CA2343377A1 - Target analyte sensors utilizing microspheres - Google Patents

Target analyte sensors utilizing microspheres Download PDF

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Publication number
CA2343377A1
CA2343377A1 CA002343377A CA2343377A CA2343377A1 CA 2343377 A1 CA2343377 A1 CA 2343377A1 CA 002343377 A CA002343377 A CA 002343377A CA 2343377 A CA2343377 A CA 2343377A CA 2343377 A1 CA2343377 A1 CA 2343377A1
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Prior art keywords
microspheres
substrate
sites
composition according
bioactive agent
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Granted
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CA002343377A
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French (fr)
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CA2343377C (en
Inventor
David R. Walt
Karri L. Michael
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Tufts University
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Expired - Lifetime legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/7703—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator using reagent-clad optical fibres or optical waveguides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00646—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports
    • B01J2219/00648—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports by the use of solid beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00725—Peptides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • G01N15/1433—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1468—Electro-optical investigation, e.g. flow cytometers with spatial resolution of the texture or inner structure of the particle
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1434—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
    • G01N2015/1438—Using two lasers in succession
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N2035/0097—Control arrangements for automatic analysers monitoring reactions as a function of time
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S359/00—Optical: systems and elements
    • Y10S359/90—Methods
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/808—Optical sensing apparatus

Abstract

A microsphere-based analytic chemistry system and method for making the same is disclosed in which microspheres or particles carrying bioactive agents ma y be combined randomly or in ordered fashion and dispersed on a substrate to form an array while maintaining the ability to identify the location of bioactive agents and particles within the array using an optically interrogatable, optical signature encoding scheme. A wide variety of modifie d substrates may be employed which provide either discrete or non-discrete sit es for accomodating the microspheres in either random or patterned distribution s. The substrates may be constructed from a variety of materials to form either two-dimensional or three-dimensional configurations. In a preferred embodiment, a modified fiber optic bundle or array is employed as a substrat e to produce a high density array. The disclosed system and method have utilit y for detecting target analytes and screening large libraries of bioactive age nt.

Claims (17)

1. A composition comprising:
a) a substrate with a surface comprising discrete sites, wherein said substrate is not a fiber optic bundle; and b) a population of microspheres comprising at least as first and a second subpopulation each comprising:
i) a first and a second bioactive agent, respectively; and ii) a first and a second optical signature, respectively, capable of identifying said bioactive agent;
wherein said microspheres are distributed on said sites.
2. A composition according to claim 1 wherein said sites comprise wells.
3. A composition according to claim 1 or 2 wherein said substrate is glass.
4. A composition according to claim 1, 2 or 3 wherein said substrate is plastic.
5. A composition according to claim 1, 2, 3 or 4 wherein said sites comprise chemically functionalized sites.
6. A composition according to claim 1, 2, 3, 4 or 5 wherein said bioactive agents comprise nucleic acids.
7. A composition according to claim 1, 2, 3, 4 or 5 wherein said bioactive agents comprise proteins.
8. A composition according to claim 7 wherein said proteins are selected from the group consisting of enzymes and antibodies.
9. A composition according to claim 1, 2, 3, 4, 5, 6, 7 or 8 wherein said at least one of said optical signatures comprises at least one fluorescent dye.
10. A composition according to claim 1, 2, 3, 4, 5, 6, 7, 8 or 9 wherein at least one of said optical signatures comprises at least two fluorescent dyes.
11. A method of determining the presence of a target analyte in a sample comprising:
a) contacting said sample with a composition comprising:
i) a substrate with a surface comprising discrete sites, wherein said substrate is not an fiber optic bundle; and ii) a population of microspheres comprising at least a first and a second subpopulation each comprising:
1) a bioactive agent; and 2) an optical signature capable of identifying said bioactive agent;
wherein said microspheres are distributed on said surface; and b) determining the presence or absence of said target analyte.
12. A method according to claim 11 further comprising identifying the location of each bioactive agent on said substrate.
13. A method of making a composition comprising:
a) forming a surface comprising individual sites on a substrate, wherein said substrate is not a fiber optic bundle; and b) distributing microspheres on said surface such that said individual sites contain microspheres, wherein said microspheres comprise at least a first and a second subpopulations each comprising:
i) a bioactive agent; and ii) an optical signature capable of identifying said bioactive agent.
14. A method according to claim 13 wherein said distributing comprises serially adding said subpopulations to said sites.
15. A method according to claim 11, 12 or 13 wherein said bioactive agents are nucleic acids.
16. A method according to claim 12 or 13 wherein said bioactive agents are proteins.
17. A method according to claim 11, 12, 13, 14, 15 or 16 wherein said sites are wells.
CA2343377A 1998-09-11 1999-09-10 Target analyte sensors utilizing microspheres Expired - Lifetime CA2343377C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/151,877 1998-09-11
US09/151,877 US6327410B1 (en) 1997-03-14 1998-09-11 Target analyte sensors utilizing Microspheres
PCT/US1999/020914 WO2000016101A2 (en) 1998-09-11 1999-09-10 Target analyte sensors utilizing microspheres

Publications (2)

Publication Number Publication Date
CA2343377A1 true CA2343377A1 (en) 2000-03-23
CA2343377C CA2343377C (en) 2011-11-22

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CA2343377A Expired - Lifetime CA2343377C (en) 1998-09-11 1999-09-10 Target analyte sensors utilizing microspheres

Country Status (6)

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US (2) US6327410B1 (en)
EP (1) EP1125127A2 (en)
JP (1) JP3836323B2 (en)
AU (2) AU773211B2 (en)
CA (1) CA2343377C (en)
WO (1) WO2000016101A2 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9759658B2 (en) 2014-08-08 2017-09-12 Quantum-Si Incorporated Integrated device for temporal binning of received photons
US9863880B2 (en) 2013-11-17 2018-01-09 Quantum-Si Incorporated Optical system and assay chip for probing, detecting and analyzing molecules
US9885657B2 (en) 2014-08-08 2018-02-06 Quantum-Si Incorporated Integrated device with external light source for probing detecting and analyzing molecules
US9921157B2 (en) 2014-08-08 2018-03-20 Quantum-Si Incorporated Optical system and assay chip for probing, detecting and analyzing molecules

Families Citing this family (460)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE43097E1 (en) 1994-10-13 2012-01-10 Illumina, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US6958245B2 (en) 1996-04-25 2005-10-25 Bioarray Solutions Ltd. Array cytometry
US6387707B1 (en) * 1996-04-25 2002-05-14 Bioarray Solutions Array Cytometry
ES2288760T3 (en) * 1996-04-25 2008-01-16 Bioarray Solutions Ltd. ELECTROCINETIC ASSEMBLY CONTROLLED BY LIGHT OF PARTICLES NEXT TO SURFACES.
