CA2250212A1 - Device and method for multiple analyte detection - Google Patents
Device and method for multiple analyte detectionInfo
- Publication number
- CA2250212A1 CA2250212A1 CA002250212A CA2250212A CA2250212A1 CA 2250212 A1 CA2250212 A1 CA 2250212A1 CA 002250212 A CA002250212 A CA 002250212A CA 2250212 A CA2250212 A CA 2250212A CA 2250212 A1 CA2250212 A1 CA 2250212A1
- Authority
- CA
- Canada
- Prior art keywords
- analyte
- detection
- sample
- specific
- chambers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
- G01N33/5304—Reaction vessels, e.g. agglutination plates
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
- C12Q1/08—Quantitative determination using multifield media
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0642—Filling fluids into wells by specific techniques
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/141—Preventing contamination, tampering
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0636—Integrated biosensor, microarrays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
- B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N2035/00099—Characterised by type of test elements
- G01N2035/00148—Test cards, e.g. Biomerieux or McDonnel multiwell test cards
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/973—Simultaneous determination of more than one analyte
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/807—Apparatus included in process claim, e.g. physical support structures
Abstract
The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.
Claims (40)
1. A device for detecting or quantitating a plurality of different analytes in a liquid sample, said device comprising a substrate defining a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of said chambers and said inlet, wherein each of said chambers includes an analyte-specific reagent effective to react with a selected analyte that may be present in such sample, to produce a detectable signal, said substrate providing, for each chamber, detection means for detecting such signal, whereby evacuation of said network, followed by application of the sample to said inlet, is effective to draw sample by vacuum into each of said chambers.
2. The device of claim 1, wherein said channel means includes a single channel to which said detection chambers are connected by said fluid connections.
3. The device of claim 1, wherein said channel means includes a first channel to which a first group of detection chambers are connected by such dead-end fluid connections, and a second channel to which a second group of detection chambers are connected by such dead-end fluid connections.
4. The device of claim 1, wherein said channel means includes an individual channel for each detection chamber, for providing a dead-end fluid connection between said inlet and each detection chamber.
5. The device of claim 1, which further includes a vacuum port connected to said channel means at a site in fluid communication with the sample inlet and detection chambers.
6. The device of claim 5, wherein said vacuum port is connected to said channel means at a site that is downstream of said detection chambers.
7. The device of claim 1, which further includes a non-flowthrough vacuum reservoir in fluid communication with said channel means.
8. The device of claim 1, wherein said detection means includes an optically transparent window associated with each detection chamber, through which such signal can be optically detected.
9. The device of claim 1, wherein the analytes are polynucleotides, and at least two of said detection chambers each contain detection reagents for detecting different polynucleotide analytes.
10. The device of claim 9, wherein the analyte-specific detection reagents include first and second oligonucleotide primers having sequences effective to hybridize to opposite end regions of complementary strands of a selected polynucleotide analyte segment, for amplifying the segment by primer-initiated polymerase chain reaction.
11. The device of claim 10, wherein said analyte-specific detection reagents further include a fluorescer-quencher oligonucleotide capable of hybridizing to the analyte segment in a region downstream of one of the primers, for producing a detectable fluorescent signal when the analyte is present in the sample.
12. The device of claim 9, wherein the analyte-specific detection reagents include first and second oligonucleotides effective to bind to adjacent, contiguous regions of a target sequence in the selected analyte, for oligonucleotide ligation assay detection of the analyte.
13. The device of claim 12, wherein the analyte-specific detection reagents include a second pair of oligonucleotides which are effective to bind to adjacent, contiguous regions complementary to the regions bound by the first pair of oligonucleotides, for amplification of the polynucleotide analyte by ligase chain reaction.
14. The device of claim 9, wherein the analyte-specific deotection reagents include a binding polymer effective to hybridize to a selected sequence in the polynucleotide analyte, and the binding polymer includes a fluorescent dye moiety which produces a detectable signal upon hybridizing to the selected sequence.
15. The device of claim 9, wherein the analyte-specific reagents additionally include an intercalating compound which produces an optically detectable signal upon intercalating a double-stranded polynucleotide.
16. The device of claim 1, wherein the analyte-specific reagents in each chamber include an antibody specific for a selected antigen which may be present in the sample.
17. The device of claim 1, wherein the analyte-specific reagents in each chamber include an antigen for reacting with a selected analyte antibody which may be present in the sample.
18. The device of claim 1, wherein said substrate further includes temperature regulating means for controlling the temperature of each detection chamber.
19. The device of claim 1, wherein said substrate defines at least two such sample-distribution networks.
20. The device of claim 1, wherein the interior of said network is under vacuum.
21. A method for detecting or quantitating one or more of a plurality of analytes in a liquid sample, said method comprising providing a device in accordance with claim 1, wherein the interior of the network is under vacuum, applying a liquid sample to the sample inlet and allowing the sample to be drawn into the network by vacuum action to deliver the sample to the detection chambers, allowing the delivered sample to react with the analyte-specific reagents in each detection chamber under conditions effective to produce a detectable signal in each detection chamber when the selected analyte is present in the sample, and measuring the signals produced in the reaction chambers to determine which selected analytes are present in the sample.
22. The method of claim 21, wherein said channel means includes a single channel to which said detection chambers are connected by said fluid connections.
23. The method of claim 21, wherein said channel means includes a first channel to which a first group of detection chambers are connected by such fluid connections, and a second channel to which a second group of detection chambers are connected.
24. The method of claim 21, wherein said channel means includes an individual channel for each detection chamber, for providing a dead-end fluid connection between said inlet and each detection chamber.
25. The method of claim 21, wherein said device further includes a vacuum port connected to the channel means at a site in fluid communication with the sample inlet and detection chambers, and prior to said applying, said network is evacuated by applying a vacuum to the vacuum port.
26. The method of claim 25, wherein said vacuum port is connected to the channel means at a site that is downstream of said sample inlet and said detection chambers.
27. The method of claim 21, wherein said device further includes a non-flowthrough vacuum reservoir in fluid communication with said channel means.
28. The method of claim 21, wherein the analytes are polynucleotides, and at least two of said detection chambers each contain detection reagents for detecting different polynucleotide analytes.
29. The method of claim 28, wherein the analyte-specific reagents include an oligonucleotide primer pair suitable for amplifying, by polymerase chain reaction, an analyte segment which is flanked by sequences complementary to the primer pair.
30. The method of claim 29, wherein said analyte-specific detection reagents further include a fluorescer-quencher oligonucleotide capable of hybridizing to the analyte segment in a region downstream of one of the primers, for producing a detectable fluorescent signal when the analyte is present in the sample.
31. The method of claim 28, wherein the analyte-specific detection reagents include first and second oligonucleotides effective to bind to adjacent contiguous regions of a target sequence in the selected analyte, for oligonucleotide ligation assay detection of the analyte.
32. The method of claim 31, wherein the analyte-specific detection reagents include a second pair of oligonucleotides which are effective to bind to adjacent, contiguous regions complementary to the regions bound by the first pair of oligonucleotides, for amplification of the polynucleotide analyte by ligase chain reaction.
