CA2258512A1 - Amidases, nucleic acids encoding them and methods for making and using them - Google Patents
Amidases, nucleic acids encoding them and methods for making and using themInfo
- Publication number
- CA2258512A1 CA2258512A1 CA002258512A CA2258512A CA2258512A1 CA 2258512 A1 CA2258512 A1 CA 2258512A1 CA 002258512 A CA002258512 A CA 002258512A CA 2258512 A CA2258512 A CA 2258512A CA 2258512 A1 CA2258512 A1 CA 2258512A1
- Authority
- CA
- Canada
- Prior art keywords
- enzyme
- methods
- amidases
- making
- nucleic acids
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
Abstract
A purified thermostable enzyme is derived from the archael bacterium Thermococcus GU5L5. The enzyme has a molecular weight of about 68.5 kilodaltons and has cellulase activity. The enzyme can be produced from native or recombinant host cells and can be used for the removal of arginine, phenylalanine, or methionine amino acids from the N-terminal end of peptides in peptide or peptidomimetic synthesis. The enzyme is selective for the L, or "natural" enantiomer of the amino acid derivatives and is therefore useful for the production of optically active compounds. These reactions can be performed in the presence of the chemically more reactive ester functionality, a step which is very difficult to achieve with nonenzymatic methods.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/664,646 | 1996-06-17 | ||
US08/664,646 US5877001A (en) | 1996-06-17 | 1996-06-17 | Amidase |
PCT/US1997/009319 WO1997048794A1 (en) | 1996-06-17 | 1997-06-17 | Amidase |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2258512A1 true CA2258512A1 (en) | 1997-12-24 |
CA2258512C CA2258512C (en) | 2009-01-20 |
Family
ID=24666850
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002258512A Expired - Fee Related CA2258512C (en) | 1996-06-17 | 1997-06-17 | Amidases, nucleic acids encoding them and methods for making and using them |
Country Status (9)
Country | Link |
---|---|
US (8) | US5877001A (en) |
EP (1) | EP0937139B1 (en) |
JP (1) | JP4306802B2 (en) |
AT (1) | ATE275205T1 (en) |
AU (1) | AU723433B2 (en) |
CA (1) | CA2258512C (en) |
DE (1) | DE69730514T2 (en) |
ES (1) | ES2227701T3 (en) |
WO (1) | WO1997048794A1 (en) |
Families Citing this family (54)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
US6309883B1 (en) | 1994-02-17 | 2001-10-30 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
US20020137185A1 (en) * | 1996-06-17 | 2002-09-26 | Jay Short | Enzymes having amidase activity and methods of use thereof |
US5877001A (en) * | 1996-06-17 | 1999-03-02 | Diverso Corporation | Amidase |
KR100570935B1 (en) | 1997-01-17 | 2006-04-13 | 맥시겐, 인크. | Evolution of whole cells and organisms by recursive sequence recombination |
US6326204B1 (en) | 1997-01-17 | 2001-12-04 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
US7148054B2 (en) * | 1997-01-17 | 2006-12-12 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
US6723550B1 (en) * | 1997-07-15 | 2004-04-20 | Genencor International, Inc. | Proteases from gram-positive organisms |
US6153352A (en) * | 1997-12-10 | 2000-11-28 | Fuji Photo Film Co., Ltd. | Planographic printing plate precursor and a method for producing a planographic printing plate |
US6541011B2 (en) | 1998-02-11 | 2003-04-01 | Maxygen, Inc. | Antigen library immunization |
US7390619B1 (en) * | 1998-02-11 | 2008-06-24 | Maxygen, Inc. | Optimization of immunomodulatory properties of genetic vaccines |
WO1999057128A1 (en) | 1998-05-01 | 1999-11-11 | Maxygen, Inc. | Optimization of pest resistance genes using dna shuffling |
US7153655B2 (en) * | 1998-06-16 | 2006-12-26 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence |
US20060242731A1 (en) * | 1998-08-12 | 2006-10-26 | Venkiteswaran Subramanian | DNA shuffling to produce herbicide selective crops |
JP2002522072A (en) | 1998-08-12 | 2002-07-23 | マキシジェン, インコーポレイテッド | DNA shuffling of monooxygenase gene for production of industrial chemicals. |
AU6510799A (en) | 1998-10-07 | 2000-04-26 | Maxygen, Inc. | Dna shuffling to produce nucleic acids for mycotoxin detoxification |
WO2000028018A1 (en) | 1998-11-10 | 2000-05-18 | Maxygen, Inc. | Modified adp-glucose pyrophosphorylase for improvement and optimization of plant phenotypes |
US6438561B1 (en) * | 1998-11-19 | 2002-08-20 | Navigation Technologies Corp. | Method and system for using real-time traffic broadcasts with navigation systems |
US6917882B2 (en) | 1999-01-19 | 2005-07-12 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
US20030054390A1 (en) * | 1999-01-19 | 2003-03-20 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
EP1151409A1 (en) * | 1999-01-18 | 2001-11-07 | Maxygen, Inc. | Methods of populating data stuctures for use in evolutionary simulations |
US6436675B1 (en) | 1999-09-28 | 2002-08-20 | Maxygen, Inc. | Use of codon-varied oligonucleotide synthesis for synthetic shuffling |
US6376246B1 (en) | 1999-02-05 | 2002-04-23 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
US6961664B2 (en) | 1999-01-19 | 2005-11-01 | Maxygen | Methods of populating data structures for use in evolutionary simulations |
US20070065838A1 (en) * | 1999-01-19 | 2007-03-22 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
US7024312B1 (en) | 1999-01-19 | 2006-04-04 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
EP2253704B1 (en) | 1999-01-19 | 2015-08-19 | Codexis Mayflower Holdings, LLC | Oligonucleotide mediated nucleic acid recombination |
IL144657A0 (en) | 1999-02-11 | 2002-06-30 | Maxygen Inc | High throughput mass spectrometry |
JP3399518B2 (en) * | 1999-03-03 | 2003-04-21 | インターナショナル・ビジネス・マシーンズ・コーポレーション | Semiconductor structure and method of manufacturing the same |
EP1165775A2 (en) | 1999-03-05 | 2002-01-02 | Maxygen, Inc. | Recombination of insertion modified nucleic acids |
US6531316B1 (en) | 1999-03-05 | 2003-03-11 | Maxyag, Inc. | Encryption of traits using split gene sequences and engineered genetic elements |
WO2001004287A1 (en) * | 1999-07-07 | 2001-01-18 | Maxygen Aps | A method for preparing modified polypeptides |
US20040002474A1 (en) * | 1999-10-07 | 2004-01-01 | Maxygen Inc. | IFN-alpha homologues |
US7430477B2 (en) * | 1999-10-12 | 2008-09-30 | Maxygen, Inc. | Methods of populating data structures for use in evolutionary simulations |
US6686515B1 (en) | 1999-11-23 | 2004-02-03 | Maxygen, Inc. | Homologous recombination in plants |
US7115712B1 (en) * | 1999-12-02 | 2006-10-03 | Maxygen, Inc. | Cytokine polypeptides |
CN1301735A (en) * | 1999-12-24 | 2001-07-04 | 上海博德基因开发有限公司 | New polypeptide-amidase family protein 10 and polynucleotide coding such polypeptide |
SG121902A1 (en) * | 2000-01-11 | 2006-05-26 | Maxygen Inc | Integrated systems for diversity generation and screening |
AU2001241939A1 (en) * | 2000-02-28 | 2001-09-12 | Maxygen, Inc. | Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation |
AU2001287273A1 (en) * | 2000-03-24 | 2001-10-08 | Maxygen, Inc. | Methods for modulating cellular and organismal phenotypes |
WO2002000897A2 (en) | 2000-06-23 | 2002-01-03 | Maxygen, Inc. | Novel chimeric promoters |
AU2001272978A1 (en) | 2000-06-23 | 2002-01-08 | Maxygen, Inc. | Novel co-stimulatory molecules |
WO2002004629A2 (en) * | 2000-07-07 | 2002-01-17 | Maxygen, Inc. | Molecular breeding of transposable elements |
US6858422B2 (en) | 2000-07-13 | 2005-02-22 | Codexis, Inc. | Lipase genes |
EP1354031A2 (en) * | 2000-07-31 | 2003-10-22 | Maxygen, Inc. | Nucleotide incorporating enzymes |