US7041510B2 (en) 1996-04-25 2006-05-09 Bioarray Solutions Ltd. System and method for programmable illumination pattern generation
US7144119B2 (en) * 1996-04-25 2006-12-05 Bioarray Solutions Ltd. System and method for programmable illumination pattern generation
US20030027126A1 (en) 1997-03-14 2003-02-06 Walt David R. Methods for detecting target analytes and enzymatic reactions
US7622294B2 (en) * 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US6023540A (en) 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
US6327410B1 (en) * 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
JP4294740B2 (en) 1997-05-23 2009-07-15 ソレクサ・インコーポレイテッド System and apparatus for serial processing of analytes
US7348181B2 (en) 1997-10-06 2008-03-25 Trustees Of Tufts College Self-encoding sensor with microspheres
US7115884B1 (en) * 1997-10-06 2006-10-03 Trustees Of Tufts College Self-encoding fiber optic sensor
US6749811B2 (en) 1998-04-28 2004-06-15 The Johns Hopkins University Molecularly imprinted polymer solution anion sensor
JP3662850B2 (en) * 1998-06-24 2005-06-22 イルミナ インコーポレイテッド Decoding array sensors with microspheres
US6429027B1 (en) 1998-12-28 2002-08-06 Illumina, Inc. Composite arrays utilizing microspheres
US7612020B2 (en) * 1998-12-28 2009-11-03 Illumina, Inc. Composite arrays utilizing microspheres with a hybridization chamber
DE60031506T2 (en) 1999-01-08 2007-08-23 Applera Corp., Foster City FASERMATRIX FOR MEASURING CHEMICALS, AND METHOD FOR THE PRODUCTION AND USE THEREOF
US7595189B2 (en) 1999-01-08 2009-09-29 Applied Biosystems, Llc Integrated optics fiber array
JP2000262460A (en) * 1999-03-18 2000-09-26 Inst Of Physical & Chemical Res Method for detecting specified location, method for biological measurement, device for detecting specified location of biological sample, and optical fiber holding device
US20050100943A1 (en) * 2000-04-11 2005-05-12 Hideki Kambara Method of producing probe arrays for biological materials using fine particles
US20050181440A1 (en) * 1999-04-20 2005-08-18 Illumina, Inc. Nucleic acid sequencing using microsphere arrays
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
EP1196630B2 (en) 1999-04-20 2018-10-17 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US6544732B1 (en) 1999-05-20 2003-04-08 Illumina, Inc. Encoding and decoding of array sensors utilizing nanocrystals
AU7569600A (en) 1999-05-20 2000-12-28 Illumina, Inc. Combinatorial decoding of random nucleic acid arrays
US8481268B2 (en) 1999-05-21 2013-07-09 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US8080380B2 (en) 1999-05-21 2011-12-20 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
DK1218545T3 (en) 1999-08-18 2012-02-20 Illumina Inc Methods of Preparation of Oligonucleotide Solutions
WO2001018524A2 (en) 1999-08-30 2001-03-15 Illumina, Inc. Methods for improving signal detection from an array
US7211390B2 (en) * 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7244559B2 (en) * 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
EP1122535A3 (en) * 2000-01-31 2004-09-22 The Penn State Research Foundation Interrogation of changes in the contents of a sealed container
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US6913884B2 (en) 2001-08-16 2005-07-05 Illumina, Inc. Compositions and methods for repetitive use of genomic DNA
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
WO2001057269A2 (en) * 2000-02-07 2001-08-09 Illumina, Inc. Nucleic acid detection methods using universal priming
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
CA2399908A1 (en) * 2000-02-10 2001-08-16 Todd Dickinson Array of individual arrays as substrate for bead-based simultaneous processing of samples and manufacturing method therefor
US6770441B2 (en) * 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
EP1967595A3 (en) 2000-02-16 2008-12-03 Illumina, Inc. Parallel genotyping of multiple patient samples
US20010026920A1 (en) * 2000-02-25 2001-10-04 Mark Chandler Internal standards and controls for multiplexed assay
WO2001071318A1 (en) * 2000-03-21 2001-09-27 The Board Of Trustees Of The University Of Illinois Colorimetric artificial nose having an array of dyes and method for artificial olfaction
US6844154B2 (en) * 2000-04-04 2005-01-18 Polygenyx, Inc. High throughput methods for haplotyping
DE60117556T2 (en) 2000-06-21 2006-11-02 Bioarray Solutions Ltd. MULTI-ANALYTIC MOLECULAR ANALYSIS THROUGH THE USE OF APPLICATION SPECIFIC RAPID PARTICLE ARRAYS
US9709559B2 (en) 2000-06-21 2017-07-18 Bioarray Solutions, Ltd. Multianalyte molecular analysis using application-specific random particle arrays
AU7166201A (en) * 2000-06-28 2002-01-08 Illumina Inc Composite arrays utilizing microspheres with a hybridization chamber
AU2001284760A1 (en) * 2000-08-09 2002-02-18 Illumina, Inc. Automated information processing in randomly ordered arrays
US6934408B2 (en) * 2000-08-25 2005-08-23 Amnis Corporation Method and apparatus for reading reporter labeled beads
US7057704B2 (en) * 2000-09-17 2006-06-06 Bioarray Solutions Ltd. System and method for programmable illumination pattern generation
US20030045005A1 (en) * 2000-10-17 2003-03-06 Michael Seul Light-controlled electrokinetic assembly of particles near surfaces
CN1231758C (en) * 2000-10-19 2005-12-14 泰博特克公司 Method and device for manipulation of microcarriers for identification purpose
US20040018491A1 (en) * 2000-10-26 2004-01-29 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
EP1366364A4 (en) 2001-03-07 2006-05-17 Bio Rad Laboratories Assay system for simultaneous detection and measurement of multiple modified cellular proteins
DE60127821D1 (en) * 2001-05-25 2007-05-24 Corning Inc Method for the determination of the reactions and the metabolic activity with fluorescence temperature-sensitive material
US7262063B2 (en) * 2001-06-21 2007-08-28 Bio Array Solutions, Ltd. Directed assembly of functional heterostructures
CN100399025C (en) * 2001-09-27 2008-07-02 北京源德生物医学工程有限公司 Immununity and gene alaysis system by multiple mark continuous injections
JP4754746B2 (en) * 2001-09-28 2011-08-24 オリンパス株式会社 Rod-shaped carrier and cylinder reaction vessel equipped with the same
ES2661167T3 (en) 2001-10-15 2018-03-27 Bioarray Solutions Ltd. Multiplexed analysis of polymorphic loci by simultaneous consultation and enzyme-mediated detection
US6551788B1 (en) * 2001-11-28 2003-04-22 Beckman Coulter, Inc. Particle-based ligand assay with extended dynamic range
US7011945B2 (en) * 2001-12-21 2006-03-14 Eastman Kodak Company Random array of micro-spheres for the analysis of nucleic acids
US7335153B2 (en) 2001-12-28 2008-02-26 Bio Array Solutions Ltd. Arrays of microparticles and methods of preparation thereof
AU2003215240A1 (en) * 2002-02-14 2003-09-04 Illumina, Inc. Automated information processing in randomly ordered arrays
US20030162178A1 (en) * 2002-02-25 2003-08-28 O'hagan David Variable microarray and methods of detecting one or more anlaytes in a sample
US20030162179A1 (en) * 2002-02-27 2003-08-28 General Electric Company Fabrication, performance testing, and screening of three dimensional arrays of materials
US7491491B2 (en) * 2002-03-12 2009-02-17 Polytechnic Institute Of New York University Detecting and/or measuring a substance based on a resonance shift of photons orbiting within a microsphere
US6916620B2 (en) * 2002-03-15 2005-07-12 Eastman Kodak Company Random array of micro-spheres for the analysis of nucleic acid using enzyme digestion
US20030232384A1 (en) * 2002-06-13 2003-12-18 Eastman Kodak Company Microarray system utilizing microtiter plates
CN100414296C (en) * 2002-06-14 2008-08-27 赵翀 Embedded high-pass three-dimensional biological detecting technique and agent box
US7217518B2 (en) * 2002-08-02 2007-05-15 Applera Corporation Fluorescence polarization assay
CA2496840A1 (en) * 2002-08-28 2004-04-29 Bionexus Ventures L.L.C. Screening antibodies using an optical fiber array device capable of simultaneously performing multiple functional assays
US7595883B1 (en) * 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
US7122384B2 (en) * 2002-11-06 2006-10-17 E. I. Du Pont De Nemours And Company Resonant light scattering microparticle methods
AU2003291718A1 (en) * 2002-11-15 2004-06-15 University Of Maryland, Baltimore Release of the self-quenching of fluorescence near silver metallic surfaces
US7526114B2 (en) 2002-11-15 2009-04-28 Bioarray Solutions Ltd. Analysis, secure access to, and transmission of array images
WO2004065000A1 (en) 2003-01-21 2004-08-05 Illumina Inc. Chemical reaction monitor
US20040175843A1 (en) * 2003-03-04 2004-09-09 Roitman Daniel B. Near-field and far-field encoding and shaping of microbeads for bioassays
US20040175842A1 (en) * 2003-03-04 2004-09-09 Roitman Daniel B. Near-field and far-field encoding of microbeads for bioassays
WO2004097371A2 (en) * 2003-04-25 2004-11-11 Board Of Regents, The University Of Texas System System and method for the detection of analytes
US8150627B2 (en) 2003-05-15 2012-04-03 Illumina, Inc. Methods and compositions for diagnosing lung cancer with specific DNA methylation patterns
US8150626B2 (en) * 2003-05-15 2012-04-03 Illumina, Inc. Methods and compositions for diagnosing lung cancer with specific DNA methylation patterns
WO2004104922A2 (en) * 2003-05-16 2004-12-02 Board Of Regents, The University Of Texas System Image and part recognition technology
US9317922B2 (en) 2003-05-16 2016-04-19 Board Of Regents The University Of Texas System Image and part recognition technology
US20050181394A1 (en) * 2003-06-20 2005-08-18 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
EP1636337A4 (en) 2003-06-20 2007-07-04 Illumina Inc Methods and compositions for whole genome amplification and genotyping
US20040259100A1 (en) * 2003-06-20 2004-12-23 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
