufacturing method using amphiphilic processing and bioassay substrate |
| WO2005042763A2 (en) | 2003-10-28 | 2005-05-12 | Bioarray Solutions Ltd. | Optimization of gene expression analysis using immobilized capture probes |
| EP1694859B1 (en) | 2003-10-29 | 2015-01-07 | Bioarray Solutions Ltd | Multiplexed nucleic acid analysis by fragmentation of double-stranded dna |
| US20050094807A1 (en) * | 2003-11-04 | 2005-05-05 | John Silzel | Accuracy array assay system and method |
| US9247900B2 (en) | 2004-07-13 | 2016-02-02 | Dexcom, Inc. | Analyte sensor |
| US11633133B2 (en) | 2003-12-05 | 2023-04-25 | Dexcom, Inc. | Dual electrode system for a continuous analyte sensor |
| EP2239566B1 (en) | 2003-12-05 | 2014-04-23 | DexCom, Inc. | Calibration techniques for a continuous analyte sensor |
| US8287453B2 (en) | 2003-12-05 | 2012-10-16 | Dexcom, Inc. | Analyte sensor |
| US8364231B2 (en) | 2006-10-04 | 2013-01-29 | Dexcom, Inc. | Analyte sensor |
| US8423114B2 (en) | 2006-10-04 | 2013-04-16 | Dexcom, Inc. | Dual electrode system for a continuous analyte sensor |
| US8774886B2 (en) | 2006-10-04 | 2014-07-08 | Dexcom, Inc. | Analyte sensor |
| JP4238716B2 (en) * | 2003-12-15 | 2009-03-18 | 富士ゼロックス株式会社 | Electrode for electrochemical measurement and manufacturing method thereof |
| JP4238715B2 (en) * | 2003-12-15 | 2009-03-18 | 富士ゼロックス株式会社 | Electrochemical measurement electrode |
| US7341834B2 (en) * | 2003-12-15 | 2008-03-11 | Geneohn Sciences, Inc. | Multiplexed electrochemical detection system and method |
| US7637868B2 (en) * | 2004-01-12 | 2009-12-29 | Dexcom, Inc. | Composite material for implantable device |
| DE102004005711A1 (en) * | 2004-02-05 | 2006-05-11 | Siemens Ag | Biosensor for the determination of an allergen with operating procedures |
| CA2556331A1 (en) | 2004-02-17 | 2005-09-29 | Therasense, Inc. | Method and system for providing data communication in continuous glucose monitoring and management system |
| US8808228B2 (en) | 2004-02-26 | 2014-08-19 | Dexcom, Inc. | Integrated medicament delivery device for use with continuous analyte sensor |
| IL160869A0 (en) * | 2004-03-15 | 2004-08-31 | Dnr Imaging System Ltd | Integrated system for real time gel electrophoresis running and documenting |
| US7048889B2 (en) * | 2004-03-23 | 2006-05-23 | Lucent Technologies Inc. | Dynamically controllable biological/chemical detectors having nanostructured surfaces |
| US20050221473A1 (en) * | 2004-03-30 | 2005-10-06 | Intel Corporation | Sensor array integrated circuits |
| JPWO2005104141A1 (en) * | 2004-04-20 | 2008-03-13 | タキロン株式会社 | Transparent conductive molded body for touch panel and touch panel |
| US8277713B2 (en) | 2004-05-03 | 2012-10-02 | Dexcom, Inc. | Implantable analyte sensor |
| US20050245799A1 (en) * | 2004-05-03 | 2005-11-03 | Dexcom, Inc. | Implantable analyte sensor |
| US8792955B2 (en) | 2004-05-03 | 2014-07-29 | Dexcom, Inc. | Transcutaneous analyte sensor |
| US20050266398A1 (en) * | 2004-06-01 | 2005-12-01 | Peter Lea | Method and device for rapid detection and quantitation of macro and micro matrices |
| US20060015020A1 (en) * | 2004-07-06 | 2006-01-19 | Dexcom, Inc. | Systems and methods for manufacture of an analyte-measuring device including a membrane system |
| US20060016700A1 (en) | 2004-07-13 | 2006-01-26 | Dexcom, Inc. | Transcutaneous analyte sensor |
| US7857760B2 (en) | 2004-07-13 | 2010-12-28 | Dexcom, Inc. | Analyte sensor |
| WO2006010143A2 (en) * | 2004-07-13 | 2006-01-26 | Neose Technologies, Inc. | Branched peg remodeling and glycosylation of glucagon-like peptide-1 [glp-1] |
| US7783333B2 (en) | 2004-07-13 | 2010-08-24 | Dexcom, Inc. | Transcutaneous medical device with variable stiffness |
| US8565848B2 (en) | 2004-07-13 | 2013-10-22 | Dexcom, Inc. | Transcutaneous analyte sensor |
| CA2475240A1 (en) * | 2004-07-20 | 2006-01-20 | Biophys, Inc. | Method and device to measure dynamic internal calibration true dose response curves |
| CA2475191A1 (en) * | 2004-07-20 | 2006-01-20 | Biophys, Inc. | System and method for rapid reading of macro and micro matrices |
| CA2475456A1 (en) | 2004-07-20 | 2006-01-20 | Biophys, Inc. | Method and device to optimize analyte and antibody substrate binding by least energy adsorption |
| WO2007013872A2 (en) * | 2004-07-22 | 2007-02-01 | The Board Of Trustees Of The University Of Illinois | Sensors employing single-walled carbon nanotubes |
| US7848889B2 (en) | 2004-08-02 | 2010-12-07 | Bioarray Solutions, Ltd. | Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification |
| US7296576B2 (en) | 2004-08-18 | 2007-11-20 | Zyvex Performance Materials, Llc | Polymers for enhanced solubility of nanomaterials, compositions and methods therefor |
| WO2006029234A1 (en) * | 2004-09-03 | 2006-03-16 | Receptors Llc | Combinatorial artificial receptors including tether building blocks |
| EP1789792A2 (en) * | 2004-09-11 | 2007-05-30 | Receptors LLC | Combinatorial artificial receptors including peptide building blocks |
| US7406391B2 (en) * | 2004-09-20 | 2008-07-29 | Affymetrix, Inc. | System, method, and computer product for detection instrument calibration |
| US20060240492A1 (en) * | 2004-11-12 | 2006-10-26 | Rusling James F | Carbon nanotube based immunosensors and methods of making and using |
| WO2007040559A2 (en) * | 2004-11-17 | 2007-04-12 | Bioveris | Electrochemiluminescent assay |
| US20060102471A1 (en) | 2004-11-18 | 2006-05-18 | Karl Maurer | Electrode array device having an adsorbed porous reaction layer |
| US20060138079A1 (en) * | 2004-12-27 | 2006-06-29 | Potyrailo Radislav A | Fabrication process of microfluidic devices |
| US20060166285A1 (en) * | 2005-01-26 | 2006-07-27 | Jainamma Krotz | Charged permeation layers for use on active electronic matrix devices |
