CA2260973A1 - Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon - Google Patents

Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon

Info

Publication number
CA2260973A1
CA2260973A1 CA002260973A CA2260973A CA2260973A1 CA 2260973 A1 CA2260973 A1 CA 2260973A1 CA 002260973 A CA002260973 A CA 002260973A CA 2260973 A CA2260973 A CA 2260973A CA 2260973 A1 CA2260973 A1 CA 2260973A1
Authority
CA
Canada
Prior art keywords
oligonucleotides
acceptor
nucleic acid
primers
donor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002260973A
Other languages
French (fr)
Other versions
CA2260973C (en
Inventor
Irina A. Nazarenko
Satish K. Bhatnagar
Emily S. Winn-Deen
Robert J. Hohman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EMD Millipore Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/778,487 external-priority patent/US5866336A/en
Application filed by Individual filed Critical Individual
Publication of CA2260973A1 publication Critical patent/CA2260973A1/en
Application granted granted Critical
Publication of CA2260973C publication Critical patent/CA2260973C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Abstract

The present invention provides labeled nucleic acid amplification oligonucleotides, which can be linear or hairpin primers or blocking oligonucleotides. The oligonucleotides of the invention are labeled with donor and/or acceptor moieties of molecular energy transfer pairs. The moieties can be fluorophores, such that fluorescent energy emitted by the donor is absorbed by the acceptor. The acceptor may be a fluorophore that fluoresces at a wavelength different from the donor moiety, or it may be a quencher. The oligonucleotides of the invention are configured so that a donor moiety and an acceptor moiety are incorporated into the amplification product. The invention also provides methods and kits for directly detecting amplification products employing the nucleic acid amplification primers. When labeled linear primers are used, treatment with exonuclease or by using specific temperature eliminates the need for separation of unincorporated primers. This "closed-tube" format greatly reduces the possibility of carryover contamination with amplification products, provides for high throughput of samples, and may be totally automated.
CA002260973A 1996-07-16 1997-07-15 Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon Expired - Lifetime CA2260973C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US68366796A 1996-07-16 1996-07-16
US08/683,667 1996-07-16
US08/778,487 US5866336A (en) 1996-07-16 1997-01-03 Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US08/778,487 1997-01-03
US08/837,034 1997-04-11
US08/837,034 US6117635A (en) 1996-07-16 1997-04-11 Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
PCT/US1997/012315 WO1998002449A1 (en) 1996-07-16 1997-07-15 Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon

Publications (2)

Publication Number Publication Date
CA2260973A1 true CA2260973A1 (en) 1998-01-22
CA2260973C CA2260973C (en) 2003-03-18

Family

ID=27418429

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002260973A Expired - Lifetime CA2260973C (en) 1996-07-16 1997-07-15 Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon

Country Status (9)

Country Link
US (2) US6117635A (en)
EP (1) EP0912597B1 (en)
JP (2) JP3597869B2 (en)
AT (1) ATE305476T1 (en)
AU (1) AU3728597A (en)
CA (1) CA2260973C (en)
DE (1) DE69734283T2 (en)
ES (1) ES2248852T3 (en)
WO (1) WO1998002449A1 (en)

