CA2303477A1 - Chimaeric adenoviruses - Google Patents

Chimaeric adenoviruses Download PDF

Info

Publication number
CA2303477A1
CA2303477A1 CA002303477A CA2303477A CA2303477A1 CA 2303477 A1 CA2303477 A1 CA 2303477A1 CA 002303477 A CA002303477 A CA 002303477A CA 2303477 A CA2303477 A CA 2303477A CA 2303477 A1 CA2303477 A1 CA 2303477A1
Authority
CA
Canada
Prior art keywords
adenovirus
chimaeric
serotype
adenoviruses
vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002303477A
Other languages
French (fr)
Other versions
CA2303477C (en
Inventor
Menzo Havenga
Ronald Vogels
Abraham Bout
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Janssen Vaccines and Prevention BV
Original Assignee
Introgene B.V.
Menzo Havenga
Ronald Vogels
Abraham Bout
Crucell Holland B.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Introgene B.V., Menzo Havenga, Ronald Vogels, Abraham Bout, Crucell Holland B.V. filed Critical Introgene B.V.
Publication of CA2303477A1 publication Critical patent/CA2303477A1/en
Application granted granted Critical
Publication of CA2303477C publication Critical patent/CA2303477C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10344—Chimeric viral vector comprising heterologous viral elements for production of another viral vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/20011—Rhabdoviridae
    • C12N2760/20111—Lyssavirus, e.g. rabies virus
    • C12N2760/20134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein

Abstract

The present invention provides methods and vector systems for the generation of chimaeric recombinant adenoviruses. These hybrid adenoviruses contain a genome that is derived from different adenovirus serotypes. In particular, novel hybrid adenoviruses are disclosed with improved properties for gene therapy purposes. These properties include:
a decreased sensitivity towards neutralizing antibodies, a modified host range, a change in the titer to which adenovirus can be grown, the ability to escape trapping in the liver upon in vivo systemic delivery, and absence or decreased infection of antigen presenting cells (APC) of the immune system, such as macrophages or dendritic cells. These chimaeric adenoviruses thus represent improved tools for gene therapy and vaccination since they overcome the limitations observed with the currently used serotype subgroup C adenoviruses.

Claims (10)

1. A chimaeric adenovirus comprising at least a part of a fiber protein of an adenovirus serotype providing the chimaeric virus with a desired host range and at least a part of a penton or hexon protein from another less antigenic adenovirus serotype resulting in a less antigenic chimaeric adenovirus.
2. A recombinant vector derived from an adenovirus comprising at least one ITR and a packaging signal having an insertion site for a nucleic acid sequence of interest, and further having an insertion site for functionallly inserting a gene encoding a penton and/or a hexon protein of a first serotype of adenovirus and having an insertion site for a gene encoding a fiber protein of a second adenovirus of a different serotype.
3. A recombinant vector according to claim 2 which is a plasmid.
4. A packaging cell for producing a chimaeric adenovirus according to claim 1, comprising in trans all elements necessary for adenovirus production not present on the adenoviral vector according to claim 2.
5. A kit of parts comprising a packaging cell according to claim 4 and a recombinant vector according to claim 2 or 3, whereby there is essentially no overlap leading to recombination resulting in the production of replication competent adenovirus between said cell and said vector.
6. A vector according to claim 2 or 3 where the insertion sites are different and preferably unique restriction sites.
7. A method for producing a chimaeric adenovirus having a desired host range and diminished antigenicity, comprising providing a vector according to claim 2, inserting into said vector at least a functional part of a penton or hexon protein derived from an adenovirus serotype having relatively low antigenicity, inserting at least a functional part of a fiber protein derived from an adenovirus serotype having the desired host range and transfecting said vector in a packaging cell according to claim 4 and allowing for production of chimaeric viral particles.
8. A method according to claim 7, wherein said reduced antigenicity is a diminished capability to raise neutralizing antibodies.
9. A chimaeric adenovirus according to claim 1, wherein the hexon, penton and/or fiber proteins are chimaeric proteins originating from different adenovirus serotypes.
10. A nucleic acid library comprising nucleic acid derived from different adenovirus serotypes.
CA2303477A 1998-07-08 1999-07-08 Chimaeric adenoviruses Expired - Fee Related CA2303477C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP98202297 1998-07-08
EP98202297.2 1998-07-08
PCT/NL1999/000436 WO2000003029A2 (en) 1998-07-08 1999-07-08 Chimaeric adenoviruses