| US7875426B2 (en) * | 2005-02-04 | 2011-01-25 | University Of South Florida | DNA biochip and methods of use |
| US8178046B2 (en) * | 2005-02-23 | 2012-05-15 | Sierra Sensors Gmbh | Microfluidic devices with SPR sensing capabilities |
| US8133178B2 (en) | 2006-02-22 | 2012-03-13 | Dexcom, Inc. | Analyte sensor |
| JP4435708B2 (en) * | 2005-03-22 | 2010-03-24 | 富士フイルム株式会社 | Biosensor |
| US20070034513A1 (en) | 2005-03-25 | 2007-02-15 | Combimatrix Corporation | Electrochemical deblocking solution for electrochemical oligomer synthesis on an electrode array |
| US8744546B2 (en) | 2005-05-05 | 2014-06-03 | Dexcom, Inc. | Cellulosic-based resistance domain for an analyte sensor |
| US9394167B2 (en) | 2005-04-15 | 2016-07-19 | Customarray, Inc. | Neutralization and containment of redox species produced by circumferential electrodes |
| US20060240312A1 (en) * | 2005-04-25 | 2006-10-26 | Tao Xie | Diffusion media, fuel cells, and fuel cell powered systems |
| US8486629B2 (en) | 2005-06-01 | 2013-07-16 | Bioarray Solutions, Ltd. | Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation |
| US8211279B2 (en) * | 2005-06-03 | 2012-07-03 | Board Of Regents Of The University Of Texas System | Electrochemistry and electrogenerated chemiluminescence with a single faradaic electrode |
| US8033501B2 (en) * | 2005-06-10 | 2011-10-11 | The Boeing Company | Method and apparatus for attaching electrically powered seat track cover to through hole seat track design |
| WO2007002580A2 (en) * | 2005-06-23 | 2007-01-04 | Bioveris Corporation | Diagnostic as say system with multi -well reagent container |
| AU2006292615A1 (en) * | 2005-09-16 | 2007-03-29 | Hyperion Catalysis International, Inc. | Conductive silicone and methods for preparing same |
| US20070065877A1 (en) | 2005-09-19 | 2007-03-22 | Combimatrix Corporation | Microarray having a base cleavable succinate linker |
| US20090054747A1 (en) * | 2005-10-31 | 2009-02-26 | Abbott Diabetes Care, Inc. | Method and system for providing analyte sensor tester isolation |
| US7927787B2 (en) | 2006-06-28 | 2011-04-19 | The Invention Science Fund I, Llc | Methods and systems for analysis of nutraceutical associated components |
| US20080241910A1 (en) | 2007-03-27 | 2008-10-02 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Devices for pathogen detection |
| US20080241909A1 (en) * | 2007-03-27 | 2008-10-02 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Microfluidic chips for pathogen detection |
| US7827042B2 (en) | 2005-11-30 | 2010-11-02 | The Invention Science Fund I, Inc | Methods and systems related to transmission of nutraceutical associated information |
| US20080179255A1 (en) * | 2007-01-29 | 2008-07-31 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Fluidic devices |
| US10296720B2 (en) | 2005-11-30 | 2019-05-21 | Gearbox Llc | Computational systems and methods related to nutraceuticals |
| US8000981B2 (en) | 2005-11-30 | 2011-08-16 | The Invention Science Fund I, Llc | Methods and systems related to receiving nutraceutical associated information |
| US8297028B2 (en) * | 2006-06-14 | 2012-10-30 | The Invention Science Fund I, Llc | Individualized pharmaceutical selection and packaging |
| US20080210748A1 (en) | 2005-11-30 | 2008-09-04 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware, | Systems and methods for receiving pathogen related information and responding |
| US20080178692A1 (en) * | 2007-01-29 | 2008-07-31 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Fluidic methods |
| US20080241000A1 (en) * | 2007-03-27 | 2008-10-02 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Systems for pathogen detection |
| US7974856B2 (en) | 2005-11-30 | 2011-07-05 | The Invention Science Fund I, Llc | Computational systems and methods related to nutraceuticals |
| US8340944B2 (en) | 2005-11-30 | 2012-12-25 | The Invention Science Fund I, Llc | Computational and/or control systems and methods related to nutraceutical agent selection and dosing |
| JP2009521684A (en) * | 2005-12-22 | 2009-06-04 | ハネウェル・インターナショナル・インコーポレーテッド | Portable sample analyzer cartridge |
| US8264137B2 (en) * | 2006-01-03 | 2012-09-11 | Samsung Electronics Co., Ltd. | Curing binder material for carbon nanotube electron emission cathodes |
| US9757061B2 (en) | 2006-01-17 | 2017-09-12 | Dexcom, Inc. | Low oxygen in vivo analyte sensor |
| WO2007089550A2 (en) | 2006-01-26 | 2007-08-09 | Nanoselect, Inc. | Cnt-based sensors: devices, processes and uses thereof |
| US20090278556A1 (en) * | 2006-01-26 | 2009-11-12 | Nanoselect, Inc. | Carbon Nanostructure Electrode Based Sensors: Devices, Processes and Uses Thereof |
| GB0601703D0 (en) * | 2006-01-27 | 2006-03-08 | Intellitect Water Ltd | Improvement To The Design And Construction Of Electrochemical Sensors |
| US8008067B2 (en) | 2006-02-13 | 2011-08-30 | University Of Maryland, Baltimore County | Microwave trigger metal-enhanced chemiluminescence (MT MEC) and spatial and temporal control of same |
| WO2007095527A2 (en) * | 2006-02-13 | 2007-08-23 | University Of Maryland Biotechnology Institute | Metal-enhanced chemiluminescence (mec) |
| US20070196863A1 (en) * | 2006-02-17 | 2007-08-23 | Hanson Technologies, Inc. | Prion protein detection |
| US20070292844A1 (en) * | 2006-03-22 | 2007-12-20 | Northrop Grumman Corporation | Enhanced biohazard detection system |
| JP4788436B2 (en) * | 2006-03-29 | 2011-10-05 | 日本電気株式会社 | Radio resource allocation method, radio resource allocation apparatus using the same, and base station |
| US20110160346A1 (en) * | 2006-03-31 | 2011-06-30 | Applied Nanotech Holdings, Inc. | Dispersion of carbon nanotubes by microfluidic process |
