CA2385342A1 - Improved electrochemical sensor - Google Patents
Improved electrochemical sensor Download PDFInfo
- Publication number
- CA2385342A1 CA2385342A1 CA002385342A CA2385342A CA2385342A1 CA 2385342 A1 CA2385342 A1 CA 2385342A1 CA 002385342 A CA002385342 A CA 002385342A CA 2385342 A CA2385342 A CA 2385342A CA 2385342 A1 CA2385342 A1 CA 2385342A1
- Authority
- CA
- Canada
- Prior art keywords
- base
- test sample
- fluid test
- electrochemical sensor
- sensor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000012530 fluid Substances 0.000 claims abstract 12
- 239000012491 analyte Substances 0.000 claims abstract 7
- 238000000034 method Methods 0.000 claims 5
- 238000007650 screen-printing Methods 0.000 claims 4
- 239000000463 material Substances 0.000 claims 3
- 238000004891 communication Methods 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 1
- 239000008280 blood Substances 0.000 claims 1
- 210000004369 blood Anatomy 0.000 claims 1
- 239000008103 glucose Substances 0.000 claims 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims 1
- 238000007373 indentation Methods 0.000 claims 1
- 229920000136 polysorbate Polymers 0.000 claims 1
- 238000004080 punching Methods 0.000 claims 1
- 125000006850 spacer group Chemical group 0.000 claims 1
- XLNZEKHULJKQBA-UHFFFAOYSA-N terbufos Chemical compound CCOP(=S)(OCC)SCSC(C)(C)C XLNZEKHULJKQBA-UHFFFAOYSA-N 0.000 claims 1
- 238000011144 upstream manufacturing Methods 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/004—Enzyme electrodes mediator-assisted
Abstract
Disclosed is an improved electrochemical sensor for the detection of an analyte in a fluid test sample. The electro-chemical sensor is of the type in which the fluid test sample is drawn into a capillary space and the improvement to the sensor involves an arrangement where a portion of the sensor's counter electrode is placed on the edge of the capillary space in a relationship to the sensor's working electrode such that if the capillary space is not completely filled there will be generated only a very weak current. When the sensor is con-nected to a properly programmed current detector, the weak current caused by the underfilling of the capillary space will be detected as an error and will notify the user of the sensor that the test should not be continued.
Claims (12)
1. In an electrochemical sensor for detecting the concentra-tion of analyte in a fluid test sample comprising (1) a non-conductive base which provides a flow path for the fluid test sample which base has a front edge as well as a counter elec-trode and a working electrode on its upper surface which are in electrical communication with a detector of electrical cur-rent; (2) a reaction layer on at least the surface of the working electrode comprising an enzyme which reacts with the analyte to produce electrons which are transferred to the working electrode; and (3) a cover which when mated with the base forms a capillary space with an opening for the introduc-tion of fluid test sample thereto which space contains the flow path for the fluid test sample in which the counter and working electrodes are situated so that the major portion of the counter electrode is located downstream of the opening from the working electrode with a sub-element of the reference electrode being upstream from the working electrode so that when electrochemical communication between only the sub-element of the counter electrode and the working electrode takes place, there is insufficient flow of electrical current through the detector to constitute a valid test for the con-centration of analyte in the fluid test sample and the detec-tor is pre-programmed to emit an error signal in the event of such insufficient flow of electrical current, the improvement which comprises placing the sub-element of the counter elec-trode on the upper surface of the base so that it is flush with the front edge of the base.
2. The electrochemical sensor of Claim 1 which is connected to a current detector which is programmed to give an error signal when the current it receives is below a predetermined level.
3. The electrochemical sensor of Claim 1 wherein there is a dielectric layer over the working and counter electrode having openings to expose these electrodes to a reaction layer on top of the dielectric layer and the area of the sub-element of the counter electrode which is exposed is 2% to 7% of the area of the working electrode which is exposed.
4. The electrochemical sensor of Claim 3 wherein the sub-element of the counter electrode is not in contact with the reaction layer.
5. The electrochemical sensor of Claim 1 wherein the sub-element is physically disconnected from the rest of the coun-ter electrode and is provided with its own connector from con-tact with a current detector.
6. The electrochemical sensor of Claim 1 wherein the cover has a concave portion; so that the concave portion forms the capillary space when the cover is mated with the base.
7. The electrochemical sensor of Claim 1 which has a spacer element with a U shaped indentation so that the U shaped in-dentation forms the capillary space when it is inserted be-tween the cover and the base.
8. A method for the electrochemical determination of an ana-lyte in a fluid test sample which comprises connecting the electrochemical sensor of Claim 1 to a current detector which is programmed to detect a current which is weaker than a pre-determined current level and introducing the fluid test sample into the capillary space of the sensor.
9. The method of Claim 8 wherein the current detector is programmed to provide an error signal when the current is weaker than the predetermined current level.