Publications (2)

Publication Number Publication Date
CA2303477A1 true CA2303477A1 (en) 2000-01-20
CA2303477C CA2303477C (en) 2010-04-06

Family

ID=8233905

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2303477A Expired - Fee Related CA2303477C (en) 1998-07-08 1999-07-08 Chimaeric adenoviruses

Country Status (10)

Country Link
US (3) US20030017138A1 (en)
EP (1) EP0978566B1 (en)
JP (1) JP4472178B2 (en)
AT (1) ATE325200T1 (en)
AU (1) AU765276B2 (en)
CA (1) CA2303477C (en)
DE (1) DE69931112T2 (en)
ES (1) ES2263250T3 (en)
NZ (1) NZ503018A (en)
WO (1) WO2000003029A2 (en)

Families Citing this family (68)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE278794T1 (en) 1995-06-15 2004-10-15 Crucell Holland Bv PACKAGING SYSTEMS FOR HUMAN, HUMAN ADENOVIRUSES, FOR USE IN GENE THERAPY
US20030017138A1 (en) 1998-07-08 2003-01-23 Menzo Havenga Chimeric adenoviruses
US6929946B1 (en) 1998-11-20 2005-08-16 Crucell Holland B.V. Gene delivery vectors provided with a tissue tropism for smooth muscle cells, and/or endothelial cells
DE60039683D1 (en) * 1999-03-04 2008-09-11 Crucell Holland Bv USE OF AN ADENOVIRUS VECTOR FOR THE TRANSDUCTION OF SYNOVIAL CELLS
US6869936B1 (en) 1999-03-04 2005-03-22 Crucell Holland B.V. Means and methods for fibroblast-like or macrophage-like cell transduction
US6492169B1 (en) 1999-05-18 2002-12-10 Crucell Holland, B.V. Complementing cell lines
US20050232900A1 (en) * 1999-05-18 2005-10-20 Crucell Holland B.V. Serotype of adenovirus and uses thereof
US6913922B1 (en) 1999-05-18 2005-07-05 Crucell Holland B.V. Serotype of adenovirus and uses thereof
EP1067188A1 (en) * 1999-07-08 2001-01-10 Introgene B.V. Infection with chimaeric adenoviruses of cells negative for the adenovirus serotype 5 Coxsacki adenovirus receptor (CAR)
DE19956763A1 (en) * 1999-11-25 2001-06-13 Guenter Cichon Reducing interaction of adenoviral vectors with blood cells, useful for ex vivo gene therapy, by mutating the fiber-encoding gene in vectors or helper constructs, reduces toxicity
US6867022B1 (en) 2000-01-21 2005-03-15 Regents Of The University Of Michigan Replication deficient adenovirus vectors and methods of making and using them
EP1191105A1 (en) * 2000-09-25 2002-03-27 Galapagos Genomics B.V. Gene delivery vectors provided with a tissue tropism for T-lymphocytes
EP1157999A1 (en) * 2000-05-24 2001-11-28 Introgene B.V. Methods and means for enhancing skin transplantation using gene delivery vehicles having tropism for primary fibroblasts, as well as other uses thereof
AU6368901A (en) * 2000-05-31 2001-12-11 Univ Saskatchewan Modified bovine adenovirus having altered tropism
US7754201B2 (en) * 2000-06-02 2010-07-13 GenPhar, Inc Method of vaccination through serotype rotation
WO2002012523A2 (en) * 2000-08-10 2002-02-14 Crucell Holland B.V. Transduction of chondrocytes using adenoviral vectors
US7235233B2 (en) 2000-09-26 2007-06-26 Crucell Holland B.V. Serotype 5 adenoviral vectors with chimeric fibers for gene delivery in skeletal muscle cells or myoblasts
EP1195440A1 (en) * 2000-10-06 2002-04-10 Introgene B.V. Gene delivery vectors for stem cells