| US20070276077A1 (en) * | 2006-04-05 | 2007-11-29 | Nano-Proprietary, Inc. | Composites |
| US8129463B2 (en) * | 2006-03-31 | 2012-03-06 | Applied Nanotech Holdings, Inc. | Carbon nanotube-reinforced nanocomposites |
| US20080090951A1 (en) * | 2006-03-31 | 2008-04-17 | Nano-Proprietary, Inc. | Dispersion by Microfluidic Process |
| US8283403B2 (en) * | 2006-03-31 | 2012-10-09 | Applied Nanotech Holdings, Inc. | Carbon nanotube-reinforced nanocomposites |
| US8445587B2 (en) * | 2006-04-05 | 2013-05-21 | Applied Nanotech Holdings, Inc. | Method for making reinforced polymer matrix composites |
| WO2007143225A2 (en) | 2006-06-07 | 2007-12-13 | Abbott Diabetes Care, Inc. | Analyte monitoring system and method |
| GB0611701D0 (en) * | 2006-06-14 | 2006-07-26 | Biochrom Ltd | Analytical apparatus |
| US7687103B2 (en) * | 2006-08-31 | 2010-03-30 | Gamida For Life B.V. | Compositions and methods for preserving permeation layers for use on active electronic matrix devices |
| US7831287B2 (en) | 2006-10-04 | 2010-11-09 | Dexcom, Inc. | Dual electrode system for a continuous analyte sensor |
| EA201201363A1 (en) * | 2006-11-21 | 2013-08-30 | Медимейт Холдинг Б.В. | ION SENSOR FOR FLUID ENVIRONMENT AND METHOD FOR ITS MANUFACTURE |
| US8575663B2 (en) | 2006-11-22 | 2013-11-05 | President And Fellows Of Harvard College | High-sensitivity nanoscale wire sensors |
| CA2569971A1 (en) * | 2006-12-04 | 2008-06-04 | Umedik Inc. | Method for double-dip substrate spin optimization of coated micro array supports |
| EP1944078B1 (en) * | 2007-01-10 | 2011-03-02 | F. Hoffmann-La Roche AG | Device for determining an analyte in a liquid and method |
| US20080177166A1 (en) * | 2007-01-18 | 2008-07-24 | Provex Technologies, Llc | Ultrasensitive amperometric saliva glucose sensor strip |
| US20090050569A1 (en) * | 2007-01-29 | 2009-02-26 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Fluidic methods |
| US20080181821A1 (en) * | 2007-01-29 | 2008-07-31 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Microfluidic chips for allergen detection |
| US8617903B2 (en) | 2007-01-29 | 2013-12-31 | The Invention Science Fund I, Llc | Methods for allergen detection |
| US20080245740A1 (en) * | 2007-01-29 | 2008-10-09 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Fluidic methods |
| US10001496B2 (en) | 2007-01-29 | 2018-06-19 | Gearbox, Llc | Systems for allergen detection |
| US20080199894A1 (en) | 2007-02-15 | 2008-08-21 | Abbott Diabetes Care, Inc. | Device and method for automatic data acquisition and/or detection |
| US8123686B2 (en) | 2007-03-01 | 2012-02-28 | Abbott Diabetes Care Inc. | Method and apparatus for providing rolling data in communication systems |
| US20090227005A1 (en) * | 2007-03-27 | 2009-09-10 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Methods for pathogen detection |
| WO2008130895A2 (en) | 2007-04-14 | 2008-10-30 | Abbott Diabetes Care, Inc. | Method and apparatus for providing dynamic multi-stage signal amplification in a medical device |
| US7928850B2 (en) | 2007-05-08 | 2011-04-19 | Abbott Diabetes Care Inc. | Analyte monitoring system and methods |
| US8665091B2 (en) | 2007-05-08 | 2014-03-04 | Abbott Diabetes Care Inc. | Method and device for determining elapsed sensor life |
| US8461985B2 (en) | 2007-05-08 | 2013-06-11 | Abbott Diabetes Care Inc. | Analyte monitoring system and methods |
| US8456301B2 (en) | 2007-05-08 | 2013-06-04 | Abbott Diabetes Care Inc. | Analyte monitoring system and methods |
| CA2685361C (en) * | 2007-05-18 | 2014-05-06 | Medimate Holding B.V. | Test chip with plug for measuring the concentration of an analyte in a liquid, housing for test chip and socket for plug |
| US20200037875A1 (en) | 2007-05-18 | 2020-02-06 | Dexcom, Inc. | Analyte sensors having a signal-to-noise ratio substantially unaffected by non-constant noise |
| US20080292979A1 (en) * | 2007-05-22 | 2008-11-27 | Zhe Ding | Transparent conductive materials and coatings, methods of production and uses thereof |
| FR2916367B1 (en) * | 2007-05-25 | 2009-07-31 | Commissariat Energie Atomique | METHOD FOR MICROSYSTEM FIXATION OF PEPTIDE COMPOUND COMPOUNDS, SUCH AS PROTEINS, AND MICROSYSTEM INCORPORATING THESE COMPOUNDS |
| CA2688184A1 (en) | 2007-06-08 | 2008-12-18 | Dexcom, Inc. | Integrated medicament delivery device for use with continuous analyte sensor |
| EP2173467B1 (en) * | 2007-07-13 | 2016-05-04 | The Board Of Trustees Of The Leland Stanford Junior University | Method and apparatus using electric field for improved biological assays |
| US20090035707A1 (en) * | 2007-08-01 | 2009-02-05 | Yubing Wang | Rheology-controlled conductive materials, methods of production and uses thereof |
| WO2009021964A2 (en) * | 2007-08-13 | 2009-02-19 | Dublin City University | Optical biochip platform with plasmonic structure |
| US20090056589A1 (en) * | 2007-08-29 | 2009-03-05 | Honeywell International, Inc. | Transparent conductors having stretched transparent conductive coatings and methods for fabricating the same |
| US9452258B2 (en) | 2007-10-09 | 2016-09-27 | Dexcom, Inc. | Integrated insulin delivery system with continuous glucose sensor |
| US8417312B2 (en) | 2007-10-25 | 2013-04-09 | Dexcom, Inc. | Systems and methods for processing sensor data |
| US20090181359A1 (en) * | 2007-10-25 | 2009-07-16 | Lou Sheng C | Method of performing ultra-sensitive immunoassays |
| CN101842698B (en) * | 2007-10-31 | 2016-06-15 | 爱科来株式会社 | Analytical tool |
| US8222048B2 (en) * | 2007-11-05 | 2012-07-17 | Abbott Laboratories | Automated analyzer for clinical laboratory |
| WO2009061941A2 (en) | 2007-11-06 | 2009-05-14 | Osmetech Molecular Diagnostics | Baseless nucleotide analogues and uses thereof |