33. The method of claim 28, wherein the analyte-specific detection reagents include a binding polymer effective to hybridize to a selected sequence in the polynucleotide analyte, and the binding polymer includes a fluorescent dye moiety which produces a detectable signal upon hybridizing to the selected sequence.
34. The method of claim 28, wherein the analyte-specific reagents additionally include an intercalating compound which produces an optically detectable signal upon intercalating a double-stranded polynucleotide.
35. The method of claim 21, wherein the analyte-specific reagents in each chamber include an antibody specific for a selected antigen which may be present in the sample.
36. The method of claim 21, wherein the analyte-specific reagents in each chamber include an antigen for reacting with a selected analyte antibody which may be present in the sample.
37. The method of claim 21, wherein said substrate further includes temperature regulating means for controlling the temperature of each detection chamber.
38. The method of claim 21, wherein said substrate defines at least two such sample-distribution networks.
39. The method of claim 21, wherein said providing includes filling said network with carbon dioxide gas, prior to placing said network under vacuum.
40. The method of claim 21, wherein the analyte is a drug candidate, and the detection reagents include a drug target.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US1471296P | 1996-04-03 | 1996-04-03 | |
US60/014712 | 1996-04-03 | ||
PCT/US1997/005458 WO1997036681A1 (en) | 1996-04-03 | 1997-04-02 | Device and method for multiple analyte detection |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2250212A1 true CA2250212A1 (en) | 1997-10-09 |
CA2250212C CA2250212C (en) | 2010-02-09 |
Family
ID=21767215
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2250212A Expired - Fee Related CA2250212C (en) | 1996-04-03 | 1997-04-02 | Device and method for multiple analyte detection |
Country Status (7)
Country | Link |
---|---|
US (2) | US6126899A (en) |
EP (1) | EP0889751B1 (en) |
JP (3) | JP4912517B2 (en) |
AU (1) | AU706862B2 (en) |
CA (1) | CA2250212C (en) |
DE (1) | DE69700499T2 (en) |
WO (1) | WO1997036681A1 (en) |
Families Citing this family (277)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7560273B2 (en) | 2002-07-23 | 2009-07-14 | Applied Biosystems, Llc | Slip cover for heated platen assembly |
US6825047B1 (en) * | 1996-04-03 | 2004-11-30 | Applera Corporation | Device and method for multiple analyte detection |
US7244622B2 (en) * | 1996-04-03 | 2007-07-17 | Applera Corporation | Device and method for multiple analyte detection |
US7235406B1 (en) * | 1996-04-03 | 2007-06-26 | Applera Corporation | Nucleic acid analysis device |
US5779868A (en) * | 1996-06-28 | 1998-07-14 | Caliper Technologies Corporation | Electropipettor and compensation means for electrophoretic bias |
EP0909385B1 (en) | 1996-06-28 | 2008-09-10 | Caliper Life Sciences, Inc. | Method of transporting fluid samples within a microfluidic channel |
FR2762092B1 (en) * | 1997-04-15 | 1999-05-28 | Bio Merieux | METHOD AND DEVICE FOR FILLING AN ANALYSIS CARD WITH A LIQUID MEDIUM |
US6143496A (en) * | 1997-04-17 | 2000-11-07 | Cytonix Corporation | Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly |
US7033474B1 (en) * | 1997-04-25 | 2006-04-25 | Caliper Life Sciences, Inc. | Microfluidic devices incorporating improved channel geometries |
US6300138B1 (en) * | 1997-08-01 | 2001-10-09 | Qualigen, Inc. | Methods for conducting tests |
US6391005B1 (en) | 1998-03-30 | 2002-05-21 | Agilent Technologies, Inc. | Apparatus and method for penetration with shaft having a sensor for sensing penetration depth |
EP1077770B1 (en) | 1998-05-13 | 2005-06-08 | Bayer Corporation | Sample introduction device |
CA2320296A1 (en) | 1998-05-18 | 1999-11-25 | University Of Washington | Liquid analysis cartridge |
US6830729B1 (en) | 1998-05-18 | 2004-12-14 | University Of Washington | Sample analysis instrument |
US6761816B1 (en) | 1998-06-23 | 2004-07-13 | Clinical Micro Systems, Inc. | Printed circuit boards with monolayers and capture ligands |
US6576478B1 (en) | 1998-07-14 | 2003-06-10 | Zyomyx, Inc. | Microdevices for high-throughput screening of biomolecules |
US6897073B2 (en) | 1998-07-14 | 2005-05-24 | Zyomyx, Inc. | Non-specific binding resistant protein arrays and methods for making the same |
US6682942B1 (en) | 1998-07-14 | 2004-01-27 | Zyomyx, Inc. | Microdevices for screening biomolecules |
US6406921B1 (en) | 1998-07-14 | 2002-06-18 | Zyomyx, Incorporated | Protein arrays for high-throughput screening |
FR2790685B1 (en) * | 1999-03-09 | 2001-05-11 | Biomerieux Sa | ANALYSIS CARD WHICH FILLING IS ASSOCIATED WITH AT LEAST ONE BUFFER VOLUME |
FR2790686B3 (en) * | 1999-03-09 | 2001-05-11 | Biomerieux Sa | ANALYSIS CARD WHICH FILLING IS ASSOCIATED WITH AT LEAST ONE BUFFER VOLUME |
FR2790684B1 (en) | 1999-03-09 | 2001-05-11 | Biomerieux Sa | APPARATUS FOR CAPILLARITY TRANSFER OF LIQUIDS |
US6942771B1 (en) * | 1999-04-21 | 2005-09-13 | Clinical Micro Sensors, Inc. | Microfluidic systems in the electrochemical detection of target analytes |
US7157056B2 (en) | 1999-05-12 | 2007-01-02 | Bayer Corporation | Sample introduction device |
IL147227A0 (en) * | 1999-07-02 | 2002-08-14 | Clondiag Chip Tech Gmbh | Microchip matrix device for duplicating and characterizing nucleic acids |
DE19931901A1 (en) * | 1999-07-08 | 2001-01-11 | Basf Ag | Method and device for parallel analysis of colloidal particles with field flow fractionation |
WO2001006253A2 (en) * | 1999-07-16 | 2001-01-25 | Board Of Regents, The University Of Texas System | Detection system based on an analyte reactive particle |
EP1813683A1 (en) | 1999-07-16 | 2007-08-01 | Applera Corporation | Method for polymerase chain reaction in a microfluidic device |
FR2796571B1 (en) * | 1999-07-19 | 2001-09-14 | Bio Merieux | ANALYSIS DEVICE WITH A BIOPUCE |
US7267457B2 (en) * | 1999-09-24 | 2007-09-11 | Cao Group, Inc. | Method for detecting forensic evidence |
