CA2331263A1 - A method of producing cells with a desired property by recursive sequence recombination - Google Patents
A method of producing cells with a desired property by recursive sequence recombination Download PDFInfo
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- CA2331263A1 CA2331263A1 CA002331263A CA2331263A CA2331263A1 CA 2331263 A1 CA2331263 A1 CA 2331263A1 CA 002331263 A CA002331263 A CA 002331263A CA 2331263 A CA2331263 A CA 2331263A CA 2331263 A1 CA2331263 A1 CA 2331263A1
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
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- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
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- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/16—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing two or more hetero rings
- C12P17/162—Heterorings having oxygen atoms as the only ring heteroatoms, e.g. Lasalocid
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
Abstract
The invention provides methods employing iterative cycles of recombination a nd selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.
Claims (42)
1. A method of producing a library of diverse multicellular organsims, the method comprising:
providing a pool of male gametes and a pool of female gametes, wherein at least one of the male pool or the female pool comprises a plurality of different gametes derived from different strains of a species or different species, wherein the male gametes fertilize the female gametes;
permitting at least a portion of the resulting fertilized gametes to grow into reproductively viable organisms;
repeatedly crossing the reproductively viable organisms to produce a library of diverse organisms; and, selecting the library for a desired trait or property.
providing a pool of male gametes and a pool of female gametes, wherein at least one of the male pool or the female pool comprises a plurality of different gametes derived from different strains of a species or different species, wherein the male gametes fertilize the female gametes;
permitting at least a portion of the resulting fertilized gametes to grow into reproductively viable organisms;
repeatedly crossing the reproductively viable organisms to produce a library of diverse organisms; and, selecting the library for a desired trait or property.
2. The method of claim 1, wherein the library of diverse organisms comprise a plurality of plants.
3. The method of claim 2, wherein the plants are selected from: Gramineae, Fetucoideae, Poacoideae, Agrostis, Phleum, Dactylis, Sorgum, Setaria, Zea, Oryza, Triticum, Secale, Avena, Hordeum, Saccharum, Poa, Festuca, Stenotaphrum, Cynodon, Coix, Olyreae, Phareae, Compositae, and leguminosae.
4. The method of claim 2, wherein the plants are selected from corn), rice, wheat, rye, oats, barley, pea, beans, lentil, peanut, yam bean, cowpeas, velvet beans, soybean, clover, alfalfa, lupine, vetch, lotus, sweet clover, wisteria, sweetpea, sorghum, millet, sunflower, and canola.
5. The method of claim 1, wherein the library of diverse organisms comprise a plurality of animals.
6. The method of claim 5, wherein the animals are selected from non-human mammals and fish.
7. The library produced by the method of claim 1.
8. The method of claim 1, further comprising:
crossing a plurality of selected library members by pooling gametes from the selected members and repeatedly crossing any resulting additional reproductively viable organisms to produce a second library of diverse organisms; and, selecting the second library for a desired trait or property.
crossing a plurality of selected library members by pooling gametes from the selected members and repeatedly crossing any resulting additional reproductively viable organisms to produce a second library of diverse organisms; and, selecting the second library for a desired trait or property.
9. The second library made by the method of claim 8.
10. A method of evolving a cell to acquire a desired property, comprising:
(i.) forming protoplasts of a population of different cells;
(ii.) fusing the protoplasts to form hybrid protoplasts, in which genomes from the protoplasts recombine to form hybrid genomes;
(iii.) incubating the hybrid protoplasts under conditions promoting regeneration of cells, thereby producing regenerated cells;
(iv.) repeatedly forming protoplasts from the regenerated cells, fusing the protoplasts to form hybrid protoplasts, in which genomes from the protoplasts recombine to form additional hybrid genomes; incubating the additional hybrid protoplasts under conditions promoting regeneration of cells, thereby producing additional regenerated cells; and, (v.) selecting or screening to isolate regenerated cells or additionally regenerated cells that have evolved toward acquisition of the desired property.
(i.) forming protoplasts of a population of different cells;
(ii.) fusing the protoplasts to form hybrid protoplasts, in which genomes from the protoplasts recombine to form hybrid genomes;
(iii.) incubating the hybrid protoplasts under conditions promoting regeneration of cells, thereby producing regenerated cells;
(iv.) repeatedly forming protoplasts from the regenerated cells, fusing the protoplasts to form hybrid protoplasts, in which genomes from the protoplasts recombine to form additional hybrid genomes; incubating the additional hybrid protoplasts under conditions promoting regeneration of cells, thereby producing additional regenerated cells; and, (v.) selecting or screening to isolate regenerated cells or additionally regenerated cells that have evolved toward acquisition of the desired property.
11. The method of claim 10, wherein the desired property is selected from:
heat tolerance, ethanol production, ethanol tolerance, acid, improved production and maintenance of enzyme cofactors, improved production and maintenance of NAD(P)H, and improved glucose transport.
heat tolerance, ethanol production, ethanol tolerance, acid, improved production and maintenance of enzyme cofactors, improved production and maintenance of NAD(P)H, and improved glucose transport.
12. The method of claim 10, further comprising repeating steps (i.)-(v.) with regenerated cells in step (iii.) or additional regenerated cells in step (iv.) being used to form the protoplasts in step (i.) until the regenerated cells have acquired the desired property.
13. The method of claim 10, comprising step (iv), wherein step (iv). is performed prior to step (v.).
14. The method of claim 10, wherein the hybrid protoplasts comprise cells having more than two parental genomes.
15. The method of claim 10, wherein the different cells are fungal cells, and the regenerated cells are fungi mycelia.
16. The method of claim 15, wherein protoplasts are provided by treating mycelia or spores with an enzyme.
17. The method of claim 15, wherein the fungal cells are from a fragile strain, lacking capacity for intact cell wall synthesis; whereby protoplast form spontaneously.
18. The method of claim 15, further comprising treating the mycelia with an inhibitor of cell wall formation to generate protoplasts.
19. The method of claim 10, further comprising selecting or screening to isolate regenerated cells with hybrid genomes free from cells with parental genomes.
20. The method of claim 10, wherein a first subpopulation of cells contain a first marker and the second subpopulation of cells contain a second marker, and the method further comprising selecting or screening to identify regenerated cells expressing both the first and second marker.
21. The method of claim 10, wherein the first marker is a membrane marker and the second marker is a genetic marker.
22. The method of claim 10, wherein the first marker is a first subunit of a heteromeric enzyme and the second marker is a second subunit of the heteromeric enzyme.
23. The method of claim 10, fiuther comprising transforming protoplasts with a library of DNA fragments in at least one cycle.
24. The method of claim 23, wherein the DNA fragments are accompanied by a restriction enzyme.
25. The method of claim 10, further comprising exposing the protoplasts to ultraviolet irradiation in at least one cycle.
26. The method of claim 10, wherein the desired property is the expression of a protein, primary metabolite, or secondary metabolite.
27. The method of claim 10, wherein the desired property is the secretion of a protein or secondary metabolite.
28. The method of claim 27, wherein the secondary metabolite is selected from taxol, cyclosporin A, and erythromycin.
29. The method of claim 10, wherein the desired property is capacity for meiosis.
30. The method of claim 10, wherein the desired property is compatibility to form a heterokaryon with another strain.
31. The method of claim 10, further comprising exposing the protoplasts or mycelia to a mutagenic agent in at least one cycle.
32. A method for whole genome shuffling through organized heteroduplex shining, the method comprising:
(a). providing chromosomal DNA of an organism which is targeted for shuffling, digesting the chromosomal DNA with one or more restriction enzymes, ligating the chromosomal DNA into a cosmid, the cosmid comprising at least two rare restriction enzyme recognition sites, aliquoting, purifying, and storing sufficient cosmids to represent a complete chromosome;
(b). mutagenizing aliquots of the library in vitro using a mutagen;
(c). transfecting a sample from a plurality of the mutagenized aliquots into a population of target cells;
(d). assaying resulting transfectants for phenotypic improvements;
(e). growing transfected cells harboring a mutant library of the identified cosmid(s) on media and screening the resulting cell colonies for independent mutants conferring an desired phenotype;
(f). isolating and pooling DNA from cells identified in the screening;
(g). dividing the selected pools and digesting at least one sample with a rare-cutting restriction enzyme, pooling the cleaved samples, denaturing the samples, reannealing the samples and religating the samples; and, (h). transfecting target cells with the resulting heteroduplexes and propagating the cells to allow recombination to occur between the strands of the heteroduplexes in vivo.
