CA2432075A1 - Isolation of nucleic acids - Google Patents
Isolation of nucleic acids Download PDFInfo
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- CA2432075A1 CA2432075A1 CA002432075A CA2432075A CA2432075A1 CA 2432075 A1 CA2432075 A1 CA 2432075A1 CA 002432075 A CA002432075 A CA 002432075A CA 2432075 A CA2432075 A CA 2432075A CA 2432075 A1 CA2432075 A1 CA 2432075A1
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- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
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- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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Abstract
The present invention relates to materials for extracting nucleic acid using charge switch methods which involve, at a first pH, bringing the sample into contact with a material which comprises an ionisable group, wherein the material has a positive charge at said first pH, such that nucleic acid is bound to the material; and releasing the nucleic acid at a second, higher, p H at which the charge on the material is negative, neutral or less positive. T he release of the nucleic acid occurs under mild conditions and the ionisable group is provided by a biological buffer, a polyhydroxylated amine, a detergent or surfactant, a carbohydrate, a nucleic acid base, a heterocyclic nitrogen-containing compound, a monoamine, a biological dye, or a negatively ionisable group, the pKa of which is between about 3.0 and 7.0 and a metal oxide which is positively charged at said first pH, and optionally also at said second pH.
Description
Isolation of Nucleic Acids Field of the Invention The present invention relates to methods for extracting nucleic acids from samples and products useful in carrying out these methods, in particular for extracting nucleic acid from biological samples such as blood.
Background of the Invention There is a very large demand for DNA analysis for a range of purposes and this has lead to the requirement for quick, safe, high throughput methods for the isolation and purification of DNA and other nucleic acids.
Samples for use for DNA identification or analysis can be taken from a wide range of sources such as biological material such as animal and plant cells, faeces, tissue etc. also samples can be taken from soil, foodstuffs, water etc.
Existing methods for the extraction of DNA include the use of phenol/chloroform, salting out, the use of chaotropic salts and silica resins, the use of affinity resins, ion exchange chromatography and the use of magnetic beads. Methods are described in US Patents 5057426, 4923978, EP Patents 0512767 A1 and EP 0515484B
and WO 95/13368, WO 97/10331 and WO 96/18731. These patents and patent applications disclose methods of adsorbing nucleic acids on to a solid support and then isolating the nucleic acids. The previously used methods use some type of solvent to isolate the nucleic acids and these solvents are often flammable, combustible or toxic.
EP0707077A2 describes a synthetic water soluble polymer to precipitate nucleic acids at acid pH and release at alkaline pH. The re-dissolving of the nucleic acids is performed at extremes of pH, temperature and/or high salt concentrations where the nucleic acids, especially RNA, can become denatured, degraded or require further purification or adjustments before storage and analysis.
W096/09116 discloses mixed mode resins for recovering a target compound, especially a protein, from aqueous solution at high or low ionic strength, using changes in pH. The resins have a hydrophobic character at the pH of binding of the target compound and a hydrophilic and/or electrostatic character at the pH of desorption of the target compound.
Blood is one of the most abundant sample sources for DNA
analysis as blood samples are routinely taken for a wide range of reasons. However because of the viscous and proteinaceous nature of blood using known DNA extraction methods it has proved difficult to accomplish using automation due to the difficulties of handling large volumes containing relatively small amounts of DNA.
Hitherto nucleic acid extraction has been partially automated only by using hazardous reagents and slow processing times.
W099/29703 discloses the use of charge switch materials for purifying nucleic acid, binding nucleic acid in a sample to a solid phase at a low pH (e.g. pH 6) and releasing the nucleic acid at a higher pH (e.g. pH 8).
The application exemplifies the use of solid phases incorporating histidine or polyhistidine groups.
Background of the Invention There is a very large demand for DNA analysis for a range of purposes and this has lead to the requirement for quick, safe, high throughput methods for the isolation and purification of DNA and other nucleic acids.
Samples for use for DNA identification or analysis can be taken from a wide range of sources such as biological material such as animal and plant cells, faeces, tissue etc. also samples can be taken from soil, foodstuffs, water etc.
Existing methods for the extraction of DNA include the use of phenol/chloroform, salting out, the use of chaotropic salts and silica resins, the use of affinity resins, ion exchange chromatography and the use of magnetic beads. Methods are described in US Patents 5057426, 4923978, EP Patents 0512767 A1 and EP 0515484B
and WO 95/13368, WO 97/10331 and WO 96/18731. These patents and patent applications disclose methods of adsorbing nucleic acids on to a solid support and then isolating the nucleic acids. The previously used methods use some type of solvent to isolate the nucleic acids and these solvents are often flammable, combustible or toxic.
EP0707077A2 describes a synthetic water soluble polymer to precipitate nucleic acids at acid pH and release at alkaline pH. The re-dissolving of the nucleic acids is performed at extremes of pH, temperature and/or high salt concentrations where the nucleic acids, especially RNA, can become denatured, degraded or require further purification or adjustments before storage and analysis.
W096/09116 discloses mixed mode resins for recovering a target compound, especially a protein, from aqueous solution at high or low ionic strength, using changes in pH. The resins have a hydrophobic character at the pH of binding of the target compound and a hydrophilic and/or electrostatic character at the pH of desorption of the target compound.
Blood is one of the most abundant sample sources for DNA
analysis as blood samples are routinely taken for a wide range of reasons. However because of the viscous and proteinaceous nature of blood using known DNA extraction methods it has proved difficult to accomplish using automation due to the difficulties of handling large volumes containing relatively small amounts of DNA.
Hitherto nucleic acid extraction has been partially automated only by using hazardous reagents and slow processing times.
W099/29703 discloses the use of charge switch materials for purifying nucleic acid, binding nucleic acid in a sample to a solid phase at a low pH (e.g. pH 6) and releasing the nucleic acid at a higher pH (e.g. pH 8).
The application exemplifies the use of solid phases incorporating histidine or polyhistidine groups.
Summary of the Invention Broadly, the present invention relates to novel materials and conditions for extracting or purifying nucleic acid that continue and improve over the initial work disclosed in W099/29703.
I have now devised an improved method for the extraction of nucleic acids and other biomolecules from blood and other biological materials, and other samples containing nucleic acid.
According to the invention there is provided a method for the extraction of biomolecules from biological material which method comprises contacting the biological material with a solid phase which is able to bind the biomolecules to it at a first pH and then extracting the biomolecules bound to the solid phase by elution using an elution solvent at a second pH.
Tn particular there is provided a method for extracting nucleic acid from a sample containing nucleic acid, which method comprises: contacting the sample with said solid phase at a first pH at which the solid phase has a positive charge and will bind negatively charged nucleic acid;~and then releasing the nucleic acid at a higher pH
at which the solid phase possesses a neutral, negative or less positive charge than at the first pH.
Generally the solid phase will possess an overall positive charge, that is the sum of all positive and negative charges on the solid phase as a whole is positive. It is possible (though not preferred), however, that the solid phase as a whole could be negatively charged, but have areas of predominantly positive charge to which the nucleic acid can bind. Such solid phases are within the scope of the invention.
The change in the charge of the solid phase is referred to herein as "charge switching" and is accomplished by the use of a "charge switch material" in, on or as the solid phase.
The charge switch material comprises an ionisable group, which changes charge to according to the ambient conditions. The charge switch material is chosen so that the pKa of the ionisable group is appropriate to the conditions at which it is desired to bind nucleic acid to and release nucleic acid from the solid phase.
Generally, nucleic acid will be bound to the charge switch material at a pH below or roughly equal to the pKa, when the charge switch material is positively charged, and will be released at a higher pH (usually above the pKa), when the charge switch material is less positively charged, neutral, or negatively charged.
The present invention is more particularly directed to the use of charge switch materials which allow binding and/or releasing (especially releasing) of the nucleic acid to occur under mild conditions of temperature and/or pH and/or ionic strength.
Generally the charge switch material will change charge because of a change in charge on a positively ionisable group from positive to less positive or neutral, as the pH is increased in a range spanning or close to the pKa of the positively ionisable group. This may also be combined with a change of charge on a negatively ionisable group from neutral or less negative to more negative. In an alternative embodiment (described below), however, the charge switch material comprises a material which is positively charged at both pH values (such as a metal oxide) and a negatively ionisable group, the charge of which becomes more negative as the pH is increased in a range spanning or close to its pKa.
The charge switch material may comprise an ionisable group having a pKa between about 3 and 9. For positively ionisable groups, the pKa is more preferably at least about 4.5, 5.0, 5.5, 6.0 or 6.5 and/or at most about 8.5, 8.0, 7.5 or 7Ø A particularly preferred pKa for a positively ionisable group is between about 5 and 8; even more preferred is a pKa between about 6.0 and 7.0, more preferably between about 6.5 and 7Ø The pKa for negatively ionisable groups is preferably between about 3 and 7, still more preferably between about 4 and 6, further preferably approximately at the pH at which it is desired to bind nucleic acid.
Materials having more than one pKa value (e. g. having different ionisable groups), or combinations of materials having different pKa values, may also be suitable for use as charge switch materials in accordance with the invention, provided that at a first (lower) pH the materials) possesses) a positive charge and that at a higher pH the charge is less positive, neutral or negative.
Generally a charge switch will be achieved by changing the pH from a value below to a value above the pKa of the or an ionisable group. However, it will be appreciated that when the pH is the same as the pKa value of a particular ionisable group, 500 of the individual ionisable groups will be charged and 50o neutral.
Therefore, charge switch effects can also be achieved by changing the pH in a range close to, but not spanning, the pKa of an ionisable group. For example, at the pICa of a negatively ionisable group, such as a carboxy group (pKa typically around 4), 500 of such groups will be in the ionised form (e. g. C00') and 50% in the neutral form (e. g. COOH). As the pH increases, an increasing proportion of the groups will be in the negative form.
Preferably the binding step is carried out at a pH of below the pKa of the ionisable group, or (though this is not preferred) within about 1 pH unit above the pICa.
Generally the releasing step is carried out at a pH above the pKa of the ionisable group, preferably at a pH
between 1 and 3 pH units above the pKa.
Prior art methods, such as those disclosed in EP0707077, often use high pH to release the nucleic acid, for example using strong bases such as NaOH. Such high pH
can cause depurination of nucleic acid, leading to the problems of imperfect replication, which can impede subsequent use of the nucleic acid, e.g. in detection and/or amplification techniques such as Southern or northern blotting or PCR.
The use of strong bases, or weak bases in combination with heating, again as in EP0707077, can also lead to degradation of RNA (especially at pH values of 10 or above), and denaturation of double stranded DNA (i.e.
irreversible conversion of DNA from the double stranded form at least partially into the single stranded form), which can lead to a lack of specific binding in PCR.
I have now devised an improved method for the extraction of nucleic acids and other biomolecules from blood and other biological materials, and other samples containing nucleic acid.
According to the invention there is provided a method for the extraction of biomolecules from biological material which method comprises contacting the biological material with a solid phase which is able to bind the biomolecules to it at a first pH and then extracting the biomolecules bound to the solid phase by elution using an elution solvent at a second pH.
Tn particular there is provided a method for extracting nucleic acid from a sample containing nucleic acid, which method comprises: contacting the sample with said solid phase at a first pH at which the solid phase has a positive charge and will bind negatively charged nucleic acid;~and then releasing the nucleic acid at a higher pH
at which the solid phase possesses a neutral, negative or less positive charge than at the first pH.
Generally the solid phase will possess an overall positive charge, that is the sum of all positive and negative charges on the solid phase as a whole is positive. It is possible (though not preferred), however, that the solid phase as a whole could be negatively charged, but have areas of predominantly positive charge to which the nucleic acid can bind. Such solid phases are within the scope of the invention.
The change in the charge of the solid phase is referred to herein as "charge switching" and is accomplished by the use of a "charge switch material" in, on or as the solid phase.
The charge switch material comprises an ionisable group, which changes charge to according to the ambient conditions. The charge switch material is chosen so that the pKa of the ionisable group is appropriate to the conditions at which it is desired to bind nucleic acid to and release nucleic acid from the solid phase.
Generally, nucleic acid will be bound to the charge switch material at a pH below or roughly equal to the pKa, when the charge switch material is positively charged, and will be released at a higher pH (usually above the pKa), when the charge switch material is less positively charged, neutral, or negatively charged.
The present invention is more particularly directed to the use of charge switch materials which allow binding and/or releasing (especially releasing) of the nucleic acid to occur under mild conditions of temperature and/or pH and/or ionic strength.
Generally the charge switch material will change charge because of a change in charge on a positively ionisable group from positive to less positive or neutral, as the pH is increased in a range spanning or close to the pKa of the positively ionisable group. This may also be combined with a change of charge on a negatively ionisable group from neutral or less negative to more negative. In an alternative embodiment (described below), however, the charge switch material comprises a material which is positively charged at both pH values (such as a metal oxide) and a negatively ionisable group, the charge of which becomes more negative as the pH is increased in a range spanning or close to its pKa.
The charge switch material may comprise an ionisable group having a pKa between about 3 and 9. For positively ionisable groups, the pKa is more preferably at least about 4.5, 5.0, 5.5, 6.0 or 6.5 and/or at most about 8.5, 8.0, 7.5 or 7Ø A particularly preferred pKa for a positively ionisable group is between about 5 and 8; even more preferred is a pKa between about 6.0 and 7.0, more preferably between about 6.5 and 7Ø The pKa for negatively ionisable groups is preferably between about 3 and 7, still more preferably between about 4 and 6, further preferably approximately at the pH at which it is desired to bind nucleic acid.
Materials having more than one pKa value (e. g. having different ionisable groups), or combinations of materials having different pKa values, may also be suitable for use as charge switch materials in accordance with the invention, provided that at a first (lower) pH the materials) possesses) a positive charge and that at a higher pH the charge is less positive, neutral or negative.
Generally a charge switch will be achieved by changing the pH from a value below to a value above the pKa of the or an ionisable group. However, it will be appreciated that when the pH is the same as the pKa value of a particular ionisable group, 500 of the individual ionisable groups will be charged and 50o neutral.
Therefore, charge switch effects can also be achieved by changing the pH in a range close to, but not spanning, the pKa of an ionisable group. For example, at the pICa of a negatively ionisable group, such as a carboxy group (pKa typically around 4), 500 of such groups will be in the ionised form (e. g. C00') and 50% in the neutral form (e. g. COOH). As the pH increases, an increasing proportion of the groups will be in the negative form.
Preferably the binding step is carried out at a pH of below the pKa of the ionisable group, or (though this is not preferred) within about 1 pH unit above the pICa.
Generally the releasing step is carried out at a pH above the pKa of the ionisable group, preferably at a pH
between 1 and 3 pH units above the pKa.
Prior art methods, such as those disclosed in EP0707077, often use high pH to release the nucleic acid, for example using strong bases such as NaOH. Such high pH
can cause depurination of nucleic acid, leading to the problems of imperfect replication, which can impede subsequent use of the nucleic acid, e.g. in detection and/or amplification techniques such as Southern or northern blotting or PCR.
The use of strong bases, or weak bases in combination with heating, again as in EP0707077, can also lead to degradation of RNA (especially at pH values of 10 or above), and denaturation of double stranded DNA (i.e.
irreversible conversion of DNA from the double stranded form at least partially into the single stranded form), which can lead to a lack of specific binding in PCR.
The appropriate choice of pKa values) in accordance with the invention allows the step of releasing DNA from the solid phase to be performed under mild conditions, unlike in the prior art. As used herein, the term "mild conditions" generally means conditions under which nucleic acid is not denatured and/or not degraded and/or not depurinated, and/or conditions which are substantially physiological.
Preferably the releasing step is performed at a pH of no greater than about pH 10.5, more preferably no greater than about pH 10.0, 9.8, 9.6, 9.4, 9.2, 9.0, 8.9, 8.8, 8.7, 8.6 or 8.5. Depending on the pKa(s) of the charge switch material, the releasing step may even be performed at lower pH values, such as 8.0, 7.5 or 7Ø Preferably the releasing step is carried out in the substantial absence of NaOH, preferably also the substantial absence of other alkali metal hydroxides, more preferably the substantial absence of strong mineral bases. Substantial absence may mean that the concentration is less than 25mM, preferably less than 20mM, more preferably less than l5mM or lOmM.
The desired change in pH can be achieved by altering the ionic.strength of the solution and/or the temperature, since pH is dependent on both these factors. However, neither high temperature nor high ionic strength are generally compatible with the desired mild conditions, and accordingly, the change in pH is preferably not achieved by large changes in ionic strength or temperature. Moreover, increasing ionic strength increases competition of charged species with the nucleic acid for binding to the solid phase, so can assist in releasing the nucleic acid. Small changes of ionic strength are therefore acceptable and may be used in conjunction with the change in pH to release the nucleic acid, preferably within the limits and ranges given below.
Preferably the temperature at which the releasing step performed is no greater than about 70°C, more preferably no greater than about 65°C, 60°C, 55°C, 50°C, 45°C or 40°C. More preferably, such temperatures apply to the entire process. The releasing step, or the entire process, may even be performed at lower temperatures, such as 35°C, 30°C or 25°C.
Furthermore, the releasing step preferably occurs under conditions of low ionic strength, suitably less than 1M
or 500 mM, preferably less than 400mM, 300mM, 200mM, 100mM, 75mM, 50mM, 40mM, 30mM, 25mM, 20mM or lSmM. It may even be below lOmM. The ionic strength may be at least about 5mM, more preferably at least about lOmM.
More preferably, these ionic strengths also apply to the binding step.
PCR is sensitive to pH and the presence of charged contaminants. In particularly preferred embodiments, the releasing step is performed using reagents suitable for storing nucleic acid (such as a commercially available storage buffer, e.g. lOmM Tris.HCl, pH8.0-8.5, optionally in the presence of 1mM EDTA), or using reagents suitable for use in a procedure to which the nucleic acid is to be subjected (such as a PCR buffer, e.g. lOmM Tris.HCl, 50mM
KCl, pH 8.5).
Common previously known nucleic acid extraction processes require a step of diluting the elution product containing nucleic acid, to make the solution suitable for e.g. PCR.
Preferably the present invention substantially avoids diluting the released nucleic acid.
Preferably the step of binding DNA occurs under mild conditions, suitably at a pH of no less than 3.0, preferably no less than 3.5, 4.0, 4.5 or 5Ø Previous methods have used high concentrations of chaotropic agents, such as 8M guanidine. Such conditions may not be necessary in the practice of the present invention, in which the binding step preferably occurs in solution having a total concentration of 1M or less. More preferred temperatures and ionic strengths are as detailed above for the releasing step.