WO2002029073A2 (en) * 2000-10-06 2002-04-11 Crucell Holland B.V. Gene delivery vectors for stem cells
AU2002314705A1 (en) * 2001-01-09 2002-10-28 Beverly L. Davidson Adenovirus serotype 30 (ad30) fiber protein and uses thereof
US6635466B2 (en) * 2001-01-09 2003-10-21 University Of Iowa Research Foundation Adenovirus serotype 30 (Ad30)
CA2439185A1 (en) 2001-02-23 2002-09-06 Novartis Ag Vector constructs
JP2002325573A (en) * 2001-04-27 2002-11-12 Japan Science & Technology Corp Vector
EP1256803A1 (en) 2001-05-07 2002-11-13 Crucell Holland B.V. Methods for the identification of antiviral compounds
EP1409748B1 (en) * 2001-06-22 2011-10-26 The Trustees of The University of Pennsylvania Recombinant Adenoviruses comprising simian adenovirus proteins and uses thereof.
CA2852277C (en) 2001-11-21 2018-02-20 Guangping Gao Simian adenovirus nucleic acid and amino acid sequences, vectors containing same, and methods of use
AU2007201747B2 (en) * 2001-11-21 2010-06-03 The Trustees Of The University Of Pennsylvania Simian adenovirus nucleic acid and amino acid sequences, vectors containing same, and methods of use
EP1944043A1 (en) * 2001-11-21 2008-07-16 The Trustees of the University of Pennsylvania Simian adenovirus nucleic acid and amino acid sequences, vectors containing same, and methods of use
US20040002060A1 (en) * 2002-01-24 2004-01-01 Novartis Ag Fiber shaft modifications for efficient targeting
EA010828B1 (en) 2002-04-25 2008-12-30 Круселл Холланд Б.В. Recombinant adenovirus vectors and methods for the production thereof and use
SI1497440T1 (en) 2002-04-25 2009-02-28 Crucell Holland Bv Stable adenoviral vectors and methods for propagation thereof
CA2499385A1 (en) * 2002-09-20 2004-04-01 Crucell Holland B.V. Modified adenoviral vectors for use in vaccines and gene therapy
WO2004037294A2 (en) 2002-10-23 2004-05-06 Crucell Holland B.V. New settings for recombinant adenoviral-based vaccines
US20080153083A1 (en) 2003-10-23 2008-06-26 Crucell Holland B.V. Settings for recombinant adenoviral-based vaccines
EP2258850B1 (en) 2002-12-17 2013-07-17 Crucell Holland B.V. Recominant viral-based malaria vaccins
WO2004099422A2 (en) 2003-03-28 2004-11-18 The Scripps Research Institute Adenovirus particles with enhanced infectivity of dendritic cells and particles with decreased infectivity of hepatocytes
EP1639116A2 (en) * 2003-06-11 2006-03-29 The Scripps Research Institute Modified fiber proteins for efficient receptor binding
US7291498B2 (en) 2003-06-20 2007-11-06 The Trustees Of The University Of Pennsylvania Methods of generating chimeric adenoviruses and uses for such chimeric adenoviruses
EP1636370B1 (en) 2003-06-20 2014-04-16 The Trustees of The University of Pennsylvania Methods of generating chimeric adenoviruses and uses for such chimeric adenoviruses
US20070207461A1 (en) 2004-02-23 2007-09-06 Crucell Holland B.V. Virus Purification Methods
JP4787936B2 (en) 2004-05-26 2011-10-05 サイオクサス セラピューティクス リミティド Chimeric adenovirus for use in cancer treatment
WO2006040330A2 (en) * 2004-10-13 2006-04-20 Crucell Holland B.V. Improved adenoviral vectors and uses thereof