Families Citing this family (311)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070269799A9 (en) * 1994-06-22 2007-11-22 Zhang David Y Nucleic acid amplification methods
US5854033A (en) * 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
ATE199572T1 (en) * 1995-11-21 2001-03-15 Univ Yale UNIMOLECULAR SEGMENT AMPLIFICATION AND DETERMINATION
US7070925B1 (en) 1996-07-16 2006-07-04 Gen-Probe Incorporated Method for determining the presence of an RNA analyte in a sample using a modified oligonucleotide probe
DK0912767T3 (en) * 1996-07-16 2006-10-30 Gen Probe Inc Method for Tracking and Amplifying Nucleic Acid Sequences Using Modified Oligonucleotides with Enhanced Target Specific TM
US5928869A (en) * 1997-05-30 1999-07-27 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
GB9725237D0 (en) * 1997-11-29 1998-01-28 Secr Defence Amplification system
WO1999029905A2 (en) * 1997-12-12 1999-06-17 Intergen Company Method for detecting apoptosis using fret labeled oligonucleotides
US6117986A (en) * 1998-06-10 2000-09-12 Intergen Company, L.P. Pyrimidines linked to a quencher
CA2335026A1 (en) * 1998-06-12 1999-12-16 Intergen Company Multi-fluorescent hairpin energy transfer oligonucleotides
GB9812768D0 (en) * 1998-06-13 1998-08-12 Zeneca Ltd Methods
WO2000001850A2 (en) 1998-07-02 2000-01-13 Gen-Probe Incorporated Molecular torches
US20100130368A1 (en) * 1998-07-30 2010-05-27 Shankar Balasubramanian Method and system for sequencing polynucleotides
US20030022207A1 (en) * 1998-10-16 2003-01-30 Solexa, Ltd. Arrayed polynucleotides and their use in genome analysis
DE19858588B4 (en) * 1998-08-22 2016-04-07 Qiagen Gmbh Dye-labeled oligonucleotide for labeling a nucleic acid molecule
WO2000044940A2 (en) 1999-01-28 2000-08-03 Gen-Probe Incorporated Nucleic acid sequences for detecting genetic markers for cancer in a biological sample
AUPP844899A0 (en) * 1999-02-01 1999-02-25 University Of Western Australia, The A method for detecting methylated nucleic acids
US6521428B1 (en) * 1999-04-21 2003-02-18 Genome Technologies, Llc Shot-gun sequencing and amplification without cloning
US6331393B1 (en) 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
US6277607B1 (en) 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
FR2794755A1 (en) * 1999-06-14 2000-12-15 Inst Nat Sante Rech Med PROCESS FOR CIRCULARIZING OLIGONUCLEOTIDES AROUND A DOUBLE-STRANDED NUCLEIC ACID, THE STRUCTURES OBTAINED AND THEIR APPLICATIONS
US20020025519A1 (en) 1999-06-17 2002-02-28 David J. Wright Methods and oligonucleotides for detecting nucleic acid sequence variations
WO2000079009A2 (en) * 1999-06-22 2000-12-28 Invitrogen Corporation Improved primers and methods for the detection and discrimination of nucleic acids
US6830902B1 (en) * 1999-07-02 2004-12-14 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
GB9917307D0 (en) * 1999-07-23 1999-09-22 Sec Dep Of The Home Department Improvements in and relating to analysis of DNA
PT1870417E (en) * 1999-07-27 2012-06-01 Bristol Myers Squibb Co Peptide acceptor ligation methods
GB9918237D0 (en) * 1999-08-04 1999-10-06 Secr Defence Detection system
WO2001036668A1 (en) 1999-11-16 2001-05-25 Atto-Tec Gmbh Colour labelled oligonucleotide for labelling a nucleic acid molecule
CA2360342A1 (en) 1999-12-02 2001-06-07 Molecular Staging Inc. Generation of single-strand circular dna from linear self-annealing segments
DE19959691A1 (en) * 1999-12-06 2001-08-16 Epigenomics Ag Method for the parallel detection of the methylation state of genomic DNA
GB2359625B (en) * 1999-12-10 2004-10-20 Molecular Light Tech Res Ltd Monitoring oligonucleotide binding process using chemiluminescence quenching
US7205129B1 (en) 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
EP1313881A2 (en) 2000-06-06 2003-05-28 TM Bioscience Corporation Capture moieties for nucleic acids and uses thereof
DE10029915B4 (en) * 2000-06-19 2005-06-16 Epigenomics Ag Method for the detection of cytosine methylations
US6531283B1 (en) 2000-06-20 2003-03-11 Molecular Staging, Inc. Protein expression profiling
US6686157B2 (en) 2000-06-30 2004-02-03 Molecular Staging Inc. Signal amplification with lollipop probes
AU2001283056A1 (en) * 2000-07-27 2002-02-13 California Institute Of Technology A rapid, quantitative method for the mass spectrometric analysis of nucleic acids for gene expression and genotyping
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
ATE347619T1 (en) * 2000-08-25 2006-12-15 Lovelace Respiratory Res Inst METHOD FOR CANCER DETECTION USING NESTED (ßNESTEDß) METHYLATION-SPECIFIC PCR
US6582920B2 (en) * 2000-09-01 2003-06-24 Gen-Probe Incorporated Amplification of HIV-1 RT sequences for detection of sequences associated with drug-resistance mutations
US20030087286A1 (en) * 2000-09-06 2003-05-08 Hodge Timothy A. Isolation of eukaryotic genomic DNA using magnetically responsive solid functionalized particles
EP1978110B1 (en) 2000-09-06 2010-05-26 Transnetyx, Inc. Computer-based method and system for screening genomic DNA
US20030082605A1 (en) * 2000-09-06 2003-05-01 Hodge Timothy A. Genomic DNA detection method and system thereof
US7494817B2 (en) * 2000-09-06 2009-02-24 Transnet Yx, Inc. Methods for genotype screening using magnetic particles
US20050272085A1 (en) * 2000-09-06 2005-12-08 Hodge Timothy A Methods for forensic and congenic screening
US20050266494A1 (en) * 2000-09-06 2005-12-01 Hodge Timothy A System and method for computer network ordering of biological testing
US20050239125A1 (en) * 2000-09-06 2005-10-27 Hodge Timothy A Methods for genotype screening
US20040166499A1 (en) 2000-10-05 2004-08-26 Yoshihide Hayashizaki Oligonucleotide linkers comprising a variable cohesive portion and method for the praparation of polynucleotide libraries by using said linkers
WO2002034947A2 (en) * 2000-10-24 2002-05-02 Intergen Company Detecting specific nucleotide sequences
WO2002061133A2 (en) 2000-11-09 2002-08-08 Yale University Nucleic acid detection using structured probes
US20020102557A1 (en) * 2001-01-31 2002-08-01 Gentile-Davey Maria C. Self -complementary molecular probe
ES2322559T3 (en) * 2001-02-27 2009-06-23 Virco Bvba CIRCULAR PROBE (CPA) AMPLIFICATION OF CIRCULARIZED NUCLEIC ACID MOLECULES.
US6573051B2 (en) * 2001-03-09 2003-06-03 Molecular Staging, Inc. Open circle probes with intramolecular stem structures
CN1279182C (en) * 2001-04-17 2006-10-11 纽约血液中心有限公司 Universal multi-variant detection system
CA2344599C (en) * 2001-05-07 2011-07-12 Bioneer Corporation Selective polymerase chain reaction of dna of which base sequence is completely unknown
GB0112868D0 (en) 2001-05-25 2001-07-18 Secr Defence Detection system
CA2348042A1 (en) 2001-06-04 2002-12-04 Ann Huletsky Sequences for detection and identification of methicillin-resistant staphylococcus aureus
US6977163B1 (en) 2001-06-13 2005-12-20 Caliper Life Sciences, Inc. Methods and systems for performing multiple reactions by interfacial mixing
US20020197611A1 (en) * 2001-06-21 2002-12-26 Chagovetz Alexander Michael Method for real-time detection and quantification of nucleic acid sequences using fluorescent primers
US7081336B2 (en) * 2001-06-25 2006-07-25 Georgia Tech Research Corporation Dual resonance energy transfer nucleic acid probes
US7297494B2 (en) * 2001-06-25 2007-11-20 Georgia Tech Research Corporation Activatable probes and methods for in vivo gene detection
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
WO2003089894A2 (en) * 2001-08-14 2003-10-30 Discovery, Inc. Isothermal amplification in nucleic acid analysis
