CA2319705A1 - Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis - Google Patents
Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis Download PDFInfo
- Publication number
- CA2319705A1 CA2319705A1 CA002319705A CA2319705A CA2319705A1 CA 2319705 A1 CA2319705 A1 CA 2319705A1 CA 002319705 A CA002319705 A CA 002319705A CA 2319705 A CA2319705 A CA 2319705A CA 2319705 A1 CA2319705 A1 CA 2319705A1
- Authority
- CA
- Canada
- Prior art keywords
- chip
- cell
- cartridge
- dielectrophoresis
- separation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000926 separation method Methods 0.000 title abstract 3
- 238000004720 dielectrophoresis Methods 0.000 title abstract 2
- 239000000203 mixture Substances 0.000 abstract 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 abstract 1
- 230000021164 cell adhesion Effects 0.000 abstract 1
- 239000013592 cell lysate Substances 0.000 abstract 1
- 230000009089 cytolysis Effects 0.000 abstract 1
- 238000006911 enzymatic reaction Methods 0.000 abstract 1
- 239000006166 lysate Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000002245 particle Substances 0.000 abstract 1
- 230000001681 protective effect Effects 0.000 abstract 1
- 229910052710 silicon Inorganic materials 0.000 abstract 1
- 239000010703 silicon Substances 0.000 abstract 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
- B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C5/00—Separating dispersed particles from liquids by electrostatic effect
- B03C5/02—Separators
- B03C5/022—Non-uniform field separators
- B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C5/00—Separating dispersed particles from liquids by electrostatic effect
- B03C5/02—Separators
- B03C5/022—Non-uniform field separators
- B03C5/028—Non-uniform field separators using travelling electric fields, i.e. travelling wave dielectrophoresis [TWD]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44743—Introducing samples
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01L—SEMICONDUCTOR DEVICES NOT COVERED BY CLASS H10
- H01L29/00—Semiconductor devices adapted for rectifying, amplifying, oscillating or switching, or capacitors or resistors with at least one potential-jump barrier or surface barrier, e.g. PN junction depletion layer or carrier concentration layer; Details of semiconductor bodies or of electrodes thereof ; Multistep manufacturing processes therefor
- H01L29/66—Types of semiconductor device ; Multistep manufacturing processes therefor
- H01L29/66007—Multistep manufacturing processes
- H01L29/66075—Multistep manufacturing processes of devices having semiconductor bodies comprising group 14 or group 13/15 materials
- H01L29/66227—Multistep manufacturing processes of devices having semiconductor bodies comprising group 14 or group 13/15 materials the devices being controllable only by the electric current supplied or the electric potential applied, to an electrode which does not carry the current to be rectified, amplified or switched, e.g. three-terminal devices
- H01L29/66409—Unipolar field-effect transistors
- H01L29/66477—Unipolar field-effect transistors with an insulated gate, i.e. MISFET
- H01L29/6656—Unipolar field-effect transistors with an insulated gate, i.e. MISFET using multiple spacer layers, e.g. multiple sidewall spacers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/00851—Additional features
- B01J2219/00853—Employing electrode arrangements
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/00905—Separation
- B01J2219/00912—Separation by electrophoresis
- B01J2219/00914—Separation by electrophoresis by dielectrophoresis
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0631—Purification arrangements, e.g. solid phase extraction [SPE]
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0668—Trapping microscopic beads
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0636—Integrated biosensor, microarrays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0645—Electrodes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0877—Flow chambers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
- B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
- B01L2400/0424—Dielectrophoretic forces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0493—Specific techniques used
- B01L2400/0496—Travelling waves, e.g. in combination with electrical or acoustic forces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0633—Valves, specific forms thereof with moving parts
- B01L2400/0644—Valves, specific forms thereof with moving parts rotary valves
Abstract
The present invention comprises devices and methods for performing channel-less separation of cell particles by dielectrophoresis, DC high voltage-pulsed electronic lysis of separated cells, separation of desired components from crude mixtures such as cell lysates, and/or enzymatic reaction of such lysates, all of which can be conducted on a single bioelectronic chip. A preferred embodiment of the present invention comprises a cartridge (10) including a microfabricated silicon chip (12) on a printed circuit board (14) and a flow cell (16) mounted to the chip (12) to form a flow chamber. The cartridge (10) also includes output pins (22) for electronically connecting the cartridge (10) to an electronic controller. The chip (12) includes a plurality of circular microelectrodes (24) which are preferably coated with a protective permeation layer which prevents direct contact between any electrode and a sample introduced into the flow chamber. The permeation layer also helps to reduce cell adhesion at field minima, and enables immobilization of specific antibodies for specific cell capture. Specific cells from various cell mixtures were separated, lysed, and enzymatically digested on the chip.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/016,596 | 1998-01-30 | ||
US09/016,596 US6071394A (en) | 1996-09-06 | 1998-01-30 | Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis |
PCT/US1999/001651 WO1999038612A1 (en) | 1998-01-30 | 1999-01-26 | Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2319705A1 true CA2319705A1 (en) | 1999-08-05 |
CA2319705C CA2319705C (en) | 2008-07-15 |
Family
