CA2239418A1 - Screening natural samples for new therapeutic compounds using capillary electrophoresis - Google Patents

Screening natural samples for new therapeutic compounds using capillary electrophoresis

Info

Publication number
CA2239418A1
CA2239418A1 CA002239418A CA2239418A CA2239418A1 CA 2239418 A1 CA2239418 A1 CA 2239418A1 CA 002239418 A CA002239418 A CA 002239418A CA 2239418 A CA2239418 A CA 2239418A CA 2239418 A1 CA2239418 A1 CA 2239418A1
Authority
CA
Canada
Prior art keywords
ligands
target molecule
compounds
charged
bind
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002239418A
Other languages
French (fr)
Other versions
CA2239418C (en
Inventor
Dallas E. Hughes
Barry L. Karger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Northeastern University Boston
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2239418A1 publication Critical patent/CA2239418A1/en
Application granted granted Critical
Publication of CA2239418C publication Critical patent/CA2239418C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G01N33/561—Immunoelectrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Abstract

A method in which natural sample components are simultaneously fractionated and screened for compounds that bind tightly to specific molecules of interest is disclosed. Such newly isolated ligands are good candidates for potential therapeutic or diagnostic compounds. The natural sample is first combined with a potential target molecule and then subjected to capillary eiectrophoresis (CE). Charged (or even neutral) compounds present in the natural sample that bind to the added target molecule can alter its normal migration time upon CE, by changing its charge-to-mass ratio, or will cause a variation in peak shape or area. Complex formation can lie detected by simply monitoring the migration of the target molecule during electrophoresis. Any new ligands that bind to the target molecule wilt be good candidates for therapeutic or diagnostic compounds. Interfering, weak-binding ligands commonly present in crude extracts are not detected. Small, neutral ligands, as well as charged ligands, can be identified in competitive binding experiments with known, charged competitor molecules.
CA002239418A 1995-12-11 1996-12-10 Screening natural samples for new therapeutic compounds using capillary electrophoresis Expired - Fee Related CA2239418C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US850395P 1995-12-11 1995-12-11
US60/008,503 1995-12-11
US08/662,085 US5783397A (en) 1995-12-11 1996-06-12 Screening natural samples for new therapeutic compounds using capillary electrophoresis
US08/662,085 1996-06-12
PCT/US1996/019779 WO1997022000A1 (en) 1995-12-11 1996-12-10 Screening natural samples for new therapeutic compounds using capillary electrophoresis

Publications (2)

Publication Number Publication Date
CA2239418A1 true CA2239418A1 (en) 1997-06-19
CA2239418C CA2239418C (en) 2005-09-20

Family

ID=26678258

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002239418A Expired - Fee Related CA2239418C (en) 1995-12-11 1996-12-10 Screening natural samples for new therapeutic compounds using capillary electrophoresis

Country Status (8)

Country Link
US (1) US5783397A (en)
EP (1) EP0876609B1 (en)
JP (1) JP3603267B2 (en)
CA (1) CA2239418C (en)
DE (1) DE69624942T2 (en)
DK (1) DK0876609T3 (en)
ES (1) ES2187685T3 (en)
WO (1) WO1997022000A1 (en)