The use of such mild conditions for the release of nucleic acid is especially useful for extracting small quantities of nucleic acid, as the extracted DNA or RNA
can be added directly to a reaction or storage tube without further purification steps (e. g. steps necessitated by the use of high ion concentrations in prior art methods), and without the need to dilute high ionic strength (as is the case with prior art methods using high ionic strength to elute the nucleic acid).
Therefore loss of nucleic acid through changing the container, imperfect recovery during purification steps, degradation, or denaturation, and dilution of small amounts of nucleic acid can be avoided. This is particularly advantageous when a nucleic acid of interest is present in a sample (or is expected to be present) at a low copy number, such as in certain detection and/or amplification methods.
Preferably the releasing step is performed at a pH of no greater than about pH 10.5, more preferably no greater than about pH 10.0, 9.8, 9.6, 9.4, 9.2, 9.0, 8.9, 8.8, 8.7, 8.6 or 8.5. Depending on the pKa(s) of the charge switch material, the releasing step may even be performed at lower pH values, such as 8.0, 7.5 or 7Ø Preferably the releasing step is carried out in the substantial absence of NaOH, preferably also the substantial absence of other alkali metal hydroxides, more preferably the substantial absence of strong mineral bases. Substantial absence may mean that the concentration is less than 25mM, preferably less than 20mM, more preferably less than l5mM or lOmM.
The desired change in pH can be achieved by altering the ionic.strength of the solution and/or the temperature, since pH is dependent on both these factors. However, neither high temperature nor high ionic strength are generally compatible with the desired mild conditions, and accordingly, the change in pH is preferably not achieved by large changes in ionic strength or temperature. Moreover, increasing ionic strength increases competition of charged species with the nucleic acid for binding to the solid phase, so can assist in releasing the nucleic acid. Small changes of ionic strength are therefore acceptable and may be used in conjunction with the change in pH to release the nucleic acid, preferably within the limits and ranges given below.
Preferably the temperature at which the releasing step performed is no greater than about 70°C, more preferably no greater than about 65°C, 60°C, 55°C, 50°C, 45°C or 40°C. More preferably, such temperatures apply to the entire process. The releasing step, or the entire process, may even be performed at lower temperatures, such as 35°C, 30°C or 25°C.
Furthermore, the releasing step preferably occurs under conditions of low ionic strength, suitably less than 1M
or 500 mM, preferably less than 400mM, 300mM, 200mM, 100mM, 75mM, 50mM, 40mM, 30mM, 25mM, 20mM or lSmM. It may even be below lOmM. The ionic strength may be at least about 5mM, more preferably at least about lOmM.
More preferably, these ionic strengths also apply to the binding step.
PCR is sensitive to pH and the presence of charged contaminants. In particularly preferred embodiments, the releasing step is performed using reagents suitable for storing nucleic acid (such as a commercially available storage buffer, e.g. lOmM Tris.HCl, pH8.0-8.5, optionally in the presence of 1mM EDTA), or using reagents suitable for use in a procedure to which the nucleic acid is to be subjected (such as a PCR buffer, e.g. lOmM Tris.HCl, 50mM
KCl, pH 8.5).
Common previously known nucleic acid extraction processes require a step of diluting the elution product containing nucleic acid, to make the solution suitable for e.g. PCR.
Preferably the present invention substantially avoids diluting the released nucleic acid.
Preferably the step of binding DNA occurs under mild conditions, suitably at a pH of no less than 3.0, preferably no less than 3.5, 4.0, 4.5 or 5Ø Previous methods have used high concentrations of chaotropic agents, such as 8M guanidine. Such conditions may not be necessary in the practice of the present invention, in which the binding step preferably occurs in solution having a total concentration of 1M or less. More preferred temperatures and ionic strengths are as detailed above for the releasing step.
The use of such mild conditions for the release of nucleic acid is especially useful for extracting small quantities of nucleic acid, as the extracted DNA or RNA
can be added directly to a reaction or storage tube without further purification steps (e. g. steps necessitated by the use of high ion concentrations in prior art methods), and without the need to dilute high ionic strength (as is the case with prior art methods using high ionic strength to elute the nucleic acid).
Therefore loss of nucleic acid through changing the container, imperfect recovery during purification steps, degradation, or denaturation, and dilution of small amounts of nucleic acid can be avoided. This is particularly advantageous when a nucleic acid of interest is present in a sample (or is expected to be present) at a low copy number, such as in certain detection and/or amplification methods.
Broadly speaking, preferred chemical species for use as charge switch materials in accordance with the invention comprise a positively ionisable nitrogen atom, and at least one, but preferably more than one, electronegative group (such as a hydroxy, carboxy, carbonyl, phosphate or sulphonic acid group) or double bond (e. g. C=C double bond), which is sufficiently close to the nitrogen atom to lower its pKa. It has been found that such molecules tend to have suitable pKa values for the extraction of nucleic acid under mild conditions according to the present invention. Preferably at least one (but more preferably more than one) electronegative group is separated from the ionisable nitrogen by no more than two atoms (usually carbon atoms). Hydroxyl groups are particularly preferred electronegative groups (particularly when several hydroxyl groups are present, e.g. in polyhydroxyl amines, such as Tris (C(CH20H)3-NH2) or Bis-Tris (see below)), as they (1) lower the pKa of the nitrogen atom (e.g. amine group, e.g. from about l0 or 11) to a suitable value around neutral (i.e. pKa of about '7), (2) allow the species to remain soluble/hydrophilic above the pKa, when the nitrogen atom of the amine group loses its positive charge, (3) provide a site for covalent linkage to a solid substrate, e.g. a polycarboxylated polymer (such as polyacrylic acid), and (4) are uncharged at pH values suitable for the releasing step and at which procedures such as PCR are performed (typically pH 8.5); the presence of charged species can interfere with PCR especially. Especially preferred are chemical species having an ionisable nitrogen atom and at least 2, 3, 4, 5 or 6 hydroxyl groups. Further examples of polyhydroxylated amines are dialcohol amine reagents such as diethanol amine. In one embodiment, silane reagents based on these compounds can be used to attach [HO-(CH2)n]z-N-(CH2)m- moieties, where n and m are selected from 1 to 10, to a solid phase. An example of this using 3-bis(2-hydroxyethyl)aminopropyl-triethoxy silane is provided below.
Many standard, weakly basic, buffers are ideal chemical species to provide the ionisable groups of charge switch materials, as they have pKa values close to neutral (i.e.
7) .
for use as a charge switch material, chemical species comprising ionisable groups can be immobilised onto solid supports (e. g. beads, particles, tubes, wells, probes, dipsticks, pipette tips, slides, fibers, membranes, papers, celluloses, agaroses, glass or plastics) in a monomeric or polymeric form via adsorption, ionic or covalent interactions, or by covalent attachment to a polymer backbone which is in turn immobilised onto the solid support. Alternatively, they can be incorporated into solid, insoluble forms (with or without attachment to a polymer backbone) which inherently exhibit charge switching, e.g. beads, particles, tubes, wells, probes, dipsticks, pipette tips, slides, fibers, membranes or plastics.
Solid phase materials, especially beads and particles, may be magnetisable, magnetic or paramagnetic. This can aid removal of the solid phase from a solution containing the released nucleic acid, prior to further processing or storage of the nucleic acid:
Preferably the weakly basic buffers are biological buffers, i.e. buffers from the class of buffers oommonly used in biological buffer solutions. Examples of biological buffers may be found in commercial chemical catalogues, such as the Sigma catalogue.
Leaching (i.e. transfer from the solid phase into solution in the liquid phase) of chemical species used to provide ionisable groups in ion exchange resins is a virtually inevitable phenomenon to some extent, especially when the species are attached to the solid phase by adsorption. Such leaching typically causes impurity in the resultant product, which can lead to significant problems, particularly if the resultant product is intended to be used in PCR (and especially when the species are charged). The use of biological buffers to provide the ionisable groups in charge switch materials can avoid this problem, since leaching of such buffers into the liquid phase will generally not significantly affect the nucleic acid, nor any downstream processes such as PCR to which it might be subjected.
Indeed, many biological buffers are routinely used in PCR
buffers, storage buffers and other buffer solutions.
In a particularly preferred embodiment, the releasing step takes place in a buffer solution containing the same biological buffer that is used in, as or on the charge switch material.
Examples of suitable biological buffers for use in charge switch materials in accordance with the invention, and their pKa values, are as follows:
N-2-acetamido-2-aminoethanesulfonic acid ~~ (ACES), pICa 6. 8;
N-2-acetamido-2-iminodiacetic acid $$ (ADA), pICa 6.6;
amino methyl propanediol t (AMP), pKa 8.8;
3-1,1-dimethyl-2-hydroxyethylamino-2-hydroxy propanesulfonic acid t (AMPSO), pKa 9.0;
N,N-bis2-hydroxyethyl-2-aminoethanesulfonic acid tt (BES), pKa 7.1;
N,N-bis-2-hydroxyethylglycine t (BICINE), pKa 8.3;
bis-2-hydroxyethyliminotrishydroxymethylmethane $$
(Bis-Tris), pKa 6.5;
1,3-bistrishydroxymethylmethylaminopropane $$ (BIS-TRIS Propane), pKa 6.8;
4-cyclohexylamino-1-butane sulfonic acid (CABS), pKa 10.7;
3-cyclohexylamino-1-propane sulfonic acid (CAPS), pKa 10.4;
3-cyclohexylamino-2-hydroxy-1-propane sulfonic acid (CAPSO), pKa 9.6;
2-N-cyclohexylaminoethanesulfonic acid (CHES) pKa 9.6;
3-N,N-bis-2-hydroxyethylamino-2-hydroxypropanesulfonic acid tt (DIPSO), pICa 7.6;
N-2-hydroxyethylpiperazine-N-3-propanesulfonic acid tt (EPPS or HEPPS), pKa 8.0;
N-2-hydroxyethylpipera~ine-N-4-butanesulfonic acid t (HEPBS), pKa 8.3;
N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid tt (HEPES), pKa 7.5;
N-2-hydroxyethylpiperazine-N-2-propanesulfonic acid tt (HEPPSO), pKa 7.8;
2-N-morpholinoethanesulfonic acid $ (MES), pKa 6.1;
4-N-morpholinobutanesulfonic acid tt (MOBS), pKa 7 . 6;
3-N-morpholinopropanesulfonic acid tt (MOPS), pKa 7.2;
3-N-morpholino-2-hydroxypropanesulfonic acid $$
(MOPSO), pKa 6.9;
piperazine-N-N-bis-2-ethanesulfonic acid $t (PIPES), pKa 6. 8;
piperazine-N-N-bis-2-hydroxypropanesulfonic acid tt (P0PS0), pKa 7.8;
N-trishydroxymethyl-methyl-4-aminobutanesulfonic acid t (TABS), pKA 8.9;
N-trishydroxymethyl-methyl-3-aminopropanesulfonic acid tt (TAPS), pKa 8.4;
3-N-trishydroxymethyl-methylamino-2-hydroxypropanesulfonic acid tt (TAPSO), pECa 7.4;
N-trishydroxymethyl-methyl-2-aminoethanesulfonic acid tt (TES), pKa 7.4;
N-trishydroxymethylmethylglycine t (TRICINE), pKa 8.1; and trishydroxymethylaminomethane t (TRIS), pKa 8.1;
histidine*, pKa 6.0, and polyhistidine tt;
imidazole*, pKa 6.9, and derivatives* thereof (i.e.
imidazoles), especially derivatives containing hydroxyl groups**;
triethanolamine dimers**, oligomers** and polymers**; and di/tri/oligo amino acids**, for example Gly-Gly, pKa 8.2; and Ser-Ser, Gly-Gly-Gly, and Ser-Gly, the latter three having pKa values in the range 7-9.
In a preferred embodiment, the buffers marked above with an asterisk (*) are not considered to be biological buffers for the purposes of the invention (whether or not they are designated as such in any chemical catalogue).
In a more preferred embodiment, those marked with two asterisks (**) are also not considered to be biological buffers. Preferred biological buffers are marked with a dagger (t), more preferred buffers are marked with two daggers (tt), still more preferred buffers are marked with a double dagger ($) and most preferred buffers are marked with two double daggers ($~).
These and other chemical species comprising ionisable groups may be coated as monomers onto a solid phase support using covalent, ionic or adsorption interactions.
Additionally or alternatively, they may be coated onto such solid phase supports in polymeric form (preferably following condensation polymerisation), for example by adsorption onto a negatively charged surface (e.g. a surface having exposed COOH or S03 groups), or by covalent attachment. Additionally or alternatively, the chemical species containing ionisable groups may be attached to a polymer (see below) which is then attached to a solid support, e.g. by adsorption or covalent attachment.
Preferably the chemical species or polymer backbones are covalently coupled to the solid support via a hydroxyl group or other group so that the ionisable group having the desired pKa value (usually, but not limited to, a nitrogen atom) remains capable of binding and releasing nucleic acid.
Biological buffers and other chemical species comprising positively ionisable groups may be used in conjunction with a chemical species containing a negatively ionisable group which has a suitable pKa, preferably in the ranges described above. For example a biological buffer (having one or more positively ionisable nitrogen atoms) may be attached to a polymer or other solid phase material which has exposed carboxy groups even after attachment of the biological buffer. Such a material may bind nucleic acids at a low pH when few of the carboxy groups are negatively charged (i.e. few are in the COO- form, most being in the COOH form) and most of the ionisable nitrogen atoms are positively charged. At higher pH the negative charge is stronger (i.e. a greater proportion of carboxy groups are in the COO- form) and/or the positive charge is weaker, and the nucleic acid is repelled from the solid phase.
Chemical species containing ionisable groups (such as the biological buffers listed above) can be attached to a polymer backbone using known chemistries. For example a chemical species containing a hydroxyl group can be attached using carbodiimide chemistry to a carboxylated polymer backbones. Other chemistries include can be employed by someone skilled in the art using other polymer backbones (e. g. based on polyethylene glycol (PEG) or carbohydrate) using a range of standard coupling chemistries (see e.g. Immobilised Affinity Zigand Techniques, Greg T. Hermanson, A. Krishna Mallia and Paul K. Smith, Academic Press, Inc., San Diego, CA, 1992, ISBN 0123423309, which is incorporated herein by reference in its entirety.) Alternatively, the chemical species containing ionisable groups can be polymerised without a backbone polymer, using~cross-linking agents, for example reagents that couple via a hydroxy group (e. g. carbonyldiimidazole, butanediol diglycidyl ether, dialdehydes, diisothiocyanates). Polymers may also be formed by simple condensation chemistries to generate polymeric amino acids with the appropriate pKa e.g. Gly-Gly.
Preferably such immobilisation, attachment and/or polymerisation of the chemical species containing the ionisable group does not affect the pKa of the ionisable group, or leaves it in the desired ranges given above.
For example it is generally preferred not to couple or polymerise the chemical species via a positively ionisable nitrogen atom (in constrast for example to W097/2982). In the practice of the invention, it is especially preferred to immobilise, attach and/or polymerise the chemical species via an hydroxyl group.
A preferred polymeric material is a dimer or oligomer of Bis-Tris, or a material formed by attaching a plurality of Bis-Tris molecules to a polyacrylic acid backbone, e.g. by reacting Bis-Tris monomer with polyacrylic acid using 1-ethyl-3-dimethylaminopropyl carbodiimide (EDC).
The polymer can then be easily separated from the reactants using dialysis against a suitable reagent or water. Preferably the polyacrylic acid has molecular weight of between about 500 and 5 million or more. More preferably it has a molecular weight of between 100,000 and 500,000.
The nature of the resultant Bis-Tris/polyacrylic acid molecule will depend on the ratio of the coupled components, since the polymer will have different properties depending on the proportion of the acrylic acid groups that are modified with Bis-Tris, for example it is desirable for some carboxy groups to remain unmodified, as the presence of these will not prevent the Bis-Tris from binding nucleic acid at low pH (especially if the Bis-Tris is in excess), but their negative charge at higher pHs will assist with release of the nucleic acid. For use in the present invention, the molar ratio of Bis-Tris:carboxy groups (before attachment) is preferably between 5:1 and 1:5, more preferably between 3:1 and 1:3, still more preferably between 2:1 and 1:2, further preferably between 1.5:1 and 1:1.5, and most preferably about 1:1.
The presence of high residual charge (i.e. charged species present in solution along with the extracted nucleic acid) may adversely affect the analysis of nucleic acids by PCR, or interfere with the binding of primers, dNTPs or polymerase to the nucleic acid, or to the sequestration of Mg2+ ions, which are essential to PCR. It is particularly preferable to avoid residual positive charge.
Preferred materials for use in the invention, such as the biological buffers described above, possess minimal residual positive charge (preferably minimal residual charge) at the pH at which the nucleic acid is released, and/or at pHs 8-8.5, making interference with or inhibition of downstream processes unlikely.
Patent application PCT/GB00/02211, of the same inventor, discloses certain methods within the scope of the present invention and is incorporated herein by reference in its entirety as exemplification of the present invention (in all its aspects - see below for other aspects of the invention). In particular, it discloses a method for the extraction of biomolecules from biological material which method comprises contacting the biological material with a solid phase which incorporates histidine or a polyhistidine which will tend to bind nucleic acids at low pH and then extracting the biomolecules bound to the solid phase by elution using an elution solvent which will then release the bound nucleic acids when the pH is increased.
An alternative embodiment of the present invention uses a material which is positively charged across a wide pH
range, such as 0-12 or 0-14 (e. g. an electropositive substance such as a metal oxide, metal, strong or weak base, which lacks a pICa value, or for which the pFCa value is at an extreme of high pH. Such a positively charged material is combined with negatively ionisable material having a pKa intermediate between the pH values at which it is desired to bind and release nucleic acid, or slightly below the pH at which it is desired to bind nucleic acid. This combination of materials allows nucleic acid to be bound at certain pH values, around and below the pKa of the negatively ionisable material, when there are fewer negatively charged groups, but allows the nucleic acid to be released when the pH is increased and a greater number of the ionisable groups are negatively charged. For example, the combination of iron II, III
oxide and polycarboxylates (see Examples) binds nucleic acid at pH 4, when a relative scarcity of negative charges allowing the positively charged iron oxides to bind the nucleic acid. When the pH is increased to around 8, a large proportion of the carboxy groups become negatively charged and, despite the remaining presence of positive charges on the iron oxides, the reduction in overall positive charge allows the nucleic acid to be released.
