CA2220418A1 - Oligonucleotide sizing using cleavable primers - Google Patents

Oligonucleotide sizing using cleavable primers

Info

Publication number
CA2220418A1
CA2220418A1 CA002220418A CA2220418A CA2220418A1 CA 2220418 A1 CA2220418 A1 CA 2220418A1 CA 002220418 A CA002220418 A CA 002220418A CA 2220418 A CA2220418 A CA 2220418A CA 2220418 A1 CA2220418 A1 CA 2220418A1
Authority
CA
Canada
Prior art keywords
primer
released
sizing
cleavable
cleavable primers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002220418A
Other languages
French (fr)
Other versions
CA2220418C (en
Inventor
Joseph Albert Monforte
Christopher Hank Becker
Thomas Andrew Shaler
Daniel Joseph Pollart
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SRI International Inc
Original Assignee
Sri International
Joseph Albert Monforte
Christopher Hank Becker
Thomas Andrew Shaler
Daniel Joseph Pollart
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/445,751 external-priority patent/US5700642A/en
Application filed by Sri International, Joseph Albert Monforte, Christopher Hank Becker, Thomas Andrew Shaler, Daniel Joseph Pollart filed Critical Sri International
Publication of CA2220418A1 publication Critical patent/CA2220418A1/en
Application granted granted Critical
Publication of CA2220418C publication Critical patent/CA2220418C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6872—Methods for sequencing involving mass spectrometry
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/26—Mass spectrometers or separator tubes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

The present invention provides modified oligonucleotide primers designed to incorporate a cleavable moiety so that a (3') portion of the primer (linked to an extension product) can be released from an upstream (5') portion of the primer. Upon selective cleavage of the cleavable site, primer extension products that contain about five or fewer base pairs of the primer sequence are released, to provide more useful sizing and sequence information per fragment than extension products containing the entire primer.
CA002220418A 1995-05-22 1996-04-30 Oligonucleotide sizing using cleavable primers Expired - Fee Related CA2220418C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US08/445,751 1995-05-22
US08/445,751 US5700642A (en) 1995-05-22 1995-05-22 Oligonucleotide sizing using immobilized cleavable primers
US08/639,363 US5830655A (en) 1995-05-22 1996-04-26 Oligonucleotide sizing using cleavable primers
US08/639,363 1996-04-26
PCT/US1996/006116 WO1996037630A1 (en) 1995-05-22 1996-04-30 Oligonucleotide sizing using cleavable primers

Publications (2)

Publication Number Publication Date
CA2220418A1 true CA2220418A1 (en) 1996-11-28
CA2220418C CA2220418C (en) 2003-06-03

Family

ID=27034396

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002220418A Expired - Fee Related CA2220418C (en) 1995-05-22 1996-04-30 Oligonucleotide sizing using cleavable primers

Country Status (9)

Country Link
US (3) US5830655A (en)
EP (1) EP0828855B1 (en)
JP (1) JP3437184B2 (en)
KR (1) KR19990021950A (en)
CN (1) CN1191575A (en)
AU (1) AU695705B2 (en)
CA (1) CA2220418C (en)
DE (1) DE69605669T2 (en)
WO (1) WO1996037630A1 (en)