CN101155915B (en) 2005-04-11 2013-12-04 克鲁塞尔荷兰公司 Virus purification using ultrafiltration
CA2627638A1 (en) * 2005-10-28 2007-05-03 Michael James Mathis Conditionally replicating viruses and methods for cancer virotherapy
WO2007110409A1 (en) * 2006-03-27 2007-10-04 Crucell Holland B.V. Compositions comprising a recombinant adenovirus and an adjuvant
CN102203242B (en) 2008-11-03 2013-06-12 克鲁塞尔荷兰公司 Method for the production of adenoviral vectors
US8460920B2 (en) 2009-10-15 2013-06-11 Crucell Holland B.V. Method for the purification of adenovirus particles
ES2445713T3 (en) 2009-10-15 2014-03-04 Crucell Holland B.V. Process for adenovirus purification from high cell density cultures
KR101957616B1 (en) * 2010-08-16 2019-03-12 더 솔크 인스티튜트 포 바이올로지칼 스터디즈 Adenoviral assembly method
CA2808556A1 (en) 2010-09-20 2012-03-29 Crucell Holland B.V. Therapeutic vaccination against active tuberculosis
BR112014012681A8 (en) 2011-11-28 2017-06-20 Crucell Holland Bv polypeptide, designed to provide a polypeptide, nucleic acid, and immunogenic composition
EP2855669B1 (en) * 2012-05-29 2018-10-10 GenVec, Inc. Modified serotype 28 adenoviral vectors
KR102089121B1 (en) 2013-03-14 2020-03-13 더 솔크 인스티튜트 포 바이올로지칼 스터디즈 Oncolytic adenovirus compositions
JP6013654B2 (en) 2013-09-19 2016-10-25 クルセル ホランド ベー ヴェー Improved adenovirus formulation
US9987314B2 (en) 2013-10-25 2018-06-05 Psioxus Therapeutics Limited Oncolytic adenoviruses armed with heterologous genes
ES2780366T3 (en) 2015-04-30 2020-08-25 Psioxus Therapeutics Ltd Oncolytic adenovirus encoding a b7 protein
US20170067028A1 (en) * 2015-05-15 2017-03-09 Douglas J. Ballon Radiolabeling of adeno associated virus
JP6487604B2 (en) 2015-10-06 2019-03-20 ヤンセン ファッシンズ アンド プリベンション ベーフェーＪａｎｓｓｅｎ Ｖａｃｃｉｎｅｓ ＆ Ｐｒｅｖｅｎｔｉｏｎ Ｂ．Ｖ． Methods for preventing plastic-induced degradation of biological agents
CN108697746A (en) 2015-12-17 2018-10-23 皮斯奥克斯治疗公司 Encode the virus of anti-tcr recombinant antibody or segment
CN117384961A (en) 2016-02-23 2024-01-12 萨克生物研究学院 Treatment of exogenous gene expression in adenoviruses with minimal effect on viral kinetics
AU2017222568B2 (en) 2016-02-23 2020-09-10 Salk Institute For Biological Studies High throughput assay for measuring adenovirus replication kinetics
GB201713765D0 (en) 2017-08-28 2017-10-11 Psioxus Therapeutics Ltd Modified adenovirus
CA3045892A1 (en) 2016-12-12 2018-06-21 Salk Institute For Biological Studies Tumor-targeting synthetic adenoviruses and uses thereof
CA3060573A1 (en) 2017-04-21 2018-10-25 Baylor College Of Medicine Oncolytic virotherapy and immunotherapy
EP3630987A4 (en) 2017-05-26 2021-03-24 EpicentRx, Inc. Recombinant adenoviruses carrying transgenes
GB202013940D0 (en) 2020-09-04 2020-10-21 Synpromics Ltd Regulatory nucleic acid sequences
JP2023545731A (en) 2020-10-07 2023-10-31 アスクレピオス バイオファーマシューティカル， インコーポレイテッド Therapeutic adeno-associated virus delivery of fukutin-related protein (FKRP) to treat dystroglycanopathy disorders including limb girdle type 2I (LGMD2I)
EP4338727A1 (en) 2022-09-14 2024-03-20 Roquette Freres Adenovirus formulations