US6954270B2 (en) * | 2002-12-20 | 2005-10-11 | Cao Group, Inc. | Method for detecting forensic evidence |
DE50001774D1 (en) | 1999-09-29 | 2003-05-22 | Tecan Trading Ag Maennedorf | Thermal cycler and lifting element for microtiter plate |
GB9922971D0 (en) | 1999-09-29 | 1999-12-01 | Secr Defence | Reaction system |
EP1369699A1 (en) * | 1999-10-15 | 2003-12-10 | PE Corporation (NY) | System and method for filling a substrate with a liquid sample |
US6272939B1 (en) * | 1999-10-15 | 2001-08-14 | Applera Corporation | System and method for filling a substrate with a liquid sample |
US7260248B2 (en) * | 1999-12-15 | 2007-08-21 | Medispectra, Inc. | Image processing using measures of similarity |
US7169355B1 (en) | 2000-02-02 | 2007-01-30 | Applera Corporation | Apparatus and method for ejecting sample well trays |
US6690467B1 (en) | 2000-05-05 | 2004-02-10 | Pe Corporation | Optical system and method for optically analyzing light from a sample |
KR100505803B1 (en) * | 2000-05-26 | 2005-08-04 | 마츠시타 덴끼 산교 가부시키가이샤 | Biosensor |
US6540675B2 (en) | 2000-06-27 | 2003-04-01 | Rosedale Medical, Inc. | Analyte monitor |
US6719949B1 (en) | 2000-06-29 | 2004-04-13 | Applera Corporation | Apparatus and method for transporting sample well trays |
US6563581B1 (en) | 2000-07-14 | 2003-05-13 | Applera Corporation | Scanning system and method for scanning a plurality of samples |
FR2812306B1 (en) * | 2000-07-28 | 2005-01-14 | Gabriel Festoc | POLYMERSIS CHAIN AMPLIFICATION SYSTEM OF TARGET NUCLEIC SEQUENCES |
US7216660B2 (en) * | 2000-11-02 | 2007-05-15 | Princeton University | Method and device for controlling liquid flow on the surface of a microfluidic chip |
US8641644B2 (en) | 2000-11-21 | 2014-02-04 | Sanofi-Aventis Deutschland Gmbh | Blood testing apparatus having a rotatable cartridge with multiple lancing elements and testing means |
US6770434B2 (en) * | 2000-12-29 | 2004-08-03 | The Provost, Fellows And Scholars Of The College Of The Holy & Undivided Trinity Of Queen Elizabeth Near Dublin | Biological assay method |
WO2002077152A1 (en) * | 2001-03-28 | 2002-10-03 | Genetech Biotechnology (Shanghai) Company Limited | Device and method for detection of multiple analytes |
GB0110476D0 (en) | 2001-04-30 | 2001-06-20 | Secr Defence | Reagent delivery system |
US20020168292A1 (en) * | 2001-05-14 | 2002-11-14 | Whisenhunt Donald Wayne | Systems and methods for the high throughput preparation and analysis of chemical reactions |
US9795747B2 (en) | 2010-06-02 | 2017-10-24 | Sanofi-Aventis Deutschland Gmbh | Methods and apparatus for lancet actuation |
US8337419B2 (en) | 2002-04-19 | 2012-12-25 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
US9226699B2 (en) | 2002-04-19 | 2016-01-05 | Sanofi-Aventis Deutschland Gmbh | Body fluid sampling module with a continuous compression tissue interface surface |
US7025774B2 (en) | 2001-06-12 | 2006-04-11 | Pelikan Technologies, Inc. | Tissue penetration device |
US7981056B2 (en) | 2002-04-19 | 2011-07-19 | Pelikan Technologies, Inc. | Methods and apparatus for lancet actuation |
DE60238119D1 (en) | 2001-06-12 | 2010-12-09 | Pelikan Technologies Inc | ELECTRIC ACTUATOR ELEMENT FOR A LANZETTE |
US7316700B2 (en) | 2001-06-12 | 2008-01-08 | Pelikan Technologies, Inc. | Self optimizing lancing device with adaptation means to temporal variations in cutaneous properties |
US7749174B2 (en) | 2001-06-12 | 2010-07-06 | Pelikan Technologies, Inc. | Method and apparatus for lancet launching device intergrated onto a blood-sampling cartridge |
US9427532B2 (en) | 2001-06-12 | 2016-08-30 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
US6514750B2 (en) * | 2001-07-03 | 2003-02-04 | Pe Corporation (Ny) | PCR sample handling device |
US6682702B2 (en) * | 2001-08-24 | 2004-01-27 | Agilent Technologies, Inc. | Apparatus and method for simultaneously conducting multiple chemical reactions |
WO2003023395A1 (en) * | 2001-09-12 | 2003-03-20 | Microbar Systems, Inc. | Enhanced detection of metal plating additives |
US6744502B2 (en) | 2001-09-28 | 2004-06-01 | Pe Corporation (Ny) | Shaped illumination geometry and intensity using a diffractive optical element |
US7338760B2 (en) | 2001-10-26 | 2008-03-04 | Ntu Ventures Private Limited | Sample preparation integrated chip |
US20030138819A1 (en) * | 2001-10-26 | 2003-07-24 | Haiqing Gong | Method for detecting disease |
WO2003048295A1 (en) | 2001-11-30 | 2003-06-12 | Fluidigm Corporation | Microfluidic device and methods of using same |
US7691333B2 (en) | 2001-11-30 | 2010-04-06 | Fluidigm Corporation | Microfluidic device and methods of using same |
US6677151B2 (en) | 2002-01-30 | 2004-01-13 | Applera Corporation | Device and method for thermal cycling |
US7004928B2 (en) | 2002-02-08 | 2006-02-28 | Rosedale Medical, Inc. | Autonomous, ambulatory analyte monitor or drug delivery device |
US7892183B2 (en) | 2002-04-19 | 2011-02-22 | Pelikan Technologies, Inc. | Method and apparatus for body fluid sampling and analyte sensing |
US9314194B2 (en) | 2002-04-19 | 2016-04-19 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
US7491178B2 (en) | 2002-04-19 | 2009-02-17 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US8372016B2 (en) | 2002-04-19 | 2013-02-12 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for body fluid sampling and analyte sensing |
US7901362B2 (en) | 2002-04-19 | 2011-03-08 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US7547287B2 (en) | 2002-04-19 | 2009-06-16 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US7976476B2 (en) | 2002-04-19 | 2011-07-12 | Pelikan Technologies, Inc. | Device and method for variable speed lancet |
US7297122B2 (en) | 2002-04-19 | 2007-11-20 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US7909778B2 (en) | 2002-04-19 | 2011-03-22 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US8579831B2 (en) | 2002-04-19 | 2013-11-12 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for penetrating tissue |
US7175642B2 (en) | 2002-04-19 | 2007-02-13 | Pelikan Technologies, Inc. | Methods and apparatus for lancet actuation |