Families Citing this family (232)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6589726B1 (en) 1991-09-04 2003-07-08 Metrigen, Inc. Method and apparatus for in situ synthesis on a solid support
US5795714A (en) 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
EP1262564A3 (en) 1993-01-07 2004-03-31 Sequenom, Inc. Dna sequencing by mass spectrometry
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
US6194144B1 (en) 1993-01-07 2001-02-27 Sequenom, Inc. DNA sequencing by mass spectrometry
US6428955B1 (en) 1995-03-17 2002-08-06 Sequenom, Inc. DNA diagnostics based on mass spectrometry
US7803529B1 (en) 1995-04-11 2010-09-28 Sequenom, Inc. Solid phase sequencing of biopolymers
US5830655A (en) 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US6146854A (en) * 1995-08-31 2000-11-14 Sequenom, Inc. Filtration processes, kits and devices for isolating plasmids
US6051378A (en) * 1996-03-04 2000-04-18 Genetrace Systems Inc. Methods of screening nucleic acids using mass spectrometry
US6022688A (en) 1996-05-13 2000-02-08 Sequenom, Inc. Method for dissociating biotin complexes
US5853990A (en) 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
US5777324A (en) 1996-09-19 1998-07-07 Sequenom, Inc. Method and apparatus for maldi analysis
US5965363A (en) * 1996-09-19 1999-10-12 Genetrace Systems Inc. Methods of preparing nucleic acids for mass spectrometric analysis
US5885775A (en) * 1996-10-04 1999-03-23 Perseptive Biosystems, Inc. Methods for determining sequences information in polynucleotides using mass spectrometry
US6133436A (en) * 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
US6140053A (en) * 1996-11-06 2000-10-31 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
US7285422B1 (en) * 1997-01-23 2007-10-23 Sequenom, Inc. Systems and methods for preparing and analyzing low volume analyte array elements
US6024925A (en) 1997-01-23 2000-02-15 Sequenom, Inc. Systems and methods for preparing low volume analyte array elements
ATE259056T1 (en) 1996-11-06 2004-02-15 Sequenom Inc METHOD FOR MASS SPECTROMETRY
JP2001524808A (en) 1996-12-10 2001-12-04 ジーントレイス・システムズ・インコーポレイテッド Releasable non-volatile mass labeling molecules
DE19710166C1 (en) * 1997-03-12 1998-12-10 Bruker Franzen Analytik Gmbh Two-step method of DNA amplification for MALDI-TOF measurements
US6043070A (en) * 1997-08-29 2000-03-28 The Perkin-Elmer Corporation Phosphoramidate-phosphodiester oligonucleotide chimera as primers
US6207370B1 (en) 1997-09-02 2001-03-27 Sequenom, Inc. Diagnostics based on mass spectrometric detection of translated target polypeptides
US6268131B1 (en) 1997-12-15 2001-07-31 Sequenom, Inc. Mass spectrometric methods for sequencing nucleic acids
US6723564B2 (en) 1998-05-07 2004-04-20 Sequenom, Inc. IR MALDI mass spectrometry of nucleic acids using liquid matrices
DE19824280B4 (en) * 1998-05-29 2004-08-19 Bruker Daltonik Gmbh Mutation analysis using mass spectrometry
US6104028A (en) * 1998-05-29 2000-08-15 Genetrace Systems Inc. Volatile matrices for matrix-assisted laser desorption/ionization mass spectrometry
US6777188B2 (en) 1998-10-01 2004-08-17 Variagenics, Inc. Genotyping by mass spectrometric analysis of allelic fragments
US6566059B1 (en) * 1998-10-01 2003-05-20 Variagenics, Inc. Method for analyzing polynucleotides
US6610492B1 (en) 1998-10-01 2003-08-26 Variagenics, Inc. Base-modified nucleotides and cleavage of polynucleotides incorporating them
US6855500B2 (en) 1998-10-01 2005-02-15 Sequenom, Inc. Fluorescence-based genotyping
US6440705B1 (en) 1998-10-01 2002-08-27 Vincent P. Stanton, Jr. Method for analyzing polynucleotides
US6500650B1 (en) 1998-10-01 2002-12-31 Variagenics, Inc. Method for identifying polymorphisms
US6458945B1 (en) 1998-10-01 2002-10-01 Variagenics, Inc. Method for analyzing polynucleotides
US6994998B1 (en) 1998-10-01 2006-02-07 Sequenom, Inc. Base-modified nucleotides and their use for polymorphism detection
AU769759B2 (en) * 1998-10-26 2004-02-05 Yale University Allele frequency differences method for phenotype cloning
EP1133573A2 (en) * 1998-11-24 2001-09-19 The Johns Hopkins University Genotyping by mass spectrometric analysis of short dna fragments
WO2000053617A1 (en) * 1999-03-08 2000-09-14 Protogene Laboratories, Inc. Methods and compositions for economically synthesizing and assembling long dna sequences
US6844151B1 (en) * 1999-09-29 2005-01-18 Oligos Etc. Inc. Methods for production of arrays with modified oligonucleotide and polynucleotide compositions
EP1235932A2 (en) 1999-10-08 2002-09-04 Protogene Laboratories, Inc. Method and apparatus for performing large numbers of reactions using array assembly
JP2001245698A (en) * 1999-11-22 2001-09-11 Xiao Bing Wang Method for detecting nucleic acid
WO2001051689A1 (en) 2000-01-11 2001-07-19 Nanogen Recognomics Gmbh Biomolecules having multiple attachment moieties for binding to a substrate surface
EP1257664A4 (en) 2000-01-28 2006-04-05 Althea Technologies Inc Methods for analysis of gene expression
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
JP2003521252A (en) * 2000-02-07 2003-07-15 イルミナ インコーポレイテッド Nucleic acid detection method using universal priming
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US6911535B2 (en) * 2000-03-22 2005-06-28 Solvlink Biosciences Biomolecule/polymer conjugates
DE10015797B4 (en) * 2000-03-26 2006-02-02 Bruker Daltonik Gmbh Multiplex analysis of DNA mixtures using photolytically readable DNA chips
DE10021204A1 (en) * 2000-04-25 2001-11-08 Epigenomics Ag Method for the highly parallel analysis of polymorphisms
DE10123183A1 (en) * 2000-05-18 2001-11-22 Becton Dickinson Co Primer for strand-displacement primer extension, useful e.g. for detecting pathogenic bacteria, comprises two target-specific regions, flanking a non-binding region
EP1679381A1 (en) * 2000-06-08 2006-07-12 Xiao Bing Wang Isometric primers extension method and kit for detection and quantification of specific nucleic acid
US6824980B2 (en) * 2000-06-08 2004-11-30 Xiao Bing Wang Isometric primer extension method and kit for detection and quantification of specific nucleic acid
ATE423221T1 (en) 2000-06-13 2009-03-15 Univ Boston USE OF MASS-MATCHED NUCLEOTIDES IN THE ANALYSIS OF OLIGONUCLEOTIDE MIXTURES AND IN HIGH-MULTIPLEX NUCLEIC ACID SEQUENCING
DE60141087D1 (en) * 2000-06-26 2010-03-04 Nugen Technologies Inc METHODS AND COMPOSITIONS FOR TRANSCRIPTION-REPRODUCTIVE MACHINING OF NUCLEIC ACIDS
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US6994963B1 (en) * 2000-07-10 2006-02-07 Ambion, Inc. Methods for recombinatorial nucleic acid synthesis
US7129229B2 (en) 2000-08-11 2006-10-31 Nanogen Recognomics Gmbh Hydrazide building blocks and hydrazide modified biomolecules
