CA2426580A1 - Mass defect labeling for the determination of oligomer sequences - Google Patents
Mass defect labeling for the determination of oligomer sequences Download PDFInfo
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- CA2426580A1 CA2426580A1 CA002426580A CA2426580A CA2426580A1 CA 2426580 A1 CA2426580 A1 CA 2426580A1 CA 002426580 A CA002426580 A CA 002426580A CA 2426580 A CA2426580 A CA 2426580A CA 2426580 A1 CA2426580 A1 CA 2426580A1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6872—Methods for sequencing involving mass spectrometry
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/004—Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn
- H01J49/0045—Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn characterised by the fragmentation or other specific reaction
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/105831—Protein or peptide standard or control [e.g., hemoglobin, etc.]
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25125—Digestion or removing interfering materials
Abstract
Mass tagging methods are provided that lead to mass spectrometer detection sensitivities and molecular discriminations that are improved over other methods. In particular the methods are useful for discriminating tagged molecules and fragments of molecules from chemical noise in the mass spectrum.
These mass tagging methods are useful for oligomer sequencing, determining the relative abundances of molecules from different samples, and identifying individual molecules or chemical processing steps in combinatorial chemical libraries. The methods provided are useful for the simultaneous analysis of multiple molecules and reaction mixtures by mass spectrometric methods.
These mass tagging methods are useful for oligomer sequencing, determining the relative abundances of molecules from different samples, and identifying individual molecules or chemical processing steps in combinatorial chemical libraries. The methods provided are useful for the simultaneous analysis of multiple molecules and reaction mixtures by mass spectrometric methods.
Claims (50)
1. A method for sequencing a terminal portion of an oligomer, comprising:
(a) contacting said oligomer with a labeling moiety to covalently attach a label to the terminus of the oligomer and form a labeled oligomer, said labeling moiety comprising at least one element having an atomic number from 17 to 77, with the proviso that said element is other than sulfur or phosphorus;
(b) fragmenting said labeled oligomer using an enzymatic, chemolytic or mass spectrometric fragmentation method to produce labeled oligomer fragments;
and (c) analyzing said labeled oligomer fragments using a mass spectrometric fragmentation method to determine the sequence of at least two terminal residues.
(a) contacting said oligomer with a labeling moiety to covalently attach a label to the terminus of the oligomer and form a labeled oligomer, said labeling moiety comprising at least one element having an atomic number from 17 to 77, with the proviso that said element is other than sulfur or phosphorus;
(b) fragmenting said labeled oligomer using an enzymatic, chemolytic or mass spectrometric fragmentation method to produce labeled oligomer fragments;
and (c) analyzing said labeled oligomer fragments using a mass spectrometric fragmentation method to determine the sequence of at least two terminal residues.
2. The method of claim 1, wherein said labeling moiety comprises at least one element of atomic number 35 to 63.
3. The method of claim 2, wherein said labeling moiety comprises at least one element of atomic number 39 to 58.
4. The method of claim 2, wherein said labeling moiety comprises at least one element selected from the group consisting of bromine, iodine, europium and yttrium.
5. The method of claim 4, wherein said element is europium.
6. The method of claim 4, wherein said element is yttrium.
7. The method of claim 4, wherein said element is bromine.
8. The method of claim 4, wherein said element is iodine.
9. The method of claim 1, wherein said oligomer is selected from the group consisting of a protein, an oligonucleotide, an oligosaccharide and a lipid.
10. The method of claim 9, wherein said oligomer is an oligonucleotide.
11. The method of claim 9, wherein said sequence is at least three residues.
12. The method of claim 9, wherein said sequence is at least four residues.
13. The method of claim 1, wherein several oligomers, each labeled with a different number of mass defect elements are mixed prior to said fragmenting or analyzing step.
14. A method for sequencing a portion of an oligomer in an oligomer mixture, said method comprising:
(a) contacting said oligomer mixture with a terminus labeling moiety to covalently attach a label to the terminus of said oligomer and form a labeled oligomer mixture, said terminus labeling moiety comprising at least one element having an atomic number from 17 to 77, with the proviso that said element is other than sulfur or phosphorus;
(b) separating individual labeled oligomers in said labeled oligomer mixture;
and (c) analyzing said individual labeled oligomers from step (b) by a mass spectrometric method to determine the sequence of at least two terminus residues.
