CA2214395A1 - Proteinase k variants having decreased adsorption and increased hydrolysis - Google Patents
Proteinase k variants having decreased adsorption and increased hydrolysisInfo
- Publication number
- CA2214395A1 CA2214395A1 CA002214395A CA2214395A CA2214395A1 CA 2214395 A1 CA2214395 A1 CA 2214395A1 CA 002214395 A CA002214395 A CA 002214395A CA 2214395 A CA2214395 A CA 2214395A CA 2214395 A1 CA2214395 A1 CA 2214395A1
- Authority
- CA
- Canada
- Prior art keywords
- proteinase
- variants
- loop region
- wild
- acid sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D17/00—Detergent materials or soaps characterised by their shape or physical properties
- C11D17/0047—Detergents in the form of bars or tablets
- C11D17/0065—Solid detergents containing builders
- C11D17/0073—Tablets
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D17/00—Detergent materials or soaps characterised by their shape or physical properties
- C11D17/0047—Detergents in the form of bars or tablets
- C11D17/0065—Solid detergents containing builders
- C11D17/0069—Laundry bars
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D17/00—Detergent materials or soaps characterised by their shape or physical properties
- C11D17/0047—Detergents in the form of bars or tablets
- C11D17/0065—Solid detergents containing builders
- C11D17/0073—Tablets
- C11D17/0078—Multilayered tablets
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/0005—Other compounding ingredients characterised by their effect
- C11D3/0052—Gas evolving or heat producing compositions
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/0005—Other compounding ingredients characterised by their effect
- C11D3/0078—Compositions for cleaning contact lenses, spectacles or lenses
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
Abstract
The present invention relates to proteinase K variants having a modified amino acid sequence of wild-type proteinase K amino acid sequence, the wild-type amino acid sequence comprising a first loop region, a second loop region, a third loop region, a fourth loop region, a fifth loop region and a sixth loop region; wherein the modified amino acid sequence comprises different amino acids than that occurring in wild-type proteinase K (i.e., substitution) at specifically identified positions in one or more of the loop regions whereby the proteinase K variant has decreased adsorption to, and increased hydrolysis of, an insoluble substrate as compared to the wild-type proteinase K. The present invention also relates to DNA sequences encoding such proteinase K
variants. The present invention also relates to compositions comprising such proteinase K variants for cleaning a variety of surfaces.
variants. The present invention also relates to compositions comprising such proteinase K variants for cleaning a variety of surfaces.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US40157495A | 1995-03-09 | 1995-03-09 | |
US401,574 | 1995-03-09 | ||
PCT/US1996/003005 WO1996028556A2 (en) | 1995-03-09 | 1996-03-06 | Proteinase k variants having decreased adsorption and increased hydrolysis |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2214395A1 true CA2214395A1 (en) | 1996-09-19 |
CA2214395C CA2214395C (en) | 2001-10-30 |
Family
ID=23588288
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002214395A Expired - Fee Related CA2214395C (en) | 1995-03-09 | 1996-03-06 | Proteinase k variants having decreased adsorption and increased hydrolysis |