AU2001280153A1 (en) * 2000-08-25 2002-03-04 Fujirebio Inc. Method of protecting personal information
DE10046184A1 (en) * 2000-09-18 2002-04-04 November Ag Molekulare Medizin Universally applicable method for parallel detection of nucleic acids, by forming a primer extension product that includes single-stranded segment for specific hybridization
US20020142483A1 (en) 2000-10-30 2002-10-03 Sequenom, Inc. Method and apparatus for delivery of submicroliter volumes onto a substrate
US6858413B2 (en) * 2000-12-13 2005-02-22 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
WO2002051537A2 (en) * 2000-12-22 2002-07-04 Burstein Technologies, Inc. Optical bio-discs and methods relating thereto
EP1359219A4 (en) * 2001-02-06 2004-09-22 Takara Bio Inc Amplified nucleic acids and immobilized products thereof
DE10108453B4 (en) * 2001-02-22 2005-10-20 Bruker Daltonik Gmbh Mass spectrometric mutation analysis with photolytically cleavable primers
US20040121314A1 (en) * 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents in containers
US20040121310A1 (en) * 2002-12-18 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents in forensic studies
US7666588B2 (en) 2001-03-02 2010-02-23 Ibis Biosciences, Inc. Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy
US20040121309A1 (en) 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents in blood, bodily fluids, and bodily tissues
US20030027135A1 (en) 2001-03-02 2003-02-06 Ecker David J. Method for rapid detection and identification of bioagents
US7226739B2 (en) 2001-03-02 2007-06-05 Isis Pharmaceuticals, Inc Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations
US7718354B2 (en) * 2001-03-02 2010-05-18 Ibis Biosciences, Inc. Methods for rapid identification of pathogens in humans and animals
BR0205268A (en) 2001-03-09 2004-11-30 Nugen Technologies Inc Processes and compositions for mRNA sequence mplification
EP1390537B1 (en) * 2001-03-09 2013-11-13 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
US8073627B2 (en) 2001-06-26 2011-12-06 Ibis Biosciences, Inc. System for indentification of pathogens
US7217510B2 (en) 2001-06-26 2007-05-15 Isis Pharmaceuticals, Inc. Methods for providing bacterial bioagent characterizing information
JP2003043037A (en) * 2001-07-27 2003-02-13 Inst Of Physical & Chemical Res Substrate for hybridization, manufacturing method and usage method of the substrate
US20050147984A1 (en) * 2001-08-31 2005-07-07 Clondiag Chip Technologies Gmbh Interaction detection on several probe arrays
DE10142643A1 (en) * 2001-08-31 2003-04-24 Clondiag Chip Tech Gmbh Detection of interactions on probe arrays
IL146270A0 (en) * 2001-11-01 2002-07-25 Yeda Res & Dev Pre-existing nucleic acids covalently attached to a metal surface or a metal cluster, intermediates thereof and methods of using same
US7510829B2 (en) * 2001-11-19 2009-03-31 Affymetrix, Inc. Multiplex PCR
US7223538B2 (en) * 2001-12-14 2007-05-29 Ge Healthcare Bio-Sciences Ab Post-synthesis labeling of nucleic acids, assays using nucleic acids that are labeled post-synthetically, single nucleotide polymorphism detection, and associated compounds and microarrays
DE10164219A1 (en) * 2001-12-31 2003-07-17 Reinald Repp Method for the detection of nucleic acids using solid phase-bound primers and cleavage of the products of a cyclic amplification reaction (Trap Release Primer Amplification (TRAmp)
EP1333101B1 (en) * 2002-02-01 2007-03-28 Bruker Daltonik GmbH Mutation analysis by PCR and Mass spectrometry
JP2003299489A (en) * 2002-02-08 2003-10-21 Mitsubishi Chemicals Corp Nucleic acid construct
EP1488001B1 (en) * 2002-03-11 2008-08-20 Nugen Technologies, Inc. Methods for generating double stranded dna comprising a 3' single stranded portion and uses of these complexes for recombination
US6723546B2 (en) * 2002-03-26 2004-04-20 New England Biolabs, Inc. Method for cloning and expression of BsaI restriction endonuclease and BsaI methylase in E. coli
ATE378424T1 (en) * 2002-08-12 2007-11-15 Gnothis Holding Sa DETECTION OF SINGLE NUCLEOTIDE POLYMORPHISMS BY SINGLE MOLECULE ANALYSIS
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
CA2508726A1 (en) 2002-12-06 2004-07-22 Isis Pharmaceuticals, Inc. Methods for rapid identification of pathogens in humans and animals
US20040185444A1 (en) * 2003-03-18 2004-09-23 Nicole Boehringer-Wyss System for predicting susceptibility of human oropharyngeal squamous cell carcinoma to radiotherapy
CA2521084A1 (en) 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
US8046171B2 (en) 2003-04-18 2011-10-25 Ibis Biosciences, Inc. Methods and apparatus for genetic evaluation
US8057993B2 (en) 2003-04-26 2011-11-15 Ibis Biosciences, Inc. Methods for identification of coronaviruses
US7964343B2 (en) 2003-05-13 2011-06-21 Ibis Biosciences, Inc. Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US8158354B2 (en) 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
EP1493824A1 (en) * 2003-07-02 2005-01-05 Consortium National de Recherche en Genomique (CNRG) Method for detection of mutations in DNA
US8546082B2 (en) 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US7956175B2 (en) 2003-09-11 2011-06-07 Ibis Biosciences, Inc. Compositions for use in identification of bacteria
WO2005029041A2 (en) * 2003-09-19 2005-03-31 Applera Corporation High density sequence detection methods and apparatus
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
ATE440948T1 (en) * 2004-02-04 2009-09-15 Qiagen North American Holdings IMPROVED NUCLEIC ACID AMPLIFICATION THROUGH ANTI-FROZEN PROTEIN
US7666592B2 (en) 2004-02-18 2010-02-23 Ibis Biosciences, Inc. Methods for concurrent identification and quantification of an unknown bioagent
US7981604B2 (en) * 2004-02-19 2011-07-19 California Institute Of Technology Methods and kits for analyzing polynucleotide sequences
US8119336B2 (en) 2004-03-03 2012-02-21 Ibis Biosciences, Inc. Compositions for use in identification of alphaviruses
WO2005117270A2 (en) * 2004-05-24 2005-12-08 Isis Pharmaceuticals, Inc. Mass spectrometry with selective ion filtration by digital thresholding
US20050266411A1 (en) 2004-05-25 2005-12-01 Hofstadler Steven A Methods for rapid forensic analysis of mitochondrial DNA
US7811753B2 (en) 2004-07-14 2010-10-12 Ibis Biosciences, Inc. Methods for repairing degraded DNA
WO2006135400A2 (en) 2004-08-24 2006-12-21 Isis Pharmaceuticals, Inc. Methods for rapid identification of recombinant organisms
US8084207B2 (en) 2005-03-03 2011-12-27 Ibis Bioscience, Inc. Compositions for use in identification of papillomavirus