Further examples of charge switching molecules for nucleic acid purification are based on detergents or surfactants that have a hydrophobic portion and a hydrophilic portion which comprises a positively ionisable group with a suitable pKa, e.g. decyl methyl imidazole or dodecyl-Bis-Tris. These detergents/surfactants can be adsorbed onto surfaces e.g.
plastic via their hydrophobic portions and the hydrophilic (ionisable) portions can be used to capture nucleic acid.
Another family of suitable materials for capture and easy release of nucleic acids are carbohydrates e.g.
glucosamine, polyglucosamine (including chitosans), kanamycins and their derivatives, i.e. sugar ring based structures containing one or more nitrogen atoms surrounded by hydroxyl groups which may also contain other groups such as acetate or sulphate groups to provide a suitable pKa for binding and release of nucleic acids.
Another group of materials with suitable pKa values are nucleic acid bases, e.g. cytidine (pKa 4.2). These can be immobilised via hydroxy groups to a polymer or solid phase carboxy group using carbodiimides.
A still further group of materials having members with suitable pKa values are heterocyclic nitrogen-containing compounds. Such compounds may be aromatic or aliphatic and may be monomers, oligomers or polymers, such as morpholine-, pyrrole-, pyrrolidine-, pyridine-, pyridinol-, pyridone-, pyrroline-, pyrazole-, pyridazine-, pyrazine-, piperidone-, piperidine-, or piperazine-containing compounds, e.g. polyvinylpyridine.
Such compounds may be substituted with electronegative groups to bring the pKa values) of the ionisable nitrogen atoms) into an acceptable range, e.g. as defined above. However, in some compounds this may not be necessary, the pKa already being in such a range.
A still further group of solid phases for binding nucleic acid have surface amine groups, and in particular amine groups which are not polyamines. These monoamine groups can be represented by the formula -NR1R2, where R1 and RZ
are hydrogen or substituted or unsubstituted alkyl.
Although these materials typically have pKa values which at higher than those of materials used in preferred embodiments of the invention, they can be employed in the extracting of nucleic acid, optionally employing them with negatively charged species as described herein to modify the overall pKa of the solid phase.
A further group are materials that provide ionisable groups capable of acting as charge switch materials and binding nucleic acid are dyes, and in particular biological dyes having pKas between 5 and 8. These materials can be immobilised or coated on solid phases.
An example of a biological dye is Neutral Red used in Example 46 below.
Preferred materials for use in accordance with the invention are hydrophilic, for example comprising charge switch materials which are (or which comprise chemical species which before immobilisation or polymerisation are) water soluble.
Once a suitable solid phase has been prepared, comprising a charge switch material, repeated capture and release of nucleic acids can be performed by adjusting the pH up or down. Thus sequential reactions or analysis can be performed on the nucleic acids using the same solid phase. For example, DNA can be isolated from a biological sample using a PCR tube comprising a charge switch material. Then, following PCR, the amplified DNA
product may be isolated from the buffer constituents or primers by adjusting the pH in the same tube.
Particularly preferred solid phase materials are non-porous. Porous supports are commonly used for isolating proteins, which can be trapped in the pores of the support. However, nucleic acids tend to be too big to enter into pores of commonly used such supports, and will therefore become bound to the surface of the support, potentially trapping impurities in the pores.
The method can be used to separate single stranded RNA or DNA from double stranded DNA, because of the different charge densities on single and double stranded molecules, by appropriate manipulation of the pH or salt concentration. Typically, single stranded molecules will be released from binding to the solid phase at a lower pH
than double stranded molecules.
In some circumstances, for example for the construction of gene chips, and for the preparation of probes, it may be desirable to produce single stranded DNA.
Manipulation of pH andlor ionic strength can assist in purification and release of single stranded nucleic acid.
The method of the invention may comprise a prior step of converting double stranded nucleic acid in the sample to single stranded nucleic acid (preferably using a strong base, e.g. 100mM NaOH, or a weak base at high temperature, e.g. 60-100°C). The solid phase material is preferably then added simultaneously with a buffer which changes the pH of the sample to the pH for binding single stranded nucleic acid (typically a pH of 4-7).
The materials described herein may also be employed to capture nucleic acids in the liquid phase where binding leads to a cross-linked lattice large enough to separated from the liquid phase, e.g. by filtration or centrifugation.
Accordingly, in a second aspect, the present invention provides a method for extracting nucleic acid from a sample containing nucleic acids, which method comprises:
contacting the sample with a charge switch material at a first pH at which the charge switch material has a positive charge and will bind negatively charged nucleic acid; and then releasing the nucleic acid at a second, higher pH at which the charge is neutral, negative or less positive than at the first pH, wherein the charge switch material is soluble at said first pH, and wherein the combination of the charge switch material and the bound nucleic acid is insoluble at or above said first pH
and below said second pH.
Preferred features of the method are as set out above, with the exception of the charge switch material being formed into, immobilised on, or attached to, a solid phase material.
Usually the charge switch materials will be soluble at the second pH, and will remain in solution with the nucleic acid upon release of the nucleic acid; the use of a weakly basic buffer (optionally bound to a soluble backbone, e.g. polyacrylic acid) as the charge switch material can avoid problems of contamination as described above.
The methods of the invention preferably include one or more washing steps between the binding and releasing steps. Such (a) washing steps) will generally be carried out at said first pH, or a pH above said first pH
but lower then said second pH, such that the nucleic acid is substantially not released during the washing step(s).
As has been indicated previously, the methods of the invention are particularly suitable for extracting nucleic acid which is then stored or further processed (e. g. by PCR), particularly when the charge switch material is in the form of e.g. a tube or well in which such storage and/or processing can occur. For the avoidance of doubt, however, it is emphasised that the releasing step and any subsequent storage or processing need not be carried out as discrete steps, but can coincide, when said storage or processing occurs at a pH
at which release of the nucleic acid occurs. For example, the method of the invention includes binding nucleic acid to a charge switch material coated on or otherwise provided lay a PCR tube, washing the bound nucleic acid, and then without a separate releasing step commencing the PCR reaction using a PCR buffer which causes release of the nucleic acid.
In a further aspect, the present invention provides novel charge switch materials for use in the methods of the receding aspects. It further comprises the use of such charge switch materials in such methods. All preferred features of the charge switch materials described in above in the context of the methods apply equally and independently to the present aspect of the invention (i.e. preferred combinations of features may be different in relation to this aspect from the preferred combinations in relation to the method aspects).
In a further aspect, the present invention provides a container (preferably a PCR or storage tube or well, or a pipette tip) coated with, comprising or formed from a charge switch material, preferably a charge switch material comprising a biological buffer.
The following description is directed particularly to the extraction of nucleic acid from blood, but applies also to the extraction of nucleic acid from any liquid sample, particularly biological samples or samples produced during laboratory techniques, such as PCR.
The method is particularly useful if the biological material is blood, but the method can be used for a range of applications substances such as plasmid and vector isolation and plant DIVA extraction.
Preferably the cells in the blood are lysed to release nucleic acids and known lysing agents and methods can be used, such as contacting with ionic and non ionic detergents, hypotonic solutions of salts, proteases, chaotropic agents, solvents, using pH changes or heat. A
method of lysing cells to isolate nucleic acid is described in WO 96/00228.
When the biological material consists of blood the samples can optionally be diluted with water or other diluent in order to make it easier to manipulate and to process.
Dilutions up to ten times can be used and in general more dilution can be better and it is a feature of the present invention that it allows low dilution of blood to be possible.
The solid phase with which the blood is contacted, can be a formed of a material which has a natural affinity for nucleic acids or it can be formed of a material which has its surface treated with an agent which will cause nucleic acids to bind to it or increase its affinity for nucleic acids. Suitable materials include controlled pore glass, polysaccharide (agarose or cellulose), other types of silica/glass, ceramic materials, porous plastic materials such as porous plastic plugs which in a single moulded part or as an insert in a standard tube, polystyrene beads para magnetic beads etc. The size and porosity is not critical and can vary and be selected for particular applications.
Suitable means for treating the surface of the solid phase or for derivatising it include treating it with a substance which can introduce a charge e.g. a positive charge on the surface or a hydrophilic or hydrophobic surface on the solid phase e.g. hydroxyl groups, nitrate groups, autoreactive groups, dyes and other aromatic compounds.
In a preferred embodiment of the invention the solid phase will cause DNA to be bound to it at one pH in preference to contaminants in the blood sample and will allow the bound nucleic acid to be released when it is contacted with an eluant at a different pH. This system can be used with a solid phase which incorporates histidine or a polyhistidine which will tend to bind nucleic acids at low pH e.g. less than 6 and will then release the bound nucleic acids when the pH is increased e.g. to greater than 8. Alternatively the nucleic acids are bound at substantially neutral pH to an aminated surface and released at very high pH.
In another embodiment of the invention a plastic moulding can incorporate a binding agent e.g. in a well in a plate etc. so that the binding agent is incorporated in the surface, the blood sample is then contacted with the surface so as to cause nucleic acids to be bound to the surface. The blood sample is then removed and the surface treated with an eluting agent to release the bound nucleic acids. When the surface is part of a well in a multi- well plate, the total system can be readily adapted for rapid large scale sampling and extraction techniques, Binding agents which can be used include charge switchable ion exchange resins using a positively charged solid phase that can be reversed or made neutral by changing the pH above its pKa. e.g. nucleotides, polyamines, imidazole groups and other similar reagents with a suitable pKa value.
Also, nucleic acids can be bound by intercalation using a variety of intercalating compounds incorporated into the solid phase e.g. actinomycin D, ethidium bromide etc.
In a further embodiment of the invention a plastic surface can be modified to include functional groups. The plastic can be any plastic used for containing samples e.g. polypropylene. The functional groups can be positively or negatively charged so as to bind the nucleic acids in the correct buffer solution.
Alternatively the functional groups can be chemical groups capable of covalent coupling to other ligands or polymers.
When the plastic is used in a plastic moulding e.g. in a well in a plate, or as a polymerase chain reaction (PCR) tube, the surface characteristics of the plastic can be suitably modified for use in the present invention by including or adding the appropriate chemicals in the moulding compound e.g. as in an injection moulding compound.
When this is used in a PCR tube or in a deep well plate the tubes or wells can be used to isolate and immobilise small quantities of DNA or RNA generating a pure template for subsequent PCR or other genetic analysis and manipulation.
When the plastic is polypropylene e.g. it is in the form of a thin walled PCR tube the polypropylene surface can be modified by oxidising the surface with an oxidising agent such as potassium permanganate and sulphuric acid to create a carboxylated surface (C00H groups). This tube can then be used to improve the isolation of DNA from solutions or from crude samples e.g. blood. By adjusting the pH, di-electric constant, solubility or ionic strength the DNA or RNA can be immobilised on the walls of the tube, washed free of contaminants, ready for PCR
or other analytical techniques.
The carboxy groups can be further modified by covalently coupling an anionic group such as imidazole or polyhistidine or any strong or weak ion exchanger, to allow binding of nucleic acids by a charge interaction.
This tube could then be used to improve the isolation of DIVA from solutions or from crude samples e.g. of blood.
Again by adjusting the pH, di-electric constant, or ionic strength the DNA or RIVA can be immobilised on the walls of the tube, washed free of contaminants, ready for PCR
or other analytical techniques.
The nucleic acids can be eluted with in a low salt buffer so that it is ready for PCR or other analysis.
The solid phase can be contacted with a blood sample by mixing with the solid phase in a mixingl stirring device, by passing the blood sample over the solid phase or the solid phase can be paramagnetic and manipulated by a magnetic field. Although the invention is particularly suitable for the separation or isolation of nucleic acids from blood it can be used with a range of biomolecules particularly those that require removal of cell wall debris or insoluble particles.
In a preferred embodiment of the invention the solid phase is in granular form in a column and the blood sample is drawn up through the column by means of a pressure differential being applied through the column, the blood sample is drawn up with air and the granular solid material can become fluidised thus increasing the mixing and contacting rates and minimising clogging.
The method of the invention is suitable for use in a multi-well format when a series of extractions from different samples can take place substantially simultaneously and this will facilitate the automation of the extraction process allowing rapid high throughput extraction to take place and to allow combinational chemistry to be performed. This will enable there to be a high throughput in a standard well array e.g. an eight by twelve array so that a large number of sample types can be treated automatically at the same time.
Detailed Description The invention, in its various aspects, will now be described in detail, by way of example only.
Example 1: Extraction of nucleic acids from whole blood A charge switchable ion-exchanger was prepared by covalently coupling polyhistidine to 100 um glass beads using glutaldehyde by mixing 1 gram of the aminated glass beads with 0.01 o(v/v) glutaldehyde in 0.1M sodium bicarbonate at pH8 containing 20mg polyhistidine. After overnight incubation the beads were washed exhaustively to remove non-covalently bound material and stored in lOmM MES, pH5 containing 0.1 0 (v/v) Tween 20.
About 300mg of the 100pm derivatised glass beads were added to a lml plastic column enclosed at both ends.
A blood sample was incubated with an equal volume of lOmM
MES pH5, containing 1o Tween 20, proteases (200ug/ml) and 1 mM EDTA. After digestion is complete the blood was sucked up the column containing the glass beads and the DNA became immobilised allowing the contaminating proteins to pass through to waste.
The glass beads containing the immobilised DNA were washed with a buffer comprising lOmM MES pH5, containing 1o Tween 20, and 1 mM EDTA and this was repeated until the wash solution was colourless.
After washing, the beads were dried with air and DNA
eluted with a small quantity of lOmM Tris HCl, pH 8.5 and collected in a sterile tube ready for analysis. Thus the DNA were separated from the blood. For different biomolecules, the buffer etc. can be suitably modified.
Example 2:Polyhistidine on carboxylated beads One gram of carboxylated paramagnetic beads were washed in 50mM imidazole buffer pH6 and then mixed with 100mg of polyhistidine in 50m1 of 50mM Imidazole buffer pH 6. A
chemical coupling agent was added (EDC) at a final concentration of 5mg per ml and mixed overnight. The beads were washed in water, 0.5M sodium chloride, 10 Tween 20, 100mM Tris HCl pH 8 and stored in lOmM MES, 0.1o Tween 20 pH5.
To extract DNA from blood, lmg of beads were mixed with blood diluted in 10o Tween 20 with 25mM MES, 1mM EDTA pH
5. The beads were separated with a magnet and washed by resuspending in 1mM MES, 0.1o Tween 20. To elute the DNA
the beads were resuspended in lOmM Tris HCl pH 8.5 and separated with magnet leaving the DNA in solution.
Example 3: Bis-Tris solid phase magnetic beads 200mg of carboxylated 1 ~,m magnetic particles were reacted in a one step procedure with 100mg of Bis-Tris and 100mg of the carbodiimide, EDC, in 50mM imidazole buffer pH6Ø Following an overnight incubation, the magnetic particles were washed and used to isolate Plasmid DNA.
An alkaline lysis method was used to prepare a cleared 5m1 bacterial lysate generating a supernatant containing the plasmid in 0.5M potassium acetate, pH5. To the supernatant, 2.5mg of magnetic particles were added and mixed for 1 minute. After magnetic separation and washing with water pHS, the pure plasmid DNA was eluted off in 2001 of lOmM Tris.HCl pH 8.5.
The magnetic beads were also used to extract DNA directly from whole blood using a detergent based digestion reagent containing proteinase K.
Example 4: Tricine on solid phase magnetic beads 50mg of carboxylated 1 ~,m magnetic particles were reacted in a one step procedure with 50mg of Tricine and 100mg of the carbodiimide, EDC, in 50mM imidazole buffer pH6Ø
Following an overnight incubation, the magnetic particles were washed and used to isolate plasmid DNA. An alkaline lysis method was used to prepare a cleared 5m1 bacterial lysate generating a supernatant containing the plasmid in 0.5M potassium acetate, pH5. To the supernatant, 2.5mg of magnetic particles were added and mixed for 1 minute.
After magnetic separation and washing with water pH5, the pure nucleic acids were eluted off in 200.1 of lOmM
Tris.HCl pH 8.5.
Example 5: Bis-Tris solid phase polystyrene beads 1 gram of carboxylated 60~,m polystyrene particles were reacted in a one step procedure with 500mg of Bis-Tris and 500mg of the carbodiimide, EDC, in 50mM imidazole buffer pH6Ø Following an overnight incubation, the particles were washed and used to isolate plasmid nucleic acids as described above.
Example 6: Bis-Tris polymer Bis-Tris monomer was converted into a polymer by mixing together 160mg of polyacrylic acid with a molecular weight of 240,000, 1.6g of Bis-Tris and 1.6g of EDC in 50mM imidazole pH6Ø Following an overnight incubation, the mixture was dialysed in water. The purified polymer was then coated onto magnetic COOH beads or used in the liquid phase to bind genomic DNA from blood. A 5m1 blood sample was centrifuged to obtain the nuclei and WBC
population and the resulting pellet digested with 1o SDS.
Following precipitation with potassium acetate the cleared supernatant was mixed with either 25mg of magnetic-Bis-Tris or about 250~g of poly-Bis-Tris as a liquid. In both cases the captured DNA could be separated, washed in water and then redissolved in lOmM
Tris HC1 pH8.5 in a pure form.
Example 7: Insoluble Tris HC1 polymer In this example an insoluble polymer was made with inherent charge switching properties by mixing 80mg of polyacrylic acid with 800mg of Tris HC1 and 800mg of EDC
in 50mM Imidazole pH6. The insoluble precipitate that formed generated a particulate solid phase that was used to capture DNA and release it in a similar manner to that described in Example 4 for genomic DNA.
Example 8: Immobilised poly Bis-Tris on pipette ti~
A solution of poly Bis-Tris at 1mg/ml, prepared as in Example 2, in O.1M sodium bicarbonate pH8 incubated at 60°C for 8 hours with twenty 200~Z1 polyproplylene pipette tips. The tips were then rinsed and used to capture about 150ng of plasmid DNA from a cleared bacterial lysate by pumping up and down ten times. After a quick wash with water pHS, the DNA was eluted in 50,1 of lOmM
Tris pH 8.5.
Example 9: Immobilised poly Bis-Tris on PCR tubes A solution of poly Bis-Tris at 1mg/ml, prepared as in Example 2, in 0.1M sodium bicarbonate pH8 incubated at 60°C for 8 hours in a 200u1 PCR plate of 8x12 tubes.