ID=21777962
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002319705A Expired - Fee Related CA2319705C (en) | 1998-01-30 | 1999-01-26 | Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis |
Country Status (12)
Country | Link |
---|---|
US (3) | US6071394A (en) |
EP (1) | EP1053055B1 (en) |
JP (1) | JP2002502047A (en) |
KR (1) | KR100512518B1 (en) |
CN (1) | CN1170942C (en) |
AT (1) | ATE246040T1 (en) |
AU (1) | AU743074B2 (en) |
BR (1) | BR9908349A (en) |
CA (1) | CA2319705C (en) |
DE (1) | DE69909971D1 (en) |
NZ (1) | NZ505858A (en) |
WO (1) | WO1999038612A1 (en) |
Families Citing this family (214)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6197508B1 (en) | 1990-09-12 | 2001-03-06 | Affymetrix, Inc. | Electrochemical denaturation and annealing of nucleic acid |
US7314708B1 (en) * | 1998-08-04 | 2008-01-01 | Nanogen, Inc. | Method and apparatus for electronic synthesis of molecular structures |
US6129828A (en) | 1996-09-06 | 2000-10-10 | Nanogen, Inc. | Apparatus and methods for active biological sample preparation |
US7582421B2 (en) * | 1993-11-01 | 2009-09-01 | Nanogen, Inc. | Methods for determination of single nucleic acid polymorphisms using a bioelectronic microchip |
US7857957B2 (en) * | 1994-07-07 | 2010-12-28 | Gamida For Life B.V. | Integrated portable biological detection system |
US6403367B1 (en) * | 1994-07-07 | 2002-06-11 | Nanogen, Inc. | Integrated portable biological detection system |
US6379897B1 (en) * | 2000-11-09 | 2002-04-30 | Nanogen, Inc. | Methods for gene expression monitoring on electronic microarrays |
US6071394A (en) * | 1996-09-06 | 2000-06-06 | Nanogen, Inc. | Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis |
US6355436B1 (en) * | 1996-05-17 | 2002-03-12 | L'ecole Centrale De Lyon | Method for analyzing biological substances in a conductive liquid medium |
US6287831B1 (en) * | 1997-11-14 | 2001-09-11 | California Institute Of Technology | Cell lysis device |
US20020144905A1 (en) * | 1997-12-17 | 2002-10-10 | Christian Schmidt | Sample positioning and analysis system |
ATE205300T1 (en) * | 1997-12-17 | 2001-09-15 | Ecole Polytech | POSITIONING AND ELECTROPHYSIOLOGICAL CHARACTERIZATION OF INDIVIDUAL CELLS AND RECONSTITUTED MEMBRANE SYSTEMS ON MICROSTRUCTURED SUPPORTS |
US20050053962A1 (en) * | 1998-01-27 | 2005-03-10 | Gary Blackburn | Amplification of nucleic acids with electronic detection |
US6780591B2 (en) | 1998-05-01 | 2004-08-24 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US7875440B2 (en) | 1998-05-01 | 2011-01-25 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US6093370A (en) * | 1998-06-11 | 2000-07-25 | Hitachi, Ltd. | Polynucleotide separation method and apparatus therefor |
US6387632B2 (en) * | 1998-06-11 | 2002-05-14 | Hitachi, Ltd. | Polynucleotide separation method and apparatus therefor |
GB9812783D0 (en) | 1998-06-12 | 1998-08-12 | Cenes Ltd | High throuoghput screen |
US20020012916A1 (en) * | 1998-10-14 | 2002-01-31 | Gundling Gerard J | A method of reducing contamination in an essay vessel |
AU763514B2 (en) * | 1998-12-23 | 2003-07-24 | Nanogen, Inc. | Integrated portable biological detection system |
US6294063B1 (en) * | 1999-02-12 | 2001-09-25 | Board Of Regents, The University Of Texas System | Method and apparatus for programmable fluidic processing |
CN1181337C (en) | 2000-08-08 | 2004-12-22 | 清华大学 | Solid molecule operating method in microfluid system |
US6858439B1 (en) * | 1999-03-15 | 2005-02-22 | Aviva Biosciences | Compositions and methods for separation of moieties on chips |
WO2000066785A2 (en) * | 1999-05-03 | 2000-11-09 | Gen-Probe Incorporated | Polynucleotide probes for detection and quantitation of bacteria in the family enterobacteriaceae |
US6821770B1 (en) | 1999-05-03 | 2004-11-23 | Gen-Probe Incorporated | Polynucleotide matrix-based method of identifying microorganisms |
US6811668B1 (en) | 1999-06-22 | 2004-11-02 | Caliper Life Sciences, Inc. | Apparatus for the operation of a microfluidic device |
EP1360992A3 (en) * | 1999-06-22 | 2004-05-19 | Caliper Life Sciences, Inc. | Apparatus for the operation of a microfluidic device |
US6818395B1 (en) * | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
US20040185462A1 (en) * | 1999-08-06 | 2004-09-23 | Tum Gene, Inc. | Method of and detecting apparatus and detecting chip for single base substitution SNP and point mutation of genes |
US6696022B1 (en) * | 1999-08-13 | 2004-02-24 | U.S. Genomics, Inc. | Methods and apparatuses for stretching polymers |
US6495104B1 (en) * | 1999-08-19 | 2002-12-17 | Caliper Technologies Corp. | Indicator components for microfluidic systems |
EP1080785A1 (en) * | 1999-09-04 | 2001-03-07 | F. Hoffmann-La Roche Ag | System for thermocycling of fluids in cartridges |
US20020132371A1 (en) * | 1999-09-27 | 2002-09-19 | Kreimer David I. | Amplification of analyte detection by substrates having particle structures with receptors |
US20030232388A1 (en) * | 1999-09-27 | 2003-12-18 | Kreimer David I. | Beads having identifiable Raman markers |
US20040023293A1 (en) * | 1999-09-27 | 2004-02-05 | Kreimer David I. | Biochips for characterizing biological processes |
KR20010099859A (en) | 1999-10-20 | 2001-11-09 | 시게오리 다케나카 | Gene detecting chip, detector, and detecting method |
US6824664B1 (en) | 1999-11-04 | 2004-11-30 | Princeton University | Electrode-less dielectrophorises for polarizable particles |
AU2427301A (en) * | 1999-12-01 | 2001-06-12 | Regents Of The University Of California, The | Electric-field-assisted fluidic assembly of inorganic and organic materials, molecules and like small things including living cells |
CA2364381C (en) * | 1999-12-22 | 2009-03-10 | Gene Logic, Inc. | Flow-thru chip cartridge, chip holder, system and method thereof |
US6824669B1 (en) * | 2000-02-17 | 2004-11-30 | Motorola, Inc. | Protein and peptide sensors using electrical detection methods |
US6537433B1 (en) * | 2000-03-10 | 2003-03-25 | Applera Corporation | Methods and apparatus for the location and concentration of polar analytes using an alternating electric field |
US6602400B1 (en) | 2000-06-15 | 2003-08-05 | Motorola, Inc. | Method for enhanced bio-conjugation events |
