CA2256686A1 - Multicomponent analysis method including the determination of a statistical confidence interval - Google Patents

Multicomponent analysis method including the determination of a statistical confidence interval

Info

Publication number
CA2256686A1
CA2256686A1 CA002256686A CA2256686A CA2256686A1 CA 2256686 A1 CA2256686 A1 CA 2256686A1 CA 002256686 A CA002256686 A CA 002256686A CA 2256686 A CA2256686 A CA 2256686A CA 2256686 A1 CA2256686 A1 CA 2256686A1
Authority
CA
Canada
Prior art keywords
confidence interval
cii
determination
concentration estimate
vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002256686A
Other languages
French (fr)
Other versions
CA2256686C (en
Inventor
Muhammad A. Sharaf
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2256686A1 publication Critical patent/CA2256686A1/en
Application granted granted Critical
Publication of CA2256686C publication Critical patent/CA2256686C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/86—Signal analysis
    • G01N30/8665—Signal analysis for calibrating the measuring apparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/12—Circuits of general importance; Signal processing
    • G01N2201/129—Using chemometrical methods

Abstract

A signal processing method in a processor is provided for performing a multicomponent analysis of a signal resulting from a spectral response of a mixture comprising a plurality of spectrally resolvable molecular species. The method provides both a determination of a concentration estimate and a statistical confidence interval for each species. In the method, a data vector d is received from a multichannel detector, data vector d having a length nc, nc being the number of detector channels being monitored. A calibration matrix K having nc rows and np columns is provided wherein nc is larger than np, np being the number of spectrally resolvable molecular species. Next, a concentration estimate vector c having length np is determined. Finally, a confidence interval CIi for each of the elements of the concentration estimate vector is determined according to the expression CIi = ci ~
(varcovar(cii))1/2Q(cl, nf) where Q is the critical value of a statistical distribution for a given level of confidence, cl, and a given number of degrees of freedom nf where nf = nc-np. The invention further includes a program storage device embodying the method; a DNA sequencing process employing the method; and, an apparatus for carrying out the method.
CA002256686A 1996-06-03 1997-05-15 Multicomponent analysis method including the determination of a statistical confidence interval Expired - Fee Related CA2256686C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/659,115 1996-06-03
US08/659,115 US6015667A (en) 1996-06-03 1996-06-03 Multicomponent analysis method including the determination of a statistical confidence interval
PCT/US1997/008237 WO1997046963A1 (en) 1996-06-03 1997-05-15 Multicomponent analysis method including the determination of a statistical confidence interval

Publications (2)

Publication Number Publication Date
CA2256686A1 true CA2256686A1 (en) 1997-12-11
CA2256686C CA2256686C (en) 2003-01-21

Family

ID=24644103

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002256686A Expired - Fee Related CA2256686C (en) 1996-06-03 1997-05-15 Multicomponent analysis method including the determination of a statistical confidence interval

Country Status (8)

Country Link
US (1) US6015667A (en)
EP (1) EP0897568B1 (en)
JP (1) JP3287361B2 (en)
AT (1) ATE203605T1 (en)
AU (1) AU707113B2 (en)
CA (1) CA2256686C (en)
DE (1) DE69705834T2 (en)
WO (1) WO1997046963A1 (en)

