CA2362042A1 - Rapid assembly casting system for slab gels - Google Patents
Rapid assembly casting system for slab gels Download PDFInfo
- Publication number
- CA2362042A1 CA2362042A1 CA002362042A CA2362042A CA2362042A1 CA 2362042 A1 CA2362042 A1 CA 2362042A1 CA 002362042 A CA002362042 A CA 002362042A CA 2362042 A CA2362042 A CA 2362042A CA 2362042 A1 CA2362042 A1 CA 2362042A1
- Authority
- CA
- Canada
- Prior art keywords
- plates
- slab gels
- casting system
- seal
- rapid assembly
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44743—Introducing samples
Abstract
Flat plates serving as molds and enclosures for slab gels are held together by a clamping frame in which the side edges of the plates slide into facing channels and are clamped by lever-operated cams that compress the channel walls against the plate surfaces. The joined plates are held on a vertical support rack containing a finger-operated spring-loaded notched clamp that presses down on the plates to seal the opening at the bottom edges of the plates against a gasket. A well-forming comb for insertion between the plates contains flexible outwardly angled fingers to seal against the spacers between the plates.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/249,378 US6162342A (en) | 1999-02-12 | 1999-02-12 | Rapid assembly casting system for slab gels |
US09/249,378 | 1999-02-12 | ||
PCT/US2000/003649 WO2000047984A1 (en) | 1999-02-12 | 2000-02-10 | Rapid assembly casting system for slab gels |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2362042A1 true CA2362042A1 (en) | 2000-08-17 |
CA2362042C CA2362042C (en) | 2007-05-01 |
Family
ID=22943219
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002362042A Expired - Fee Related CA2362042C (en) | 1999-02-12 | 2000-02-10 | Rapid assembly casting system for slab gels |
Country Status (7)
Country | Link |
---|---|
US (4) | US6162342A (en) |
EP (3) | EP1722220B1 (en) |
JP (4) | JP3665266B2 (en) |
AU (1) | AU757845B2 (en) |
CA (1) | CA2362042C (en) |
DE (1) | DE60034314T2 (en) |
WO (1) | WO2000047984A1 (en) |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6162342A (en) * | 1999-02-12 | 2000-12-19 | Bio-Rad Laboratories, Inc. | Rapid assembly casting system for slab gels |
US6436262B1 (en) * | 2000-04-24 | 2002-08-20 | Bio-Rad Laboratories, Inc. | Compact cell clamp for slab gel plate assembly |
US6576109B1 (en) * | 2000-09-27 | 2003-06-10 | Wealtec Enterprise Co., Ltd. | Gel tray structure of an electrophoresis trough |
FR2825720B1 (en) * | 2001-06-12 | 2003-10-03 | Yonathan Benech | ELECTROPHORESIS DEVICE AND MOLDING ACCESSORIES FOR SUCH A DEVICE |
US6942775B1 (en) | 2002-05-24 | 2005-09-13 | Owl Separation Systems, Inc. | Vertical electrophoresis system |
US20040032058A1 (en) * | 2002-08-13 | 2004-02-19 | Neeper Robert K. | Systems and methods for casting and handling assay matrices |
WO2004063739A1 (en) * | 2002-12-12 | 2004-07-29 | Yonathan Benech | Electrophoresis device and moulding accessories for one such device |
US6969455B1 (en) | 2003-01-22 | 2005-11-29 | Joel Helfer | Electrophoresis frame for gel slabs with different thickness holder plates |
EP1872117B1 (en) * | 2005-04-15 | 2017-03-29 | Life Technologies Corporation | Expanding cam lock for sealing slab gels in an electrophoresis apparatus |
US7588673B2 (en) * | 2005-09-14 | 2009-09-15 | Bio-Rad Laboratories, Inc. | Electrophoresis cassette with sealing and closure features |
US7731829B2 (en) * | 2006-02-13 | 2010-06-08 | Expedion, Inc. | Electrophoresis gel and method of making same |
