CA2260973A1 - Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon - Google Patents

Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon

Info

Publication number
CA2260973A1
CA2260973A1 CA002260973A CA2260973A CA2260973A1 CA 2260973 A1 CA2260973 A1 CA 2260973A1 CA 002260973 A CA002260973 A CA 002260973A CA 2260973 A CA2260973 A CA 2260973A CA 2260973 A1 CA2260973 A1 CA 2260973A1
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CA
Canada
Prior art keywords
oligonucleotides
acceptor
nucleic acid
primers
donor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002260973A
Other languages
French (fr)
Other versions
CA2260973C (en
Inventor
Irina A. Nazarenko
Satish K. Bhatnagar
Emily S. Winn-Deen
Robert J. Hohman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EMD Millipore Corp
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/778,487 external-priority patent/US5866336A/en
Application filed by Individual filed Critical Individual
Publication of CA2260973A1 publication Critical patent/CA2260973A1/en
Application granted granted Critical
Publication of CA2260973C publication Critical patent/CA2260973C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Abstract

The present invention provides labeled nucleic acid amplification oligonucleotides, which can be linear or hairpin primers or blocking oligonucleotides. The oligonucleotides of the invention are labeled with donor and/or acceptor moieties of molecular energy transfer pairs. The moieties can be fluorophores, such that fluorescent energy emitted by the donor is absorbed by the acceptor. The acceptor may be a fluorophore that fluoresces at a wavelength different from the donor moiety, or it may be a quencher. The oligonucleotides of the invention are configured so that a donor moiety and an acceptor moiety are incorporated into the amplification product. The invention also provides methods and kits for directly detecting amplification products employing the nucleic acid amplification primers. When labeled linear primers are used, treatment with exonuclease or by using specific temperature eliminates the need for separation of unincorporated primers. This "closed-tube" format greatly reduces the possibility of carryover contamination with amplification products, provides for high throughput of samples, and may be totally automated.
CA002260973A 1996-07-16 1997-07-15 Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon Expired - Lifetime CA2260973C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US68366796A 1996-07-16 1996-07-16
US08/683,667 1996-07-16
US08/778,487 US5866336A (en) 1996-07-16 1997-01-03 Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US08/778,487 1997-01-03
US08/837,034 US6117635A (en) 1996-07-16 1997-04-11 Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US08/837,034 1997-04-11
PCT/US1997/012315 WO1998002449A1 (en) 1996-07-16 1997-07-15 Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon

Publications (2)

Publication Number Publication Date
CA2260973A1 true CA2260973A1 (en) 1998-01-22
CA2260973C CA2260973C (en) 2003-03-18

Family

ID=27418429

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002260973A Expired - Lifetime CA2260973C (en) 1996-07-16 1997-07-15 Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon

Country Status (9)

Country Link
US (2) US6117635A (en)
EP (1) EP0912597B1 (en)
JP (2) JP3597869B2 (en)
AT (1) ATE305476T1 (en)
AU (1) AU3728597A (en)
CA (1) CA2260973C (en)
DE (1) DE69734283T2 (en)
ES (1) ES2248852T3 (en)
WO (1) WO1998002449A1 (en)

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