US20060014186A1 (en) * 2001-09-04 2006-01-19 Hodge Timothy A Methods for genotype screening of a strain disposed on an adsorbent carrier
EP1436424A4 (en) * 2001-09-18 2005-11-16 Us Genomics Inc Differential tagging of polymers for high resolution linear analysis
US20030059850A1 (en) * 2001-09-26 2003-03-27 Psychiatric Genomics, Inc. Fluorescence proximity assay
US7297485B2 (en) * 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US6617137B2 (en) * 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
US6977148B2 (en) * 2001-10-15 2005-12-20 Qiagen Gmbh Multiple displacement amplification
US20030165859A1 (en) * 2001-10-23 2003-09-04 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
US6942972B2 (en) 2001-10-24 2005-09-13 Beckman Coulter, Inc. Efficient synthesis of protein-oligonucleotide conjugates
DE10154318A1 (en) * 2001-10-26 2003-05-15 Epigenomics Ag Method for analyzing genomic methylation patterns
CN1483076A (en) * 2001-10-26 2004-03-17 松下电器产业株式会社 Method of detecting target nucleic acid and nucleic acid probe
US20030148284A1 (en) * 2001-12-17 2003-08-07 Vision Todd J. Solid phase detection of nucleic acid molecules
US8323975B2 (en) * 2002-01-04 2012-12-04 The Board Of Trustees Of The Leland Stanford Junior University Telomere-encoding synthetic DNA nanocircles, and their use for the elongation of telomere repeats
EP1470147A4 (en) * 2002-01-04 2005-11-23 Univ Stanford Telomere-encoding synthetic dna nanocircles, and their use for the elongation of telomere repeats
JP2003299489A (en) * 2002-02-08 2003-10-21 Mitsubishi Chemicals Corp Nucleic acid construct
US20030180776A1 (en) * 2002-02-21 2003-09-25 Ming Wu Detection by sliding template amplification
US6818420B2 (en) 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same
EP1485056B1 (en) * 2002-02-27 2011-04-06 Life Technologies Corporation Methods of using fet labeled oligonucleotides that include a'5' exonuclease resistant quencher domain and compositions for practicing the same
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
WO2004000995A2 (en) * 2002-05-22 2003-12-31 Marshall University Methods, probes, and accessory molecules for detecting single nucleotide polymorphisms
US7282330B2 (en) * 2002-05-28 2007-10-16 U.S. Genomics, Inc. Methods and apparati using single polymer analysis
AU2003238796A1 (en) * 2002-05-28 2003-12-12 U.S. Genomics, Inc. Methods and apparati using single polymer analysis
EP1509624A2 (en) * 2002-05-31 2005-03-02 Secretary, Department Of Atomic Energy Met/fret based method of target nucleic acid detection whereby the donor/acceptor moieties are on complementary strands
EP1534857B1 (en) * 2002-06-20 2010-08-04 The Regents of The University of California Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores
US10001475B2 (en) 2002-06-20 2018-06-19 The Regents Of The University Of California Light harvesting multichromophore compositions and methods of using the same
US9371559B2 (en) 2002-06-20 2016-06-21 The Regents Of The University Of California Compositions for detection and analysis of polynucleotides using light harvesting multichromophores
US7144950B2 (en) 2003-09-17 2006-12-05 The Regents Of The University Of California Conformationally flexible cationic conjugated polymers
KR20050055717A (en) * 2002-08-26 2005-06-13 더 리전츠 오브 더 유니버시티 오브 캘리포니아 Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores
EP1549772B1 (en) * 2002-08-26 2013-01-02 The Regents of The University of California Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores
EP1537240A2 (en) * 2002-09-06 2005-06-08 Johns Hopkins Singapore Pte Ltd Strand specific detection and quantification
US20040219565A1 (en) 2002-10-21 2004-11-04 Sakari Kauppinen Oligonucleotides useful for detecting and analyzing nucleic acids of interest
TW200413527A (en) * 2002-10-29 2004-08-01 Riken Amplification of nucleic acid
WO2004050916A1 (en) * 2002-12-02 2004-06-17 Solexa Limited Recovery of original template
AU2003290223A1 (en) * 2002-12-02 2004-06-23 Solexa Limited Determination of methylation of nucleic acid sequences
US20040115643A1 (en) * 2002-12-12 2004-06-17 Lizardi Paul M. Thermodynamic equilibrium extension of primers
US7955795B2 (en) * 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production
JP5479663B2 (en) 2002-12-20 2014-04-23 セレラ コーポレーション Genetic polymorphism associated with myocardial infarction, detection method and use thereof
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
AU2003299867A1 (en) * 2002-12-27 2004-07-29 University Of Medicine And Dentistry Of New Jersey Method for single nucleotide polymorphism detection
WO2004068112A2 (en) * 2003-01-28 2004-08-12 Gorilla Genomics, Inc. Hairpin primer amplification
EP1599609B1 (en) * 2003-02-13 2009-11-11 The Regents of The University of California Methods and compositions for detection and analysis of polynucleotide-binding protein interactions using light harvesting multichromophores
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
JP4782668B2 (en) 2003-03-31 2011-09-28 エフ．ホフマン−ラ ロシュ アーゲー Compositions and methods for detecting certain flaviviruses, including members of the Japanese encephalitis virus serogroup
JP4336877B2 (en) * 2003-04-18 2009-09-30 アークレイ株式会社 Method for detecting β3 adrenergic receptor mutant gene and nucleic acid probe and kit therefor
CN1537954A (en) * 2003-04-18 2004-10-20 ������������ʽ���� Expressing genetic analysis method and probe reagent box for expressing genetic analysng
US8679789B2 (en) * 2003-05-01 2014-03-25 Gen-Probe Incorporated Oligonucleotides comprising a molecular switch
DE602004007382T2 (en) 2003-05-20 2008-04-17 Bayer Pharmaceuticals Corp., West Haven DIARYL UREAS FOR PDGFR MEDIATED DISEASES
US20040248103A1 (en) * 2003-06-04 2004-12-09 Feaver William John Proximity-mediated rolling circle amplification
EP1502958A1 (en) * 2003-08-01 2005-02-02 Roche Diagnostics GmbH New detection format for hot start real time polymerase chain reaction
DE10337668A1 (en) * 2003-08-08 2005-03-03 Wessig, Pablo, Dr. Molecular probe and material for detection of an analyte and their use
DE10338308B4 (en) * 2003-08-15 2006-10-19 Epigenomics Ag Method for the detection of cytosine methylations in DNA
US20050074779A1 (en) * 2003-10-02 2005-04-07 Tuan Vo-Dinh SERS molecular probe for diagnostics and therapy
US7901880B2 (en) 2003-10-21 2011-03-08 Orion Genomics Llc Differential enzymatic fragmentation
EP1689764B1 (en) * 2003-11-19 2013-01-02 AlleLogic Biosciences Corporation Oligonucleotides labeled with a plurality of fluorophores
JP5649263B2 (en) 2003-11-26 2015-01-07 セレラ コーポレーション Genetic polymorphisms associated with cardiovascular disorders and drug responses, methods for their detection and uses
EP2287341B1 (en) 2003-12-01 2013-02-13 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same
KR100542895B1 (en) * 2003-12-22 2006-01-11 재단법인 포항산업과학연구원 Method for controlling ballast water using effect of NaOCl produced electrolysis of natural seawater and an apparatus for the same
MX2009009225A (en) 2003-12-23 2009-09-28 Cythera Inc Definitive endoderm.
US20050266554A1 (en) * 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
US8647873B2 (en) 2004-04-27 2014-02-11 Viacyte, Inc. PDX1 expressing endoderm
US7541185B2 (en) * 2003-12-23 2009-06-02 Cythera, Inc. Methods for identifying factors for differentiating definitive endoderm
US7985585B2 (en) 2004-07-09 2011-07-26 Viacyte, Inc. Preprimitive streak and mesendoderm cells
US7625753B2 (en) * 2003-12-23 2009-12-01 Cythera, Inc. Expansion of definitive endoderm cells
