CA2223729A1 - Universal primer sequence for multiplex dna amplification - Google Patents

Universal primer sequence for multiplex dna amplification

Info

Publication number
CA2223729A1
CA2223729A1 CA002223729A CA2223729A CA2223729A1 CA 2223729 A1 CA2223729 A1 CA 2223729A1 CA 002223729 A CA002223729 A CA 002223729A CA 2223729 A CA2223729 A CA 2223729A CA 2223729 A1 CA2223729 A1 CA 2223729A1
Authority
CA
Canada
Prior art keywords
dna
primer sequence
dna amplification
present
universal primer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002223729A
Other languages
French (fr)
Other versions
CA2223729C (en
Inventor
Anthony P. Shuber
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Esoterix Genetic Laboratories LLC
Original Assignee
Genzyme Corporation
Anthony P. Shuber
Ig Laboratories, Inc.
Esoterix Genetic Laboratories, Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23883585&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2223729(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genzyme Corporation, Anthony P. Shuber, Ig Laboratories, Inc., Esoterix Genetic Laboratories, Llc filed Critical Genzyme Corporation
Publication of CA2223729A1 publication Critical patent/CA2223729A1/en
Application granted granted Critical
Publication of CA2223729C publication Critical patent/CA2223729C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Abstract

The present invention provides primers that allow simultaneous amplification of multiple DNA target sequences present in a DNA
sample. Further provided are methods for detecting multiple defined target DNA
sequences in a DNA sample. Methods for high-throughput genetic screening are also provided. In yet another aspect, the present invention provides single-stranded oligonucleotide DNA primers for amplification of a target DNA sequence in a multiplex polymerase chain reaction.
CA002223729A 1995-06-07 1996-06-06 Universal primer sequence for multiplex dna amplification Expired - Fee Related CA2223729C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/474,450 US5882856A (en) 1995-06-07 1995-06-07 Universal primer sequence for multiplex DNA amplification
US08/474,450 1995-06-07
PCT/US1996/009637 WO1996041012A1 (en) 1995-06-07 1996-06-06 Universal primer sequence for multiplex dna amplification

Publications (2)

Publication Number Publication Date
CA2223729A1 true CA2223729A1 (en) 1996-12-19
CA2223729C CA2223729C (en) 2004-04-20

Family

ID=23883585

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002223729A Expired - Fee Related CA2223729C (en) 1995-06-07 1996-06-06 Universal primer sequence for multiplex dna amplification

Country Status (7)

Country Link
US (2) US5882856A (en)
EP (1) EP0832290B1 (en)
JP (1) JPH11507226A (en)
AT (1) ATE232242T1 (en)
CA (1) CA2223729C (en)
DE (1) DE69626111T2 (en)
WO (1) WO1996041012A1 (en)

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Also Published As

Publication number Publication date
ATE232242T1 (en) 2003-02-15
JPH11507226A (en) 1999-06-29
AU6163696A (en) 1996-12-30
EP0832290B1 (en) 2003-02-05
DE69626111D1 (en) 2003-03-13
US6207372B1 (en) 2001-03-27
AU703417B2 (en) 1999-03-25
WO1996041012A1 (en) 1996-12-19
US5882856A (en) 1999-03-16
EP0832290A1 (en) 1998-04-01
DE69626111T2 (en) 2003-12-24
CA2223729C (en) 2004-04-20

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