(a) contacting said oligomer mixture with a terminus labeling moiety to covalently attach a label to the terminus of said oligomer and form a labeled oligomer mixture, said terminus labeling moiety comprising at least one element having an atomic number from 17 to 77, with the proviso that said element is other than sulfur or phosphorus;
(b) separating individual labeled oligomers in said labeled oligomer mixture;
and (c) analyzing said individual labeled oligomers from step (b) by a mass spectrometric method to determine the sequence of at least two terminus residues.
15. A method in accordance with claim 14, wherein said element has an atomic number of from 35 to 63.
16. A method in accordance with claim 14, wherein said element has an atomic number of from 39 to 58.
17. A method in accordance with claim 14, wherein said element is selected from the group consisting of bromine, iodine, europium and yttrium.
18. A method in accordance with claim 14, further comprising a step prior to step (a) of isolating a group of oligomers from a biological sample.
19. A method in accordance with claim 18, wherein said biological sample is from a diseased tissue sample.
20. A method in accordance with claim 18, wherein said biological sample is from a healthy tissue sample.
21. A method in accordance with claim 14, wherein said separating is conducted by at least one method of capillary electrophoresis of the labeled oligomer mixture.
22. A method in accordance with claim 14, wherein said mass spectrometric method uses ESI-TOF MS.
23. A method for structure and function analysis of an oligomer having a plurality of residues, said method comprising:
(a) contacting said oligomer with a mass defect labeling reagent to differentially label exposed residues and unexposed residues and produce a differentially labeled oligomer, wherein said mass defect labeling reagent comprises at least one element having an atomic number of from 17 to 77 that is other than sulfur or phosphorus;
(b) analyzing said differentially labeled oligomer by a mass spectrometric method to determine sequences of said oligomer that are exposed in the three-dimensional structure and sequences of said oligomer that are unexposed in the three-dimensional structure.
(a) contacting said oligomer with a mass defect labeling reagent to differentially label exposed residues and unexposed residues and produce a differentially labeled oligomer, wherein said mass defect labeling reagent comprises at least one element having an atomic number of from 17 to 77 that is other than sulfur or phosphorus;
(b) analyzing said differentially labeled oligomer by a mass spectrometric method to determine sequences of said oligomer that are exposed in the three-dimensional structure and sequences of said oligomer that are unexposed in the three-dimensional structure.
24. A method in accordance with claim 23, wherein said oligomer is a protein, a nucleic acid, or an oligosaccharide.
25. A method in accordance with claim 23, wherein said mass defect labeling reagent comprises at least one element of atomic number 35 to 63.
26. A method in accordance with claim 26, wherein said mass defect labeling reagent is bromine and said oligomer is a protein.
27. A method in accordance with claim 23, wherein said mass defect labeling reagent comprises at least one element of atomic number 39 to 58.
28. A method in accordance with claim 23, wherein said differentially labeled oligomer is fragmented by enzymatic or chemolytic methods prior to step (b).
29. A method in accordance with claim 23, wherein said oligomer is a protein, said mass defect is bromine or iodine and said exposed residues comprises a portion of the tyrosine residues present in said protein.
30. A method in accordance with claim 23, wherein said mass spectrometric method uses ESI-TOF MS.
31. A method in accordance with claim 29, wherein said mass spectrometric method uses ESI-TOF MS.
32. A method for sequencing the terminal portion of an oligomer, comprising:
(a) contacting a first sample of said oligomer with a labeling moiety to covalently attach a label to the terminus of the oligomer and form a labeled oligomer, said labeling moiety having one element with an atomic number from 17 to 77, with the proviso that said element is other than sulfur or phosphorus;
(b) contacting a second sample of said oligomer with a labeling moiety to covalently attach a label to the terminus of the oligomer and form a labeled oligomer, said labeling moiety having two elements with an atomic number from 17 to 77, with the proviso that said elements are other than sulfur or phosphorus;
(c) optionally, repeating step (b) from one to three times with additional samples, wherein the labeling moieties have three, four or five elements, respectively, with an atomic number from 17 to 77, with the proviso that said elements are other than sulfur or phsophorus;
(d) mixing the labeled oligomers from steps (a) through (c);
(e) fragmenting said labeled oligomers using an enzymatic, chemolytic or mass spectrometric fragmentation method to produce labeled oligomer fragments;
and (f) analyzing said labeled oligomer fragments using a mass spectrometric fragmentation method to determine the sequence of at least two terminal residues.
(a) contacting a first sample of said oligomer with a labeling moiety to covalently attach a label to the terminus of the oligomer and form a labeled oligomer, said labeling moiety having one element with an atomic number from 17 to 77, with the proviso that said element is other than sulfur or phosphorus;
(b) contacting a second sample of said oligomer with a labeling moiety to covalently attach a label to the terminus of the oligomer and form a labeled oligomer, said labeling moiety having two elements with an atomic number from 17 to 77, with the proviso that said elements are other than sulfur or phosphorus;
(c) optionally, repeating step (b) from one to three times with additional samples, wherein the labeling moieties have three, four or five elements, respectively, with an atomic number from 17 to 77, with the proviso that said elements are other than sulfur or phsophorus;
(d) mixing the labeled oligomers from steps (a) through (c);
(e) fragmenting said labeled oligomers using an enzymatic, chemolytic or mass spectrometric fragmentation method to produce labeled oligomer fragments;
and (f) analyzing said labeled oligomer fragments using a mass spectrometric fragmentation method to determine the sequence of at least two terminal residues.
33. The method of claim 32, wherein each of said elements has an atomic number of from 35 to 63.
34. The method of claim 32, wherein each of said elements has an atomic number of from 39 to 58.
35. The method of claim 32, wherein each of said elements is selected from the group consisting of bromine, iodine, europium and yttrium and said oligomer is a protein.
36. The method of claim 32, wherein each of said elements is selected from the group consisting of bromine, iodine, europium and yttrium and said oligomer is an oligonucleotide.
37. The method of claim 32, wherein each of said elements is selected from the group consisting of bromine, iodine, europium and yttrium and said oligomer is an oligosaccharide.
38. A method for sequencing a portion of an oligomer, comprising:
(a) fragmenting aliquots of said oligomer using one or more specific enzymatic or chemolytic fragmentation methods to produce oligomer fragments, wherein a different fragmentation method is applied to each aliquot;
(b) contacting a first aliquot of oligomer fragments with a first labeling moiety to covalently attach said first labeling moiety to the terminus of the oligomer fragments and form labeled oligomer fragments, said first labeling moiety having one element with an atomic number from 17 to 77, with the proviso that said element is other than sulfur or phosphorus;
(c) optionally contacting the other aliquots of oligomer fragments with other distinct labeling moieties to covalently attach said distinct labeling moieties to the termini of the oligomer fragments and form labeled oligomer fragments, said distinct labeling moiety having two or more elements with an atomic number from 17 to 77, with the proviso that said elements are other than sulfur or phosphorus;
(d) optionally mixing the aliquots of labeled oligomer fragments; and (e) analyzing said labeled oligomer fragments using a mass spectrometric fragmentation method to determine the sequence of at least two residues of said oligomer.
(a) fragmenting aliquots of said oligomer using one or more specific enzymatic or chemolytic fragmentation methods to produce oligomer fragments, wherein a different fragmentation method is applied to each aliquot;
(b) contacting a first aliquot of oligomer fragments with a first labeling moiety to covalently attach said first labeling moiety to the terminus of the oligomer fragments and form labeled oligomer fragments, said first labeling moiety having one element with an atomic number from 17 to 77, with the proviso that said element is other than sulfur or phosphorus;
(c) optionally contacting the other aliquots of oligomer fragments with other distinct labeling moieties to covalently attach said distinct labeling moieties to the termini of the oligomer fragments and form labeled oligomer fragments, said distinct labeling moiety having two or more elements with an atomic number from 17 to 77, with the proviso that said elements are other than sulfur or phosphorus;
(d) optionally mixing the aliquots of labeled oligomer fragments; and (e) analyzing said labeled oligomer fragments using a mass spectrometric fragmentation method to determine the sequence of at least two residues of said oligomer.
39. A method in accordance with claim 38, wherein said oligomer is a lipid.
40. A method in accordance with claim 38, wherein said oligomer is a protein.
41. A method in accordance with claim 38, wherein said oligomer is a nucleic acid.
42. A method in accordance with claim 38, wherein said oligomer is an oligosaccharide.
43. A method in accordance with claim 38, wherein said elements have an atomic number of from 35 to 63.
44. A method in accordance with claim 43, wherein said elements have an atomic number of from 39 to 58.
45. A method for comparing the relative abundances of analytes from two or more samples, comprising:
(a) contacting the analytes of the first sample with with a labeling moiety to covalently attach a label to the analytes and form labeled analytes, said labeling moiety having one element with an atomic number from 17 to 77, with the proviso that said element is other than sulfur or phosphorus;
(b) contacting the analytes of subsequent samples with labeling moieties to covalently attach labels to the analytes in each sample, wherein the labeling moieties used for each subsequent sample contain an additional element with an atomic number from 17 to 77, with the proviso that said elements are other than sulfur or phosphorus;
(c) mixing the aliquots of labeled analytes; and (d) analyzing said labeled analytes using a mass spectrometric fragmentation method to determine the relative abundances of one or more of the analytes between the samples.
(a) contacting the analytes of the first sample with with a labeling moiety to covalently attach a label to the analytes and form labeled analytes, said labeling moiety having one element with an atomic number from 17 to 77, with the proviso that said element is other than sulfur or phosphorus;
(b) contacting the analytes of subsequent samples with labeling moieties to covalently attach labels to the analytes in each sample, wherein the labeling moieties used for each subsequent sample contain an additional element with an atomic number from 17 to 77, with the proviso that said elements are other than sulfur or phosphorus;
(c) mixing the aliquots of labeled analytes; and (d) analyzing said labeled analytes using a mass spectrometric fragmentation method to determine the relative abundances of one or more of the analytes between the samples.
46. A method in accordance with claim 45, wherein said elements have an atomic number of from 35 to 63.
47. A method in accordance with claim 45, wherein said elements have an atomic number of from 39 to 58.
48. A method for tagging the elements of chemical libraries, either during synthesis or screening, comprising;
(a) contacting a root tag with a labeling moiety to covalently attach a label to the root tag and form a labeled tag, said labeling moiety having one element with an atomic number from 17 to 77, with the proviso that said element is other than sulfur or phosphorus;
(b) optionally, contacting a root with additional labeling moieties to covalently attach additional labels to the root tag and form a multiply labeled tag, said labeling moiety having one element with an atomic number from 17 to 77, with the proviso that said element is other than sulfur or phosphorus; and (c) analyzing the labeled tag by mass spectrometric methods to determine both its mass and the number of elements with an atomic number from 17 to 77, such that the mass and number of elements identifies the chemical processes to which the specific chemical of the library has been exposed and the identity of the chemical from the library.
(a) contacting a root tag with a labeling moiety to covalently attach a label to the root tag and form a labeled tag, said labeling moiety having one element with an atomic number from 17 to 77, with the proviso that said element is other than sulfur or phosphorus;
(b) optionally, contacting a root with additional labeling moieties to covalently attach additional labels to the root tag and form a multiply labeled tag, said labeling moiety having one element with an atomic number from 17 to 77, with the proviso that said element is other than sulfur or phosphorus; and (c) analyzing the labeled tag by mass spectrometric methods to determine both its mass and the number of elements with an atomic number from 17 to 77, such that the mass and number of elements identifies the chemical processes to which the specific chemical of the library has been exposed and the identity of the chemical from the library.
49. A method in accordance with claim 48, wherein said elements have an atomic number of from 35 to 63.
50. A method in accordance with claim 48, wherein said elements have an atomic number of from 39 to 58.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US24216500P | 2000-10-19 | 2000-10-19 | |
| US24239800P | 2000-10-19 | 2000-10-19 | |
| US60/242,165 | 2000-10-19 | ||
| US60/242,398 | 2000-10-19 | ||
| PCT/US2001/049951 WO2002066952A2 (en) | 2000-10-19 | 2001-10-19 | Mass defect labeling for the determination of oligomer sequences |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2426580A1 true CA2426580A1 (en) | 2002-08-29 |
| CA2426580C CA2426580C (en) | 2011-09-13 |
Family
ID=26934884
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2426580A Expired - Fee Related CA2426580C (en) | 2000-10-19 | 2001-10-19 | Mass defect labeling for the determination of oligomer sequences |
| CA000000001A Abandoned CA2425798A1 (en) | 2000-10-19 | 2001-10-19 | Methods for determining protein and peptide terminal sequences |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000000001A Abandoned CA2425798A1 (en) | 2000-10-19 | 2001-10-19 | Methods for determining protein and peptide terminal sequences |
Country Status (13)
| Country | Link |
|---|---|
| US (4) | US6962818B2 (en) |
| EP (3) | EP2479578A1 (en) |
| JP (3) | JP4259869B2 (en) |
| AT (1) | ATE552348T1 (en) |
| AU (2) | AU2002246744B2 (en) |
| CA (2) | CA2426580C (en) |
| DK (1) | DK1358458T3 (en) |
| ES (1) | ES2385335T3 (en) |
| IL (5) | IL155518A0 (en) |
| IS (1) | IS6781A (en) |
| NO (1) | NO20033632D0 (en) |
| PT (1) | PT1358458E (en) |
| WO (2) | WO2002066952A2 (en) |
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