CA2310854A1 - Isolation and identification of novel polymerases - Google Patents

Isolation and identification of novel polymerases Download PDF

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Publication number
CA2310854A1
CA2310854A1 CA002310854A CA2310854A CA2310854A1 CA 2310854 A1 CA2310854 A1 CA 2310854A1 CA 002310854 A CA002310854 A CA 002310854A CA 2310854 A CA2310854 A CA 2310854A CA 2310854 A1 CA2310854 A1 CA 2310854A1
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CA
Canada
Prior art keywords
isolation
identification
novel polymerases
polymerases
novel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002310854A
Other languages
French (fr)
Other versions
CA2310854C (en
Inventor
Walter Callen
Eric J. Mathur
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Enzymes LLC
Original Assignee
Diversa Corporation
Walter Callen
Eric J. Mathur
Verenium Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diversa Corporation, Walter Callen, Eric J. Mathur, Verenium Corporation filed Critical Diversa Corporation
Publication of CA2310854A1 publication Critical patent/CA2310854A1/en
Application granted granted Critical
Publication of CA2310854C publication Critical patent/CA2310854C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Abstract

The invention provides purified thermostable enzymes derived from various extremophilic prokaryotic organisms. The enzymes have polymerase activity and can be used to catalyze DNA synthesis by addition of deoxynucleotides to the 3' end of a polynucleotide chain, using complementary polynucleotide strands as a template.
CA002310854A 1997-08-06 1998-08-06 Isolation and identification of novel polymerases Expired - Fee Related CA2310854C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/907,166 US5948666A (en) 1997-08-06 1997-08-06 Isolation and identification of polymerases
US08/907,166 1997-08-06
PCT/US1998/017152 WO1999007837A1 (en) 1997-08-06 1998-08-06 Isolation and identification of novel polymerases

Publications (2)

Publication Number Publication Date
CA2310854A1 true CA2310854A1 (en) 1999-02-18
CA2310854C CA2310854C (en) 2005-04-26

Family

ID=25423626

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002310854A Expired - Fee Related CA2310854C (en) 1997-08-06 1998-08-06 Isolation and identification of novel polymerases

Country Status (6)

Country Link
US (2) US5948666A (en)
EP (1) EP1002058A4 (en)
JP (1) JP2001512678A (en)
AU (1) AU735082B2 (en)
CA (1) CA2310854C (en)
WO (1) WO1999007837A1 (en)

