CA2316199A1 - Biosensor - Google Patents

Biosensor Download PDF

Info

Publication number
CA2316199A1
CA2316199A1 CA002316199A CA2316199A CA2316199A1 CA 2316199 A1 CA2316199 A1 CA 2316199A1 CA 002316199 A CA002316199 A CA 002316199A CA 2316199 A CA2316199 A CA 2316199A CA 2316199 A1 CA2316199 A1 CA 2316199A1
Authority
CA
Canada
Prior art keywords
glucose
biosensor
gbp
reporter group
test sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002316199A
Other languages
French (fr)
Other versions
CA2316199C (en
Inventor
Homme W. Hellinga
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Duke University
Original Assignee
Duke University
Homme W. Hellinga
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Duke University, Homme W. Hellinga filed Critical Duke University
Publication of CA2316199A1 publication Critical patent/CA2316199A1/en
Application granted granted Critical
Publication of CA2316199C publication Critical patent/CA2316199C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/66—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood sugars, e.g. galactose
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/808—Optical sensing apparatus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/817—Enzyme or microbe electrode
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/805—Optical property

Abstract

The present invention relates to a glucose biosensor comprising a genetically engineered Glucose Binding Protein (GBP). In a specific embodiment, the invention relates to a GBP engineered to include mutations that allow site specific introduction of environmentally sensitive reporter groups. The signal of these prosthetic groups changes linearly with the degree of glucose binding. Thus, the glucose sensor of the invention can be used, for example, for detection of glucose in blood or industrial fermentation processes.

Claims (21)

1. A glucose biosensor comprising a glucose binding protein (GBP) and a reporter group that transduces a detectable signal, wherein said reporter group is attached to said GBP so that a signal transduced by said reporter group when said GBP is bound to glucose differs from a signal transduced by said reporter group when said GBP is not bound to glucose.
2. The biosensor according to claim 1 wherein said reporter group is attached to said GBP at a site distant from a glucose binding site of said GBP.
3. The biosensor according to claim 1 wherein said reporter group is attached to a glucose binding site of said GBP.
4. The biosensor according to claim 1 wherein said reporter group is a fluorophore.
5. The biosensor according to claim 1 wherein said reporter group is a redox cofactor.
6. The biosensor according to claim 1 wherein said GBP has a binding constant for glucose in the range of 0.8 µM to 20 mM.
7. The biosensor according to claim 6 wherein said GBP has a binding constant for glucose in the range of 1 mM to 20 mM.
8. The biosensor according to claim 1 wherein said GBP is a mutant bacterial protein.
9. The biosensor according to claim 8 wherein said GBP has a binding constant for glucose in the range of 0.8 µM to 20 mM.
10. The biosensor according to claim 9 wherein said GBP has a binding constant for glucose in the range of 1 mM to 20 mM.
11. The biosensor according to claim 9 wherein said mutant protein is a mutant E. coli protein.
12. The biosensor according to claim 11 wherein said GBP differs from wild type E. coli GBP at positions 154 and 183.
13. The biosensor according to claim 12 wherein alanines are present at positions 154 and 183.
14. The biosensor according to claim 13 wherein said reporter group is a fluorophore.
15. The biosensor according to claim 13 wherein said reporter group is attached to a cysteine residue at position 255.
16. A method of detecting the presence of glucose in a test sample comprising contacting said biosensor according to claim 1 with said test sample under conditions such that said biosensor can bind to glucose present in said test sample and comparing the signal transduced by said reporter group when said biosensor is contacted with said test sample with the signal transduced by said reporter group when said biosensor is contacted with a glucose-free control sample, wherein a difference in the signal transduced by said reporter group when said biosensor is contacted with said test sample, as compared to when said biosensor is contacted with said control sample, indicates that test sample contains glucose.
17. The method according to claim 16 wherein said test sample is a physiological fluid.
18. The method according to claim 17 wherein said physiological fluid is blood, urine, interstitial fluid of saliva.
19. A method of determining the concentration of glucose in a test sample comprising contacting said biosensor according to claim 1 with said test sample under conditions such that said biosensor can bind to glucose present in said test sample, and comparing the signal transduced by said reporter group when said biosensor is contacted with said test sample with a standard hyperbolic glucose binding curve prepared by measuring the signal transduced by said reporter group when said biosensor is contacted with control samples containing known quantities of glucose, and thereby making said determination.
20. The method according to claim 20 wherein said test sample is a physiological fluid.
21. The method according to claim 20 wherein said physiological fluid is blood, urine, interstitial fluid of saliva.
CA2316199A 1997-12-31 1998-12-31 Biosensor Expired - Fee Related CA2316199C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US7029397P 1997-12-31 1997-12-31
US60/070,293 1997-12-31
PCT/US1998/027882 WO1999034212A1 (en) 1997-12-31 1998-12-31 Biosensor