WO2005029705A2 (en) 2003-09-18 2005-03-31 Bioarray Solutions, Ltd. Number coding for identification of subtypes of coded types of solid phase carriers
AU2004276761B2 (en) 2003-09-22 2009-12-24 Bioarray Solutions, Ltd. Surface immobilized polyelectrolyte with multiple functional groups capable of covalently bonding to biomolecules
JP2005098877A (en) * 2003-09-25 2005-04-14 Hitachi Software Eng Co Ltd Antibody detector, its manufacturing method, and antibody detecting method
AU2004286252A1 (en) 2003-10-28 2005-05-12 Bioarray Solutions Ltd. Optimization of gene expression analysis using immobilized capture probes
AU2004287069B2 (en) 2003-10-29 2009-07-16 Bioarray Solutions, Ltd. Multiplexed nucleic acid analysis by fragmentation of double-stranded DNA
US7390628B2 (en) * 2003-12-23 2008-06-24 University Of Florida Research Foundation, Inc. Microparticle-based diagnostic methods
US20050136414A1 (en) * 2003-12-23 2005-06-23 Kevin Gunderson Methods and compositions for making locus-specific arrays
US20060257854A1 (en) * 2004-02-27 2006-11-16 Mcdevitt John T Membrane assay system including preloaded particles
WO2005083423A2 (en) * 2004-02-27 2005-09-09 Board Of Regents, The University Of Texas System System and method for integrating fluids and reagents in self-contained cartridges containing particle and membrane sensor elements
US8101431B2 (en) 2004-02-27 2012-01-24 Board Of Regents, The University Of Texas System Integration of fluids and reagents into self-contained cartridges containing sensor elements and reagent delivery systems
US7781226B2 (en) * 2004-02-27 2010-08-24 The Board Of Regents Of The University Of Texas System Particle on membrane assay system
WO2005085850A1 (en) * 2004-02-27 2005-09-15 Tianxin Wang Methods for multiplexed analyte detection
US8105849B2 (en) 2004-02-27 2012-01-31 Board Of Regents, The University Of Texas System Integration of fluids and reagents into self-contained cartridges containing sensor elements
US20050208504A1 (en) * 2004-03-17 2005-09-22 Srinka Ghosh Method and system for testing feature-extractability of high-density microarrays using an embedded pattern block
US7776531B1 (en) 2004-03-25 2010-08-17 Illumina, Inc. Compositions and methods for stabilizing surface bound probes
WO2005093416A1 (en) * 2004-03-26 2005-10-06 Japan Science And Technology Agency Substrate for disposing beads and bead disposing method using the same
JP2005306171A (en) * 2004-04-20 2005-11-04 Yamaha Motor Co Ltd Saddle ride type vehicle
US7622281B2 (en) * 2004-05-20 2009-11-24 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for clonal amplification of nucleic acid
EP1602928A1 (en) * 2004-06-01 2005-12-07 Universiteit Maastricht Process and kit for determining binding parameters of bioaffinity binding reactions
FR2871358B1 (en) * 2004-06-14 2007-02-09 Mauna Kea Technologies Soc Par METHOD AND SYSTEM FOR MICROSCOPIC MULTI-MARKING FIBER FLUORESCENCE IMAGING
US7692219B1 (en) 2004-06-25 2010-04-06 University Of Hawaii Ultrasensitive biosensors
US7702466B1 (en) 2004-06-29 2010-04-20 Illumina, Inc. Systems and methods for selection of nucleic acid sequence probes
US7697808B2 (en) * 2004-07-27 2010-04-13 Ut-Battelle, Llc Multi-tipped optical component
US7697807B2 (en) * 2006-06-01 2010-04-13 Ut-Battelle, Llc Multi-tipped optical component
US7848889B2 (en) 2004-08-02 2010-12-07 Bioarray Solutions, Ltd. Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification
EP1787107A2 (en) * 2004-08-19 2007-05-23 Blood Cell Storage, Inc. FLUORESCENT pH DETECTOR SYSTEM AND RELATED METHODS
US8183052B2 (en) * 2004-08-19 2012-05-22 Blood Cell Storage, Inc. Methods and apparatus for sterility testing
US8497134B2 (en) 2004-08-19 2013-07-30 Blood Cell Storage, Inc. Fluorescent detector systems for the detection of chemical perturbations in sterile storage devices
WO2006047038A1 (en) * 2004-09-28 2006-05-04 Trustees Of Tufts College Apparatus and method for cell migration assays
US7785785B2 (en) 2004-11-12 2010-08-31 The Board Of Trustees Of The Leland Stanford Junior University Charge perturbation detection system for DNA and other molecules
EP1825267A4 (en) * 2004-11-29 2008-07-09 Perkinelmer Life & Analytical Sciences Prticle-based multiplex assay for identifying glycosylation
US7647186B2 (en) * 2004-12-07 2010-01-12 Illumina, Inc. Oligonucleotide ordering system
GB0505971D0 (en) * 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
US20060246576A1 (en) 2005-04-06 2006-11-02 Affymetrix, Inc. Fluidic system and method for processing biological microarrays in personal instrumentation
AU2006309284B2 (en) 2005-05-31 2012-08-02 Board Of Regents, The University Of Texas System Methods and compositions related to determination and use of white blood cell counts
US8486629B2 (en) 2005-06-01 2013-07-16 Bioarray Solutions, Ltd. Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation
EP2278303A3 (en) * 2005-06-10 2012-02-22 Life Technologies Corporation Method and system for multiplex genetic analysis
CA2611743C (en) * 2005-06-15 2019-12-31 Callida Genomics, Inc. Nucleic acid analysis by forming and tracking aliquoted fragments of a target polynucleotide
NO20053373D0 (en) 2005-07-11 2005-07-11 Rikshospitalet Radiumhospitale Multicolored Particles.
US7781224B2 (en) * 2005-08-10 2010-08-24 Lawrence Livermore National Security, Llc Safe biodegradable fluorescent particles
US7996188B2 (en) 2005-08-22 2011-08-09 Accuri Cytometers, Inc. User interface for a flow cytometer system
US7595473B2 (en) * 2005-08-22 2009-09-29 Tufts University Method and system of array imaging
US8017402B2 (en) 2006-03-08 2011-09-13 Accuri Cytometers, Inc. Fluidic system for a flow cytometer
US8303894B2 (en) 2005-10-13 2012-11-06 Accuri Cytometers, Inc. Detection and fluidic system of a flow cytometer
US8117902B2 (en) * 2005-11-03 2012-02-21 University Of Massachusetts Nanopatterned surfaces and related methods for selective adhesion, sensing and separation
US7329860B2 (en) 2005-11-23 2008-02-12 Illumina, Inc. Confocal imaging methods and apparatus
US20070127863A1 (en) * 2005-12-07 2007-06-07 Accuri Instruments Inc. System and method for guiding light from an interrogation zone to a detector system
US11237171B2 (en) 2006-02-21 2022-02-01 Trustees Of Tufts College Methods and arrays for target analyte detection and determination of target analyte concentration in solution
US8460879B2 (en) 2006-02-21 2013-06-11 The Trustees Of Tufts College Methods and arrays for target analyte detection and determination of target analyte concentration in solution
US8149402B2 (en) * 2006-02-22 2012-04-03 Accuri Cytometers, Inc. Optical system for a flow cytometer
US8031340B2 (en) * 2006-02-22 2011-10-04 Accuri Cytometers, Inc. Optical system for a flow cytometer
US8283177B2 (en) 2006-03-08 2012-10-09 Accuri Cytometers, Inc. Fluidic system with washing capabilities for a flow cytometer
US7780916B2 (en) 2006-03-08 2010-08-24 Accuri Cytometers, Inc. Flow cytometer system with unclogging feature
US20070224684A1 (en) * 2006-03-22 2007-09-27 Olson David C Transportable flow cytometer
US7914988B1 (en) 2006-03-31 2011-03-29 Illumina, Inc. Gene expression profiles to predict relapse of prostate cancer
US20070238140A1 (en) * 2006-04-07 2007-10-11 Pentoney Stephen L Jr Method For Multiplex Bead-Based Assays Using Chemiluminescence and Fluorescence
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
WO2008014485A2 (en) 2006-07-28 2008-01-31 California Institute Of Technology Multiplex q-pcr arrays
WO2008016335A1 (en) 2006-08-03 2008-02-07 National University Of Singapore A microarray system and a process for producing microarrays
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
GB0618514D0 (en) * 2006-09-20 2006-11-01 Univ Nottingham Trent Method of detecting interactions on a microarray using nuclear magnetic resonance
US8715573B2 (en) 2006-10-13 2014-05-06 Accuri Cytometers, Inc. Fluidic system for a flow cytometer with temporal processing
WO2008058217A2 (en) * 2006-11-07 2008-05-15 Accuri Instruments Inc. Flow cell for a flow cytometer system
US7813013B2 (en) * 2006-11-21 2010-10-12 Illumina, Inc. Hexagonal site line scanning method and system
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
CN101669026B (en) 2006-12-14 2014-05-07 生命技术公司 Methods and apparatus for measuring analytes using large scale FET arrays
US11339430B2 (en) 2007-07-10 2022-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
FR2910632B1 (en) * 2006-12-22 2010-08-27 Commissariat Energie Atomique OPTICAL PLASMON ENCODING DEVICE AND AUTHENTICATION METHOD EMPLOYING THE SAME
US7739060B2 (en) * 2006-12-22 2010-06-15 Accuri Cytometers, Inc. Detection system and user interface for a flow cytometer system
US20080243865A1 (en) * 2007-03-28 2008-10-02 Oracle International Corporation Maintaining global state of distributed transaction managed by an external transaction manager for clustered database systems
US20080274458A1 (en) * 2007-05-01 2008-11-06 Latham Gary J Nucleic acid quantitation methods
CN101720359A (en) * 2007-06-01 2010-06-02 454生命科学公司 System and meth0d for identification of individual samples from a multiplex mixture
US20100203572A1 (en) * 2007-06-13 2010-08-12 Attomol Gmbh Molekulare Diagnostika Method for carrying out and evaluating mix & measure assays for the measurement of reaction kinetics, concentrations and affinities of analytes in multiplex format
US20110105366A1 (en) * 2007-06-18 2011-05-05 Illumina, Inc. Microfabrication methods for the optimal patterning of substrates
US20090029347A1 (en) * 2007-07-27 2009-01-29 Thornthwaite Jerry T Method for Identifying Multiple Analytes Using Flow Cytometry
EP2201374B1 (en) 2007-08-30 2015-10-07 Trustees Of Tufts College Methods for determining the concentration of an analyte in solution.