| US9839395B2 (en) | 2007-12-17 | 2017-12-12 | Dexcom, Inc. | Systems and methods for processing sensor data |
| US7727578B2 (en) | 2007-12-27 | 2010-06-01 | Honeywell International Inc. | Transparent conductors and methods for fabricating transparent conductors |
| US7960027B2 (en) * | 2008-01-28 | 2011-06-14 | Honeywell International Inc. | Transparent conductors and methods for fabricating transparent conductors |
| US7642463B2 (en) * | 2008-01-28 | 2010-01-05 | Honeywell International Inc. | Transparent conductors and methods for fabricating transparent conductors |
| CA2715628A1 (en) | 2008-02-21 | 2009-08-27 | Dexcom, Inc. | Systems and methods for processing, transmitting and displaying sensor data |
| US8583204B2 (en) | 2008-03-28 | 2013-11-12 | Dexcom, Inc. | Polymer membranes for continuous analyte sensors |
| US8682408B2 (en) | 2008-03-28 | 2014-03-25 | Dexcom, Inc. | Polymer membranes for continuous analyte sensors |
| US11730407B2 (en) | 2008-03-28 | 2023-08-22 | Dexcom, Inc. | Polymer membranes for continuous analyte sensors |
| WO2010011939A2 (en) * | 2008-07-25 | 2010-01-28 | Life Bioscience, Inc. | Assay plates, methods and systems having one or more etched features |
| US8560039B2 (en) | 2008-09-19 | 2013-10-15 | Dexcom, Inc. | Particle-containing membrane and particulate electrode for analyte sensors |
| JP5502413B2 (en) * | 2008-10-31 | 2014-05-28 | シスメックス株式会社 | Test chip, test substance detection device, and test substance specific detection method |
| US9801575B2 (en) | 2011-04-15 | 2017-10-31 | Dexcom, Inc. | Advanced analyte sensor calibration and error detection |
| US8374818B2 (en) * | 2008-12-19 | 2013-02-12 | Affymetrix, Inc. | System, method and apparatus for calibrating inspection tools |
| CA2750155A1 (en) | 2008-12-30 | 2010-07-08 | Centocor Ortho Biotech Inc. | Serum markers predicting clinical response to anti-tnf.alpha. antibodiesin patients with ankylosing spondylitis |
| GB2467338A (en) * | 2009-01-30 | 2010-08-04 | Sharp Kk | Electrical analyte sensor with optical output |
| JP6110067B2 (en) * | 2009-02-06 | 2017-04-05 | ノースウェスタン ユニバーシティ | Bursable liquid packaging and its use |
| JP2010237020A (en) * | 2009-03-31 | 2010-10-21 | Japan Aerospace Exploration Agency | Biomolecule detector and biomolecule detection method |
| DE102009015114B4 (en) | 2009-03-31 | 2014-05-22 | Siemens Aktiengesellschaft | Device in the manner of an electrochemical camera and method for producing and using the device |
| US20100261292A1 (en) | 2009-04-10 | 2010-10-14 | Meso Scale Technologies, Llc | Methods for Conducting Assays |
| CN113092562B (en) * | 2009-04-17 | 2024-03-29 | 环球生物医疗感测仪私人有限公司 | Electrochemical on-board control detection |
| US9226701B2 (en) | 2009-04-28 | 2016-01-05 | Abbott Diabetes Care Inc. | Error detection in critical repeating data in a wireless sensor system |
| US9767342B2 (en) | 2009-05-22 | 2017-09-19 | Affymetrix, Inc. | Methods and devices for reading microarrays |
| WO2010138856A1 (en) | 2009-05-29 | 2010-12-02 | Abbott Diabetes Care Inc. | Medical device antenna systems having external antenna configurations |
| US9131885B2 (en) | 2009-07-02 | 2015-09-15 | Dexcom, Inc. | Analyte sensors and methods of manufacturing same |
| CN106018857B (en) | 2009-07-27 | 2019-06-18 | 梅索磅秤技术有限公司 | Assay device, consumptive material and method |
| US8993346B2 (en) | 2009-08-07 | 2015-03-31 | Nanomix, Inc. | Magnetic carbon nanotube based biodetection |
| US9314195B2 (en) | 2009-08-31 | 2016-04-19 | Abbott Diabetes Care Inc. | Analyte signal processing device and methods |
| EP2473099A4 (en) | 2009-08-31 | 2015-01-14 | Abbott Diabetes Care Inc | Analyte monitoring system and methods for managing power and noise |
| WO2011038228A1 (en) | 2009-09-24 | 2011-03-31 | President And Fellows Of Harvard College | Bent nanowires and related probing of species |
| US20120028845A1 (en) * | 2009-10-04 | 2012-02-02 | Ross Teggatz | Sensor for Detecting Biological Agents in Fluid |
| WO2011090793A2 (en) | 2010-01-20 | 2011-07-28 | Customarray, Inc. | Multiplex microarray of serially deposited biomolecules on a microarray |
| WO2011133450A2 (en) * | 2010-04-19 | 2011-10-27 | Meso Scale Technologies, Llc. | Serology assays |
| KR101967487B1 (en) * | 2010-06-11 | 2019-04-09 | 랩마스터 오와이 | Low-cost electrode chip variants and methods for multi analyte analysis and ref-erencing based on cathodic electroluminescence |
| JP5938585B2 (en) * | 2010-10-07 | 2016-06-22 | パナソニックＩｐマネジメント株式会社 | Plasmon sensor |
| WO2012051519A2 (en) | 2010-10-14 | 2012-04-19 | The Johns Hopkins University | Biomarkers of brain injury |
| JP5943927B2 (en) | 2010-10-14 | 2016-07-05 | メソ スケール テクノロジーズ エルエルシー | Reagent storage in assay devices |
| ES2581553T3 (en) | 2010-10-25 | 2016-09-06 | F. Hoffmann-La Roche Ag | Use of signal enhancing compounds in electrochemiluminescent detection |
| US10648945B2 (en) | 2010-12-17 | 2020-05-12 | Malvern Panalytical Limited | Laser doppler electrophoresis using a diffusion barrier |
| US8702942B2 (en) * | 2010-12-17 | 2014-04-22 | Malvern Instruments, Ltd. | Laser doppler electrophoresis using a diffusion barrier |
| WO2012094459A2 (en) | 2011-01-06 | 2012-07-12 | Glezer Eli N | Assay cartridges and methods of using the same |
| JP2014518624A (en) | 2011-05-12 | 2014-08-07 | ザ・ジョンズ・ホプキンス・ユニバーシティー | Assay reagent for neurogranin diagnostic kit |
| US9593160B2 (en) | 2011-07-18 | 2017-03-14 | President And Fellows Of Harvard College | Engineered microbe-targeting molecules and uses thereof |
| JP2013088378A (en) * | 2011-10-21 | 2013-05-13 | Sony Corp | Chemical sensor, chemical sensor module, biomolecule detection device, and biomolecule detection method |
| ES2848204T3 (en) | 2011-10-26 | 2021-08-05 | Res Triangle Inst | Aerosol exposure monitoring |