Publications (2)

Publication Number Publication Date
CA2316199A1 true CA2316199A1 (en) 1999-07-08
CA2316199C CA2316199C (en) 2012-03-27

Family

ID=22094408

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2316199A Expired - Fee Related CA2316199C (en) 1997-12-31 1998-12-31 Biosensor

Country Status (10)

Country Link
US (2) US6277627B1 (en)
EP (1) EP1044374B1 (en)
JP (1) JP4472169B2 (en)
AT (1) ATE410683T1 (en)
AU (1) AU760743B2 (en)
CA (1) CA2316199C (en)
DE (1) DE69840107D1 (en)
DK (1) DK1044374T3 (en)
ES (1) ES2314996T3 (en)
WO (1) WO1999034212A1 (en)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6922576B2 (en) * 1998-06-19 2005-07-26 Becton, Dickinson And Company Micro optical sensor device
CA2336985C (en) * 1998-07-17 2011-01-04 University Of Maryland, Baltimore Method of glucose or galactose detection with glucose/galactose binding protein
US6535753B1 (en) * 1998-08-20 2003-03-18 Microsense International, Llc Micro-invasive method for painless detection of analytes in extra-cellular space
FR2805820B1 (en) * 2000-03-01 2009-09-11 Pasteur Institut BIOSENSORS, PROCESS FOR OBTAINING THEM AND THEIR APPLICATIONS
JP4647742B2 (en) * 2000-03-13 2011-03-09 三菱化学メディエンス株式会社 Analytical method and analytical reagent for ascorbic acid
JP2002250730A (en) * 2001-02-27 2002-09-06 Shinwa Kako Kk Molecule sensor and its producing method
US20100248268A1 (en) * 2001-03-27 2010-09-30 Woods Daniel F Methods to utilize invertebrate chemosensory proteins for industrial and commercial uses
EP1421371B1 (en) * 2001-08-28 2014-03-19 Duke University Biosensor
US6767733B1 (en) 2001-10-10 2004-07-27 Pritest, Inc. Portable biosensor apparatus with controlled flow
US7851593B2 (en) 2002-01-04 2010-12-14 Becton, Dickinson And Company Binding proteins as biosensors
US20050239155A1 (en) * 2002-01-04 2005-10-27 Javier Alarcon Entrapped binding protein as biosensors
US6855556B2 (en) 2002-01-04 2005-02-15 Becton, Dickinson And Company Binding protein as biosensors
US20030153026A1 (en) * 2002-01-04 2003-08-14 Javier Alarcon Entrapped binding protein as biosensors
US7064103B2 (en) 2002-01-04 2006-06-20 Becton, Dickinson And Company Binding protein as biosensors
US20030232370A1 (en) * 2002-04-22 2003-12-18 Trifiro Mark A. Glucose sensor and uses thereof
US20040060987A1 (en) * 2002-05-07 2004-04-01 Green Larry R. Digital image analysis method for enhanced and optimized signals in fluorophore detection
DK1552303T3 (en) * 2002-10-16 2009-05-25 Univ Duke Biosensor for detecting glucose
ATE412177T1 (en) * 2002-12-20 2008-11-15 Univ Wuerzburg J Maximilians MILLISECOND ACTIVATION SWITCH FOR 7- TRANSMEMBRANE PROTEINS
US20040234962A1 (en) * 2003-05-02 2004-11-25 Javier Alarcon Multicoated or multilayer entrapment matrix for protein biosensor
WO2005007806A2 (en) * 2003-05-07 2005-01-27 Duke University Protein design for receptor-ligand recognition and binding
WO2004101769A2 (en) * 2003-05-09 2004-11-25 University Of Maryland Baltimore County Proteins, sensors, and methods of characterizing analytes using the same
US20050112685A1 (en) * 2003-11-26 2005-05-26 Amiss Terry J. Compositions and methods for measuring analyte concentrations
US7787923B2 (en) * 2003-11-26 2010-08-31 Becton, Dickinson And Company Fiber optic device for sensing analytes and method of making same
US7496392B2 (en) * 2003-11-26 2009-02-24 Becton, Dickinson And Company Fiber optic device for sensing analytes