US9274056B2 (en) * 2007-12-03 2016-03-01 Robert Hudak Use of non-chelated fluorochromes in rapid test systems
US7843561B2 (en) * 2007-12-17 2010-11-30 Accuri Cytometers, Inc. Optical system for a flow cytometer with an interrogation zone
US8432541B2 (en) 2007-12-17 2013-04-30 Accuri Cytometers, Inc. Optical system for a flow cytometer with an interrogation zone
US20130020507A1 (en) * 2010-06-17 2013-01-24 Life Technologies Corporation Methods for Detecting Defects in Inorganic-Coated Polymer Surfaces
US8202691B2 (en) 2008-01-25 2012-06-19 Illumina, Inc. Uniform fragmentation of DNA using binding proteins
US20090203086A1 (en) * 2008-02-06 2009-08-13 454 Life Sciences Corporation System and method for improved signal detection in nucleic acid sequencing
US8039817B2 (en) 2008-05-05 2011-10-18 Illumina, Inc. Compensator for multiple surface imaging
US20110105356A1 (en) * 2008-05-07 2011-05-05 Derosier Chad F Compositions and methods for providing substances to and from an array
US8470164B2 (en) 2008-06-25 2013-06-25 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
WO2010003132A1 (en) 2008-07-02 2010-01-07 Illumina Cambridge Ltd. Using populations of beads for the fabrication of arrays on surfaces
US8222047B2 (en) 2008-09-23 2012-07-17 Quanterix Corporation Ultra-sensitive detection of molecules on single molecule arrays
US20100075439A1 (en) * 2008-09-23 2010-03-25 Quanterix Corporation Ultra-sensitive detection of molecules by capture-and-release using reducing agents followed by quantification
US20100075862A1 (en) * 2008-09-23 2010-03-25 Quanterix Corporation High sensitivity determination of the concentration of analyte molecules or particles in a fluid sample
US20100087325A1 (en) * 2008-10-07 2010-04-08 Illumina, Inc. Biological sample temperature control system and method
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100137143A1 (en) * 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
WO2010068702A2 (en) * 2008-12-10 2010-06-17 Illumina, Inc. Methods and compositions for hybridizing nucleic acids
FI20095501A0 (en) * 2009-05-04 2009-05-04 Pekka Haenninen Procedure for characterization and / or determination of samples
US20120261274A1 (en) 2009-05-29 2012-10-18 Life Technologies Corporation Methods and apparatus for measuring analytes
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
US8574835B2 (en) 2009-05-29 2013-11-05 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
US8507279B2 (en) 2009-06-02 2013-08-13 Accuri Cytometers, Inc. System and method of verification of a prepared sample for a flow cytometer
US20100330686A1 (en) * 2009-06-29 2010-12-30 Seung Bum Park Nanosensor for sugar detection
GB0913258D0 (en) 2009-07-29 2009-09-02 Dynex Technologies Inc Reagent dispenser
US9523701B2 (en) 2009-07-29 2016-12-20 Dynex Technologies, Inc. Sample plate systems and methods
ES2596323T3 (en) * 2009-11-17 2017-01-05 Asahi Kasei Kabushiki Kaisha Colored organic microparticles and diagnostic reagent kit containing the same
US9073033B2 (en) 2010-01-19 2015-07-07 Illumina, Inc. Methods and compositions for processing chemical reactions
US8422031B2 (en) 2010-02-01 2013-04-16 Illumina, Inc. Focusing methods and optical systems and assemblies using the same
WO2011106402A1 (en) 2010-02-23 2011-09-01 Accuri Cytometers, Inc. Method and system for detecting fluorochromes in a flow cytometer
US8415171B2 (en) 2010-03-01 2013-04-09 Quanterix Corporation Methods and systems for extending dynamic range in assays for the detection of molecules or particles
US8236574B2 (en) 2010-03-01 2012-08-07 Quanterix Corporation Ultra-sensitive detection of molecules or particles using beads or other capture objects
US9678068B2 (en) 2010-03-01 2017-06-13 Quanterix Corporation Ultra-sensitive detection of molecules using dual detection methods
CN103026232B (en) 2010-03-01 2015-02-04 匡特里克斯公司 Methods and systems for extending dynamic range in assays for the detection of molecules or particles
WO2011112465A1 (en) 2010-03-06 2011-09-15 Illumina, Inc. Systems, methods, and apparatuses for detecting optical signals from a sample
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
CA2794522C (en) 2010-04-05 2019-11-26 Prognosys Biosciences, Inc. Spatially encoded biological assays
US9551600B2 (en) 2010-06-14 2017-01-24 Accuri Cytometers, Inc. System and method for creating a flow cytometer network
WO2011159942A1 (en) 2010-06-18 2011-12-22 Illumina, Inc. Conformational probes and methods for sequencing nucleic acids
CN109449171A (en) 2010-06-30 2019-03-08 生命科技公司 For detecting and measuring the transistor circuit of chemical reaction and compound
US8858782B2 (en) 2010-06-30 2014-10-14 Life Technologies Corporation Ion-sensing charge-accumulation circuits and methods
JP5952813B2 (en) 2010-06-30 2016-07-13 ライフ テクノロジーズ コーポレーション Method and apparatus for testing ISFET arrays
US11307166B2 (en) 2010-07-01 2022-04-19 Life Technologies Corporation Column ADC
WO2012006222A1 (en) 2010-07-03 2012-01-12 Life Technologies Corporation Chemically sensitive sensor with lightly doped drains
JP5893564B2 (en) 2010-09-10 2016-03-23 オリンパス株式会社 Optical analysis method using optical measurement in multiple wavelength bands
WO2012032981A1 (en) 2010-09-10 2012-03-15 オリンパス株式会社 Optical analysis method using optical intensity of single light-emitting particle
WO2012036679A1 (en) 2010-09-15 2012-03-22 Life Technologies Corporation Methods and apparatus for measuring analytes
US8796036B2 (en) 2010-09-24 2014-08-05 Life Technologies Corporation Method and system for delta double sampling
US8759038B2 (en) 2010-09-29 2014-06-24 Illumina Cambridge Limited Compositions and methods for sequencing nucleic acids
EP2625526B1 (en) 2010-10-04 2017-03-15 Genapsys Inc. Systems and methods for automated reusable parallel biological reactions
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
ES2897531T3 (en) 2010-10-25 2022-03-01 Accuri Cytometers Inc Systems and user interface for collecting a data set in a flow cytometer
EP2633069B1 (en) 2010-10-26 2015-07-01 Illumina, Inc. Sequencing methods
JP5945506B2 (en) 2010-11-25 2016-07-05 オリンパス株式会社 Optical analysis apparatus and optical analysis method using wavelength characteristics of light of single luminescent particles
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
US9952237B2 (en) 2011-01-28 2018-04-24 Quanterix Corporation Systems, devices, and methods for ultra-sensitive detection of molecules or particles
US9252175B2 (en) 2011-03-23 2016-02-02 Nanohmics, Inc. Method for assembly of spectroscopic filter arrays using biomolecules
US9828696B2 (en) 2011-03-23 2017-11-28 Nanohmics, Inc. Method for assembly of analyte filter arrays using biomolecules
WO2012142301A2 (en) 2011-04-12 2012-10-18 Quanterix Corporation Methods of determining a treatment protocol for and/or a prognosis of a patients recovery from a brain injury
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
US9040307B2 (en) 2011-05-27 2015-05-26 Blood Cell Storage, Inc. Fluorescent pH detector system and related methods
WO2012170936A2 (en) 2011-06-09 2012-12-13 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
EP2735618B1 (en) 2011-07-19 2017-12-06 Hitachi High-Technologies Corporation Nucleic acid analysis method
US8778849B2 (en) 2011-10-28 2014-07-15 Illumina, Inc. Microarray fabrication system and method
US9970984B2 (en) 2011-12-01 2018-05-15 Life Technologies Corporation Method and apparatus for identifying defects in a chemical sensor array
EP2785868B1 (en) 2011-12-01 2017-04-12 Genapsys Inc. Systems and methods for high efficiency electronic sequencing and detection
WO2013096661A1 (en) 2011-12-22 2013-06-27 Illumina, Inc. Methylation biomarkers for ovarian cancer
US8747748B2 (en) 2012-01-19 2014-06-10 Life Technologies Corporation Chemical sensor with conductive cup-shaped sensor surface
US8821798B2 (en) 2012-01-19 2014-09-02 Life Technologies Corporation Titanium nitride as sensing layer for microwell structure
JP6019415B2 (en) * 2012-03-05 2016-11-02 パナソニックＩｐマネジメント株式会社 Sensor device
MX337140B (en) 2012-04-03 2016-02-12 Illumina Inc Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing.