| JP6300727B2 (en) | 2011-11-11 | 2018-03-28 | イーライ・エヌ・グレザー | Co-binder assisted assay |
| CN102495048A (en) * | 2011-12-09 | 2012-06-13 | 常熟理工学院 | Sandwich-type electrochemical luminescence detection cell |
| TWI638277B (en) * | 2012-03-05 | 2018-10-11 | Ｏｐｋｏ診斷法有限責任公司 | Assy system and method for determining a probability of an event associated with prostate cancer |
| GB201207869D0 (en) * | 2012-05-04 | 2012-06-20 | Accunostics Ltd | Pulsed signal testing of biological fluid |
| US9075042B2 (en) * | 2012-05-15 | 2015-07-07 | Wellstat Diagnostics, Llc | Diagnostic systems and cartridges |
| CN102749322A (en) * | 2012-07-04 | 2012-10-24 | 浙江大学 | Bipolar electrode electrochemiluminescent detection method for microfluidic droplet array |
| US20150184235A1 (en) * | 2012-07-10 | 2015-07-02 | Lexogen Gmbh | Flexible sensor carrier and method |
| US20150173883A1 (en) | 2012-07-18 | 2015-06-25 | President And Fellows Of Harvard College | Modification of surfaces for simultaneous repellency and targeted binding of desired moieties |
| EP2888349B1 (en) * | 2012-08-24 | 2022-02-09 | Yale University | System, device and method for high-throughput multi-plexed detection |
| US9968306B2 (en) | 2012-09-17 | 2018-05-15 | Abbott Diabetes Care Inc. | Methods and apparatuses for providing adverse condition notification with enhanced wireless communication range in analyte monitoring systems |
| EP2903739A2 (en) * | 2012-10-01 | 2015-08-12 | University Of Oslo | Micro-scale liquid-liquid-liquid extraction |
| CN103018441A (en) * | 2012-12-31 | 2013-04-03 | 西南大学 | Multicomponent immunoassay method based on time-resolved chemiluminescence |
| CA3071710C (en) | 2013-01-04 | 2021-03-16 | Meso Scale Technologies, Llc. | Assay apparatuses, methods and reagents |
| EP2972353B1 (en) | 2013-03-11 | 2023-02-22 | Meso Scale Technologies, LLC | Improved methods for conducting multiplexed assays |
| US10114015B2 (en) | 2013-03-13 | 2018-10-30 | Meso Scale Technologies, Llc. | Assay methods |
| CA2904181A1 (en) | 2013-03-13 | 2014-10-09 | Anahit Aghvanyan | Sandwich immunoassay comprising anchoring reagent |
| US9416776B2 (en) * | 2013-03-15 | 2016-08-16 | Siemens Healthcare Diagnostics Inc. | Microfluidic distributing device |
| CN105026932B (en) | 2013-03-15 | 2017-06-13 | 西门子医疗保健诊断公司 | Micro-fluidic distributing equipment |
| CN105209532B (en) | 2013-03-15 | 2017-11-28 | 路博润先进材料公司 | CPVC compositions without heavy metal |
| JP6553020B2 (en) | 2013-03-15 | 2019-07-31 | ジーピービー デット ホールディングス トゥー エルエルシー | Automated immunoassay system for performing diagnostic analysis on allergies and autoimmune diseases |
| WO2014200767A1 (en) | 2013-06-12 | 2014-12-18 | The General Hospital Corporation | Methods, kits, and systems for multiplexed detection of target molecules and uses thereof |
| US10534003B2 (en) | 2013-07-17 | 2020-01-14 | The Johns Hopkins University | Multi-protein biomarker assay for brain injury detection and outcome |
| CN103499571B (en) * | 2013-10-14 | 2016-03-23 | 南京大学 | A kind of electrochemiluminescdetection detection device |
| US10513546B2 (en) | 2013-12-18 | 2019-12-24 | President And Fellows Of Harvard College | CRP capture/detection of gram positive bacteria |
| KR101940981B1 (en) | 2014-05-05 | 2019-01-23 | 3디 글래스 솔루션즈 인코포레이티드 | 2d and 3d inductors antenna and transformers fabricating photoactive substrates |
| CA2947747C (en) | 2014-05-09 | 2024-03-05 | Meso Scale Technologies, Llc. | Graphene-modified electrodes |
| KR20230022268A (en) | 2014-05-15 | 2023-02-14 | 메소 스케일 테크놀러지즈, 엘엘시 | Improved assay methods |
| WO2016044697A1 (en) | 2014-09-19 | 2016-03-24 | The Johns Hopkins University | Biomarkers of cognitive dysfunction |
| CN112782140A (en) | 2014-12-03 | 2021-05-11 | 伊索普莱西斯公司 | Analysis and screening of cellular secretion characteristics |
| CN107257711B (en) | 2014-12-05 | 2019-11-22 | 加利福尼亚大学董事会 | The machine glazing for reticulating ground wire with collection activates microfluidic device |
| US9658190B2 (en) * | 2014-12-18 | 2017-05-23 | Genia Technologies, Inc. | Printed electrode |
| US11353448B2 (en) | 2015-02-13 | 2022-06-07 | California Institute Of Technology | Methods and compositions for quantifying metabolites and proteins from single cells |
| WO2016134365A1 (en) | 2015-02-20 | 2016-08-25 | The Johns Hopkins University | Biomarkers of myocardial injury |
| US10294451B2 (en) | 2015-04-22 | 2019-05-21 | University Of Maryland, Baltimore County | Flow and static lysing systems and methods for ultra-rapid isolation and fragmentation of biological materials by microwave irradiation |
| WO2017015531A1 (en) | 2015-07-22 | 2017-01-26 | University Of Maryland, Baltimore County | Hydrophilic coatings of plasmonic metals to enable low volume metal-enhanced fluorescence |
| US10435457B2 (en) | 2015-08-06 | 2019-10-08 | President And Fellows Of Harvard College | Microbe-binding molecules and uses thereof |
| US10070533B2 (en) | 2015-09-30 | 2018-09-04 | 3D Glass Solutions, Inc. | Photo-definable glass with integrated electronics and ground plane |
| US11264167B2 (en) | 2016-02-25 | 2022-03-01 | 3D Glass Solutions, Inc. | 3D capacitor and capacitor array fabricating photoactive substrates |
| WO2017153574A1 (en) | 2016-03-11 | 2017-09-14 | Roche Diagnostics Gmbh | Branched-chain amines in electrochemiluminescence detection |
| CN105758849B (en) * | 2016-03-16 | 2018-03-27 | 济南大学 | A kind of preparation method and application of the difunctional stilbestrol without enzyme sensor based on Two-dimensional Composites structure |
| WO2017177171A1 (en) | 2016-04-08 | 2017-10-12 | 3D Glass Solutions, Inc. | Methods of fabricating photosensitive substrates suitable for optical coupler |
