CA2307842A1 - Hyaluronan synthase gene and uses thereof - Google Patents

Hyaluronan synthase gene and uses thereof Download PDF

Info

Publication number
CA2307842A1
CA2307842A1 CA002307842A CA2307842A CA2307842A1 CA 2307842 A1 CA2307842 A1 CA 2307842A1 CA 002307842 A CA002307842 A CA 002307842A CA 2307842 A CA2307842 A CA 2307842A CA 2307842 A1 CA2307842 A1 CA 2307842A1
Authority
CA
Canada
Prior art keywords
nucleic acid
seq
host cell
acid segment
hyaluronan synthase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002307842A
Other languages
French (fr)
Other versions
CA2307842C (en
Inventor
Paul H. Weigel
Kshama Kumari
Paul Deangelis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Oklahoma
Original Assignee
The Board Of Regents Of The University Of Oklahoma
Paul H. Weigel
Kshama Kumari
Paul Deangelis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Board Of Regents Of The University Of Oklahoma, Paul H. Weigel, Kshama Kumari, Paul Deangelis filed Critical The Board Of Regents Of The University Of Oklahoma
Publication of CA2307842A1 publication Critical patent/CA2307842A1/en
Application granted granted Critical
Publication of CA2307842C publication Critical patent/CA2307842C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/102—Pasteurellales, e.g. Actinobacillus, Pasteurella; Haemophilus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
    • C08B37/0072—Hyaluronic acid, i.e. HA or hyaluronan; Derivatives thereof, e.g. crosslinked hyaluronic acid (hylan) or hyaluronates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y10S435/836—Bacillus licheniformis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y10S435/839—Bacillus subtilis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia

Abstract

The present invention relates to a nucleic acid segment having a coding region segment encoding enzymatically active Streptococcus equisimilis hyaluronate synthase (seHAS), and to the use of this nucleic acid segment in the preparation of recombinant cells which produce hyaluronate synthase and its hyaluronic acid product. Hyaluronate is also known as hyaluronic acid or hyaluronan.

Claims (59)