After rinsing, the tubes were used to bind genomic DNA
from a sample prepared according to Example 4. About 50ng of DNA was subsequently eluted off per tube using lOmM Tris HCl pH8.5.
Example 10: Charge switch detergents in liquid phase A blood sample was prepared as described in Example 4 and to the resulting supernatant decyl imidazole was added at pH 4 causing precipitation of the DNA. The DNA pellet was collected by centrifugation and redissolved in lOmM
Tris pH 8.5.
Example 11: Charae switch detergents on solid t~hase Decyl imidazole was adsorbed onto a 200u1 plastic pipette tip by soaking in a 1o solution at pH4 in 0.1M sodium acetate. A blood sample was prepared as described in Example 3 and the tips were used to bind the DNA by repeated pumping and sucking. After a wash with water, about 50ng of DNA was recovered in water at pHlO.
Example 12: Polyglucosamines l0mg of low molecular weight Chitosan was dissolved in acidified water and then 50mM imidazole pH5.5, this was mixed with 100mg of carboxy 1 ~,m magnetic beads and with 20mg of the carbodiimide EDC in 50mM imidazole pH5.5.
Following an overnight incubation, the beads were washed and resuspended in lOmM MES pH5. To bind genomic DNA, 2mg of magnetic particles were added to a supernatant prepared by methods described earlier in Example 1, after magnetic separation, the DNA was eluted using 100mM
Tris.HCl pH 9.5.
Example 13: Kanamycin A solution of genomic DNA was prepared as described in example 3. To this sample 2mg of Kanmycin was added at a concentration of l0mg/ml. The resulting precipitate of DNA was filtered, washed in water at pH5 and re-dissolved in water at pHlO.
Example 14: Magnetisable iron oxides in carboxylated polystrene A 5m1 plasmid mini-prep was prepared using standard alkaline lysis reagents to generate a cleared lysate with a potassium acetate composition of 0.5M pH4. To this cleared supernatant, 2.5mg of commercially available l~,m carboxylated polystyrene magnetisable particles were added to bind the plasmid DNA. The particles were washed with water at pH4 and then the DNA eluted using lOmM Tris HC1 at pH 8.5. Typical UV ratios at 260 and 280nm were 1.7-2.0, indicating pure nucleic acids with a single band observed with standard gel electrophoresis.
Example 15: Titanium dioxide in polystyrene microtitre l~ ates A solution of DNA at 100~,g per ml in 0.1M Potassium Acetate pH4 was allowed to stand for 1 hour in a 300u1 flat bottomed microtitre plastic plate, the plastic plate contained titanium oxide which was incorporated as a powder in the plastic when the plate was formed. After washing at pH4, the DNA was recovered with water at pHlO
and 2m1 measured at 260nm versus a plain polystyrene plate with no titanium oxide. Approximately, 50ng of DNA
was recovered per 300u1 well for the plate incorporating the titanium oxide compared to zero for the plain polystyrene plate.
Example 16: Cytidine coupled to magnetic beads 1 gram of carboxylated 1 ~m magnetic particles were reacted in a one step procedure with 500mg of Cytidine and 500mg of the carbodiimide, EDC, in 50mM imidazole buffer pH6Ø After thorough washing, the beads were used to bind nucleic acids from a plasmid preparation as described in example 1 and recovering the pure nucleic acids in water at pHlO.
Example 17: Polyvinyl pyridine (PVP) 20mg of commercially available PVP beads was mixed with the supernatant containing genomic DNA from a 5m1 blood extraction described in Example 4. After allowing the DNA to bind, the beads were washed with water at pH5 and the DNA recovered using water at pHlO. Ultra violet analyisis at 260 and 280nm indicated a purity ratio of 1.65.
Example 18: Separation of RNA and DNA
A solution of tRNA and sheared genomic DNA was prepared at 30~,g per ml in 50mM Potassium acetate buffer pH6.5 with 1M sodium chloride. Approximately 4mg of magnetic polyhistidine beads were mixed with 1m1 of the nucleic acid solution for one minute until binding was complete.
The beads were then thoroughly washed with water at pH5.
To elute the bound material, the beads were mixed with 300,1 of lOmM Tris.HCl, lOmM NaCl, pH8.5. Gel analysis showed that most of the tRNA remained in solution and was not bound to the beads. The eluted material contained mostly genomic DNA with little or no tRNA.
Example 19: PCR tubes with surface amine groups PCR tubes were modified to provide surface amine groups for binding DNA or RNA. About 2001 of supernatant from a cleared lysate from a pUCl9 plasmid prep. was allowed to incubate in the tube for 10 minutes. The tube was then washed with water and the bound DNA on the walls of the tube was treated with a PCR reaction mixture with primers for pUCl9. After the PCR reaction mixture was completed, the tubes contents were run on a 1o gel with ethidium bromide. The gel showed a strong band indicating that the PCR reaction had been successful.
Example 20: Magnetic beads With surface amine groups Commercially available 4um magnetic beads with surface amine groups for binding DNA or RNA were mixed with about 2001 of supernatant from a cleared lysate from a PU19 plasmid prep. and allowed to incubate in the tube for 10 minutes. Then 5u1 beads (about 100~g of beads) were washed with water and the beads added to a PCR reaction mixture with primers for pUCl9. After the PCR reaction mixture was completed, the mixture was run on a 1o gel with ethidium bromide. The gel showed a strong band indicating that the PCR reaction had been successful.
Example 21: Magnetic beads with surface amine groups Commercially available 4um magnetic beads with surface amine groups for binding DNA or RNA were mixed with about 200p1 of supernatant from a cleared lysate from a pUCl9 plasmid prep. and allowed to incubate in the tube for 10 minutes. Then 5u1 beads (about 100~g of beads) were washed with water and the beads mixed in 100u1 of water at pH 10 using NaOH to elute enough DNA for a PCR
reaction . About 10p1 of the eluted DNA was mixed with a PCR master mix with primers for PU19. After the PCR
reaction mixture was completed, the mixture was run on a 1% gel with ethidium bromide. The gel showed a strong band indicating that the PCR reaction had been successful.
l0 Example 22: Magnetic beads with surface amine groups Commercially available 4~Zm magnetic beads with surface amine groups for binding DNA ox RNA were mixed with about 200u1 of supernatant from a cleared lysate from a pUCl9 plasmid prep. and allowed to incubate in the tube for l0 minutes. Then 5~1 beads (about 100ug of beads) were washed with water and the beads mixed in 100u1 of a 10 times strength PCR buffer to elute enough DNA for a PCR
reaction. About 5~Z1 of the eluted DNA was mixed with a 45u1 of water with primers for pUCl9. After the PCR
reaction mixture was completed, the mixture was run on a 1o gel with ethidium bromide. The gel showed a strong band indicating that the PCR reaction had been successful.
Example 23: Polyacrylic acid-diamine ethylene mixed charge polymer A mixed charge polymer for binding DNA or RNA was prepared by mixing 100mg of polyacrylic acid Mr 240K with 20mg of diamine-ethylene in 50m1 of 0.1M imidazole buffer pH 6.5 and adding 300mg of EDC cross linking agent.
After overnight incubation the mixture was dialyzed exhaustively and used to bind DNA by adding 100~z1 to a white blood cell digest containing 7M Urea at pH 5. The resulting complex of DNA and polymer was collected by centrifugation, washed in water at pH 5 and re-dissolved in water at pH 10.
Example 24: Polyacrylic acid-triethanolamine mixed charge polymer A polymer for binding DNA or RNA was prepared by mixing 100mg of polyacrylic acid Mr 240K with 1g of Triethanolamine in 50m1 of 0.1M imidazole buffer pH6.5 and adding 1g of EDC cross linking agent. After overnight incubation the mixture was dialyzed exhaustively and used to bind DNA by adding 1001 to a white blood cell digest containing 7M Urea at pH 5. The resulting complex of DNA and polymer was collected by centrifugation, washed in water at pH 5 and re-dissolved in water at pH 10.
Example 25: Polyacrylic acid-Bis-Tris mixed charge polymer A mixed charge polymer for binding DNA or RNA was prepared by mixing 100mg of polyacrylic acid Mr 240K with 20mg of Bis-Tris in 50m1 of O.1M imidazole buffer pH 6.5 and adding 300mg of EDC cross linking agent. After overnight incubation the mixture was dialyzed exhaustively and used to bind DNA by adding 1001 to a white blood cell digest containing 7M Urea at pH 5. The resulting complex of DNA and polymer was collected by centrifugation, washed in water at pH 5 and re-dissolved in lOmM Tris HC1 pH 8.5.
Example 26: Carboxyl groups magnetic beads and Bis-Tris A mixed charge polymer of C00H and Bis-Tris as previously described was coupled to a COOH magnetic beads by adding 100mg of beads with l0mg of polymer and 20mg of EDC in O.1M imidazole at pH6. The beads showed significant binding of purified DNA at pH 5 and release in lOmM Tris HCl pH 8.5.
Example 27: Polyacrylic acid and poly Bis-Tris A soluble polymer of poly Bis-Tris was formed in-situ inside a porous plastic plug by mixing polyacrylic acid with Bis Tris in the ratio of 1:10 in 0.1M imidazole buffer pH6.5. Then EDC was added in a 10 fold excess to polyacrylic acid to form the polymer. This was washed in l6mM potassium acetate pH 4 to remove unbound material and the plug then used to bind DNA from a 7M Urea digest of white blood cells at pH 5. The DNA could be eluted off the plug by increasing the pH to 8.5 using lOmM Tris HCl.
Example 28: Polyacrylic acid and poly Bis-Tris An insoluble polymer of poly Bis-Tris was formed in situ inside a porous plastic plug by mixing polyacrylic acid with Bis Tris in the ratio of 1:10 in O.1M imidazole buffer pH 5.5. Then EDC was added in a 10 fold excess to polyacrylic acid to form the polymer. This was washed in l6mM potassium acetate pH 4 to remove unbound material and the plug then used to bind DNA from a 7M Urea digest of white blood cells at pH 5. The DNA could be eluted off the plug by increasing the pH to 8.5 using IOmM Tris HCl.
Example 29: Magnetic beads derivatised with Bis-Tris A cleared lysate supernatant from an E. coli culture containing pUCl9 plasmids was prepared using standard alkaline lysis reagents. About 501 of the cleared supernatant at pH4 was mixed with 0.25mg of magnetic beads derivatised with Bis-Tris to bind the plasmid DNA.
After washing the impurities away with water, the beads with DNA were then mixed in a PCR reaction in a PCR tube and transferred to a Thermal Cycler for amplification using appropriate primers. Following PCR, the pH was adjusted to 4 using potassium acetate and the PCR product was bound to the same magnetic beads. The beads were washed with water and the clean PCR product was eluted in lOmM Tris HCl pH8.5 for analysis on a gel and by sequencing. The final results showed that the beads could purify the plasmid, then allow PCR in the presence of the beads and finally clean up the PCR product by re-binding and elution of the PCR product ready for detection or further manipulation.
Example 30: Microtitre plate using poly Bis-Tris A carboxylated microtitre plate was prepared by plasma treatment and washed with a solution of poly Bis-Tris at 0.5mg/ml in lOmM potassium acetate pH 5. After washing away unbound material, a solution of blood was added diluted 10 fold in lOmM ammonium carbonate/bicarbonate pH7 with 1o Tween20. After 30 minutes incubation time, the DNA became immobilised on the walls of the wells and the contaminants washed away with water. The DNA was then recovered by mixing with lOmM Tris HC1 pH8.5.
Example 31: Plastic tip using poly Bis-Tris A solution of human DNA at 50ug/ml in 200mM potassium acetate buffer pH5 was mixed with an equal volume of isopropanol. The resulting precipitate was separated using a COOH plastic tip coated with poly Bis-Tris.
After removing all waste liquid, the precipitate of DNA
was re-dissolved in lOmM Tris HCl pH8.5.
Example 32: Sintered disk with poly Bis-Tris Some magnetic beads modified with Bis-Tris were dried in air and then ground using a pestle and mortar. This powder was mixed with some plastic granules and formulated into a sintered disc approximately 3mm thick and 6mm in diameter. The disc could then be used to bind and elute DNA from a solution of calf thymus DNA at 50~g/ml pH4 using lOmM Tris HCl pH8.5 to recover the DNA.
Example 33: Polyhydroxlated amines on silica 1 gram of silica particles of about 60~m in diameter was treated with 10o v/v 3-bis(2-hydroxyethyl)aminopropyl-triethoxy silane in acetone at 60C for l8hours. The modified particles were washed in acetone then water and used for binding calf thymus DNA at 50ug/ml in lOmM
potassium acetate pH4. The DNA was subsequently released at pH 10 in lOmM Tris HC1.
Example 34: Differential binding using magnetic beads with Bis-Tris Differential binding of RNA and DNA from E. coli was demonstrated by preparing cleared bacterial lysates using standard alkaline lysis reagents and ammonium acetate added from 0 to 500mM as a final concentration. About 0.5mg~of Bis-Tris magnetic beads was added to the mixtures to bind the nucleic acids. After washing in water, the beads were eluted in lOmM Tris HC1 pH8.5. The gel results showed that by increasing salt, reduced the amount of RNA bound to the beads relative to DNA, confirmed by measuring the ratio of dsDNA to single stranded DNA/RNA using UV at 260nm and pico green binding.
Example 35: Filter with poly Bis-Tris A 3 micron glass fibre paper filter was soaked in an excess of poly Bis-Tris at 0.5mg/ml in lOmM potassium acetate pH 4 and air dried for 3 days. A 50~g spot of DNA was placed on the filter and dried again so that it may be stored and archived. To recover the DNA, the filter was washed in water and then 2m1 of lOmM Tris HCl pH 8.5 allowed to soak in for 10 minutes and the resulting UV absorbance at 260nm indicated an 800 recovery of DNA.
Example 35: Magnetic beads with Bis-Tris A buccal cell swab was used to collect a sample and placed in digest buffer containing lml of 20mM Ammonium bicarbonate, 1% Tween 20 and proteinase K at 200~g/ml at pH7. After 20 minutes at 50°C, the liquid was transferred to a new tube containing potassium acetate pH
4 and 1mg of magnetic Bis-Tris beads. After a brief incubation the beads were separated with a magnet and washed with water. The DNA or RNA was eluted off using lOmM Tris HCl pH8.5 and visualised on a gel and tested by PCR.
Example 36: Magnetic beads with poly Bis-Tris A 501 blood sample was diluted with a 20 fold excess of 0.010 SDS, lOmM Tris HCl pH 7 and incubated for 5 minutes at room temperature. A magnetic bead coated with poly Bis-Tris was used to capture the DNA and after washing with water it was eluted off using lOmM Tris HCl pH 8.4.
Example 37: Magnetic bead with carboxyl groups and Bis-Tris A magnetic bead was prepared with both COOH and Bis-Tris ligands determined by binding of Congo Red and Neural Red dyes. Purified genomic DNA at 50~g/ml in acetate buffer pH 4 was mixed with lmg of beads and the subsequent complex of bound material was washed and then the DNA
recovered by eluting with 100~z1 of lOmM Tris HCl pH 8.5.
This was repeated with a plasmid extraction from E. Coli containing pUCl9 and pure plasmid was released in the Tris elution buffer as previously described.
Example 38: Silica particles with carboxyl groups and Bis-Tris 1 gram of silica particles of about 60microns in diameter with surface COOH groups were mixed with 1 gram of Bis-Tris in 20m1 of 0.1M imidazole pH6 and 200mg of EDC. The modified particles were washed and used to bind and elute pUCl9 plasmid from a cleared lysate prepared using alkaline lysis reagents.
Example 39: Silica particles with surface amine groups and polyacrylic acid 1 gram of silica particles of about 60 microns in diameter with surface amine groups were mixed with 1 gram of polyacrylic acid Mr 240IC in 20m1 of 0.1M imidazole pH
6 and 200mg of EDC. The modified poly COON particles were washed and were mixed with lgram of Bis-Tris in 20m1 of 0.1M imidazole pH6 and 400mg of EDC. The modified particles were used to bind and elute pUCl9 plasmid from a cleared lysate prepared using alkaline lysis reagents.
Example 40: Differential binding using magnetic Bis-Tris beads Differential binding and elution of RNA and DNA was demonstrated from a plasmid prep. by adjusting the pH for binding near to the pKa of the magnetic Bis-Tris beads.
Alkaline lysis reagents were prepared using acetate Precipitation buffers at pH 4.0, 4.2, 4.4, 4.6, 4.8 so the final pH for binding plasmid was between 4 and 6 after neutralisation. By measuring single stranded RNA
and double stranded DNA after elution from the beads, an estimate of the relative ratios of each was calculated.
In this case, it was found that the RNA binding or elution remained fairly constant while the DNA binding or elution decreased with increasing pH.
Example 41: PCR clean up with magnetic beads with Bis-Tris A PCR clean up reaction was demonstrated by preparing a 600bp and l.SKb PCR reaction for pUCl9 using 401 for purification. To the 40u1 of PCR reaction, 500p1 of about 0.5M acetate buffer pH4 was added and 0.25mg,of Bis-Tris magnetic beads. The beads bound the PCR product and were washed clean with water and eluted in 501 of lOmM Tris HC1 pH 8.5.
Example 42: CMC polymer and Bis-Tris beads A carboxy methyl cellulose polymer, grade 9H4F, was used to prepare a 1o solution in water. The mixture was then added dropwise to a solution of 1M calcium chloride to form a polyelectrolyte complex with surface COOH groups.
The resulting beads were then converted to Bis-Tris beads using an excess of Bis-Tris and EDC in an imidazole buffer pH 6. The beads showed binding and release of pure DNA using pH 4 and pH 8.5 buffers respectively.
Example 43: Insoluble Bis-Tris An insoluble form of poly Bis-Tris was prepared by mixing 160mg of polyacrylic acid Mr 240K, with 1.6 g of Bis Tris in 50m1 0.1M imidazole at pH 5.9 with 1.6 g of EDC. The precipitated polymer was collected by centrifugation and the small particles showed binding and release of DNA at pH 4 and pH 9 respectively.
Example 44: Magnetic beads with Bis-Tris To test whether oligonucleotides could bind to modified magnetic beads, 1mg of Bis-Tris beads were mixed with l0ug of pUCl9 PCR primers in lOmM potassium acetate pH4.
After washing with water pH4, the beads were eluted with lOmM Tris HCl pH8.5 and the UV reading indicated a recovery of 5~g of DNA. A 1o gel containing Sybr green dye also showed a strong band of low MW DNA.