10. The method of Claim 8 wherein the fluid test sample is blood and the analyte is glucose.
11. A method for the production of a base member for an elec-trochemical sensor for detecting the concentration of an ana-lyte in a fluid test sample which comprises the steps of:
a) providing an insulating base material in the form of a sheet from which a plurality of bases for the sen-sor can be punched out to form a base having a front edge;
b) screen printing an electrically conductive pattern on to the insulating base material;
c) screen printing a working electrode and a counter electrode onto the conductive pattern so that the main portion of the counter electrode lies further from the front edge of the base than the working electrode and a sub-element of the counter electrode overlaps the base's front edge; and d) punching the base from the sheet of insulating base material in a pattern such that the sub-element of the counter electrode is flush with the front edge of the base.
a) providing an insulating base material in the form of a sheet from which a plurality of bases for the sen-sor can be punched out to form a base having a front edge;
b) screen printing an electrically conductive pattern on to the insulating base material;
c) screen printing a working electrode and a counter electrode onto the conductive pattern so that the main portion of the counter electrode lies further from the front edge of the base than the working electrode and a sub-element of the counter electrode overlaps the base's front edge; and d) punching the base from the sheet of insulating base material in a pattern such that the sub-element of the counter electrode is flush with the front edge of the base.
12. A method for the production of an electrochemical sensor which comprises the steps of:
a) providing the base member prepared as in Claim 11;
b) screen printing a dielectric layer onto the working and control electrodes in a pattern which leaves predetermined portions of these electrodes exposed;
c) screen printing a reaction layer over the dielectric layer; and d) applying a cover member to the base member to form a capillary space into which the fluid test sample can flow;
wherein steps a, b, c and d are carried out before the base member is punched from the sheet of insulating base member ma-terial.
a) providing the base member prepared as in Claim 11;
b) screen printing a dielectric layer onto the working and control electrodes in a pattern which leaves predetermined portions of these electrodes exposed;
c) screen printing a reaction layer over the dielectric layer; and d) applying a cover member to the base member to form a capillary space into which the fluid test sample can flow;
wherein steps a, b, c and d are carried out before the base member is punched from the sheet of insulating base member ma-terial.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US29200501P | 2001-05-21 | 2001-05-21 | |
US60/292,005 | 2001-05-21 |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2385342A1 true CA2385342A1 (en) | 2002-11-21 |
CA2385342C CA2385342C (en) | 2010-12-21 |
Family
ID=23122780
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2385342A Expired - Fee Related CA2385342C (en) | 2001-05-21 | 2002-05-08 | Improved electrochemical sensor |
Country Status (7)
Country | Link |
---|---|
US (2) | US20020175075A1 (en) |
EP (1) | EP1262769B1 (en) |
JP (1) | JP3612307B2 (en) |
AT (1) | ATE493644T1 (en) |
AU (1) | AU784254B2 (en) |
CA (1) | CA2385342C (en) |
DE (1) | DE60238733D1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114544733A (en) * | 2020-11-25 | 2022-05-27 | 五鼎生物技术股份有限公司 | Biochemical test piece |
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-
2002
- 2002-05-06 AU AU38207/02A patent/AU784254B2/en not_active Ceased
- 2002-05-08 DE DE60238733T patent/DE60238733D1/en not_active Expired - Lifetime
- 2002-05-08 CA CA2385342A patent/CA2385342C/en not_active Expired - Fee Related
- 2002-05-08 EP EP02010110A patent/EP1262769B1/en not_active Expired - Lifetime
- 2002-05-08 AT AT02010110T patent/ATE493644T1/en not_active IP Right Cessation
- 2002-05-10 US US10/142,214 patent/US20020175075A1/en not_active Abandoned
- 2002-05-20 JP JP2002144440A patent/JP3612307B2/en not_active Expired - Lifetime
-
2004
- 2004-11-18 US US10/992,593 patent/US7122110B2/en not_active Expired - Fee Related
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114544733A (en) * | 2020-11-25 | 2022-05-27 | 五鼎生物技术股份有限公司 | Biochemical test piece |
Also Published As
Publication number | Publication date |
---|---|
DE60238733D1 (en) | 2011-02-10 |
JP2003004691A (en) | 2003-01-08 |
ATE493644T1 (en) | 2011-01-15 |
CA2385342C (en) | 2010-12-21 |
AU3820702A (en) | 2002-11-28 |
EP1262769A1 (en) | 2002-12-04 |
US20060037870A1 (en) | 2006-02-23 |
AU784254B2 (en) | 2006-03-02 |
US7122110B2 (en) | 2006-10-17 |
EP1262769B1 (en) | 2010-12-29 |
US20020175075A1 (en) | 2002-11-28 |
JP3612307B2 (en) | 2005-01-19 |
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