1. A purified nucleic acid segment comprising a coding region encoding enzymatically active hyaluronate synthase.
2. The purified nucleic acid segment of claim 1, wherein the purified nucleic acid segment encodes the Streptococcus equisimilis hyaluronate synthase of SEQ ID NO:2.
3. The purified nucleic acid segment of claim 1, wherein the purified nucleic acid segment comprises a nucleotide sequence in accordance with SEQ ID NO:1.
4. A purified nucleic acid segment having a coding region encoding enzymatically active hyaluronate synthase, Wherein the purified nucleic acid segment is capable of hybridizing to the nucleotide sequence of SEQ ID NO:1.
5. A purified nucleic acid segment having a coding region encoding enzymatically active hyaluronate synthase, wherein the purified nucleic acid segment has semiconservative or conservative amino codon acid changes when compared to the nucleotide sequence of SEQ ID NO:1.
6. A recombinant vector selected from the group consisting of a plasmid, cosmid, phage, or virus vector and wherein the recombinant vector further comprises a purified nucleic acid segment having a coding region encoding enzymatically active hyaluronan synthase.
7. The recombinant vector of claim 5, Wherein the purified nucleic acid segment encodes the Streptococcus equison hyaluronan synthase of SEQ ID NO:2.
8. The recombinant vector of claim 6, wherein the purified nucleic acid segment comprises a nucleotide sequence in accordance with SEQ ID NO: 1.
9. The recombinant vector of claim 6, wherein the plasmid further comprises an expression vector.
10. The recombinant vector of claim 9, wherein the expression vector comprises a promoter operatively linked to the enzymatically active Streptococcus equisimilis hyaluronan synthase coding region.
11. A recombinant host cell, wherein the recombinant host cell is a prokaryotic cell transformed with a recombinant vector comprising a purified nucleic acid segment having a coding region encoding enzymatically active hyaluronan synthase.
12. The recombinant host cell of claim 11, wherein the purified nucleic acid segment encodes the Streptococcus equisimilis hyaluronan synthase of SEQ ID NO:2.
13. The recombinant host cell of claim 11, Wherein the purified nucleic acid segment comprises a nucleotide sequence in accordance with SEQ ID NO:1.
14. The recombinant host cell of claim 13, wherein the host cell produces hyaluronic acid.
15. The recombinant host cell of claim 11, wherein the enzymatically active hyaluronan synthase is capable of producing a hyaluronic acid polymer having a modified structure.
16. The recombinant host cell of claim 11, wherein the enzymatically active hyaluronan synthase is capable of producing a hyaluronic acid polymer having a modified size distribution.
17. A recombinant host cell, Wherein the recombinant host cell is a eukaryotic cell transfected with a recombinant vector comprising a purified nucleic acid segment having a coding region encoding enzymatically active hyaluronan synthase.
18. The recombinant host cell of claim 17, Wherein the purified nucleic acid segment encodes the Streptococcus equisimilis hyaluronan synthase of SEQ ID NO:2.
19. The recombinant host cell of claim 17, wherein the purified nucleic acid segment comprises a nucleotide sequence in accordance with SEQ ID NO:1.
20. The recombinant host cell of claim 19, wherein the host cell produces hyaluronic acid.
21. The recombinant host cell of claim 17, wherein the enzymatically active hyaluronan synthase is capable of producing a hyaluronic acid polymer having a modified structure.
22. The recombinant host cell of claim 17, wherein the enzymatically active hyaluronan synthase is capable of producing a hyaluronic acid polymer having a modified size distribution.
23. A recombinant host cell, wherein the recombinant host cell is electroporated to introduce a recombinant vector into the recombinant host cell, wherein the recombinant vector comprises a purified nucleic acid segment having a coding region encoding enzymatically active hyaluronan synthase.
24. The recombinant host cell of claim 23, wherein the purified nucleic acid segment encodes the Streptococcus equisimilis hyaluronan synthase of SEQ ID NO:2.
25. The recombinant host cell of claim 23, wherein the purified nucleic acid segment comprises a nucleotide sequence in accordance with SEQ ID NO:1.
26. The recombinant host cell of claim 25, wherein the host cell produces hyaluronic acid.
27. The recombinant host cell of claim 23, wherein the enzymatically active hyaluronan synthase is capable of producing a hyaluronic acid polymer having a modified structure.