ATE322986T1 (en) * | 2000-06-15 | 2006-04-15 | Seiko Epson Corp | METHOD FOR FEEDING LIQUID, LIQUID CONTAINER AND METHOD FOR THE PRODUCTION THEREOF |
US6632400B1 (en) * | 2000-06-22 | 2003-10-14 | Agilent Technologies, Inc. | Integrated microfluidic and electronic components |
AU785424B2 (en) * | 2000-07-26 | 2007-05-17 | Regents Of The University Of California, The | Manipulation of live cells and inorganic objects with optical micro beam arrays |
US7270730B2 (en) * | 2000-08-04 | 2007-09-18 | Essen Instruments, Inc. | High-throughput electrophysiological measurement system |
EP1309863A1 (en) * | 2000-08-08 | 2003-05-14 | Aviva Biosciences Corporation | Methods for manipulating moieties in microfluidic systems |
CN100495030C (en) | 2000-09-30 | 2009-06-03 | 清华大学 | Multi-force operator and use thereof |
AU2001297014A1 (en) * | 2000-10-10 | 2002-04-22 | Aviva Biosciences Corporation | An integrated biochip system for sample preparation and analysis |
US6784420B2 (en) * | 2000-11-13 | 2004-08-31 | Genoptix, Inc. | Method of separating particles using an optical gradient |
US20030007894A1 (en) * | 2001-04-27 | 2003-01-09 | Genoptix | Methods and apparatus for use of optical forces for identification, characterization and/or sorting of particles |
US6833542B2 (en) * | 2000-11-13 | 2004-12-21 | Genoptix, Inc. | Method for sorting particles |
US20020160470A1 (en) * | 2000-11-13 | 2002-10-31 | Genoptix | Methods and apparatus for generating and utilizing linear moving optical gradients |
US6936811B2 (en) * | 2000-11-13 | 2005-08-30 | Genoptix, Inc. | Method for separating micro-particles |
US20020123112A1 (en) * | 2000-11-13 | 2002-09-05 | Genoptix | Methods for increasing detection sensitivity in optical dielectric sorting systems |
US6744038B2 (en) | 2000-11-13 | 2004-06-01 | Genoptix, Inc. | Methods of separating particles using an optical gradient |
US6778724B2 (en) * | 2000-11-28 | 2004-08-17 | The Regents Of The University Of California | Optical switching and sorting of biological samples and microparticles transported in a micro-fluidic device, including integrated bio-chip devices |
AU2002230979A1 (en) * | 2000-12-18 | 2002-07-01 | Trustees Of Princeton University | Fractionation of macro-molecules using asymmetric pulsed field electrophoresis |
DE60030306T2 (en) | 2000-12-28 | 2007-08-30 | Roche Diagnostics Gmbh | Method, system and cartridge for processing a nucleic acid sample by oscillation of the cartridge |
US6707548B2 (en) | 2001-02-08 | 2004-03-16 | Array Bioscience Corporation | Systems and methods for filter based spectrographic analysis |
US6626417B2 (en) | 2001-02-23 | 2003-09-30 | Becton, Dickinson And Company | Microfluidic valve and microactuator for a microvalve |
US7297518B2 (en) * | 2001-03-12 | 2007-11-20 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension |
US20020151041A1 (en) * | 2001-03-15 | 2002-10-17 | Kreimer David I. | Enhancing surfaces for analyte detection |
US7014742B2 (en) * | 2001-03-15 | 2006-03-21 | The Regents Of The University Of California | Positioning of organic and inorganic objects by electrophoretic forces, including for microlens alignment |
CN100494360C (en) * | 2001-03-22 | 2009-06-03 | 博奥生物有限公司 | Process for separating cells and its application |
CA2441366A1 (en) * | 2001-03-24 | 2002-10-03 | Aviva Biosciences Corporation | Biochips including ion transport detecting structures and methods of use |
US20030153024A1 (en) * | 2001-04-19 | 2003-08-14 | Sullivan Brian M | Charged bio-molecule/binding agent conjugate for biological capture |
US20030194755A1 (en) * | 2001-04-27 | 2003-10-16 | Genoptix, Inc. | Early detection of apoptotic events and apoptosis using optophoretic analysis |
US20040009540A1 (en) * | 2001-04-27 | 2004-01-15 | Genoptix, Inc | Detection and evaluation of cancer cells using optophoretic analysis |
ATE367205T1 (en) * | 2001-05-02 | 2007-08-15 | Applera Corp | CONCENTRATION AND PURIFICATION OF ANALYTES USING ELECTRICAL FIELDS |
ITTO20010411A1 (en) * | 2001-05-02 | 2002-11-02 | Silicon Biosystems S R L | METHOD AND DEVICE FOR THE EXECUTION OF TESTS AND TESTS WITH HIGH PROCESSIVITY AND HIGH BIOLOGICAL VALUE ON CELLS AND / OR COMPOUNDS. |
DE10122659A1 (en) * | 2001-05-10 | 2002-12-05 | Infineon Technologies Ag | Biochip arrangement |
US7014747B2 (en) * | 2001-06-20 | 2006-03-21 | Sandia Corporation | Dielectrophoretic systems without embedded electrodes |
US7419574B2 (en) * | 2001-06-20 | 2008-09-02 | Cummings Eric B | Dielectrophoresis device and method having non-uniform arrays for manipulating particles |
WO2003012030A2 (en) * | 2001-08-01 | 2003-02-13 | University Of Utah, Technology Transfer Office | Isoform-selective inhibitors and activators of pde3 cyclic |
US6890409B2 (en) * | 2001-08-24 | 2005-05-10 | Applera Corporation | Bubble-free and pressure-generating electrodes for electrophoretic and electroosmotic devices |
US8980568B2 (en) * | 2001-10-11 | 2015-03-17 | Aviva Biosciences Corporation | Methods and compositions for detecting non-hematopoietic cells from a blood sample |
US8986944B2 (en) * | 2001-10-11 | 2015-03-24 | Aviva Biosciences Corporation | Methods and compositions for separating rare cells from fluid samples |
US7597791B2 (en) | 2001-10-19 | 2009-10-06 | The Trustees Of Princeton University | Method and apparatus for generating electric fields and flow distributions for rapidly separating molecules |
US6783647B2 (en) * | 2001-10-19 | 2004-08-31 | Ut-Battelle, Llc | Microfluidic systems and methods of transport and lysis of cells and analysis of cell lysate |
FR2831084B1 (en) * | 2001-10-22 | 2004-08-27 | Centre Nat Rech Scient | METHOD AND SYSTEM FOR DIELECTROPHORESIS HANDLING OF DIELECTRIC PARTICLES, PARTICULARLY BIOLOGICAL CELLS |
US20030175947A1 (en) * | 2001-11-05 | 2003-09-18 | Liu Robin Hui | Enhanced mixing in microfluidic devices |
US6866762B2 (en) * | 2001-12-20 | 2005-03-15 | Board Of Regents, University Of Texas System | Dielectric gate and methods for fluid injection and control |
WO2003060505A1 (en) * | 2002-01-04 | 2003-07-24 | Board Of Regents, The University Of Texas System | Wall-less channels for fluidic routing and confinement |
