CA2283261A1 - Methods for identifying nucleic acid sequences encoding agents that affect cellular phenotypes - Google Patents

Methods for identifying nucleic acid sequences encoding agents that affect cellular phenotypes Download PDF

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Publication number
CA2283261A1
CA2283261A1 CA002283261A CA2283261A CA2283261A1 CA 2283261 A1 CA2283261 A1 CA 2283261A1 CA 002283261 A CA002283261 A CA 002283261A CA 2283261 A CA2283261 A CA 2283261A CA 2283261 A1 CA2283261 A1 CA 2283261A1
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Prior art keywords
expression
nucleic acid
methods
reporter
acid sequences
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Granted
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CA002283261A
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French (fr)
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CA2283261C (en
Inventor
Carl Alexander Kamb
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Deltagen Proteomics Inc
Original Assignee
Arcaris, Inc.
Poritz, Mark A.
Ventana Genetics, Inc.
Carl Alexander Kamb
Deltagen Proteomics, Inc.
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Application filed by Arcaris, Inc., Poritz, Mark A., Ventana Genetics, Inc., Carl Alexander Kamb, Deltagen Proteomics, Inc. filed Critical Arcaris, Inc.
Publication of CA2283261A1 publication Critical patent/CA2283261A1/en
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Publication of CA2283261C publication Critical patent/CA2283261C/en
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Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Abstract

Methods for identifying nucleic acid sequences that affect a cellular phenotype are disclosed. The method uses a reporter gene whose level of expression correlates with the phenotype in conjunction with a method or device for measuring the level of reporter expression. An expression library is introduced into the cells, and those cells exhibiting changes in reporter expression level are selected. Expression library inserts from the selected cells are isolated, thereby providing a sub-library enriched for sequences that affect the phenotype reflected by the reporter. Further rounds of sub-library introduction and cell selection may be carried out to provide additional enrichment. Sequences identified using this method may be used to ascertain the identity of additional molecules involved in generating the cellular phenotype.
CA002283261A 1997-03-04 1998-02-27 Methods for identifying nucleic acid sequences encoding agents that affect cellular phenotypes Expired - Fee Related CA2283261C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/812,994 US5955275A (en) 1997-02-14 1997-03-04 Methods for identifying nucleic acid sequences encoding agents that affect cellular phenotypes
US08/812,994 1997-03-04
PCT/US1998/004376 WO1998039483A1 (en) 1997-03-04 1998-02-27 Methods for identifying nucleic acid sequences encoding agents that affect cellular phenotypes

Publications (2)

Publication Number Publication Date
CA2283261A1 true CA2283261A1 (en) 1998-09-11
CA2283261C CA2283261C (en) 2002-07-02

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CA002283261A Expired - Fee Related CA2283261C (en) 1997-03-04 1998-02-27 Methods for identifying nucleic acid sequences encoding agents that affect cellular phenotypes

Country Status (11)

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US (3) US5955275A (en)
EP (1) EP0973943B1 (en)
JP (1) JP2001514510A (en)
AT (1) ATE224457T1 (en)
AU (1) AU745827B2 (en)
CA (1) CA2283261C (en)
DE (1) DE69808060T2 (en)
DK (1) DK0973943T3 (en)
IL (1) IL131583A0 (en)
NO (1) NO994290L (en)
WO (1) WO1998039483A1 (en)

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DE69808060D1 (en) 2002-10-24
ATE224457T1 (en) 2002-10-15
WO1998039483A1 (en) 1998-09-11
EP0973943B1 (en) 2002-09-18
NO994290D0 (en) 1999-09-03
IL131583A0 (en) 2001-01-28
AU745827B2 (en) 2002-04-11
JP2001514510A (en) 2001-09-11
EP0973943A1 (en) 2000-01-26
NO994290L (en) 1999-11-03
US6579675B2 (en) 2003-06-17
US5955275A (en) 1999-09-21
DK0973943T3 (en) 2003-01-27
US20020098503A1 (en) 2002-07-25
US20020018992A1 (en) 2002-02-14
DE69808060T2 (en) 2003-04-30
AU6543898A (en) 1998-09-22
CA2283261C (en) 2002-07-02

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