28. The recombinant host cell of claim 23, wherein the enzymatically active Streptococcus equisimilis hyaluronan synthase is capable of producing a hyaluronic acid polymer having a modified size distribution.
29. A recombinant host cell, wherein the recombinant host cell is transduced with a recombinant vector comprising a purified nucleic acid segment having a coding region encoding enzymatically active Streptococcus equisimilis hyaluronan synthase.
30. The recombinant host cell of claim 29, wherein the purified nucleic acid segment encodes the Streptococcus equisimilis hyaluronan synthase of SEQ ID NO:2.
31. The recombinant host cell of claim 29, wherein the purified nucleic acid segment comprises a nucleotide sequence in accordance with SEQ ID NO:1.
32. The recombinant host cell of claim 31, wherein the host cell produces hyaluronic acid.
33. The recombinant host cell of claim 29, Wherein the enzymatically active hyaluronan synthase is capable of producing a hyaluronic acid polymer having a modified structure.
34. The recombinant host cell of claim 29, wherein the enzymatically active hyaluronan synthase is capable of producing a hyaluronic acid polymer having a modified size distribution.
35. A purified composition, wherein the purified composition comprises an enzymatically active hyaluronan synthase polypeptide.
36. A purified composition, wherein the purified composition comprises a polypeptide having an amino acid sequence in accordance with SEQ ID NO:2.
37. A method for detecting a DNA species, comprising the steps of obtaining a DNA sample;
Contacting the DNA sample with a purified nucleic acid segment in accordance with SEQ ID NO:1;
hybridizing the DNA sample and the purified nucleic acid segment thereby forming a hybridized complex; and detecting the complex.
38. A method for detecting a bacterial cell that expresses mRNA encoding Streptococcus equisimilis hyaluronan synthase, comprising the steps of:
obtaining a bacterial cell sample;
contacting at least one nucleic acid from the bacterial cell sample with purified nucleic acid segment in accordance with SEQ ID NO:1;
hybridizing the at least one nucleic acid and the purified nucleic acid segment thereby forming a hybridized complex;
and detecting the hybridized complex, wherein the presence of the hybridized complex is indicative of a bacterial strain that expresses mRNA encoding Streptococcus equisimilis hyaluronan synthase.
39. A method for producing hyaluronic acid, comprising the steps of:
introducing a purified nucleic acid segment having a coding region encoding enzymatically active hyaluronan synthase into a host organism, wherein the host organism contains nucleic acid segments encoding enzymes Which produce UDP-GlcNAc and UDP-GlcA;
growing the host organism in a medium to secrete hyaluronic acid; and recovering the secreted hyaluronic acid.
40. The method according to claim 39, Wherein the step of recovering the hyaluronic acid comprises extracting the secreted hyaluronic acid from the medium.
41. The method according to claim 40, further comprising the step of purifying the extracted hyaluronic acid.
42. The method according to claim 39, wherein in the step of growing the host organism, the host organism secretes a structurally modified hyaluronic acid.
43. The method according to claim 39, wherein in the step of growing the host organism, the host organism secretes a hyaluronic acid having a modified size.
44. A pharmaceutical composition comprising a preselected pharmaceutical drug and an effective amount of hyaluronic acid produced by hyaluronan synthase.
45. The pharmaceutical composition of claim 44, wherein the hyaluronic acid is produced by the Streptococcus equisimilis hyaluronan synthase of SEQ ID NO:2.
46. The pharmaceutical composition according to claim 44, wherein the molecular weight of the hyaluronic acid is modified thereby producing a modified molecular weight pharmaceutical composition capable of evading an immune response.
47. The pharmaceutical composition according to claim 44, wherein the molecular weight of the hyaluronic acid is modified thereby producing a modified molecular weight pharmaceutical composition capable of targeting a specific tissue or cell type within the patient having an affinity for the modified molecular weight pharmaceutical composition.
48. A purified and isolated nucleic acid sequence encoding enzymatically active hyaluronan synthase, the nucleic acid sequence selected from the group consisting of:
(a) the nucleic acid sequence in accordance with SEQ ID NO:1;
(b) complementary nucleic acid sequences to the nucleic acid sequence in accordance with SEQ ID NO:1;