DE10204652B4 (en) * | 2002-02-05 | 2004-07-22 | Infineon Technologies Ag | Circuit arrangement, electrochemical sensor, sensor arrangement and method for processing a current signal provided via a sensor electrode |
DE10321490B3 (en) * | 2002-02-05 | 2004-10-14 | Infineon Technologies Ag | Electrochemical sensor, as a biochip working on a redox cycling principle, has one circuit is a potentiostat coupled to the sensor electrode and a second circuit has a condenser for potential comparison |
US6887362B2 (en) * | 2002-02-06 | 2005-05-03 | Nanogen, Inc. | Dielectrophoretic separation and immunoassay methods on active electronic matrix devices |
US20030148362A1 (en) * | 2002-02-07 | 2003-08-07 | Eastern Virginia Medical School Of The Medical College Of Hampton Roads | Diagnostic microarray and method of use thereof |
GB0205455D0 (en) | 2002-03-07 | 2002-04-24 | Molecular Sensing Plc | Nucleic acid probes, their synthesis and use |
US20050100938A1 (en) * | 2002-03-14 | 2005-05-12 | Infineon Technologies Ag | Vertical impedance sensor arrangement and method for producing a vertical impedance sensor arrangement |
US20030211461A1 (en) * | 2002-05-01 | 2003-11-13 | Genoptix, Inc | Optophoretic detection of durgs exhibiting inhibitory effect on Bcr-Abl positive tumor cells |
US20040033539A1 (en) * | 2002-05-01 | 2004-02-19 | Genoptix, Inc | Method of using optical interrogation to determine a biological property of a cell or population of cells |
US20040011650A1 (en) * | 2002-07-22 | 2004-01-22 | Frederic Zenhausern | Method and apparatus for manipulating polarizable analytes via dielectrophoresis |
US7601493B2 (en) * | 2002-07-26 | 2009-10-13 | Nanogen, Inc. | Methods and apparatus for screening and detecting multiple genetic mutations |
GB0218009D0 (en) * | 2002-08-02 | 2002-09-11 | Imp College Innovations Ltd | DNA extraction microchip, system and method |
US20040053209A1 (en) * | 2002-09-12 | 2004-03-18 | Genoptix, Inc | Detection and evaluation of topoisomerase inhibitors using optophoretic analysis |
AU2003267175A1 (en) * | 2002-09-13 | 2004-04-30 | Valentis, Inc. | Apparatus and method for preparative scale purification of nucleic acids |
US7960184B2 (en) * | 2002-09-20 | 2011-06-14 | George Mason Intellectual Properties, Inc. | Methods and devices for active bioassay |
US7329545B2 (en) | 2002-09-24 | 2008-02-12 | Duke University | Methods for sampling a liquid flow |
US6911132B2 (en) | 2002-09-24 | 2005-06-28 | Duke University | Apparatus for manipulating droplets by electrowetting-based techniques |
ES2375724T3 (en) | 2002-09-27 | 2012-03-05 | The General Hospital Corporation | MICROFLUDE DEVICE FOR SEPERATION OF CELLS AND ITS USES. |
US20040067167A1 (en) * | 2002-10-08 | 2004-04-08 | Genoptix, Inc. | Methods and apparatus for optophoretic diagnosis of cells and particles |
CN1429917A (en) * | 2002-10-11 | 2003-07-16 | 薛永新 | AIDS virus in-situ nucleic acid chip and its preparation method |
US7932098B2 (en) * | 2002-10-31 | 2011-04-26 | Hewlett-Packard Development Company, L.P. | Microfluidic system utilizing thin-film layers to route fluid |
US20040086872A1 (en) * | 2002-10-31 | 2004-05-06 | Childers Winthrop D. | Microfluidic system for analysis of nucleic acids |
US20040121307A1 (en) * | 2002-12-19 | 2004-06-24 | Genoptix, Inc | Early detection of cellular differentiation using optophoresis |
US20040121474A1 (en) * | 2002-12-19 | 2004-06-24 | Genoptix, Inc | Detection and evaluation of chemically-mediated and ligand-mediated t-cell activation using optophoretic analysis |
WO2004098741A2 (en) * | 2003-04-30 | 2004-11-18 | Biovitesse | Apparatus and method for detecting live cells with an integrated filter and growth detection device |
US20050009066A1 (en) * | 2003-05-15 | 2005-01-13 | Connolly Dennis M. | Methods for localizing target molecules in a flowing fluid sample |
CN1860363B (en) * | 2003-08-28 | 2011-12-28 | 赛路拉公司 | Methods and apparatus for sorting cells using an optical switch in a microfluidic channel network |
US7347923B2 (en) * | 2003-10-03 | 2008-03-25 | Sandia Corporation | Dielectrophoresis device and method having insulating ridges for manipulating particles |
US20050287668A1 (en) * | 2003-11-04 | 2005-12-29 | Cell Therapeutics, Inc. (Cti) | RNA interference compositions and screening methods for the identification of novel genes and biological pathways |
US7169560B2 (en) | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
JP2007526823A (en) | 2004-02-02 | 2007-09-20 | ザ ユニバーシティ オブ ブリティッシュ コロンビア | Skodaphoresis and method and apparatus for moving and concentrating particles |
US8529744B2 (en) | 2004-02-02 | 2013-09-10 | Boreal Genomics Corp. | Enrichment of nucleic acid targets |
US10337054B2 (en) | 2004-02-02 | 2019-07-02 | Quantum-Si Incorporated | Enrichment of nucleic acid targets |
ATE463584T1 (en) | 2004-02-19 | 2010-04-15 | Helicos Biosciences Corp | METHOD FOR ANALYZING POLYNUCLEOTIDE SEQUENCES |
ES2348951T3 (en) | 2004-02-26 | 2010-12-17 | Delta Dansk Elektronik, Lys Og Akustik | PROCEDURE, CHIP AND SYSTEM FOR COLLECTION OF BIOLOGICAL PARTICLES. |
ATE436015T1 (en) | 2004-02-26 | 2009-07-15 | Thomsen Bioscience As | METHOD, CHIP, DEVICE AND INTEGRATED SYSTEM FOR DETECTING BIOLOGICAL PARTICLES |
ATE468395T1 (en) * | 2004-02-26 | 2010-06-15 | Thomsen Bioscience As | METHOD, CHIP, DEVICE AND SYSTEM FOR EXTRACTING BIOLOGICAL MATERIALS |
US7187286B2 (en) | 2004-03-19 | 2007-03-06 | Applera Corporation | Methods and systems for using RFID in biological field |
EP1765501A1 (en) | 2004-05-28 | 2007-03-28 | Board of Regents, The University of Texas System | Programmable fluidic processors |
FR2871070B1 (en) * | 2004-06-02 | 2007-02-16 | Commissariat Energie Atomique | MICRODISPOSITIVE AND EMULSION SEPARATION PROCESS |
WO2006017274A2 (en) * | 2004-07-13 | 2006-02-16 | U.S. Genomics, Inc. | Systems and methods for sample modification using fluidic chambers |
ITPD20040301A1 (en) * | 2004-11-26 | 2005-02-26 | Dimensional Srl P | METHOD AND APPARATUS FOR THE SIMULTANEOUS SEPARATION OF BIOLOGICAL MOLECULES BY BIDIMENSIONAL ELECTROPHORESIS |
KR100594408B1 (en) | 2004-12-17 | 2006-06-30 | 한국과학기술연구원 | Device of Separating Cells Using Ultrasound Field and Travelling Wave Dielectrophoresis |