US7563891B2 (en) * 2004-05-21 2009-07-21 Becton, Dickinson & Company Long wavelength thiol-reactive fluorophores
CA2998199A1 (en) 2004-06-01 2005-12-15 Kwalata Trading Limited Methods for use with stem cells involving culturing on a surface with antibodies
ATE476659T1 (en) * 2004-06-09 2010-08-15 Becton Dickinson Co SENSOR FOR MULTIPLE ANALYTES
DE102004029941B3 (en) * 2004-06-21 2005-12-15 Infineon Technologies Ag System for evaluating a sensor signal
EP1786834B1 (en) * 2004-07-14 2016-02-17 Glusense Ltd. Implantable power sources and sensors
US7777016B2 (en) * 2004-10-14 2010-08-17 Carnegie Institution Of Washington Neurotransmitter sensors and methods of using the same
CA2584108A1 (en) * 2004-10-14 2006-04-27 Carnegie Institution Of Washington Development of sensitive fret sensors and methods of using the same
DE102005007723A1 (en) 2005-02-18 2006-08-24 Heinrich Meurer Airborne landmine detection method
DE102005007724A1 (en) 2005-02-18 2006-08-31 Heinrich Meurer Airborne hydrocarbon exploration process
WO2006096213A1 (en) * 2005-03-03 2006-09-14 Carnegie Institution Of Washington Polyamine sensors and methods of using the same
WO2006096214A1 (en) * 2005-03-04 2006-09-14 Carnegie Institution Of Washington Environmentally stable sensors and methods of using the same
US7687263B2 (en) * 2005-05-11 2010-03-30 Becton, Dickinson And Company In vitro tumor angiogenesis model
ATE481412T1 (en) * 2005-10-14 2010-10-15 Carnegie Inst Of Washington PHOSPHATE BIOSENSORS AND METHOD FOR USE THEREOF
WO2007046786A2 (en) * 2005-10-14 2007-04-26 Carnegie Institution Of Washington Sucrose biosensors and methods of using the same
US20080311047A1 (en) * 2005-11-16 2008-12-18 Thijs Kaper Multimetric Biosensors and Methods of Using Same
TW200734462A (en) * 2006-03-08 2007-09-16 In Motion Invest Ltd Regulating stem cells
JP5337691B2 (en) 2006-04-20 2013-11-06 ベクトン・ディキンソン・アンド・カンパニー Thermostable protein and method for producing and using the same
US7809441B2 (en) * 2006-05-17 2010-10-05 Cardiac Pacemakers, Inc. Implantable medical device with chemical sensor and related methods
US7708944B1 (en) * 2006-06-13 2010-05-04 Research Foundation Of State University Of New York Ultra-sensitive, portable capillary sensor
US20090093758A1 (en) 2006-07-24 2009-04-09 Yossi Gross Fibroid treatment apparatus and method
EP2150586B1 (en) * 2007-05-22 2018-03-14 Becton, Dickinson and Company Dyes having ratiometric fluorescence response for detecting metabolites
WO2009021039A1 (en) * 2007-08-06 2009-02-12 University Of Kentucky Research Foundation Device for detection of molecules of interest
US7673551B2 (en) 2007-08-15 2010-03-09 Heinrich Meurer Aerial-supported procedure for the detection of landmines
US7528949B2 (en) 2007-08-15 2009-05-05 Heinrich Meurer Aerial-supported procedure for exploration of hydrocarbon deposits
US9023661B2 (en) 2007-10-18 2015-05-05 Becton, Dickinson And Company Visual glucose sensor and methods of use thereof
US8541549B2 (en) * 2008-04-01 2013-09-24 University Of Southern California Annexin-based apoptosis markers
US20100160749A1 (en) * 2008-12-24 2010-06-24 Glusense Ltd. Implantable optical glucose sensing
EP3677293A1 (en) 2009-01-12 2020-07-08 Becton, Dickinson and Company In-dwelling rigid catheter with flexible features