Country Status (14)
Country | Link |
---|---|
US (1) | US6451574B1 (en) |
EP (1) | EP0813601A2 (en) |
JP (1) | JPH11501814A (en) |
CN (1) | CN1183809A (en) |
AU (1) | AU5092796A (en) |
BR (1) | BR9607677A (en) |
CA (1) | CA2214395C (en) |
CZ (1) | CZ279997A3 (en) |
HU (1) | HUP9801638A3 (en) |
IL (1) | IL117350A0 (en) |
MA (1) | MA23820A1 (en) |
MX (1) | MX9706841A (en) |
WO (1) | WO1996028556A2 (en) |
ZA (1) | ZA961820B (en) |
Families Citing this family (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH10204500A (en) * | 1997-01-20 | 1998-08-04 | Soft Kyukyu Corp:Kk | Effervescent tablet detergent |
AR015977A1 (en) * | 1997-10-23 | 2001-05-30 | Genencor Int | PROTEASA VARIANTS MULTIPLY SUBSTITUTED WITH ALTERED NET LOAD FOR USE IN DETERGENTS |
ES2368718T3 (en) | 1997-10-23 | 2011-11-21 | Danisco Us Inc. | SUBTILISINE VARIATIONS WITH MULTIPLE SUBSTITUTIONS. |
EP1045892A1 (en) * | 1997-12-22 | 2000-10-25 | The Procter & Gamble Company | Cleaning compositions containing a neopullulanase |
AU2001254623A1 (en) * | 2000-04-28 | 2001-11-12 | Novozymes A/S | Production and use of protein variants having modified immunogenicity |
US6803222B2 (en) * | 2000-11-22 | 2004-10-12 | Kao Corporation | Alkaline proteases |
DE10105911A1 (en) | 2001-02-09 | 2002-08-14 | Roche Diagnostics Gmbh | Expression of the recombinant proteinase K from Tritirachium album in yeast |
DE10105912A1 (en) * | 2001-02-09 | 2002-08-14 | Roche Diagnostics Gmbh | Recombinant Proteinase K |
DK200101090A (en) | 2001-07-12 | 2001-08-16 | Novozymes As | Subtilase variants |
US7888093B2 (en) | 2002-11-06 | 2011-02-15 | Novozymes A/S | Subtilase variants |
DE10260903A1 (en) * | 2002-12-20 | 2004-07-08 | Henkel Kgaa | New perhydrolases |
US7294499B2 (en) * | 2003-01-30 | 2007-11-13 | Novozymes A/S | Subtilases |
US7153666B2 (en) | 2003-07-17 | 2006-12-26 | General Atomics | Methods and compositions for determination of glycated proteins |
WO2008013874A1 (en) | 2006-07-25 | 2008-01-31 | General Atomics | Methods for assaying percentage of glycated hemoglobin |
US7943385B2 (en) | 2006-07-25 | 2011-05-17 | General Atomics | Methods for assaying percentage of glycated hemoglobin |
CN102076867A (en) * | 2008-05-13 | 2011-05-25 | 通用原子公司 | Electrochemical biosensor for direct determination of percentage of glycated hemoglobin |
CN105193640B (en) * | 2014-06-24 | 2018-10-12 | 金普诺安蛋白质工程技术(北京)有限公司 | Application of the Proteinase K in skin care and cosmetic field |
EP3380609B1 (en) | 2015-11-24 | 2020-11-11 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
CN109280656B (en) * | 2018-10-23 | 2021-06-15 | 大连博格林生物科技有限公司 | Recombinant beauveria bassiana proteinase K mutant PK-M1 and preparation method thereof |
CN109207460B (en) * | 2018-10-23 | 2021-06-15 | 大连博格林生物科技有限公司 | Recombinant beauveria bassiana proteinase K mutant PK-M2 and preparation method thereof |
US10633644B1 (en) * | 2018-12-20 | 2020-04-28 | New England Biolabs, Inc. | Proteinases with improved properties |
CN112592931A (en) * | 2020-12-31 | 2021-04-02 | 安徽丰原发酵技术工程研究有限公司 | Method for producing recombinant proteinase K |
CN113913412B (en) * | 2021-10-13 | 2023-10-03 | 湖北大学 | Proteinase K mutant and its preparing process |
CN114196656B (en) * | 2021-12-29 | 2023-10-24 | 南京巨匠生物科技有限公司 | Proteinase K mutant and construction and application of its expression vector |
CN115775591B (en) * | 2023-01-10 | 2023-06-09 | 华测检测认证集团股份有限公司 | Primer design method, device, equipment and readable storage medium |
