CA2300358A1 - Novel phytase - Google Patents

Novel phytase Download PDF

Info

Publication number
CA2300358A1
CA2300358A1 CA002300358A CA2300358A CA2300358A1 CA 2300358 A1 CA2300358 A1 CA 2300358A1 CA 002300358 A CA002300358 A CA 002300358A CA 2300358 A CA2300358 A CA 2300358A CA 2300358 A1 CA2300358 A1 CA 2300358A1
Authority
CA
Canada
Prior art keywords
enzyme
phytase
phytate
novel phytase
kilodaltons
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002300358A
Other languages
French (fr)
Other versions
CA2300358C (en
Inventor
Jay Short
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Enzymes LLC
Original Assignee
Diversa Corporation
Kretz, Keith
Jay Short
Verenium Corporation
Basf Enzymes Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diversa Corporation, Kretz, Keith, Jay Short, Verenium Corporation, Basf Enzymes Llc filed Critical Diversa Corporation
Priority to CA002565314A priority Critical patent/CA2565314A1/en
Publication of CA2300358A1 publication Critical patent/CA2300358A1/en
Application granted granted Critical
Publication of CA2300358C publication Critical patent/CA2300358C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/06—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/25—Removal of unwanted matter, e.g. deodorisation or detoxification using enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/03026—4-Phytase (3.1.3.26), i.e. 6-phytase

Abstract

The invention provides a purified phytate enzyme derived from Escherichia coli B. The enzyme has a molecular weight of about 47.1 kilodaltons and has phytase activity (SEQ ID NO:2). The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in animal feed.
CA002300358A 1997-08-13 1998-08-13 Novel phytase Expired - Lifetime CA2300358C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002565314A CA2565314A1 (en) 1997-08-13 1998-08-13 Novel phytase

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/910,798 US5876997A (en) 1997-08-13 1997-08-13 Phytase
US08/910,798 1997-08-13
PCT/US1998/016728 WO1999008539A1 (en) 1997-08-13 1998-08-13 Novel phytase

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA002565314A Division CA2565314A1 (en) 1997-08-13 1998-08-13 Novel phytase

Publications (2)

Publication Number Publication Date
CA2300358A1 true CA2300358A1 (en) 1999-02-25
CA2300358C CA2300358C (en) 2008-03-11

Family

ID=25429341

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002300358A Expired - Lifetime CA2300358C (en) 1997-08-13 1998-08-13 Novel phytase

Country Status (10)

Country Link
US (3) US5876997A (en)
EP (3) EP1003379B1 (en)
JP (2) JP4194750B2 (en)
AT (1) ATE377360T1 (en)
AU (1) AU735371C (en)
CA (1) CA2300358C (en)
DE (1) DE69838676T2 (en)
DK (1) DK1003379T3 (en)
ES (1) ES2296341T3 (en)
WO (1) WO1999008539A1 (en)

