CA2270227A1 - Detection of antigens via oligonucleotide antibody conjugates - Google Patents

Detection of antigens via oligonucleotide antibody conjugates

Info

Publication number
CA2270227A1
CA2270227A1 CA002270227A CA2270227A CA2270227A1 CA 2270227 A1 CA2270227 A1 CA 2270227A1 CA 002270227 A CA002270227 A CA 002270227A CA 2270227 A CA2270227 A CA 2270227A CA 2270227 A1 CA2270227 A1 CA 2270227A1
Authority
CA
Canada
Prior art keywords
oligonucleotide
labeled
dendrimer
detection
antigen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002270227A
Other languages
French (fr)
Other versions
CA2270227C (en
Inventor
Thor W. Nilsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genisphere LLC
Original Assignee
Polyprobe, Inc.
Thor W. Nilsen
Genisphere Inc.
Cf Partners Acquisition Iii, Llc
Genisphere, Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Polyprobe, Inc., Thor W. Nilsen, Genisphere Inc., Cf Partners Acquisition Iii, Llc, Genisphere, Llc filed Critical Polyprobe, Inc.
Publication of CA2270227A1 publication Critical patent/CA2270227A1/en
Application granted granted Critical
Publication of CA2270227C publication Critical patent/CA2270227C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals

Abstract

The present invention provides a method of detecting antigens, which comprises immobilizing an antigen to a solid support and contacting the solid support with a means for hybridizing a labeled dendrimer to the antibody, through an oligonucleotide complexed thereto. A directly oligonucleotide labeled primary antibody or an oligonucleotide labeled secondary antibody may be employed, and a conventionally labeled dendrimer can subsequently be hybridized to the oligonucleotide through one or more of the outer arms of the dendrimer. The present invention offers the advantage over conventional methods of antigen detection by providing multiple label molecules per antigen, thereby enhancing the observed signal associated with the label.
CA002270227A 1996-10-29 1997-10-22 Detection of antigens via oligonucleotide antibody conjugates Expired - Fee Related CA2270227C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/738,641 1996-10-29
US08/738,641 US6117631A (en) 1996-10-29 1996-10-29 Detection of antigens via oligonucleotide antibody conjugates
PCT/US1997/019093 WO1998018488A1 (en) 1996-10-29 1997-10-22 Detection of antigens via oligonucleotide antibody conjugates

Publications (2)

Publication Number Publication Date
CA2270227A1 true CA2270227A1 (en) 1998-05-07
CA2270227C CA2270227C (en) 2005-02-08

Family

ID=24968855

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002270227A Expired - Fee Related CA2270227C (en) 1996-10-29 1997-10-22 Detection of antigens via oligonucleotide antibody conjugates

Country Status (9)

Country Link
US (2) US6117631A (en)
EP (1) EP0951297B1 (en)
JP (1) JP3786970B2 (en)
AT (1) ATE320819T1 (en)
AU (1) AU718804B2 (en)
CA (1) CA2270227C (en)
DE (1) DE69735532T2 (en)
ES (1) ES2260787T3 (en)
WO (1) WO1998018488A1 (en)

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Also Published As

Publication number Publication date
AU718804B2 (en) 2000-04-20
EP0951297B1 (en) 2006-03-22
CA2270227C (en) 2005-02-08
EP0951297A4 (en) 2002-01-02
US6117631A (en) 2000-09-12
WO1998018488A1 (en) 1998-05-07
EP0951297A1 (en) 1999-10-27
DE69735532T2 (en) 2006-11-23
ATE320819T1 (en) 2006-04-15
AU4914097A (en) 1998-05-22
JP2001503517A (en) 2001-03-13
JP3786970B2 (en) 2006-06-21
ES2260787T3 (en) 2006-11-01
DE69735532D1 (en) 2006-05-11
US6110687A (en) 2000-08-29

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