KR100738071B1 (en) * | 2005-01-21 | 2007-07-12 | 삼성전자주식회사 | A dielectrophoresis apparatus disposed of means for concentration gradient generation, method for separating a material and method for screening a suitable conditions for separating a material |
EP1859330B1 (en) | 2005-01-28 | 2012-07-04 | Duke University | Apparatuses and methods for manipulating droplets on a printed circuit board |
CA2496294A1 (en) * | 2005-02-07 | 2006-08-07 | The University Of British Columbia | Apparatus and methods for concentrating and separating particles such as molecules |
JP2006242926A (en) | 2005-03-07 | 2006-09-14 | Seiko Epson Corp | Electrode substrate, detector equipped with the same, kit including detector, detection method using kit |
JP2006242925A (en) | 2005-03-07 | 2006-09-14 | Seiko Epson Corp | Electrode substrate, detector equipped with the substrate, kit including the detector, detection method using the kit |
US20070196820A1 (en) | 2005-04-05 | 2007-08-23 | Ravi Kapur | Devices and methods for enrichment and alteration of cells and other particles |
US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
EP1764418B1 (en) * | 2005-09-14 | 2012-08-22 | STMicroelectronics Srl | Method and device for the treatment of biological samples using dielectrophoresis |
WO2007149111A2 (en) * | 2005-10-06 | 2007-12-27 | Massachusetts Institute Of Technology | Continuous biomolecule separation in a nanofilter |
CN101365781A (en) * | 2005-11-21 | 2009-02-11 | 阿普尔拉公司 | Portable preparation, analysis, and detection apparatus for nucleic acid processing |
KR101157175B1 (en) * | 2005-12-14 | 2012-07-03 | 삼성전자주식회사 | Microfluidic device and method for concentration and lysis of cells or viruses |
JP4748451B2 (en) * | 2006-02-08 | 2011-08-17 | 凸版印刷株式会社 | Hybridization detection method |
WO2007120870A2 (en) * | 2006-04-14 | 2007-10-25 | University Of South Florida | Microbial cell and particle control |
JP4813244B2 (en) * | 2006-04-25 | 2011-11-09 | シャープ株式会社 | Sample separation adsorption device |
US20080050739A1 (en) | 2006-06-14 | 2008-02-28 | Roland Stoughton | Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats |
EP2589668A1 (en) | 2006-06-14 | 2013-05-08 | Verinata Health, Inc | Rare cell analysis using sample splitting and DNA tags |
CA2657621A1 (en) * | 2006-07-14 | 2008-01-17 | Aviva Biosciences Corporation | Methods and compositions for detecting rare cells from a biological sample |
US8999636B2 (en) * | 2007-01-08 | 2015-04-07 | Toxic Report Llc | Reaction chamber |
WO2008108004A1 (en) * | 2007-03-05 | 2008-09-12 | On-Chip Cellomics Consortium | Chip for sampling cell component, system for analyzing cell component and method of analyzing cell component using the same |
US20100294665A1 (en) * | 2007-07-12 | 2010-11-25 | Richard Allen | Method and system for transferring and/or concentrating a sample |
US8277628B2 (en) | 2007-07-13 | 2012-10-02 | The Board Of Trustees Of The Leland Stanford Junior University | Method and apparatus using electric field for improved biological assays |
GB0714866D0 (en) * | 2007-07-31 | 2007-09-12 | Univ Leeds | Biosensor |
US8268246B2 (en) * | 2007-08-09 | 2012-09-18 | Advanced Liquid Logic Inc | PCB droplet actuator fabrication |
US8308926B2 (en) | 2007-08-20 | 2012-11-13 | Purdue Research Foundation | Microfluidic pumping based on dielectrophoresis |
WO2009068862A1 (en) | 2007-11-26 | 2009-06-04 | The Secretary Of State For Innovation, Universities And Skills Of Her Majesty's Britannic Government | Electrochemical detection using silver nanoparticle labelled antibodies |
GB0812679D0 (en) | 2008-07-10 | 2008-08-20 | Sec Dep For Innovation Universities | Sample carrier for effecting chemical assays |
CA2713313A1 (en) * | 2008-02-01 | 2009-08-06 | The University Of British Columbia | Methods and apparatus for particle introduction and recovery |
CN101231262B (en) * | 2008-02-22 | 2011-09-14 | 东南大学 | Method for unicell group logistics |
GB0804491D0 (en) | 2008-03-11 | 2008-04-16 | Iti Scotland Ltd | Detecting analytes |
US20110108422A1 (en) * | 2008-04-03 | 2011-05-12 | The Regents Of The University Of California | Ex vivo multi-dimensional system for the separation and isolation of cells, vesicles, nanoparticles and biomarkers |
EP2288897A2 (en) | 2008-06-06 | 2011-03-02 | University of Washington | Method and system for concentrating particles from a solution |
GB0812681D0 (en) * | 2008-07-10 | 2008-08-20 | Sec Dep For Innovation Universities | Apparatus and methods for effecting chemical assays |
US8361716B2 (en) * | 2008-10-03 | 2013-01-29 | Pathogenetix, Inc. | Focusing chamber |
US8940092B1 (en) | 2008-10-27 | 2015-01-27 | University Of Washington Through Its Center For Commercialization | Hybrid fibers, devices using hybrid fibers, and methods for making hybrid fibers |
US10895575B2 (en) * | 2008-11-04 | 2021-01-19 | Menarini Silicon Biosystems S.P.A. | Method for identification, selection and analysis of tumour cells |
KR101023040B1 (en) | 2008-11-13 | 2011-03-24 | 한국항공대학교산학협력단 | Apparatus for high throughput particle separation and method thereof |
US9134221B2 (en) | 2009-03-10 | 2015-09-15 | The Regents Of The University Of California | Fluidic flow cytometry devices and particle sensing based on signal-encoding |
WO2010121381A1 (en) | 2009-04-21 | 2010-10-28 | The University Of British Columbia | System and methods for detection of particles |
ES2658169T3 (en) * | 2009-08-02 | 2018-03-08 | Qvella Corporation | Cell concentration, capture and lysis devices and their method of use |
KR101885936B1 (en) * | 2010-10-21 | 2018-09-10 | 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 | Microfluidics with Wirelessly Powered Electronic Circuits |
WO2012065075A2 (en) * | 2010-11-12 | 2012-05-18 | The Regents Of The University Of California | Electrokinetic devices and methods for high conductance and high voltage dielectrophoresis (dep) |
US20120219987A1 (en) * | 2010-12-13 | 2012-08-30 | Tofy Mussivand | Device for electroporation and lysis |
CA2742327A1 (en) | 2011-05-20 | 2012-11-20 | The University Of British Columiba | Systems and methods for enhanced scoda |