Example 45: Magentite and polyacrylic acid A suspension of magnetite (iron II, III oxide) between 0.01 and 10~m in diameter was mixed with an excess of polyacrylic acid Mr 240K. After washing, the particles were treated with poly Bis-Tris to promote binding and release of DNA. The composite particles were placed in DNA at 50ug/ml pH4 and after washing at low pH, the DNA
could be eluted with lOmM Tris HCl pH8.5.
Example 46: Magnetic beads with carboxyl groups and Neutral Red A COOH magnetic bead was washed in an excess of 0.lmM
Neutral Red (pKa 6.7) at pH 4. Then the beads were placed in a solution of pure DNA at 50~g/ml pH4 to test whether the DNA would bind to the positively charged dye on the beads. Elution at pH8.5 indicated the had bound to the DNA and released it as the pH was changed.
Example 47: DNA analysis In all previous examples, extracted DNA was analysed by one or more of the following:
(1): ultra violet (UV) analysis at 260nm and 280nm, to provide a measure of nucleic acid concentration;
(2): Gel electrophoresis using to agarose in TBE
buffer run at 60V for 20 minutes vs a commercial preparation of DNA as control, with ethidium laromide staining to measure molecular sire and to provide an estimate of quantity of the nucleic acid; or (3): PCR using primers specific for actin or other ubiquitous genes, to test integrity of the nucleic acid.
The results are presented as (l): direct readings from the instrument; and (2): and (3): gel pictures.
In all cases, the examples demonstrated effective extraction of nucleic acid which was not significantly damaged.
Many standard, weakly basic, buffers are ideal chemical species to provide the ionisable groups of charge switch materials, as they have pKa values close to neutral (i.e.
7) .
for use as a charge switch material, chemical species comprising ionisable groups can be immobilised onto solid supports (e. g. beads, particles, tubes, wells, probes, dipsticks, pipette tips, slides, fibers, membranes, papers, celluloses, agaroses, glass or plastics) in a monomeric or polymeric form via adsorption, ionic or covalent interactions, or by covalent attachment to a polymer backbone which is in turn immobilised onto the solid support. Alternatively, they can be incorporated into solid, insoluble forms (with or without attachment to a polymer backbone) which inherently exhibit charge switching, e.g. beads, particles, tubes, wells, probes, dipsticks, pipette tips, slides, fibers, membranes or plastics.
Solid phase materials, especially beads and particles, may be magnetisable, magnetic or paramagnetic. This can aid removal of the solid phase from a solution containing the released nucleic acid, prior to further processing or storage of the nucleic acid:
Preferably the weakly basic buffers are biological buffers, i.e. buffers from the class of buffers oommonly used in biological buffer solutions. Examples of biological buffers may be found in commercial chemical catalogues, such as the Sigma catalogue.
Leaching (i.e. transfer from the solid phase into solution in the liquid phase) of chemical species used to provide ionisable groups in ion exchange resins is a virtually inevitable phenomenon to some extent, especially when the species are attached to the solid phase by adsorption. Such leaching typically causes impurity in the resultant product, which can lead to significant problems, particularly if the resultant product is intended to be used in PCR (and especially when the species are charged). The use of biological buffers to provide the ionisable groups in charge switch materials can avoid this problem, since leaching of such buffers into the liquid phase will generally not significantly affect the nucleic acid, nor any downstream processes such as PCR to which it might be subjected.
Indeed, many biological buffers are routinely used in PCR
buffers, storage buffers and other buffer solutions.
In a particularly preferred embodiment, the releasing step takes place in a buffer solution containing the same biological buffer that is used in, as or on the charge switch material.
Examples of suitable biological buffers for use in charge switch materials in accordance with the invention, and their pKa values, are as follows:
N-2-acetamido-2-aminoethanesulfonic acid ~~ (ACES), pICa 6. 8;
N-2-acetamido-2-iminodiacetic acid $$ (ADA), pICa 6.6;
amino methyl propanediol t (AMP), pKa 8.8;
3-1,1-dimethyl-2-hydroxyethylamino-2-hydroxy propanesulfonic acid t (AMPSO), pKa 9.0;
N,N-bis2-hydroxyethyl-2-aminoethanesulfonic acid tt (BES), pKa 7.1;
N,N-bis-2-hydroxyethylglycine t (BICINE), pKa 8.3;
bis-2-hydroxyethyliminotrishydroxymethylmethane $$
(Bis-Tris), pKa 6.5;
1,3-bistrishydroxymethylmethylaminopropane $$ (BIS-TRIS Propane), pKa 6.8;
4-cyclohexylamino-1-butane sulfonic acid (CABS), pKa 10.7;
3-cyclohexylamino-1-propane sulfonic acid (CAPS), pKa 10.4;
3-cyclohexylamino-2-hydroxy-1-propane sulfonic acid (CAPSO), pKa 9.6;
2-N-cyclohexylaminoethanesulfonic acid (CHES) pKa 9.6;
3-N,N-bis-2-hydroxyethylamino-2-hydroxypropanesulfonic acid tt (DIPSO), pICa 7.6;
N-2-hydroxyethylpiperazine-N-3-propanesulfonic acid tt (EPPS or HEPPS), pKa 8.0;
N-2-hydroxyethylpipera~ine-N-4-butanesulfonic acid t (HEPBS), pKa 8.3;
N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid tt (HEPES), pKa 7.5;
N-2-hydroxyethylpiperazine-N-2-propanesulfonic acid tt (HEPPSO), pKa 7.8;
2-N-morpholinoethanesulfonic acid $ (MES), pKa 6.1;
4-N-morpholinobutanesulfonic acid tt (MOBS), pKa 7 . 6;
3-N-morpholinopropanesulfonic acid tt (MOPS), pKa 7.2;
3-N-morpholino-2-hydroxypropanesulfonic acid $$
(MOPSO), pKa 6.9;
piperazine-N-N-bis-2-ethanesulfonic acid $t (PIPES), pKa 6. 8;
piperazine-N-N-bis-2-hydroxypropanesulfonic acid tt (P0PS0), pKa 7.8;
N-trishydroxymethyl-methyl-4-aminobutanesulfonic acid t (TABS), pKA 8.9;
N-trishydroxymethyl-methyl-3-aminopropanesulfonic acid tt (TAPS), pKa 8.4;
3-N-trishydroxymethyl-methylamino-2-hydroxypropanesulfonic acid tt (TAPSO), pECa 7.4;
N-trishydroxymethyl-methyl-2-aminoethanesulfonic acid tt (TES), pKa 7.4;
N-trishydroxymethylmethylglycine t (TRICINE), pKa 8.1; and trishydroxymethylaminomethane t (TRIS), pKa 8.1;
histidine*, pKa 6.0, and polyhistidine tt;
imidazole*, pKa 6.9, and derivatives* thereof (i.e.
imidazoles), especially derivatives containing hydroxyl groups**;
triethanolamine dimers**, oligomers** and polymers**; and di/tri/oligo amino acids**, for example Gly-Gly, pKa 8.2; and Ser-Ser, Gly-Gly-Gly, and Ser-Gly, the latter three having pKa values in the range 7-9.
In a preferred embodiment, the buffers marked above with an asterisk (*) are not considered to be biological buffers for the purposes of the invention (whether or not they are designated as such in any chemical catalogue).
In a more preferred embodiment, those marked with two asterisks (**) are also not considered to be biological buffers. Preferred biological buffers are marked with a dagger (t), more preferred buffers are marked with two daggers (tt), still more preferred buffers are marked with a double dagger ($) and most preferred buffers are marked with two double daggers ($~).
These and other chemical species comprising ionisable groups may be coated as monomers onto a solid phase support using covalent, ionic or adsorption interactions.
Additionally or alternatively, they may be coated onto such solid phase supports in polymeric form (preferably following condensation polymerisation), for example by adsorption onto a negatively charged surface (e.g. a surface having exposed COOH or S03 groups), or by covalent attachment. Additionally or alternatively, the chemical species containing ionisable groups may be attached to a polymer (see below) which is then attached to a solid support, e.g. by adsorption or covalent attachment.
Preferably the chemical species or polymer backbones are covalently coupled to the solid support via a hydroxyl group or other group so that the ionisable group having the desired pKa value (usually, but not limited to, a nitrogen atom) remains capable of binding and releasing nucleic acid.
Biological buffers and other chemical species comprising positively ionisable groups may be used in conjunction with a chemical species containing a negatively ionisable group which has a suitable pKa, preferably in the ranges described above. For example a biological buffer (having one or more positively ionisable nitrogen atoms) may be attached to a polymer or other solid phase material which has exposed carboxy groups even after attachment of the biological buffer. Such a material may bind nucleic acids at a low pH when few of the carboxy groups are negatively charged (i.e. few are in the COO- form, most being in the COOH form) and most of the ionisable nitrogen atoms are positively charged. At higher pH the negative charge is stronger (i.e. a greater proportion of carboxy groups are in the COO- form) and/or the positive charge is weaker, and the nucleic acid is repelled from the solid phase.
Chemical species containing ionisable groups (such as the biological buffers listed above) can be attached to a polymer backbone using known chemistries. For example a chemical species containing a hydroxyl group can be attached using carbodiimide chemistry to a carboxylated polymer backbones. Other chemistries include can be employed by someone skilled in the art using other polymer backbones (e. g. based on polyethylene glycol (PEG) or carbohydrate) using a range of standard coupling chemistries (see e.g. Immobilised Affinity Zigand Techniques, Greg T. Hermanson, A. Krishna Mallia and Paul K. Smith, Academic Press, Inc., San Diego, CA, 1992, ISBN 0123423309, which is incorporated herein by reference in its entirety.) Alternatively, the chemical species containing ionisable groups can be polymerised without a backbone polymer, using~cross-linking agents, for example reagents that couple via a hydroxy group (e. g. carbonyldiimidazole, butanediol diglycidyl ether, dialdehydes, diisothiocyanates). Polymers may also be formed by simple condensation chemistries to generate polymeric amino acids with the appropriate pKa e.g. Gly-Gly.
Preferably such immobilisation, attachment and/or polymerisation of the chemical species containing the ionisable group does not affect the pKa of the ionisable group, or leaves it in the desired ranges given above.
For example it is generally preferred not to couple or polymerise the chemical species via a positively ionisable nitrogen atom (in constrast for example to W097/2982). In the practice of the invention, it is especially preferred to immobilise, attach and/or polymerise the chemical species via an hydroxyl group.
A preferred polymeric material is a dimer or oligomer of Bis-Tris, or a material formed by attaching a plurality of Bis-Tris molecules to a polyacrylic acid backbone, e.g. by reacting Bis-Tris monomer with polyacrylic acid using 1-ethyl-3-dimethylaminopropyl carbodiimide (EDC).
The polymer can then be easily separated from the reactants using dialysis against a suitable reagent or water. Preferably the polyacrylic acid has molecular weight of between about 500 and 5 million or more. More preferably it has a molecular weight of between 100,000 and 500,000.
The nature of the resultant Bis-Tris/polyacrylic acid molecule will depend on the ratio of the coupled components, since the polymer will have different properties depending on the proportion of the acrylic acid groups that are modified with Bis-Tris, for example it is desirable for some carboxy groups to remain unmodified, as the presence of these will not prevent the Bis-Tris from binding nucleic acid at low pH (especially if the Bis-Tris is in excess), but their negative charge at higher pHs will assist with release of the nucleic acid. For use in the present invention, the molar ratio of Bis-Tris:carboxy groups (before attachment) is preferably between 5:1 and 1:5, more preferably between 3:1 and 1:3, still more preferably between 2:1 and 1:2, further preferably between 1.5:1 and 1:1.5, and most preferably about 1:1.
The presence of high residual charge (i.e. charged species present in solution along with the extracted nucleic acid) may adversely affect the analysis of nucleic acids by PCR, or interfere with the binding of primers, dNTPs or polymerase to the nucleic acid, or to the sequestration of Mg2+ ions, which are essential to PCR. It is particularly preferable to avoid residual positive charge.
Preferred materials for use in the invention, such as the biological buffers described above, possess minimal residual positive charge (preferably minimal residual charge) at the pH at which the nucleic acid is released, and/or at pHs 8-8.5, making interference with or inhibition of downstream processes unlikely.
Patent application PCT/GB00/02211, of the same inventor, discloses certain methods within the scope of the present invention and is incorporated herein by reference in its entirety as exemplification of the present invention (in all its aspects - see below for other aspects of the invention). In particular, it discloses a method for the extraction of biomolecules from biological material which method comprises contacting the biological material with a solid phase which incorporates histidine or a polyhistidine which will tend to bind nucleic acids at low pH and then extracting the biomolecules bound to the solid phase by elution using an elution solvent which will then release the bound nucleic acids when the pH is increased.
An alternative embodiment of the present invention uses a material which is positively charged across a wide pH
range, such as 0-12 or 0-14 (e. g. an electropositive substance such as a metal oxide, metal, strong or weak base, which lacks a pICa value, or for which the pFCa value is at an extreme of high pH. Such a positively charged material is combined with negatively ionisable material having a pKa intermediate between the pH values at which it is desired to bind and release nucleic acid, or slightly below the pH at which it is desired to bind nucleic acid. This combination of materials allows nucleic acid to be bound at certain pH values, around and below the pKa of the negatively ionisable material, when there are fewer negatively charged groups, but allows the nucleic acid to be released when the pH is increased and a greater number of the ionisable groups are negatively charged. For example, the combination of iron II, III
oxide and polycarboxylates (see Examples) binds nucleic acid at pH 4, when a relative scarcity of negative charges allowing the positively charged iron oxides to bind the nucleic acid. When the pH is increased to around 8, a large proportion of the carboxy groups become negatively charged and, despite the remaining presence of positive charges on the iron oxides, the reduction in overall positive charge allows the nucleic acid to be released.
Further examples of charge switching molecules for nucleic acid purification are based on detergents or surfactants that have a hydrophobic portion and a hydrophilic portion which comprises a positively ionisable group with a suitable pKa, e.g. decyl methyl imidazole or dodecyl-Bis-Tris. These detergents/surfactants can be adsorbed onto surfaces e.g.
plastic via their hydrophobic portions and the hydrophilic (ionisable) portions can be used to capture nucleic acid.
Another family of suitable materials for capture and easy release of nucleic acids are carbohydrates e.g.
glucosamine, polyglucosamine (including chitosans), kanamycins and their derivatives, i.e. sugar ring based structures containing one or more nitrogen atoms surrounded by hydroxyl groups which may also contain other groups such as acetate or sulphate groups to provide a suitable pKa for binding and release of nucleic acids.
Another group of materials with suitable pKa values are nucleic acid bases, e.g. cytidine (pKa 4.2). These can be immobilised via hydroxy groups to a polymer or solid phase carboxy group using carbodiimides.
A still further group of materials having members with suitable pKa values are heterocyclic nitrogen-containing compounds. Such compounds may be aromatic or aliphatic and may be monomers, oligomers or polymers, such as morpholine-, pyrrole-, pyrrolidine-, pyridine-, pyridinol-, pyridone-, pyrroline-, pyrazole-, pyridazine-, pyrazine-, piperidone-, piperidine-, or piperazine-containing compounds, e.g. polyvinylpyridine.
Such compounds may be substituted with electronegative groups to bring the pKa values) of the ionisable nitrogen atoms) into an acceptable range, e.g. as defined above. However, in some compounds this may not be necessary, the pKa already being in such a range.
A still further group of solid phases for binding nucleic acid have surface amine groups, and in particular amine groups which are not polyamines. These monoamine groups can be represented by the formula -NR1R2, where R1 and RZ
are hydrogen or substituted or unsubstituted alkyl.
Although these materials typically have pKa values which at higher than those of materials used in preferred embodiments of the invention, they can be employed in the extracting of nucleic acid, optionally employing them with negatively charged species as described herein to modify the overall pKa of the solid phase.
A further group are materials that provide ionisable groups capable of acting as charge switch materials and binding nucleic acid are dyes, and in particular biological dyes having pKas between 5 and 8. These materials can be immobilised or coated on solid phases.
An example of a biological dye is Neutral Red used in Example 46 below.
Preferred materials for use in accordance with the invention are hydrophilic, for example comprising charge switch materials which are (or which comprise chemical species which before immobilisation or polymerisation are) water soluble.
Once a suitable solid phase has been prepared, comprising a charge switch material, repeated capture and release of nucleic acids can be performed by adjusting the pH up or down. Thus sequential reactions or analysis can be performed on the nucleic acids using the same solid phase. For example, DNA can be isolated from a biological sample using a PCR tube comprising a charge switch material. Then, following PCR, the amplified DNA
product may be isolated from the buffer constituents or primers by adjusting the pH in the same tube.
Particularly preferred solid phase materials are non-porous. Porous supports are commonly used for isolating proteins, which can be trapped in the pores of the support. However, nucleic acids tend to be too big to enter into pores of commonly used such supports, and will therefore become bound to the surface of the support, potentially trapping impurities in the pores.
The method can be used to separate single stranded RNA or DNA from double stranded DNA, because of the different charge densities on single and double stranded molecules, by appropriate manipulation of the pH or salt concentration. Typically, single stranded molecules will be released from binding to the solid phase at a lower pH
than double stranded molecules.
In some circumstances, for example for the construction of gene chips, and for the preparation of probes, it may be desirable to produce single stranded DNA.
Manipulation of pH andlor ionic strength can assist in purification and release of single stranded nucleic acid.
The method of the invention may comprise a prior step of converting double stranded nucleic acid in the sample to single stranded nucleic acid (preferably using a strong base, e.g. 100mM NaOH, or a weak base at high temperature, e.g. 60-100°C). The solid phase material is preferably then added simultaneously with a buffer which changes the pH of the sample to the pH for binding single stranded nucleic acid (typically a pH of 4-7).
The materials described herein may also be employed to capture nucleic acids in the liquid phase where binding leads to a cross-linked lattice large enough to separated from the liquid phase, e.g. by filtration or centrifugation.
Accordingly, in a second aspect, the present invention provides a method for extracting nucleic acid from a sample containing nucleic acids, which method comprises:
contacting the sample with a charge switch material at a first pH at which the charge switch material has a positive charge and will bind negatively charged nucleic acid; and then releasing the nucleic acid at a second, higher pH at which the charge is neutral, negative or less positive than at the first pH, wherein the charge switch material is soluble at said first pH, and wherein the combination of the charge switch material and the bound nucleic acid is insoluble at or above said first pH
and below said second pH.