TWI305836B (en) * | 2006-03-03 | 2009-02-01 | Tzu Chao Jean | Electrophoresis structure |
CN200968946Y (en) * | 2006-09-01 | 2007-10-31 | 北京市六一仪器厂 | In-situ gum producing device |
JP5206611B2 (en) * | 2009-07-17 | 2013-06-12 | 凸版印刷株式会社 | Gel cassette for electrophoresis |
JP2013502598A (en) | 2009-08-24 | 2013-01-24 | ライフ テクノロジーズ コーポレーション | Electrophoresis gel cassette and comb |
USD794823S1 (en) | 2010-08-24 | 2017-08-15 | Life Technologies Corporation | Electrophoresis tank with a base and cassette inserted in |
US9234874B2 (en) | 2011-02-24 | 2016-01-12 | Bio-Rad Laboratories, Inc. | Dimensional stabilization of slab gel cassettes to prevent distortion caused by swelling gels |
EP2969141A4 (en) | 2013-03-15 | 2016-11-30 | Bio Rad Laboratories | Polyacrylamide gels for rapid casting, blotting, and imaging, with storage stability |
USD733921S1 (en) * | 2013-11-26 | 2015-07-07 | Ge Healthcare Bio-Sciences Corp. | Electrophoresis buffer pad |
KR200480488Y1 (en) * | 2014-04-04 | 2016-05-31 | (주)바이온라이프사이언스 | Multi Comb |
EP3374764B1 (en) * | 2015-11-13 | 2024-01-24 | Life Technologies Corporation | Preparation of electrophoresis gels, and related devices, systems, and methods |
CN107831207B (en) * | 2017-12-07 | 2024-03-26 | 安徽师范大学 | Glue making device and use method thereof |
Family Cites Families (29)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3666665A (en) * | 1970-12-14 | 1972-05-30 | Ibm | Composition of ferroelectric matter |
US4142960A (en) * | 1977-09-01 | 1979-03-06 | Terrance Hahn | Slab gel mold and electrophoresis apparatus |
US4290871A (en) * | 1979-01-03 | 1981-09-22 | Hoefer Scientific Instruments | Vertical gel slab electrophoresis method |
US4574040A (en) * | 1984-04-17 | 1986-03-04 | Bio-Rad Laboratories, Inc. | Apparatus for vertical gel electrophoresis |
US4518476A (en) * | 1984-04-17 | 1985-05-21 | Bio-Rad Laboratories, Inc. | End clamp for gel slab plate assembly |
US4784738A (en) * | 1985-12-12 | 1988-11-15 | Bio-Rad Laboratories, Inc. | Apparatus and method for gel casting |
US4773984A (en) * | 1987-02-02 | 1988-09-27 | Life Technologies, Inc. | Vertical gel slab electrophoresis apparatus |
US4732657A (en) * | 1987-06-22 | 1988-03-22 | Haake-Buchler Instruments, Inc. | Gel-slab plate assembly |
CA1338055C (en) * | 1988-04-28 | 1996-02-13 | Robert Richard Hellman Jr. | Electrophoresis device with near-vertical gel plates |
US4954236A (en) * | 1989-06-28 | 1990-09-04 | Bio-Rad Laboratories, Inc. | Apparatus and method for gel casting and electrophoresis in a single enclosure |
US5112470A (en) * | 1990-12-07 | 1992-05-12 | Stratagene | Electrophoresis apparatus |
JP3145468B2 (en) | 1992-02-27 | 2001-03-12 | マニー株式会社 | Lower die mounting structure in caulking device |
US5284565A (en) * | 1992-12-23 | 1994-02-08 | Bio-Rad Laboratories, Inc. | Sample well insert with wedge-shaped profile for ultra-thin slab gels in electrophoresis |
US5626735A (en) * | 1994-05-26 | 1997-05-06 | Bio-Rad Laboratories, Inc. | Electrophoresis slab gel enclosure for gradient gels |
US5540498A (en) * | 1994-07-29 | 1996-07-30 | Bio-Rad Laboratories, Inc. | Cam-operated gradient former |
JPH08233779A (en) | 1995-02-28 | 1996-09-13 | Shimadzu Corp | Gel electrophoretic device |
JP3245321B2 (en) | 1995-03-20 | 2002-01-15 | 北辰工業株式会社 | Method of cutting long sheet-like object having elasticity and its cutting device |
US5792332A (en) * | 1995-12-21 | 1998-08-11 | Hoefer Pharmacia Biotech, Inc. | Safe electrophoresis unit |
