CA2261747A1 - Immunoassays in capillary tubes - Google Patents
Immunoassays in capillary tubesInfo
- Publication number
- CA2261747A1 CA2261747A1 CA002261747A CA2261747A CA2261747A1 CA 2261747 A1 CA2261747 A1 CA 2261747A1 CA 002261747 A CA002261747 A CA 002261747A CA 2261747 A CA2261747 A CA 2261747A CA 2261747 A1 CA2261747 A1 CA 2261747A1
- Authority
- CA
- Canada
- Prior art keywords
- analyte
- sample
- devices
- capillary tube
- capillary tubes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
- G01N33/552—Glass or silica
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N2021/0346—Capillary cells; Microcells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6482—Sample cells, cuvettes
Abstract
A fluorescent immunoassay employing the interior surface of a capillary tube is provided. Devices to permit immunoassays using one or more capillary tubes, an apparatus for use with the devices, and a process for screening for analyte in a sample using the devices and apparatus are also provided. Samples suspected of containing analyte are added to a disposable self-contained sample tray containing one or more sample wells, mixed with a reagent, drawn into one or more spaced-apart capillary tubes held within a disposable cartridge connected to an analytical apparatus, reacted with a binding member on the surface of the capillary tube, washed to stop the reaction, and dried by the apparatus. The capillary tube is then exposed to a signal generation device to create a fluorescence signal that is detected using a signal detector. The apparatus determines the presence of the analyte and optionally determines the amount of analyte present in the sample, and presents the results to the operator.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/688,043 | 1996-07-29 | ||
US08/688,043 US5976896A (en) | 1994-06-06 | 1996-07-29 | Immunoassays in capillary tubes |
PCT/US1997/012737 WO1998004920A2 (en) | 1996-07-29 | 1997-07-22 | Immunoassays in capillary tubes |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2261747A1 true CA2261747A1 (en) | 1998-02-05 |
CA2261747C CA2261747C (en) | 2005-07-12 |
Family
ID=24762887
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002261747A Expired - Fee Related CA2261747C (en) | 1996-07-29 | 1997-07-22 | Immunoassays in capillary tubes |
Country Status (9)
Country | Link |
---|---|
US (3) | US5976896A (en) |
EP (1) | EP0934524B1 (en) |
AT (1) | ATE262181T1 (en) |
AU (1) | AU732627B2 (en) |
BR (1) | BR9710887A (en) |
CA (1) | CA2261747C (en) |
DE (1) | DE69728158T2 (en) |
NZ (1) | NZ333841A (en) |
WO (1) | WO1998004920A2 (en) |
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-
1996
- 1996-07-29 US US08/688,043 patent/US5976896A/en not_active Expired - Lifetime
-
1997
- 1997-07-22 CA CA002261747A patent/CA2261747C/en not_active Expired - Fee Related
- 1997-07-22 NZ NZ333841A patent/NZ333841A/en not_active IP Right Cessation
- 1997-07-22 DE DE69728158T patent/DE69728158T2/en not_active Expired - Lifetime
- 1997-07-22 AU AU38070/97A patent/AU732627B2/en not_active Expired
- 1997-07-22 BR BR9710887A patent/BR9710887A/en not_active IP Right Cessation
- 1997-07-22 WO PCT/US1997/012737 patent/WO1998004920A2/en active IP Right Grant
- 1997-07-22 AT AT97935039T patent/ATE262181T1/en not_active IP Right Cessation
- 1997-07-22 EP EP97935039A patent/EP0934524B1/en not_active Expired - Lifetime
-
1999
- 1999-07-26 US US09/361,391 patent/US6517778B1/en not_active Expired - Fee Related
-
2001
- 2001-12-28 US US10/034,424 patent/US20020148728A1/en not_active Abandoned
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EP0934524B1 (en) | 2004-03-17 |
WO1998004920A2 (en) | 1998-02-05 |
AU3807097A (en) | 1998-02-20 |
US6517778B1 (en) | 2003-02-11 |
US5976896A (en) | 1999-11-02 |
DE69728158T2 (en) | 2004-10-28 |
US20020148728A1 (en) | 2002-10-17 |
BR9710887A (en) | 1999-08-17 |
WO1998004920A3 (en) | 1998-06-04 |
DE69728158D1 (en) | 2004-04-22 |
EP0934524A2 (en) | 1999-08-11 |
AU732627B2 (en) | 2001-04-26 |
ATE262181T1 (en) | 2004-04-15 |
NZ333841A (en) | 2000-08-25 |
CA2261747C (en) | 2005-07-12 |
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