Family Cites Families (97)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4593002A (en) * 1982-01-11 1986-06-03 Salk Institute Biotechnology/Industrial Associates, Inc. Viruses with recombinant surface proteins
US4487829A (en) * 1982-03-23 1984-12-11 Massachusetts Institute Of Technology Production and use of monoclonal antibodies against adenoviruses
US4517686A (en) * 1982-08-04 1985-05-21 La Jolla Cancer Research Foundation Polypeptide
US4589881A (en) * 1982-08-04 1986-05-20 La Jolla Cancer Research Foundation Polypeptide
US4578079A (en) * 1982-08-04 1986-03-25 La Jolla Cancer Research Foundation Tetrapeptide
US4792525A (en) * 1982-08-04 1988-12-20 La Jolla Cancer Research Foundation Tetrapeptide
US4797368A (en) * 1985-03-15 1989-01-10 The United States Of America As Represented By The Department Of Health And Human Services Adeno-associated virus as eukaryotic expression vector
FR2602790B1 (en) 1986-08-13 1990-06-01 Transgene Sa EXPRESSION OF A SPECIFIC TUMOR ANTIGEN BY A RECOMBINANT VECTOR VIRUS AND USE THEREOF FOR THE PREVENTIVE OR CURATIVE TREATMENT OF THE CORRESPONDING TUMOR
US5166320A (en) * 1987-04-22 1992-11-24 University Of Connecticut Carrier system and method for the introduction of genes into mammalian cells
US4956281A (en) * 1987-06-03 1990-09-11 Biogen, Inc. DNA sequences, recombinant DNA molecules and processes for producing lymphocyte function associated antigen-3
US5024939A (en) * 1987-07-09 1991-06-18 Genentech, Inc. Transient expression system for producing recombinant protein
US5585254A (en) * 1987-08-21 1996-12-17 University Of Colorado Foundation, Inc. Autonomous parvovirus gene delivery vehicles and expression vectors
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
CA2000048A1 (en) * 1988-10-03 1990-04-03 Edward F. Plow Peptides and antibodies that inhibit integrin-ligand bindin g
US5198346A (en) * 1989-01-06 1993-03-30 Protein Engineering Corp. Generation and selection of novel DNA-binding proteins and polypeptides
US5096815A (en) * 1989-01-06 1992-03-17 Protein Engineering Corporation Generation and selection of novel dna-binding proteins and polypeptides
ATE219519T1 (en) * 1989-01-23 2002-07-15 Chiron Corp RECOMBINANT THERAPIES FOR INFECTIONS AND HYPERPROLIFERATIVE DISORDERS
US5223394A (en) * 1989-04-10 1993-06-29 Biogen, Inc. Recombinant dna molecule comprising lymphocyte function-associated antigen 3 phosphatidylinositol linkage signal sequence
ES2096590T3 (en) 1989-06-29 1997-03-16 Medarex Inc BI-SPECIFIC REAGENTS FOR AIDS THERAPY.
US5240846A (en) * 1989-08-22 1993-08-31 The Regents Of The University Of Michigan Gene therapy vector for cystic fibrosis
US5332567A (en) * 1989-08-24 1994-07-26 Immunomedics Detection and treatment of infections with immunoconjugates
US5436146A (en) * 1989-09-07 1995-07-25 The Trustees Of Princeton University Helper-free stocks of recombinant adeno-associated virus vectors
JPH05504677A (en) 1989-10-20 1993-07-22 トラスティーズ オブ ダートマス カレッジ Monoclonal antibody specific for IgA receptor
WO1991005871A1 (en) 1989-10-20 1991-05-02 Medarex, Inc. Bispecific heteroantibodies with dual effector functions
WO1991018088A1 (en) * 1990-05-23 1991-11-28 The United States Of America, Represented By The Secretary, United States Department Of Commerce Adeno-associated virus (aav)-based eucaryotic vectors
US5349053A (en) * 1990-06-01 1994-09-20 Protein Design Labs, Inc. Chimeric ligand/immunoglobulin molecules and their uses
US5246921A (en) * 1990-06-26 1993-09-21 The Wistar Institute Of Anatomy And Biology Method for treating leukemias
GB2246779B (en) 1990-08-03 1994-08-17 Delta Biotechnology Ltd Tumour-associated protease inhibitors targeted to tumour cells
GB9101550D0 (en) 1991-01-24 1991-03-06 Mastico Robert A Antigen-presenting chimaeric protein
JP3534749B2 (en) 1991-08-20 2004-06-07 アメリカ合衆国 Adenovirus-mediated gene transfer into the gastrointestinal tract
FR2681786A1 (en) 1991-09-27 1993-04-02 Centre Nat Rech Scient RECOMBINANT VECTORS OF VIRAL ORIGIN, PROCESS FOR OBTAINING SAME AND THEIR USE FOR THE EXPRESSION OF POLYPEPTIDES IN MUSCLE CELLS.