(a). providing chromosomal DNA of an organism which is targeted for shuffling, digesting the chromosomal DNA with one or more restriction enzymes, ligating the chromosomal DNA into a cosmid, the cosmid comprising at least two rare restriction enzyme recognition sites, aliquoting, purifying, and storing sufficient cosmids to represent a complete chromosome;
(b). mutagenizing aliquots of the library in vitro using a mutagen;
(c). transfecting a sample from a plurality of the mutagenized aliquots into a population of target cells;
(d). assaying resulting transfectants for phenotypic improvements;
(e). growing transfected cells harboring a mutant library of the identified cosmid(s) on media and screening the resulting cell colonies for independent mutants conferring an desired phenotype;
(f). isolating and pooling DNA from cells identified in the screening;
(g). dividing the selected pools and digesting at least one sample with a rare-cutting restriction enzyme, pooling the cleaved samples, denaturing the samples, reannealing the samples and religating the samples; and, (h). transfecting target cells with the resulting heteroduplexes and propagating the cells to allow recombination to occur between the strands of the heteroduplexes in vivo.
33. The method of claim 32, further comprising additionally screening the transfectants.
34. The method of claim 32, further comprising further shuffling the heteroduplexes by recursive in vitro heteroduplex formation and in vivo recombination prior to additionally screening the transfectants.
35. The method of claim 33, further comprising performing an additional mutagenesis step to increase diversity during the shuffling process.
36. The method of claim 32, further comprising combining one or more heteroduplexes into a host chromosome by chromosome integration.
37. The method of claim 36, further comprising repeating steps (a).-(h)., using the organism resulting from chromosome integration as the source for chromosomal DNA in step (a).
38. The method of claim 32, wherein the cosmid comprises restriction sites for Sfr or NotI.
39. The method of claim 32, wherein the transfectants are assayed as a pool from each mutagenized aliquot.
40. The method of claim 32, wherein a positive assay result indicates that a cosmid from a particular aliquot can confer phenotypic improvements and contains large genomic fragments that are suitable targets for heteroduplex mediated shuffling.
41. The method of claim 32, wherein the mutagen is a chemical mutagen.
42. The method of claim 32, wherein growing transfected cells harboring a mutant library of the identified cosmid(s) on media comprises plating the transfected cells on solid media.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CA2720311A CA2720311A1 (en) | 1998-07-15 | 1999-07-15 | A method of producing cells with a desired property by recursive sequence recombination |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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US09/116,188 US6326204B1 (en) | 1997-01-17 | 1998-07-15 | Evolution of whole cells and organisms by recursive sequence recombination |
US09/116,188 | 1998-07-15 | ||
PCT/US1999/015972 WO2000004190A1 (en) | 1998-07-15 | 1999-07-15 | Evolution of whole cells and organisms by recursive sequence recombination |
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CA2720311A Division CA2720311A1 (en) | 1998-07-15 | 1999-07-15 | A method of producing cells with a desired property by recursive sequence recombination |
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CA2331263A1 true CA2331263A1 (en) | 2000-01-27 |
CA2331263C CA2331263C (en) | 2011-01-18 |
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CA2720311A Abandoned CA2720311A1 (en) | 1998-07-15 | 1999-07-15 | A method of producing cells with a desired property by recursive sequence recombination |
CA2331263A Expired - Fee Related CA2331263C (en) | 1998-07-15 | 1999-07-15 | A method of producing cells with a desired property by recursive sequence recombination |
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CA2720311A Abandoned CA2720311A1 (en) | 1998-07-15 | 1999-07-15 | A method of producing cells with a desired property by recursive sequence recombination |
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US (6) | US6326204B1 (en) |
EP (2) | EP1707641A3 (en) |
JP (2) | JP4674329B2 (en) |
KR (1) | KR100647112B1 (en) |
AT (1) | ATE317453T1 (en) |
AU (1) | AU771511B2 (en) |
CA (2) | CA2720311A1 (en) |
DE (1) | DE69929796T2 (en) |
ES (1) | ES2257866T3 (en) |
IL (1) | IL140121A0 (en) |
WO (1) | WO2000004190A1 (en) |
Families Citing this family (230)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
US6335160B1 (en) * | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
US7148054B2 (en) * | 1997-01-17 | 2006-12-12 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
US6326204B1 (en) | 1997-01-17 | 2001-12-04 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
AU1124499A (en) | 1997-10-28 | 1999-05-17 | Maxygen, Inc. | Human papillomavirus vectors |
US6596539B1 (en) | 1997-10-31 | 2003-07-22 | Maxygen, Inc. | Modification of virus tropism and host range by viral genome shuffling |
US6146894A (en) | 1998-04-14 | 2000-11-14 | The Johns Hopkins University | Method for generating hypermutable organisms |
US6902918B1 (en) | 1998-05-21 | 2005-06-07 | California Institute Of Technology | Oxygenase enzymes and screening method |
US7153655B2 (en) | 1998-06-16 | 2006-12-26 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence |
AU4441699A (en) | 1998-06-17 | 2000-01-05 | Maxygen, Inc. | Method for producing polynucleotides with desired properties |
BR9911681A (en) * | 1998-08-12 | 2001-10-02 | Maxygen Inc | DNA shuffling to produce selective crops for herbicides |
US6991922B2 (en) * | 1998-08-12 | 2006-01-31 | Proteus S.A. | Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation |
US20030104417A1 (en) * | 1998-08-12 | 2003-06-05 | Proteus S.A. | Template-mediated, ligation-oriented method of nonrandomly shuffling polynucleotides |
US6951719B1 (en) | 1999-08-11 | 2005-10-04 | Proteus S.A. | Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained |
US20060242731A1 (en) * | 1998-08-12 | 2006-10-26 | Venkiteswaran Subramanian | DNA shuffling to produce herbicide selective crops |
US20040023213A1 (en) * | 1998-08-12 | 2004-02-05 | Pangene Corporation | Domain specific gene evolution |
US20030092023A1 (en) * | 1998-08-12 | 2003-05-15 | Daniel Dupret | Method of shuffling polynucleotides using templates |
US20040191772A1 (en) * | 1998-08-12 | 2004-09-30 | Dupret Daniel Marc | Method of shuffling polynucleotides using templates |
US6299850B1 (en) * | 1999-03-16 | 2001-10-09 | The United States Of America As Represented By The Department Of Energy | Carbon activation process for increased surface accessibility in electrochemical capacitors |
US7270969B2 (en) * | 1999-05-05 | 2007-09-18 | Phylogica Limited | Methods of constructing and screening diverse expression libraries |