US8586357B2 (en) 2003-12-23 2013-11-19 Viacyte, Inc. Markers of definitive endoderm
WO2005063977A1 (en) 2003-12-25 2005-07-14 Riken Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same
GB0406015D0 (en) * 2004-03-17 2004-04-21 Dynal Biotech Asa Improvements in magnetic polymer particles
WO2005093095A1 (en) * 2004-03-24 2005-10-06 Epigenomics Ag Method for analysis of cytosine methylation
US8192937B2 (en) * 2004-04-07 2012-06-05 Exiqon A/S Methods for quantification of microRNAs and small interfering RNAs
EP2423327B1 (en) 2004-05-07 2014-01-08 Celera Corporation Genetic polymorphism associated with liver fibrosis methods of detection and uses thereof
US7575863B2 (en) * 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
JP4476050B2 (en) * 2004-06-30 2010-06-09 株式会社ニデック Perimeter
AU2005280162B2 (en) 2004-08-27 2012-04-26 Gen-Probe Incorporated Single-primer nucleic acid amplification methods
ATE494392T1 (en) * 2004-09-21 2011-01-15 Life Technologies Corp TWO-COLOR REAL-TIME/ENDPOINT QUANTIFICATION OF MICRO-RNAS (MIRNAS)
NZ554701A (en) * 2004-10-18 2010-03-26 Univ Brandeis Reagents and methods for improving reproducibility and reducing mispriming in PCR amplification
ES2391744T3 (en) 2004-11-01 2012-11-29 George Mason University Compositions and procedures for diagnosing colon disorders
US20060105348A1 (en) * 2004-11-15 2006-05-18 Lee Jun E Compositions and methods for the detection and discrimination of nucleic acids
US20060275792A1 (en) * 2004-11-15 2006-12-07 Lee Jun E Enhancement of nucleic acid amplification using double-stranded DNA binding proteins
US20060188902A1 (en) * 2005-01-03 2006-08-24 The Gov. of the USA as represented by the Secretary of the Dept. of Health and Human Primer for nucleic acid detection
CA2594458C (en) 2005-01-10 2016-08-23 The Regents Of The University Of California Cationic conjugated polymers suitable for strand-specific polynucleotide detection in homogeneous and solid state assays
WO2006074482A2 (en) * 2005-01-10 2006-07-13 The Regents Of The University Of California Methods and kits for strand-specific polynucleotide detection with cationic multichromophores
US7666594B2 (en) * 2005-01-31 2010-02-23 The Regents Of The University Of California Methods for assaying a sample for an aggregant
GB0502010D0 (en) * 2005-02-01 2005-03-09 Enigma Diagnostics Ltd Biochemical reagents and their uses
US8192938B2 (en) * 2005-02-24 2012-06-05 The Ohio State University Methods for quantifying microRNA precursors
CA2600794C (en) 2005-03-11 2014-08-12 Applera Corporation Genetic polymorphisms associated with coronary heart disease, methods of detection and uses thereof
US8309303B2 (en) 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
US20070111226A1 (en) * 2005-08-24 2007-05-17 Applera Corporation Method to Quantify siRNAs, miRNAs and Polymorphic miRNAs
EP1762627A1 (en) 2005-09-09 2007-03-14 Qiagen GmbH Method for the activation of a nucleic acid for performing a polymerase reaction
US7799530B2 (en) 2005-09-23 2010-09-21 Celera Corporation Genetic polymorphisms associated with cardiovascular disorders and drug response, methods of detection and uses thereof
ATE519863T1 (en) * 2005-10-05 2011-08-15 Hoffmann La Roche NON-FLUORESCENT ENERGY TRANSFER
US11834720B2 (en) 2005-10-11 2023-12-05 Geneohm Sciences, Inc. Sequences for detection and identification of methicillin-resistant Staphylococcus aureus (MRSA) of MREJ types xi to xx
US7838221B2 (en) 2005-10-11 2010-11-23 Geneohm Sciences, Inc. Sequences for detection and identification of methicillin-resistant Staphylococcus aureus (MRSA)
EP1960542A4 (en) * 2005-12-05 2009-05-13 Temasek Life Sciences Lab Ltd Fluorescent primer system for detection of nucleic acids (q priming)
US20080057499A1 (en) * 2006-02-06 2008-03-06 Affymetrix, Inc. Methods for high specificity whole genome amplification and hybridization
GB0605584D0 (en) * 2006-03-20 2006-04-26 Olink Ab Method for analyte detection using proximity probes
AU2007233270B2 (en) * 2006-04-04 2013-06-20 Fluidigm Corporation Cooperative probes and methods of using them
FR2900158A1 (en) * 2006-04-25 2007-10-26 Biomerieux Sa Probe for detecting a target sequence comprises a first nucleotide segment with a sterically hindering structure at its 5' end and a second nucleotide segment complementary to the target sequence
US7989204B2 (en) * 2006-04-28 2011-08-02 Viacyte, Inc. Hepatocyte lineage cells
US20100216657A1 (en) * 2006-05-16 2010-08-26 Arcxis Biotechnologies, Inc. Pcr-free sample preparation and detection systems for high speed biologic analysis and identification
US7833716B2 (en) * 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
JP2008029333A (en) * 2006-07-03 2008-02-14 Fujifilm Corp Primer for use in new gene amplification method
EP2639318B1 (en) 2006-09-11 2015-04-08 Celera Corporation Genetic polymorphisms associated with Psoriasis, methods of detection and uses thereof
FR2906532B1 (en) * 2006-09-28 2008-12-12 Biomerieux Sa NEW OLIGONUCLEOTIDE BRAND
CA2915679C (en) 2006-10-20 2017-12-12 Celera Corporation Genetic polymorphisms associated with venous thrombosis, methods of detection and uses thereof
US9938641B2 (en) * 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
JP2010512799A (en) 2006-12-19 2010-04-30 ジーンオーム サイエンシーズ、インク． Detection of Staphylococcus aureus and identification of methicillin-resistant Staphylococcus aureus
ATE528286T1 (en) 2006-12-20 2011-10-15 Bayer Healthcare Llc 4-Ä4-Ä(Ä3-TERT-BUTYL-1-Ä3-(HYDROXYMETHYL)PHENYL - 1H-PYRAZOLE-5-YLÜCARBAMOYL)-AMINOÜ-3- CHLOROPHENOXYÜ-N-METHYLPYRIDINE-2-CARBOXAMIDE AS AN INHIBITOR OF THE VEGFR KINASE FOR TREATMENT OF CANCER
EP2520668B1 (en) 2007-01-31 2014-12-24 Celera Corporation A molecular prognostic signature for predicting breast cancer distant metastasis, and uses thereof
WO2008096146A1 (en) * 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
US8017104B2 (en) * 2007-02-28 2011-09-13 Carestream Health, Inc. Large stoke shift dye used for optical imaging
US8906354B2 (en) 2007-02-28 2014-12-09 Bruker Biospin Corporation Loaded latex optical molecular imaging probes containing lipophilic large stokes shift dyes
US8183359B2 (en) 2007-03-01 2012-05-22 Gen-Probe Incorporated Kits for amplifying DNA
AU2008260029B2 (en) 2007-05-31 2015-02-12 Yale University A genetic lesion associated with cancer
US9689031B2 (en) * 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
EP2952588A1 (en) 2007-08-06 2015-12-09 Orion Genomics, LLC Single nucleotide polymorphism in igf2 for determining the allele-specific expression of the igf2 gene
US9050280B2 (en) * 2007-09-17 2015-06-09 Mdxhealth Sa Methylation detection of MGMT
US7695963B2 (en) * 2007-09-24 2010-04-13 Cythera, Inc. Methods for increasing definitive endoderm production
US8043978B2 (en) 2007-10-11 2011-10-25 Riken Electronic device and method for producing electronic device
US8039212B2 (en) 2007-11-05 2011-10-18 Celera Corporation Genetic polymorphisms associated with liver fibrosis, methods of detection and uses thereof
US8119656B2 (en) 2007-12-07 2012-02-21 The Board Of Regents Of The University Of Texas System Inhibitors of the influenza virus non-structural 1 protein
US20090221620A1 (en) 2008-02-20 2009-09-03 Celera Corporation Gentic polymorphisms associated with stroke, methods of detection and uses thereof
WO2009155271A1 (en) * 2008-06-17 2009-12-23 Sigma-Aldrich Co. Real time polymerase chain reaction process using a universal detection system