(c) nucleic acid sequences which will hybridize to the nucleic acid in accordance with SEQ ID NO:1;
(d) nucleic acid sequences which will hybridize to the complementary nucleic acid sequences of SEQ ID NO:1; and (e) nucleic acid sequences which will hybridize to PCR probes selected from the group consisting of PCR probes of SEQ
ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6.
49. A purified and isolated nucleic acid segment consisting essentially of a nucleic acid segment encoding enzymatically active hyaluronan synthase.
50. A procaryotic or eucaryotic host cell transformed or transfected with an isolated nucleic acid segment according to claim 1, 2, or 3 in a manner allowing the host cell to express hyaluronic acid.
51. An isolated nucleic acid segment consisting essentially of a nucleic acid segment encoding hyaluronan synthase having a nucleic acid segment sufficiently duplicative of the nucleic acid segment in accordance of SEQ ID NO:1 to allow possession of the biological property of encoding for Streptococcus equisimilis hyaluronan synthase.
52. A cDNA sequence according to claim 51.
53. A procaryotic or eucaryotic host cell transformed or transfected With a nucleic acid segment according to claim 51 in a manner allowing the host cell to express hyaluronic acid.
54. A purified nucleic acid segment having a coding region encoding enzymatically active hyaluronan synthase, wherein the purified nucleic acid segment is capable of hybridizing to the nucleotide sequence in accordance with SEQ ID NO:1.
55. A purified nucleic acid segment according to SEQ ID NO:3 capable of hybridizing to SEQ ID NO:1.
56. A purified nucleic acid segment according to SEQ ID NO:4 capable of hybridizing to SEQ ID NO:1.
57. A purified nucleic acid segment according to SEQ ID NO:5 capable of hybridizing to SEQ ID NO:1.
58. A purified nucleic acid segment according to SEQ ID NO:6 capable of hybridizing to SEQ ID NO:1.
59. A purified nucleic acid segment having a coding region encoding enzymatically active hyaluronate synthase, tie purified nucleic acid segment selected from the group consisting of:
(A) the nucleic acid segment according to SEQ ID NO: 2;