US7033781B1 (en) | 1999-09-29 | 2006-04-25 | Diversa Corporation | Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating |
US7026119B2 (en) | 2000-02-11 | 2006-04-11 | Morphotek, Inc. | Methods for generating hypermutable microbes |
DE60039371D1 (en) | 2000-02-18 | 2008-08-14 | Morphotek Inc | METHOD FOR THE PRODUCTION OF HYPERMUTABLE PLANTS |
DE60126065T2 (en) * | 2000-02-23 | 2007-08-30 | Morphotek Inc. | METHOD OF GENERATING FAST MUTIER YEAST |
US6825038B2 (en) | 2000-05-12 | 2004-11-30 | The Johns Hopkins University | Method for generating hypermutable organisms |
US7115403B1 (en) | 2000-05-16 | 2006-10-03 | The California Institute Of Technology | Directed evolution of galactose oxidase enzymes |
EP1294869A2 (en) * | 2000-06-14 | 2003-03-26 | Diversa Corporation | Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating |
US20020072097A1 (en) * | 2000-07-07 | 2002-06-13 | Delcardayre Stephen | Molecular breeding of transposable elements |
US7435562B2 (en) | 2000-07-21 | 2008-10-14 | Modular Genetics, Inc. | Modular vector systems |
DK1309677T4 (en) * | 2000-08-11 | 2012-06-25 | Genencor Int | Bac illustration transformation, transformants and mutant libraries |
US6991786B1 (en) * | 2000-08-30 | 2006-01-31 | Wisconsin Alumni Research Foundation | Anti-microbial biotherapeutic agents: alternatives to conventional pharmaceutical antibiotics |
EP1319081A2 (en) * | 2000-09-18 | 2003-06-18 | Pioneer Hi-Bred International, Inc. | Rice mlh1 ortholog and uses thereof |
US7083945B1 (en) * | 2000-10-27 | 2006-08-01 | The Board Of Regents Of The University Of Texas System | Isolation of binding proteins with high affinity to ligands |
US6576468B1 (en) | 2000-11-07 | 2003-06-10 | Morphotek, Inc. | Methods for obtaining microbe-resistant mammalian cells from hypermutable mammalian cells |
US6808894B1 (en) | 2000-11-07 | 2004-10-26 | Morphotek, Inc. | Methods for generating genetically altered antibody producing cell lines with improved antibody characteristics |
US7235643B2 (en) * | 2000-11-07 | 2007-06-26 | Morphotek, Inc. | Antibodies and methods for generating genetically altered antibodies with high affinity |
US7223598B2 (en) * | 2000-11-07 | 2007-05-29 | Morphotek, Inc. | Methods for isolating novel antimicrobial agents from hypermutable mammalian cells |
US6737268B1 (en) | 2000-11-14 | 2004-05-18 | Morphotek, Inc. | Method for generating genetically altered antigens |
US6958213B2 (en) | 2000-12-12 | 2005-10-25 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function |
US20020086292A1 (en) | 2000-12-22 | 2002-07-04 | Shigeaki Harayama | Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules |
US6982169B2 (en) | 2001-01-15 | 2006-01-03 | Morphotek, Inc. | Chemical inhibitors of mismatch repair |
ATE501251T1 (en) | 2001-01-25 | 2011-03-15 | Evolva Ltd | CELL LIBRARY |
US8008459B2 (en) | 2001-01-25 | 2011-08-30 | Evolva Sa | Concatemers of differentially expressed multiple genes |
AU2002255907A1 (en) * | 2001-03-23 | 2002-10-08 | William M. Muir | Incorporation of competitive effects in breeding program to increase performance levels and improve animal well being |
WO2002083868A2 (en) | 2001-04-16 | 2002-10-24 | California Institute Of Technology | Peroxide-driven cytochrome p450 oxygenase variants |
WO2003008563A2 (en) | 2001-07-20 | 2003-01-30 | California Institute Of Technology | Improved cytochrome p450 oxygenases |
US20030068808A1 (en) * | 2001-07-25 | 2003-04-10 | Nicolaides Nicholas C. | Methods for generating antibiotic resistant microbes and novel antibiotics |
WO2003012126A2 (en) * | 2001-08-03 | 2003-02-13 | Diversa Corporation | Epoxide hydrolases, nucleic acids encoding them and methods for making and using them |
US6943001B2 (en) * | 2001-08-03 | 2005-09-13 | Diversa Corporation | Epoxide hydrolases, nucleic acids encoding them and methods for making and using them |
US7758854B2 (en) * | 2001-08-30 | 2010-07-20 | Wisconsin Alumni Research Foundation | Anti-microbial agents |
US7323618B2 (en) * | 2002-02-01 | 2008-01-29 | Origen Therapeutics, Inc. | Tissue specific expression of exogenous proteins in transgenic chickens |
AU2003217716A1 (en) * | 2002-02-25 | 2003-09-09 | Cabot Corporation | Custom ligand design for biomolecular filtration and purification for bioseperation |
JP5319865B2 (en) | 2002-03-01 | 2013-10-16 | コデクシス メイフラワー ホールディングス, エルエルシー | Methods, systems, and software for identifying functional biomolecules |
WO2003078583A2 (en) | 2002-03-09 | 2003-09-25 | Maxygen, Inc. | Optimization of crossover points for directed evolution |
CN1659276A (en) | 2002-04-19 | 2005-08-24 | 戴弗萨公司 | Phospholipases, nucleic acids encoding them and methods for making and using them |
US7226771B2 (en) | 2002-04-19 | 2007-06-05 | Diversa Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
IL164687A0 (en) | 2002-05-02 | 2005-12-18 | Univ North Carolina | Cellular libraries and methods for the preparationthereof |
WO2003097834A2 (en) | 2002-05-17 | 2003-11-27 | Alligator Bioscience Ab | A method for in vitro molecular evolution of protein function |
US9321832B2 (en) | 2002-06-28 | 2016-04-26 | Domantis Limited | Ligand |
EP1551988B1 (en) * | 2002-07-19 | 2010-11-24 | Morphotek, Inc. | Methods for generating enhanced antibody-producing cell lines with improved growth characteristics |
DK1529110T3 (en) * | 2002-08-01 | 2012-01-30 | Evolva Ltd | Method for mixing large numbers of heterologous genes |
AU2003304275A1 (en) | 2002-08-06 | 2005-01-21 | Pioneer Hi-Bred International, Inc. | Ap1 amine oxidase variants |
AU2003303288B2 (en) * | 2002-08-16 | 2006-12-07 | Microchips, Inc. | Controlled release device and method |
US7252942B2 (en) * | 2002-12-19 | 2007-08-07 | E.I. Du Pont De Nemours And Company | Parallel chromosomal stacking of traits in bacteria |
DK2194133T3 (en) | 2003-03-06 | 2016-02-29 | Basf Enzymes Llc | Amylases, nucleic acids encoding them, and methods of making and using the same |
CA2515583C (en) | 2003-03-07 | 2015-07-14 | Diversa Corporation | Hydrolases, nucleic acids encoding them and methods for making and using them |
ES2545639T3 (en) | 2003-04-04 | 2015-09-14 | Basf Enzymes Llc | Pectate liases, nucleic acids that encode them and methods for their preparation and use |
EP1620557A2 (en) | 2003-04-29 | 2006-02-01 | Pioneer Hi-Bred International, Inc. | Novel glyphosate-n-acetyltransferase (gat) genes |
JP2006526990A (en) * | 2003-06-06 | 2006-11-30 | アーバージェン リミテッド ライアビリティ カンパニー | Compositions and methods for modulating plant cell polysaccharides |
EP1639091B1 (en) | 2003-06-17 | 2012-12-05 | California University Of Technology | Regio- and enantioselective alkane hydroxylation with modified cytochrome p450 |
EP2404931A1 (en) | 2003-07-02 | 2012-01-11 | Verenium Corporation | Glucanases, nucleic acids encoding them and methods for making and using them |
US7435570B2 (en) | 2003-08-11 | 2008-10-14 | California Institute Of Technology | Thermostable peroxide-driven cytochrome P450 oxygenase variants and methods of use |