CN101619335B (en) * 2008-07-01 2012-04-18 中国人民解放军军事医学科学院基础医学研究所 Method for preparing single chain DNA by using primer with stem-loop sample structure through PCR
FR2933410B1 (en) * 2008-07-04 2010-08-20 Biomerieux Sa NEW DETECTION PROBE
EP2733222A1 (en) 2008-07-09 2014-05-21 Celera Corporation Genetic polymorphisms associated with cardiovascular diseases, methods of detection and uses thereof
GB0909333D0 (en) 2009-06-01 2009-07-15 Fu Guoliang Multiplex amplification and detection
EP2373808B1 (en) 2008-12-09 2015-11-25 Oxitec Limited Enhanced taqman probe based amplification
CA2751287A1 (en) 2009-02-06 2010-09-10 Yale University A snp marker of breast and ovarian cancer risk
EP2396429B1 (en) 2009-02-11 2015-05-27 Orion Genomics, LLC Combinations of polymorphisms for determining allele-specific expression of igf2
GB2480792B (en) * 2009-03-02 2018-11-14 Co Diagnostics Inc Energy transfer hairpin oligonucleotide probes
US9599591B2 (en) 2009-03-06 2017-03-21 California Institute Of Technology Low cost, portable sensor for molecular assays
US8993236B2 (en) * 2009-04-17 2015-03-31 California Institute Of Technology Electromagnetic molecular sensors and methods of using same
CN102575284A (en) 2009-03-17 2012-07-11 MDx健康公司 Improved detection of gene expression
WO2010147848A2 (en) 2009-06-15 2010-12-23 Rd Biosciences, Inc. Kits and methods for selective amplification and detection of nucleic acid targets
EP2446056A2 (en) 2009-06-25 2012-05-02 Yale University Single nucleotide polymorphisms in brca1 and cancer risk
JP5785942B2 (en) * 2009-06-29 2015-09-30 ルミネックス コーポレーション Chimeric primer with hairpin conformation and use thereof
KR20110050327A (en) 2009-11-07 2011-05-13 주식회사 씨젠 T h d primer target detection
EP4276190A3 (en) 2009-12-03 2023-12-27 Quest Diagnostics Investments Incorporated Methods for the diagnosis of bacterial vaginosis
RU2551321C2 (en) * 2009-12-21 2015-05-20 Сиджен, Инк. Target detection with tsg-primer
CN102762745A (en) * 2009-12-24 2012-10-31 Seegene株式会社 Real-time multiplexing detection of target nucleic acid sequences with elimination of false signals
US20110177512A1 (en) * 2010-01-19 2011-07-21 Predictive Biosciences, Inc. Method for assuring amplification of an abnormal nucleic acid in a sample
US8574832B2 (en) * 2010-02-03 2013-11-05 Massachusetts Institute Of Technology Methods for preparing sequencing libraries
EP2545384B1 (en) * 2010-03-12 2017-12-13 JPT Peptide Technologies GmbH Method for determining the concentration of a peptide
GB201004292D0 (en) * 2010-03-15 2010-04-28 Olink Ab Assay for localised detection of analytes
CN103079567A (en) 2010-04-17 2013-05-01 拜尔健康护理有限责任公司 Synthetic metabolites of fluoro substituted omega-carboxyaryl diphenyl urea for the treatment and prevention diseases and conditions
US20110269735A1 (en) 2010-04-19 2011-11-03 Celera Corporation Genetic polymorphisms associated with statin response and cardiovascular diseases, methods of detection and uses thereof
WO2011154139A2 (en) 2010-06-07 2011-12-15 Roche Diagnostics Gmbh Gene expression markers for predicting response to interleukin-6 receptor-inhibiting monoclonal antibody drug treatment
EP2580355B1 (en) 2010-06-14 2018-03-07 National University of Singapore Modified stem-loop oligonucleotide mediated reverse transcription and base-spacing constrained quantitative pcr
GB201011971D0 (en) 2010-07-15 2010-09-01 Olink Ab Methods and product
US8778614B2 (en) 2010-08-24 2014-07-15 Enzo Life Sciences, Inc. Assays for detecting modified compounds
US8557532B2 (en) 2010-08-30 2013-10-15 The University Of Utah Research Foundation Diagnosis and treatment of drug-resistant Ewing'S sarcoma
WO2012034130A2 (en) 2010-09-10 2012-03-15 Qiagen Methods and compositions for nucleic acid detection
US20120070829A1 (en) 2010-09-10 2012-03-22 Bio-Rad Laboratories, Inc. Size selection of dna for chromatin analysis
US10669569B2 (en) 2010-10-15 2020-06-02 Navinci Diagnostics Ab Dynamic range methods
GB201017978D0 (en) 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
US20120108651A1 (en) 2010-11-02 2012-05-03 Leiden University Medical Center (LUMC) Acting on Behalf of Academic Hospital Leiden (AZL) Genetic polymorphisms associated with venous thrombosis and statin response, methods of detection and uses thereof
WO2012095748A2 (en) * 2011-01-14 2012-07-19 Smiths Detection - Watford Limited Single tube quantitative polymerase chain reaction (pcr)
GB201101621D0 (en) 2011-01-31 2011-03-16 Olink Ab Method and product
EP2675913B1 (en) 2011-02-15 2016-12-14 Bio-Rad Laboratories, Inc. Detecting methylation in a subpopulation of genomic dna
US20140024590A1 (en) 2011-02-18 2014-01-23 Yale University KRAS-Variant And Endometriosis
US20140065615A1 (en) 2011-03-21 2014-03-06 Yale University The KRAS Variant and Tumor Biology
GB201107863D0 (en) 2011-05-11 2011-06-22 Olink Ab Method and product
US9109226B2 (en) * 2011-09-01 2015-08-18 New England Biolabs, Inc. Synthetic nucleic acids for polymerization reactions
US9586987B2 (en) 2011-09-08 2017-03-07 Kabushiki Kaisha Dnaform Primer set for isothermal amplication of a target nucleic acid sequence
CA2848240C (en) * 2011-09-16 2020-09-29 Lexogen Gmbh Nucleic acid transcription method
EP2570487A1 (en) * 2011-09-16 2013-03-20 Lexogen GmbH Nucleic acid transcription method
WO2013041577A1 (en) 2011-09-20 2013-03-28 Vib Vzw Methods for the diagnosis of amyotrophic lateral sclerosis and frontotemporal lobar degeneration
US9352312B2 (en) 2011-09-23 2016-05-31 Alere Switzerland Gmbh System and apparatus for reactions
US9416153B2 (en) 2011-10-11 2016-08-16 Enzo Life Sciences, Inc. Fluorescent dyes
US9382580B2 (en) * 2011-10-18 2016-07-05 Scanogen Inc. Detection units and methods for detecting a target analyte
GB201201547D0 (en) 2012-01-30 2012-03-14 Olink Ab Method and product
AU2013243300B2 (en) 2012-04-05 2018-12-06 Oregon Health & Science University Gene expression panel for breast cancer prognosis
CN104364395B (en) 2012-04-06 2018-03-23 基因欧姆科技加拿大公司 For detecting the sequence with identification of M REJ types xxi methicillin-resistant staphylococcus aureus (MRSA)
CN102796817B (en) * 2012-07-06 2014-06-25 陕西北美基因股份有限公司 Method for rapidly detecting mutation of KRAS gene
CN102796816B (en) * 2012-07-06 2014-04-16 陕西北美基因股份有限公司 Method for performing in-vitro accurate test on KRAS gene mutation
EP2900836B1 (en) * 2012-09-28 2023-05-10 Cepheid Two-primer pcr for microrna multiplex assay
US10174366B2 (en) 2012-11-14 2019-01-08 Olink Bioscience Ab Localised RCA-based amplification method
WO2014076214A1 (en) 2012-11-14 2014-05-22 Olink Ab Rca reporter probes and their use in detecting nucleic acid molecules
US9411930B2 (en) 2013-02-01 2016-08-09 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
AU2014212152B2 (en) 2013-02-01 2020-02-06 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
CN105339505B (en) 2013-03-12 2021-08-10 生命技术公司 Universal reporter-based genotyping methods and materials
WO2014145291A1 (en) 2013-03-15 2014-09-18 Theranos, Inc. Nucleic acid amplification
EP2925895B1 (en) 2013-03-15 2018-11-28 Avellino Lab USA, Inc. Methods for improved isolation of genomic dna templates for allele detection
WO2014145296A2 (en) 2013-03-15 2014-09-18 Theranos, Inc. Nuclei acid amplification
US9994916B2 (en) 2013-03-15 2018-06-12 Becton, Dickinson And Company Detection of neisseria gonorrhoeaes
JP2016512041A (en) * 2013-03-15 2016-04-25 アボツト・モレキユラー・インコーポレイテツド Multiple allele detection
ES2908751T3 (en) 2013-03-15 2022-05-03 Labrador Diagnostics Llc Nucleic acid amplification