US9795334B2 (en) | 2002-04-19 | 2017-10-24 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for penetrating tissue |
US8702624B2 (en) | 2006-09-29 | 2014-04-22 | Sanofi-Aventis Deutschland Gmbh | Analyte measurement device with a single shot actuator |
US8784335B2 (en) | 2002-04-19 | 2014-07-22 | Sanofi-Aventis Deutschland Gmbh | Body fluid sampling device with a capacitive sensor |
US7232451B2 (en) | 2002-04-19 | 2007-06-19 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US8221334B2 (en) | 2002-04-19 | 2012-07-17 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for penetrating tissue |
US7331931B2 (en) | 2002-04-19 | 2008-02-19 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US7674232B2 (en) | 2002-04-19 | 2010-03-09 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US7229458B2 (en) | 2002-04-19 | 2007-06-12 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
US9248267B2 (en) | 2002-04-19 | 2016-02-02 | Sanofi-Aventis Deustchland Gmbh | Tissue penetration device |
US7713214B2 (en) | 2002-04-19 | 2010-05-11 | Pelikan Technologies, Inc. | Method and apparatus for a multi-use body fluid sampling device with optical analyte sensing |
US8267870B2 (en) | 2002-04-19 | 2012-09-18 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for body fluid sampling with hybrid actuation |
US8360992B2 (en) | 2002-04-19 | 2013-01-29 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for penetrating tissue |
US20030207441A1 (en) * | 2002-05-01 | 2003-11-06 | Eyster Curt R. | Devices and methods for analyte concentration determination |
US9157860B2 (en) | 2002-05-16 | 2015-10-13 | Applied Biosystems, Llc | Achromatic lens array |
US6982166B2 (en) | 2002-05-16 | 2006-01-03 | Applera Corporation | Lens assembly for biological testing |
US7560239B2 (en) * | 2002-06-04 | 2009-07-14 | Lin-Zhi International Inc. | Homogeneous enzyme immunoassay for simultaneous detection of multiple analytes |
US7459696B2 (en) * | 2003-04-18 | 2008-12-02 | Schomacker Kevin T | Methods and apparatus for calibrating spectral data |
US7083974B2 (en) | 2002-07-12 | 2006-08-01 | Applera Corporation | Rotatable sample disk and method of loading a sample disk |
US7452712B2 (en) | 2002-07-30 | 2008-11-18 | Applied Biosystems Inc. | Sample block apparatus and method of maintaining a microcard on a sample block |
US7905134B2 (en) | 2002-08-06 | 2011-03-15 | The Regents Of The University Of California | Biomarker normalization |
WO2004017050A1 (en) * | 2002-08-06 | 2004-02-26 | The Regents Of The University Of California | Tear film osmometry |
US7810380B2 (en) | 2003-03-25 | 2010-10-12 | Tearlab Research, Inc. | Systems and methods for collecting tear film and measuring tear film osmolarity |
US7534601B2 (en) * | 2002-08-27 | 2009-05-19 | Vanderbilt University | Capillary perfused bioreactors with multiple chambers |
CN1678729A (en) * | 2002-08-27 | 2005-10-05 | 范德比尔特大学 | Capillary perfusion bioreactors with multiple chambers |
US7790443B2 (en) * | 2002-08-27 | 2010-09-07 | Vanderbilt University | Bioreactors with substance injection capacity |
DE10245435B4 (en) * | 2002-09-27 | 2006-03-16 | Micronas Gmbh | Device for detecting at least one ligand contained in a sample to be examined |
US7932098B2 (en) * | 2002-10-31 | 2011-04-26 | Hewlett-Packard Development Company, L.P. | Microfluidic system utilizing thin-film layers to route fluid |
KR100858080B1 (en) * | 2002-11-12 | 2008-09-10 | 삼성전자주식회사 | A method for detecting a PCR product by measuring a electrical signal |
EP1594975A4 (en) | 2002-12-04 | 2006-08-02 | Applera Corp | Multiplex amplification of polynucleotides |
EP1585594A1 (en) * | 2002-12-11 | 2005-10-19 | Inverness Medical Switzerland GmbH | Micro-fluidic structure, method and apparatus for its production, and use thereof |
US8574895B2 (en) | 2002-12-30 | 2013-11-05 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus using optical techniques to measure analyte levels |
US7338637B2 (en) * | 2003-01-31 | 2008-03-04 | Hewlett-Packard Development Company, L.P. | Microfluidic device with thin-film electronic devices |
US7041481B2 (en) | 2003-03-14 | 2006-05-09 | The Regents Of The University Of California | Chemical amplification based on fluid partitioning |
EP2390352A1 (en) | 2003-03-18 | 2011-11-30 | Quantum Genetics Ireland Limited | Systems and methods for improving protein and milk production of dairy herds |
US7052652B2 (en) | 2003-03-24 | 2006-05-30 | Rosedale Medical, Inc. | Analyte concentration detection devices and methods |
US7476363B2 (en) | 2003-04-03 | 2009-01-13 | Fluidigm Corporation | Microfluidic devices and methods of using same |
US7604965B2 (en) | 2003-04-03 | 2009-10-20 | Fluidigm Corporation | Thermal reaction device and method for using the same |
US8828663B2 (en) | 2005-03-18 | 2014-09-09 | Fluidigm Corporation | Thermal reaction device and method for using the same |
EP2340890B1 (en) | 2003-04-03 | 2016-10-19 | Fluidigm Corporation | Method of performimg digital PCR |
US20050145496A1 (en) | 2003-04-03 | 2005-07-07 | Federico Goodsaid | Thermal reaction device and method for using the same |
JP4705035B2 (en) * | 2003-05-23 | 2011-06-22 | バイオ−ラッド ラボラトリーズ,インコーポレイティド | Localized temperature control for spatial arrangement of reaction medium |
US8262614B2 (en) | 2003-05-30 | 2012-09-11 | Pelikan Technologies, Inc. | Method and apparatus for fluid injection |
US7850621B2 (en) | 2003-06-06 | 2010-12-14 | Pelikan Technologies, Inc. | Method and apparatus for body fluid sampling and analyte sensing |
AU2003241850A1 (en) * | 2003-06-07 | 2005-01-04 | Genomictree Inc. | Multi-hybridization set for dna microarray related assay |
WO2006001797A1 (en) | 2004-06-14 | 2006-01-05 | Pelikan Technologies, Inc. | Low pain penetrating |
US20040258563A1 (en) | 2003-06-23 | 2004-12-23 | Applera Corporation | Caps for sample wells and microcards for biological materials |
US6883851B2 (en) * | 2003-09-16 | 2005-04-26 | Honda Motor Company, Ltd. | Cargo carrying system having multiple closure members |
WO2005028110A2 (en) * | 2003-09-19 | 2005-03-31 | Applera Corporation | Microplates useful for conducting thermocycled nucleotide amplification |