CA2535526C (en) | 2003-08-11 | 2015-09-29 | Diversa Corporation | Laccases, nucleic acids encoding them and methods for making and using them |
US7516086B2 (en) | 2003-09-24 | 2009-04-07 | Idearc Media Corp. | Business rating placement heuristic |
US7050990B1 (en) | 2003-09-24 | 2006-05-23 | Verizon Directories Corp. | Information distribution system |
EP1542074A1 (en) * | 2003-12-11 | 2005-06-15 | Heptagon OY | Manufacturing a replication tool, sub-master or replica |
WO2005081632A2 (en) * | 2004-02-26 | 2005-09-09 | State Of Israel, Ministry Of Agriculture, Agricultural Research Organization | Enzymes, cells and methods for site specific recombination at asymmetric sites |
US8055553B1 (en) | 2006-01-19 | 2011-11-08 | Verizon Laboratories Inc. | Dynamic comparison text functionality |
US20110218118A1 (en) * | 2004-06-03 | 2011-09-08 | Phylogica Limited | Peptide modulators of cellular phenotype and bi-nucleic acid fragment library |
ES2373397T3 (en) | 2004-06-08 | 2012-02-03 | Microbiogen Pty Ltd | NON-RECOMBINANT SACCHAROMYCES STRAINS THAT GROW IN XILOSA. |
ES2461857T3 (en) | 2004-06-16 | 2014-05-21 | Dsm Ip Assets B.V. | Compositions and methods for enzymatic discoloration of chlorophyll |
US20060003454A1 (en) * | 2004-07-02 | 2006-01-05 | Conjugon, Inc. | Non-dividing donor cells for gene transfer |
US7799906B1 (en) | 2004-09-22 | 2010-09-21 | Arborgen, Llc | Compositions and methods for modulating lignin of a plant |
US7485423B2 (en) | 2004-11-11 | 2009-02-03 | Modular Genetics, Inc. | Ladder assembly and system for generating diversity |
US20060105375A1 (en) * | 2004-11-16 | 2006-05-18 | Bachmann Brian O | Bioretrosynthetic methods for preparing products and compositions related thereto |
JP2006246792A (en) * | 2005-03-10 | 2006-09-21 | Bussan Nanotech Research Institute Inc | Method for detecting microorganism and bacteriolytic reagent |
EP1869174B1 (en) | 2005-03-10 | 2015-02-18 | BASF Enzymes LLC | Lyase enzymes, nucleic acids encoding them and methods for making and using them |
KR101393679B1 (en) | 2005-03-15 | 2014-05-21 | 비피 코포레이션 노쓰 아메리카 인코포레이티드 | Cellulases, nucleic acids encoding them and methods for making and using them |
US8715988B2 (en) | 2005-03-28 | 2014-05-06 | California Institute Of Technology | Alkane oxidation by modified hydroxylases |
US11214817B2 (en) | 2005-03-28 | 2022-01-04 | California Institute Of Technology | Alkane oxidation by modified hydroxylases |
US20060270040A1 (en) * | 2005-05-26 | 2006-11-30 | Conjugon, Inc. | Compositions and methods for treating tissue |
GB2432366B (en) * | 2005-11-19 | 2007-11-21 | Alligator Bioscience Ab | A method for in vitro molecular evolution of protein function |
EP1987142A4 (en) | 2006-02-02 | 2009-07-15 | Verenium Corp | Esterases and related nucleic acids and methods |
EP2415864B1 (en) | 2006-02-10 | 2014-02-12 | Verenium Corporation | Oligomerase-2 (or beta-xylosidase) enzymes, nucleic acids encoding them and methods for making and using them |
TR201809912T4 (en) | 2006-02-14 | 2018-07-23 | Bp Corp North America Inc | Xylanases, nucleic acids encoding them, and methods for making and using them. |
CA2645225A1 (en) | 2006-03-07 | 2007-09-13 | Verenium Corporation | Aldolases, nucleic acids encoding them and methods for making and using them |
EP2112227B1 (en) | 2006-03-07 | 2013-05-15 | Cargill, Incorporated | Aldolases, nucleic acids encoding them and methods for making and using them |
BRPI0715442B1 (en) | 2006-07-21 | 2018-01-30 | Xyleco, Inc. | METHOD FOR PRODUCING PRODUCTS FROM BIOMASS CELLULOSTIC OR LIGNOCELLULOSTIC MATERIAL |
EP2069389B1 (en) | 2006-08-04 | 2014-10-08 | BP Corporation North America Inc. | Glucanases, nucleic acids encoding them and methods for making and using them |
US8026085B2 (en) | 2006-08-04 | 2011-09-27 | California Institute Of Technology | Methods and systems for selective fluorination of organic molecules |
US8252559B2 (en) | 2006-08-04 | 2012-08-28 | The California Institute Of Technology | Methods and systems for selective fluorination of organic molecules |
CA2663001A1 (en) | 2006-09-21 | 2008-03-27 | Verenium Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
CA2663819C (en) | 2006-09-21 | 2019-04-30 | Verenium Corporation | Phytases, nucleic acids encoding them and methods for making and using them |
ES2620288T3 (en) | 2006-12-21 | 2017-06-28 | Basf Enzymes Llc | Amylases and glucoamylases, nucleic acids that encode them and methods to form and use them |
CA2674721C (en) | 2007-01-30 | 2018-04-03 | Verenium Corporation | Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them |
US20090004715A1 (en) | 2007-06-01 | 2009-01-01 | Solazyme, Inc. | Glycerol Feedstock Utilization for Oil-Based Fuel Manufacturing |
USRE46733E1 (en) | 2007-10-03 | 2018-02-27 | Bp Corporation North America Inc. | Xylanases, nucleic acids encoding them and methods for making and using them |
FR2923491B1 (en) | 2007-11-14 | 2012-12-14 | Deinove Sa | USE OF BACTERIA FOR THE PRODUCTION OF BIOENERGY SOURCES |
WO2009068604A1 (en) | 2007-11-27 | 2009-06-04 | Vib Vzw | Marker assisted directed evolution |
WO2009088753A1 (en) | 2008-01-03 | 2009-07-16 | Verenium Corporation | Isomerases, nucleic acids encoding them and methods for making and using them |
DK2238242T5 (en) | 2008-01-03 | 2015-08-03 | Basf Enzymes Llc | -Transferases and oxidoreductases, nucleic acids encoding FOR THEM, AND PROCESSES FOR THE STILL LING AND USE THEREOF |
WO2009143397A2 (en) | 2008-05-23 | 2009-11-26 | E. I. Du Pont De Nemours And Company | Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants |
US8198062B2 (en) | 2008-08-29 | 2012-06-12 | Dsm Ip Assets B.V. | Hydrolases, nucleic acids encoding them and methods for making and using them |
US8357503B2 (en) | 2008-08-29 | 2013-01-22 | Bunge Oils, Inc. | Hydrolases, nucleic acids encoding them and methods for making and using them |
US8153391B2 (en) | 2008-08-29 | 2012-04-10 | Bunge Oils, Inc. | Hydrolases, nucleic acids encoding them and methods for making and using them |
MX2011003039A (en) | 2008-09-26 | 2011-08-15 | Tocagen Inc | Gene therapy vectors and cytosine deaminases. |
NZ596459A (en) | 2009-05-21 | 2013-11-29 | Verenium Corp | Phytases, nucleic acids encoding them and methods for making and using them |
DK3301208T3 (en) | 2009-09-18 | 2020-12-14 | Codexis Inc | REDUCED CODON MUTAGENESIS |
UA109884C2 (en) | 2009-10-16 | 2015-10-26 | A POLYPEPTIDE THAT HAS THE ACTIVITY OF THE PHOSPHATIDYLINOSYTOL-SPECIFIC PHOSPHOLIPASE C, NUCLEIC ACID, AND METHOD OF METHOD | |
UA111708C2 (en) | 2009-10-16 | 2016-06-10 | Бандж Ойлз, Інк. | METHOD OF OIL REFINING |
CA2798937A1 (en) | 2010-05-28 | 2011-12-01 | Codexis, Inc. | Pentose fermentation by a recombinant microorganism |
EP2603591A1 (en) | 2010-08-13 | 2013-06-19 | Pioneer Hi-Bred International Inc. | Compositions and methods comprising sequences having hydroxyphenylpyruvate dioxygenase (hppd) activity |