WO2006094238A2 (en) 2005-03-03 2006-09-08 Isis Pharmaceuticals, Inc. Compositions for use in identification of adventitious viruses
US20060286572A1 (en) * 2005-05-16 2006-12-21 Kohne David E Method for producing chemically synthesized and in vitro enzymatically synthesized nucleic acid oligomers
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
CA2616281C (en) 2005-07-21 2014-04-22 Isis Pharmaceuticals, Inc. Methods for rapid identification and quantitation of mitochondrial dna variants
EP2385140B1 (en) * 2005-07-25 2014-07-16 Alere San Diego, Inc. Methods for multiplexing recombinase polymerase amplification
US7749701B2 (en) * 2005-08-11 2010-07-06 Agilent Technologies, Inc. Controlling use of oligonucleotide sequences released from arrays
WO2007030759A2 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
DE102005060925B4 (en) * 2005-12-14 2010-04-01 Universität Leipzig Method for the detection of DNA base sequence changes
US7625702B2 (en) * 2005-12-20 2009-12-01 International Business Machines Corporation Helical wrapping of single-walled carbon nanotubes by genomic DNA
US20070172839A1 (en) * 2006-01-24 2007-07-26 Smith Douglas R Asymmetrical adapters and methods of use thereof
DE102006007778B3 (en) * 2006-02-20 2007-11-08 Biotype Ag Method for analyzing multiplex mixtures consisting of nucleic acid amplification products with overlapping detection regions
EP2010679A2 (en) 2006-04-06 2009-01-07 Ibis Biosciences, Inc. Compositions for the use in identification of fungi
EP2029776A4 (en) * 2006-05-30 2010-01-20 Synergenz Bioscience Ltd Methods and compositions for assessment of pulmonary function and disorders
ATE502122T1 (en) * 2006-06-01 2011-04-15 Trilink Biotechnologies CHEMICALLY MODIFIED OLIGONUCLEOTIDE PRIMERS FOR NUCLEIC ACID AMPLIFICATION
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
AU2007353877B2 (en) 2006-09-14 2012-07-19 Ibis Biosciences, Inc. Targeted whole genome amplification method for identification of pathogens
WO2008045380A2 (en) * 2006-10-04 2008-04-17 Codon Devices, Inc. Nucleic acid libraries and their design and assembly
WO2008104002A2 (en) 2007-02-23 2008-08-28 Ibis Biosciences, Inc. Methods for rapid forensic dna analysis
US9598724B2 (en) 2007-06-01 2017-03-21 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
US20090036660A1 (en) * 2007-07-31 2009-02-05 Joel Myerson Methods and compositions for generating mixtures of nucleic acid molecules
WO2009039122A2 (en) 2007-09-17 2009-03-26 Sequenom, Inc. Integrated robotic sample transfer device
US20090075840A1 (en) * 2007-09-18 2009-03-19 Joel Myerson Methods And Compositions For Generating Mixtures Of Nucleic Acid Molecules
US20090203531A1 (en) 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
WO2009117698A2 (en) 2008-03-21 2009-09-24 Nugen Technologies, Inc. Methods of rna amplification in the presence of dna
WO2010025310A2 (en) 2008-08-27 2010-03-04 Westend Asset Clearinghouse Company, Llc Methods and devices for high fidelity polynucleotide synthesis
US8534447B2 (en) 2008-09-16 2013-09-17 Ibis Biosciences, Inc. Microplate handling systems and related computer program products and methods
US8148163B2 (en) 2008-09-16 2012-04-03 Ibis Biosciences, Inc. Sample processing units, systems, and related methods
WO2010033599A2 (en) 2008-09-16 2010-03-25 Ibis Biosciences, Inc. Mixing cartridges, mixing stations, and related kits, systems, and methods
EP2379748A4 (en) 2008-12-23 2012-08-29 Illumina Inc Multibase delivery for long reads in sequencing by synthesis protocols
EP2396803A4 (en) 2009-02-12 2016-10-26 Ibis Biosciences Inc Ionization probe assemblies
US9347092B2 (en) * 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
WO2010104798A1 (en) 2009-03-08 2010-09-16 Ibis Biosciences, Inc. Bioagent detection methods
CA2755615A1 (en) * 2009-03-18 2010-09-23 Sequenom, Inc. Use of thermostable endonucleases for generating reporter molecules
US9393564B2 (en) 2009-03-30 2016-07-19 Ibis Biosciences, Inc. Bioagent detection systems, devices, and methods
EP2454000A4 (en) 2009-07-17 2016-08-10 Ibis Biosciences Inc Systems for bioagent identification
US8950604B2 (en) 2009-07-17 2015-02-10 Ibis Biosciences, Inc. Lift and mount apparatus
WO2011014811A1 (en) 2009-07-31 2011-02-03 Ibis Biosciences, Inc. Capture primers and capture sequence linked solid supports for molecular diagnostic tests
US9080209B2 (en) 2009-08-06 2015-07-14 Ibis Biosciences, Inc. Non-mass determined base compositions for nucleic acid detection
EP2957641B1 (en) 2009-10-15 2017-05-17 Ibis Biosciences, Inc. Multiple displacement amplification
WO2011056872A2 (en) 2009-11-03 2011-05-12 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US20130203061A1 (en) 2009-11-30 2013-08-08 Michael KLASS Methods and systems for isolating, storing, and analyzing vesicles
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
JP5808349B2 (en) 2010-03-01 2015-11-10 カリス ライフ サイエンシズ スウィッツァーランド ホールディングスゲーエムベーハー Biomarkers for theranosis
US9758840B2 (en) 2010-03-14 2017-09-12 Ibis Biosciences, Inc. Parasite detection via endosymbiont detection
BR112012025593A2 (en) 2010-04-06 2019-06-25 Caris Life Sciences Luxembourg Holdings circulating biomarkers for disease
WO2011143556A1 (en) 2010-05-13 2011-11-17 Gen9, Inc. Methods for nucleotide sequencing and high fidelity polynucleotide synthesis
WO2011150168A1 (en) 2010-05-28 2011-12-01 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis
US8865404B2 (en) 2010-11-05 2014-10-21 President And Fellows Of Harvard College Methods for sequencing nucleic acid molecules
IL280133B2 (en) 2010-11-12 2023-04-01 Gen9 Inc Methods and devices for nucleic acids synthesis
US10457935B2 (en) 2010-11-12 2019-10-29 Gen9, Inc. Protein arrays and methods of using and making the same
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
SG188068A1 (en) * 2011-08-24 2013-03-28 Agency Science Tech & Res Method for detecting dna-binding proteins
ES2737957T3 (en) 2011-08-26 2020-01-17 Gen9 Inc Compositions and methods for high fidelity nucleic acid assembly
EP2766498B1 (en) 2011-10-14 2019-06-19 President and Fellows of Harvard College Sequencing by structure assembly
WO2013123125A1 (en) 2012-02-17 2013-08-22 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
WO2013130684A1 (en) 2012-02-27 2013-09-06 Amunix Operating Inc. Xten-folate conjugate compositions and methods of making same
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
EP2839031B1 (en) * 2012-04-19 2017-03-29 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent signaling oligonucleotide cleavage
CN104603286B (en) 2012-04-24 2020-07-31 Gen9股份有限公司 Method for sorting nucleic acids and multiplex preparations in vitro cloning