Families Citing this family (91)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
CA2276064C (en) 1997-01-17 2013-12-17 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US7148054B2 (en) * 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6183740B1 (en) 1997-08-13 2001-02-06 Diversa Corporation Recombinant bacterial phytases and uses thereof
US20040091968A1 (en) * 1997-08-13 2004-05-13 Short Jay M. Recombinant phytases and methods of making and using them
US7432097B2 (en) 1997-08-13 2008-10-07 Verenium Corporation Phytases, nucleic acids encoding them and methods of making and using them
US7078035B2 (en) 1997-08-13 2006-07-18 Diversa Corporation Phytases, nucleic acids encoding them and methods for making and using them
US6855365B2 (en) 1997-08-13 2005-02-15 Diversa Corporation Recombinant bacterial phytases and uses thereof
US6720014B1 (en) * 1997-08-13 2004-04-13 Diversa Corporation Phytase-containing foodstuffs and methods of making and using them
US7390619B1 (en) * 1998-02-11 2008-06-24 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines
US6541011B2 (en) 1998-02-11 2003-04-01 Maxygen, Inc. Antigen library immunization
BR9910174A (en) 1998-05-01 2001-03-06 Maxygen Inc Process for obtaining an optimized recombinant gene for pest resistance, library, and process for obtaining an organism that is pathogenic to a vegetable pest
US6451572B1 (en) 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
AU5482299A (en) * 1998-08-12 2000-03-06 Maxygen, Inc. Dna shuffling to produce herbicide selective crops
WO2000009682A1 (en) 1998-08-12 2000-02-24 Maxygen, Inc. Dna shuffling of monooxygenase genes for production of industrial chemicals
US20060242731A1 (en) * 1998-08-12 2006-10-26 Venkiteswaran Subramanian DNA shuffling to produce herbicide selective crops
EP1119616A2 (en) 1998-10-07 2001-08-01 Maxygen, Inc. Dna shuffling to produce nucleic acids for mycotoxin detoxification
WO2000028018A1 (en) 1998-11-10 2000-05-18 Maxygen, Inc. Modified adp-glucose pyrophosphorylase for improvement and optimization of plant phenotypes
US6438561B1 (en) * 1998-11-19 2002-08-20 Navigation Technologies Corp. Method and system for using real-time traffic broadcasts with navigation systems
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US20030054390A1 (en) * 1999-01-19 2003-03-20 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6917882B2 (en) * 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
AU3210200A (en) 1999-01-19 2000-08-01 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US20070065838A1 (en) * 1999-01-19 2007-03-22 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US7024312B1 (en) * 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
CA2361384A1 (en) 1999-02-11 2000-08-17 Sun Ai Raillard High throughput mass spectrometry
JP3399518B2 (en) * 1999-03-03 2003-04-21 インターナショナル・ビジネス・マシーンズ・コーポレーション Semiconductor structure and method of manufacturing the same
US6365377B1 (en) 1999-03-05 2002-04-02 Maxygen, Inc. Recombination of insertion modified nucleic acids
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
DK1165806T3 (en) * 1999-03-31 2005-12-05 Cornell Res Foundation Inc Phosphatases with enhanced phytase activity
WO2000064247A1 (en) * 1999-04-23 2000-11-02 University Of Guelph Transgenic animals expressing salivary proteins
US7115795B1 (en) 1999-04-23 2006-10-03 University Of Guelph Transgenic animals expressing salivary proteins
US6303766B1 (en) 1999-05-14 2001-10-16 Virginia Tech Intellectual Properties, Inc. Soybean phytase and nucleic acid encoding the same
WO2001004287A1 (en) * 1999-07-07 2001-01-18 Maxygen Aps A method for preparing modified polypeptides
US20040002474A1 (en) * 1999-10-07 2004-01-01 Maxygen Inc. IFN-alpha homologues
US7430477B2 (en) * 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6841370B1 (en) 1999-11-18 2005-01-11 Cornell Research Foundation, Inc. Site-directed mutagenesis of Escherichia coli phytase
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
US7115712B1 (en) * 1999-12-02 2006-10-03 Maxygen, Inc. Cytokine polypeptides
US20010039014A1 (en) * 2000-01-11 2001-11-08 Maxygen, Inc. Integrated systems and methods for diversity generation and screening
AU2001241939A1 (en) * 2000-02-28 2001-09-12 Maxygen, Inc. Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation
WO2001073000A2 (en) * 2000-03-24 2001-10-04 Maxygen, Inc. Methods for modulating cellular and organismal phenotypes
AU2007201983B2 (en) * 2000-05-25 2011-12-01 Basf Enzymes Llc Recombinant bacterial phytases and uses thereof
AU2001261649A1 (en) * 2000-05-25 2001-12-03 Diversa Corporation Dietary aids and methods of use thereof