US9322027B2 (en) | 2010-08-20 | 2016-04-26 | Shell Oil Company | Expression constructs comprising fungal promoters |
CN103080306B (en) | 2010-08-20 | 2015-02-18 | 科德克希思公司 | Use of glycoside hydrolase 61 family proteins in processing of cellulose |
WO2012051055A2 (en) | 2010-10-06 | 2012-04-19 | Bp Corporation North America Inc. | Variant cbh i polypeptides |
CN102199624B (en) * | 2011-03-24 | 2013-01-23 | 北京济福霖生物技术有限公司 | Method for efficiently producing recombinant proteins in mammary glands by utilizing artificial chromosomes |
EP2691517A4 (en) | 2011-03-30 | 2015-04-22 | Codexis Inc | Pentose fermentation by a recombinant microorganism |
BR112013033614A2 (en) | 2011-06-30 | 2018-05-29 | Codexis Inc | pentose fermentation by recombinant microorganism |
US9322007B2 (en) | 2011-07-22 | 2016-04-26 | The California Institute Of Technology | Stable fungal Cel6 enzyme variants |
DK2748317T3 (en) | 2011-08-22 | 2017-07-17 | Codexis Inc | GH61 glycoside hydrolase protein variants and cofactors that enhance GH61 activity |
EP2760997A4 (en) | 2011-09-30 | 2015-02-11 | Codexis Inc | Fungal proteases |
WO2013096244A1 (en) | 2011-12-20 | 2013-06-27 | Codexis, Inc. | Endoglucanase 1b (eg1b) variants |
BR112014027468A2 (en) | 2012-05-04 | 2017-06-27 | Du Pont | isolated or recombinant polynucleotide, recombinant DNA construction, cell, plant, plant explant, transgenic seed, isolated polypeptide, composition, methods of producing meganuclease, introducing disruption and integrating a polynucleotide. |
DK2859099T3 (en) | 2012-06-11 | 2019-07-29 | Codexis Inc | MUSHROOMS-BETA-xylosidase VERSIONS |
WO2014060973A1 (en) | 2012-10-19 | 2014-04-24 | Indian Oil Corporation Limited | Improved microalgae strains and use thereof |
EP2912169A4 (en) | 2012-10-29 | 2016-11-23 | Univ Rochester | Artemisinin derivatives, methods for their preparation and their use as antimalarial agents |
EP2922953A4 (en) | 2012-11-20 | 2016-11-09 | Shell Int Research | Pentose fermentation by a recombinant microorganism |
WO2014083534A1 (en) | 2012-11-29 | 2014-06-05 | Indian Oil Corporation Limited | Mixotrophic cultivation of microalgae for the production of biofuel |
GB201308828D0 (en) | 2013-03-12 | 2013-07-03 | Verenium Corp | Phytase |
GB201308843D0 (en) | 2013-03-14 | 2013-07-03 | Verenium Corp | Phytase formulation |
US20160040149A1 (en) | 2013-03-14 | 2016-02-11 | Pioneer Hi-Bred International Inc. | Compositions Having Dicamba Decarboxylase Activity and Methods of Use |
US20160053277A1 (en) | 2013-03-14 | 2016-02-25 | Pioneer Hi-Bred International, Inc. | Compositions Having Dicamba Decarboxylase Activity and Methods of Use |
CN105473605A (en) | 2013-03-15 | 2016-04-06 | 先锋国际良种公司 | Phi-4 polypeptides and methods for their use |
CA2909719C (en) | 2013-04-18 | 2022-09-27 | Codexis, Inc. | Engineered phenylalanine ammonia lyase polypeptides |
SG11201600059QA (en) * | 2013-07-09 | 2016-02-26 | Harvard College | Multiplex rna-guided genome engineering |
AR095173A1 (en) | 2013-07-25 | 2015-09-30 | Basf Enzymes Llc | FITASA |
EP3032942B1 (en) | 2013-08-16 | 2020-03-11 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
US10667524B2 (en) | 2013-09-13 | 2020-06-02 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
WO2015048572A1 (en) | 2013-09-27 | 2015-04-02 | Codexis, Inc. | Automated screening of enzyme variants |
WO2015073555A1 (en) | 2013-11-13 | 2015-05-21 | Codexis, Inc. | Engineered imine reductases and methods for the reductive amination of ketone and amine compounds |
US10544191B2 (en) | 2013-12-23 | 2020-01-28 | University Of Rochester | Methods and compositions for ribosomal synthesis of macrocyclic peptides |
BR112016018287A2 (en) | 2014-02-07 | 2017-10-10 | Du Pont | insecticide proteins and methods for their use |
CN114763376A (en) | 2014-02-07 | 2022-07-19 | 先锋国际良种公司 | Insecticidal proteins and methods of use thereof |
US9605252B2 (en) | 2014-04-16 | 2017-03-28 | Codexis, Inc. | Engineered tyrosine ammonia lyase |
DK3167052T3 (en) | 2014-07-09 | 2020-02-10 | Codexis Inc | P450-BM3 VARIATIONS WITH IMPROVED ACTIVITY |
CN113372421A (en) | 2014-10-16 | 2021-09-10 | 先锋国际良种公司 | Insecticidal proteins and methods of use thereof |
DK3224354T3 (en) | 2014-11-25 | 2021-03-01 | Codexis Inc | MANIPULATED IMINREDUCTASES AND PROCEDURES FOR THE REDUCTIVE AMINATION OF KETONE AND AMIN COMPOUNDS |
HUE062016T2 (en) | 2014-12-22 | 2023-09-28 | Codexis Inc | Human alpha-galactosidase variants |
US10696953B2 (en) | 2015-02-10 | 2020-06-30 | Codexis, Inc. | Ketoreductase polypeptides for the synthesis of chiral compounds |
BR112017019419B1 (en) | 2015-03-11 | 2022-10-11 | E. I. Du Pont De Nemours And Company | DNA CONSTRUCTION, METHOD OF OBTAINING A TRANSGENIC PLANT AND METHOD TO CONTROL A CORN ROOT Worm PEST POPULATION |
MX2017014213A (en) | 2015-05-07 | 2018-03-28 | Codexis Inc | Penicillin-g acylases. |
WO2016186986A1 (en) | 2015-05-19 | 2016-11-24 | Pioneer Hi Bred International Inc | Insecticidal proteins and methods for their use |
CN108271374B (en) | 2015-07-07 | 2021-09-21 | 科德克希思公司 | Novel P450-BM3 variants with improved activity |
US11198709B2 (en) | 2015-08-06 | 2021-12-14 | E. I. Du Pont De Nemours And Company | Plant derived insecticidal proteins and methods for their use |
WO2017090055A1 (en) * | 2015-11-25 | 2017-06-01 | Surana Rajendra | A method of producing high amount of ethanol at high temperature by modified yeast strain saccharomyces cerevisiae |
US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
WO2017100376A2 (en) | 2015-12-07 | 2017-06-15 | Zymergen, Inc. | Promoters from corynebacterium glutamicum |
US11208649B2 (en) | 2015-12-07 | 2021-12-28 | Zymergen Inc. | HTP genomic engineering platform |
CA3007840C (en) * | 2015-12-07 | 2020-09-15 | Zymergen Inc. | Microbial strain improvement by a htp genomic engineering platform |
WO2017105987A1 (en) | 2015-12-18 | 2017-06-22 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
EA201892293A1 (en) | 2016-05-04 | 2019-04-30 | Пайонир Хай-Бред Интернэшнл, Инк. | INSECTICIDAL PROTEINS AND METHODS OF THEIR APPLICATION |
US10724025B2 (en) | 2016-05-05 | 2020-07-28 | Codexis, Inc. | Penicillin-G acylases |
WO2017213758A1 (en) | 2016-06-09 | 2017-12-14 | Codexis, Inc. | Biocatalysts and methods for hydroxylation of chemical compounds |
BR112018075932A2 (en) | 2016-06-15 | 2019-10-01 | Codexis Inc | unnaturally occurring beta-glycosidase variant, recombinant polynucleotide, vector, host cell, methods for producing at least one unnaturally occurring beta-glucosidase variant for conversion of a glycosyl group-acceptor substrate to a beta-glycosylated product and for transglycosylation of a substrate, and, composition. |
EP3472186A1 (en) | 2016-06-17 | 2019-04-24 | National Hellenic Research Foundation | Systems for recombinant protein production |
EP3478845A4 (en) | 2016-06-30 | 2019-07-31 | Zymergen, Inc. | Methods for generating a glucose permease library and uses thereof |
