CA2324208A1 - Vascularized perfused microtissue/micro-organ arrays - Google Patents

Vascularized perfused microtissue/micro-organ arrays Download PDF

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Publication number
CA2324208A1
CA2324208A1 CA002324208A CA2324208A CA2324208A1 CA 2324208 A1 CA2324208 A1 CA 2324208A1 CA 002324208 A CA002324208 A CA 002324208A CA 2324208 A CA2324208 A CA 2324208A CA 2324208 A1 CA2324208 A1 CA 2324208A1
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Prior art keywords
cells
matrix
materials
perfusion
organ
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CA002324208A
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French (fr)
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CA2324208C (en
Inventor
Linda G. Griffith
Steven R. Tannenbaum
Mark J. Powers
Karel Domansky
Charles D. Thompson
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Massachusetts Institute of Technology
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Massachusetts Institute Of Technology
Linda G. Griffith
Steven R. Tannenbaum
Mark J. Powers
Karel Domansky
Charles D. Thompson
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Application filed by Massachusetts Institute Of Technology, Linda G. Griffith, Steven R. Tannenbaum, Mark J. Powers, Karel Domansky, Charles D. Thompson filed Critical Massachusetts Institute Of Technology
Publication of CA2324208A1 publication Critical patent/CA2324208A1/en
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    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
    • C09K8/60—Compositions for stimulating production by acting on the underground formation
    • C09K8/62—Compositions for forming crevices or fractures
    • C09K8/66—Compositions based on water or polar solvents
    • C09K8/68—Compositions based on water or polar solvents containing organic compounds
    • C09K8/685—Compositions based on water or polar solvents containing organic compounds containing cross-linking agents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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    • G01N33/5064—Endothelial cells
    • G—PHYSICS
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    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00286—Reactor vessels with top and bottom openings
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Abstract

Systems including (1) a micromatrix and perfusion assembly suitable for seeding and attachment of cells within the matrix and for morphogenesis of seeded cells into complex, hierarchical tissue or organ structures, wherein the matrix includes channels or vessels through which culture medium, oxygen, or other nutrient or body fluids can be perfused while controlling gradients of nutrients and exogenous metabolites throughout the perfusion path independently of perfusion rate, and (2) sensor means for detecting changes in either cells within the matrix or in materials exposed to the cells, have been developed. Methods for making the micromatrices include micromachining, micromolding, embossing, laser drilling, and electro deposition machining.
Cells can be of one or more types, either differentiated or undifferentiated. In a preferred embodiment, the matrix is seeded with a mixture of cells including endothelial cells which will line the channels to form "blood vessels", and at least one type of parenchymal cells, such as hepatocytes, pancreatic cells, or other organ cells.
The system can be used to screen materials for an effect on the cells, for an effect of the cells on the materials (for example, in a manner equivalent to tissue metabolism of a drug), or to test a material on a biological that must first infect cells or tissues, such as viruses. The apparatus also can be used to provide a physiological environment for expansion of stem cells, or for enabling gene therapy in vitro.
CA002324208A 1998-03-18 1999-03-18 Vascularized perfused microtissue/micro-organ arrays Expired - Lifetime CA2324208C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US7845298P 1998-03-18 1998-03-18
US60/078,452 1998-03-18
PCT/US1999/005974 WO1999047922A2 (en) 1998-03-18 1999-03-18 Vascularized perfused microtissue/micro-organ arrays

Publications (2)

Publication Number Publication Date
CA2324208A1 true CA2324208A1 (en) 1999-09-23
CA2324208C CA2324208C (en) 2009-06-30

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CA002324208A Expired - Lifetime CA2324208C (en) 1998-03-18 1999-03-18 Vascularized perfused microtissue/micro-organ arrays

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US (1) US6197575B1 (en)
EP (1) EP1064353B1 (en)
AT (1) ATE227338T1 (en)
CA (1) CA2324208C (en)
DE (1) DE69903800T2 (en)
WO (1) WO1999047922A2 (en)

Cited By (1)

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Publication number Priority date Publication date Assignee Title
CN110352239A (en) * 2016-12-23 2019-10-18 银丝佛若有限公司 The liver diseases model and method that can be screened

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US6424852B1 (en) * 1996-10-18 2002-07-23 Lucid, Inc. System for confocal imaging within dermal tissue
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