US8809435B2 (en) 2012-05-16 2014-08-19 The United States Of America As Represented By The Secretary Of The Army Process enhancement via stimuli responsive particle surfaces
EP2856177B1 (en) 2012-05-25 2020-11-18 The University of North Carolina At Chapel Hill Microfluidic devices, solid supports for reagents and related methods
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
LT2864531T (en) 2012-06-25 2019-03-12 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing
GB201215435D0 (en) * 2012-08-30 2012-10-17 Geneseque As Sequencing method
US9476089B2 (en) 2012-10-18 2016-10-25 President And Fellows Of Harvard College Methods of making oligonucleotide probes
US20160244816A1 (en) * 2013-07-15 2016-08-25 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent immobilized oligonucleotide hybridization
ES2943498T3 (en) 2013-08-05 2023-06-13 Twist Bioscience Corp de novo synthesized libraries
US11447814B2 (en) 2013-10-18 2022-09-20 Seegene, Inc. Detection of target nucleic acid sequence on solid phase by PTO cleavage and extension using HCTO assay
ES2925014T3 (en) 2014-09-12 2022-10-13 Univ Leland Stanford Junior Identification and use of circulating nucleic acids
CN105420225A (en) * 2014-09-17 2016-03-23 苏州旷世骏弛生物科技有限公司 Iodine dissociation type gene cloning method based on sulfo-modifiers
US20160083724A1 (en) * 2014-09-24 2016-03-24 University Of Southern California Methods for sample preparation
CN107208146B (en) * 2014-11-20 2022-01-18 安普里怀斯公司 Compositions and methods for nucleic acid amplification
EP3034619B1 (en) * 2014-12-16 2018-10-17 microBIOMix GmbH Modified oligonucleotides and method for feeding primers or control molecules or probes in closed PCR systems
CA2975855A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
US20170016894A1 (en) * 2015-07-15 2017-01-19 Orizhan Bioscience Limited Detection Comprising Signal Amplifier
EP3313573A2 (en) 2015-07-22 2018-05-02 The University of North Carolina at Chapel Hill Fluidic devices with bead well geometries with spatially separated bead retention and signal detection segments and related methods
GB2557529A (en) 2015-09-18 2018-06-20 Twist Bioscience Corp Oligonucleic acid variant libraries and synthesis thereof
WO2017053450A1 (en) 2015-09-22 2017-03-30 Twist Bioscience Corporation Flexible substrates for nucleic acid synthesis
WO2017095958A1 (en) 2015-12-01 2017-06-08 Twist Bioscience Corporation Functionalized surfaces and preparation thereof
KR101961642B1 (en) 2016-04-25 2019-03-25 (주)진매트릭스 A method for detecting target nucleic acid sequence using cleaved complementary tag fragment and a composition therefor
CN109996876A (en) 2016-08-22 2019-07-09 特韦斯特生物科学公司 The nucleic acid library of de novo formation
WO2018057526A2 (en) 2016-09-21 2018-03-29 Twist Bioscience Corporation Nucleic acid based data storage
SG10201709799SA (en) * 2016-11-29 2018-06-28 Helios Bioelectronics Inc Method for quantitatively profiling nucleic acids
AU2017378492B2 (en) 2016-12-16 2022-06-16 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018118971A1 (en) * 2016-12-19 2018-06-28 Bio-Rad Laboratories, Inc. Droplet tagging contiguity preserved tagmented dna
US11550939B2 (en) 2017-02-22 2023-01-10 Twist Bioscience Corporation Nucleic acid based data storage using enzymatic bioencryption
CA3056388A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018231872A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
EP3673064A4 (en) 2017-08-24 2021-05-26 Takara Bio USA, Inc. Methods of producing nucleic acids using oligonucleotides modified by a stimulus
SG11202002194UA (en) 2017-09-11 2020-04-29 Twist Bioscience Corp Gpcr binding proteins and synthesis thereof
KR20240024357A (en) 2017-10-20 2024-02-23 트위스트 바이오사이언스 코포레이션 Heated nanowells for polynucleotide synthesis
KR20200106067A (en) 2018-01-04 2020-09-10 트위스트 바이오사이언스 코포레이션 DNA-based digital information storage
EP3814497A4 (en) 2018-05-18 2022-03-02 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
JP7430701B2 (en) 2018-07-23 2024-02-13 ディーエヌエー スクリプト Large-scale parallel enzymatic synthesis of nucleic acid strands
JP2022515912A (en) 2019-01-03 2022-02-22 ディーエヌエー スクリプト One-pot synthesis of oligonucleotide sets
CN113785057A (en) 2019-02-26 2021-12-10 特韦斯特生物科学公司 Variant nucleic acid libraries for antibody optimization
CA3131689A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for glp1 receptor
AU2020298294A1 (en) 2019-06-21 2022-02-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
WO2021018919A1 (en) 2019-07-30 2021-02-04 Dna Script Template-free enzymatic synthesis of polynucleotides using poly(a) and poly(u) polymerases
KR20220052938A (en) 2019-08-01 2022-04-28 디엔에이 스크립트 Increased Long Sequence Yield in Template-Free Enzymatic Synthesis of Polynucleotides
CN114729389B (en) 2019-09-23 2024-03-08 Dna斯克瑞普特公司 Increasing long sequence yield in template-free enzymatic synthesis of polynucleotides
US11873484B2 (en) 2019-11-27 2024-01-16 Microsoft Technology Licensing, Llc Oligonucleotide assembly using electrically controlled hybridization
US20210205775A1 (en) * 2020-01-06 2021-07-08 Microsoft Technology Licensing, Llc Solid-phase polymer synthesis on reusable substrates
EP4110940B1 (en) 2020-02-25 2024-03-27 DNA Script Method and apparatus for enzymatic synthesis of polynucleotides
EP4139446A1 (en) 2020-04-20 2023-03-01 DNA Script Terminal deoxynucleotidyl transferase variants and uses thereof
EP4164784A1 (en) 2020-06-16 2023-04-19 DNA Script Systems, apparatus and kits for enzymatic polynucleotide synthesis
US20230313255A1 (en) 2020-07-15 2023-10-05 Dna Script Massively Parallel Enzymatic Synthesis of Polynucleotides
EP4217476A1 (en) 2020-09-22 2023-08-02 DNA Script Stabilized n-terminally truncated terminal deoxynucleotidyl transferase variants and uses thereof
CN112941147A (en) * 2021-03-02 2021-06-11 深圳市睿法生物科技有限公司 High-fidelity target gene library building method and kit thereof
JP2024511874A (en) 2021-04-02 2024-03-15 ディーエヌエー スクリプト Method and kit for enzymatic synthesis of polynucleotides susceptible to G4 formation
WO2023035003A1 (en) 2021-09-03 2023-03-09 Elegen Corp. Multi-way bead-sorting devices, systems, and methods of use thereof using pressure sources
WO2023170286A2 (en) 2022-03-11 2023-09-14 Dna Script Alignment post and secure mechanism for enzymatic polynucleotide synthesis
WO2023170266A1 (en) 2022-03-11 2023-09-14 Dna Script Automation station for enzymatic polynucleotide synthesis
WO2023170259A1 (en) 2022-03-11 2023-09-14 Dna Script Modular accessory rack
WO2023170258A1 (en) 2022-03-11 2023-09-14 Dna Script Apparatus for enzymatic synthesis of a plurality of polynucleotides comprising a condensation trap