JP2019519242A (en) | 2016-06-30 | 2019-07-11 | ザイマージェン インコーポレイテッド | Method for generating a bacterial hemoglobin library and its use |
EP3478052B1 (en) | 2016-07-01 | 2021-08-25 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
EP3515926B1 (en) | 2016-08-26 | 2023-10-25 | Codexis, Inc. | Engineered imine reductases and methods for the reductive amination of ketone and amine compounds |
US11021716B2 (en) | 2016-11-01 | 2021-06-01 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
CN110088123B (en) | 2016-12-14 | 2023-10-20 | 先锋国际良种公司 | Insecticidal proteins and methods of use thereof |
CA3046226A1 (en) | 2016-12-22 | 2018-06-28 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
KR20190102063A (en) | 2017-01-05 | 2019-09-02 | 코덱시스, 인코포레이티드 | Penicillin G acylase |
TW202237834A (en) | 2017-02-03 | 2022-10-01 | 美商克迪科思股份有限公司 | Engineered glycosyltransferases and steviol glycoside glucosylation methods |
US20190390219A1 (en) | 2017-02-08 | 2019-12-26 | Pioneer Hi-Bred International, Inc. | Insecticidal combinations of plant derived insecticidal proteins and methods for their use |
JP2020505942A (en) | 2017-02-13 | 2020-02-27 | コデクシス, インコーポレイテッド | Phenylalanine ammonia lyase polypeptide |
US11021729B2 (en) | 2017-04-27 | 2021-06-01 | Codexis, Inc. | Ketoreductase polypeptides and polynucleotides |
CA3062550A1 (en) | 2017-05-08 | 2018-11-15 | Codexis, Inc. | Engineered ligase variants |
US11555203B2 (en) | 2017-05-11 | 2023-01-17 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
EP3635110A2 (en) * | 2017-06-06 | 2020-04-15 | Zymergen, Inc. | A high-throughput (htp) genomic engineering platform for improving saccharopolyspora spinosa |
CN110945125A (en) * | 2017-06-06 | 2020-03-31 | 齐默尔根公司 | HTP genetic engineering modification platform for improving escherichia coli |
EP3638688A4 (en) | 2017-06-14 | 2021-07-07 | Codexis, Inc. | Engineered transaminase polypeptides for industrial biocatalysis |
CN111051506B (en) | 2017-06-27 | 2023-11-24 | 科德克希思公司 | Penicillin G acylase |
CA3066767A1 (en) | 2017-06-30 | 2019-01-03 | Codexis, Inc. | T7 rna polymerase variants |
CN111032863A (en) | 2017-06-30 | 2020-04-17 | 科德克希思公司 | T7RNA polymerase variants |
WO2019036202A1 (en) * | 2017-08-17 | 2019-02-21 | Cargill, Incorporated | Genetically modified haploid issatchenkia orientalis |
WO2019040335A1 (en) | 2017-08-19 | 2019-02-28 | University Of Rochester | Micheliolide derivatives, methods for their preparation and their use as anticancer and antiinflammatory agents |
ES2704201B2 (en) * | 2017-09-14 | 2019-11-18 | Tomsa Destil S L | Saccharomyces cerevisiae strain and its use for the elaboration of alcoholic products |
EP3706780A4 (en) | 2017-11-07 | 2021-12-22 | Codexis, Inc. | Transglutaminase variants |
MX2020006222A (en) | 2017-12-13 | 2020-08-31 | Codexis Inc | Carboxyesterase polypeptides for amide coupling. |
EP3794116A2 (en) | 2018-05-17 | 2021-03-24 | BP Corporation North America Inc. | Production of 2-keto-3-deoxy-d-gluconic acid in filamentous fungi |
WO2019241132A1 (en) | 2018-06-12 | 2019-12-19 | Codexis, Inc. | Engineered tyrosine ammonia lyase |
SG11202012107RA (en) | 2018-07-09 | 2021-01-28 | Codexis Inc | Engineered deoxyribose-phosphate aldolases |
MX2021000328A (en) | 2018-07-09 | 2021-03-25 | Codexis Inc | Engineered galactose oxidase variant enzymes. |
MX2021000322A (en) | 2018-07-09 | 2021-03-25 | Codexis Inc | Engineered phosphopentomutase variant enzymes. |
SG11202012111UA (en) | 2018-07-09 | 2021-01-28 | Codexis Inc | Engineered purine nucleoside phosphorylase variant enzymes |
CN112673091A (en) | 2018-07-09 | 2021-04-16 | 科德克希思公司 | Engineered pantothenate kinase variant enzymes |
CN112672989A (en) | 2018-07-12 | 2021-04-16 | 科德克希思公司 | Engineered phenylalanine ammonia lyase polypeptides |
KR20210040408A (en) | 2018-07-30 | 2021-04-13 | 코덱시스, 인코포레이티드 | Engineered glycosyltransferase and steviol glycoside glucosylation method |
AU2019334672A1 (en) | 2018-09-07 | 2021-03-25 | Basf Plant Science Company Gmbh | Improved method for the production of high levels of PUFA in plants |
US20230183728A1 (en) | 2018-09-07 | 2023-06-15 | Basf Plant Science Company Gmbh | Improved method for the production of high levels of pufa in plants |
EP3847263A1 (en) | 2018-09-07 | 2021-07-14 | Basf Plant Science Company GmbH | Improved method for the production of high levels of pufa in plants |
WO2020092216A1 (en) | 2018-10-29 | 2020-05-07 | Codexis, Inc. | Engineered dna polymerase variants |
AU2019397401A1 (en) | 2018-12-14 | 2021-06-17 | Codexis, Inc. | Engineered tyrosine ammonia lyase |
CA3123598A1 (en) | 2018-12-20 | 2020-06-25 | Codexis, Inc. | Human alpha-galactosidase variants |
BR112022011895A2 (en) | 2019-12-20 | 2022-09-06 | Basf Se | DECREASE IN TERPENE TOXIDITY AND INCREASED POTENTIAL PRODUCTION IN MICROORGANISMS |
CN115427557A (en) | 2020-04-10 | 2022-12-02 | 科德克希思公司 | Engineered transaminase polypeptides |
AU2021285040A1 (en) | 2020-06-04 | 2023-01-05 | Isobionics B.V. | Synthetic santalene synthases |
CN111513024A (en) * | 2020-06-19 | 2020-08-11 | 宁夏绿方水产良种繁育有限公司 | Soft-shelled turtle breeding method capable of increasing survival rate |
BR112022027035A2 (en) | 2020-07-14 | 2023-04-11 | Pioneer Hi Bred Int | INSECTICIDAL PROTEINS AND METHODS FOR THE USE OF THEM |
EP4204552A1 (en) | 2020-08-28 | 2023-07-05 | Codexis, Inc. | Engineered amylase variants |
EP4015626A1 (en) | 2020-12-18 | 2022-06-22 | Isobionics B.V. | Enzymes and methods for fermentative production of monoterpene esters |
EP4262804A2 (en) | 2020-12-18 | 2023-10-25 | Codexis, Inc. | Engineered uridine phosphorylase variant enzymes |
WO2022212824A1 (en) | 2021-04-02 | 2022-10-06 | Codexis, Inc. | Engineered adenylate kinase variant enzymes |
CA3214975A1 (en) | 2021-04-02 | 2022-10-06 | Codexis, Inc. | Engineered cyclic gmp-amp synthase (cgas) variant enzymes |
IL305928A (en) | 2021-04-02 | 2023-11-01 | Codexis Inc | Engineered acetate kinase variant enzymes |
CA3214973A1 (en) | 2021-04-02 | 2022-10-06 | Codexis, Inc. | Engineered guanylate kinase variant enzymes |
CN117795087A (en) | 2021-08-02 | 2024-03-29 | 巴斯夫欧洲公司 | Novel production of aromatic compounds with violet subunit ethane synthase |
WO2023173084A1 (en) | 2022-03-11 | 2023-09-14 | University Of Rochester | Cyclopeptibodies and uses thereof |
WO2024040020A1 (en) | 2022-08-15 | 2024-02-22 | Absci Corporation | Quantitative affinity activity specific cell enrichment |
Family Cites Families (111)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS54163880A (en) | 1978-04-26 | 1979-12-26 | Glaxo Group Ltd | Improvement in cephalosporin production |
CA1220733A (en) * | 1982-12-27 | 1987-04-21 | Shigeru Takahashi | Method for promoting fusion of plant protoplast |
HU194306B (en) | 1983-05-16 | 1988-01-28 | Gyogyszerkutato Intezet | Process for influencing biosynthesis of antibiotics with in vivo genetical recombination |
US4940834A (en) | 1983-12-05 | 1990-07-10 | Lofts, Inc. | Plants having endophytio-fungus-enhanced performance and method of producing same |