| RU2731841C2 (en) * | 2016-04-22 | 2020-09-08 | Иллюмина, Инк. | Device and method based on photon structures for use in obtaining luminescent images of sites within pixel |
| DK3538891T3 (en) | 2016-11-11 | 2022-03-28 | Isoplexis Corp | COMPOSITIONS AND PROCEDURES FOR CONTEMPORARY GENOMIC, TRANSCRIPTOMIC AND PROTEOMIC ANALYSIS OF SINGLE CELLS |
| CN110226084A (en) | 2016-11-22 | 2019-09-10 | 伊索普莱克西斯公司 | For the systems, devices and methods of cell capture and its manufacturing method |
| CN106645109B (en) * | 2016-12-29 | 2019-11-26 | Tcl集团股份有限公司 | A kind of method, apparatus and system detecting hormone |
| CN107245755B (en) * | 2017-04-21 | 2019-05-21 | 深圳技术大学 | Light assisted electrochemical etching device suitable for the synchronous experiment of Multi-example |
| KR102273624B1 (en) | 2017-04-28 | 2021-07-07 | 3디 글래스 솔루션즈 인코포레이티드 | Rf Circulator |
| CN107515210B (en) * | 2017-06-12 | 2020-04-14 | 南京大学 | Handheld high-flux matrix electrochemiluminescence chip analysis system |
| JP6995891B2 (en) | 2017-07-07 | 2022-01-17 | スリーディー グラス ソリューションズ，インク | 2D and 3D RF centralized device for RF systems in packaged photoactive glass substrates |
| TWI758537B (en) * | 2017-09-01 | 2022-03-21 | 大陸商深圳華大智造科技有限公司 | An injection molded microfluidic/fluidic cartridge integrated with silicon-based sensor |
| US11331022B2 (en) | 2017-10-24 | 2022-05-17 | Dexcom, Inc. | Pre-connected analyte sensors |
| US20190120785A1 (en) | 2017-10-24 | 2019-04-25 | Dexcom, Inc. | Pre-connected analyte sensors |
| EP3724946B1 (en) | 2017-12-15 | 2024-04-17 | 3D Glass Solutions, Inc. | Coupled transmission line resonate rf filter |
| AU2018399638B2 (en) | 2018-01-04 | 2021-09-02 | 3D Glass Solutions, Inc. | Impedance matching conductive structure for high efficiency RF circuits |
| EP4310184A3 (en) | 2018-02-23 | 2024-05-01 | Meso Scale Technologies, LLC. | Methods of screening antigen-binding molecules by normalizing for the concentration of antigen-binding molecule |
| TWI668440B (en) * | 2018-03-23 | 2019-08-11 | 台灣奈米碳素股份有限公司 | Electrode assembly for Quality Control of Electrochemical Impedance Biochips and Method for Quality Control of Electrochemical Impedance Biochips |
| KR102626372B1 (en) | 2018-04-10 | 2024-01-16 | 3디 글래스 솔루션즈 인코포레이티드 | Rf integrated power condition capacitor |
| WO2019236209A2 (en) * | 2018-04-27 | 2019-12-12 | Jin Byung Ju | Methods for high-content drug screening |
| US10903545B2 (en) | 2018-05-29 | 2021-01-26 | 3D Glass Solutions, Inc. | Method of making a mechanically stabilized radio frequency transmission line device |
| US20190369119A1 (en) | 2018-06-04 | 2019-12-05 | Avon Products, Inc. | Protein Biomarkers for Identifying and Treating Aging Skin and Skin Conditions |
| WO2020031185A2 (en) * | 2018-08-06 | 2020-02-13 | Carmel Diagnostics Ltd. | Cellular tissue analysis method and device |
| KR102518025B1 (en) | 2018-09-17 | 2023-04-06 | 3디 글래스 솔루션즈 인코포레이티드 | High efficiency compact slotted antenna with a ground plane |
| MX2020014066A (en) | 2018-12-17 | 2021-05-27 | Illumina Inc | Flow cells and sequencing kits. |
| WO2020139951A1 (en) | 2018-12-28 | 2020-07-02 | 3D Glass Solutions, Inc. | Heterogenous integration for rf, microwave and mm wave systems in photoactive glass substrates |
| KR102392858B1 (en) | 2018-12-28 | 2022-05-03 | 3디 글래스 솔루션즈 인코포레이티드 | Toroidal Capacitor RF, Microwave, and Mm Wave Systems |
| KR20210146884A (en) | 2019-01-03 | 2021-12-06 | 메소 스케일 테크놀러지즈, 엘엘시 | Compositions and methods for performing assay measurements |
| CN109725025B (en) * | 2019-02-22 | 2020-12-08 | 清华大学 | Testing equipment and method for membrane electrode of solid oxide fuel cell |
| EP3931201A1 (en) | 2019-03-01 | 2022-01-05 | Meso Scale Technologies, LLC | Electrochemiluminescent labeled probes for use in immunoassay methods, methods using such and kits comprising same |
| US20200300801A1 (en) * | 2019-03-21 | 2020-09-24 | The United States Of America, As Represented By The Secretary Of Agriculture | Flow-Through Electrochemical Detection System |
| KR20210147040A (en) | 2019-04-05 | 2021-12-06 | 3디 글래스 솔루션즈 인코포레이티드 | Glass-Based Blank Substrate Integrated Waveguide Device |
| CA3136642C (en) | 2019-04-18 | 2023-01-03 | 3D Glass Solutions, Inc. | High efficiency die dicing and release |
| CN113939599A (en) | 2019-06-07 | 2022-01-14 | 豪夫迈·罗氏有限公司 | Hybrid all-LNA oligonucleotides |
| CN110412021B (en) * | 2019-08-06 | 2022-04-29 | 华南师范大学 | Electrochemical luminescence micro-fluidic chip sharing bipolar electrode cathode and application thereof |
| EP4121988A4 (en) | 2020-04-17 | 2023-08-30 | 3D Glass Solutions, Inc. | Broadband induction |
| WO2021236584A1 (en) | 2020-05-19 | 2021-11-25 | Meso Scale Technologies, Llc. | Methods, compositions, and kits for nucleic acid detection |
| US20220018785A1 (en) | 2020-07-01 | 2022-01-20 | Meso Scale Technologies, Llc. | Compositions and methods for assay measurements |
| WO2022040529A2 (en) | 2020-08-21 | 2022-02-24 | Meso Scale Technologies, Llc. | Auxiliary electrodes and methods for using and manufacturing the same |
| CN112159653B (en) * | 2020-09-29 | 2023-10-03 | 东北师范大学 | Electrochemiluminescence reagent and application thereof |
| EP4244512A4 (en) | 2020-11-16 | 2024-01-17 | Siemens Healthcare Diagnostics Inc | Valve for microfluidic device |
| CN116635083A (en) | 2020-12-22 | 2023-08-22 | 豪夫迈·罗氏有限公司 | Method for detecting target analytes in a sample |
| CN113144917B (en) * | 2021-04-23 | 2022-07-22 | 西南石油大学 | Sea urchin-shaped nickel-cobalt hydrotalcite stainless steel net film and preparation method and application thereof |
| US11513097B1 (en) | 2021-05-21 | 2022-11-29 | PERSOWN, Inc. | Methods of obtaining and using electrochemical diagnostic results |