US8906320B1 (en) 2012-04-16 2014-12-09 Illumina, Inc. Biosensors for biological or chemical analysis and systems and methods for same
US8786331B2 (en) 2012-05-29 2014-07-22 Life Technologies Corporation System for reducing noise in a chemical sensor array
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
JP6037701B2 (en) 2012-08-03 2016-12-07 株式会社日立ハイテクノロジーズ Immune analyzer
CA2881685C (en) 2012-08-14 2023-12-05 10X Genomics, Inc. Microcapsule compositions and methods
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9388465B2 (en) 2013-02-08 2016-07-12 10X Genomics, Inc. Polynucleotide barcode generation
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9567631B2 (en) 2012-12-14 2017-02-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
GB201219487D0 (en) 2012-10-30 2012-12-12 Cancer Rec Tech Ltd Anti-S100A4 antibody molecules and their uses
GB201220924D0 (en) 2012-11-21 2013-01-02 Cancer Res Inst Royal Materials and methods for determining susceptibility or predisposition to cancer
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9080968B2 (en) 2013-01-04 2015-07-14 Life Technologies Corporation Methods and systems for point of use removal of sacrificial material
US9841398B2 (en) 2013-01-08 2017-12-12 Life Technologies Corporation Methods for manufacturing well structures for low-noise chemical sensors
US9932626B2 (en) 2013-01-15 2018-04-03 Quanterix Corporation Detection of DNA or RNA using single molecule arrays and other techniques
US8962366B2 (en) 2013-01-28 2015-02-24 Life Technologies Corporation Self-aligned well structures for low-noise chemical sensors
US8963216B2 (en) 2013-03-13 2015-02-24 Life Technologies Corporation Chemical sensor with sidewall spacer sensor surface
PL2969479T3 (en) 2013-03-13 2021-12-27 Illumina, Inc. Multilayer fluidic devices and methods for their fabrication
US8841217B1 (en) 2013-03-13 2014-09-23 Life Technologies Corporation Chemical sensor with protruded sensor surface
US9835585B2 (en) 2013-03-15 2017-12-05 Life Technologies Corporation Chemical sensor with protruded sensor surface
US9809852B2 (en) 2013-03-15 2017-11-07 Genapsys, Inc. Systems and methods for biological analysis
WO2014149779A1 (en) 2013-03-15 2014-09-25 Life Technologies Corporation Chemical device with thin conductive element
US20140264472A1 (en) 2013-03-15 2014-09-18 Life Technologies Corporation Chemical sensor with consistent sensor surface areas
US9116117B2 (en) 2013-03-15 2015-08-25 Life Technologies Corporation Chemical sensor with sidewall sensor surface
JP6581074B2 (en) 2013-03-15 2019-09-25 ライフ テクノロジーズ コーポレーション Chemical sensor with consistent sensor surface area
ES2751402T3 (en) 2013-03-27 2020-03-31 Bluegnome Ltd Aneuploidy risk assessment
US20140336063A1 (en) 2013-05-09 2014-11-13 Life Technologies Corporation Windowed Sequencing
US10458942B2 (en) 2013-06-10 2019-10-29 Life Technologies Corporation Chemical sensor array having multiple sensors per well
US9879313B2 (en) 2013-06-25 2018-01-30 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
ES2719579T3 (en) 2013-07-03 2019-07-11 Illumina Inc System for sequencing by orthogonal synthesis
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9352315B2 (en) 2013-09-27 2016-05-31 Taiwan Semiconductor Manufacturing Company, Ltd. Method to produce chemical pattern in micro-fluidic structure
US10540783B2 (en) 2013-11-01 2020-01-21 Illumina, Inc. Image analysis useful for patterned objects
JP6201856B2 (en) * 2013-11-29 2017-09-27 株式会社Ｊｖｃケンウッド Analysis substrate and method for manufacturing analysis substrate
WO2015089238A1 (en) 2013-12-11 2015-06-18 Genapsys, Inc. Systems and methods for biological analysis and computation
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
EP3094747B1 (en) 2014-01-16 2018-11-07 Illumina, Inc. Gene expression panel for prognosis of prostate cancer recurrence
US9828284B2 (en) 2014-03-28 2017-11-28 Ut-Battelle, Llc Thermal history-based etching
AU2015243445B2 (en) 2014-04-10 2020-05-28 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
EP3556864B1 (en) 2014-04-18 2020-12-09 Genapsys, Inc. Methods and systems for nucleic acid amplification
EP4053292A1 (en) 2014-06-26 2022-09-07 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
KR20170023979A (en) 2014-06-26 2017-03-06 10엑스 제노믹스, 인크. Processes and systems for nucleic acid sequence assembly
WO2016069939A1 (en) 2014-10-29 2016-05-06 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
PT3234187T (en) 2014-12-15 2021-05-26 Illumina Inc Compositions and methods for single molecular placement on a substrate
CN111505087A (en) 2014-12-18 2020-08-07 生命科技公司 Method and apparatus for measuring analytes using large scale FET arrays
US10077472B2 (en) 2014-12-18 2018-09-18 Life Technologies Corporation High data rate integrated circuit with power management
WO2016100486A1 (en) 2014-12-18 2016-06-23 Life Technologies Corporation High data rate integrated circuit with transmitter configuration
CA2972969A1 (en) 2015-01-12 2016-07-21 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
EP3245605B1 (en) 2015-01-13 2022-04-20 10X Genomics, Inc. Systems and methods for visualizing structural variation and phasing information
WO2016130578A1 (en) 2015-02-09 2016-08-18 10X Genomics, Inc. Systems and methods for determining structural variation and phasing using variant call data
EP3262407B1 (en) 2015-02-24 2023-08-30 10X Genomics, Inc. Partition processing methods and systems
WO2016138148A1 (en) 2015-02-24 2016-09-01 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
WO2016154193A1 (en) 2015-03-24 2016-09-29 Illumina, Inc. Methods, carrier assemblies, and systems for imaging samples for biological or chemical analysis
EP4321627A3 (en) 2015-04-10 2024-04-17 10x Genomics Sweden AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
EP3760737B1 (en) 2015-05-11 2023-02-15 Illumina, Inc. Platform for discovery and analysis of therapeutic agents
US10174363B2 (en) 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
RU2699612C2 (en) 2015-05-29 2019-09-06 Иллюмина, Инк. Sample cassette and analytical system for carrying out certain reactions
BR112017023418A2 (en) 2015-07-30 2018-07-24 Illumina, Inc. orthogonal nucleotide unlocking
WO2017034868A1 (en) 2015-08-24 2017-03-02 Illumina, Inc. In-line pressure accumulator and flow-control system for biological or chemical assays
WO2017112025A2 (en) * 2015-10-05 2017-06-29 The University Of North Carolina At Chapel Hill Decoding methods for multiplexing assays and associated fluidic devices, kits, and solid supports
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
CN115369161A (en) 2015-12-04 2022-11-22 10X 基因组学有限公司 Methods and compositions for nucleic acid analysis
US10386351B2 (en) 2015-12-07 2019-08-20 Nanohmics, Inc. Methods for detecting and quantifying analytes using gas species diffusion
US10386365B2 (en) 2015-12-07 2019-08-20 Nanohmics, Inc. Methods for detecting and quantifying analytes using ionic species diffusion
CN109072300B (en) 2015-12-17 2023-01-31 伊路敏纳公司 Differentiating methylation levels in complex biological samples
WO2017123533A1 (en) 2016-01-11 2017-07-20 Illumina, Inc. Detection apparatus having a microfluorometer, a fluidic system, and a flow cell latch clamp module
JP6735348B2 (en) 2016-02-11 2020-08-05 １０エックス ジェノミクス， インコーポレイテッド Systems, methods and media for de novo assembly of whole genome sequence data
KR20180111999A (en) 2016-02-17 2018-10-11 테서렉트 헬스, 인코포레이티드 Sensors and devices for life-time imaging and detection applications