US10450595B2 (en) 2013-03-15 2019-10-22 Theranos Ip Company, Llc Nucleic acid amplification
CA2917206C (en) * 2013-08-09 2021-09-21 Genebio Systems, Inc. Dna amplification via scissor-like structures (dasl)
GB2517700A (en) * 2013-08-27 2015-03-04 Lgc Ltd Oligonucleotides comprising a secondary structure and uses thereof
CA2920896A1 (en) 2013-09-06 2015-03-12 Theranos, Inc. Systems and methods for detecting infectious diseases
GB201320145D0 (en) 2013-11-14 2014-01-01 Olink Ab Localised RCA-based amplification method using a padlock-probe
KR102293785B1 (en) 2013-11-15 2021-08-26 아벨리노 랩 유에스에이, 인크. Methods for multiplex detection of alleles associated with ophthalmic conditions
WO2015085230A1 (en) 2013-12-06 2015-06-11 Bio-Rad Laboratories, Inc. Fusion polymerases
CA2933387C (en) 2013-12-11 2023-05-02 The Regents Of The University Of California Methods for labeling dna fragments to reconstruct physical linkage and phase
GB201401885D0 (en) 2014-02-04 2014-03-19 Olink Ab Proximity assay with detection based on hybridisation chain reaction (HCR)
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
EP3172332B1 (en) 2014-07-22 2020-10-14 Bio-rad Laboratories, Inc. Buffers for use with polymerases
CA2956925C (en) 2014-08-01 2024-02-13 Dovetail Genomics, Llc Tagging nucleic acids for sequence assembly
US20160115473A1 (en) * 2014-08-14 2016-04-28 Abbott Molecular Inc. Multifunctional oligonucleotides
BR112017006215A2 (en) * 2014-09-30 2018-03-06 Koninklijke Philips Nv method for detecting spatial proximity of first and second epitopes, method for stratifying an individual, kit for performing the method, and use of the kit
GB201417497D0 (en) 2014-10-03 2014-11-19 Convergence Pharmaceuticals Novel use
GB201417500D0 (en) 2014-10-03 2014-11-19 Convergence Pharmaceuticals Novel use
GB201417499D0 (en) 2014-10-03 2014-11-19 Convergence Pharmaceuticals Novel use
CA2976902A1 (en) 2015-02-17 2016-08-25 Dovetail Genomics Llc Nucleic acid sequence assembly
GB2554572B (en) 2015-03-26 2021-06-23 Dovetail Genomics Llc Physical linkage preservation in DNA storage
DK3286323T3 (en) 2015-04-24 2023-03-13 Becton Dickinson Co Multiplex detection of bacterial vaginosis
AU2016341198B2 (en) 2015-10-19 2023-03-09 Dovetail Genomics, Llc Methods for genome assembly, haplotype phasing, and target independent nucleic acid detection
GB201518655D0 (en) 2015-10-21 2015-12-02 Olink Ab Method for generating proximity probes
AU2016356474A1 (en) 2015-11-20 2018-06-21 Seegene, Inc. Method for calibrating a data set of a target analyte
US11485998B2 (en) 2015-12-15 2022-11-01 Seegene, Inc. Signal extraction for a target nucleic acid sequence
WO2017123696A1 (en) 2016-01-12 2017-07-20 Interleukin Genetics, Inc. Methods for predicting response to treatment
CN105624296A (en) * 2016-01-28 2016-06-01 屈强 Universal fluorescent hairpin primers for detecting gene polymorphism on basis of ARMS (amplification refractory mutation system) fluorescent PCR (polymerase chain reaction) process
EP3411819A4 (en) 2016-02-05 2019-10-23 Seegene, Inc. Method for reducing noise level of data set for a target analyte
KR20180116377A (en) 2016-02-23 2018-10-24 더브테일 제노믹스 엘엘씨 Generation and Hexagonal Paging of a Paged Read Set for Genome Assemblies
JP2019515035A (en) 2016-04-27 2019-06-06 ミラ ディーエックス， インコーポレイテッド Immunity-based treatment of KRAS variant cancer patients
EP3954771A1 (en) 2016-05-13 2022-02-16 Dovetail Genomics, LLC Recovering long-range linkage information from preserved samples
US11767566B2 (en) 2016-06-03 2023-09-26 Max-Planck-Gesellschaft Methods for the detection of a latent tuberculosis infection
KR102264865B1 (en) 2016-06-30 2021-06-14 주식회사 씨젠 Fluorescence-detecting device for analysis of reaction
GB201621514D0 (en) 2016-12-16 2017-02-01 Q-Linea Ab Padlock probe detection method
US10337070B2 (en) 2017-01-12 2019-07-02 Cardioforecast Ltd. Methods and kits for treating cardiovascular disease
WO2018175399A1 (en) 2017-03-24 2018-09-27 Bio-Rad Laboratories, Inc. Universal hairpin primers
CN110520543A (en) 2017-03-29 2019-11-29 中美冠科生物技术（太仓）有限公司 Determine gastric cancer to the system and method for Cetuximab sensibility
EP3628056A4 (en) 2017-04-28 2021-03-03 Avellino Lab USA, Inc. Methods for detecting alleles associated with keratoconus
WO2019045532A2 (en) 2017-08-31 2019-03-07 Seegene, Inc. Evaluation of performance of components using a pair of dimer-forming primers
EP3688188A4 (en) 2017-09-29 2021-06-16 Seegene, Inc. Detection of target nucleic acid sequences by pto cleavage and extension-dependent extension assay
CN111263819A (en) 2017-10-06 2020-06-09 卡特阿纳公司 RNA templated ligation
MX2020006923A (en) 2018-01-05 2020-09-09 Seegene Inc METHOD FOR DETERMINING THE PRESENCE OR ABSENCE <i>OF M.
JP7170062B2 (en) 2018-04-20 2022-11-11 シージーン アイエヌシー Methods and apparatus for detection of multiple target nucleic acid sequences within a sample
KR102220701B1 (en) 2018-09-28 2021-03-03 (주)나노헬릭스 Multiplex identification method of pcr product using fluorescence-based multiple melting analysis
CN109797200B (en) * 2019-02-13 2022-07-19 中国科学院苏州生物医学工程技术研究所 Ratio type telomerase activity quantitative detection method
EP3980561A1 (en) 2019-06-06 2022-04-13 Sitokine Limited Compositions and methods for treating lung, colorectal and breast cancer
US20220290246A1 (en) 2019-08-07 2022-09-15 Arizona Board Of Regents On Behalf Of The University Of Arizona Single nucleotide polymorphisms and uses thereof
WO2021028469A1 (en) 2019-08-12 2021-02-18 Sitokine Limited Compositions and methods for treating cytokine release syndrome and neurotoxicity
EP4033555B1 (en) * 2019-09-17 2023-11-01 BOE Technology Group Co., Ltd. Quantum dot ligand, quantum dot material, and quantum dot light emitting device
GB201919029D0 (en) 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
GB201919032D0 (en) 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
WO2021183941A1 (en) * 2020-03-13 2021-09-16 Uh-Oh Labs Inc. Looped primer and loop-de-loop method for detecting target nucleic acid
WO2021205013A1 (en) 2020-04-09 2021-10-14 Sitokine Limited Compositions and methods for treating covid-19
WO2021231779A1 (en) 2020-05-14 2021-11-18 Becton, Dickinson And Company Primers for immune repertoire profiling
WO2022095922A1 (en) 2020-11-05 2022-05-12 Becton, Dickinson And Company Rapid identification and typing of vibrio parahaemolyticus
CN116507742A (en) 2020-11-05 2023-07-28 贝克顿迪金森公司 Multiplex detection and typing of Vibrio cholerae
WO2022095924A1 (en) 2020-11-05 2022-05-12 Becton, Dickinson And Company Multiplex detection of bacterial respiratory pathogens
WO2023039433A1 (en) 2021-09-08 2023-03-16 Becton, Dickinson And Company Non-sequencing pcr-based method for detection of antibody-conjugated oligonucleotides
WO2023043280A1 (en) 2021-09-17 2023-03-23 주식회사 씨젠 Detection of target nucleic acid sequence by using synthetic non-natural base-bearing tag oligonucleotide
WO2023067110A1 (en) * 2021-10-20 2023-04-27 Tataa Biocenter Ab Methods and compositions for detection of mutant nucleic acid sequences
WO2024003792A1 (en) 2022-06-29 2024-01-04 Crispr Therapeutics Ag Viral detection assays
WO2024028818A1 (en) 2022-08-04 2024-02-08 Crispr Therapeutics Ag Method for detecting mycoplasma contamination
WO2024044352A1 (en) 2022-08-26 2024-02-29 The General Hospital Corporation Methods and compositions for prognosis and treatment of dilated cardiomyopathy and heart failure
WO2024054823A1 (en) * 2022-09-07 2024-03-14 Becton, Dickinson And Company Hairpin internal control for isothermal nucleic acid amplification