JP6212488B2 (en) | 2011-07-25 | 2017-10-11 | クヴェッラ コーポレーション | Methods and devices for electrical sample preparation |
US11198126B2 (en) | 2011-10-31 | 2021-12-14 | Fluid-Screen, Inc. | Apparatus for pathogen detection |
US9120105B2 (en) | 2011-10-31 | 2015-09-01 | Monika Weber | Electronic device for pathogen detection |
WO2013104994A2 (en) | 2012-01-13 | 2013-07-18 | The University Of British Columbia | Multiple arm apparatus and methods for separation of particles |
FR2986802A1 (en) * | 2012-02-13 | 2013-08-16 | Centre Nat Rech Scient | Handling and treating cells comprises trapping cells in a network of dielectrophoretic traps that are microfabricated on a substrate, and treating the cells by an electric field applied to the electrodes traps |
US8932815B2 (en) | 2012-04-16 | 2015-01-13 | Biological Dynamics, Inc. | Nucleic acid sample preparation |
US8603791B2 (en) | 2012-04-16 | 2013-12-10 | Biological Dynamics, Inc. | Nucleic acid sample preparation |
US9028776B2 (en) | 2012-04-18 | 2015-05-12 | Toxic Report Llc | Device for stretching a polymer in a fluid sample |
US8685708B2 (en) | 2012-04-18 | 2014-04-01 | Pathogenetix, Inc. | Device for preparing a sample |
WO2013166444A2 (en) | 2012-05-04 | 2013-11-07 | Boreal Genomics Corp. | Biomarker analysis using scodaphoresis |
GB201217390D0 (en) | 2012-09-28 | 2012-11-14 | Agplus Diagnostics Ltd | Test device and sample carrier |
EP2906928A4 (en) | 2012-10-15 | 2016-11-09 | Nanocellect Biomedical Inc | Systems, apparatus, and methods for sorting particles |
US8975060B2 (en) * | 2012-11-30 | 2015-03-10 | Qvella Corporation | Method for pretreatment of microbial samples |
US10662420B2 (en) | 2012-11-30 | 2020-05-26 | Qvella Corporation | Apparatus and method for extracting microbial cells |
US9340835B2 (en) | 2013-03-15 | 2016-05-17 | Boreal Genomics Corp. | Method for separating homoduplexed and heteroduplexed nucleic acids |
CN103627788A (en) * | 2013-08-21 | 2014-03-12 | 中山大学达安基因股份有限公司 | Preparation method for biochip of electrochemical gene sensor |
US9387489B2 (en) | 2014-04-08 | 2016-07-12 | Biological Dynamics, Inc. | Devices for separation of biological materials |
CN103894248B (en) * | 2014-04-09 | 2015-09-16 | 国家纳米科学中心 | A kind of single cell analysis micro-fluidic chip and system and single cell analysis method |
ES2749925T3 (en) | 2014-04-24 | 2020-03-24 | Lucira Health Inc | Colorimetric detection of nucleic acid amplification |
US11130986B2 (en) | 2015-05-20 | 2021-09-28 | Quantum-Si Incorporated | Method for isolating target nucleic acid using heteroduplex binding proteins |
CN104897888A (en) * | 2015-05-27 | 2015-09-09 | 合肥卓元科技服务有限公司 | Separated enzyme reaction sensor system |
CN106310944B (en) * | 2015-07-01 | 2022-10-11 | 王冰 | Dielectrophoresis electrode and electrode array for separating particles and droplets in a medium |
JP6171124B2 (en) * | 2015-10-07 | 2017-08-02 | 株式会社Afiテクノロジー | Inspection device, inspection system, and inspection method |
JP6796932B2 (en) * | 2016-02-16 | 2020-12-09 | 株式会社Afiテクノロジー | Separator |
WO2017160840A1 (en) | 2016-03-14 | 2017-09-21 | Diassess Inc. | Selectively vented biological assay devices and associated methods |
WO2017165852A1 (en) | 2016-03-24 | 2017-09-28 | Biological Dynamics, Inc. | Disposable fluidic cartridge and components |
EP3909686A1 (en) | 2016-04-15 | 2021-11-17 | Fluid-Screen, Inc. | Analyte detection methods and apparatus using dielectrophoresis and electroosmosis |
EP3414010B1 (en) * | 2016-04-22 | 2020-02-26 | Hewlett-Packard Development Company, L.P. | Cell lysis |
JP6671665B2 (en) * | 2016-10-27 | 2020-03-25 | シャープ株式会社 | Fluorescence inspection system, dielectrophoresis device and molecular inspection method |
US10818379B2 (en) | 2017-05-08 | 2020-10-27 | Biological Dynamics, Inc. | Methods and systems for analyte information processing |
US10549275B2 (en) | 2017-09-14 | 2020-02-04 | Lucira Health, Inc. | Multiplexed biological assay device with electronic readout |
JP2021509265A (en) | 2017-12-19 | 2021-03-25 | バイオロジカル ダイナミクス,インク. | Methods and Devices for Detection of Multiple Analyzes from Biological Samples |
JP7317026B2 (en) | 2018-01-30 | 2023-07-28 | ライフ テクノロジーズ コーポレーション | Instruments, devices and consumables for use in smart molecular analysis system workflows |
CN117065932A (en) | 2018-04-02 | 2023-11-17 | 生物动力学公司 | Dielectric material |
USD907232S1 (en) | 2018-12-21 | 2021-01-05 | Lucira Health, Inc. | Medical testing device |
JP2022523964A (en) * | 2019-03-05 | 2022-04-27 | ルシラ ヘルス インコーポレイテッド | Air bubble-free, fluid chamber liquid filling method |
WO2021097035A1 (en) | 2019-11-13 | 2021-05-20 | Fluid-Screen, Inc. | An apparatus and methods to rapidly detect, separate, purify, and quantify various viruses from cells, cultured medium and other fluids |
CA3161454A1 (en) | 2019-11-13 | 2021-05-20 | Fluid-Screen, Inc. | Methods and apparatus for detection of bacteria in a sample using dielectrophoresis |
US11542541B2 (en) | 2019-11-26 | 2023-01-03 | Bio-Rad Laboratories, Inc. | Method and system for sampling material from cells |
CN111250183B (en) * | 2020-02-17 | 2021-04-09 | 北京中科生仪科技有限公司 | Liquid injection pump driving device for micro-fluidic system |
USD953561S1 (en) | 2020-05-05 | 2022-05-31 | Lucira Health, Inc. | Diagnostic device with LED display |
CN111644212B (en) * | 2020-05-22 | 2022-05-24 | 华东理工大学 | Micro-fluidic chip and nano-particle separation device |
USD962470S1 (en) | 2020-06-03 | 2022-08-30 | Lucira Health, Inc. | Assay device with LCD display |
WO2022020206A1 (en) * | 2020-07-24 | 2022-01-27 | Palogen, Inc. | Nanochannel systems and methods for detecting pathogens using same |
CN112986556B (en) * | 2021-05-12 | 2022-02-18 | 佛山微奥云生物技术有限公司 | Chip for rapid detection and processing method thereof |
Family Cites Families (82)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3346479A (en) * | 1964-04-09 | 1967-10-10 | Scientific Industries | Preparative separation by a combination of gel separation and electrophoresis |
US3375187A (en) * | 1965-05-13 | 1968-03-26 | Buchler Instr Inc | Apparatus for temperature controlled preparative and analytical electrophoresis |
US3533933A (en) * | 1967-03-31 | 1970-10-13 | Hannig Kurt | Process and device for the isolation of fractions of a substance mixture electrophoretically separated in a carrier gel |