(B) the nucleotide sequence in accordance with SEQ ID
NO: 1;
(C) nucleic acid segments which hybridize to the nucleic acid segments defined in (A) or (B) or fragments thereof; and (D) nucleic acid segments which but for the degeneracy of the genetic code, or encoding of functionally equivalent amino acids, would hybridize to the nucleic acid segments defined in (A), (B), and (C).
CA2307842A 1997-10-31 1998-10-30 Hyaluronan synthase gene and uses thereof Expired - Lifetime CA2307842C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US6443597P 1997-10-31 1997-10-31
US60/064,435 1997-10-31
US17885198A 1998-10-26 1998-10-26
US09/178,851 1998-10-26
PCT/US1998/023153 WO1999023227A2 (en) 1997-10-31 1998-10-30 Hyaluronan synthase gene and uses thereof

Publications (2)

Publication Number Publication Date
CA2307842A1 true CA2307842A1 (en) 1999-05-14
CA2307842C CA2307842C (en) 2010-10-19

Family

ID=26744517

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2307842A Expired - Lifetime CA2307842C (en) 1997-10-31 1998-10-30 Hyaluronan synthase gene and uses thereof

Country Status (13)

Country Link
US (10) US6833264B1 (en)
EP (2) EP1522579A3 (en)
JP (1) JP4731013B2 (en)
KR (1) KR20010031682A (en)
CN (1) CN1322121C (en)
AT (1) ATE284954T1 (en)
AU (2) AU762036B2 (en)
BR (1) BR9814834B1 (en)
CA (1) CA2307842C (en)
DE (1) DE69828193T2 (en)
DK (1) DK1025211T3 (en)
ES (1) ES2235378T3 (en)
WO (1) WO1999023227A2 (en)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7091008B1 (en) 1994-07-01 2006-08-15 The Board Of Regents Of The University Of Oklahoma Hyaluronan synthase genes and expression thereof in Bacillus hosts
DE69828193T2 (en) * 1997-10-31 2005-12-01 The Board Of Regents Of The University Of Oklahoma, Norman HYALURONAN SYNTHASE GENE AND ITS USE
US7060469B2 (en) * 1998-04-02 2006-06-13 The Board Of Regents Of The University Of Oklahoma Polymer grafting by polysaccharide synthases
IL138803A0 (en) * 1998-04-02 2001-10-31 Univ Oklahoma Nucleic acid encoding hyaluronan synthase
US7534589B2 (en) * 1999-11-10 2009-05-19 The Board Of Regents Of The University Of Oklahoma Polymer grafting by polysaccharide synthases
US20070020737A1 (en) * 2001-12-03 2007-01-25 Pummill Philip E Hyaluronan synthases and methods of making and using same
HU228693B1 (en) * 2001-12-21 2013-05-28 Novozymes Biopharma Dk As Methods for producing hyaluronan in a recombinant host cell
AU2008200910C1 (en) * 2001-12-21 2012-11-22 Novozymes A/S Methods for producing hyaluronan in a recombinant host cell
US9733625B2 (en) * 2006-03-20 2017-08-15 General Electric Company Trip optimization system and method for a train
ES2280749T3 (en) * 2002-06-20 2007-09-16 Novozymes Biopolymer A/S FLOCULATION WITH BIVALENT SALT.
WO2005012529A1 (en) * 2003-07-31 2005-02-10 Toyo Boseki Kabushiki Kaisha Plant producing hyaluronic acid
EP1640457A1 (en) * 2004-09-23 2006-03-29 Bayer CropScience GmbH Process and means for the production of hyaluronan
PL1805312T3 (en) 2004-09-23 2009-12-31 Bayer Ip Gmbh Methods and means for producing hyaluronan
CN101123942A (en) * 2005-01-03 2008-02-13 诺维信生物聚合物公司 Hyaluronic acid fraction with moisturizing and anti-wrinkle properties
JP2006204260A (en) * 2005-01-31 2006-08-10 Toyobo Co Ltd Hyaluronic acid synthase and its gene
BRPI0614885A2 (en) * 2005-08-25 2011-04-19 Toyo Boseki hyaluronic acid producing plant
CN101297041A (en) * 2005-10-05 2008-10-29 拜尔作物科学股份公司 Plants with an increased production of hyaluronan II
PL1951878T3 (en) * 2005-10-05 2015-07-31 Bayer Ip Gmbh Plants with increased hyaluronan production
CA2624973C (en) * 2005-10-05 2016-01-19 Bayer Cropscience Ag Production of hyaluronan from plants transgenic for hyaluronan synthase, gfat and udp-glucose dehydrogenase
EP1772052A1 (en) * 2005-10-05 2007-04-11 Bayer CropScience GmbH Improved methods and means for production of hyaluronic acid
JP2007174957A (en) * 2005-12-27 2007-07-12 Toyobo Co Ltd Yeast for producing hyaluronic acid
AU2007280846B2 (en) 2006-08-04 2012-06-14 Novozymes Biopharma Dk A/S Branched hyaluronic acid and method of manufacture
CN101796189A (en) * 2007-03-29 2010-08-04 Cbd技术有限公司 Transgenic plants containing soluble cell wall polysaccharides
AU2008291695A1 (en) * 2007-08-31 2009-03-05 Sugar Industry Innovation Pty Ltd Production of hyaluronic acid
US9109051B2 (en) 2007-12-19 2015-08-18 Evonik Goldschmidt Gmbh Crosslinked hyaluronic acid in emulsion
JP2014515426A (en) 2011-05-30 2014-06-30 ノボザイムス バイオファーマ デーコー アクティーゼルスカブ Spray drying of high molecular weight hyaluronic acid
KR20140058581A (en) 2011-09-02 2014-05-14 노보자임스 바이오파마 디케이 에이/에스 Oral formulations containing hyaluronic acid for sustained drug release
WO2014100837A1 (en) 2012-12-17 2014-06-26 Bui The Duy Computer aided implantation of body implants
EP4269565A3 (en) * 2013-03-15 2024-01-24 Nutech Medical, Inc. Preparations derived from placental materials of making and using same
KR102135044B1 (en) * 2018-12-10 2020-07-17 대화제약 주식회사 Mutant protein of hyaluronic acid synthase and method of producing hyaluronic acid using the system
RU2719140C1 (en) 2019-07-26 2020-04-17 Общество с ограниченной ответственностью "ЦЕНТР ТРАНСФЕРА БИОТЕХНОЛОГИЙ ОКА-Биотех" Bacillus genus bacteria producing hyaluronic acid, and method of producing hyaluronic acid using said bacteria