Family Cites Families (102)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4139346A (en) 1977-11-28 1979-02-13 Enzo Bio Chem Incorporated Nucleic acid and protein binding paper
US4711955A (en) 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
US5198540A (en) 1982-10-28 1993-03-30 Hubert Koster Process for the preparation of oligonucleotides in solution
US5059654A (en) 1983-02-14 1991-10-22 Cuno Inc. Affinity matrices of modified polysaccharide supports
US4515781A (en) 1983-02-23 1985-05-07 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services 2',5'-Riboadenylate-morpholinoadenylate nucleotides
JPS59161398A (en) * 1983-03-02 1984-09-12 Mitsui Toatsu Chem Inc Method and apparatus for incising deoxyribonucleic acid
GB8311018D0 (en) 1983-04-22 1983-05-25 Amersham Int Plc Detecting mutations in dna
DE3329892A1 (en) 1983-08-18 1985-03-07 Köster, Hubert, Prof. Dr., 2000 Hamburg METHOD FOR PRODUCING OLIGONUCLEOTIDES
US4582789A (en) 1984-03-21 1986-04-15 Cetus Corporation Process for labeling nucleic acids using psoralen derivatives
US4729947A (en) 1984-03-29 1988-03-08 The Board Of Regents Of The University Of Nebraska DNA sequencing
US4957858A (en) 1986-04-16 1990-09-18 The Salk Instute For Biological Studies Replicative RNA reporter systems
US4683194A (en) 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4849077A (en) 1984-08-06 1989-07-18 Akademie Der Wissenschaften Der Ddr Process for solid phase-sequencing of nucleic acid fragments
US4775619A (en) * 1984-10-16 1988-10-04 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US5430136A (en) * 1984-10-16 1995-07-04 Chiron Corporation Oligonucleotides having selectably cleavable and/or abasic sites
US5118605A (en) * 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US4604363A (en) 1984-10-18 1986-08-05 Analytical Bio-Chemistry Laboratories Inc. Automatic evaporator system
US5064754A (en) 1984-12-14 1991-11-12 Mills Randell L Genomic sequencing method
US5221518A (en) 1984-12-14 1993-06-22 Mills Randell L DNA sequencing apparatus
US4818681A (en) * 1985-02-22 1989-04-04 Molecular Diagnostics, Inc. Fast and specific immobilization of nucleic acids to solid supports
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
GB8515276D0 (en) * 1985-06-17 1985-07-17 Amersham Int Plc Nucleic acid sequencing
US4806546A (en) 1985-09-30 1989-02-21 Miles Inc. Immobilization of nucleic acids on derivatized nylon supports
US4998617A (en) 1986-09-15 1991-03-12 Laura Lupton Inc Facial cosmetic liquid make up kit
US5175209A (en) 1987-01-06 1992-12-29 Baylor College Of Medicine Porous wafer for segmented synthesis of biopolymers
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US5149625A (en) 1987-08-11 1992-09-22 President And Fellows Of Harvard College Multiplex analysis of DNA
US5403711A (en) 1987-11-30 1995-04-04 University Of Iowa Research Foundation Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved
US5670381A (en) 1988-01-29 1997-09-23 Abbott Laboratories Devices for performing ion-capture binding assays
DE3809504C1 (en) 1988-03-22 1989-09-21 Bruker - Franzen Analytik Gmbh, 2800 Bremen, De
SE8801070D0 (en) * 1988-03-23 1988-03-23 Pharmacia Ab METHOD FOR IMMOBILIZING A DNA SEQUENCE ON A SOLID SUPPORT
US5266684A (en) 1988-05-02 1993-11-30 The Reagents Of The University Of California Peptide mixtures
US5003059A (en) * 1988-06-20 1991-03-26 Genomyx, Inc. Determining DNA sequences by mass spectrometry
FR2636738B1 (en) * 1988-09-20 1992-12-11 Commissariat Energie Atomique DNA SEQUENCING PROCESS AND INSTALLATION
DE3930312A1 (en) * 1988-10-24 1990-04-26 Max Planck Gesellschaft METHOD FOR DIRECTLY SEQUENCING NUCLEIC ACID CHAINS
US5512439A (en) 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
US5237016A (en) 1989-01-05 1993-08-17 Siska Diagnostics, Inc. End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids
US4920264A (en) 1989-01-17 1990-04-24 Sri International Method for preparing samples for mass analysis by desorption from a frozen solution
US5459039A (en) * 1989-05-12 1995-10-17 Duke University Methods for mapping genetic mutations
US5242974A (en) 1991-11-22 1993-09-07 Affymax Technologies N.V. Polymer reversal on solid surfaces
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
GB2236186B (en) 1989-08-22 1994-01-05 Finnigan Mat Gmbh Process and device for laser desorption of analyte molecular ions, especially of biomolecules
WO1991011533A1 (en) * 1990-01-26 1991-08-08 E.I. Du Pont De Nemours And Company Method for isolating primer extension products from template-directed dna polymerase reactions
US5288644A (en) * 1990-04-04 1994-02-22 The Rockefeller University Instrument and method for the sequencing of genome
CA2036946C (en) 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
HU218095B (en) * 1990-05-01 2000-05-28 Amgen Inc. Process for reducing transitional contaminations in amplification processes
US5135870A (en) 1990-06-01 1992-08-04 Arizona Board Of Regents Laser ablation/ionizaton and mass spectrometric analysis of massive polymers
US5650489A (en) 1990-07-02 1997-07-22 The Arizona Board Of Regents Random bio-oligomer library, a method of synthesis thereof, and a method of use thereof
TW221493B (en) 1990-07-10 1994-03-01 Cardiovascular Diagnostics Inc
DE4024545A1 (en) 1990-08-02 1992-02-06 Boehringer Mannheim Gmbh Metered delivery of biochemical analytical soln., esp. reagent
ATE151815T1 (en) * 1990-09-21 1997-05-15 Amgen Inc ENZYMATIC SYNTHESIS OF OLIGONUCLEOTIDES
US5846710A (en) 1990-11-02 1998-12-08 St. Louis University Method for the detection of genetic diseases and gene sequence variations by single nucleotide primer extension