US20050232818A1 (en) * | 2003-09-19 | 2005-10-20 | Donald Sandell | Single sheet seal applicator and cartridge |
US20060013984A1 (en) * | 2003-09-19 | 2006-01-19 | Donald Sandell | Film preparation for seal applicator |
US20060011305A1 (en) * | 2003-09-19 | 2006-01-19 | Donald Sandell | Automated seal applicator |
US20070014694A1 (en) * | 2003-09-19 | 2007-01-18 | Beard Nigel P | High density plate filler |
US20050232821A1 (en) * | 2003-09-19 | 2005-10-20 | Carrillo Albert L | High density plate filler |
US7570443B2 (en) | 2003-09-19 | 2009-08-04 | Applied Biosystems, Llc | Optical camera alignment |
US7407630B2 (en) | 2003-09-19 | 2008-08-05 | Applera Corporation | High density plate filler |
US7695688B2 (en) * | 2003-09-19 | 2010-04-13 | Applied Biosystems, Llc | High density plate filler |
US9492820B2 (en) | 2003-09-19 | 2016-11-15 | Applied Biosystems, Llc | High density plate filler |
US20060029948A1 (en) * | 2003-09-19 | 2006-02-09 | Gary Lim | Sealing cover and dye compatibility selection |
US7998435B2 (en) | 2003-09-19 | 2011-08-16 | Life Technologies Corporation | High density plate filler |
US20050226780A1 (en) * | 2003-09-19 | 2005-10-13 | Donald Sandell | Manual seal applicator |
US8277760B2 (en) | 2003-09-19 | 2012-10-02 | Applied Biosystems, Llc | High density plate filler |
WO2005033659A2 (en) | 2003-09-29 | 2005-04-14 | Pelikan Technologies, Inc. | Method and apparatus for an improved sample capture device |
EP1680014A4 (en) | 2003-10-14 | 2009-01-21 | Pelikan Technologies Inc | Method and apparatus for a variable user interface |
EP1694812A4 (en) | 2003-12-16 | 2008-11-12 | Canon Kk | Biologically active substance transfer sheet, cell culture kit constituted of cell culture plate and biologically active substance transfer sheet, producing method thereof and method for screening cell culture conditions utilizing the same |
US7822454B1 (en) | 2005-01-03 | 2010-10-26 | Pelikan Technologies, Inc. | Fluid sampling device with improved analyte detecting member configuration |
US8668656B2 (en) | 2003-12-31 | 2014-03-11 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for improving fluidic flow and sample capture |
EP1703981A1 (en) * | 2004-01-12 | 2006-09-27 | Applera Corporation | Method and device for detection of nucleic acid sequences |
US20050164373A1 (en) * | 2004-01-22 | 2005-07-28 | Oldham Mark F. | Diffusion-aided loading system for microfluidic devices |
NZ549384A (en) | 2004-02-19 | 2008-08-29 | Univ Alberta | Leptin promoter polymorphisms and uses thereof |
US20050255485A1 (en) * | 2004-05-14 | 2005-11-17 | Livak Kenneth J | Detection of gene duplications |
WO2006011062A2 (en) | 2004-05-20 | 2006-02-02 | Albatros Technologies Gmbh & Co. Kg | Printable hydrogel for biosensors |
US9775553B2 (en) | 2004-06-03 | 2017-10-03 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for a fluid sampling device |
EP1765194A4 (en) | 2004-06-03 | 2010-09-29 | Pelikan Technologies Inc | Method and apparatus for a fluid sampling device |
US8563328B2 (en) * | 2004-07-26 | 2013-10-22 | University Of Louisville Research Foundation, Inc. | Fiber-optic biosensor and biosensing methods |
FR2874385B1 (en) * | 2004-08-18 | 2006-12-08 | Biopep Sa | METHOD AND DEVICE FOR MEASURING ENZYMA ACTIVITY IN A BIOLOGICAL FLUID |
US20110038758A1 (en) * | 2004-11-22 | 2011-02-17 | Nissui Pharmaceutical Co., Ltd. | Microchip |
US8084260B2 (en) * | 2004-11-24 | 2011-12-27 | Applied Biosystems, Llc | Spectral calibration method and system for multiple instruments |
US8695355B2 (en) * | 2004-12-08 | 2014-04-15 | California Institute Of Technology | Thermal management techniques, apparatus and methods for use in microfluidic devices |
US8652831B2 (en) | 2004-12-30 | 2014-02-18 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for analyte measurement test time |
JP2006223126A (en) * | 2005-02-15 | 2006-08-31 | Shimadzu Corp | Reaction vessel, method and apparatus for detecting genetic polymorphism and method and apparatus for diagnosis |
WO2006088976A2 (en) * | 2005-02-16 | 2006-08-24 | University Of Louisville Research Foundation, Inc. | Fiber-optic biosensor and biosensing methods |
US8008066B2 (en) | 2005-03-10 | 2011-08-30 | Gen-Probe Incorporated | System for performing multi-formatted assays |
EP1707267A1 (en) * | 2005-03-30 | 2006-10-04 | F. Hoffman-la Roche AG | Device having a self sealing fluid port |
US20060246493A1 (en) | 2005-04-04 | 2006-11-02 | Caliper Life Sciences, Inc. | Method and apparatus for use in temperature controlled processing of microfluidic samples |
EP1885839B1 (en) * | 2005-04-26 | 2018-08-08 | Life Technologies Corporation | Systems and methods for multiple analyte detection |
US20070026426A1 (en) * | 2005-04-26 | 2007-02-01 | Applera Corporation | System for genetic surveillance and analysis |
US20060281187A1 (en) | 2005-06-13 | 2006-12-14 | Rosedale Medical, Inc. | Analyte detection devices and methods with hematocrit/volume correction and feedback control |
US20070175768A1 (en) * | 2005-06-30 | 2007-08-02 | Applera Corporation | Microfluidic systems including porous polymer electrodes |
EP1907832A1 (en) * | 2005-06-30 | 2008-04-09 | Applera Corporation | Porous composite electrode comprising conductive polymer |
EP3461406A1 (en) | 2005-09-30 | 2019-04-03 | Intuity Medical, Inc. | Multi-site body fluid sampling and analysis cartridge |
US8801631B2 (en) | 2005-09-30 | 2014-08-12 | Intuity Medical, Inc. | Devices and methods for facilitating fluid transport |
US8831887B2 (en) * | 2005-10-12 | 2014-09-09 | The Research Foundation For The State University Of New York | Absolute PCR quantification |
WO2007061305A2 (en) * | 2005-11-22 | 2007-05-31 | Stichting Top Institute Food And Nutrition | Sampling device for in vivo sampling of liquids from the gastrointestinal tract, process for the production thereof and mould or mask for use in the production process |
US8900828B2 (en) * | 2006-05-01 | 2014-12-02 | Cepheid | Methods and apparatus for sequential amplification reactions |