Preferred features of the method are as set out above, with the exception of the charge switch material being formed into, immobilised on, or attached to, a solid phase material.
Usually the charge switch materials will be soluble at the second pH, and will remain in solution with the nucleic acid upon release of the nucleic acid; the use of a weakly basic buffer (optionally bound to a soluble backbone, e.g. polyacrylic acid) as the charge switch material can avoid problems of contamination as described above.
The methods of the invention preferably include one or more washing steps between the binding and releasing steps. Such (a) washing steps) will generally be carried out at said first pH, or a pH above said first pH
but lower then said second pH, such that the nucleic acid is substantially not released during the washing step(s).
As has been indicated previously, the methods of the invention are particularly suitable for extracting nucleic acid which is then stored or further processed (e. g. by PCR), particularly when the charge switch material is in the form of e.g. a tube or well in which such storage and/or processing can occur. For the avoidance of doubt, however, it is emphasised that the releasing step and any subsequent storage or processing need not be carried out as discrete steps, but can coincide, when said storage or processing occurs at a pH
at which release of the nucleic acid occurs. For example, the method of the invention includes binding nucleic acid to a charge switch material coated on or otherwise provided lay a PCR tube, washing the bound nucleic acid, and then without a separate releasing step commencing the PCR reaction using a PCR buffer which causes release of the nucleic acid.
In a further aspect, the present invention provides novel charge switch materials for use in the methods of the receding aspects. It further comprises the use of such charge switch materials in such methods. All preferred features of the charge switch materials described in above in the context of the methods apply equally and independently to the present aspect of the invention (i.e. preferred combinations of features may be different in relation to this aspect from the preferred combinations in relation to the method aspects).
In a further aspect, the present invention provides a container (preferably a PCR or storage tube or well, or a pipette tip) coated with, comprising or formed from a charge switch material, preferably a charge switch material comprising a biological buffer.
The following description is directed particularly to the extraction of nucleic acid from blood, but applies also to the extraction of nucleic acid from any liquid sample, particularly biological samples or samples produced during laboratory techniques, such as PCR.
The method is particularly useful if the biological material is blood, but the method can be used for a range of applications substances such as plasmid and vector isolation and plant DIVA extraction.
Preferably the cells in the blood are lysed to release nucleic acids and known lysing agents and methods can be used, such as contacting with ionic and non ionic detergents, hypotonic solutions of salts, proteases, chaotropic agents, solvents, using pH changes or heat. A
method of lysing cells to isolate nucleic acid is described in WO 96/00228.
When the biological material consists of blood the samples can optionally be diluted with water or other diluent in order to make it easier to manipulate and to process.
Dilutions up to ten times can be used and in general more dilution can be better and it is a feature of the present invention that it allows low dilution of blood to be possible.
The solid phase with which the blood is contacted, can be a formed of a material which has a natural affinity for nucleic acids or it can be formed of a material which has its surface treated with an agent which will cause nucleic acids to bind to it or increase its affinity for nucleic acids. Suitable materials include controlled pore glass, polysaccharide (agarose or cellulose), other types of silica/glass, ceramic materials, porous plastic materials such as porous plastic plugs which in a single moulded part or as an insert in a standard tube, polystyrene beads para magnetic beads etc. The size and porosity is not critical and can vary and be selected for particular applications.
Suitable means for treating the surface of the solid phase or for derivatising it include treating it with a substance which can introduce a charge e.g. a positive charge on the surface or a hydrophilic or hydrophobic surface on the solid phase e.g. hydroxyl groups, nitrate groups, autoreactive groups, dyes and other aromatic compounds.
In a preferred embodiment of the invention the solid phase will cause DNA to be bound to it at one pH in preference to contaminants in the blood sample and will allow the bound nucleic acid to be released when it is contacted with an eluant at a different pH. This system can be used with a solid phase which incorporates histidine or a polyhistidine which will tend to bind nucleic acids at low pH e.g. less than 6 and will then release the bound nucleic acids when the pH is increased e.g. to greater than 8. Alternatively the nucleic acids are bound at substantially neutral pH to an aminated surface and released at very high pH.
In another embodiment of the invention a plastic moulding can incorporate a binding agent e.g. in a well in a plate etc. so that the binding agent is incorporated in the surface, the blood sample is then contacted with the surface so as to cause nucleic acids to be bound to the surface. The blood sample is then removed and the surface treated with an eluting agent to release the bound nucleic acids. When the surface is part of a well in a multi- well plate, the total system can be readily adapted for rapid large scale sampling and extraction techniques, Binding agents which can be used include charge switchable ion exchange resins using a positively charged solid phase that can be reversed or made neutral by changing the pH above its pKa. e.g. nucleotides, polyamines, imidazole groups and other similar reagents with a suitable pKa value.
Also, nucleic acids can be bound by intercalation using a variety of intercalating compounds incorporated into the solid phase e.g. actinomycin D, ethidium bromide etc.
In a further embodiment of the invention a plastic surface can be modified to include functional groups. The plastic can be any plastic used for containing samples e.g. polypropylene. The functional groups can be positively or negatively charged so as to bind the nucleic acids in the correct buffer solution.
Alternatively the functional groups can be chemical groups capable of covalent coupling to other ligands or polymers.
When the plastic is used in a plastic moulding e.g. in a well in a plate, or as a polymerase chain reaction (PCR) tube, the surface characteristics of the plastic can be suitably modified for use in the present invention by including or adding the appropriate chemicals in the moulding compound e.g. as in an injection moulding compound.
When this is used in a PCR tube or in a deep well plate the tubes or wells can be used to isolate and immobilise small quantities of DNA or RNA generating a pure template for subsequent PCR or other genetic analysis and manipulation.
When the plastic is polypropylene e.g. it is in the form of a thin walled PCR tube the polypropylene surface can be modified by oxidising the surface with an oxidising agent such as potassium permanganate and sulphuric acid to create a carboxylated surface (C00H groups). This tube can then be used to improve the isolation of DNA from solutions or from crude samples e.g. blood. By adjusting the pH, di-electric constant, solubility or ionic strength the DNA or RNA can be immobilised on the walls of the tube, washed free of contaminants, ready for PCR
or other analytical techniques.
The carboxy groups can be further modified by covalently coupling an anionic group such as imidazole or polyhistidine or any strong or weak ion exchanger, to allow binding of nucleic acids by a charge interaction.
This tube could then be used to improve the isolation of DIVA from solutions or from crude samples e.g. of blood.
Again by adjusting the pH, di-electric constant, or ionic strength the DNA or RIVA can be immobilised on the walls of the tube, washed free of contaminants, ready for PCR
or other analytical techniques.
The nucleic acids can be eluted with in a low salt buffer so that it is ready for PCR or other analysis.
The solid phase can be contacted with a blood sample by mixing with the solid phase in a mixingl stirring device, by passing the blood sample over the solid phase or the solid phase can be paramagnetic and manipulated by a magnetic field. Although the invention is particularly suitable for the separation or isolation of nucleic acids from blood it can be used with a range of biomolecules particularly those that require removal of cell wall debris or insoluble particles.
In a preferred embodiment of the invention the solid phase is in granular form in a column and the blood sample is drawn up through the column by means of a pressure differential being applied through the column, the blood sample is drawn up with air and the granular solid material can become fluidised thus increasing the mixing and contacting rates and minimising clogging.
The method of the invention is suitable for use in a multi-well format when a series of extractions from different samples can take place substantially simultaneously and this will facilitate the automation of the extraction process allowing rapid high throughput extraction to take place and to allow combinational chemistry to be performed. This will enable there to be a high throughput in a standard well array e.g. an eight by twelve array so that a large number of sample types can be treated automatically at the same time.
Detailed Description The invention, in its various aspects, will now be described in detail, by way of example only.
Example 1: Extraction of nucleic acids from whole blood A charge switchable ion-exchanger was prepared by covalently coupling polyhistidine to 100 um glass beads using glutaldehyde by mixing 1 gram of the aminated glass beads with 0.01 o(v/v) glutaldehyde in 0.1M sodium bicarbonate at pH8 containing 20mg polyhistidine. After overnight incubation the beads were washed exhaustively to remove non-covalently bound material and stored in lOmM MES, pH5 containing 0.1 0 (v/v) Tween 20.
About 300mg of the 100pm derivatised glass beads were added to a lml plastic column enclosed at both ends.
A blood sample was incubated with an equal volume of lOmM
MES pH5, containing 1o Tween 20, proteases (200ug/ml) and 1 mM EDTA. After digestion is complete the blood was sucked up the column containing the glass beads and the DNA became immobilised allowing the contaminating proteins to pass through to waste.
The glass beads containing the immobilised DNA were washed with a buffer comprising lOmM MES pH5, containing 1o Tween 20, and 1 mM EDTA and this was repeated until the wash solution was colourless.
After washing, the beads were dried with air and DNA
eluted with a small quantity of lOmM Tris HCl, pH 8.5 and collected in a sterile tube ready for analysis. Thus the DNA were separated from the blood. For different biomolecules, the buffer etc. can be suitably modified.
Example 2:Polyhistidine on carboxylated beads One gram of carboxylated paramagnetic beads were washed in 50mM imidazole buffer pH6 and then mixed with 100mg of polyhistidine in 50m1 of 50mM Imidazole buffer pH 6. A
chemical coupling agent was added (EDC) at a final concentration of 5mg per ml and mixed overnight. The beads were washed in water, 0.5M sodium chloride, 10 Tween 20, 100mM Tris HCl pH 8 and stored in lOmM MES, 0.1o Tween 20 pH5.
To extract DNA from blood, lmg of beads were mixed with blood diluted in 10o Tween 20 with 25mM MES, 1mM EDTA pH
5. The beads were separated with a magnet and washed by resuspending in 1mM MES, 0.1o Tween 20. To elute the DNA
the beads were resuspended in lOmM Tris HCl pH 8.5 and separated with magnet leaving the DNA in solution.
Example 3: Bis-Tris solid phase magnetic beads 200mg of carboxylated 1 ~,m magnetic particles were reacted in a one step procedure with 100mg of Bis-Tris and 100mg of the carbodiimide, EDC, in 50mM imidazole buffer pH6Ø Following an overnight incubation, the magnetic particles were washed and used to isolate Plasmid DNA.
An alkaline lysis method was used to prepare a cleared 5m1 bacterial lysate generating a supernatant containing the plasmid in 0.5M potassium acetate, pH5. To the supernatant, 2.5mg of magnetic particles were added and mixed for 1 minute. After magnetic separation and washing with water pHS, the pure plasmid DNA was eluted off in 2001 of lOmM Tris.HCl pH 8.5.
The magnetic beads were also used to extract DNA directly from whole blood using a detergent based digestion reagent containing proteinase K.
Example 4: Tricine on solid phase magnetic beads 50mg of carboxylated 1 ~,m magnetic particles were reacted in a one step procedure with 50mg of Tricine and 100mg of the carbodiimide, EDC, in 50mM imidazole buffer pH6Ø
Following an overnight incubation, the magnetic particles were washed and used to isolate plasmid DNA. An alkaline lysis method was used to prepare a cleared 5m1 bacterial lysate generating a supernatant containing the plasmid in 0.5M potassium acetate, pH5. To the supernatant, 2.5mg of magnetic particles were added and mixed for 1 minute.
After magnetic separation and washing with water pH5, the pure nucleic acids were eluted off in 200.1 of lOmM
Tris.HCl pH 8.5.
Example 5: Bis-Tris solid phase polystyrene beads 1 gram of carboxylated 60~,m polystyrene particles were reacted in a one step procedure with 500mg of Bis-Tris and 500mg of the carbodiimide, EDC, in 50mM imidazole buffer pH6Ø Following an overnight incubation, the particles were washed and used to isolate plasmid nucleic acids as described above.
Example 6: Bis-Tris polymer Bis-Tris monomer was converted into a polymer by mixing together 160mg of polyacrylic acid with a molecular weight of 240,000, 1.6g of Bis-Tris and 1.6g of EDC in 50mM imidazole pH6Ø Following an overnight incubation, the mixture was dialysed in water. The purified polymer was then coated onto magnetic COOH beads or used in the liquid phase to bind genomic DNA from blood. A 5m1 blood sample was centrifuged to obtain the nuclei and WBC
population and the resulting pellet digested with 1o SDS.
Following precipitation with potassium acetate the cleared supernatant was mixed with either 25mg of magnetic-Bis-Tris or about 250~g of poly-Bis-Tris as a liquid. In both cases the captured DNA could be separated, washed in water and then redissolved in lOmM
Tris HC1 pH8.5 in a pure form.
Example 7: Insoluble Tris HC1 polymer In this example an insoluble polymer was made with inherent charge switching properties by mixing 80mg of polyacrylic acid with 800mg of Tris HC1 and 800mg of EDC
in 50mM Imidazole pH6. The insoluble precipitate that formed generated a particulate solid phase that was used to capture DNA and release it in a similar manner to that described in Example 4 for genomic DNA.
Example 8: Immobilised poly Bis-Tris on pipette ti~
A solution of poly Bis-Tris at 1mg/ml, prepared as in Example 2, in O.1M sodium bicarbonate pH8 incubated at 60°C for 8 hours with twenty 200~Z1 polyproplylene pipette tips. The tips were then rinsed and used to capture about 150ng of plasmid DNA from a cleared bacterial lysate by pumping up and down ten times. After a quick wash with water pHS, the DNA was eluted in 50,1 of lOmM
Tris pH 8.5.
Example 9: Immobilised poly Bis-Tris on PCR tubes A solution of poly Bis-Tris at 1mg/ml, prepared as in Example 2, in 0.1M sodium bicarbonate pH8 incubated at 60°C for 8 hours in a 200u1 PCR plate of 8x12 tubes.
After rinsing, the tubes were used to bind genomic DNA
from a sample prepared according to Example 4. About 50ng of DNA was subsequently eluted off per tube using lOmM Tris HCl pH8.5.
Example 10: Charge switch detergents in liquid phase A blood sample was prepared as described in Example 4 and to the resulting supernatant decyl imidazole was added at pH 4 causing precipitation of the DNA. The DNA pellet was collected by centrifugation and redissolved in lOmM
Tris pH 8.5.
Example 11: Charae switch detergents on solid t~hase Decyl imidazole was adsorbed onto a 200u1 plastic pipette tip by soaking in a 1o solution at pH4 in 0.1M sodium acetate. A blood sample was prepared as described in Example 3 and the tips were used to bind the DNA by repeated pumping and sucking. After a wash with water, about 50ng of DNA was recovered in water at pHlO.
Example 12: Polyglucosamines l0mg of low molecular weight Chitosan was dissolved in acidified water and then 50mM imidazole pH5.5, this was mixed with 100mg of carboxy 1 ~,m magnetic beads and with 20mg of the carbodiimide EDC in 50mM imidazole pH5.5.
Following an overnight incubation, the beads were washed and resuspended in lOmM MES pH5. To bind genomic DNA, 2mg of magnetic particles were added to a supernatant prepared by methods described earlier in Example 1, after magnetic separation, the DNA was eluted using 100mM
Tris.HCl pH 9.5.
Example 13: Kanamycin A solution of genomic DNA was prepared as described in example 3. To this sample 2mg of Kanmycin was added at a concentration of l0mg/ml. The resulting precipitate of DNA was filtered, washed in water at pH5 and re-dissolved in water at pHlO.
Example 14: Magnetisable iron oxides in carboxylated polystrene A 5m1 plasmid mini-prep was prepared using standard alkaline lysis reagents to generate a cleared lysate with a potassium acetate composition of 0.5M pH4. To this cleared supernatant, 2.5mg of commercially available l~,m carboxylated polystyrene magnetisable particles were added to bind the plasmid DNA. The particles were washed with water at pH4 and then the DNA eluted using lOmM Tris HC1 at pH 8.5. Typical UV ratios at 260 and 280nm were 1.7-2.0, indicating pure nucleic acids with a single band observed with standard gel electrophoresis.
Example 15: Titanium dioxide in polystyrene microtitre l~ ates A solution of DNA at 100~,g per ml in 0.1M Potassium Acetate pH4 was allowed to stand for 1 hour in a 300u1 flat bottomed microtitre plastic plate, the plastic plate contained titanium oxide which was incorporated as a powder in the plastic when the plate was formed. After washing at pH4, the DNA was recovered with water at pHlO
and 2m1 measured at 260nm versus a plain polystyrene plate with no titanium oxide. Approximately, 50ng of DNA
was recovered per 300u1 well for the plate incorporating the titanium oxide compared to zero for the plain polystyrene plate.
Example 16: Cytidine coupled to magnetic beads 1 gram of carboxylated 1 ~m magnetic particles were reacted in a one step procedure with 500mg of Cytidine and 500mg of the carbodiimide, EDC, in 50mM imidazole buffer pH6Ø After thorough washing, the beads were used to bind nucleic acids from a plasmid preparation as described in example 1 and recovering the pure nucleic acids in water at pHlO.
Example 17: Polyvinyl pyridine (PVP) 20mg of commercially available PVP beads was mixed with the supernatant containing genomic DNA from a 5m1 blood extraction described in Example 4. After allowing the DNA to bind, the beads were washed with water at pH5 and the DNA recovered using water at pHlO. Ultra violet analyisis at 260 and 280nm indicated a purity ratio of 1.65.
Example 18: Separation of RNA and DNA
A solution of tRNA and sheared genomic DNA was prepared at 30~,g per ml in 50mM Potassium acetate buffer pH6.5 with 1M sodium chloride. Approximately 4mg of magnetic polyhistidine beads were mixed with 1m1 of the nucleic acid solution for one minute until binding was complete.
The beads were then thoroughly washed with water at pH5.
To elute the bound material, the beads were mixed with 300,1 of lOmM Tris.HCl, lOmM NaCl, pH8.5. Gel analysis showed that most of the tRNA remained in solution and was not bound to the beads. The eluted material contained mostly genomic DNA with little or no tRNA.
Example 19: PCR tubes with surface amine groups PCR tubes were modified to provide surface amine groups for binding DNA or RNA. About 2001 of supernatant from a cleared lysate from a pUCl9 plasmid prep. was allowed to incubate in the tube for 10 minutes. The tube was then washed with water and the bound DNA on the walls of the tube was treated with a PCR reaction mixture with primers for pUCl9. After the PCR reaction mixture was completed, the tubes contents were run on a 1o gel with ethidium bromide. The gel showed a strong band indicating that the PCR reaction had been successful.