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
DK3377226T3 (en) 2016-03-28 2021-04-26 Illumina Inc Micro rows in several levels
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10544456B2 (en) 2016-07-20 2020-01-28 Genapsys, Inc. Systems and methods for nucleic acid sequencing
WO2018064116A1 (en) 2016-09-28 2018-04-05 Illumina, Inc. Methods and systems for data compression
ES2937782T3 (en) 2016-10-03 2023-03-31 Illumina Inc Fluorescent detection of amines and hydrazines and methods of testing the same
GB201619458D0 (en) 2016-11-17 2017-01-04 Spatial Transcriptomics Ab Method for spatial tagging and analysing nucleic acids in a biological specimen
US20210072255A1 (en) 2016-12-16 2021-03-11 The Brigham And Women's Hospital, Inc. System and method for protein corona sensor array for early detection of diseases
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018119347A1 (en) 2016-12-22 2018-06-28 Quantum-Si Incorporated Integrated photodetector with direct binning pixel
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
WO2018136117A1 (en) 2017-01-20 2018-07-26 Omniome, Inc. Allele-specific capture of nucleic acids
AU2017394645B2 (en) 2017-01-20 2020-01-23 Pacific Biosciences Of California, Inc. Genotyping by polymerase binding
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
GB201701686D0 (en) 2017-02-01 2017-03-15 Illunina Inc System & method with fiducials having offset layouts
GB201701689D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials of non-closed shapes
GB201701688D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials in non-recliner layouts
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
US11492666B2 (en) 2017-02-15 2022-11-08 Pacific Biosciences Of California, Inc. Distinguishing sequences by detecting polymerase dissociation
US10241022B2 (en) * 2017-03-30 2019-03-26 Intel Corporation Characterizing a fluid sample based on response of a non-planar structure
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
EP3625715A4 (en) 2017-05-19 2021-03-17 10X Genomics, Inc. Systems and methods for analyzing datasets
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
SG11201901822QA (en) 2017-05-26 2019-03-28 10X Genomics Inc Single cell analysis of transposase accessible chromatin
CA3072136A1 (en) 2017-08-15 2019-02-21 Omniome, Inc. Scanning apparatus and methods useful for detection of chemical and biological analytes
SG11202002516WA (en) 2017-09-21 2020-04-29 Genapsys Inc Systems and methods for nucleic acid sequencing
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. Methods and systems for nuclecic acid preparation and chromatin analysis
CN114525273A (en) 2017-10-27 2022-05-24 10X基因组学有限公司 Methods and systems for sample preparation and analysis
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
MX2019015838A (en) 2017-12-26 2020-08-03 Illumina Inc Sensor system.
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
EP3775271A1 (en) 2018-04-06 2021-02-17 10X Genomics, Inc. Systems and methods for quality control in single cell processing
EP3782158A1 (en) 2018-04-19 2021-02-24 Omniome, Inc. Improving accuracy of base calls in nucleic acid sequencing methods
CN112567047A (en) 2018-04-26 2021-03-26 欧姆尼欧美公司 Methods and compositions for stabilizing nucleic acid-nucleotide-polymerase complexes
US11339428B2 (en) 2018-05-31 2022-05-24 Pacific Biosciences Of California, Inc. Increased signal to noise in nucleic acid sequencing
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
MX2020013788A (en) 2018-06-22 2021-03-02 Quantum Si Inc Integrated photodetector with charge storage bin of varied detection time.
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
WO2020000959A1 (en) * 2018-06-29 2020-01-02 北京华科泰生物技术股份有限公司 Electrochemical detection method based on marking with markers
AU2019312152A1 (en) 2018-07-24 2021-02-18 Pacific Biosciences Of California, Inc. Serial formation of ternary complex species
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
CN113366117A (en) 2018-08-28 2021-09-07 10X基因组学股份有限公司 Methods for transposase-mediated spatial labeling and analysis of genomic DNA in biological samples
US20210317524A1 (en) 2018-08-28 2021-10-14 10X Genomics, Inc. Resolving spatial arrays
US10731141B2 (en) 2018-09-17 2020-08-04 Omniome, Inc. Engineered polymerases for improved sequencing
GB2578528B (en) 2018-12-04 2021-02-24 Omniome Inc Mixed-phase fluids for nucleic acid sequencing and other analytical assays
CA3103736A1 (en) 2018-12-05 2020-06-11 Illumina Cambridge Limited Methods and compositions for cluster generation by bridge amplification
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
WO2020123309A1 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Resolving spatial arrays by proximity-based deconvolution
GB201820341D0 (en) 2018-12-13 2019-01-30 10X Genomics Inc Method for transposase-mediated spatial tagging and analysing genomic DNA in a biological specimen
GB201820300D0 (en) 2018-12-13 2019-01-30 10X Genomics Inc Method for spatial tagging and analysing genomic DNA in a biological specimen
CA3103633A1 (en) 2018-12-18 2020-06-25 Illumina Cambridge Limited Methods and compositions for paired end sequencing using a single surface primer
PL3899037T3 (en) 2018-12-19 2024-04-08 Illumina, Inc. Methods for improving polynucleotide cluster clonality priority
EP3899032A2 (en) 2018-12-20 2021-10-27 Omniome, Inc. Temperature control for analysis of nucleic acids and other analytes
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
SG11202108788TA (en) 2019-02-12 2021-09-29 10X Genomics Inc Methods for processing nucleic acid molecules
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
EP3924513B1 (en) 2019-02-14 2023-04-12 Pacific Biosciences of California, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
WO2020172444A1 (en) 2019-02-20 2020-08-27 Omniome, Inc. Scanning apparatus and methods for detecting chemical and biological analytes
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
EP3931354A1 (en) 2019-02-28 2022-01-05 10X Genomics, Inc. Profiling of biological analytes with spatially barcoded oligonucleotide arrays
CN113767178A (en) 2019-03-11 2021-12-07 10X基因组学有限公司 Systems and methods for processing optically labeled beads
EP3937780A4 (en) 2019-03-14 2022-12-07 InSilixa, Inc. Methods and systems for time-gated fluorescent-based detection
CN114127309A (en) 2019-03-15 2022-03-01 10X基因组学有限公司 Method for single cell sequencing using spatial arrays
NL2023311B9 (en) 2019-03-21 2021-03-12 Illumina Inc Artificial intelligence-based generation of sequencing metadata
WO2020191387A1 (en) 2019-03-21 2020-09-24 Illumina, Inc. Artificial intelligence-based base calling
NL2023312B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based base calling
NL2023310B1 (en) 2019-03-21 2020-09-28 Illumina Inc Training data generation for artificial intelligence-based sequencing
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
NL2023314B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based quality scoring
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
WO2020198071A1 (en) 2019-03-22 2020-10-01 10X Genomics, Inc. Three-dimensional spatial analysis
US11593649B2 (en) 2019-05-16 2023-02-28 Illumina, Inc. Base calling using convolutions
US11644406B2 (en) 2019-06-11 2023-05-09 Pacific Biosciences Of California, Inc. Calibrated focus sensing
US11377655B2 (en) 2019-07-16 2022-07-05 Pacific Biosciences Of California, Inc. Synthetic nucleic acids having non-natural structures
US10656368B1 (en) 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