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2998943A (en) * 1954-08-12 1961-09-05 Billy D Dobbins Antenna cap
US3996345A (en) * 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4199559A (en) * 1974-08-12 1980-04-22 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4174384A (en) * 1975-06-30 1979-11-13 Syva Company Fluorescence quenching with immunological pairs in immunoassays
IT1079045B (en) * 1977-05-16 1985-05-08 Syva Co DOUBLE RECEPTOR FLUORESCENT IMUUNOLOGICAL ANALYSIS
CA1190838A (en) * 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
US5288609A (en) * 1984-04-27 1994-02-22 Enzo Diagnostics, Inc. Capture sandwich hybridization method and composition
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4996143A (en) * 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
CA1273552A (en) * 1985-12-23 1990-09-04 Michael J. Heller Fluorescent stokes shift probes for polynucleotide hybridization assays
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5079352A (en) * 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
DE3803275A1 (en) * 1988-02-04 1989-08-17 Dornier Medizintechnik PIEZOELECTRIC SHOCK WAVE SOURCE
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5118801A (en) * 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
WO1990011372A1 (en) * 1989-03-21 1990-10-04 Collaborative Research, Inc. Multiplex dna diagnostic test
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
JP3080178B2 (en) * 1991-02-18 2000-08-21 東洋紡績株式会社 Method for amplifying nucleic acid sequence and reagent kit therefor
WO1992014845A1 (en) * 1991-02-26 1992-09-03 Worcester Foundation For Experimental Biology Diagnosing cystic fibrosis and other genetic diseases using fluorescence resonance energy transfer (fret)
JP3085409B2 (en) * 1991-03-29 2000-09-11 東洋紡績株式会社 Method for detecting target nucleic acid sequence and reagent kit therefor
CA2074214C (en) * 1991-07-23 2000-02-22 Will Bloch Improvements in the in situ pcr
DE69233391D1 (en) * 1991-11-07 2004-09-02 Nanotronics Inc Hybridization of polynucleotides conjugated with chromophores and fluorophores to create a donor-donor energy transfer system
US5567583A (en) * 1991-12-16 1996-10-22 Biotronics Corporation Methods for reducing non-specific priming in DNA detection
US5348853A (en) * 1991-12-16 1994-09-20 Biotronics Corporation Method for reducing non-specific priming in DNA amplification
ES2082256T3 (en) * 1992-03-23 1996-03-16 Hoffmann La Roche DNA DETECTION METHOD.
EP0601889A2 (en) * 1992-12-10 1994-06-15 Maine Medical Center Research Institute Nucleic acid probes
US5593840A (en) * 1993-01-27 1997-01-14 Oncor, Inc. Amplification of nucleic acid sequences
WO1994017206A1 (en) * 1993-01-27 1994-08-04 Oncor, Inc. Method for amplifying nucleic acid sequences
US5422252A (en) * 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
EP2423322A1 (en) * 1993-11-12 2012-02-29 PHRI Properties, Inc. Hybridization probes for nucleic acid detection, universal stems, methods and kits
US5654419A (en) * 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US5547861A (en) * 1994-04-18 1996-08-20 Becton, Dickinson And Company Detection of nucleic acid amplification
NZ266724A (en) * 1994-05-23 1998-08-26 Biotronics Corp Method for detecting target nucleic acid and kit therefor; using primers with at least 5 consecutive nucleotides matching or blocking polymerase composition
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5691145A (en) * 1996-08-27 1997-11-25 Becton, Dickinson And Company Detection of nucleic acids using G-quartets
US5681145A (en) * 1996-10-30 1997-10-28 Itt Automotive Electrical Systems, Inc. Low-noise, high-efficiency fan assembly combining unequal blade spacing angles and unequal blade setting angles

