CA2266543A1 - Methods and compositions for identifying receptor effectors - Google Patents

Methods and compositions for identifying receptor effectors

Info

Publication number
CA2266543A1
CA2266543A1 CA002266543A CA2266543A CA2266543A1 CA 2266543 A1 CA2266543 A1 CA 2266543A1 CA 002266543 A CA002266543 A CA 002266543A CA 2266543 A CA2266543 A CA 2266543A CA 2266543 A1 CA2266543 A1 CA 2266543A1
Authority
CA
Canada
Prior art keywords
identifying
cellular protein
screening
compounds
compositions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002266543A
Other languages
French (fr)
Other versions
CA2266543C (en
Inventor
Joshua Trueheart
Jeremy I. Paul
Hans A. Fuernkranz
Debra Nathan
Scott Holmes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cadus Pharmaceutical Corp
Original Assignee
Cadus Pharmaceutical Corporation
Joshua Trueheart
Jeremy I. Paul
Hans A. Fuernkranz
Debra Nathan
Scott Holmes
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cadus Pharmaceutical Corporation, Joshua Trueheart, Jeremy I. Paul, Hans A. Fuernkranz, Debra Nathan, Scott Holmes filed Critical Cadus Pharmaceutical Corporation
Publication of CA2266543A1 publication Critical patent/CA2266543A1/en
Application granted granted Critical
Publication of CA2266543C publication Critical patent/CA2266543C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Abstract

The present invention makes available a rapid, effective assay for screening and identifying pharmaceutically effective compounds that specifically interact with and modulate the activity of a cellular protein, e.g., a receptor or ion channel. The subject assay enables rapid screening of large numbers of compounds to identify those which act as an agonist or antagonist to the bioactivity of the cellular protein. The subject assay is particularly amenable for identifying surrogate ligands for receptors especially from small molecule or peptide libraries or from peptides produced by an autocrine system. Identification can be achieved via activation of a yeast signalling pathway, specially the yeast pheromone system pathway.
CA002266543A 1996-09-24 1997-09-24 Methods and compositions for identifying receptor effectors Expired - Fee Related CA2266543C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US71891096A 1996-09-24 1996-09-24
US08/718,910 1996-09-24
US85146997A 1997-05-05 1997-05-05
US08/851,469 1997-05-05
PCT/US1997/017159 WO1998013513A2 (en) 1996-09-24 1997-09-24 Methods and compositions for identifying receptor effectors

Publications (2)

Publication Number Publication Date
CA2266543A1 true CA2266543A1 (en) 1998-04-02
CA2266543C CA2266543C (en) 2003-12-09

Family

ID=27109999

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002266543A Expired - Fee Related CA2266543C (en) 1996-09-24 1997-09-24 Methods and compositions for identifying receptor effectors

Country Status (8)

Country Link
US (1) US6159705A (en)
EP (1) EP0929691B1 (en)
JP (1) JP2001501097A (en)
AT (1) ATE284972T1 (en)
AU (1) AU733007B2 (en)
CA (1) CA2266543C (en)
DE (1) DE69731960T2 (en)
WO (1) WO1998013513A2 (en)

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Also Published As

Publication number Publication date
JP2001501097A (en) 2001-01-30
EP0929691B1 (en) 2004-12-15
WO1998013513A3 (en) 1998-09-03
CA2266543C (en) 2003-12-09
US6159705A (en) 2000-12-12
AU733007B2 (en) 2001-05-03
AU4593097A (en) 1998-04-17
EP0929691A2 (en) 1999-07-21
ATE284972T1 (en) 2005-01-15
DE69731960T2 (en) 2005-12-15
DE69731960D1 (en) 2005-01-20
WO1998013513A2 (en) 1998-04-02

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