CA2331329A1 - Diacylglycerol acyl transferase proteins - Google Patents

Diacylglycerol acyl transferase proteins Download PDF

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Publication number
CA2331329A1
CA2331329A1 CA002331329A CA2331329A CA2331329A1 CA 2331329 A1 CA2331329 A1 CA 2331329A1 CA 002331329 A CA002331329 A CA 002331329A CA 2331329 A CA2331329 A CA 2331329A CA 2331329 A1 CA2331329 A1 CA 2331329A1
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Prior art keywords
seq
polynucleotide
sequence
isolated
polypeptide
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Granted
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CA002331329A
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French (fr)
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CA2331329C (en
Inventor
Kathryn Dennis Lardizabal
Deborah Hawkins
Gregory A. Thompson
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Monsanto Technology LLC
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Individual
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/04—Anorexiants; Antiobesity agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/06—Antihyperlipidemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Abstract

The invention provides diacylglycerol acyl transferase (DAGAT) proteins, wherein said proteins are active in the formation of triacylglycerol from fatty acyl and diacylglycerol substrates. In one aspect, <i>Mortierella ramanniana</i> DAGAT proteins have been isolated and have molecular weights of between approximately 36 and 37kDa as measured by SDS-PAGE. The invention also provides novel DAGAT polynucleotide and polypeptide sequences and to methods of producing such polypeptides using recombinant techniques. In addition, methods are provided for using such sequences to alter triacylglycerol levels in plants and to treat diseases associated with altered DAGAT activity or expression.

Claims (48)