US9375529B2 (en) 2009-09-02 2016-06-28 Becton, Dickinson And Company Extended use medical device
US8939928B2 (en) 2009-07-23 2015-01-27 Becton, Dickinson And Company Medical device having capacitive coupling communication and energy harvesting
US8403953B2 (en) * 2009-07-27 2013-03-26 Fibro Control, Inc. Balloon with rigid tube for occluding the uterine artery
US10092691B2 (en) 2009-09-02 2018-10-09 Becton, Dickinson And Company Flexible and conformal patch pump
US8741591B2 (en) 2009-10-09 2014-06-03 The Research Foundation For The State University Of New York pH-insensitive glucose indicator protein
US8814831B2 (en) 2010-11-30 2014-08-26 Becton, Dickinson And Company Ballistic microneedle infusion device
US9950109B2 (en) 2010-11-30 2018-04-24 Becton, Dickinson And Company Slide-activated angled inserter and cantilevered ballistic insertion for intradermal drug infusion
US8795230B2 (en) 2010-11-30 2014-08-05 Becton, Dickinson And Company Adjustable height needle infusion device
US9037205B2 (en) 2011-06-30 2015-05-19 Glusense, Ltd Implantable optical glucose sensing
US10466247B2 (en) 2012-11-20 2019-11-05 Becton, Dickinson And Company System and method for diagnosing sensor performance using analyte-independent ratiometric signals
US9456780B2 (en) * 2013-02-07 2016-10-04 Hill-Rom Services, Inc. Dynamic therapy delivery system
US10379125B2 (en) 2013-12-27 2019-08-13 Becton, Dickinson And Company System and method for dynamically calibrating and measuring analyte concentration in diabetes management monitors
US10004845B2 (en) 2014-04-18 2018-06-26 Becton, Dickinson And Company Split piston metering pump
US9416775B2 (en) 2014-07-02 2016-08-16 Becton, Dickinson And Company Internal cam metering pump
EP3206567A1 (en) 2014-10-13 2017-08-23 Glusense, Ltd. Analyte-sensing device
WO2016141363A2 (en) * 2015-03-05 2016-09-09 Duke University Glucose/galactose biosensors and methods of using same
US10716500B2 (en) 2015-06-29 2020-07-21 Cardiac Pacemakers, Inc. Systems and methods for normalization of chemical sensor data based on fluid state changes
WO2017087051A1 (en) * 2015-11-20 2017-05-26 Duke University Glucose biosensors and uses thereof
EP3377904B1 (en) 2015-11-20 2024-05-01 Duke University Urea biosensors and uses thereof
CA3005883A1 (en) * 2015-11-20 2017-05-26 Duke University Ratiometric biosensors and non-geometrically modulated fret
WO2017087916A2 (en) * 2015-11-20 2017-05-26 Duke University Thermostable glucose biosensors and uses thereof
EP3377899A4 (en) * 2015-11-20 2019-10-02 Duke University Bicarbonate biosensors, calcium biosensors, and uses thereof
US10871487B2 (en) 2016-04-20 2020-12-22 Glusense Ltd. FRET-based glucose-detection molecules
CN108968976B (en) 2017-05-31 2022-09-13 心脏起搏器股份公司 Implantable medical device with chemical sensor
CN109381195B (en) 2017-08-10 2023-01-10 心脏起搏器股份公司 Systems and methods including electrolyte sensor fusion
CN109419515B (en) 2017-08-23 2023-03-24 心脏起搏器股份公司 Implantable chemical sensor with staged activation
CN109864746B (en) 2017-12-01 2023-09-29 心脏起搏器股份公司 Multimode analyte sensor for medical devices
CN109864747B (en) 2017-12-05 2023-08-25 心脏起搏器股份公司 Multimode analyte sensor optoelectronic interface
EP4100956A4 (en) * 2020-02-03 2024-03-06 Her Majesty The Queen In Right Of Canada As Represented By The Mini Of Agriculture And Agri Food Improved processes for rationally-designing and producing biomolecules