IL103059A0 (en) 1991-09-30 1993-02-21 Boehringer Ingelheim Int Conjugates for introducing nucleic acid into higher eucaryotic cells
NZ244306A (en) 1991-09-30 1995-07-26 Boehringer Ingelheim Int Composition for introducing nucleic acid complexes into eucaryotic cells, complex containing nucleic acid and endosomolytic agent, peptide with endosomolytic domain and nucleic acid binding domain and preparation
US5521291A (en) * 1991-09-30 1996-05-28 Boehringer Ingelheim International, Gmbh Conjugates for introducing nucleic acid into higher eucaryotic cells
JPH073958B2 (en) * 1992-01-31 1995-01-18 インターナショナル・ビジネス・マシーンズ・コーポレイション Termination circuit
ATE404683T1 (en) 1992-09-25 2008-08-15 Aventis Pharma Sa ADENOVIRUS VECTORS FOR TRANSFER OF FOREIGN GENES INTO CELLS OF THE CENTRAL NERVOUS SYSTEM, ESPECIALLY IN THE BRAIN
GB9223084D0 (en) 1992-11-04 1992-12-16 Imp Cancer Res Tech Compounds to target cells
DE69333886T2 (en) 1992-11-18 2006-07-27 Arch Development Corp., Chicago Adenovirus-guided gene transfer to the heart and smooth vascular muscle
GB9300686D0 (en) 1993-01-15 1993-03-03 Imp Cancer Res Tech Compounds for targeting
AU6133394A (en) 1993-02-09 1994-08-29 Scripps Research Institute, The Targeting and delivery of genes and antiviral agents into cells by the adenovirus penton
DE4311651A1 (en) 1993-04-08 1994-10-13 Boehringer Ingelheim Int Virus for the transport of foreign DNA into higher eukaryotic cells
PT698109E (en) 1993-05-10 2002-12-31 Univ Michigan TRANSFER OF GENES INTO PANCREATIC EPITHELIAL CELLS
US5543328A (en) * 1993-08-13 1996-08-06 Genetic Therapy, Inc. Adenoviruses having modified fiber proteins
US5631236A (en) * 1993-08-26 1997-05-20 Baylor College Of Medicine Gene therapy for solid tumors, using a DNA sequence encoding HSV-Tk or VZV-Tk
EP0716711A1 (en) 1993-09-03 1996-06-19 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Vector for gene therapy of the liver
US5552311A (en) * 1993-09-14 1996-09-03 University Of Alabama At Birmingham Research Foundation Purine nucleoside phosphorylase gene therapy for human malignancy
US5534423A (en) * 1993-10-08 1996-07-09 Regents Of The University Of Michigan Methods of increasing rates of infection by directing motion of vectors
FR2712812B1 (en) 1993-11-23 1996-02-09 Centre Nat Rech Scient Composition for the production of therapeutic products in vivo.
IT1271461B (en) 1993-12-01 1997-05-28 Menarini Ricerche Sud Spa ANTI-CD3 / ANTI-EGFR MONOCLONAL ANTI-BODY ANTIBODY, PROCESS FOR PRODUCTION AND ITS USE.
US5443953A (en) * 1993-12-08 1995-08-22 Immunomedics, Inc. Preparation and use of immunoconjugates
US5928944A (en) 1994-02-04 1999-07-27 The United States Of America As Represented By The Department Of Health And Human Services Method of adenoviral-medicated cell transfection
US6312699B1 (en) 1994-03-28 2001-11-06 Uab Research Foundation Ligands added to adenovirus fiber
US7252989B1 (en) 1994-04-04 2007-08-07 Board Of Regents, The University Of Texas System Adenovirus supervector system
DE69520496T2 (en) 1994-05-13 2001-07-12 Chiron Corp METHOD AND COMPOSITIONS AS VEHICLES FOR TARGETED INTRODUCTION OF GENES
US5560905A (en) * 1994-05-13 1996-10-01 The Proctor & Gamble Company Oral compositions
US5571531A (en) 1994-05-18 1996-11-05 Mcmaster University Microparticle delivery system with a functionalized silicone bonded to the matrix
US5570975A (en) 1994-06-27 1996-11-05 Reinert, Sr.; Gary L. Metal foundation push-it and installation apparatus and method
FR2721943B1 (en) 1994-06-29 1996-08-02 Rhone Poulenc Rorer Sa ADENOVIRUS COMPRISING A GENE ENCODING A SUPEROXIDE DISMUTASE
US5846782A (en) * 1995-11-28 1998-12-08 Genvec, Inc. Targeting adenovirus with use of constrained peptide motifs
US5559099A (en) * 1994-09-08 1996-09-24 Genvec, Inc. Penton base protein and methods of using same
AU705595B2 (en) 1994-09-09 1999-05-27 Neurocrine Biosciences, Incorporated Interleukin-1 type 3 receptors
FR2724846B1 (en) 1994-09-27 1996-12-20 Rhone Poulenc Rorer Sa METHOD OF TREATING CANCER BY REGULATING THE ACTIVITY OF RAS PROTEINS
FR2725726B1 (en) 1994-10-17 1997-01-03 Centre Nat Rech Scient VIRAL VECTORS AND USE IN GENE THERAPY