DE3588239T3 (en) | 1985-03-30 | 2007-03-08 | Kauffman, Stuart A., Santa Fe | A method for obtaining DNA, RNA, peptides, polypeptides or proteins by DMS recombinant methods |
US6492107B1 (en) | 1986-11-20 | 2002-12-10 | Stuart Kauffman | Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique |
US5866363A (en) | 1985-08-28 | 1999-02-02 | Pieczenik; George | Method and means for sorting and identifying biological information |
US5824469A (en) | 1986-07-17 | 1998-10-20 | University Of Washington | Method for producing novel DNA sequences with biological activity |
US4940839A (en) * | 1986-10-01 | 1990-07-10 | Dna Plant Technology Corporation | Method of tomato protoplast fusion and regeneration of hybrid plants therefrom |
US4889806A (en) | 1987-04-15 | 1989-12-26 | Washington University | Large DNA cloning system based on yeast artificial chromosomes |
DE3889546T2 (en) | 1987-12-21 | 1994-09-08 | Univ Toledo | TRANSFORMATION OF Germinating PLANT SEEDS WITH THE HELP OF AGROBACTERIUM. |
FR2641793B1 (en) | 1988-12-26 | 1993-10-01 | Setratech | METHOD OF IN VIVO RECOMBINATION OF DNA SEQUENCES HAVING BASIC MATCHING |
DD282028A5 (en) | 1989-02-16 | 1990-08-29 | Akad Wissenschaften Ddr | PROCESS FOR PREPARING THERMOSTABILES, HYBRIDEN BACILLUS BETA-1,3-1,4-GLUCANASE |
JP2855130B2 (en) | 1989-07-19 | 1999-02-10 | 吉富製薬株式会社 | Fusion yeast for expressing heterologous protein, method for producing the same, and method for producing heterologous protein |
AU633958B2 (en) | 1989-07-25 | 1993-02-11 | Cell Genesys, Inc. | Homologous recombination for universal donor cells and chimeric mammalian hosts |
US5574205A (en) | 1989-07-25 | 1996-11-12 | Cell Genesys | Homologous recombination for universal donor cells and chimeric mammalian hosts |
US5426040A (en) * | 1989-08-17 | 1995-06-20 | Northeastern University | Methods for producing improved strains of seaweed by fusion of spore-protoplasts, and resultant seaweeds and phycocolloids |
ES2164634T3 (en) | 1989-10-18 | 2002-03-01 | Us Health | PRODUCTION AND USE OF HUMAN NM23-H2 PROTEIN AND ANTIBODIES INTENDED TO SUCH EFFECT. |
KR100233781B1 (en) | 1989-11-06 | 1999-12-01 | 스테펜 에이. 쉐어윈 | Production of proteins using homologous recombination |
US5877402A (en) | 1990-05-01 | 1999-03-02 | Rutgers, The State University Of New Jersey | DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein |
WO1992003917A1 (en) | 1990-08-29 | 1992-03-19 | Genpharm International | Homologous recombination in mammalian cells |
WO1992017598A1 (en) * | 1991-03-29 | 1992-10-15 | The Board Of Trustees Of The University Of Illinois | Production fo transgenic soybean plants |
US5512463A (en) | 1991-04-26 | 1996-04-30 | Eli Lilly And Company | Enzymatic inverse polymerase chain reaction library mutagenesis |
US5514568A (en) | 1991-04-26 | 1996-05-07 | Eli Lilly And Company | Enzymatic inverse polymerase chain reaction |
US6071889A (en) | 1991-07-08 | 2000-06-06 | Neurospheres Holdings Ltd. | In vivo genetic modification of growth factor-responsive neural precursor cells |
US5843643A (en) | 1992-02-21 | 1998-12-01 | Ratner; Paul L. | Site-specific transfection of eukaryotic cells using polypeptide-linked recombinant nucleic acid |
WO1993019170A1 (en) | 1992-03-16 | 1993-09-30 | Jacob Nathaniel Wohlstadter | Selection methods |
US5763240A (en) | 1992-04-24 | 1998-06-09 | Sri International | In vivo homologous sequence targeting in eukaryotic cells |
JPH07506252A (en) | 1992-04-24 | 1995-07-13 | エス・アール・アイ・インターナシヨナル | In vivo homologous sequence targeting in eukaryotic cells |
DE4319496A1 (en) | 1992-06-17 | 1994-04-07 | Sandoz Ag | Mushrooms for the reduction of the resin content and their extraction |
WO1995003400A1 (en) | 1993-07-23 | 1995-02-02 | Johns Hopkins University School Of Medicine | Recombinationally targeted cloning in yeast artificial chromosomes |
US5643745A (en) | 1994-02-03 | 1997-07-01 | University Of Hawaii | Heterologous dimeric proteins produced in heterokaryons |
US5683899A (en) | 1994-02-03 | 1997-11-04 | University Of Hawaii | Methods and compositions for combinatorial-based discovery of new multimeric molecules |
US5928905A (en) | 1995-04-18 | 1999-07-27 | Glaxo Group Limited | End-complementary polymerase reaction |
US5837458A (en) | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
US6117679A (en) | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US5834252A (en) | 1995-04-18 | 1998-11-10 | Glaxo Group Limited | End-complementary polymerase reaction |
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
US6335160B1 (en) | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
US6165793A (en) | 1996-03-25 | 2000-12-26 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US5521077A (en) | 1994-04-28 | 1996-05-28 | The Leland Stanford Junior University | Method of generating multiple protein variants and populations of protein variants prepared thereby |
US5605820A (en) | 1994-06-23 | 1997-02-25 | Chemgenics Pharmaceuticals, Inc. | Production of strains having biological activities by sexual crosses between vegetatively incompatible strains of Aspergillus nidulans |
US5514588A (en) | 1994-12-13 | 1996-05-07 | Exxon Research And Engineering Company | Surfactant-nutrients for bioremediation of hydrocarbon contaminated soils and water |
AU723619B2 (en) | 1995-04-24 | 2000-08-31 | Chromaxome Corporation | Methods for generating and screening novel metabolic pathways |
US6004788A (en) | 1995-07-18 | 1999-12-21 | Diversa Corporation | Enzyme kits and libraries |
US6057103A (en) | 1995-07-18 | 2000-05-02 | Diversa Corporation | Screening for novel bioactivities |
US5958672A (en) | 1995-07-18 | 1999-09-28 | Diversa Corporation | Protein activity screening of clones having DNA from uncultivated microorganisms |
US6030779A (en) | 1995-07-18 | 2000-02-29 | Diversa Corporation | Screening for novel bioactivities |
US6168919B1 (en) | 1996-07-17 | 2001-01-02 | Diversa Corporation | Screening methods for enzymes and enzyme kits |
DE69620766T2 (en) | 1995-08-11 | 2004-11-18 | Novozymes A/S | METHOD FOR THE PRODUCTION OF POLYPEPTIDE COMBS |
US5962258A (en) | 1995-08-23 | 1999-10-05 | Diversa Corporation | Carboxymethyl cellulase fromthermotoga maritima |
US6051409A (en) | 1995-09-25 | 2000-04-18 | Novartis Finance Corporation | Method for achieving integration of exogenous DNA delivered by non-biological means to plant cells |
US5965408A (en) | 1996-07-09 | 1999-10-12 | Diversa Corporation | Method of DNA reassembly by interrupting synthesis |
US20030215798A1 (en) | 1997-06-16 | 2003-11-20 | Diversa Corporation | High throughput fluorescence-based screening for novel enzymes |
US6489145B1 (en) | 1996-07-09 | 2002-12-03 | Diversa Corporation | Method of DNA shuffling |
US5830696A (en) | 1996-12-05 | 1998-11-03 | Diversa Corporation | Directed evolution of thermophilic enzymes |
US5962283A (en) | 1995-12-07 | 1999-10-05 | Diversa Corporation | Transminases and amnotransferases |
US5939250A (en) | 1995-12-07 | 1999-08-17 | Diversa Corporation | Production of enzymes having desired activities by mutagenesis |