1. An isolated DNA sequence encoding an enzyme active in the formation of triacylglycerol from diacylglycerol and fatty acyl substrates.
2. The isolated DNA sequence according to Claim 1, wherein said nucleic acid sequence encodes diacylglycerol acyltransferase.
3. The isolated DNA sequence according to Claim 1, wherein said nucleic acid sequence is isolated from a eukaryotic cell source.
4. The isolated DNA sequence according to Claim 3, wherein said eukaryotic cell source is selected from the group consisting of mammalian, nematode, fungal, and plant cells.
5. The DNA encoding sequence of Claim 4 wherein said diacylglycerol acyltransferase protein is from Mortierella ramanniana.
6. The DNA encoding sequence of Claim 4 wherein said cell source is selected from the group consisting of soybean, Brassica napus, Arabidopsis thaliana, corn, human, murine, Mortierella alpina, Aspergillus fumigatus, Aspergillus oraceus, Fuserium graminearum, Schzichytrium aggregatum, Caenorhabditis elegans, and Sacchromyces cervaisae.
7. The DNA encoding sequence of Claim 4 wherein said enzyme is selective for synthesis of a structured triacylglycerol.
8. The DNA encoding sequence of claim 7 wherein said structured triacylglycerol has a structure of the formula: S-U-S, wherein S represents a saturated fatty acid and U
represents an unsaturated fatty acid.
9. The DNA encoding sequence of Claim 4 wherein said diacylglycerol acyltransferase protein is encoded by a sequence which includes a nucleotide sequence selected from the group consisting of SEQ ID Nos: 46 through 72, inclusive.
10. An isolated polypeptide comprising the amino acid sequence of SEQ ID NO:
38.
11. An isolated polypeptide comprising the amino acid sequence of SEQ ID NO:
45.
12. An isolated polypeptide encoded by the nucleotide sequence as set forth in SEQ ID NO:37.
13. An isolated polypeptide encoded by the nucleotide sequence as set forth in SEQ ID NO:44.
14. An isolated polypeptide encoded by the nucleotide sequence as set forth in SEQ ID NO:84.
15. An isolated polynucleotide selected from the group consisting of:
a ) an isolated polynucleotide comprising a nucleotide sequence encoding the polypeptide of SEQ ID NO: 38;
b ) an isolated polynucleotide comprising SEQ ID NO: 37;
c ) an isolated polynucleotide comprising a nucleotide sequence which has at least 70%
identity to that of SEQ ID NO: 37 over the entire length of SEQ ID NO: 37;
d ) an isolated polynucleotide comprising a nucleotide sequence which has at least 80%
identity to that of SEQ ID NO: 37 over the entire length of SEQ ID NO: 37;
a ) an isolated polynucleotide comprising a nucleotide sequence which has at least 90%
identity to that of SEQ ID ID: 37 over the entire length of SEQ ID NO: 37;
f ) an isolated polynucleotide comprising a nucleotide sequence which has at least 95%
identity to that of SEQ ID NO: 37 over the entire length of SEQ ID NO: 37;
g ) an isolated polynucleotide that hybridizes, under stringent conditions, to SEQ ID NO:
37 or a fragment thereof; and h) an isolated polynucleotide complementary to the polynucleotide sequence of (a), (b), (c), (d), (e), (f), or (8).
16. An isolated polynucleotide selected from the group consisting of:
a) an isolated polynucleotide comprising SEQ ID NO: 84;
b) an isolated polynucleotide comprising a nucleotide sequence which has at least 70%
identity to that of SEQ ID NO: 84 over the entire length of SEQ ID NO: 84;
c) an isolated polynucleotide comprising a nucleotide sequence which has at least 80%
identity to that of SEQ ID NO: 84 over the entire length of SEQ ID NO: 84;
d) an isolated polynucleotide comprising a nucleotide sequence which has at least 90%
identity to that of SEQ ID NO: 84 over the entire length of SEQ ID NO: 84;
a) an isolated polynucleotide comprising a nucleotide sequence which has at least 95%
identity to that of SEQ ID NO: 84 over the entire length of SEQ ID NO: 84;
f) an isolated polynucleotide that hybridizes, under stringent conditions, to SEQ ID NO:
84 or a fragment thereof; and g) an isolated polynucleotide complementary to the polynucleotide sequence of (a), (b), (c), (d), (e), or (f).
17. An isolated, polynucleotide selected from the group consisting of:
a) an isolated polynucleotide comprising a nucleotide sequence encoding the polypeptide of SEQ ID NO: 45;
b) an isolated polynucleotide comprising SEQ ID NO: 44;
c) an isolated polynucleotide comprising a nucleotide sequence which has at least 70%
identity to that of SEQ ID NO: 44 over the entire length of SEQ ID NO: 44;
d) an isolated polynucleotide comprising a nucleotide sequence which has at least 80%
identity to that of SEQ ID NO: 44 over the entire length of SEQ ID NO: 44;
e) an isolated polynucleotide comprising a nucleotide sequence which has at least 90%
identity to that of SEQ ID NO: 44 over the entire length of SEQ ID NO: 44;
f) an isolated polynucleotide comprising a nucleotide sequence which has at least 95%
identity to that of SEQ ID NO: 44 over the entire length of SEQ ID NO: 44;
g) an isolated polynucleotide that hybridizes, under stringent conditions, to SEQ ID NO:
44 or a fragment thereof; and h) an isolated polynucleotide complementary to the polynucleotide sequence of (a), (b), (c), (d), (e), (f) or (g).
18. An isolated polynucleotide selected from the group consisting of SEQ ID
Nos:
46 through 67, inclusive.
19. A Mortierella acyltransferase protein wherein. said acyltransferase is active in the formation of triacylglycerol from diacylglycerol and fatty acyl substrates and wherein said acyltransferase is isolated from other proteins indigenous to Mortierella.
20. An acyltransferase protein, wherein said protean has. an apparent molecular mass of about 36kD on SDS-PAGE, said protein being substantially purified away from membranes and other proteins of the native cell and capable of catalyzing the production of triglycerides from diacylglycerol and fatty acyl substrates.
21. An acyltransferase protein, wherein said protein has an apparent molecular mass of about 36.5kD on SDS-PAGE, said protein being substantially purified away from membranes and other proteins of the native cell and capable of catalyzing the production of triglycerides from diacylglycerol and fatty acyl substrates.
22. A nucleic acid construct comprising as operably linked components in the 5' to 3' direction of transcription:
a transcriptional initiation region; and a polynucleotide sequence encoding an enzyme active in the formation of triacylglycerol from diacylglycerol and fatty acyl substrates
23. The nucleic acid construct according to Claim 22, wherein said enzyme is diacylglycerol acyltransferase.
24. A host cell comprising a DNA construct according to Claim 22.
25. The host cell according to Claim 24, wherein said host cell is selected from the group consisting of bacterial, insect, fungal, mammalian, and plant.
26. A plant comprising a cell of Claim 25.
27. A method for producing a recombinant host cell, comprising, transforming or transfecting a cell with a nucleic acid construct comprising a transcriptional initiation region and a polynucleotide sequence encoding an enzyme active in the formation of triacylglycerol from diacylglycerol and fatty acyl substrates, such that said host cell, under appropriate culture conditions, produces an acyltransferase protein.
28. The method according to claim 27 23 wherein said host cell is a plant cell.
29. A method for producing a recombinant host cell, comprising:
transforming or transfecting a cell with a nucleic acid construct comprising a transcriptional initiation region and a polynucleotide sequence selected from the group consisting of a polynucleotide according to claim i2 and a polynucleotide according to claim 13, such that said host cell, under appropriate culture conditions, produces an acyltransferase protein.
30. The method according to claim 29, wherein said polynucleotide sequence comprises the nucleotide sequence set forth in SEQ ID NO: 37.
31. The method according to claim 29, wherein said host cell is a plant cell.
32. A method of modifying the triacylglycerol composition in a plant cell, said method comprising:
transforming a plant cell with a nucleic acid construct comprising a transcriptional initiation region and a polynucleotide sequence encoding an enzyme active in the formation of triacylglycerol from diacylglycerol and fatty acyl substrates, or fragment or variant thereof
33. The method according to Claim 32, wherein said polynucleotide sequence is in an antisense orientation.
34. The method according to Claim 32, wherein sand polynucleotide sequence is in a sense orientation.
35. The method according to Claim 32, wherein tree activity of the endogenous diacylglycerol acyltransferase protein is suppressed.
36. The method according to Claim 32, wherein the activity of the endogenous diacylglycerol acyltransferase protein is enhanced.
37. A method of modifying the lipid composition in a plant cell, said method comprising:
transforming a plant cell with a nucleic acid construct comprising a transcriptional initiation region and a polynucleotide sequence selected from the group consisting of a polynucleotide according to claim l2 and a polynucleotide according to chum 13, such that said plant cell, under appropriate culture conditions, produces an acyltransferase protein
38. The method according to Claim 37 wherein said polynucleotide sequence is in an antisense orientation, whereby transcribed mRNA from sand sequence is complementary to the equivalent mRNA transcribed from the endogenous gene and the activity of said diacylglycerol acyltransferase protein in said plant cell is suppressed.
39. The method according to Claim 37, wherein said polynucleotide sequence is in a sense orientation.
40. An antibody immunospecific for the polypeptide of claim 10.
41. An antibody immunospecific for the polypeptide of claim 11.
42. A method of treating or ameliorating a disease or condition associated with altered diacylglycerol acyl transferase (DAGAT) activity, said method comprising the step of:
administering a therapeutically effective amount of an antagonist of DAGAT
activity to a subject in need thereof.
43. A method of treating or ameliorating a disease or condition associated with altered diacylglycerol acyl transferase (DAGAT) activity, said method comprising the step of:
administering a therapeutically effective amount of an agonist of DAGAT
activity to a subject in need thereof.
44. A method of diagnosing a disease or susceptibility to a disease associated with DAGAT expression or activity in a patient, said method comprising the step of:
determining the presence or absence of a mutation in the nucleotide sequence encoding DAGAT in the genome of said patient.
45. A method of diagnosing a disease or susceptibility to a disease associated with DAGAT expression or activity in a patient, said method comprising the step of:
detecting the presence or amount of DAGAT or an indicator thereof in a sample obtained from said patient.
46. A method for identifying a compound which stimulate or inhibit DAGAT
expression or activity comprising:
contacting a composition comprising a polypeptide selected from the group consisting of a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 38 and a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 45 with a candidate compound; and detecting an interaction between said polypeptide and said candidate compound.
47. An agonist of a polypeptide selected from the group consisting of a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 38 and a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 45.
48. An antagonist of a polypeptide selected from the group consisting of a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 38 and a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 45.
CA2331329A 1998-07-02 1999-06-30 Diacylglycerol acyl transferase proteins Expired - Fee Related CA2331329C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US9163198P 1998-07-02 1998-07-02
US60/091,631 1998-07-02
US13082999P 1999-04-23 1999-04-23
US60/130,829 1999-04-23
PCT/US1999/015243 WO2000001713A2 (en) 1998-07-02 1999-06-30 Diacylglycerol acyl transferase proteins

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CA2331329A1 true CA2331329A1 (en) 2000-01-13
CA2331329C CA2331329C (en) 2011-08-30

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US (1) US6822141B2 (en)
EP (1) EP1098962B1 (en)
JP (1) JP4514952B2 (en)
AT (1) ATE442435T1 (en)
BR (1) BR9911800A (en)
CA (1) CA2331329C (en)
DE (1) DE69941390D1 (en)
WO (1) WO2000001713A2 (en)

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