CA2343279A1 - Scanning confocal optical microscope system - Google Patents

Scanning confocal optical microscope system Download PDF

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Publication number
CA2343279A1
CA2343279A1 CA002343279A CA2343279A CA2343279A1 CA 2343279 A1 CA2343279 A1 CA 2343279A1 CA 002343279 A CA002343279 A CA 002343279A CA 2343279 A CA2343279 A CA 2343279A CA 2343279 A1 CA2343279 A1 CA 2343279A1
Authority
CA
Canada
Prior art keywords
microscope system
optical microscope
scanning confocal
confocal optical
spread
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002343279A
Other languages
French (fr)
Other versions
CA2343279C (en
Inventor
William Bradshaw Amos
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Medical Research Council
Original Assignee
Medical Research Council
William Bradshaw Amos
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Research Council, William Bradshaw Amos filed Critical Medical Research Council
Publication of CA2343279A1 publication Critical patent/CA2343279A1/en
Application granted granted Critical
Publication of CA2343279C publication Critical patent/CA2343279C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/30—Measuring the intensity of spectral lines directly on the spectrum itself
    • G01J3/36—Investigating two or more bands of a spectrum by separate detectors
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0064—Optical details of the image generation multi-spectral or wavelength-selective arrangements, e.g. wavelength fan-out, chromatic profiling

Abstract

A confocal scanning optical microscope system wherein a dispersive optical device such as a prism (28) separates an image from a restricted area of a specimen into a spread of images of differing wavelengths. Different parts of the spread of images are focussed at respective beam-limiting apertures (34, 36) which are conjugate with one another, and the spectrally differing beams passing through the beam-limiting apertures pass to respective detectors (35, 37).
CA002343279A 1998-11-19 1999-07-15 Scanning confocal optical microscope system Expired - Fee Related CA2343279C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB9825267.9 1998-11-19
GBGB9825267.9A GB9825267D0 (en) 1998-11-19 1998-11-19 Scanning confocal optical microscope system
PCT/GB1999/002284 WO2000031577A1 (en) 1998-11-19 1999-07-15 Scanning confocal optical microscope system

Publications (2)

Publication Number Publication Date
CA2343279A1 true CA2343279A1 (en) 2000-06-02
CA2343279C CA2343279C (en) 2006-01-31

Family

ID=10842647

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002343279A Expired - Fee Related CA2343279C (en) 1998-11-19 1999-07-15 Scanning confocal optical microscope system

Country Status (11)

Country Link
US (1) US6555811B1 (en)
EP (1) EP1131662B1 (en)
JP (1) JP2002530716A (en)
AT (1) ATE220215T1 (en)
AU (1) AU765975B2 (en)
CA (1) CA2343279C (en)
DE (1) DE69902053T2 (en)
DK (1) DK1131662T3 (en)
ES (1) ES2179665T3 (en)
GB (2) GB9825267D0 (en)
WO (1) WO2000031577A1 (en)