US3539493A (en) * | 1967-08-31 | 1970-11-10 | Canal Ind Corp | Apparatus for preparative electrophoresis on gel support media |
US3627137A (en) * | 1968-10-10 | 1971-12-14 | Milan Bier | Dialyzing membrane envelope |
DE1807481C3 (en) * | 1968-11-07 | 1975-10-09 | Messerschmitt-Boelkow-Blohm Gmbh, 8000 Muenchen | Partial electroplating process |
US3616454A (en) * | 1969-03-13 | 1971-10-26 | Univ New York | Method of and apparatus for electrophoretic separation in a gel column |
US3640813A (en) * | 1969-06-09 | 1972-02-08 | Samuel T Nerenberg | Adapter for a macromolecule separation device |
DE2051715B2 (en) | 1970-10-21 | 1976-01-15 | SEPARATION CHAMBER FOR CONTINUOUS DEFLECTION ELECTROPHORESIS | |
JPS4821679U (en) | 1971-07-16 | 1973-03-12 | ||
NL7115364A (en) * | 1971-11-08 | 1973-05-10 | ||
DE2221242C3 (en) * | 1972-04-29 | 1974-09-26 | Stephan 8520 Erlangen Nees | Apparatus for preparative electrophoresis and procedures for carrying it out with this apparatus |
SU434985A1 (en) | 1972-06-07 | 1974-07-05 | DEVICE FOR PREPARATIVE SEPARATION OF HIGH-MOLECULAR BIOLOGICAL SUBSTANCES | |
GB1359944A (en) | 1972-07-18 | 1974-07-17 | Miles Lab | Apparatus for gel electrophoresis |
US3795600A (en) * | 1973-03-23 | 1974-03-05 | Instrumentation Specialties Co | Electrophoresis and method apparatus |
US3980546A (en) * | 1975-06-27 | 1976-09-14 | Caccavo Francis A | Micropreparative electrophoresis apparatus |
NL182822C (en) * | 1976-05-18 | 1988-05-16 | Roskam Willem Gerrit | PREPARATIVE ELECTROFORESE DEVICE. |
SU616568A1 (en) | 1977-01-24 | 1978-07-25 | Специальное Конструкторское Бюро Биофизической Аппаратуры | Apparatus for electro-phoretic separation of substances |
US4326934A (en) * | 1979-12-31 | 1982-04-27 | Pohl Herbert A | Continuous dielectrophoretic cell classification method |
US4390403A (en) * | 1981-07-24 | 1983-06-28 | Batchelder J Samuel | Method and apparatus for dielectrophoretic manipulation of chemical species |
JPS58147639A (en) | 1982-02-26 | 1983-09-02 | Hideyuki Nishizawa | Separation by continuous electric migration and its device |
DE3374082D1 (en) * | 1982-07-23 | 1987-11-19 | Matthias Hediger | Apparatus for preparative gel electrophoresis, in particular for separating mixtures on a gradient gel polyacryl amide column |
US4441972A (en) * | 1983-04-08 | 1984-04-10 | D.E.P. Systems, Inc. | Apparatus for electrofusion of biological particles |
US4661451A (en) * | 1984-02-06 | 1987-04-28 | Ortho Diagnostic Systems, Inc. | Methods for immobilizing and translocating biological cells |
EP0155833A3 (en) * | 1984-03-15 | 1988-07-27 | Fuji Photo Film Co., Ltd. | Element for electrophoresis |
FR2574311B1 (en) | 1984-12-11 | 1989-09-15 | Inst Nat Sante Rech Med | IMPROVEMENTS TO PREPARATIVE ELECTROPHORESIS PROCESSES AND APPARATUS |
US4617102A (en) * | 1985-03-21 | 1986-10-14 | Lifecodes Corp. | Process and apparatus for purifying and concentrating DNA from crude mixtures containing DNA |
US4740283A (en) * | 1986-02-27 | 1988-04-26 | University Patents, Inc. | Pulsed-field gradient gel electrophoretic apparatus |
US4699706A (en) * | 1986-05-30 | 1987-10-13 | Bio-Rad Laboratories, Inc. | Method and apparatus for extraction of species from electrophoresis media |
US5593580A (en) * | 1986-11-26 | 1997-01-14 | Kopf; Henry B. | Filtration cassette article, and filter comprising same |
DE3876273T2 (en) * | 1987-04-11 | 1993-05-27 | Ciba Geigy Ag | ISOELECTRIC FOCUSING METHOD AND DEVICE FOR CARRYING OUT THIS METHOD. |
US4787963A (en) * | 1987-05-04 | 1988-11-29 | Syntro Corporation | Method and means for annealing complementary nucleic acid molecules at an accelerated rate |
US4936963A (en) * | 1987-05-27 | 1990-06-26 | Abbott Laboratories | Polycationic buffers and method for gel electrophoresis of nucleic acids |
US5202010A (en) | 1987-11-25 | 1993-04-13 | Princeton Biochemicals, Inc. | Automated capillary electrophoresis apparatus |
US6054270A (en) * | 1988-05-03 | 2000-04-25 | Oxford Gene Technology Limited | Analying polynucleotide sequences |
US4908112A (en) * | 1988-06-16 | 1990-03-13 | E. I. Du Pont De Nemours & Co. | Silicon semiconductor wafer for analyzing micronic biological samples |
US5382511A (en) * | 1988-08-02 | 1995-01-17 | Gene Tec Corporation | Method for studying nucleic acids within immobilized specimens |
US5188963A (en) * | 1989-11-17 | 1993-02-23 | Gene Tec Corporation | Device for processing biological specimens for analysis of nucleic acids |
US4877510A (en) * | 1988-10-25 | 1989-10-31 | Bio-Rad Laboratories, Inc. | Apparatus for preparative gel electrophoresis |
US5062942A (en) * | 1989-04-12 | 1991-11-05 | Hitachi, Ltd. | Fluorescence detection type electrophoresis apparatus |
US5143854A (en) * | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
US5344535A (en) * | 1989-11-27 | 1994-09-06 | British Technology Group Limited | Dielectrophoretic characterization of micro-organisms and other particles |
US5795457A (en) * | 1990-01-30 | 1998-08-18 | British Technology Group Ltd. | Manipulation of solid, semi-solid or liquid materials |
US5126022A (en) * | 1990-02-28 | 1992-06-30 | Soane Tecnologies, Inc. | Method and device for moving molecules by the application of a plurality of electrical fields |
US5085756A (en) * | 1990-08-21 | 1992-02-04 | Hewlett-Packard Company | Column separation system for electrophoresis with sample pretreatment |
US5527670A (en) * | 1990-09-12 | 1996-06-18 | Scientific Generics Limited | Electrochemical denaturation of double-stranded nucleic acid |
US5269931A (en) * | 1990-09-17 | 1993-12-14 | Gelman Sciences Inc. | Cationic charge modified microporous membranes |
US5151189A (en) * | 1990-09-17 | 1992-09-29 | Gelman Sciences, Inc. | Cationic charge modified microporous membrane |
US5340449A (en) * | 1990-12-07 | 1994-08-23 | Shukla Ashok K | Apparatus for electroelution |
JP3104995B2 (en) * | 1991-01-31 | 2000-10-30 | ワーナー−ランバート・コンパニー | Substituted heteroaryl analogs of 4,6-di-tert-butyl-5-hydroxy-1,3-pyrimidine useful as anti-inflammatory agents |
US5217593A (en) * | 1991-03-13 | 1993-06-08 | Macconnell William P | Nucleic acid purification system and method |