Example 20: Magnetic beads With surface amine groups Commercially available 4um magnetic beads with surface amine groups for binding DNA or RNA were mixed with about 2001 of supernatant from a cleared lysate from a PU19 plasmid prep. and allowed to incubate in the tube for 10 minutes. Then 5u1 beads (about 100~g of beads) were washed with water and the beads added to a PCR reaction mixture with primers for pUCl9. After the PCR reaction mixture was completed, the mixture was run on a 1o gel with ethidium bromide. The gel showed a strong band indicating that the PCR reaction had been successful.
Example 21: Magnetic beads with surface amine groups Commercially available 4um magnetic beads with surface amine groups for binding DNA or RNA were mixed with about 200p1 of supernatant from a cleared lysate from a pUCl9 plasmid prep. and allowed to incubate in the tube for 10 minutes. Then 5u1 beads (about 100~g of beads) were washed with water and the beads mixed in 100u1 of water at pH 10 using NaOH to elute enough DNA for a PCR
reaction . About 10p1 of the eluted DNA was mixed with a PCR master mix with primers for PU19. After the PCR
reaction mixture was completed, the mixture was run on a 1% gel with ethidium bromide. The gel showed a strong band indicating that the PCR reaction had been successful.
l0 Example 22: Magnetic beads with surface amine groups Commercially available 4~Zm magnetic beads with surface amine groups for binding DNA ox RNA were mixed with about 200u1 of supernatant from a cleared lysate from a pUCl9 plasmid prep. and allowed to incubate in the tube for l0 minutes. Then 5~1 beads (about 100ug of beads) were washed with water and the beads mixed in 100u1 of a 10 times strength PCR buffer to elute enough DNA for a PCR
reaction. About 5~Z1 of the eluted DNA was mixed with a 45u1 of water with primers for pUCl9. After the PCR
reaction mixture was completed, the mixture was run on a 1o gel with ethidium bromide. The gel showed a strong band indicating that the PCR reaction had been successful.
Example 23: Polyacrylic acid-diamine ethylene mixed charge polymer A mixed charge polymer for binding DNA or RNA was prepared by mixing 100mg of polyacrylic acid Mr 240K with 20mg of diamine-ethylene in 50m1 of 0.1M imidazole buffer pH 6.5 and adding 300mg of EDC cross linking agent.
After overnight incubation the mixture was dialyzed exhaustively and used to bind DNA by adding 100~z1 to a white blood cell digest containing 7M Urea at pH 5. The resulting complex of DNA and polymer was collected by centrifugation, washed in water at pH 5 and re-dissolved in water at pH 10.
Example 24: Polyacrylic acid-triethanolamine mixed charge polymer A polymer for binding DNA or RNA was prepared by mixing 100mg of polyacrylic acid Mr 240K with 1g of Triethanolamine in 50m1 of 0.1M imidazole buffer pH6.5 and adding 1g of EDC cross linking agent. After overnight incubation the mixture was dialyzed exhaustively and used to bind DNA by adding 1001 to a white blood cell digest containing 7M Urea at pH 5. The resulting complex of DNA and polymer was collected by centrifugation, washed in water at pH 5 and re-dissolved in water at pH 10.
Example 25: Polyacrylic acid-Bis-Tris mixed charge polymer A mixed charge polymer for binding DNA or RNA was prepared by mixing 100mg of polyacrylic acid Mr 240K with 20mg of Bis-Tris in 50m1 of O.1M imidazole buffer pH 6.5 and adding 300mg of EDC cross linking agent. After overnight incubation the mixture was dialyzed exhaustively and used to bind DNA by adding 1001 to a white blood cell digest containing 7M Urea at pH 5. The resulting complex of DNA and polymer was collected by centrifugation, washed in water at pH 5 and re-dissolved in lOmM Tris HC1 pH 8.5.
Example 26: Carboxyl groups magnetic beads and Bis-Tris A mixed charge polymer of C00H and Bis-Tris as previously described was coupled to a COOH magnetic beads by adding 100mg of beads with l0mg of polymer and 20mg of EDC in O.1M imidazole at pH6. The beads showed significant binding of purified DNA at pH 5 and release in lOmM Tris HCl pH 8.5.
Example 27: Polyacrylic acid and poly Bis-Tris A soluble polymer of poly Bis-Tris was formed in-situ inside a porous plastic plug by mixing polyacrylic acid with Bis Tris in the ratio of 1:10 in 0.1M imidazole buffer pH6.5. Then EDC was added in a 10 fold excess to polyacrylic acid to form the polymer. This was washed in l6mM potassium acetate pH 4 to remove unbound material and the plug then used to bind DNA from a 7M Urea digest of white blood cells at pH 5. The DNA could be eluted off the plug by increasing the pH to 8.5 using lOmM Tris HCl.
Example 28: Polyacrylic acid and poly Bis-Tris An insoluble polymer of poly Bis-Tris was formed in situ inside a porous plastic plug by mixing polyacrylic acid with Bis Tris in the ratio of 1:10 in O.1M imidazole buffer pH 5.5. Then EDC was added in a 10 fold excess to polyacrylic acid to form the polymer. This was washed in l6mM potassium acetate pH 4 to remove unbound material and the plug then used to bind DNA from a 7M Urea digest of white blood cells at pH 5. The DNA could be eluted off the plug by increasing the pH to 8.5 using IOmM Tris HCl.
Example 29: Magnetic beads derivatised with Bis-Tris A cleared lysate supernatant from an E. coli culture containing pUCl9 plasmids was prepared using standard alkaline lysis reagents. About 501 of the cleared supernatant at pH4 was mixed with 0.25mg of magnetic beads derivatised with Bis-Tris to bind the plasmid DNA.
After washing the impurities away with water, the beads with DNA were then mixed in a PCR reaction in a PCR tube and transferred to a Thermal Cycler for amplification using appropriate primers. Following PCR, the pH was adjusted to 4 using potassium acetate and the PCR product was bound to the same magnetic beads. The beads were washed with water and the clean PCR product was eluted in lOmM Tris HCl pH8.5 for analysis on a gel and by sequencing. The final results showed that the beads could purify the plasmid, then allow PCR in the presence of the beads and finally clean up the PCR product by re-binding and elution of the PCR product ready for detection or further manipulation.
Example 30: Microtitre plate using poly Bis-Tris A carboxylated microtitre plate was prepared by plasma treatment and washed with a solution of poly Bis-Tris at 0.5mg/ml in lOmM potassium acetate pH 5. After washing away unbound material, a solution of blood was added diluted 10 fold in lOmM ammonium carbonate/bicarbonate pH7 with 1o Tween20. After 30 minutes incubation time, the DNA became immobilised on the walls of the wells and the contaminants washed away with water. The DNA was then recovered by mixing with lOmM Tris HC1 pH8.5.
Example 31: Plastic tip using poly Bis-Tris A solution of human DNA at 50ug/ml in 200mM potassium acetate buffer pH5 was mixed with an equal volume of isopropanol. The resulting precipitate was separated using a COOH plastic tip coated with poly Bis-Tris.
After removing all waste liquid, the precipitate of DNA
was re-dissolved in lOmM Tris HCl pH8.5.
Example 32: Sintered disk with poly Bis-Tris Some magnetic beads modified with Bis-Tris were dried in air and then ground using a pestle and mortar. This powder was mixed with some plastic granules and formulated into a sintered disc approximately 3mm thick and 6mm in diameter. The disc could then be used to bind and elute DNA from a solution of calf thymus DNA at 50~g/ml pH4 using lOmM Tris HCl pH8.5 to recover the DNA.
Example 33: Polyhydroxlated amines on silica 1 gram of silica particles of about 60~m in diameter was treated with 10o v/v 3-bis(2-hydroxyethyl)aminopropyl-triethoxy silane in acetone at 60C for l8hours. The modified particles were washed in acetone then water and used for binding calf thymus DNA at 50ug/ml in lOmM
potassium acetate pH4. The DNA was subsequently released at pH 10 in lOmM Tris HC1.
Example 34: Differential binding using magnetic beads with Bis-Tris Differential binding of RNA and DNA from E. coli was demonstrated by preparing cleared bacterial lysates using standard alkaline lysis reagents and ammonium acetate added from 0 to 500mM as a final concentration. About 0.5mg~of Bis-Tris magnetic beads was added to the mixtures to bind the nucleic acids. After washing in water, the beads were eluted in lOmM Tris HC1 pH8.5. The gel results showed that by increasing salt, reduced the amount of RNA bound to the beads relative to DNA, confirmed by measuring the ratio of dsDNA to single stranded DNA/RNA using UV at 260nm and pico green binding.
Example 35: Filter with poly Bis-Tris A 3 micron glass fibre paper filter was soaked in an excess of poly Bis-Tris at 0.5mg/ml in lOmM potassium acetate pH 4 and air dried for 3 days. A 50~g spot of DNA was placed on the filter and dried again so that it may be stored and archived. To recover the DNA, the filter was washed in water and then 2m1 of lOmM Tris HCl pH 8.5 allowed to soak in for 10 minutes and the resulting UV absorbance at 260nm indicated an 800 recovery of DNA.
Example 35: Magnetic beads with Bis-Tris A buccal cell swab was used to collect a sample and placed in digest buffer containing lml of 20mM Ammonium bicarbonate, 1% Tween 20 and proteinase K at 200~g/ml at pH7. After 20 minutes at 50°C, the liquid was transferred to a new tube containing potassium acetate pH
4 and 1mg of magnetic Bis-Tris beads. After a brief incubation the beads were separated with a magnet and washed with water. The DNA or RNA was eluted off using lOmM Tris HCl pH8.5 and visualised on a gel and tested by PCR.
Example 36: Magnetic beads with poly Bis-Tris A 501 blood sample was diluted with a 20 fold excess of 0.010 SDS, lOmM Tris HCl pH 7 and incubated for 5 minutes at room temperature. A magnetic bead coated with poly Bis-Tris was used to capture the DNA and after washing with water it was eluted off using lOmM Tris HCl pH 8.4.
Example 37: Magnetic bead with carboxyl groups and Bis-Tris A magnetic bead was prepared with both COOH and Bis-Tris ligands determined by binding of Congo Red and Neural Red dyes. Purified genomic DNA at 50~g/ml in acetate buffer pH 4 was mixed with lmg of beads and the subsequent complex of bound material was washed and then the DNA
recovered by eluting with 100~z1 of lOmM Tris HCl pH 8.5.
This was repeated with a plasmid extraction from E. Coli containing pUCl9 and pure plasmid was released in the Tris elution buffer as previously described.
Example 38: Silica particles with carboxyl groups and Bis-Tris 1 gram of silica particles of about 60microns in diameter with surface COOH groups were mixed with 1 gram of Bis-Tris in 20m1 of 0.1M imidazole pH6 and 200mg of EDC. The modified particles were washed and used to bind and elute pUCl9 plasmid from a cleared lysate prepared using alkaline lysis reagents.
Example 39: Silica particles with surface amine groups and polyacrylic acid 1 gram of silica particles of about 60 microns in diameter with surface amine groups were mixed with 1 gram of polyacrylic acid Mr 240IC in 20m1 of 0.1M imidazole pH
6 and 200mg of EDC. The modified poly COON particles were washed and were mixed with lgram of Bis-Tris in 20m1 of 0.1M imidazole pH6 and 400mg of EDC. The modified particles were used to bind and elute pUCl9 plasmid from a cleared lysate prepared using alkaline lysis reagents.
Example 40: Differential binding using magnetic Bis-Tris beads Differential binding and elution of RNA and DNA was demonstrated from a plasmid prep. by adjusting the pH for binding near to the pKa of the magnetic Bis-Tris beads.
Alkaline lysis reagents were prepared using acetate Precipitation buffers at pH 4.0, 4.2, 4.4, 4.6, 4.8 so the final pH for binding plasmid was between 4 and 6 after neutralisation. By measuring single stranded RNA
and double stranded DNA after elution from the beads, an estimate of the relative ratios of each was calculated.
In this case, it was found that the RNA binding or elution remained fairly constant while the DNA binding or elution decreased with increasing pH.
Example 41: PCR clean up with magnetic beads with Bis-Tris A PCR clean up reaction was demonstrated by preparing a 600bp and l.SKb PCR reaction for pUCl9 using 401 for purification. To the 40u1 of PCR reaction, 500p1 of about 0.5M acetate buffer pH4 was added and 0.25mg,of Bis-Tris magnetic beads. The beads bound the PCR product and were washed clean with water and eluted in 501 of lOmM Tris HC1 pH 8.5.
Example 42: CMC polymer and Bis-Tris beads A carboxy methyl cellulose polymer, grade 9H4F, was used to prepare a 1o solution in water. The mixture was then added dropwise to a solution of 1M calcium chloride to form a polyelectrolyte complex with surface COOH groups.
The resulting beads were then converted to Bis-Tris beads using an excess of Bis-Tris and EDC in an imidazole buffer pH 6. The beads showed binding and release of pure DNA using pH 4 and pH 8.5 buffers respectively.
Example 43: Insoluble Bis-Tris An insoluble form of poly Bis-Tris was prepared by mixing 160mg of polyacrylic acid Mr 240K, with 1.6 g of Bis Tris in 50m1 0.1M imidazole at pH 5.9 with 1.6 g of EDC. The precipitated polymer was collected by centrifugation and the small particles showed binding and release of DNA at pH 4 and pH 9 respectively.
Example 44: Magnetic beads with Bis-Tris To test whether oligonucleotides could bind to modified magnetic beads, 1mg of Bis-Tris beads were mixed with l0ug of pUCl9 PCR primers in lOmM potassium acetate pH4.
After washing with water pH4, the beads were eluted with lOmM Tris HCl pH8.5 and the UV reading indicated a recovery of 5~g of DNA. A 1o gel containing Sybr green dye also showed a strong band of low MW DNA.
Example 45: Magentite and polyacrylic acid A suspension of magnetite (iron II, III oxide) between 0.01 and 10~m in diameter was mixed with an excess of polyacrylic acid Mr 240K. After washing, the particles were treated with poly Bis-Tris to promote binding and release of DNA. The composite particles were placed in DNA at 50ug/ml pH4 and after washing at low pH, the DNA
could be eluted with lOmM Tris HCl pH8.5.
Example 46: Magnetic beads with carboxyl groups and Neutral Red A COOH magnetic bead was washed in an excess of 0.lmM
Neutral Red (pKa 6.7) at pH 4. Then the beads were placed in a solution of pure DNA at 50~g/ml pH4 to test whether the DNA would bind to the positively charged dye on the beads. Elution at pH8.5 indicated the had bound to the DNA and released it as the pH was changed.
Example 47: DNA analysis In all previous examples, extracted DNA was analysed by one or more of the following:
(1): ultra violet (UV) analysis at 260nm and 280nm, to provide a measure of nucleic acid concentration;
(2): Gel electrophoresis using to agarose in TBE
buffer run at 60V for 20 minutes vs a commercial preparation of DNA as control, with ethidium laromide staining to measure molecular sire and to provide an estimate of quantity of the nucleic acid; or (3): PCR using primers specific for actin or other ubiquitous genes, to test integrity of the nucleic acid.
The results are presented as (l): direct readings from the instrument; and (2): and (3): gel pictures.
In all cases, the examples demonstrated effective extraction of nucleic acid which was not significantly damaged.
Claims
Claims:
1. A method for extracting nucleic acid from a sample containing nucleic acid, which method comprises:
at a first pH, bringing the sample into contact with a material which comprises an ionisable group immobilised on a solid support, wherein the material has a positive charge at said first pH, such that nucleic acid is bound to the material: and releasing the nucleic acid at a second, higher, pH
at which the charge on the material is negative, neutral or less positive, wherein the release of the nucleic acid occurs under mild conditions and the ionisable group is provided by a chemical species selected from:
N-2-acetamido-2-aminoethanesulfonic acid (ACES);
N-2-acetamido-2-iminodiacetic acid (ADA):
amino methyl propanediol (AMP);
3-1,1-dimethyl-2-hydroxyethylamino-2-hydroxy propanesulfonic acid (AMPSO):
N,N-bis2-hydroxyethyl-2-aminoethanesulfonic acid (BES):
N,N-bis-2-hydroxyethylglycine (BICINE);
bis-2-hydroxyethyliminotrishydroxymethylmethane (Bis-Tris);
1,3-bistrishydroxymethylmethylaminopropane (Bis-Tris Propane):
4-cyclohexylamino-1-butane sulfonic acid (CABS);
3-cyclohexylamino-1-propane sulfonic acid (CAPS);
3-cyclohexylamino-2-hydroxy-1-propane sulfonic acid (CAPSO);
2-N-cyclohexylaminoethanesulfonic acid (CHES);
3-N,N-bis-2-hydroxyethylamino-2-hydroxypropanesulfonic acid (D.tau.PSO);
N-2-hydroxyethylpiperazine-N-3-propanesulfonic acid (EPPS);
N-2-hydroxyethylpiparazine-N-4-butanesulfonic acid (HEPBS);
N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (HEPES):
N-2-hydroxyethylpiperazine-N-2-propanesulfonic acid (HEPPSO);
2-N-morpholinoethanesulfonic acid (MES);
9-N-morpholinobutanesulfonic acid (MOBS);
3-N-morpholinopropanesulfonic acid (MOPS);
3-N-morpholino-2-hydroxypropanesulfonic acid (MOPSO);
piperazine-N-N-bis-2-ethanesulfonic acid (PIPES);
piperazine-N-N-bis-2-hydroxypropanesulfonic acid (POPSO) ;
N-trishydroxymethyl-methyl-9-aminobutanesulfonic acid (TABS);
N-trishydroxymethyl-methyl-3-aminopropanesulfonic acid (TAPS);
3-N-trishydroxymethyl-methylamino-2-hydroxypropanesulfonic acid (TAPSO);
N-trishydroxymethyl-methyl-2-aminoethanesulfonic acid (TES);
N-trishydroxymethylmethylglycine (TRICINE):
trishydroxymethylaminomethane (Tris); or a polyhydroxylated amine.
2. The method according to claim 1, wherein the ionisabhe group is provided by the chemical species Tris, Bis-Tris, Bis-Tris Propane, Tricine or Bicine.
3. The method according to claim 1 or claim 2, wherein a plurality of the chemical species providing the ionisable groups are separately immobilised on a solid support by covalent or ionic bonding or by adsorption.
4. The method according to any one of claims 1 to 3, wherein the plurality of ionisable groups are separately attached to a polymer, said polymer being immobilised on a solid support by covalent or ionic bonding or by adsorption.