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IE81141B1 (en) * | 1983-06-24 | 2000-04-05 | Genencor Int | Procaryotic carbonyl hydrolases |
US5310675A (en) * | 1983-06-24 | 1994-05-10 | Genencor, Inc. | Procaryotic carbonyl hydrolases |
US4760025A (en) | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
US5700676A (en) * | 1984-05-29 | 1997-12-23 | Genencor International Inc. | Modified subtilisins having amino acid alterations |
US5244791A (en) * | 1984-05-29 | 1993-09-14 | Genecor International, Inc. | Methods of ester hydrolysis |
US5182204A (en) * | 1984-05-29 | 1993-01-26 | Genencor International, Inc. | Non-human carbonyl hydrolase mutants, vectors encoding same and hosts transformed with said vectors |
US5346823A (en) * | 1984-05-29 | 1994-09-13 | Genencor, Inc. | Subtilisin modifications to enhance oxidative stability |
US5371008A (en) * | 1984-05-29 | 1994-12-06 | Genencor International, Inc. | Substrate assisted catalysis |
US5155033A (en) * | 1989-01-06 | 1992-10-13 | Genencor, Inc. | Subtilisins modified at position 225 resulting in a shift in catalytic activity |
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US6436690B1 (en) * | 1993-09-15 | 2002-08-20 | The Procter & Gamble Company | BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted |
DE69434962T2 (en) * | 1993-10-14 | 2008-01-17 | The Procter & Gamble Company, Cincinnati | PROTEASE-CONTAINING DETERGENTS |
DE69432954T2 (en) * | 1993-10-14 | 2004-04-22 | The Procter & Gamble Company, Cincinnati | BLUE COMPOSITION CONTAINING PROTEASES |
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US6599730B1 (en) * | 1994-05-02 | 2003-07-29 | Procter & Gamble Company | Subtilisin 309 variants having decreased adsorption and increased hydrolysis |
US5780285A (en) * | 1995-03-03 | 1998-07-14 | Genentech, Inc. | Subtilisin variants capable of cleaving substrates containing dibasic residues |
-
1996
- 1996-03-04 IL IL11735096A patent/IL117350A0/en unknown
- 1996-03-06 MX MX9706841A patent/MX9706841A/en unknown
- 1996-03-06 HU HU9801638A patent/HUP9801638A3/en unknown
- 1996-03-06 ZA ZA961820A patent/ZA961820B/en unknown
- 1996-03-06 CN CN96193792A patent/CN1183809A/en active Pending
- 1996-03-06 CA CA002214395A patent/CA2214395C/en not_active Expired - Fee Related
- 1996-03-06 CZ CZ972799A patent/CZ279997A3/en unknown
- 1996-03-06 AU AU50927/96A patent/AU5092796A/en not_active Abandoned
- 1996-03-06 JP JP8527690A patent/JPH11501814A/en active Pending
- 1996-03-06 WO PCT/US1996/003005 patent/WO1996028556A2/en not_active Application Discontinuation
- 1996-03-06 EP EP96907182A patent/EP0813601A2/en not_active Withdrawn
- 1996-03-06 BR BR9607677A patent/BR9607677A/en not_active Application Discontinuation
- 1996-03-07 MA MA24177A patent/MA23820A1/en unknown
-
1997
- 1997-09-23 US US08/935,887 patent/US6451574B1/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
HUP9801638A3 (en) | 2001-01-29 |
HUP9801638A2 (en) | 1998-10-28 |
JPH11501814A (en) | 1999-02-16 |
CA2214395C (en) | 2001-10-30 |
EP0813601A2 (en) | 1997-12-29 |
MA23820A1 (en) | 1996-10-01 |
WO1996028556A2 (en) | 1996-09-19 |
AU5092796A (en) | 1996-10-02 |
MX9706841A (en) | 1997-11-29 |
CN1183809A (en) | 1998-06-03 |
BR9607677A (en) | 1998-07-07 |
WO1996028556A3 (en) | 1997-07-17 |
CZ279997A3 (en) | 1998-01-14 |
ZA961820B (en) | 1996-09-10 |
US6451574B1 (en) | 2002-09-17 |
IL117350A0 (en) | 1996-07-23 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |