CA2421267A1 - Fad4, fad5, fad5-2, and fad6, novel fatty acid desaturase family members and uses thereof - Google Patents

Fad4, fad5, fad5-2, and fad6, novel fatty acid desaturase family members and uses thereof Download PDF

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Publication number
CA2421267A1
CA2421267A1 CA002421267A CA2421267A CA2421267A1 CA 2421267 A1 CA2421267 A1 CA 2421267A1 CA 002421267 A CA002421267 A CA 002421267A CA 2421267 A CA2421267 A CA 2421267A CA 2421267 A1 CA2421267 A1 CA 2421267A1
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Prior art keywords
fad5
family members
fatty acid
fad4
fad6
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CA002421267A
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French (fr)
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CA2421267C (en
Inventor
Xiao Qiu
Haiping Hong
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Bioriginal Food and Science Corp
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Priority to CA2608848A priority Critical patent/CA2608848C/en
Publication of CA2421267A1 publication Critical patent/CA2421267A1/en
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
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    • A23L33/115—Fatty acids or derivatives thereof; Fats or oils
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    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
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    • A61K31/202—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids having three or more double bonds, e.g. linolenic
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12P7/6431—Linoleic acids [18:2[n-6]]
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    • C12P7/6432—Eicosapentaenoic acids [EPA]
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    • C12P7/6434—Docosahexenoic acids [DHA]
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    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
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    • C12Y—ENZYMES
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    • C12Y114/19—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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Abstract

The invention provides isolated nucleic acid molecules which encode novel fatty acid desaturase family members. The invention also provides recombinant expression vectors containing desaturase nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., DHA.
CA002421267A 2000-09-28 2001-09-28 Fad4, fad5, fad5-2, and fad6, novel fatty acid desaturase family members and uses thereof Expired - Fee Related CA2421267C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2608848A CA2608848C (en) 2000-09-28 2001-09-28 Fad4, fad5, fad5-2, and fad6, novel fatty acid desaturase family members and uses thereof

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US23630300P 2000-09-28 2000-09-28
US60/236,303 2000-09-28
US29756201P 2001-06-12 2001-06-12
US60/297,562 2001-06-12
PCT/IB2001/002346 WO2002026946A2 (en) 2000-09-28 2001-09-28 Fad4, fad5, fad5-2, and fad6, fatty acid desaturase family members and uses thereof

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA2608848A Division CA2608848C (en) 2000-09-28 2001-09-28 Fad4, fad5, fad5-2, and fad6, novel fatty acid desaturase family members and uses thereof

Publications (2)

Publication Number Publication Date
CA2421267A1 true CA2421267A1 (en) 2002-04-04
CA2421267C CA2421267C (en) 2009-01-06

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ID=26929655

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CA002421267A Expired - Fee Related CA2421267C (en) 2000-09-28 2001-09-28 Fad4, fad5, fad5-2, and fad6, novel fatty acid desaturase family members and uses thereof

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US (7) US7087432B2 (en)
EP (3) EP2166087B1 (en)
JP (3) JP4071622B2 (en)
CN (3) CN105483142A (en)
AT (2) ATE510908T1 (en)
AU (3) AU2002218447C9 (en)
CA (1) CA2421267C (en)
DE (1) DE60141760D1 (en)
DK (2) DK1911837T3 (en)
ES (2) ES2399806T3 (en)
NO (2) NO331104B1 (en)
PT (2) PT1322752E (en)
WO (1) WO2002026946A2 (en)

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