US6171820B1 (en) | 1995-12-07 | 2001-01-09 | Diversa Corporation | Saturation mutagenesis in directed evolution |
US5814473A (en) | 1996-02-09 | 1998-09-29 | Diversa Corporation | Transaminases and aminotransferases |
US5773221A (en) | 1995-12-15 | 1998-06-30 | Oceanix Biosciences Corporation | Method of recovering a biological molecule from a recombinant microorganism |
EP0873398B1 (en) | 1996-01-10 | 2005-04-20 | Novozymes A/S | A method for in vivo production of a mutant library in cells |
US5942430A (en) | 1996-02-16 | 1999-08-24 | Diversa Corporation | Esterases |
US5958751A (en) | 1996-03-08 | 1999-09-28 | Diversa Corporation | α-galactosidase |
US6096548A (en) | 1996-03-25 | 2000-08-01 | Maxygen, Inc. | Method for directing evolution of a virus |
US5783431A (en) | 1996-04-24 | 1998-07-21 | Chromaxome Corporation | Methods for generating and screening novel metabolic pathways |
US5789228A (en) | 1996-05-22 | 1998-08-04 | Diversa Corporation | Endoglucanases |
US5877001A (en) | 1996-06-17 | 1999-03-02 | Diverso Corporation | Amidase |
US5763239A (en) | 1996-06-18 | 1998-06-09 | Diversa Corporation | Production and use of normalized DNA libraries |
WO1997048416A1 (en) | 1996-06-19 | 1997-12-24 | Recombinant Biocatalysis, Inc. | Thermostable phosphatases |
US5939300A (en) | 1996-07-03 | 1999-08-17 | Diversa Corporation | Catalases |
AU6487196A (en) | 1996-07-09 | 1998-02-02 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Transformation-associated recombination cloning |
US5869718A (en) | 1996-07-15 | 1999-02-09 | Julien; Jean-Pierre | Homologous recombination for animal model exhibiting reduced levels or elimination of a neuronal intermediate filament protein |
US6093873A (en) | 1996-08-19 | 2000-07-25 | Institut National De La Sante Et De La Recherche Medicale | Genetically engineered mice containing alterations in the gene encoding RXR |
US6173410B1 (en) | 1996-08-21 | 2001-01-09 | Texas Instruments Incorporated | Microprocessor circuits, systems and methods for conditioning information prefetching based on resource burden |
CA2268265A1 (en) | 1996-09-27 | 1998-04-02 | Maxygen, Inc. | Methods for optimization of gene therapy by recursive sequence shuffling and selection |
WO1998013487A1 (en) | 1996-09-27 | 1998-04-02 | Maxygen, Inc. | Methods for optimization of gene therapy by recursive sequence shuffling and selection |
ATE295850T1 (en) | 1996-12-06 | 2005-06-15 | Diversa Corp | GLYCOSIDASE ENZYMES |
WO1998028416A1 (en) | 1996-12-20 | 1998-07-02 | Novo Nordisk A/S | In vivo recombination |
US7148054B2 (en) | 1997-01-17 | 2006-12-12 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
EP1007732B1 (en) | 1997-01-17 | 2006-07-26 | Maxygen, Inc. | EVOLUTION OF procaryotic WHOLE CELLS BY RECURSIVE SEQUENCE RECOMBINATION |
US6326204B1 (en) | 1997-01-17 | 2001-12-04 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
US5929250A (en) | 1997-01-23 | 1999-07-27 | Smithkline Beecham Corporation | IL-8 receptor antagonists |
CA2281931A1 (en) | 1997-02-13 | 1998-08-20 | The Dow Chemical Company | Recombinant haloaliphatic dehalogenases |
US6387862B2 (en) | 1997-03-07 | 2002-05-14 | The Procter & Gamble Company | Bleach compositions |
DE69839668D1 (en) | 1997-03-18 | 2008-08-14 | Novozymes As | An In Vitro Method for Making a DNA Library |
US6159688A (en) | 1997-03-18 | 2000-12-12 | Novo Nordisk A/S | Methods of producing polynucleotide variants |
US6159687A (en) | 1997-03-18 | 2000-12-12 | Novo Nordisk A/S | Methods for generating recombined polynucleotides |
ATE468394T1 (en) | 1997-03-18 | 2010-06-15 | Novozymes As | MIXING OF HETEROLOGUE DNA SEQUENCES |
DE69840382D1 (en) | 1997-03-18 | 2009-02-05 | Novozymes As | METHOD FOR THE PRODUCTION OF A LIBRARY THROUGH DNA SHUFFLING |
US5948653A (en) | 1997-03-21 | 1999-09-07 | Pati; Sushma | Sequence alterations using homologous recombination |
US6153410A (en) | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
BR9804857A (en) | 1997-04-22 | 1999-08-24 | Gist Brocades Bv | Process for the fermentative production of deacylated cephalosporins |
US6051049A (en) | 1997-05-29 | 2000-04-18 | Exothermic Distribution Corporation | Utilization of strontium aluminate in steelmaking |
US5948666A (en) | 1997-08-06 | 1999-09-07 | Diversa Corporation | Isolation and identification of polymerases |
US5876997A (en) | 1997-08-13 | 1999-03-02 | Diversa Corporation | Phytase |
US6610528B1 (en) | 1997-08-26 | 2003-08-26 | Diversa Corporation | Microbial enrichment using a container having a plurality of solid support particles |
AU9374298A (en) | 1997-09-12 | 1999-04-05 | Oceanix Biosciences Corporation | Preparation and use of biofilm-degrading, multiple-specificit y, hydrolytic enzyme mixtures |
US6846627B2 (en) | 1997-10-15 | 2005-01-25 | Diversa Corporation | Screening for novel compounds which regulate biological interactions |
AU1124499A (en) | 1997-10-28 | 1999-05-17 | Maxygen, Inc. | Human papillomavirus vectors |
US6596539B1 (en) | 1997-10-31 | 2003-07-22 | Maxygen, Inc. | Modification of virus tropism and host range by viral genome shuffling |
US6232112B1 (en) | 1997-11-24 | 2001-05-15 | Flinders Technologies Pty Ltd Of Flinders University | Reagents and methods for diversification of DNA |
WO1999041368A2 (en) | 1998-02-11 | 1999-08-19 | Maxygen, Inc. | Optimization of immunomodulatory properties of genetic vaccines |
AU3289199A (en) | 1998-02-11 | 1999-08-30 | Maxygen, Inc. | Antigen library immunization |
AU744699B2 (en) | 1998-03-04 | 2002-02-28 | Verenium Corporation | Production and use of normalized DNA libraries |
CN1314911A (en) | 1998-05-01 | 2001-09-26 | 麦克西根股份有限公司 | Optimization of pest resistance genes using DNA shuffling |
AU4441699A (en) | 1998-06-17 | 2000-01-05 | Maxygen, Inc. | Method for producing polynucleotides with desired properties |
EP2253704B1 (en) | 1999-01-19 | 2015-08-19 | Codexis Mayflower Holdings, LLC | Oligonucleotide mediated nucleic acid recombination |
US6030267A (en) | 1999-02-19 | 2000-02-29 | Micron Technology, Inc. | Alignment method for field emission and plasma displays |
IL145165A0 (en) | 1999-03-09 | 2002-06-30 | Diversa Corp | End selection in directed evolution |
CA2329122A1 (en) | 1999-03-26 | 2000-10-05 | Diversa Corporation | Exonuclease-mediated nucleic acid reassembly in directed evolution |
-
1998
- 1998-07-15 US US09/116,188 patent/US6326204B1/en not_active Expired - Lifetime
-
1999
- 1999-07-15 CA CA2720311A patent/CA2720311A1/en not_active Abandoned
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- 1999-07-15 EP EP05077606A patent/EP1707641A3/en not_active Withdrawn
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- 1999-07-15 EP EP99935574A patent/EP1108058B1/en not_active Expired - Lifetime
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2009
- 2009-12-18 JP JP2009288163A patent/JP2010131014A/en not_active Withdrawn
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