EP1297146A2 (en) * 2000-06-23 2003-04-02 Maxygen, Inc. Novel chimeric promoters
WO2002000717A2 (en) 2000-06-23 2002-01-03 Maxygen, Inc. Novel co-stimulatory molecules
WO2002004629A2 (en) * 2000-07-07 2002-01-17 Maxygen, Inc. Molecular breeding of transposable elements
US6858422B2 (en) * 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
US20020102577A1 (en) * 2000-07-31 2002-08-01 Raillard Sun Ai Nucleotide incorporating enzymes
US20020132308A1 (en) * 2000-08-24 2002-09-19 Mpep @ Page 300-M Novel constructs and their use in metabolic pathway engineering
BRPI0213813B1 (en) * 2001-10-31 2018-11-21 Cornell Res Foundation Inc method for enhancing the nutritional value of a feed consumed by a monogastric animal and feed
TW200305430A (en) * 2001-12-28 2003-11-01 Syngenta Participations Ag Thermotolerant phytase for animal feed
TWI262083B (en) * 2001-12-28 2006-09-21 Syngenta Participations Ag Microbially-expressed thermotolerant phytase for animal feed
AU2003258157A1 (en) * 2002-08-12 2004-02-25 Genencor International, Inc. Mutant e. coli appa phytase enzymes
US7309505B2 (en) * 2002-09-13 2007-12-18 Cornell Research Foundation, Inc. Using mutations to improve Aspergillus phytases
US7086750B2 (en) * 2003-06-23 2006-08-08 Se-Kure Controls, Inc. System for generating a message
KR20060027404A (en) * 2003-07-07 2006-03-27 신젠타 파티서페이션즈 아게 Reagents, methods and kits for detecting feed enzymes
US7009067B2 (en) * 2003-07-21 2006-03-07 Ip-6 Research, Inc. Hexa-citrated phytate and process of preparation thereof
US20050202447A1 (en) * 2004-03-11 2005-09-15 Surmodics, Inc. Array print buffers
PL1804592T3 (en) 2004-09-27 2010-04-30 Novozymes As Enzyme granules
DE102004050410A1 (en) 2004-10-15 2006-06-08 Ab Enzymes Gmbh Polypeptide with phytase activity and this coding nucleotide sequence
US7658922B2 (en) 2005-06-24 2010-02-09 Ab Enzymes Gmbh Monoclonal antibodies, hybridoma cell lines, methods and kits for detecting phytase
US20090270479A1 (en) * 2005-07-12 2009-10-29 Antonio Giordano Genetic and Epigenetic Alterations In the Diagnosis and Treatment of Cancer
WO2007084769A2 (en) 2006-01-19 2007-07-26 Solazyme, Inc. Microalgae-derived compositions for improving the health and appearance of skin
US7919297B2 (en) * 2006-02-21 2011-04-05 Cornell Research Foundation, Inc. Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase
US8540984B2 (en) * 2006-08-03 2013-09-24 Cornell Research Foundation, Inc. Phytases with improved thermal stability
CN101505611B (en) 2006-08-07 2013-03-27 诺维信公司 Enzyme granules for animal feed
CN101500430B (en) 2006-08-07 2014-02-19 诺维信公司 Enzyme granules for animal feed
CN113789316A (en) 2006-09-21 2021-12-14 巴斯夫酶有限责任公司 Phytases, nucleic acids encoding them and methods of making and using them
US8192734B2 (en) 2007-07-09 2012-06-05 Cornell University Compositions and methods for bone strengthening
GB0922467D0 (en) 2009-04-24 2010-02-03 Danisco Feed supplement
CN102459303B (en) 2009-05-21 2016-06-29 巴斯夫酶有限责任公司 Phytase, the nucleic acid encoding them and preparation and their method of use
CN104125778B (en) 2012-02-07 2021-09-07 丹尼斯科美国公司 Phytase stability improvement with phytic acid and compositions comprising phytase and phytic acid
EP2812429B1 (en) 2012-02-07 2020-07-29 Danisco US Inc. Glycosylation as a stabilizer for phytase
GB201308828D0 (en) 2013-03-12 2013-07-03 Verenium Corp Phytase
GB201308843D0 (en) 2013-03-14 2013-07-03 Verenium Corp Phytase formulation
JP2016525354A (en) 2013-07-25 2016-08-25 ビーエーエスエフ エンザイムズ エルエルシー Phytase
WO2015197871A1 (en) 2014-06-27 2015-12-30 Dsm Ip Assets B.V. A method for improving the nutritional value of animal feed
US9528096B1 (en) * 2016-06-30 2016-12-27 Fornia Biosolutions, Inc. Phytases and uses thereof
US9605245B1 (en) 2016-06-30 2017-03-28 Fornia BioSoultions, Inc. Phytases and uses thereof
US10351832B2 (en) 2016-06-30 2019-07-16 Fornia Biosolutions, Inc. Phytases and uses thereof
MY194353A (en) 2018-05-08 2022-11-29 Locus Agriculture Ip Co Llc Microbe based products for enhancing plant root and immune health
CN113301809A (en) 2018-11-20 2021-08-24 杜邦营养生物科学有限公司 Engineered robust high Tm phytase clade polypeptides and fragments thereof
CN115915956A (en) 2020-02-28 2023-04-04 杜邦营养生物科学有限公司 Feed composition
CN113652372B (en) * 2021-08-30 2023-02-07 湖南师范大学 Lactococcus lactis and application thereof in preparation of phytase and animal feed
CN113812507A (en) * 2021-09-27 2021-12-21 长春大学 Method for preparing digestion-free soybean protein by taking high-temperature and low-temperature soybean meal as raw material