CN114651058B (en) 2019-08-05 2023-07-28 禧尔公司 Systems and methods for sample preparation, data generation, and protein crown analysis
US11180520B2 (en) 2019-09-10 2021-11-23 Omniome, Inc. Reversible modifications of nucleotides
CN117036248A (en) 2019-10-01 2023-11-10 10X基因组学有限公司 System and method for identifying morphological patterns in tissue samples
WO2021076152A1 (en) 2019-10-18 2021-04-22 Omniome, Inc. Methods and compositions for capping nucleic acids
EP4055185A1 (en) 2019-11-08 2022-09-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
US20210139867A1 (en) 2019-11-08 2021-05-13 Omniome, Inc. Engineered polymerases for improved sequencing by binding
AU2020388047A1 (en) 2019-11-18 2022-06-23 10X Genomics, Inc. Systems and methods for tissue classification
AU2020388573A1 (en) 2019-11-21 2022-06-23 10X Genomics, Inc, Spatial analysis of analytes
CA3158891A1 (en) 2019-11-22 2021-05-27 Neil Ira WEISENFELD Systems and methods for spatial analysis of analytes using fiducial alignment
CN114885610A (en) 2019-12-23 2022-08-09 10X基因组学有限公司 Methods for spatial analysis using RNA templated ligation
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US20230054204A1 (en) 2020-02-04 2023-02-23 Pacific Biosciences Of California, Inc. Flow cells and methods for their manufacture and use
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US20210265018A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Knowledge Distillation and Gradient Pruning-Based Compression of Artificial Intelligence-Based Base Caller
US20210262018A1 (en) 2020-02-21 2021-08-26 10X Genomics, Inc. Methods and compositions for integrated in situ spatial assay
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
CN115516104A (en) 2020-03-03 2022-12-23 加利福尼亚太平洋生物科学股份有限公司 Methods and compositions for sequencing double-stranded nucleic acids
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
WO2021216708A1 (en) 2020-04-22 2021-10-28 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
EP4146822A1 (en) 2020-05-05 2023-03-15 Pacific Biosciences of California, Inc. Compositions and methods for modifying polymerase-nucleic acid complexes
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
EP4153775A1 (en) 2020-05-22 2023-03-29 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
EP4025692A2 (en) 2020-06-02 2022-07-13 10X Genomics, Inc. Nucleic acid library methods
CN116249785A (en) 2020-06-02 2023-06-09 10X基因组学有限公司 Space transcriptomics for antigen-receptor
EP4162074B1 (en) 2020-06-08 2024-04-24 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4165207A1 (en) 2020-06-10 2023-04-19 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
WO2021263111A1 (en) 2020-06-25 2021-12-30 10X Genomics, Inc. Spatial analysis of dna methylation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4298244A1 (en) 2021-02-23 2024-01-03 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
WO2022198068A1 (en) 2021-03-18 2022-09-22 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
CA3210451A1 (en) 2021-03-22 2022-09-29 Illumina Cambridge Limited Methods for improving nucleic acid cluster clonality
WO2023003757A1 (en) 2021-07-19 2023-01-26 Illumina Software, Inc. Intensity extraction with interpolation and adaptation for base calling
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
US20230070896A1 (en) 2021-09-09 2023-03-09 Nautilus Biotechnology, Inc. Characterization and localization of protein modifications
WO2023049212A2 (en) 2021-09-22 2023-03-30 Illumina, Inc. State-based base calling
US20230215515A1 (en) 2021-12-23 2023-07-06 Illumina Software, Inc. Facilitating secure execution of external workflows for genomic sequencing diagnostics
WO2023122363A1 (en) 2021-12-23 2023-06-29 Illumina Software, Inc. Dynamic graphical status summaries for nucelotide sequencing
WO2023129764A1 (en) 2021-12-29 2023-07-06 Illumina Software, Inc. Automatically switching variant analysis model versions for genomic analysis applications
WO2023183937A1 (en) 2022-03-25 2023-09-28 Illumina, Inc. Sequence-to-sequence base calling
WO2023196572A1 (en) 2022-04-07 2023-10-12 Illumina Singapore Pte. Ltd. Altered cytidine deaminases and methods of use
WO2023239917A1 (en) 2022-06-09 2023-12-14 Illumina, Inc. Dependence of base calling on flow cell tilt
WO2024046838A1 (en) 2022-09-01 2024-03-07 Evonik Operations Gmbh Multi-species chip to detect dna-methylation
WO2024046840A1 (en) 2022-09-01 2024-03-07 Evonik Operations Gmbh Method of assessing protein production in cho cells
WO2024046839A1 (en) 2022-09-01 2024-03-07 Evonik Operations Gmbh Dna-methylation detection in animal-derived products
WO2024069581A1 (en) 2022-09-30 2024-04-04 Illumina Singapore Pte. Ltd. Helicase-cytidine deaminase complexes and methods of use
WO2024073047A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
WO2024073043A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides

Family Cites Families (97)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4200110A (en) 1977-11-28 1980-04-29 United States Of America Fiber optic pH probe
US4499052A (en) 1982-08-30 1985-02-12 Becton, Dickinson And Company Apparatus for distinguishing multiple subpopulations of cells
US4682895A (en) 1985-08-06 1987-07-28 Texas A&M University Fiber optic probe for quantification of colorimetric reactions
US4824789B1 (en) * 1986-10-10 1996-08-13 Minnesota Mining & Mfg Gas sensor
US4798738A (en) * 1986-10-10 1989-01-17 Cardiovascular Devices, Inc. Micro sensor
US5143853A (en) 1986-06-25 1992-09-01 Trustees Of Tufts College Absorbance modulated fluorescence detection methods and sensors
US5114864A (en) 1986-06-25 1992-05-19 Trustees Of Tufts College Fiber optic sensors, apparatus, and detection methods using fluid erodible controlled release polymers for delivery of reagent formulations
US5254477A (en) 1986-06-25 1993-10-19 Trustees Of Tufts College Flourescence intramolecular energy transfer conjugate compositions and detection methods
US5252494A (en) 1986-06-25 1993-10-12 Trustees Of Tufts College Fiber optic sensors, apparatus, and detection methods using controlled release polymers and reagent formulations held within a polymeric reaction matrix
US4822746A (en) 1986-06-25 1989-04-18 Trustees Of Tufts College Radiative and non-radiative energy transfer and absorbance modulated fluorescence detection methods and sensors
EP0269764B1 (en) 1986-12-01 1991-08-28 Molecular Biosystems, Inc. Method for increasing the sensitivity of nucleic acid hybridization assays
SE458968B (en) 1987-06-16 1989-05-22 Wallac Oy BIOSPECIFIC ANALYTICAL PROCEDURE FOR MULTIPLE ANALYTICS WHICH DO NOT INCLUDE PARTICULAR COATING AND LABELING WITH FLUORESCING LABEL SUBSTANCES
US5132242A (en) 1987-07-15 1992-07-21 Cheung Sau W Fluorescent microspheres and methods of using them
US5194300A (en) 1987-07-15 1993-03-16 Cheung Sau W Methods of making fluorescent microspheres
US4785814A (en) 1987-08-11 1988-11-22 Cordis Corporation Optical probe for measuring pH and oxygen in blood and employing a composite membrane
FR2621393B1 (en) * 1987-10-05 1991-12-13 Toledano Jacques DEVICE FOR IMMUNOENZYMATIC DETECTION OF SUBSTANCES FROM A DROP OF BLOOD OR LIQUID FROM ANY BIOLOGICAL MEDIUM
US5002867A (en) 1988-04-25 1991-03-26 Macevicz Stephen C Nucleic acid sequence determination by multiple mixed oligonucleotide probes
NO164622C (en) 1988-05-11 1990-10-24 Tore Lindmo BINAER IMMUNOMETRIC PARTICLE-BASED METHOD FOR MEASURING SPECIFIC SERUM ANTIGENS USING LIQUID FLOW MICROPHOTOMETRY AND A PREPARED TARGET SET UP THEREOF.