US5656145A (en) * | 1996-05-20 | 1997-08-12 | Bio-Rad Laboratories, Inc. | Needle guide for loading samples into a vertical slab gel |
US5632877A (en) | 1996-05-20 | 1997-05-27 | Bio-Rad Laboratories, Inc. | Rapid assembly electrophoresis cell for slab gels |
US5827418A (en) | 1996-10-11 | 1998-10-27 | Hoefer Pharmacia Biotech, Inc. | Electrophoresis cassette |
AUPO403896A0 (en) * | 1996-12-05 | 1997-01-02 | Forbio Research Pty Ltd | Electrophoretic method and apparatus |
US5843295A (en) * | 1996-12-19 | 1998-12-01 | Pharmacia Biotech | Gel electrophoresis well-forming and loading-guide comb |
JP3259165B2 (en) | 1997-04-28 | 2002-02-25 | 住友重機械工業株式会社 | Processing container with clamping mechanism for substrate to be processed |
EP1032827A1 (en) * | 1997-05-13 | 2000-09-06 | Hoefer Pharmacia Biotech, Inc. | Gel casting and electrophoresis device |
JPH1128572A (en) | 1997-07-09 | 1999-02-02 | Bridgestone Metalpha Kk | Butt welding machine |
JP4338107B2 (en) | 1997-10-24 | 2009-10-07 | 蛇の目ミシン工業株式会社 | Embroidery data creation device |
US6001233A (en) * | 1998-04-13 | 1999-12-14 | Novex | Gel electrophoresis apparatus having CAM-activated clamp and methods of use |
US6162342A (en) * | 1999-02-12 | 2000-12-19 | Bio-Rad Laboratories, Inc. | Rapid assembly casting system for slab gels |
-
1999
- 1999-02-12 US US09/249,378 patent/US6162342A/en not_active Expired - Lifetime
-
2000
- 2000-02-10 EP EP06014686A patent/EP1722220B1/en not_active Expired - Lifetime
- 2000-02-10 JP JP2000598844A patent/JP3665266B2/en not_active Expired - Fee Related
- 2000-02-10 DE DE60034314T patent/DE60034314T2/en not_active Expired - Lifetime
- 2000-02-10 EP EP06019844A patent/EP1731904B1/en not_active Expired - Lifetime
- 2000-02-10 WO PCT/US2000/003649 patent/WO2000047984A1/en active IP Right Grant
- 2000-02-10 AU AU28803/00A patent/AU757845B2/en not_active Ceased
- 2000-02-10 EP EP00907279A patent/EP1153287B1/en not_active Expired - Lifetime
- 2000-02-10 CA CA002362042A patent/CA2362042C/en not_active Expired - Fee Related
- 2000-10-25 US US09/696,537 patent/US6869514B1/en not_active Expired - Lifetime
-
2003
- 2003-12-11 JP JP2003413618A patent/JP3836841B2/en not_active Expired - Fee Related
-
2004
- 2004-01-09 US US10/754,336 patent/US7128819B2/en not_active Expired - Fee Related
- 2004-08-19 JP JP2004239768A patent/JP3878634B2/en not_active Expired - Fee Related
-
2006
- 2006-05-18 JP JP2006139066A patent/JP4347863B2/en not_active Expired - Fee Related
- 2006-06-22 US US11/473,619 patent/US7658827B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DE60034314T2 (en) | 2007-12-20 |
EP1153287B1 (en) | 2007-04-11 |
US20060237317A1 (en) | 2006-10-26 |
US7128819B2 (en) | 2006-10-31 |
CA2362042C (en) | 2007-05-01 |
EP1153287A1 (en) | 2001-11-14 |
EP1722220A2 (en) | 2006-11-15 |
JP2006250951A (en) | 2006-09-21 |
US6162342A (en) | 2000-12-19 |
DE60034314D1 (en) | 2007-05-24 |
AU2880300A (en) | 2000-08-29 |
JP2002536661A (en) | 2002-10-29 |
EP1731904A2 (en) | 2006-12-13 |
US7658827B2 (en) | 2010-02-09 |
AU757845B2 (en) | 2003-03-06 |
JP2004085585A (en) | 2004-03-18 |
JP4347863B2 (en) | 2009-10-21 |
JP3665266B2 (en) | 2005-06-29 |
EP1731904B1 (en) | 2012-04-25 |
EP1731904A3 (en) | 2007-06-27 |
US20040140216A1 (en) | 2004-07-22 |
EP1722220B1 (en) | 2012-02-08 |
JP3836841B2 (en) | 2006-10-25 |
JP3878634B2 (en) | 2007-02-07 |
US6869514B1 (en) | 2005-03-22 |
JP2004347613A (en) | 2004-12-09 |
WO2000047984A1 (en) | 2000-08-17 |
EP1722220A3 (en) | 2009-12-02 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |
Effective date: 20180212 |