Family Cites Families (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH624011A5 (en) 1977-08-05 1981-07-15 Battelle Memorial Institute
US4235871A (en) 1978-02-24 1980-11-25 Papahadjopoulos Demetrios P Method of encapsulating biologically active materials in lipid vesicles
ZA811368B (en) 1980-03-24 1982-04-28 Genentech Inc Bacterial polypedtide expression employing tryptophan promoter-operator
DE3160929D1 (en) 1980-04-17 1983-10-27 Ciba Geigy Ag Phenyl ureas
US4517295A (en) 1983-02-18 1985-05-14 Diagnostic, Inc. Hyaluronic acid from bacterial culture
CA1167797A (en) 1983-06-30 1984-05-22 Herbert E. Gladish Air conveyor components
JPS6060177A (en) 1983-09-12 1985-04-06 Hiroyuki Yugawa Freezing mixture for cooling
US4511478A (en) 1983-11-10 1985-04-16 Genetic Systems Corporation Polymerizable compounds and methods for preparing synthetic polymers that integrally contain polypeptides
US4615697A (en) 1983-11-14 1986-10-07 Bio-Mimetics, Inc. Bioadhesive compositions and methods of treatment therewith
US4983392A (en) 1983-11-14 1991-01-08 Bio-Mimetics, Inc. Bioadhesive compositions and methods of treatment therewith
NO161573C (en) 1983-11-25 1989-08-30 Miles Inc PROCEDURE FOR THE PREPARATION OF HYALURONIC ACID.
US4708861A (en) 1984-02-15 1987-11-24 The Liposome Company, Inc. Liposome-gel compositions
JPS60251898A (en) 1984-05-25 1985-12-12 Shiseido Co Ltd Preparation of hyaluronic acid by fermentation method
FR2569724B1 (en) 1984-09-04 1991-02-08 Chisso Corp PROCESS FOR THE PREPARATION OF HYALURONIC ACID
JPS6163294A (en) * 1984-09-05 1986-04-01 Chisso Corp Production of hyaluronic acid through fermentation process
US5171689A (en) 1984-11-08 1992-12-15 Matsushita Electric Industrial Co., Ltd. Solid state bio-sensor
US4784990A (en) * 1985-01-18 1988-11-15 Bio-Technology General Corporation High molecular weight sodium hyaluronate
US4780414A (en) 1985-01-18 1988-10-25 Bio-Technology General Corp. Method of producing high molecular weight sodium hyallronate by fermentation of streptococcus
IT1208514B (en) 1985-03-19 1989-07-10 Eniricerche Spa EXPRESSING PLASMID VECTORS IN ESCHERICHIA COLI AND / OR BACILLUSSUBTILIS.
JPS61267189A (en) 1985-05-22 1986-11-26 株式会社東芝 Receipts totalizer with bundler
JPS6232893A (en) 1985-08-01 1987-02-12 Kyowa Hakko Kogyo Co Ltd Production of hyaluronic acid
US5023175A (en) 1986-05-01 1991-06-11 Kabushiki Kaisha Yakult Honsha Novel production process of hyaluronic acid and bacterium strain therefor
JPS6394988A (en) 1986-10-08 1988-04-26 Meiji Seika Kaisha Ltd Production of hyaluronic acid
JPH072117B2 (en) 1986-10-09 1995-01-18 三菱レイヨン株式会社 Hyaluronic acid manufacturing method
EP0265578A1 (en) 1986-10-30 1988-05-04 Jan-Olof Eriksson A non-abrasive polish or cleaning composition and process for its preparation
US4822867A (en) 1987-02-02 1989-04-18 Semih Erhan Protein polymer grafts
JPH02193818A (en) 1989-01-23 1990-07-31 Okura Yusoki Co Ltd Pallet loading system
US5015577A (en) * 1989-08-29 1991-05-14 Board Of Regents, The University Of Texas System DNA encoding hyaluronate synthase
FR2652736A1 (en) 1989-10-06 1991-04-12 Neftel Frederic IMPLANTABLE DEVICE FOR EVALUATING THE RATE OF GLUCOSE.
JP2986519B2 (en) 1990-07-24 1999-12-06 生化学工業株式会社 Phospholipid-bound glycosaminoglycan
JPH04124854A (en) 1990-09-17 1992-04-24 Nikko Kyodo Co Ltd Manufacture of semiconductor device
CA2061703C (en) * 1992-02-20 2002-07-02 Rudolf E. Falk Formulations containing hyaluronic acid
JPH04134854A (en) 1990-09-26 1992-05-08 Shimadzu Corp Method for wiring between ic chips
JPH04158798A (en) 1990-10-19 1992-06-01 Tdk Corp Measurement of enzyme activity
JPH0630605B2 (en) 1990-10-23 1994-04-27 チッソ株式会社 Method for producing sodium hyaluronate aqueous solution
IT1250421B (en) 1991-05-30 1995-04-07 Recordati Chem Pharm CONTROLLED RELEASE PHARMACEUTICAL COMPOSITION WITH BIO-ADHESIVE PROPERTIES.
US5217743A (en) 1992-02-07 1993-06-08 Paradigm Biotechnologies Partnership Biomaterials of enhanced biocompatibility
US5621089A (en) 1992-05-27 1997-04-15 Novo Nordisk Biotech, Inc. Nucleic acid constructs for the production of a Bacillus alkaline protease
IT1260153B (en) * 1992-06-19 1996-03-28 Michael O'regan IDENTIFICATION OF A DNA FRAGMENT CODING THE HYALURONIC ACID SYNTHETASE (HAS) AND ITS NUCLEOTID SEQUENCE