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US6369893B1 (en) 1998-05-19 2002-04-09 Cepheid Multi-channel optical detection system
US6019896A (en) * 1998-03-06 2000-02-01 Molecular Dynamics, Inc. Method for using a quality metric to assess the quality of biochemical separations
DE19810917A1 (en) * 1998-03-13 1999-09-16 Buehler Ag Calibration method used in evaluation of measured spectra
US7321828B2 (en) * 1998-04-13 2008-01-22 Isis Pharmaceuticals, Inc. System of components for preparing oligonucleotides
US20030228597A1 (en) * 1998-04-13 2003-12-11 Cowsert Lex M. Identification of genetic targets for modulation by oligonucleotides and generation of oligonucleotides for gene modulation
US6821402B1 (en) 1998-09-16 2004-11-23 Applera Corporation Spectral calibration of fluorescent polynucleotide separation apparatus
WO2000075636A1 (en) * 1999-06-04 2000-12-14 Kairos Scientific, Inc. Multispectral taxonomic identification
US6738502B1 (en) 1999-06-04 2004-05-18 Kairos Scientific, Inc. Multispectral taxonomic identification
US6480808B1 (en) * 1999-12-22 2002-11-12 General Electric Company Method and apparatus for calculating confidence intervals
US6463391B1 (en) * 1999-12-22 2002-10-08 General Electric Company Method and apparatus for calculating confidence intervals
US6333501B1 (en) 2000-01-27 2001-12-25 Perkin-Elmer Corporation Methods, apparatus, and articles of manufacture for performing spectral calibration
US6418383B1 (en) * 2000-02-11 2002-07-09 Perkinelmer Instruments Llc Method and apparatus for iterative spectral compensation
US6681186B1 (en) 2000-09-08 2004-01-20 Paracel, Inc. System and method for improving the accuracy of DNA sequencing and error probability estimation through application of a mathematical model to the analysis of electropherograms
US6863791B1 (en) 2000-09-11 2005-03-08 Spectrumedix Llc Method for in-situ calibration of electrophoretic analysis systems
US6524803B2 (en) 2000-12-19 2003-02-25 Agilent Technologies, Inc. Deconvolution method and apparatus for analyzing compounds
US6652273B2 (en) * 2002-01-14 2003-11-25 The Procter & Gamble Company Apparatus and method for controlling the temperature of manufacturing equipment
JP3903000B2 (en) 2002-11-14 2007-04-11 アークレイ株式会社 Measuring apparatus, fluorescence measuring apparatus and fluorescence measuring method
JP2004286716A (en) * 2003-03-25 2004-10-14 Institute Of Physical & Chemical Research Target distinguishing method and device by terahertz wave spectroscopic measurement
JP4686451B2 (en) * 2003-04-28 2011-05-25 セルノ・バイオサイエンス・エルエルシー Multidimensional analysis calculation method and system
US6983213B2 (en) * 2003-10-20 2006-01-03 Cerno Bioscience Llc Methods for operating mass spectrometry (MS) instrument systems
US7417726B2 (en) 2003-09-19 2008-08-26 Applied Biosystems Inc. Normalization of data using controls
US20060029948A1 (en) * 2003-09-19 2006-02-09 Gary Lim Sealing cover and dye compatibility selection
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
US7460958B2 (en) * 2004-10-07 2008-12-02 E.I. Du Pont De Nemours And Company Computer-implemented system and method for analyzing mixtures of gases
US8084260B2 (en) * 2004-11-24 2011-12-27 Applied Biosystems, Llc Spectral calibration method and system for multiple instruments
EP1990630B1 (en) * 2006-02-23 2022-08-24 Nikon Corporation Spectrum image processing method, spectrum image processing program, and spectrum imaging system
US8055035B2 (en) * 2006-02-23 2011-11-08 Nikon Corporation Spectral image processing method, computer-executable spectral image processing program, and spectral imaging system
US7643972B2 (en) * 2006-12-07 2010-01-05 Sas Institute Inc. Computer-implemented systems and methods for determining steady-state confidence intervals
US20110125466A1 (en) * 2009-11-20 2011-05-26 Lada Emily K Computer-Implemented Systems And Methods For Determining Steady-State Confidence Intervals
JP2013525819A (en) * 2010-05-06 2013-06-20 アバクシス， インコーポレイテッド Verification method of point-of-care test results by evaluating expected sample relationships
EP2588859B1 (en) 2010-06-29 2019-05-22 Canon U.S. Life Sciences, Inc. System and method for genotype analysis
JP5834584B2 (en) * 2011-07-25 2015-12-24 ソニー株式会社 Information processing apparatus, information processing method, program, and fluorescence spectrum intensity correction method
WO2013109592A1 (en) * 2012-01-16 2013-07-25 Leco Corporation Systems and methods to process and group chromatographic peaks
JP5870851B2 (en) * 2012-05-29 2016-03-01 ソニー株式会社 Information processing apparatus, information processing method, and program
CN103018441A (en) * 2012-12-31 2013-04-03 西南大学 Multicomponent immunoassay method based on time-resolved chemiluminescence
DE102014200446B3 (en) * 2014-01-13 2015-01-08 Technische Universität Dresden Process for the deconvolution of nucleic acid-containing substance mixtures
CA3097583A1 (en) * 2018-04-19 2019-10-24 Omniome, Inc. Improving accuracy of base calls in nucleic acid sequencing methods
CN111310618A (en) * 2020-02-03 2020-06-19 北京百度网讯科技有限公司 Article identification method and device, electronic equipment and readable storage medium

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4962037A (en) * 1987-10-07 1990-10-09 United States Of America Method for rapid base sequencing in DNA and RNA
US5614386A (en) * 1995-06-23 1997-03-25 Baylor College Of Medicine Alternative dye-labeled primers for automated DNA sequencing

Also Published As

Publication number Publication date
US6015667A (en) 2000-01-18
DE69705834T2 (en) 2001-12-06
EP0897568A1 (en) 1999-02-24
AU707113B2 (en) 1999-07-01
JP3287361B2 (en) 2002-06-04
WO1997046963A1 (en) 1997-12-11
DE69705834D1 (en) 2001-08-30
EP0897568B1 (en) 2001-07-25
AU3007497A (en) 1998-01-05
CA2256686C (en) 2003-01-21
JP2000503774A (en) 2000-03-28
ATE203605T1 (en) 2001-08-15

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Effective date: 20070515