WO2002016583A2 (en) * | 2000-08-24 | 2002-02-28 | Maxygen, Inc. | Constructs and their use in metabolic pathway engineering |
US6958213B2 (en) * | 2000-12-12 | 2005-10-25 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function |
US20020086292A1 (en) | 2000-12-22 | 2002-07-04 | Shigeaki Harayama | Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules |
WO2003020929A1 (en) * | 2001-08-28 | 2003-03-13 | Mitsubishi Rayon Co., Ltd. | Novel amide hydrolase gene |
JP4372679B2 (en) * | 2002-05-17 | 2009-11-25 | アリゲーター・バイオサイエンス・アーベー | In vitro molecular evolution of protein function |
WO2006069129A1 (en) * | 2004-12-22 | 2006-06-29 | E.I. Dupont De Nemours And Company | Method for the production of glycolic acid from ammonium glycolate by direct deammoniation |
GB2432366B (en) * | 2005-11-19 | 2007-11-21 | Alligator Bioscience Ab | A method for in vitro molecular evolution of protein function |
CN103382464B (en) * | 2013-07-22 | 2015-12-23 | 上海交通大学 | Derive from the Ntn hydrolase of the ancient bacterium of hyperthermophilic and encoding gene thereof and application |
JP7254299B2 (en) * | 2017-08-30 | 2023-04-10 | 国立大学法人 東京大学 | Drug-supporting thin film |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4014564C1 (en) * | 1990-05-07 | 1991-07-18 | Forschungszentrum Juelich Gmbh, 5170 Juelich, De | |
CA2119188A1 (en) * | 1993-04-05 | 1994-10-06 | Patricia A. Spears | Detection and identification of mycobacteria |
US5451522A (en) * | 1994-07-15 | 1995-09-19 | Eli Lilly And Company | Genes encoding and method of expressing an enzyme: phthalyl amidase |
US5877001A (en) * | 1996-06-17 | 1999-03-02 | Diverso Corporation | Amidase |
-
1996
- 1996-06-17 US US08/664,646 patent/US5877001A/en not_active Expired - Lifetime
-
1997
- 1997-06-17 JP JP50301198A patent/JP4306802B2/en not_active Expired - Fee Related
- 1997-06-17 EP EP97929740A patent/EP0937139B1/en not_active Expired - Lifetime
- 1997-06-17 DE DE69730514T patent/DE69730514T2/en not_active Expired - Lifetime
- 1997-06-17 CA CA002258512A patent/CA2258512C/en not_active Expired - Fee Related
- 1997-06-17 ES ES97929740T patent/ES2227701T3/en not_active Expired - Lifetime
- 1997-06-17 AU AU33728/97A patent/AU723433B2/en not_active Ceased
- 1997-06-17 AT AT97929740T patent/ATE275205T1/en not_active IP Right Cessation
- 1997-06-17 WO PCT/US1997/009319 patent/WO1997048794A1/en active IP Right Grant
- 1997-10-15 US US08/951,088 patent/US6136583A/en not_active Expired - Lifetime
-
1998
- 1998-04-24 US US09/066,285 patent/US5985646A/en not_active Expired - Lifetime
-
1999
- 1999-03-02 US US09/261,006 patent/US6004796A/en not_active Expired - Lifetime
- 1999-10-25 US US09/427,372 patent/US6500659B1/en not_active Expired - Lifetime
-
2000
- 2000-06-30 US US09/609,570 patent/US6465204B1/en not_active Expired - Lifetime
- 2000-06-30 US US09/609,566 patent/US6429004B1/en not_active Expired - Fee Related
- 2000-10-20 US US09/693,554 patent/US6525190B1/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
US5877001A (en) | 1999-03-02 |
WO1997048794A1 (en) | 1997-12-24 |
AU3372897A (en) | 1998-01-07 |
EP0937139B1 (en) | 2004-09-01 |
US6004796A (en) | 1999-12-21 |
JP4306802B2 (en) | 2009-08-05 |
US6429004B1 (en) | 2002-08-06 |
ATE275205T1 (en) | 2004-09-15 |
US6136583A (en) | 2000-10-24 |
EP0937139A4 (en) | 2000-08-02 |
AU723433B2 (en) | 2000-08-24 |
US6525190B1 (en) | 2003-02-25 |
US5985646A (en) | 1999-11-16 |
ES2227701T3 (en) | 2005-04-01 |
CA2258512C (en) | 2009-01-20 |
US6500659B1 (en) | 2002-12-31 |
JP2000512849A (en) | 2000-10-03 |
US6465204B1 (en) | 2002-10-15 |
DE69730514T2 (en) | 2005-09-01 |
EP0937139A1 (en) | 1999-08-25 |
DE69730514D1 (en) | 2004-10-07 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |
Effective date: 20130618 |