WO1995022316A1 (en) 1994-02-17 1995-08-24 New York Blood Center, Inc. Biologic bioadhesive compositions containing fibrin glue and liposomes, methods of preparation and use
IT1268954B1 (en) 1994-03-11 1997-03-18 Fidia Advanced Biopolymers Srl PROCESS FOR THE PREPARATION OF HYALURONIC ACID BY MEANS OF ENZYMATIC SYNTHESIS AND RELATED PHARMACEUTICAL COMPOSITIONS
US5473034A (en) 1994-03-18 1995-12-05 Hyogo Prefectural Government Method for producing protein-synthetic polymer conjugate and said conjugate produced thereby
WO1995031548A1 (en) 1994-05-16 1995-11-23 The Uab Research Foundation Streptococcus pneumoniae capsular polysaccharide genes and flanking regions
SE9401806D0 (en) 1994-05-26 1994-05-26 Pharmacia Ab Method and means for the production of hyaluronic acid
US7091008B1 (en) * 1994-07-01 2006-08-15 The Board Of Regents Of The University Of Oklahoma Hyaluronan synthase genes and expression thereof in Bacillus hosts
US6455304B1 (en) * 1994-07-01 2002-09-24 The Board Of Regents Of The University Of Oklahoma Hyaluronate synthase gene and uses thereof
US6951743B2 (en) * 1997-10-31 2005-10-04 University Of Oklahoma Board Of Regents Hyaluronan synthase genes and expression thereof in bacillus hosts
JPH0838336A (en) 1994-08-01 1996-02-13 Matsushita Electric Ind Co Ltd Cover for electric carpet
CN1161060A (en) * 1994-09-12 1997-10-01 M·G·伯杰龙 Specific and universal probes and amplification primers to rapidly detect and identify common bacterial pathogens and antibiotic resistance genes from clinical specimens for routine diagnosis Lab.
US5831057A (en) * 1995-10-05 1998-11-03 Cold Spring Harbor Laboratory Associative learning and the linotte gene
NZ323834A (en) 1995-11-28 2000-01-28 Genvec Inc Chimeric adenovirus coat protein vectors and methods for gene transfer to cells
WO1997020061A1 (en) * 1995-11-30 1997-06-05 The Scripps Research Institute Synthesis of hyaluronic acid
SE9504304D0 (en) 1995-11-30 1995-11-30 Sandvik Ab Coated milling insert
US6423514B1 (en) 1996-04-22 2002-07-23 Millennium Pharmaceuticals, Inc. Mammalian hyaluronan synthases, nucleic acids and uses thereof
US5610241A (en) 1996-05-07 1997-03-11 Cornell Research Foundation, Inc. Reactive graft polymer with biodegradable polymer backbone and method for preparing reactive biodegradable polymers
US6602693B1 (en) * 1996-07-03 2003-08-05 Clear Solutions Biotech, Inc. Gene encoding hyaluronan synthase
US6492150B1 (en) 1996-07-03 2002-12-10 Clear Colutions Biotech, Inc. Gene encoding hyaluronan synthase
CN101113436B (en) * 1997-10-31 2013-02-06 俄克拉何马大学董事会 Hyaluronan synthase gene and uses thereof
DE69828193T2 (en) * 1997-10-31 2005-12-01 The Board Of Regents Of The University Of Oklahoma, Norman HYALURONAN SYNTHASE GENE AND ITS USE
US20080108110A1 (en) * 1998-04-02 2008-05-08 Deangelis Paul L Targeted glycosaminoglycan polymers by polymer grafting and methods of making and using same
US7223571B2 (en) * 1998-04-02 2007-05-29 The Board Of Regents Of The Universtiy Of Oklahoma Targeted glycosaminoglycan polymers by polymer grafting and methods of making and using same
US6987023B2 (en) * 1998-04-02 2006-01-17 The Board Of Regents Of The University Of Oklahoma DNA encoding hyaluronan synthase from Pasteurella multocida and methods of use
US20060188966A1 (en) 1998-04-02 2006-08-24 Deangelis Paul L Natural, chimeric and hybrid glycosaminoglycan polymers and methods of making and using same
US7060469B2 (en) * 1998-04-02 2006-06-13 The Board Of Regents Of The University Of Oklahoma Polymer grafting by polysaccharide synthases
US7094581B2 (en) 1998-10-26 2006-08-22 The Board Of Regents Of The University Of Oklahoma Hyaluronan synthases and methods of making and using same
US20060105431A1 (en) * 1998-11-11 2006-05-18 Deangelis Paul L Polymer grafting by polysaccharide synthases using artificial sugar acceptors
CA2351593C (en) * 1998-11-11 2012-02-21 The Board Of Regents Of The University Of Oklahoma Polymer grafting by polysaccharide synthases
US7534589B2 (en) * 1999-11-10 2009-05-19 The Board Of Regents Of The University Of Oklahoma Polymer grafting by polysaccharide synthases
HU228693B1 (en) * 2001-12-21 2013-05-28 Novozymes Biopharma Dk As Methods for producing hyaluronan in a recombinant host cell
EP1768678A4 (en) * 2004-06-30 2009-09-23 Paul L Deangelis Methods of selectively treating diseases with specific glycosaminoglycan polymers