Also Published As

Publication number Publication date
DE69734283T2 (en) 2006-07-06
CA2260973C (en) 2003-03-18
JP2001513623A (en) 2001-09-04
EP0912597A1 (en) 1999-05-06
EP0912597A4 (en) 2001-04-11
ATE305476T1 (en) 2005-10-15
JP3597869B2 (en) 2004-12-08
US6090552A (en) 2000-07-18
JP2003111599A (en) 2003-04-15
WO1998002449A1 (en) 1998-01-22
EP0912597B1 (en) 2005-09-28
ES2248852T3 (en) 2006-03-16
AU3728597A (en) 1998-02-09
US6117635A (en) 2000-09-12
DE69734283D1 (en) 2006-02-09

Similar Documents

Publication Publication Date Title
CA2260973A1 (en) Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
AU3481297A (en) Monitoring hybridization during pcr
CA2525482C (en) Improved system for multicolor real-time pcr comprising 3 to 6 pairs of forester resonance energy transfer (fret) probes
Sauer et al. Single molecule DNA sequencing in submicrometer channels: state of the art and future prospects
JP3795557B2 (en) Method for detecting degradation of oligonucleotide labeled with luminescent label
US6248526B1 (en) Labeled primer for use in and detection of target nucleic acids
US5728528A (en) Universal spacer/energy transfer dyes
EA007652B1 (en) Methods for detection and analysis of polynucleotides using light harvesting multichromophores
AU3901095A (en) Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
KR970028548A (en) Method for detecting fluorescence polarization of nucleic acid
AR001619A1 (en) Method for amplification of specific nucleic acid sequences
US10787700B2 (en) Methods for detecting multiple nucleic acids in a sample using reporter compounds and binding members thereof
US7223568B2 (en) Methods for determining nucleotide sequences of single nucleic acid molecules
WO2022264174A1 (en) A method to amplify and detect target nucleic acids with very high specificity and sensitivity
AUPP844899A0 (en) A method for detecting methylated nucleic acids
WO1999029905A3 (en) Method for detecting apoptosis using fret labeled oligonucleotides
WO2001011078A8 (en) Amplification method for detection of target nucleic acids involving fluorescence energy transfer
Saunders Real-time PCR
US20230279479A1 (en) Methods and systems for analyzing nucleic acids using increased ifret with multiple acceptor fluorophores
WO2022133137A1 (en) METHOD FOR RAPIDLY AND ACCURATELY DETECTING SARS-CoV-2 NUCLEIC ACID

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20170717