US5210412A (en) 1991-01-31 1993-05-11 Wayne State University Method for analyzing an organic sample
WO1992013629A1 (en) 1991-01-31 1992-08-20 Wayne State University A method for analyzing an organic sample
US5762876A (en) 1991-03-05 1998-06-09 Molecular Tool, Inc. Automatic genotype determination
US5300774A (en) 1991-04-25 1994-04-05 Applied Biosystems, Inc. Time-of-flight mass spectrometer with an aperture enabling tradeoff of transmission efficiency and resolution
EP0823486A3 (en) * 1991-06-27 2004-02-11 Genelabs Technologies, Inc. Method for inhibiting the binding of a dna-binding protein to duplex dna
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US5605662A (en) 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
IL103674A0 (en) 1991-11-19 1993-04-04 Houston Advanced Res Center Method and apparatus for molecule detection
DE69233331T3 (en) 1991-11-22 2007-08-30 Affymetrix, Inc., Santa Clara Combinatorial Polymersynthesis Strategies
ATE192503T1 (en) * 1991-12-24 2000-05-15 Tepnel Medical Ltd MANIPULATION OF NUCLEIC ACID SEQUENCES
WO1993020236A1 (en) 1992-04-03 1993-10-14 Applied Biosystems, Inc. Probe composition and method
US5710028A (en) 1992-07-02 1998-01-20 Eyal; Nurit Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor
US5503980A (en) 1992-11-06 1996-04-02 Trustees Of Boston University Positional sequencing by hybridization
US5795714A (en) 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
US5436143A (en) 1992-12-23 1995-07-25 Hyman; Edward D. Method for enzymatic synthesis of oligonucleotides
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
EP1262564A3 (en) * 1993-01-07 2004-03-31 Sequenom, Inc. Dna sequencing by mass spectrometry
US5571669A (en) 1993-01-14 1996-11-05 The University Of Pennsylvania Transcription and nucleic acid sequence determination with short primer DNA/RNA molecules and RNA polymerase
DE614989T1 (en) 1993-02-17 1995-09-28 Morphosys Proteinoptimierung Method for in vivo selection of ligand binding proteins.
EP0612994A3 (en) 1993-02-26 1996-03-06 Ciba Geigy Ag Matrix for matrix-assisted laser desorption mass spectroscopy.
CA2158642A1 (en) 1993-03-19 1994-09-29 Hubert Koster Dna sequencing by mass spectrometry via exonuclease degradation
US5593826A (en) 1993-03-22 1997-01-14 Perkin-Elmer Corporation, Applied Biosystems, Inc. Enzymatic ligation of 3'amino-substituted oligonucleotides
US5376788A (en) 1993-05-26 1994-12-27 University Of Manitoba Apparatus and method for matrix-assisted laser desorption mass spectrometry
GB9315847D0 (en) 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
US5527675A (en) 1993-08-20 1996-06-18 Millipore Corporation Method for degradation and sequencing of polymers which sequentially eliminate terminal residues
WO1995009688A1 (en) 1993-10-04 1995-04-13 Hewlett-Packard Company Sample preparation system and method
US5622829A (en) 1993-12-08 1997-04-22 The Regents Of The University Of California Genetic markers for breast, ovarian, and prostatic cancer
US5538897A (en) 1994-03-14 1996-07-23 University Of Washington Use of mass spectrometry fragmentation patterns of peptides to identify amino acid sequences in databases
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5834189A (en) 1994-07-08 1998-11-10 Visible Genetics Inc. Method for evaluation of polymorphic genetic sequences, and the use thereof in identification of HLA types
US5498545A (en) 1994-07-21 1996-03-12 Vestal; Marvin L. Mass spectrometer system and method for matrix-assisted laser desorption measurements
US5763594A (en) * 1994-09-02 1998-06-09 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US5512295A (en) 1994-11-10 1996-04-30 The Board Of Trustees Of The Leland Stanford Junior University Synthetic liposomes for enhanced uptake and delivery
US5688642A (en) 1994-12-01 1997-11-18 The United States Of America As Represented By The Secretary Of The Navy Selective attachment of nucleic acid molecules to patterned self-assembled surfaces
US5601982A (en) 1995-02-07 1997-02-11 Sargent; Jeannine P. Method and apparatus for determining the sequence of polynucleotides
US5609907A (en) 1995-02-09 1997-03-11 The Penn State Research Foundation Self-assembled metal colloid monolayers
US5827659A (en) 1995-05-19 1998-10-27 Perseptive Biosystems, Inc. Methods and apparatus for sequencing polymers using mass spectrometry
US5625184A (en) 1995-05-19 1997-04-29 Perseptive Biosystems, Inc. Time-of-flight mass spectrometry analysis of biomolecules
US5830655A (en) 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US5700642A (en) 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
US5654150A (en) 1995-06-07 1997-08-05 President And Fellows Of Harvard College Method of expression cloning
US5571676A (en) 1995-06-07 1996-11-05 Ig Laboratories, Inc. Method for mismatch-directed in vitro DNA sequencing
US5589136A (en) 1995-06-20 1996-12-31 Regents Of The University Of California Silicon-based sleeve devices for chemical reactions
US5716825A (en) 1995-11-01 1998-02-10 Hewlett Packard Company Integrated nucleic acid analysis system for MALDI-TOF MS
US5580434A (en) 1996-02-29 1996-12-03 Hewlett-Packard Company Interface apparatus for capillary electrophoresis to a matrix-assisted-laser-desorption-ionization mass spectrometer
DE19617011C2 (en) 1996-04-27 2000-11-02 Bruker Daltonik Gmbh Matrix component mixture for matrix-assisted laser desorption and ionization and method for preparing a matrix component mixture
DE19628178C1 (en) 1996-07-12 1997-09-18 Bruker Franzen Analytik Gmbh Loading matrix-assisted laser desorption-ionisation sample plate for mass spectrometric analysis
US5777324A (en) 1996-09-19 1998-07-07 Sequenom, Inc. Method and apparatus for maldi analysis