DK0787200T3 (en) * 1994-10-28 2005-08-15 Univ Pennsylvania Improved adenovirus and methods for its use
US5856152A (en) * 1994-10-28 1999-01-05 The Trustees Of The University Of Pennsylvania Hybrid adenovirus-AAV vector and methods of use therefor
WO1996014837A1 (en) 1994-11-09 1996-05-23 Genetic Therapy, Inc. Gene therapy for hypercholesterolemia
AU3993495A (en) 1994-11-29 1996-06-19 Takara Shuzo Co., Ltd. Process for producing transformed cell
FR2727867B1 (en) 1994-12-13 1997-01-31 Rhone Poulenc Rorer Sa GENE TRANSFER IN MEDULLAR MOTONURONES USING ADENOVIRAL VECTORS
AUPN107195A0 (en) 1995-02-10 1995-03-09 Withers, Graham Rex Metal matrix forming method and apparatus
US6127525A (en) * 1995-02-21 2000-10-03 Cornell Research Foundation, Inc. Chimeric adenoviral coat protein and methods of using same
US5770442A (en) * 1995-02-21 1998-06-23 Cornell Research Foundation, Inc. Chimeric adenoviral fiber protein and methods of using same
EP0835320A1 (en) 1995-05-10 1998-04-15 Introgene B.V. Improved retroviral vectors, especially suitable for gene therapy
ATE278794T1 (en) 1995-06-15 2004-10-15 Crucell Holland Bv PACKAGING SYSTEMS FOR HUMAN, HUMAN ADENOVIRUSES, FOR USE IN GENE THERAPY
US5622699A (en) * 1995-09-11 1997-04-22 La Jolla Cancer Research Foundation Method of identifying molecules that home to a selected organ in vivo
US5837511A (en) * 1995-10-02 1998-11-17 Cornell Research Foundation, Inc. Non-group C adenoviral vectors
SK72298A3 (en) * 1995-11-28 1998-12-02 Genvec Inc Vectors and methods for gene transfer to cells
EP0866721A4 (en) 1995-12-08 2003-06-04 Univ Alabama Birmingham Res Fo Targeted adenovirus vectors
CA2251738A1 (en) 1996-04-16 1997-10-23 Immusol Incorporated Targeted viral vectors
US5877011A (en) * 1996-11-20 1999-03-02 Genzyme Corporation Chimeric adenoviral vectors
US5922315A (en) * 1997-01-24 1999-07-13 Genetic Therapy, Inc. Adenoviruses having altered hexon proteins
DE69830320T2 (en) * 1997-03-07 2006-02-02 The Wistar Institute Of Anatomy And Biology Use of adenoviral vectors expressing PDGF or VEGF for the healing of tissue defects and for the induction of hypervascularism in mastoid tissues
US6100086A (en) * 1997-04-14 2000-08-08 Genzyme Corporation Transgene expression systems
NZ500791A (en) * 1997-05-08 2002-10-25 Genetic Therapy Inc Gene transfer with adenoviruses having modified fiber proteins
US5849561A (en) * 1997-05-22 1998-12-15 Cornell Research Foundation, Inc. Method for the production of non-group C adenoviral vectors
US6287857B1 (en) * 1998-02-09 2001-09-11 Genzyme Corporation Nucleic acid delivery vehicles
AU3358999A (en) 1998-03-20 1999-10-11 Genzyme Corporation Chimeric adenoviral vectors for targeted gene delivery
US20030017138A1 (en) 1998-07-08 2003-01-23 Menzo Havenga Chimeric adenoviruses
AU767975B2 (en) * 1998-09-11 2003-11-27 Genvec, Inc. Alternatively targeted adenovirus
EP1020529B1 (en) 1998-11-20 2005-06-01 Crucell Holland B.V. Gene delivery vectors provided with a tissue tropism for smooth muscle cells, and/or endothelial cells
EP1016726A1 (en) 1998-12-30 2000-07-05 Introgene B.V. Gene therapy to promote angiogenesis
US6869936B1 (en) * 1999-03-04 2005-03-22 Crucell Holland B.V. Means and methods for fibroblast-like or macrophage-like cell transduction
EP1550722B1 (en) 1999-05-17 2007-06-13 Crucell Holland B.V. Recombinant human adenovirus serotype 35
US6492169B1 (en) * 1999-05-18 2002-12-10 Crucell Holland, B.V. Complementing cell lines
EP1067188A1 (en) 1999-07-08 2001-01-10 Introgene B.V. Infection with chimaeric adenoviruses of cells negative for the adenovirus serotype 5 Coxsacki adenovirus receptor (CAR)
EP1157999A1 (en) 2000-05-24 2001-11-28 Introgene B.V. Methods and means for enhancing skin transplantation using gene delivery vehicles having tropism for primary fibroblasts, as well as other uses thereof
WO2002012523A2 (en) * 2000-08-10 2002-02-14 Crucell Holland B.V. Transduction of chondrocytes using adenoviral vectors
US6905678B2 (en) * 2001-07-07 2005-06-14 Crucell Holland B.V. Gene delivery vectors with cell type specificity for mesenchymal stem cells