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US6958811B2 (en) 2000-06-29 2005-10-25 Carl Zeiss Jena Gmbh Method for the detection of dyes in fluorescence microscopy
US6747737B2 (en) 2000-06-29 2004-06-08 Carl Zeiss Jena Gmbh Method for optical detection of an illuminated specimen in a plurality of detection channels
DE20012378U1 (en) 2000-07-17 2000-10-19 Leica Microsystems Arrangement for spectrally sensitive incident and transmitted light illumination
DE10038049A1 (en) * 2000-08-02 2002-02-14 Leica Microsystems Optical arrangement for the selection and detection of the spectral range of a light beam
US6947133B2 (en) 2000-08-08 2005-09-20 Carl Zeiss Jena Gmbh Method for increasing the spectral and spatial resolution of detectors
US6858852B2 (en) 2000-08-08 2005-02-22 Carl Zeiss Jena Gmbh Method and apparatus for rapid change of fluorescence bands in the detection of dyes in fluorescence microscopy
WO2002014811A1 (en) * 2000-08-17 2002-02-21 Mg Microscope Gmbh Light detector unit and method for detecting luminescence light and confocal microscope for luminescence microscopy
JP2002277744A (en) * 2001-03-21 2002-09-25 Olympus Optical Co Ltd Scanning type optical microscope
JP4939703B2 (en) * 2001-08-21 2012-05-30 オリンパス株式会社 Scanning laser microscope
US6750457B2 (en) * 2001-08-29 2004-06-15 Becton Dickinson And Company System for high throughput analysis
DE10156695B4 (en) * 2001-11-17 2004-07-15 Leica Microsystems Heidelberg Gmbh Scanning microscope, scanning microscopy method and bandpass filter
DE10213187A1 (en) * 2002-03-23 2003-10-09 Leica Microsystems Spectral analysis method and scanning microscope
DE10238100A1 (en) * 2002-08-21 2004-03-04 Leica Microsystems Heidelberg Gmbh Device for spectral selection and detection of a light beam and scanning microscope
DE60303613T2 (en) * 2002-08-30 2006-08-17 Medical Research Council OPTICAL PROJECTION TOMOGRAPHY
JP4086182B2 (en) * 2002-10-09 2008-05-14 オリンパス株式会社 Spectroscope, confocal optical system using the same, and scanning optical microscope
DE10257120B4 (en) * 2002-12-05 2020-01-16 Leica Microsystems Cms Gmbh Scanning microscope for imaging an object
JP4677728B2 (en) 2004-03-22 2011-04-27 株式会社ニコン Confocal microscope and confocal microscope system
JP4804727B2 (en) * 2004-06-24 2011-11-02 オリンパス株式会社 Optical scanning confocal microscope
DE102004047820A1 (en) * 2004-09-29 2006-03-30 Leica Microsystems Cms Gmbh Scanning microscope and scanning microscopy method
DE102005000915A1 (en) * 2005-01-06 2006-07-20 Leica Microsystems Cms Gmbh Device for the multifocal confocal miroscopic determination of the spatial distribution and the multifocal fluctuation analysis of fluorescent molecules and structures with spectrally flexible detection
EP1906224B1 (en) * 2005-07-21 2012-01-25 Nikon Corporation Confocal Microscope Apparatus
US7397561B2 (en) * 2005-11-07 2008-07-08 Wafermasters, Incorporated Spectroscopy system
US7564547B2 (en) * 2005-11-07 2009-07-21 Wafermasters, Inc. Spectroscopy system
US20070132831A1 (en) * 2005-12-13 2007-06-14 Bio-Rad Laboratories, Inc. Masking to prevent overexposure and light spillage in microarray scanning
KR100790702B1 (en) * 2006-09-08 2008-01-02 삼성전기주식회사 Confocal electroluminescence spectral-microscope
GB2451442B (en) * 2007-07-30 2013-03-06 Lein Applied Diagnostics Ltd Optical measurement apparatus and method therefor
JP5541972B2 (en) 2010-06-09 2014-07-09 オリンパス株式会社 Scanning confocal microscope
US8456637B2 (en) * 2010-08-26 2013-06-04 Mitutoyo Corporation Multiple measuring point configuration for a chromatic point sensor
DE102014116782A1 (en) 2014-11-17 2016-05-19 Carl Zeiss Microscopy Gmbh Detector device for a microscope
JP2022526650A (en) * 2019-04-12 2022-05-25 インベニオ イメージング、インコーポレイテッド Imaging system for detection of intraoperative contrast media in tissue

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2626383B1 (en) 1988-01-27 1991-10-25 Commissariat Energie Atomique EXTENDED FIELD SCAN AND DEPTH CONFOCAL OPTICAL MICROSCOPY AND DEVICES FOR CARRYING OUT THE METHOD
US5032720A (en) * 1988-04-21 1991-07-16 White John G Confocal imaging system
GB9014263D0 (en) * 1990-06-27 1990-08-15 Dixon Arthur E Apparatus and method for spatially- and spectrally- resolvedmeasurements
US5886784A (en) * 1993-09-08 1999-03-23 Leica Lasertechink Gmbh Device for the selection and detection of at least two spectral regions in a beam of light
DE4330347C2 (en) * 1993-09-08 1998-04-09 Leica Lasertechnik Use of a device for the selection and detection of at least two spectral ranges of a light beam
JP3568626B2 (en) * 1994-05-24 2004-09-22 オリンパス株式会社 Scanning optical microscope
DE4419940A1 (en) * 1994-06-08 1995-12-14 Eberhard Dipl Phys Tuengler Three=dimensional image recording for confocal light microscopy
DE19510102C1 (en) * 1995-03-20 1996-10-02 Rainer Dr Uhl Confocal fluorescence microscope
JP2000097858A (en) * 1998-09-22 2000-04-07 Olympus Optical Co Ltd Scanning type confocal microscope
JP2000199855A (en) * 1998-11-02 2000-07-18 Olympus Optical Co Ltd Scanning type optical microscopic device

Also Published As

Publication number Publication date
AU765975B2 (en) 2003-10-09
ES2179665T3 (en) 2003-01-16
DE69902053D1 (en) 2002-08-08
GB2344014A (en) 2000-05-24
DE69902053T2 (en) 2002-11-07
ATE220215T1 (en) 2002-07-15
GB2344014B (en) 2001-02-07
GB9825267D0 (en) 1999-01-13
EP1131662A1 (en) 2001-09-12
US6555811B1 (en) 2003-04-29
JP2002530716A (en) 2002-09-17
EP1131662B1 (en) 2002-07-03
CA2343279C (en) 2006-01-31
AU4923099A (en) 2000-06-13
WO2000031577A1 (en) 2000-06-02
GB9916494D0 (en) 1999-09-15
DK1131662T3 (en) 2002-10-14

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