| IL310671A (en) | 2021-08-13 | 2024-04-01 | Meso Scale Technologies Llc | Electrochemical cell devices and methods of manufacturing |
| WO2023129884A1 (en) | 2021-12-30 | 2023-07-06 | Meso Scale Technologies, Llc. | Methods for electrochemiluminescence detection |
| WO2023133377A1 (en) | 2022-01-04 | 2023-07-13 | Meso Scale Technologies, Llc. | Electrochemical cell devices and methods of manufacturing |
| WO2023156510A1 (en) | 2022-02-18 | 2023-08-24 | F. Hoffmann-La Roche Ag | Method for detecting an analyte of interest in a sample |
Family Cites Families (53)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4280815A (en) * | 1979-06-18 | 1981-07-28 | Technicon Instruments Corporation | Electrochemiluminescent immunoassay and apparatus therefor |
| US4826759A (en) * | 1984-10-04 | 1989-05-02 | Bio-Metric Systems, Inc. | Field assay for ligands |
| US5310687A (en) * | 1984-10-31 | 1994-05-10 | Igen, Inc. | Luminescent metal chelate labels and means for detection |
| US5238808A (en) | 1984-10-31 | 1993-08-24 | Igen, Inc. | Luminescent metal chelate labels and means for detection |
| US5221605A (en) | 1984-10-31 | 1993-06-22 | Igen, Inc. | Luminescent metal chelate labels and means for detection |
| US5171560A (en) * | 1984-12-06 | 1992-12-15 | Hyperion Catalysis International | Carbon fibrils, method for producing same, and encapsulated catalyst |
| US4663230A (en) * | 1984-12-06 | 1987-05-05 | Hyperion Catalysis International, Inc. | Carbon fibrils, method for producing same and compositions containing same |
| US5165909A (en) | 1984-12-06 | 1992-11-24 | Hyperion Catalysis Int'l., Inc. | Carbon fibrils and method for producing same |
| US4652333A (en) * | 1985-06-19 | 1987-03-24 | Honeywell Inc. | Etch process monitors for buried heterostructures |
| US5468606A (en) * | 1989-09-18 | 1995-11-21 | Biostar, Inc. | Devices for detection of an analyte based upon light interference |
| KR960016340B1 (en) * | 1986-04-30 | 1996-12-09 | 이겐 인코오포레이팃드 | Electrochemiluminescent assays |
| US5147806A (en) * | 1988-04-29 | 1992-09-15 | Igen, Inc. | Method and apparatus for conducting electrochemiluminescence measurements |
| US5591581A (en) * | 1986-04-30 | 1997-01-07 | Igen, Inc. | Electrochemiluminescent rhenium moieties and methods for their use |
| US4891321A (en) * | 1987-10-21 | 1990-01-02 | Hubscher Thomas T | Apparatus for performing determinations of immune reactants in biological fluids |
| ZA889546B (en) | 1987-12-24 | 1990-08-29 | Igen Inc | Chemical sensors employing catalytic antibodies |
| US5308754A (en) * | 1988-03-21 | 1994-05-03 | Kankare Jouko J | Electrogenerated luminescence in solution |
| US5093268A (en) | 1988-04-28 | 1992-03-03 | Igen, Inc. | Apparatus for conducting a plurality of simultaneous measurements of electrochemiluminescent phenomena |
| CA1325527C (en) * | 1988-04-28 | 1993-12-28 | Nicholas Leventis | Apparatus for conducting a plurality of simultaneous measurements of electrochemiluminescent phenomena |
| US5061445A (en) * | 1988-11-03 | 1991-10-29 | Igen, Inc. | Apparatus for conducting measurements of electrochemiluminescent phenomena |
| EP0757252A3 (en) * | 1988-11-03 | 1997-04-16 | Igen Inc | Electrochemiluminescent assays |
| US5068088A (en) | 1988-11-03 | 1991-11-26 | Igen, Inc. | Method and apparatus for conducting electrochemiluminescent measurements |
| US5247243A (en) | 1988-11-03 | 1993-09-21 | Igen, Inc. | Method and apparatus for conducting electrochemiluminescent measurements |
| US5296191A (en) | 1988-11-03 | 1994-03-22 | Igen, Inc. | Method and apparatus for conducting electrochemiluminescent measurements |
| SE8804074D0 (en) * | 1988-11-10 | 1988-11-10 | Pharmacia Ab | SENSOR UNIT AND ITS USE IN BIOSENSOR SYSTEM |
| JP2863192B2 (en) * | 1989-04-19 | 1999-03-03 | ハイピリオン・カタリシス・インターナシヨナル・インコーポレイテツド | Thermoplastic elastomer composition |
| KR940000623B1 (en) * | 1989-05-15 | 1994-01-26 | 히페리온 카탈리시스 인터내셔날 | Surface treatment of carbon microfibers |
| JP3029115B2 (en) | 1989-07-21 | 2000-04-04 | ハイピリオン・カタリシス・インターナショナル・インコーポレイテッド | Conductive sheet |
| US5098771A (en) * | 1989-07-27 | 1992-03-24 | Hyperion Catalysis International | Conductive coatings and inks |
| US5110693A (en) * | 1989-09-28 | 1992-05-05 | Hyperion Catalysis International | Electrochemical cell |
| US5324457A (en) * | 1989-10-02 | 1994-06-28 | Board Of Regents, The University Of Tx System | Devices and methods for generating electrogenerated chemiluminescence |
| JPH03122544A (en) | 1989-10-06 | 1991-05-24 | Hitachi Ltd | Measuring apparatus of mechanical property of thin film material |
| JP2869866B2 (en) * | 1989-10-06 | 1999-03-10 | ティーディーケイ株式会社 | Container for electrochemiluminescence detection |
| US5189549A (en) | 1990-02-26 | 1993-02-23 | Molecular Displays, Inc. | Electrochromic, electroluminescent and electrochemiluminescent displays |
| GB2244135B (en) * | 1990-05-04 | 1994-07-13 | Gen Electric Co Plc | Sensor devices |
| EP0478319B1 (en) * | 1990-09-28 | 1997-04-02 | Kabushiki Kaisha Toshiba | Gene detection method |
| JP2573443B2 (en) * | 1990-09-28 | 1997-01-22 | 株式会社東芝 | Gene detection method |
| US5776672A (en) * | 1990-09-28 | 1998-07-07 | Kabushiki Kaisha Toshiba | Gene detection method |
| IL100867A (en) * | 1991-02-06 | 1995-12-08 | Igen Inc | Method and apparatus for improved luminescence assays |
| JPH0534345A (en) * | 1991-02-19 | 1993-02-09 | Tdk Corp | Measuring method of antigen-antibody utilizing chemiluminescence |
| JPH04297430A (en) * | 1991-03-15 | 1992-10-21 | Idemitsu Kosan Co Ltd | Polyphenyl derivative and production thereof |
| ATE219244T1 (en) * | 1991-03-27 | 2002-06-15 | Ambri Ltd | IONIC RESERVOIR ON THE SURFACE OF AN ELECTRODE |