US20070280857A1 (en) * | 2006-06-02 | 2007-12-06 | Applera Corporation | Devices and Methods for Positioning Dried Reagent In Microfluidic Devices |
US7759062B2 (en) * | 2006-06-09 | 2010-07-20 | Third Wave Technologies, Inc. | T-structure invasive cleavage assays, consistent nucleic acid dispensing, and low level target nucleic acid detection |
WO2008002562A2 (en) * | 2006-06-26 | 2008-01-03 | Applera Corporation | Compressible transparent sealing for open microplates |
WO2008045251A2 (en) | 2006-10-04 | 2008-04-17 | Third Wave Technologies, Inc. | Snap-back primers and detectable hairpin structures |
US20080245740A1 (en) * | 2007-01-29 | 2008-10-09 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Fluidic methods |
US9550184B2 (en) * | 2007-02-05 | 2017-01-24 | Shimadzu Corporation | Reactor plate and reaction processing method |
US20100086990A1 (en) * | 2007-03-02 | 2010-04-08 | Corbett Research Pty Ltd | Apparatus and method for nucleic acid amplification |
JP5282088B2 (en) * | 2007-06-21 | 2013-09-04 | ジェン−プロウブ インコーポレイテッド | Equipment and containers used for processing execution |
JP2009042103A (en) * | 2007-08-09 | 2009-02-26 | Sony Corp | Base, reaction processor using it and reaction control method |
US20090055243A1 (en) | 2007-08-21 | 2009-02-26 | Jayson Lee Lusk | Systems and methods for predicting a livestock marketing method |
US20090081768A1 (en) * | 2007-09-21 | 2009-03-26 | Applera Corporation | Devices and Methods for Thermally Isolating Chambers of an Assay Card |
JP5251882B2 (en) * | 2007-10-26 | 2013-07-31 | 凸版印刷株式会社 | Reaction chip and reaction method, temperature control mechanism for gene processing apparatus and gene processing apparatus |
US9211537B2 (en) * | 2007-11-07 | 2015-12-15 | The University Of British Columbia | Microfluidic device and method of using same |
JP5441142B2 (en) * | 2007-11-26 | 2014-03-12 | 国立大学法人 東京大学 | Microfluidic planar lipid bilayer array and analytical method using the planar lipid bilayer membrane |
KR101362905B1 (en) * | 2007-11-30 | 2014-02-19 | (주)바이오니아 | The micro-chamber plate, manufacturing method thereof |
AU2008339105A1 (en) * | 2007-12-17 | 2009-06-25 | Hai-Qing Gong | Microfluidic device |
JP4556194B2 (en) * | 2008-02-01 | 2010-10-06 | セイコーエプソン株式会社 | Biological sample reaction method |
KR100969667B1 (en) * | 2008-03-24 | 2010-07-14 | 디지탈 지노믹스(주) | Method for detecting biomolecules electrically and biochip provided with therefor |
US8834797B2 (en) | 2008-04-04 | 2014-09-16 | Life Technologies Corporation | Scanning system and method for imaging and sequencing |
EP2265324B1 (en) | 2008-04-11 | 2015-01-28 | Sanofi-Aventis Deutschland GmbH | Integrated analyte measurement system |
JP5816080B2 (en) | 2008-05-30 | 2015-11-17 | インテュイティ メディカル インコーポレイテッド | Body fluid collection device and collection site interface |
ES2907152T3 (en) | 2008-06-06 | 2022-04-22 | Intuity Medical Inc | Blood glucose meter and method of operation |
US9636051B2 (en) | 2008-06-06 | 2017-05-02 | Intuity Medical, Inc. | Detection meter and mode of operation |
US9180453B2 (en) * | 2008-08-15 | 2015-11-10 | University Of Washington | Method and apparatus for the discretization and manipulation of sample volumes |
US20100055733A1 (en) * | 2008-09-04 | 2010-03-04 | Lutolf Matthias P | Manufacture and uses of reactive microcontact printing of biomolecules on soft hydrogels |
US8951939B2 (en) | 2011-07-12 | 2015-02-10 | Bio-Rad Laboratories, Inc. | Digital assays with multiplexed detection of two or more targets in the same optical channel |
US9598725B2 (en) | 2010-03-02 | 2017-03-21 | Bio-Rad Laboratories, Inc. | Emulsion chemistry for encapsulated droplets |
US9156010B2 (en) | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
US9764322B2 (en) | 2008-09-23 | 2017-09-19 | Bio-Rad Laboratories, Inc. | System for generating droplets with pressure monitoring |
US9417190B2 (en) | 2008-09-23 | 2016-08-16 | Bio-Rad Laboratories, Inc. | Calibrations and controls for droplet-based assays |
US8709762B2 (en) | 2010-03-02 | 2014-04-29 | Bio-Rad Laboratories, Inc. | System for hot-start amplification via a multiple emulsion |
US8633015B2 (en) | 2008-09-23 | 2014-01-21 | Bio-Rad Laboratories, Inc. | Flow-based thermocycling system with thermoelectric cooler |
US20230372935A1 (en) * | 2008-09-23 | 2023-11-23 | Bio-Rad Laboratories, Inc. | Partition-based method of analysis |
US10512910B2 (en) | 2008-09-23 | 2019-12-24 | Bio-Rad Laboratories, Inc. | Droplet-based analysis method |
US9132394B2 (en) | 2008-09-23 | 2015-09-15 | Bio-Rad Laboratories, Inc. | System for detection of spaced droplets |
US9492797B2 (en) | 2008-09-23 | 2016-11-15 | Bio-Rad Laboratories, Inc. | System for detection of spaced droplets |
US11130128B2 (en) | 2008-09-23 | 2021-09-28 | Bio-Rad Laboratories, Inc. | Detection method for a target nucleic acid |
US20100119454A1 (en) * | 2008-11-03 | 2010-05-13 | Ping Shen | Use of the conserved Drosophila NPFR1 system for uncovering interacting genes and pathways important in nociception and stress response |
US9375169B2 (en) | 2009-01-30 | 2016-06-28 | Sanofi-Aventis Deutschland Gmbh | Cam drive for managing disposable penetrating member actions with a single motor and motor and control system |
WO2012018486A2 (en) | 2010-07-26 | 2012-02-09 | Seventh Sense Biosystems, Inc. | Rapid delivery and/or receiving of fluids |
WO2010101620A2 (en) | 2009-03-02 | 2010-09-10 | Seventh Sense Biosystems, Inc. | Systems and methods for creating and using suction blisters or other pooled regions of fluid within the skin |
US20120277629A1 (en) | 2011-04-29 | 2012-11-01 | Seventh Sense Biosystems, Inc. | Systems and methods for collection and/or manipulation of blood spots or other bodily fluids |
US9447461B2 (en) | 2009-03-24 | 2016-09-20 | California Institute Of Technology | Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes |
US10196700B2 (en) | 2009-03-24 | 2019-02-05 | University Of Chicago | Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes |
EP2412020B1 (en) * | 2009-03-24 | 2020-09-30 | University Of Chicago | Slip chip device and methods |