US5575849A (en) * 1988-11-25 1996-11-19 Canon Kabushiki Kaisha Apparatus for producing a substrate having a surface with a plurality of spherical dimples for photoconductive members
EP0392546A3 (en) 1989-04-14 1991-09-11 Ro Institut Za Molekularnu Genetiku I Geneticko Inzenjerstvo Process for determination of a complete or a partial contents of very short sequences in the samples of nucleic acids connected to the discrete particles of microscopic size by hybridization with oligonucleotide probes
US5424186A (en) * 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US5326692B1 (en) 1992-05-13 1996-04-30 Molecular Probes Inc Fluorescent microparticles with controllable enhanced stokes shift
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
EP0478319B1 (en) 1990-09-28 1997-04-02 Kabushiki Kaisha Toshiba Gene detection method
US5474895A (en) 1990-11-14 1995-12-12 Siska Diagnostics Inc. Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor
US5105305A (en) 1991-01-10 1992-04-14 At&T Bell Laboratories Near-field scanning optical microscope using a fluorescent probe
US5320814A (en) 1991-01-25 1994-06-14 Trustees Of Tufts College Fiber optic array sensors, apparatus, and methods for concurrently visualizing and chemically detecting multiple analytes of interest in a fluid sample
US5244813A (en) 1991-01-25 1993-09-14 Trustees Of Tufts College Fiber optic sensor, apparatus, and methods for detecting an organic analyte in a fluid or vapor sample
US5250264A (en) 1991-01-25 1993-10-05 Trustees Of Tufts College Method of making imaging fiber optic sensors to concurrently detect multiple analytes of interest in a fluid sample
US5244636A (en) 1991-01-25 1993-09-14 Trustees Of Tufts College Imaging fiber optic array sensors, apparatus, and methods for concurrently detecting multiple analytes of interest in a fluid sample
US5380489A (en) 1992-02-18 1995-01-10 Eastman Kodak Company Element and method for nucleic acid amplification and detection using adhered probes
AU2348092A (en) 1991-07-16 1993-02-23 Transmed Biotech Incorporated Methods and compositions for simultaneous analysis of multiple analytes
US5639603A (en) 1991-09-18 1997-06-17 Affymax Technologies N.V. Synthesizing and screening molecular diversity
US5516635A (en) 1991-10-15 1996-05-14 Ekins; Roger P. Binding assay employing labelled reagent
US6051380A (en) 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US5888723A (en) 1992-02-18 1999-03-30 Johnson & Johnson Clinical Diagnostics, Inc. Method for nucleic acid amplification and detection using adhered probes
EP0565999A2 (en) * 1992-04-16 1993-10-20 Siemens Aktiengesellschaft Optical coupling device for two groups of waveguides
WO1993025563A1 (en) 1992-06-17 1993-12-23 City Of Hope A method of detecting and discriminating between nucleic acid sequences
JP3311752B2 (en) 1992-07-02 2002-08-05 ソイニ，エルッキ Biospecific multivariable test method
EP0723146B1 (en) 1992-09-14 2004-05-06 Sri International Up-converting reporters for biological and other assays using laser excitation techniques
US5565324A (en) 1992-10-01 1996-10-15 The Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
US5298741A (en) 1993-01-13 1994-03-29 Trustees Of Tufts College Thin film fiber optic sensor array and apparatus for concurrent viewing and chemical sensing of a sample
CA2102884A1 (en) 1993-03-04 1994-09-05 James J. Wynne Dental procedures and apparatus using ultraviolet radiation
US20020197456A1 (en) * 1993-06-30 2002-12-26 Pope Edward J. A. Integrated electro-luminescent biochip
JP3302458B2 (en) * 1993-08-31 2002-07-15 富士通株式会社 Integrated optical device and manufacturing method
WO1995014106A2 (en) 1993-11-17 1995-05-26 Id Biomedical Corporation Cycling probe cleavage detection of nucleic acid sequences
US5494798A (en) 1993-12-09 1996-02-27 Gerdt; David W. Fiber optic evanscent wave sensor for immunoassay
US5496997A (en) 1994-01-03 1996-03-05 Pope; Edward J. A. Sensor incorporating an optical fiber and a solid porous inorganic microsphere
DE69531542T2 (en) 1994-02-07 2004-06-24 Beckman Coulter, Inc., Fullerton LIGASE / POLYMERASE-MEDIATED ANALYSIS OF GENETIC ELEMENTS OF SINGLE-NUCLEOTIDE POLYMORPHISMS AND THEIR USE IN GENETIC ANALYSIS
US5856083A (en) 1994-05-06 1999-01-05 Pharmacopeia, Inc. Lawn assay for compounds that affect enzyme activity or bind to target molecules
AU2990595A (en) 1994-07-26 1996-02-22 Sydney Brenner Multidimensional conduit combinatorial library synthesis device
US5512490A (en) 1994-08-11 1996-04-30 Trustees Of Tufts College Optical sensor, optical sensing apparatus, and methods for detecting an analyte of interest using spectral recognition patterns
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5585069A (en) 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
CA2221454A1 (en) 1995-05-19 1996-11-21 Abbott Laboratories Wide dynamic range nucleic acid detection using an aggregate primer series
US5690894A (en) 1995-05-23 1997-11-25 The Regents Of The University Of California High density array fabrication and readout method for a fiber optic biosensor
US5656241A (en) * 1995-09-07 1997-08-12 Optical Sensors Incorporated Method for manufacturing fiber optic sensors
EP0852004B1 (en) 1995-10-11 2011-01-19 Luminex Corporation Multiplexed analysis of clinical specimens
GB9521159D0 (en) 1995-10-16 1995-12-20 Brint Norman T A weapon
US5633972A (en) 1995-11-29 1997-05-27 Trustees Of Tufts College Superresolution imaging fiber for subwavelength light energy generation and near-field optical microscopy
US5814524A (en) 1995-12-14 1998-09-29 Trustees Of Tufts College Optical sensor apparatus for far-field viewing and making optical analytical measurements at remote locations
EP2573101B1 (en) 1996-02-09 2016-04-20 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US5840256A (en) 1996-04-09 1998-11-24 David Sarnoff Research Center Inc. Plate for reaction system
ES2288760T3 (en) 1996-04-25 2008-01-16 Bioarray Solutions Ltd. ELECTROCINETIC ASSEMBLY CONTROLLED BY LIGHT OF PARTICLES NEXT TO SURFACES.
WO1997045559A1 (en) 1996-05-29 1997-12-04 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US5854684A (en) 1996-09-26 1998-12-29 Sarnoff Corporation Massively parallel detection
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
US5900481A (en) 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
DE19647038B4 (en) * 1996-11-14 2007-02-22 Ferro Gmbh Spherical pigments, process for their preparation and their use
US5928819A (en) * 1996-12-19 1999-07-27 Xerox Corporation Methods to fabricate optical equivalents of fiber optic face plates using reactive liquid crystals and polymers
US6083763A (en) 1996-12-31 2000-07-04 Genometrix Inc. Multiplexed molecular analysis apparatus and method
AP9901660A0 (en) * 1997-02-28 1999-09-30 Burstein Lab Inc Laboratory in a disk.
US6023540A (en) 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
CA2287570C (en) 1997-05-02 2008-10-28 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
US6406845B1 (en) 1997-05-05 2002-06-18 Trustees Of Tuft College Fiber optic biosensor for selectively detecting oligonucleotide species in a mixed fluid sample
JP4294740B2 (en) 1997-05-23 2009-07-15 ソレクサ・インコーポレイテッド System and apparatus for serial processing of analytes
WO1998053093A1 (en) 1997-05-23 1998-11-26 Bioarray Solutions Llc Color-encoding and in-situ interrogation of matrix-coupled chemical compounds
US7115884B1 (en) 1997-10-06 2006-10-03 Trustees Of Tufts College Self-encoding fiber optic sensor
WO1999060170A1 (en) 1998-05-21 1999-11-25 Hyseq, Inc. Linear arrays of immobilized compounds and methods of using same
JP3662850B2 (en) 1998-06-24 2005-06-22 イルミナ インコーポレイテッド Decoding array sensors with microspheres
AU4700699A (en) 1998-06-24 2000-01-10 Glaxo Group Limited Nucleotide detection method
US6908770B1 (en) 1998-07-16 2005-06-21 Board Of Regents, The University Of Texas System Fluid based analysis of multiple analytes by a sensor array
US5998175A (en) 1998-07-24 1999-12-07 Lumigen, Inc. Methods of synthesizing and amplifying polynucleotides by ligation of multiple oligomers
WO2000013004A2 (en) 1998-08-26 2000-03-09 Trustees Of Tufts College Combinatorial polymer synthesis of sensors for polymer-based sensor arrays
US6429027B1 (en) * 1998-12-28 2002-08-06 Illumina, Inc. Composite arrays utilizing microspheres
CA2362054A1 (en) 1999-02-09 2000-08-17 John R. Stuelpnagel Intrabead screening methods and compositions
AU771458B2 (en) 1999-02-09 2004-03-25 Illumina, Inc. Automated information processing in randomly ordered arrays
EP1165839A2 (en) 1999-03-26 2002-01-02 Whitehead Institute For Biomedical Research Universal arrays
EP1196630B2 (en) 1999-04-20 2018-10-17 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US6544732B1 (en) 1999-05-20 2003-04-08 Illumina, Inc. Encoding and decoding of array sensors utilizing nanocrystals
EP1190233B1 (en) 1999-05-20 2006-08-30 Illumina, Inc. Apparatus for retaining and presenting at least one microsphere array to solutions and/or to optical imaging systems
AU7569600A (en) 1999-05-20 2000-12-28 Illumina, Inc. Combinatorial decoding of random nucleic acid arrays
US20020051971A1 (en) 1999-05-21 2002-05-02 John R. Stuelpnagel Use of microfluidic systems in the detection of target analytes using microsphere arrays
WO2001004372A1 (en) 1999-07-12 2001-01-18 Evgeny Vasilievich Kuznetsov Heat-resistant high-temperature steel
NZ517121A (en) 1999-09-13 2004-05-28 Nugen Technologies Inc Methods and compositions for linear isothermal amplification of polynucleotide sequences

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US9863880B2 (en) 2013-11-17 2018-01-09 Quantum-Si Incorporated Optical system and assay chip for probing, detecting and analyzing molecules
US9983135B2 (en) 2013-11-17 2018-05-29 Quantum-Si Incorporated Active-source-pixel, integrated device for rapid analysis of biological and chemical specimens
US10048208B2 (en) 2013-11-17 2018-08-14 Quantum-Si Incorporated Integrated device with external light source for probing detecting and analyzing molecules
US9759658B2 (en) 2014-08-08 2017-09-12 Quantum-Si Incorporated Integrated device for temporal binning of received photons
US9885657B2 (en) 2014-08-08 2018-02-06 Quantum-Si Incorporated Integrated device with external light source for probing detecting and analyzing molecules
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