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US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US7148054B2 (en) * 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
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US7056703B2 (en) * 1997-08-06 2006-06-06 Diversa Corporation Polypeptides having polymerase activity and methods of use thereof
US5948666A (en) * 1997-08-06 1999-09-07 Diversa Corporation Isolation and identification of polymerases
US7049101B1 (en) * 1997-08-06 2006-05-23 Diversa Corporation Enzymes having high temperature polymerase activity and methods of use thereof
US6541011B2 (en) 1998-02-11 2003-04-01 Maxygen, Inc. Antigen library immunization
US7390619B1 (en) * 1998-02-11 2008-06-24 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines
BR9910174A (en) 1998-05-01 2001-03-06 Maxygen Inc Process for obtaining an optimized recombinant gene for pest resistance, library, and process for obtaining an organism that is pathogenic to a vegetable pest
AU5347999A (en) 1998-08-12 2000-03-06 Maxygen, Inc. Dna shuffling of monooxygenase genes for production of industrial chemicals
WO2000009727A2 (en) * 1998-08-12 2000-02-24 Maxygen, Inc. Dna shuffling to produce herbicide selective crops
US20060242731A1 (en) * 1998-08-12 2006-10-26 Venkiteswaran Subramanian DNA shuffling to produce herbicide selective crops
US6500639B2 (en) 1998-10-07 2002-12-31 Maxygen, Inc. DNA shuffling to produce nucleic acids for mycotoxin detoxification
WO2000028018A1 (en) 1998-11-10 2000-05-18 Maxygen, Inc. Modified adp-glucose pyrophosphorylase for improvement and optimization of plant phenotypes
US6438561B1 (en) * 1998-11-19 2002-08-20 Navigation Technologies Corp. Method and system for using real-time traffic broadcasts with navigation systems
DE19859107A1 (en) * 1998-12-21 2000-06-29 Roche Diagnostics Gmbh Thermostable polymerase from ISS 1/1
US6917882B2 (en) * 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
EP1151409A1 (en) * 1999-01-18 2001-11-07 Maxygen, Inc. Methods of populating data stuctures for use in evolutionary simulations
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US20070065838A1 (en) * 1999-01-19 2007-03-22 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US7024312B1 (en) * 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
EP1108781A3 (en) 1999-01-19 2001-09-12 Maxygen, Inc. Method for making character strings, polynucleotides and polypeptides
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
KR20010102069A (en) 1999-02-11 2001-11-15 추후제출 High throughput mass spectrometry
JP3399518B2 (en) * 1999-03-03 2003-04-21 インターナショナル・ビジネス・マシーンズ・コーポレーション Semiconductor structure and method of manufacturing the same
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
EP1165775A2 (en) 1999-03-05 2002-01-02 Maxygen, Inc. Recombination of insertion modified nucleic acids
ATE278783T1 (en) * 1999-03-06 2004-10-15 Roche Diagnostics Gmbh DNA POLYMERASE FROM I(PYROBACULUM ISLANDICUM)
WO2001004287A1 (en) * 1999-07-07 2001-01-18 Maxygen Aps A method for preparing modified polypeptides
US20040002474A1 (en) * 1999-10-07 2004-01-01 Maxygen Inc. IFN-alpha homologues
US7430477B2 (en) * 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
US7115712B1 (en) 1999-12-02 2006-10-03 Maxygen, Inc. Cytokine polypeptides
AU2788101A (en) * 2000-01-11 2001-07-24 Maxygen, Inc. Integrated systems and methods for diversity generation and screening
WO2001064864A2 (en) * 2000-02-28 2001-09-07 Maxygen, Inc. Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation
WO2001073000A2 (en) * 2000-03-24 2001-10-04 Maxygen, Inc. Methods for modulating cellular and organismal phenotypes
US6677146B1 (en) * 2000-03-28 2004-01-13 Replidyne, Inc. Thermophilic polymerase III holoenzyme
EP1297146A2 (en) * 2000-06-23 2003-04-02 Maxygen, Inc. Novel chimeric promoters
WO2002000717A2 (en) * 2000-06-23 2002-01-03 Maxygen, Inc. Novel co-stimulatory molecules
WO2002004629A2 (en) * 2000-07-07 2002-01-17 Maxygen, Inc. Molecular breeding of transposable elements
US6858422B2 (en) * 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
US20020127623A1 (en) * 2000-07-31 2002-09-12 Maxygen, Inc. Biosensors, reagents and diagnostic applications of directed evolution
JP2004527215A (en) * 2000-08-24 2004-09-09 マキシジェン， インコーポレイテッド Constructs and their use in metabolic pathway engineering
US7745180B2 (en) * 2002-04-24 2010-06-29 Hitachi Chemical Co., Ltd. Device and method for high-throughput quantification of mRNA from whole blood
US20080311626A1 (en) * 2004-09-17 2008-12-18 Arkea Hf Dna Polymerases Having Strand Displacement Activity
US20060228726A1 (en) * 2005-01-06 2006-10-12 Martin Patrick K Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification
US20070059713A1 (en) * 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
CA2951416A1 (en) 2014-06-27 2015-12-30 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
SG11202012493WA (en) 2018-10-31 2021-01-28 Illumina Inc Polymerases, compositions, and methods of use
CN112639090A (en) 2018-12-05 2021-04-09 亿明达股份有限公司 Polymerases, compositions, and methods of use
CN113092425B (en) * 2021-03-29 2022-09-27 上海交通大学 Method for establishing programmable intercellular communication by DNA nano machine based on pH response

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US5491086A (en) * 1993-05-14 1996-02-13 Hoffmann-La Roche Inc. Purified thermostable nucleic acid polymerase and DNA coding sequences from pyrodictium species
USH1531H (en) * 1994-04-18 1996-05-07 Blumentals; Ilse I. Thermophilic DNA polymerase
JP3132624B2 (en) * 1994-05-09 2001-02-05 東洋紡績株式会社 DNA polymerase gene derived from hyperthermophilic archaeon and uses thereof
JP3498808B2 (en) * 1994-06-09 2004-02-23 タカラバイオ株式会社 DNA polymerase gene
US5948666A (en) * 1997-08-06 1999-09-07 Diversa Corporation Isolation and identification of polymerases

Also Published As

Publication number Publication date
EP1002058A1 (en) 2000-05-24
JP2001512678A (en) 2001-08-28
CA2310854C (en) 2005-04-26
WO1999007837A1 (en) 1999-02-18
US6492511B2 (en) 2002-12-10
AU8914398A (en) 1999-03-01
US20020013455A1 (en) 2002-01-31
EP1002058A4 (en) 2004-08-18
AU735082B2 (en) 2001-06-28
US5948666A (en) 1999-09-07

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