Also Published As

Publication number Publication date
WO2000003029A2 (en) 2000-01-20
EP0978566A2 (en) 2000-02-09
EP0978566A3 (en) 2000-04-19
AU765276B2 (en) 2003-09-11
CA2303477C (en) 2010-04-06
US20030017138A1 (en) 2003-01-23
DE69931112T2 (en) 2006-12-07
EP0978566B1 (en) 2006-05-03
JP2002520026A (en) 2002-07-09
ATE325200T1 (en) 2006-06-15
US7749493B2 (en) 2010-07-06
US20030073072A1 (en) 2003-04-17
JP4472178B2 (en) 2010-06-02
AU4935699A (en) 2000-02-01
US20060014276A1 (en) 2006-01-19
ES2263250T3 (en) 2006-12-01
WO2000003029A3 (en) 2000-03-16
NZ503018A (en) 2003-06-30
DE69931112D1 (en) 2006-06-08

Similar Documents

Publication Publication Date Title
CA2303477A1 (en) Chimaeric adenoviruses
Croyle et al. PEGylation of E1-deleted adenovirus vectors allows significant gene expression on readministration to liver
Reddy et al. Replication-defective bovine adenovirus type 3 as an expression vector
Appaiahgari et al. Adenoviruses as gene/vaccine delivery vectors: promises and pitfalls
AU696495B2 (en) Adenoviral vectors of animal origin and use in gene therapy
CA2074502C (en) Vaccines
Randrianarison-Jewtoukoff et al. Recombinant adenoviruses as vaccines
US20240018546A1 (en) Single cycle replicating adenovirus vectors
EP0979101A4 (en) Recombinant canine adenovirus (cav) containing exogenous dna
EP0766569A4 (en) Aav capsid vehicles for molecular transfer
HU195619B (en) Process for producing oral vaccines against hepatitis b virus
KR20220097440A (en) Viruses with modified capsid proteins
WO2001055362A3 (en) Hybrid adeno-retroviral vector for the transfection of cells
CA2461579A1 (en) Porcine adenovirus e1 region
CA2410665C (en) Modified bovine adenovirus having altered tropism
ES2748468T3 (en) Recombinant swinepox virus and vaccines
CA2448908A1 (en) Adenovirus protein ix, its domains involved in capsid assembly, transcriptional activity and nuclear reorganization
CA2325574A1 (en) Porcine adenovirus type 3 genome
AU8097698A (en) Bovine adenovirus type 3 genome
CA2527721C (en) Chimeric adenovirus capsid proteins
JP2000157289A5 (en)
Scholz et al. Transactivation of the early SV40 promoter by avian infectious laryngotracheitis virus in avian hepatoma cells
US20110123569A1 (en) Vaccine directed against adenovirus serotype 14
US7264818B2 (en) BAV packaging regions and E1 transcriptional control regions
EP1026252A1 (en) Synthetic gene of bovine viral diarrhoea virus

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20190708