Also Published As

Publication number Publication date
JP2001521741A (en) 2001-11-13
ATE284954T1 (en) 2005-01-15
US7087413B2 (en) 2006-08-08
US20060013829A1 (en) 2006-01-19
US20060216793A1 (en) 2006-09-28
US7504246B2 (en) 2009-03-17
US20030082780A1 (en) 2003-05-01
US7115405B2 (en) 2006-10-03
AU762036B2 (en) 2003-06-19
BR9814834B1 (en) 2011-08-23
US20050202540A1 (en) 2005-09-15
CA2307842C (en) 2010-10-19
AU1370699A (en) 1999-05-24
WO1999023227A9 (en) 2000-07-13
US20060018924A1 (en) 2006-01-26
JP4731013B2 (en) 2011-07-20
ES2235378T3 (en) 2005-07-01
US7060466B2 (en) 2006-06-13
DE69828193D1 (en) 2005-01-20
EP1522579A2 (en) 2005-04-13
EP1025211B1 (en) 2004-12-15
DK1025211T3 (en) 2005-04-04
US20030073221A1 (en) 2003-04-17
US20070087416A1 (en) 2007-04-19
US7153677B2 (en) 2006-12-26
CN1322121C (en) 2007-06-20
US6833264B1 (en) 2004-12-21
DE69828193T2 (en) 2005-12-01
US20060216808A1 (en) 2006-09-28
KR20010031682A (en) 2001-04-16
EP1522579A3 (en) 2007-11-07
EP1025211A2 (en) 2000-08-09
US6991921B2 (en) 2006-01-31
US7109011B2 (en) 2006-09-19
US7141409B2 (en) 2006-11-28
WO1999023227A3 (en) 1999-08-19
US20060216794A1 (en) 2006-09-28
AU2003248204A1 (en) 2003-10-30
CN1283224A (en) 2001-02-07
WO1999023227A2 (en) 1999-05-14
BR9814834A (en) 2001-07-17
US7166450B2 (en) 2007-01-23

Similar Documents

Publication Publication Date Title
CA2307842A1 (en) Hyaluronan synthase gene and uses thereof
RU2206611C2 (en) DNA FRAGMENT, PLASMID VECTOR, METHOD FOR PREPARING POLYPEPTIDE AND POLYPEPTIDE ELICITING IL-1β PROTEASE ACTIVITY
CA2326821A1 (en) Nucleic acid encoding hyaluronan synthase and methods of use
JPH11513562A (en) Method for producing recombinant plasmid
CN107858340B (en) High-catalytic-activity D-fructose-6-phosphate aldolase A mutant, recombinant expression vector, genetically engineered bacterium and application thereof
Kutish et al. Analysis of 76 kb of the chlorella virus PBCV-1 330-kb genome: map positions 182 to 258
KR20110106286A (en) Fusion collagenase to which affinity tag is attched,and method for producing same
CA2327266A1 (en) Pesticidal toxins and nucleotide sequences which encode these toxins
Lin et al. Cloning and expression of the γ-polyglutamic acid synthetase gene pgsBCA in Bacillus subtilis WB600
US5712147A (en) DNA encoding Bacillus licheniformis PWD-1 keratinase
JP2546979B2 (en) Recombinant D-hydantoinase, production method and use thereof
KR102546738B1 (en) Glutamate-cysteine ligase variant and method of producing glutathione using thereof
CA2384122A1 (en) Cyclic depsipeptide synthetase and gene thereof, and mass production system for cyclic depsipeptide
CN103255156B (en) Site mutant nattokinase gene high in activity and thermal stability, and application thereof
CA2434414A1 (en) Polypeptide having .alpha.-isomaltosylglucosaccharide-forming enzyme activity
KR930702528A (en) DNA sequence encoding proteolytic enzyme X isolated and purified from Streptomyces lividan
RU2000108487A (en) Genes
CN101497863A (en) Method for preparing N-terminated acetylated thymosin alpha 1 and special engineering bacteria therefor
WO2022159742A1 (en) Novel engineered and chimeric nucleases
CN112708571A (en) Recombinant yeast for producing chondroitin sulfate with controllable molecular weight by fermentation and application thereof
JPH01211490A (en) Human nerve growth factor gene segment
CN113416725A (en) Recombinant chondroitinase ABC-I protein and preparation method and application thereof
JPH06303971A (en) New protein having nitrile hydratase activity, gene coding the protein and production of amide from nitrile using transformant containing the gene
JPH09191887A (en) Depolymerase of biodegradable polymer and its production
JP4258577B2 (en) Process for producing poly-3-hydroxyalkanoic acid

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20181030