| EP0532762B1 (en) * | 1991-03-28 | 1998-09-09 | Nippon Shoji Kabushiki Kaisha | Simple analyzing device |
| US5340716A (en) * | 1991-06-20 | 1994-08-23 | Snytex (U.S.A.) Inc. | Assay method utilizing photoactivated chemiluminescent label |
| EP0522677B1 (en) * | 1991-07-10 | 1996-12-18 | TDK Corporation | Method of measuring concentration of immunoreactant using electrochemiluminescence |
| US5632957A (en) * | 1993-11-01 | 1997-05-27 | Nanogen | Molecular biological diagnostic systems including electrodes |
| CA2124087C (en) * | 1991-11-22 | 2002-10-01 | James L. Winkler | Combinatorial strategies for polymer synthesis |
| DE4216980C2 (en) * | 1992-05-19 | 1994-04-21 | Eppendorf Geraetebau Netheler | Immunosensory detection method |
| JPH0618472A (en) * | 1992-06-30 | 1994-01-25 | New Oji Paper Co Ltd | Electrode for electrochemical measurement |
| JP3706636B2 (en) * | 1992-11-27 | 2005-10-12 | キヤノン株式会社 | Nucleic acid hybrid detection method, probe, target nucleic acid confirmation method, and mismatch detection method in double-stranded nucleic acid hybrid |
| US5466416A (en) * | 1993-05-14 | 1995-11-14 | Ghaed; Ali | Apparatus and methods for carrying out electrochemiluminescence test measurements |
| EP0777741A4 (en) * | 1994-08-26 | 1999-01-13 | Igen Inc | Biosensor for and method of electrogenerated chemiluminescent detection of nucleic acid adsorbed to a solid surface |
| US6852502B1 (en) * | 1995-06-06 | 2005-02-08 | Bioveris Corporation | Electrochemiluminescent enzyme biosensors |
| CN1661115A (en) * | 1995-03-10 | 2005-08-31 | 梅索磅秤技术有限公司 | Multi-array, multi-specific electrochemiluminescence testing |
-
1996
- 1996-03-06 CN CN2005100057208A patent/CN1661115A/en active Pending
- 1996-03-06 KR KR1019970706257A patent/KR100449615B1/en not_active IP Right Cessation
- 1996-03-06 BR BRPI9607193-1A patent/BR9607193B1/en not_active IP Right Cessation
- 1996-03-06 CA CA2704228A patent/CA2704228C/en not_active Expired - Lifetime
- 1996-03-06 CA CA2213854A patent/CA2213854C/en not_active Expired - Lifetime
- 1996-03-06 CN CNB961938404A patent/CN1192097C/en not_active Expired - Lifetime
- 1996-03-06 EP EP10178531.9A patent/EP2280268B1/en not_active Expired - Lifetime
- 1996-03-06 HU HU9801679A patent/HUP9801679A3/en not_active Application Discontinuation
- 1996-03-06 AU AU54205/96A patent/AU720625B2/en not_active Expired
- 1996-03-06 TW TW085102864A patent/TW555852B/en not_active IP Right Cessation
- 1996-03-06 US US08/611,804 patent/US6066448A/en not_active Expired - Lifetime
- 1996-03-06 JP JP8527737A patent/JPH11502617A/en not_active Withdrawn
- 1996-03-06 NZ NZ306051A patent/NZ306051A/en not_active IP Right Cessation
- 1996-03-06 EP EP96911269.7A patent/EP0821726B1/en not_active Expired - Lifetime
- 1996-03-06 CZ CZ0284497A patent/CZ299135B6/en not_active IP Right Cessation
- 1996-03-06 WO PCT/US1996/003190 patent/WO1996028538A1/en active IP Right Grant
- 1996-03-06 EA EA199700219A patent/EA001198B1/en not_active IP Right Cessation
-
1997
- 1997-03-06 US US08/814,141 patent/US6090545A/en not_active Expired - Lifetime
-
2005
- 2005-10-11 JP JP2005296368A patent/JP4365362B2/en not_active Expired - Lifetime
-
2007
- 2007-10-22 JP JP2007273467A patent/JP4365434B2/en not_active Expired - Lifetime
-
2009
- 2009-03-02 JP JP2009047842A patent/JP4754003B2/en not_active Expired - Lifetime
-
2011
- 2011-06-03 HK HK11105611.5A patent/HK1151588A1/en not_active IP Right Cessation
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2213854A1 (en) | Multi-array, multi-specific electrochemiluminescence testing | |
| AU624621B2 (en) | Method and device employing photoresponsive semiconductor electrode for determining analyte | |
| Tang et al. | Electrochemical immuno-bioanalysis for carcinoma antigen 125 based on thionine and gold nanoparticles-modified carbon paste interface | |
| US6602400B1 (en) | Method for enhanced bio-conjugation events | |
| CA1223923A (en) | Photoresponsive redox detection and discrimination | |
| JP3764779B2 (en) | Analysis method using convex regions | |
| EP0402917B1 (en) | Biosensors employing electrical, optical and mechanical signals | |
| KR0144002B1 (en) | Chemical sensors employing catalytic antibodies | |
| Brewster et al. | Immunoelectrochemical assays for bacteria: use of epifluorescence microscopy and rapid-scan electrochemical techniques in development of an assay for Salmonella | |
| Dong et al. | Surface plasmon resonance sensor for profenofos detection using molecularly imprinted thin film as recognition element | |
| US5149629A (en) | Coulometric assay system | |
| US20030119208A1 (en) | Electrochemical immunosensor and kit and method for detecting biochemical anylyte using the sensor | |
| EP1371974A1 (en) | Apparatus for detecting interaction between biopolymer and ligand and method thereof | |
| US20140274760A1 (en) | Smart glass slide for microarrays | |
| US8029985B2 (en) | Amplified bioassay | |
| JPH05264552A (en) | Method and apparatus for specific bond analysis | |
| WO2000052456A1 (en) | Biosensor device and method | |
| US4356260A (en) | Enzymatic indicator system | |
| US6458600B1 (en) | Method for producing laterally organized structures on supporting surfaces | |
| JP2010515910A (en) | How to detect an analyte | |
| US7402381B2 (en) | Method of immobilizing molecules onto a solid phase substrate and method of fabricating a biosensor using the method | |
| EP2657347A1 (en) | Electrochemical competitive assay and use thereof | |
| US6649361B1 (en) | Surface plasmon resonance enzyme sensor | |
| JPH04118554A (en) | Electrochemical method for measuring enzyme and bio-sensor | |
| WO2008007499A1 (en) | Electrochemical immunoassay chip |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |
Effective date: 20160307 |