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US6528098B2 (en) * 1996-10-22 2003-03-04 Advanced Viral Research Corp. Preparation of a therapeutic composition
US6455323B1 (en) * 1997-07-03 2002-09-24 Pharmacia & Upjohn Company Anti-bacterial methods and materials
US7745142B2 (en) 1997-09-15 2010-06-29 Molecular Devices Corporation Molecular modification assays
US6299747B1 (en) * 1997-12-17 2001-10-09 Cetek Corporation Capillary electrophoretic methods to detect new biologically active compounds in complex biological material
WO1999034203A1 (en) * 1997-12-24 1999-07-08 Cetek Corporation Capillary electrophoretic method to detect target-binding ligands and to determine their relative affinities
WO2000003240A1 (en) * 1998-07-10 2000-01-20 Cetek Corporation Method to detect and analyze tight-binding ligands in complex biological samples
US6432651B1 (en) 1998-07-10 2002-08-13 Cetek Corporation Method to detect and analyze tight-binding ligands in complex biological samples using capillary electrophoresis and mass spectrometry
US6132685A (en) * 1998-08-10 2000-10-17 Caliper Technologies Corporation High throughput microfluidic systems and methods
US20020035459A1 (en) 1998-09-14 2002-03-21 George M. Grass Pharmacokinetic-based drug design tool and method
WO2000043787A1 (en) * 1999-01-26 2000-07-27 Du Pont Pharmaceuticals Company Electrophoretic assays to assess conformational changes of integrins induced by ligand binding
US6329145B1 (en) * 1999-02-09 2001-12-11 Gilead Science, Inc. Determining non-nucleic acid molecule binding to target by competition with nucleic acid ligand
CA2362052A1 (en) 1999-02-12 2000-08-17 Cetek Corporation High throughput size-exclusive method of screening complex biological materials for affinity ligands
US6306628B1 (en) * 1999-08-25 2001-10-23 Ambergen, Incorporated Methods for the detection, analysis and isolation of Nascent proteins
US7217573B1 (en) * 1999-10-05 2007-05-15 Hitachi, Ltd. Method of inspecting a DNA chip
EP1360315A2 (en) 2000-04-06 2003-11-12 PHARMACIA & UPJOHN COMPANY Antimicrobial methods and materials
US7252932B1 (en) 2000-08-23 2007-08-07 Ambergen, Inc. Methods for the detection, analysis and isolation of nascent proteins
EP1720011A1 (en) 2000-12-01 2006-11-08 Cetek Corporation High throughput capillary electrophoresis system
US20030132117A1 (en) * 2002-01-14 2003-07-17 Andras Guttman Thin film electrophoresis apparatus and method
US20040266021A9 (en) * 2001-01-26 2004-12-30 Andras Guttman Multicapillary fraction collection system and method
US20030087321A1 (en) * 2001-09-28 2003-05-08 Tomich Che-Shen C. Antimicrobial methods and materials
US20050272089A1 (en) * 2001-10-19 2005-12-08 Mott John E Critical genes and polypeptides of haemophilus influenzae and methods of use
US20030220249A1 (en) * 2002-02-07 2003-11-27 Hackett Perry B. Factors for angiogenesis, vasculogenesis, cartilage formation, bone formation, and methods of use thereof
GB2409038B (en) * 2002-10-09 2006-04-05 Waters Investments Ltd Methods and apparatus for identifying compounds in a sample
US7672786B2 (en) 2003-07-02 2010-03-02 Sergey Krylov Non-equilibrium capillary electrophoresis of equilibrium mixtures (NECEEM)—based methods for drug and diagnostic development
JP4512745B2 (en) * 2004-10-29 2010-07-28 独立行政法人産業技術総合研究所 Cell separation and identification apparatus and method
JP2008542369A (en) * 2005-06-03 2008-11-27 アドバンスト バイラル リサーチ コープ． Method for providing palliative care using AVR118
US7959861B2 (en) * 2007-09-19 2011-06-14 Stc.Unm Integrated affinity microcolumns and affinity capillary electrophoresis
JP5498399B2 (en) 2008-02-12 2014-05-21 ヒューレット−パッカード デベロップメント カンパニー エル．ピー． Color detector
JP2011511946A (en) 2008-02-13 2011-04-14 ヒューレット−パッカード デベロップメント カンパニー エル．ピー． COLOR DETECTOR HAVING OPTICAL DETECTOR WITH ENLARGED AREA
BRPI0901112A2 (en) * 2009-03-12 2010-11-16 Luiz Augusto Pinto high affinity and high specificity oligonucleotide production process for organic and inorganic molecules
WO2010141131A1 (en) 2009-06-04 2010-12-09 Lockheed Martin Corporation Multiple-sample microfluidic chip for dna analysis
AU2011315951B2 (en) 2010-10-15 2015-03-19 Lockheed Martin Corporation Micro fluidic optic design
US9322054B2 (en) 2012-02-22 2016-04-26 Lockheed Martin Corporation Microfluidic cartridge

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WO1991006850A1 (en) * 1989-11-06 1991-05-16 Massachusetts Institute Of Technology Affinity co-electrophoresis
WO1992013972A1 (en) * 1991-02-11 1992-08-20 Thomas Jefferson University Genetically engineered bacteria to identify and produce medically important agents
AU647929B2 (en) * 1991-03-28 1994-03-31 Perseptive Biosystems, Inc. On-line product identification in a chromatography effluent by subtraction
US5413686A (en) * 1992-07-17 1995-05-09 Beckman Instruments, Inc. Multi-channel automated capillary electrophoresis analyzer
US5348633A (en) * 1993-01-22 1994-09-20 Northeastern University Method for quantitating trace amounts of an analyte in a sample by affinity capillary electrophoresis
CA2154882A1 (en) * 1993-01-28 1994-08-04 Robert Tjian Tata-binding protein associated factors, nucleic acids encoding tafs, and methods of use
US5324401A (en) * 1993-02-05 1994-06-28 Iowa State University Research Foundation, Inc. Multiplexed fluorescence detector system for capillary electrophoresis
US5567282A (en) * 1994-01-25 1996-10-22 Wang; Hann-Ping On-capillary electrophoretic immunosubtraction for classification and typing of M-proteins
US5536382A (en) * 1994-05-23 1996-07-16 Advanced Molecular Systems, Inc. Capillary electrophoresis assay method useful for the determination of constituents of a clinical sample
US5431793A (en) * 1994-07-29 1995-07-11 Beckman Instruments, Inc. Quantitative analysis of glycosylated hemoglobin by immunocappillary electrophoresis

Also Published As

Publication number Publication date
DE69624942D1 (en) 2003-01-02
US5783397A (en) 1998-07-21
CA2239418C (en) 2005-09-20
DE69624942T2 (en) 2003-09-18
EP0876609A1 (en) 1998-11-11
DK0876609T3 (en) 2003-03-17
JP2000502443A (en) 2000-02-29
ES2187685T3 (en) 2003-06-16
EP0876609B1 (en) 2002-11-20
WO1997022000A1 (en) 1997-06-19
JP3603267B2 (en) 2004-12-22

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