JP5881936B2 (en) * | 2009-04-20 | 2016-03-09 | ソニー株式会社 | Sample solution introduction kit and sample solution injector |
JP5198369B2 (en) * | 2009-06-19 | 2013-05-15 | 国立大学法人 東京大学 | Current measurement device using artificial lipid bilayer membrane |
EP2473618B1 (en) | 2009-09-02 | 2015-03-04 | Bio-Rad Laboratories, Inc. | System for mixing fluids by coalescence of multiple emulsions |
WO2011053787A2 (en) * | 2009-10-30 | 2011-05-05 | Seventh Sense Biosystems, Inc. | Systems and methods for application to skin and control of actuation, delivery and/or perception thereof |
EP3106871B1 (en) | 2009-11-30 | 2021-10-27 | Intuity Medical, Inc. | A method of verifying the accuracy of the operation of an analyte monitoring device |
WO2011094573A1 (en) | 2010-01-28 | 2011-08-04 | Seventh Sense Biosystems, Inc. | Monitoring or feedback systems and methods |
JP5218443B2 (en) | 2010-02-10 | 2013-06-26 | ソニー株式会社 | Microchip and manufacturing method of microchip |
CA2767182C (en) | 2010-03-25 | 2020-03-24 | Bio-Rad Laboratories, Inc. | Droplet generation for droplet-based assays |
JP6155419B2 (en) | 2010-03-25 | 2017-07-05 | バイオ−ラッド・ラボラトリーズ・インコーポレーテッド | Droplet transport system for detection |
CA2767113A1 (en) | 2010-03-25 | 2011-09-29 | Bio-Rad Laboratories, Inc. | Detection system for droplet-based assays |
US8965476B2 (en) | 2010-04-16 | 2015-02-24 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
WO2011163347A2 (en) | 2010-06-23 | 2011-12-29 | Seventh Sense Biosystems, Inc. | Sampling devices and methods involving relatively little pain |
EP2584964B1 (en) | 2010-06-25 | 2021-08-04 | Intuity Medical, Inc. | Analyte monitoring devices |
WO2012009613A1 (en) | 2010-07-16 | 2012-01-19 | Seventh Sense Biosystems, Inc. | Low-pressure environment for fluid transfer devices |
CN103140755B (en) | 2010-07-22 | 2016-03-16 | 哈希公司 | For the lab on A Chip of alkalinity analyzing |
US9046507B2 (en) | 2010-07-29 | 2015-06-02 | Gen-Probe Incorporated | Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure |
WO2012021801A2 (en) | 2010-08-13 | 2012-02-16 | Seventh Sense Biosystems, Inc. | Systems and techniques for monitoring subjects |
DE202011110979U1 (en) | 2010-11-01 | 2017-12-04 | Bio-Rad Laboratories, Inc. | System for forming emulsions |
WO2012064802A1 (en) | 2010-11-09 | 2012-05-18 | Seventh Sense Biosystems, Inc. | Systems and interfaces for blood sampling |
US8718948B2 (en) | 2011-02-24 | 2014-05-06 | Gen-Probe Incorporated | Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector |
CA2830443C (en) | 2011-03-18 | 2021-11-16 | Bio-Rad Laboratories, Inc. | Multiplexed digital assays with combinatorial use of signals |
CA2834291A1 (en) | 2011-04-25 | 2012-11-01 | Biorad Laboratories, Inc. | Methods and compositions for nucleic acid analysis |
US20130158468A1 (en) | 2011-12-19 | 2013-06-20 | Seventh Sense Biosystems, Inc. | Delivering and/or receiving material with respect to a subject surface |
EP2701600B1 (en) | 2011-04-29 | 2016-06-08 | Seventh Sense Biosystems, Inc. | Delivering and/or receiving fluids |
WO2012149155A1 (en) | 2011-04-29 | 2012-11-01 | Seventh Sense Biosystems, Inc. | Systems and methods for collecting fluid from a subject |
EP2737089B1 (en) | 2011-07-29 | 2017-09-06 | Bio-rad Laboratories, Inc. | Library characterization by digital assay |
US9782114B2 (en) | 2011-08-03 | 2017-10-10 | Intuity Medical, Inc. | Devices and methods for body fluid sampling and analysis |
CN103946398B (en) | 2011-09-15 | 2019-08-13 | 健能泰格技术公司 | Probe: detection of the antisense probe composition to high specific DNA or RNA |
US9709469B2 (en) * | 2011-11-11 | 2017-07-18 | The Regents Of The University Of California | Valveless microfluidic device |
EP2785460B1 (en) * | 2011-11-29 | 2021-01-27 | Caliper Life Sciences, Inc. | Systems and methods for sampling of amplification products |
US9689029B2 (en) | 2011-12-02 | 2017-06-27 | Caliper Life Sciences, Inc. | Systems and methods for sampling of amplification products |
WO2013155531A2 (en) | 2012-04-13 | 2013-10-17 | Bio-Rad Laboratories, Inc. | Sample holder with a well having a wicking promoter |
US9063121B2 (en) | 2012-05-09 | 2015-06-23 | Stat-Diagnostica & Innovation, S.L. | Plurality of reaction chambers in a test cartridge |
US9180449B2 (en) | 2012-06-12 | 2015-11-10 | Hach Company | Mobile water analysis |
JPWO2014058064A1 (en) * | 2012-10-12 | 2016-09-05 | 学校法人日本大学 | Inspection system and inspection method |
CN103852576B (en) * | 2012-12-03 | 2017-11-28 | 科宝智慧医疗科技(上海)有限公司 | Container for fluid analysis |
USD768872S1 (en) | 2012-12-12 | 2016-10-11 | Hach Company | Cuvette for a water analysis instrument |
JP5708683B2 (en) * | 2013-02-26 | 2015-04-30 | ソニー株式会社 | Microchip and manufacturing method of microchip |
US20160107159A1 (en) * | 2013-05-27 | 2016-04-21 | Star Array Pte Ltd | Microfluidic device and method for controlling fluid flow thereinto |
WO2014205412A1 (en) | 2013-06-21 | 2014-12-24 | Intuity Medical, Inc. | Analyte monitoring system with audible feedback |
CN105431726A (en) | 2013-06-25 | 2016-03-23 | 华盛顿大学商业中心 | Self-digitization of sample volumes |
EP3093665B1 (en) * | 2014-01-10 | 2019-11-27 | Konica Minolta, Inc. | Immunoassay method and immunoassay system |
KR20150101307A (en) * | 2014-02-26 | 2015-09-03 | 삼성전자주식회사 | Microfluidic device |
WO2015175189A1 (en) * | 2014-05-15 | 2015-11-19 | General Electric Company | Microfluidic flow cell assemblies and method of use |
EP3198257B1 (en) | 2014-09-23 | 2020-08-12 | Tearlab Research, Inc. | System for integration of microfluidic tear collection and lateral flow analysis of analytes of interest |
CN107405619B (en) | 2015-01-14 | 2020-03-13 | 彼克斯赛尔医疗科技有限公司 | Disposable cartridge for sample fluid analysis |
US10974246B2 (en) | 2015-06-08 | 2021-04-13 | Japan Science And Technology Agency | High-density micro-chamber array and measurement method using same |
JP6868289B2 (en) | 2015-07-07 | 2021-05-12 | ユニバーシティ オブ ワシントンUniversity of Washington | Systems, methods, and devices for self-digitization of samples |
US10775370B2 (en) | 2015-07-17 | 2020-09-15 | Stat-Diagnostica & Innovation, S.L. | Fluidic system for performing assays |