US5139637A (en) * | 1991-03-13 | 1992-08-18 | Macconnell William P | Plasmid purification system and method |
US5078853A (en) * | 1991-03-22 | 1992-01-07 | Assay Technology, Inc. | Segmented electrophoretic separation system useful for lipid profiling |
US5209831A (en) * | 1991-06-14 | 1993-05-11 | Macconnell William P | Bufferless electrophoresis system and method |
US5161165A (en) * | 1991-09-26 | 1992-11-03 | Hewlett-Packard Company | Multimode stabilized external cavity laser |
US5632957A (en) * | 1993-11-01 | 1997-05-27 | Nanogen | Molecular biological diagnostic systems including electrodes |
US5849486A (en) * | 1993-11-01 | 1998-12-15 | Nanogen, Inc. | Methods for hybridization analysis utilizing electrically controlled hybridization |
US6051380A (en) * | 1993-11-01 | 2000-04-18 | Nanogen, Inc. | Methods and procedures for molecular biological analysis and diagnostics |
US5605662A (en) * | 1993-11-01 | 1997-02-25 | Nanogen, Inc. | Active programmable electronic devices for molecular biological analysis and diagnostics |
US6048690A (en) * | 1991-11-07 | 2000-04-11 | Nanogen, Inc. | Methods for electronic fluorescent perturbation for analysis and electronic perturbation catalysis for synthesis |
US6017696A (en) * | 1993-11-01 | 2000-01-25 | Nanogen, Inc. | Methods for electronic stringency control for molecular biological analysis and diagnostics |
US5846708A (en) * | 1991-11-19 | 1998-12-08 | Massachusetts Institiute Of Technology | Optical and electrical methods and apparatus for molecule detection |
EP0544969B1 (en) | 1991-12-06 | 1997-03-05 | Ciba-Geigy Ag | Apparatus and method for electrophoretic separation |
JPH05236997A (en) * | 1992-02-28 | 1993-09-17 | Hitachi Ltd | Chip for catching polynucleotide |
GB9208357D0 (en) * | 1992-04-16 | 1992-06-03 | British Tech Group | Apparatus for separating a mixture |
US5340499A (en) | 1992-08-11 | 1994-08-23 | Neste Oy | Electrically conductive compositions and methods for their preparation |
US5376249A (en) * | 1992-11-25 | 1994-12-27 | Perseptive Biosystems, Inc. | Analysis utilizing isoelectric focusing |
GB9301122D0 (en) * | 1993-01-21 | 1993-03-10 | Scient Generics Ltd | Method of analysis/separation |
GB9306729D0 (en) * | 1993-03-31 | 1993-05-26 | British Tech Group | Improvements in separators |
US5427664A (en) * | 1993-07-22 | 1995-06-27 | Stoev; Stoyan V. | Free solution electrophoresis-membrane filters trapping assay apparatus and method |
US6309601B1 (en) * | 1993-11-01 | 2001-10-30 | Nanogen, Inc. | Scanning optical detection system |
US6726880B1 (en) * | 1993-11-01 | 2004-04-27 | Nanogen, Inc. | Electronic device for performing active biological operations and method of using same |
US6099803A (en) * | 1994-07-07 | 2000-08-08 | Nanogen, Inc. | Advanced active electronic devices for molecular biological analysis and diagnostics |
US6254827B1 (en) * | 1993-11-01 | 2001-07-03 | Nanogen, Inc. | Methods for fabricating multi-component devices for molecular biological analysis and diagnostics |
DE4400955C2 (en) * | 1993-12-23 | 1999-04-01 | Fraunhofer Ges Forschung | Adhesion-controllable surface structure |
US6071394A (en) * | 1996-09-06 | 2000-06-06 | Nanogen, Inc. | Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis |
US5728267A (en) * | 1994-11-15 | 1998-03-17 | Flaherty; James E. | Concentrator for separating small samples in a centrifuge |
EP0846161B1 (en) * | 1995-08-25 | 2005-08-17 | Affymetrix, Inc. (a Delaware Corporation) | Release of intracellular material |
US5858192A (en) * | 1996-10-18 | 1999-01-12 | Board Of Regents, The University Of Texas System | Method and apparatus for manipulation using spiral electrodes |
EP1210607A4 (en) | 1999-08-13 | 2004-12-22 | Nanogen Inc | Microelectronic molecular descriptor array devices, methods, procedures, and formats for combinatorial selection of intermolecular ligand binding structures and for drug screening |
US6960298B2 (en) * | 2001-12-10 | 2005-11-01 | Nanogen, Inc. | Mesoporous permeation layers for use on active electronic matrix devices |
US20040011650A1 (en) * | 2002-07-22 | 2004-01-22 | Frederic Zenhausern | Method and apparatus for manipulating polarizable analytes via dielectrophoresis |
-
1998
- 1998-01-30 US US09/016,596 patent/US6071394A/en not_active Expired - Lifetime
-
1999
- 1999-01-26 KR KR10-2000-7008329A patent/KR100512518B1/en not_active IP Right Cessation
- 1999-01-26 CA CA002319705A patent/CA2319705C/en not_active Expired - Fee Related
- 1999-01-26 DE DE69909971T patent/DE69909971D1/en not_active Expired - Lifetime
- 1999-01-26 BR BR9908349-3A patent/BR9908349A/en not_active IP Right Cessation
- 1999-01-26 CN CNB99803469XA patent/CN1170942C/en not_active Expired - Fee Related
- 1999-01-26 EP EP99903423A patent/EP1053055B1/en not_active Expired - Lifetime
- 1999-01-26 JP JP2000529896A patent/JP2002502047A/en active Pending
- 1999-01-26 WO PCT/US1999/001651 patent/WO1999038612A1/en active IP Right Grant
- 1999-01-26 AU AU23445/99A patent/AU743074B2/en not_active Ceased
- 1999-01-26 AT AT99903423T patent/ATE246040T1/en not_active IP Right Cessation
- 1999-01-26 NZ NZ505858A patent/NZ505858A/en unknown
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2000
- 2000-04-13 US US09/548,522 patent/US6280590B1/en not_active Expired - Lifetime
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2001
- 2001-07-13 US US09/905,755 patent/US6989086B2/en not_active Expired - Fee Related
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WO1999038612A1 (en) | 1999-08-05 |
BR9908349A (en) | 2000-12-05 |
NZ505858A (en) | 2002-09-27 |
CN1170942C (en) | 2004-10-13 |
US6989086B2 (en) | 2006-01-24 |
CN1291913A (en) | 2001-04-18 |
US6071394A (en) | 2000-06-06 |
KR20010040474A (en) | 2001-05-15 |
AU2344599A (en) | 1999-08-16 |
DE69909971D1 (en) | 2003-09-04 |
JP2002502047A (en) | 2002-01-22 |
EP1053055B1 (en) | 2003-07-30 |
US6280590B1 (en) | 2001-08-28 |
US20010045359A1 (en) | 2001-11-29 |
EP1053055A1 (en) | 2000-11-22 |
ATE246040T1 (en) | 2003-08-15 |
KR100512518B1 (en) | 2005-09-06 |
AU743074B2 (en) | 2002-01-17 |
CA2319705C (en) | 2008-07-15 |
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