CA2217526A1 - Tunable excitation and/or tunable detection microplate reader - Google Patents

Tunable excitation and/or tunable detection microplate reader

Info

Publication number
CA2217526A1
CA2217526A1 CA002217526A CA2217526A CA2217526A1 CA 2217526 A1 CA2217526 A1 CA 2217526A1 CA 002217526 A CA002217526 A CA 002217526A CA 2217526 A CA2217526 A CA 2217526A CA 2217526 A1 CA2217526 A1 CA 2217526A1
Authority
CA
Canada
Prior art keywords
instrument
tunable
absorption
excitation
luminescence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002217526A
Other languages
French (fr)
Other versions
CA2217526C (en
Inventor
David M. Heffelfinger
Franklin R. Witney
Chris Cunanan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Bio-Rad Laboratories, Inc.
David M. Heffelfinger
Franklin R. Witney
Chris Cunanan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio-Rad Laboratories, Inc., David M. Heffelfinger, Franklin R. Witney, Chris Cunanan filed Critical Bio-Rad Laboratories, Inc.
Publication of CA2217526A1 publication Critical patent/CA2217526A1/en
Application granted granted Critical
Publication of CA2217526C publication Critical patent/CA2217526C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/251—Colorimeters; Construction thereof
    • G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths

Abstract

A method and apparatus of analyzing samples contained in a microplate is provided. The instrument is capable of measuring fluorescence, luminescence, and/or absorption within multiple locations within a sample well.
The instrument is tunable over the excitation and/or detection wavelengths. Neutral density filters are used to extend the sensitivity range of the absorption measuring aspect of the instrument. Due to the wavelength tuning capabilities of the instrument, the spectral dependence of the measured fluorescence, luminescence, and absorption of the materials in question can be analyzed. The combination of a data processor and a look-up table improve the ease of operation of the instrument. Several different formats are available for the output data including creation of a bit map of the sample.
CA002217526A 1996-10-11 1997-10-06 Tunable excitation and/or tunable detection microplate reader Expired - Fee Related CA2217526C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/729,111 US5784152A (en) 1995-03-16 1996-10-11 Tunable excitation and/or tunable detection microplate reader
US08/729,111 1996-10-11

Publications (2)

Publication Number Publication Date
CA2217526A1 true CA2217526A1 (en) 1998-04-11
CA2217526C CA2217526C (en) 2002-06-18

Family

ID=24929636

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002217526A Expired - Fee Related CA2217526C (en) 1996-10-11 1997-10-06 Tunable excitation and/or tunable detection microplate reader

Country Status (4)

Country Link
US (1) US5784152A (en)
EP (1) EP0841557A3 (en)
JP (1) JPH10170429A (en)
CA (1) CA2217526C (en)

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Also Published As

Publication number Publication date
EP0841557A2 (en) 1998-05-13
EP0841557A3 (en) 1999-04-07
CA2217526C (en) 2002-06-18
US5784152A (en) 1998-07-21
JPH10170429A (en) 1998-06-26

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