US10857536B2 (en) | 2016-01-08 | 2020-12-08 | Hewlett-Packard Development Company, L.P. | Polymerase chain reaction device |
US10946379B2 (en) | 2016-01-29 | 2021-03-16 | Hewlett-Packard Development Company, L.P. | Microfluidics system |
JP2019533005A (en) * | 2016-09-06 | 2019-11-14 | デビナ・ダイアグノスティクス・インコーポレイテッドDebina Diagnostics, Inc. | Nanodiamond particles and related apparatus and methods |
US10328147B2 (en) | 2017-03-24 | 2019-06-25 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Herpes simplex virus type-1(HSV-1) vaccine strain VC2 generating an anti-EHV-1 immune response |
US11852605B2 (en) | 2018-06-21 | 2023-12-26 | Trustees Of Dartmouth College | Metal-organic frameworks for electrochemical detection of analytes |
WO2020105628A1 (en) * | 2018-11-21 | 2020-05-28 | 株式会社ヨコオ | Method for detecting target nucleic acid and package for target nucleic acid detection |
US20220062850A1 (en) * | 2018-12-20 | 2022-03-03 | Imec Vzw | Addressable micro-reaction chamber array |
WO2020183938A1 (en) * | 2019-03-08 | 2020-09-17 | 株式会社フコク | Microchannel chip |
CN113924165A (en) * | 2019-06-07 | 2022-01-11 | 通用自动化实验技术公司 | Loading and sealing samples on microfabricated chips |
CN111257571A (en) * | 2020-02-27 | 2020-06-09 | 南京亿科人群健康研究院有限公司 | Kit for rapidly detecting animal hydatid natural infection antibody |
Family Cites Families (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3963355A (en) * | 1972-05-22 | 1976-06-15 | Mcdonnell Douglas Corporation | Process and apparatus for analyzing specimens for the presence of microorganisms therein |
US4038151A (en) * | 1976-07-29 | 1977-07-26 | Mcdonnell Douglas Corporation | Card for use in an automated microbial detection system |
US4704255A (en) * | 1983-07-15 | 1987-11-03 | Pandex Laboratories, Inc. | Assay cartridge |
US5229297A (en) * | 1989-02-03 | 1993-07-20 | Eastman Kodak Company | Containment cuvette for PCR and method of use |
US5252296A (en) * | 1990-05-15 | 1993-10-12 | Chiron Corporation | Method and apparatus for biopolymer synthesis |
US5455175A (en) * | 1990-06-04 | 1995-10-03 | University Of Utah Research Foundation | Rapid thermal cycling device |
US5182082A (en) * | 1991-01-23 | 1993-01-26 | Becton, Dickinson And Company | Multiple aliquot device for distributing a liquid solution into a well |
US5270183A (en) * | 1991-02-08 | 1993-12-14 | Beckman Research Institute Of The City Of Hope | Device and method for the automated cycling of solutions between two or more temperatures |
EP1130116A3 (en) * | 1992-04-06 | 2004-01-14 | Abbott Laboratories | Method and device for detection of nucleic acid using total internal reflectance |
US5498392A (en) * | 1992-05-01 | 1996-03-12 | Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification device and method |
US5304487A (en) * | 1992-05-01 | 1994-04-19 | Trustees Of The University Of Pennsylvania | Fluid handling in mesoscale analytical devices |
US5296375A (en) * | 1992-05-01 | 1994-03-22 | Trustees Of The University Of Pennsylvania | Mesoscale sperm handling devices |
ATE174813T1 (en) * | 1992-05-01 | 1999-01-15 | Univ Pennsylvania | POLYNUCLEOTIDE AMPLIFICATION ANALYSIS USING A MICROFABRICATED DEVICE |
WO1994002636A1 (en) * | 1992-07-28 | 1994-02-03 | Hitachi Chemical Company Ltd. | Gene detection system |
JP3305322B2 (en) * | 1992-11-06 | 2002-07-22 | バイオログ,インコーポレーテッド | Liquid and suspension analyzers |
US5591643A (en) * | 1993-09-01 | 1997-01-07 | Abaxis, Inc. | Simplified inlet channels |
US5409665A (en) * | 1993-09-01 | 1995-04-25 | Abaxis, Inc. | Simultaneous cuvette filling with means to isolate cuvettes |
ES2325393T3 (en) * | 1993-10-28 | 2009-09-03 | Houston Advanced Research Center | POROUS FLOW EQUIPMENT THROUGH MICROFABRICATED FOR THE DIFFERENTIATED DETECTION OF UNION REACTIONS. |
JP3383310B2 (en) * | 1994-02-01 | 2003-03-04 | ロバート イー. フィールズ, | Molecular analyzer and method of use |
US5595712A (en) * | 1994-07-25 | 1997-01-21 | E. I. Du Pont De Nemours And Company | Chemical mixing and reaction apparatus |
US6001229A (en) * | 1994-08-01 | 1999-12-14 | Lockheed Martin Energy Systems, Inc. | Apparatus and method for performing microfluidic manipulations for chemical analysis |
US5585069A (en) * | 1994-11-10 | 1996-12-17 | David Sarnoff Research Center, Inc. | Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis |
US5612473A (en) * | 1996-01-16 | 1997-03-18 | Gull Laboratories | Methods, kits and solutions for preparing sample material for nucleic acid amplification |
US5846727A (en) * | 1996-06-06 | 1998-12-08 | Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College | Microsystem for rapid DNA sequencing |
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1997
- 1997-04-02 US US08/831,983 patent/US6126899A/en not_active Expired - Lifetime
- 1997-04-02 EP EP97917816A patent/EP0889751B1/en not_active Expired - Lifetime
- 1997-04-02 WO PCT/US1997/005458 patent/WO1997036681A1/en active IP Right Grant
- 1997-04-02 JP JP53557597A patent/JP4912517B2/en not_active Expired - Lifetime
- 1997-04-02 AU AU26048/97A patent/AU706862B2/en not_active Ceased
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- 1997-04-02 DE DE69700499T patent/DE69700499T2/en not_active Expired - Lifetime
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1998
- 1998-01-22 US US09/012,045 patent/US6124138A/en not_active Expired - Lifetime
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2008
- 2008-09-05 JP JP2008229123A patent/JP2009025313A/en not_active Withdrawn
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2011
- 2011-04-01 JP JP2011082316A patent/JP2011177179A/en not_active Withdrawn
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JP2000508528A (en) | 2000-07-11 |
EP0889751A1 (en) | 1999-01-13 |
JP2011177179A (en) | 2011-09-15 |
US6126899A (en) | 2000-10-03 |
AU2604897A (en) | 1997-10-22 |
CA2250212C (en) | 2010-02-09 |
US6124138A (en) | 2000-09-26 |
DE69700499D1 (en) | 1999-10-14 |
EP0889751B1 (en) | 1999-09-08 |
AU706862B2 (en) | 1999-06-24 |
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