Also Published As

Publication number Publication date
CA2220418C (en) 2003-06-03
JP3437184B2 (en) 2003-08-18
US5830655A (en) 1998-11-03
AU5635296A (en) 1996-12-11
US20060040282A1 (en) 2006-02-23
JPH11505127A (en) 1999-05-18
DE69605669D1 (en) 2000-01-20
DE69605669T2 (en) 2000-07-27
KR19990021950A (en) 1999-03-25
EP0828855A1 (en) 1998-03-18
US6949633B1 (en) 2005-09-27
WO1996037630A1 (en) 1996-11-28
AU695705B2 (en) 1998-08-20
EP0828855B1 (en) 1999-12-15
CN1191575A (en) 1998-08-26

Similar Documents

Publication Publication Date Title
CA2220418A1 (en) Oligonucleotide sizing using cleavable primers
AU6589094A (en) 7-deazapurine modified oligonucleotides
AU1925195A (en) Nucleic acid delivery system, method of synthesis and uses thereof
PL321380A1 (en) Novel far nesil transferase inhibitors, their production and pharmaceutic compositions containing them
CA2153898A1 (en) Method for generating single-stranded dna molecules
AU5121399A (en) Rna targeted 2'-modified oligonucleotides that are conformationally preorganized
PL324430A1 (en) Novel inhibitors of pharnesiltransferase, their production and pharmaceutic compositions containing them
PL321710A1 (en) Novel inhibitors of farnseferase, their production and pharmaceutic compositions containing them
CA2230682A1 (en) Sugar-free dragee-like products
HUP9802422A3 (en) Substituted isoquinoline-3-carboxamide derivatives, process for producing them and pharmaceutical compositions containing them
AU1180997A (en) Novel taxoids, their preparation and pharmaceutical compositions containing them
WO1999049082A3 (en) Modified nucleotides and methods useful for nucleic acid sequencing
ZA965294B (en) Substituted 4-phenylaminothiazoles, their process of preparation and the pharmaceutical compositions containing them.
AU6101196A (en) Seed coating containing mn(no3)2.6h2o
IL119488A0 (en) Substituted 2,4-imidazolidinediones their production and pharmaceutical compositions containing them
WO1996037605A3 (en) Anti sense oligonucleotides for blocking ige receptor synthesis
WO1996021468A3 (en) Chemically modified interferon
AU7653691A (en) Modified rna template-specific polymerase chain reaction
AU1062997A (en) Novel polymer supports for nucleic acid synthesis
WO1995006764A3 (en) Oligonucleotides with rna cleavage activity
WO2000029623A3 (en) Nucleic acids containing single nucleotide polymorphisms and methods of use thereof
HU9601321D0 (en) Substituted (benzyloxy)-carbonyl-guanidine derivatives, process for producing them, their use as pharmaceutical compositions or diagnostica, and pharmaceutical compositions containing them
AU8406298A (en) Production of galactomannan products by enzymatic reaction on guar splits
ZA9610460B (en) New phenylamidine derivatives, processes for preparing them and their use as pharmaceutical compositions.
AU6934398A (en) A process for the synthesis of modified p-chiral nucleotide analogues

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20160502