Also Published As

Publication number Publication date
AU760743B2 (en) 2003-05-22
EP1044374B1 (en) 2008-10-08
DE69840107D1 (en) 2008-11-20
ES2314996T3 (en) 2009-03-16
CA2316199C (en) 2012-03-27
JP4472169B2 (en) 2010-06-02
DK1044374T3 (en) 2009-02-16
EP1044374A1 (en) 2000-10-18
US6521446B2 (en) 2003-02-18
US20020004217A1 (en) 2002-01-10
JP2002500361A (en) 2002-01-08
EP1044374A4 (en) 2002-11-27
US6277627B1 (en) 2001-08-21
WO1999034212A1 (en) 1999-07-08
AU2210199A (en) 1999-07-19
ATE410683T1 (en) 2008-10-15

Similar Documents

Publication Publication Date Title
CA2316199A1 (en) Biosensor
DE69809391D1 (en) SMALL VOLUME SENSOR FOR IN-VITRO DETERMINATION
Boubriak et al. Determination of urea in blood serum by a urease biosensor based on an ion-sensitive field-effect transistor
CA2471863A1 (en) Binding proteins as biosensors
CN1284652A (en) Electrochemical analysis for determinating initial time of sample detection
CA2566040A1 (en) Method for increasing the dynamic measuring range of test elements, especially immunological test elements, that are based on specific binding reactions
Toffaletti et al. Lactate measured in diluted and undiluted whole blood and plasma: comparison of methods and effect of hematocrit
EP0922954A3 (en) Method for recognizing the presence of sample fluid on a test strip by evaluating first and second derivatives
Harborn et al. Evaluation of a miniaturized thermal biosensor for the determination of glucose in whole blood
ATE166157T1 (en) DETECTION OF SYNDEC CONTENT IN BIOLOGICAL MATERIALS, SUCH AS TISSUES AND BODY FLUID, TO INDICATE MAGNIFICENT TRANSFORMATION OF CELLS
CN109690304A (en) The method that electrochemical analysis is carried out by using the alternating output signal from two electrodes
Barbaro et al. CHEMFET devices for biomedical and environmental applications
Cartier et al. Toxic levels of acetaminophen produce a major positive interference on Glucometer Elite and Accu-chek Advantage glucose meters
US20090148872A1 (en) Lipoprotein sensor
Eppelsheim et al. Potentiometric thick-film sensor for the determination of the neurotransmitter acetylcholine
CN111172234B (en) Glycylproline dipeptide aminopeptidase assay kit
Magner Detection of ferricyanide as a probe for the effect of hematocrit in whole blood biosensors
Campanella et al. Analysis Ofl-dopa in pharmaceutical preparations and of total phenols content in urine by means of an enzyme—amperometric sensor
Thornton et al. Fermentation glucose assay using the Exactech blood glucose biosensor
WO2004027083A2 (en) An analyzer for the simultaneous enzymatic detection of closely related analytes
Buckley et al. New ways with old ions
EP0161299A1 (en) Sensor for chemical analysis
EP0423235A1 (en) Improved biosensor and the method of its use
Bruns et al. Creatine kinase-MB activity: clinical and laboratory studies of specific immunochemical technique with optimized enzymatic assay
Lawson et al. Fructosamine assay

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20181231