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5366736A (en) * 1989-02-16 1994-11-22 University Of Georgia Research Foundation, Inc. Vitamin D derivative feed compositions and methods of use
UA27702C2 (en) * 1989-09-27 2000-10-16 Гіст-Брокейдс Н.В. Fragment of genomic dna coding phytase aspergillus niger, fragment of cdna coding phytase aspergillus niger, recombinant plasmid dna for expression of phytase in aspergillus (variants), strain aspergillus producent of aspergillus (variants), process for praparation
KR0169913B1 (en) * 1996-03-14 1999-01-15 김은영 New strain bacillus sp.ds11 (kctc 0231bp)and new phytase produced from this
US5939303A (en) * 1996-06-14 1999-08-17 Her Majesty The Queen In Right Of Canada, As Represented By The Dept. Of Agriculture & Agri-Food Canada Phytases of ruminal microorganisms
KR100206453B1 (en) * 1997-03-27 1999-07-01 박원훈 A novel strain e coli jm83/pkp2 transformed with a novel plasmid and phytase produced from it

Also Published As

Publication number Publication date
DE69838676T2 (en) 2008-10-30
US6190897B1 (en) 2001-02-20
EP1600505A1 (en) 2005-11-30
JP4194750B2 (en) 2008-12-10
JP2008099701A (en) 2008-05-01
ES2296341T3 (en) 2008-04-16
EP1003379A4 (en) 2000-11-15
DK1003379T3 (en) 2008-03-25
AU735371B2 (en) 2001-07-05
EP1003379B1 (en) 2007-11-07
AU735371C (en) 2006-03-02
AU8904498A (en) 1999-03-08
ATE377360T1 (en) 2007-11-15
EP1811020A1 (en) 2007-07-25
JP2001514869A (en) 2001-09-18
CA2300358C (en) 2008-03-11
US6110719A (en) 2000-08-29
US5876997A (en) 1999-03-02
EP1003379A1 (en) 2000-05-31
DE69838676D1 (en) 2007-12-20
WO1999008539A1 (en) 1999-02-25

Similar Documents

Publication Publication Date Title
CA2300358A1 (en) Novel phytase
EP1892292A3 (en) Recombinant bacterial phytases and uses thereof
AU2077197A (en) Polypeptides having phytase activity and nucleic acids encoding same
NZ333128A (en) Engineered human DNase I with DNA hydrolytic activity at least 50 percent greater than native human DNase I
AU7908598A (en) Alkaline xyloglucanase
NZ504548A (en) Polypeptides and nucleic acids encoding human chordin
BR0010946A (en) Recombinant bacterial phytases and their uses
EP1209237A3 (en) Synthetic DNA sequence having enhanced insecticidal activity in maize
MY127754A (en) Plasmid autonomously replicable in coryneform bacteria.
EP1688500A3 (en) Overexpression of phytase genes in yeast systems
AU7873498A (en) Phytase polypeptides
CA2252493A1 (en) .beta.-galactoside - .alpha. 2,6-sialyltransferase gene
NZ512835A (en) Genes identified as required for proliferation in escherichia coli
EP1037999A4 (en) Porphorymonas gingivalis polypeptides and nucleotides
CA2281206A1 (en) Geranyl diphosphate synthase genes
CA2266421A1 (en) Genes coding for a protein having glycoside transfer activity
AU4218389A (en) Peptide compounds
IL137256A0 (en) Frozen food product
NZ515110A (en) Promoter
CA2253006A1 (en) .beta.-carotene hydroxylase gene
CA2302551A1 (en) .beta.-fructofuranosidase and its gene
EP1018559B8 (en) Phosphodiesterase enzymes
EP1476462A4 (en) Novel fungal lipase
EP0824150A3 (en) Plant promoter and utilization thereof
AU6659896A (en) Protein with plant protecting properties

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20180813