5. The method according to any one of claims 1 to 3, wherein the ionisable groups are polymerised, optionally by means of cross-linking reagents.
6. The method according to claim 5, wherein the polymer is immobilised on a solid support by covalent or ionic bonding or by adsorption.
7. The method according to claim 5, wherein the polymer is a solid.
8. The method according to any one of the preceding claims, wherein the ionisable groups are provided by a dimer or oligomer of Bis-Tris.
9. The method according to any one of the preceding claims, wherein the solid phase product comprises a plurality of Bis-Tris molecules attached to a polyacrylic acid backbone.
10. The method according to any one of the preceding claims, wherein the solid phase is selected from the group consisting of beads, particles, tubes, wells, probes, dipsticks, pipette tips, slides, fibers, membranes. papers, glass and plastics.
11. The method according to any one of the preceding claims, wherein the solid phase is magnetic beads or paramagnetic beads.
12. The method according to any one of the preceding claims, wherein the mild conditions are conditions at which said nucleic acid is not denatured and/or not degraded and/or not depurinated and/or substantially physiological conditions.
13. The method according to any one of the preceding claims, wherein the releasing step occurs at a pH of no more than about 10.5, preferably no more than about 9Ø
14. The method according to any one of the preceding claims, wherein the releasing step occurs at an ionic strength of no more than about 500mM, preferably no more than about 100mM.
15. The method according to anyone of the preceding claims, wherein the releasing step occurs at a temperature of no more than about 70°C, preferably no more than about 50°C.
16. The method according to claim 15, wherein the releasing step occurs at about room temperature.
17. The method according to any one of the preceding claims, wherein the releasing step comprises eluting the nucleic acid with a buffer solution suitable for the storage or further processing of the released nucleic acid.
18. The method according to any one of the preceding claims, wherein the buffer solution is a buffer solution suitable for PCR.
19. The method according to any one of the preceding claims, wherein the pKa of said ionisable group is between about 3.0 and 9.0, preferably between about 9.0 and 9Ø
20. The method according to claim 19, wherein the material comprises a positively ionisable group, the pKa of which is between about 5.0 and 8.0, preferably between about 6.0 and 7Ø
21. The method according to claim 20, wherein the material, comprises a weak base.
22. The method according to claim 20, wherein the material comprises a positively ionisable nitrogen. atom and one or more electronegative groups capable of lowering the pKa of the positively ionisable nitrogen atom.
23. The method according to any one of the preceding claims, wherein the method comprises:
(a) extracting nucleic acid from impurities in the sample by bringing the sample into contact with a material which comprises an ionisable group, wherein the material has a positive charge at a first pH, such that nucleic acid is bound to the. material;
(b) releasing the nucleic acid at a second, higher pH at which the charge on the material is negative, neutral or less positive:
(c) amplifying target nucleic acid in the sample in a PCR reaction;
(d) following the PCR reaction, adjusting sample to a lower pH to cause the amplified nucleic acid to bind to.
the material.
24. The method according to claim 23, further comprising the initial step of lysing a sample of cells to release nucleic acid and provide the sample treated in step (a).
25. The method according to claim 23 or claim 24, further comprising washing the material after step (a) to remove impurities present in the sample.
26. The method according to claim 25, wherein the washing step is carried out at a pH lower than the second pH so that the nucleic acid is substantially not released from the material.
27. The method according to any one of claims 23 to 26, wherein the releasing step (b) is carried out by adjusting the pH of the sample so that it is compatible with PCR or by adding PCR buffer.
28. The method according to claim 27, wherein the PCR
buffer is 10mM Tris.HCl, 50mM KCl at pH 8.5.
29. The method according to any one of claims 23 to 28, wherein in step (d) the amplified nucleic acid is isolated from buffer constituents and primers.
30. The method according to any one of claims 23 to 29, further comprising storing the nucleic acid sample after steps (a) or (d) and/or subjecting it to further manipulation.
31. The method according to any one of claims 23 to 30, further comprising releasing the nucleic acid sample from the material after step (d) and/or subjecting it to further manipulation.
32. The method according to any one of claims 23 to 31, wherein the material comprises a PCR tube or beads.
33. The method according to claim 32, wherein the beads are magnetic beads or paramagnetic beads.
34. The method according to any one of the preceding claims, wherein the material comprises an ionisable group having a pKa value, said pKa value being between the first and second pH, or within about 1.0 pH unit, preferably within about 0.5 pH unit, below said first pH.
35. The method according to claim 34, wherein said second pH is within about 3 pH units, preferably within about 2 pH units, of the pKa value.
36. the method according to any one of the preceding claims, wherein the method is for separating single stranded nucleic acid from double stranded nucleic acid.
37. The method according to any one of the preceding claims, wherein the method is for extracting single stranded nucleic acid, said method comprising a prior step of converting double stranded nucleic acid into single stranded nucleic acid.
38. The method according to any one of the preceding claims, wherein the material is a solid phase material.
39. The method according to any one of the preceding claims, wherein the binding step occurs in a solution having a concentration of 1M or less.
40. A solid phase product for use in a method in which the solid phase reversibly binds nucleic acid present in a sample, the product comprising a plurality of positively ionisable groups, wherein the ionisable groups are immobilised on a solid support and are capable at a first pH at which the ionisable groups are positively charged of binding nucleic acid present in a sample and are capable of releasing the nucleic acid at a second, higher, pH at which the charge on the ionisable groups i.s negative, neutral or less positive, the ionisable groups being provided by a chemical species which is selected from the group consisting of:
N-2-acetamido-2-aminoethanesulfonic acid (ACES);
N-2-acetamido-2-iminodiacetic acid (ADA);
amino methyl propanediol (AMP);
3-1,1-dimethyl-2-hydroxyethylamino-2-hydroxy propanesulfonic acid (AMPSO);
N,N-bis2-hydroxyethyl-2-aminoethanesulfonic acid (BES);
N,N-bis-2-hydroxyethylglycine (BICINE);
bis-2-hydroxyethyliminotrishydroxymethylmethane (Bis-Tris);
1,3-bistrishydroxymethylmethylaminopropane (Bis-Tris Propane);
4-cyclohexylamino-1-butane sulfonic acid (CABS);
3-cyclohexylamino-1-propane sulfonic acid (CAPS);
3-cyclohexylamino-2-hydroxy-1-propane sulfonic acid (CAPSO);
2-N-cyclohexylaminoethanesulfonic acid (CHES);
3-N,N-bis-2-hydroxyethylamino-2-hydroxypropanesulfonic acid (DIPSO):
N-2-hydroxyethylpiperazine-N-3-propanesulfonic acid (EPPS);
N-2-hydroxyethylpiperazine-N-4-butanesulfonic acid (HEPBS):
N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (HEPES);
N-2-hydroxyethylpiperazine-N-2-propanesulfonic acid (HEPPSO);
2-N-morpholinoethanesulfonic acid (MES);
4-N-morpholinobutanesulfonic acid (MOBS):
3-N-morpholinopropanesulfonic acid (MOPS);
3-N-morpholino-2-hydroxypropanesulfonic acid (MOPSO);
piperazine-N-N-bis-2-ethanesulfonic acid (PIPES);
piperazine-N-N-bis-2-hydroxypropanesulfonic acid (POPSO);
N-trishydroxymethyl-methyl-4-aminobutanesulfonic acid (TABS);
N-trishydroxymethyl-methyl-3-aminopropanesulfonic acid (TAPS);
3-N-trishydroxymethyl-methylamino-2-hydroxypropanesulfonic acid (TAPSO);
N-trishydroxymethyl-methyl-2-aminoethanesulfonic acid (TES);
N-trishydroxymethylmethylglycine (TRICINE);
trishydroxymethylaminomethane (Tris); or a polyhydroxylated amine.
41. The product according to claim 40, wherein the ionisable group is provided by the chemical species Tris, Bis-Tris, Bis-Tris Propane, Tricine or Bicine.
42. The product according to claim 40 or claim 41, wherein the plurality of ionisable groups are separately immobilised on a solid support by covalent or ionic bonding or by adsorption.
43. The product according to claim 40 or claim 41, wherein the plurality of ionisable groups are separately attached to a polymer, said polymer being immobilised on a solid support by covalent or ionic bonding or by adsorption.
44. The product according to claim 40 or claim 41, wherein the ionisable groups are polymerised, optionally by means of cross-linking reagents.
45. The product according to claim 44, wherein the polymer is immobilised on a solid support by covalent or ionic bonding or by adsorption.
46. The product according to claim 44, wherein the polymer is a solid.
47. The product according to any one of claims 40 to 46, wherein the ionisable groups are provided by a dimer or oligomer of Bis-Tris.
98. The product according to any one of claims 40 to 96, wherein the solid phase product comprises a plurality of Bis-Tris molecules attached to a polyacrylic acid Backbone.
49. The product according to any one of claims 40 to 48, wherein the solid phase is selected from the group consisting of beads, particles, tubes, wells, probes, dipsticks, pipette tips, slides, fibers, membranes, papers, glass and plastics.
50. The product according to claim 99, wherein the solid phase is magnetic beads or paramagnetic beads.
1. A method for extracting nucleic acid from a sample containing nucleic acid, which method comprises:
at a first pH, bringing the sample into contact with a material which comprises an ionisable group immobilised on a solid support, wherein the material has a positive charge at said first pH, such that nucleic acid is bound to the material: and releasing the nucleic acid at a second, higher, pH
at which the charge on the material is negative, neutral or less positive, wherein the release of the nucleic acid occurs under mild conditions and the ionisable group is provided by a chemical species selected from:
N-2-acetamido-2-aminoethanesulfonic acid (ACES);
N-2-acetamido-2-iminodiacetic acid (ADA):
amino methyl propanediol (AMP);
3-1,1-dimethyl-2-hydroxyethylamino-2-hydroxy propanesulfonic acid (AMPSO):
N,N-bis2-hydroxyethyl-2-aminoethanesulfonic acid (BES):
N,N-bis-2-hydroxyethylglycine (BICINE);
bis-2-hydroxyethyliminotrishydroxymethylmethane (Bis-Tris);
1,3-bistrishydroxymethylmethylaminopropane (Bis-Tris Propane):
4-cyclohexylamino-1-butane sulfonic acid (CABS);
3-cyclohexylamino-1-propane sulfonic acid (CAPS);
3-cyclohexylamino-2-hydroxy-1-propane sulfonic acid (CAPSO);
2-N-cyclohexylaminoethanesulfonic acid (CHES);
3-N,N-bis-2-hydroxyethylamino-2-hydroxypropanesulfonic acid (D.tau.PSO);
N-2-hydroxyethylpiperazine-N-3-propanesulfonic acid (EPPS);
N-2-hydroxyethylpiparazine-N-4-butanesulfonic acid (HEPBS);
N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (HEPES):
N-2-hydroxyethylpiperazine-N-2-propanesulfonic acid (HEPPSO);
2-N-morpholinoethanesulfonic acid (MES);
9-N-morpholinobutanesulfonic acid (MOBS);
3-N-morpholinopropanesulfonic acid (MOPS);
3-N-morpholino-2-hydroxypropanesulfonic acid (MOPSO);
piperazine-N-N-bis-2-ethanesulfonic acid (PIPES);
piperazine-N-N-bis-2-hydroxypropanesulfonic acid (POPSO) ;
N-trishydroxymethyl-methyl-9-aminobutanesulfonic acid (TABS);
N-trishydroxymethyl-methyl-3-aminopropanesulfonic acid (TAPS);
3-N-trishydroxymethyl-methylamino-2-hydroxypropanesulfonic acid (TAPSO);
N-trishydroxymethyl-methyl-2-aminoethanesulfonic acid (TES);
N-trishydroxymethylmethylglycine (TRICINE):
trishydroxymethylaminomethane (Tris); or a polyhydroxylated amine.
2. The method according to claim 1, wherein the ionisabhe group is provided by the chemical species Tris, Bis-Tris, Bis-Tris Propane, Tricine or Bicine.
3. The method according to claim 1 or claim 2, wherein a plurality of the chemical species providing the ionisable groups are separately immobilised on a solid support by covalent or ionic bonding or by adsorption.
4. The method according to any one of claims 1 to 3, wherein the plurality of ionisable groups are separately attached to a polymer, said polymer being immobilised on a solid support by covalent or ionic bonding or by adsorption.
5. The method according to any one of claims 1 to 3, wherein the ionisable groups are polymerised, optionally by means of cross-linking reagents.
6. The method according to claim 5, wherein the polymer is immobilised on a solid support by covalent or ionic bonding or by adsorption.
7. The method according to claim 5, wherein the polymer is a solid.
8. The method according to any one of the preceding claims, wherein the ionisable groups are provided by a dimer or oligomer of Bis-Tris.
9. The method according to any one of the preceding claims, wherein the solid phase product comprises a plurality of Bis-Tris molecules attached to a polyacrylic acid backbone.
10. The method according to any one of the preceding claims, wherein the solid phase is selected from the group consisting of beads, particles, tubes, wells, probes, dipsticks, pipette tips, slides, fibers, membranes. papers, glass and plastics.
11. The method according to any one of the preceding claims, wherein the solid phase is magnetic beads or paramagnetic beads.
12. The method according to any one of the preceding claims, wherein the mild conditions are conditions at which said nucleic acid is not denatured and/or not degraded and/or not depurinated and/or substantially physiological conditions.
13. The method according to any one of the preceding claims, wherein the releasing step occurs at a pH of no more than about 10.5, preferably no more than about 9Ø
14. The method according to any one of the preceding claims, wherein the releasing step occurs at an ionic strength of no more than about 500mM, preferably no more than about 100mM.
15. The method according to anyone of the preceding claims, wherein the releasing step occurs at a temperature of no more than about 70°C, preferably no more than about 50°C.
16. The method according to claim 15, wherein the releasing step occurs at about room temperature.
17. The method according to any one of the preceding claims, wherein the releasing step comprises eluting the nucleic acid with a buffer solution suitable for the storage or further processing of the released nucleic acid.
18. The method according to any one of the preceding claims, wherein the buffer solution is a buffer solution suitable for PCR.
19. The method according to any one of the preceding claims, wherein the pKa of said ionisable group is between about 3.0 and 9.0, preferably between about 9.0 and 9Ø
20. The method according to claim 19, wherein the material comprises a positively ionisable group, the pKa of which is between about 5.0 and 8.0, preferably between about 6.0 and 7Ø
21. The method according to claim 20, wherein the material, comprises a weak base.
22. The method according to claim 20, wherein the material comprises a positively ionisable nitrogen. atom and one or more electronegative groups capable of lowering the pKa of the positively ionisable nitrogen atom.
23. The method according to any one of the preceding claims, wherein the method comprises:
(a) extracting nucleic acid from impurities in the sample by bringing the sample into contact with a material which comprises an ionisable group, wherein the material has a positive charge at a first pH, such that nucleic acid is bound to the. material;
(b) releasing the nucleic acid at a second, higher pH at which the charge on the material is negative, neutral or less positive:
(c) amplifying target nucleic acid in the sample in a PCR reaction;
(d) following the PCR reaction, adjusting sample to a lower pH to cause the amplified nucleic acid to bind to.
the material.
24. The method according to claim 23, further comprising the initial step of lysing a sample of cells to release nucleic acid and provide the sample treated in step (a).
25. The method according to claim 23 or claim 24, further comprising washing the material after step (a) to remove impurities present in the sample.
26. The method according to claim 25, wherein the washing step is carried out at a pH lower than the second pH so that the nucleic acid is substantially not released from the material.
27. The method according to any one of claims 23 to 26, wherein the releasing step (b) is carried out by adjusting the pH of the sample so that it is compatible with PCR or by adding PCR buffer.
28. The method according to claim 27, wherein the PCR
buffer is 10mM Tris.HCl, 50mM KCl at pH 8.5.
29. The method according to any one of claims 23 to 28, wherein in step (d) the amplified nucleic acid is isolated from buffer constituents and primers.
30. The method according to any one of claims 23 to 29, further comprising storing the nucleic acid sample after steps (a) or (d) and/or subjecting it to further manipulation.
31. The method according to any one of claims 23 to 30, further comprising releasing the nucleic acid sample from the material after step (d) and/or subjecting it to further manipulation.
32. The method according to any one of claims 23 to 31, wherein the material comprises a PCR tube or beads.
33. The method according to claim 32, wherein the beads are magnetic beads or paramagnetic beads.
34. The method according to any one of the preceding claims, wherein the material comprises an ionisable group having a pKa value, said pKa value being between the first and second pH, or within about 1.0 pH unit, preferably within about 0.5 pH unit, below said first pH.
35. The method according to claim 34, wherein said second pH is within about 3 pH units, preferably within about 2 pH units, of the pKa value.
36. the method according to any one of the preceding claims, wherein the method is for separating single stranded nucleic acid from double stranded nucleic acid.
37. The method according to any one of the preceding claims, wherein the method is for extracting single stranded nucleic acid, said method comprising a prior step of converting double stranded nucleic acid into single stranded nucleic acid.
38. The method according to any one of the preceding claims, wherein the material is a solid phase material.
39. The method according to any one of the preceding claims, wherein the binding step occurs in a solution having a concentration of 1M or less.
40. A solid phase product for use in a method in which the solid phase reversibly binds nucleic acid present in a sample, the product comprising a plurality of positively ionisable groups, wherein the ionisable groups are immobilised on a solid support and are capable at a first pH at which the ionisable groups are positively charged of binding nucleic acid present in a sample and are capable of releasing the nucleic acid at a second, higher, pH at which the charge on the ionisable groups i.s negative, neutral or less positive, the ionisable groups being provided by a chemical species which is selected from the group consisting of:
N-2-acetamido-2-aminoethanesulfonic acid (ACES);
N-2-acetamido-2-iminodiacetic acid (ADA);
amino methyl propanediol (AMP);
3-1,1-dimethyl-2-hydroxyethylamino-2-hydroxy propanesulfonic acid (AMPSO);
N,N-bis2-hydroxyethyl-2-aminoethanesulfonic acid (BES);
N,N-bis-2-hydroxyethylglycine (BICINE);
bis-2-hydroxyethyliminotrishydroxymethylmethane (Bis-Tris);
1,3-bistrishydroxymethylmethylaminopropane (Bis-Tris Propane);
4-cyclohexylamino-1-butane sulfonic acid (CABS);
3-cyclohexylamino-1-propane sulfonic acid (CAPS);
3-cyclohexylamino-2-hydroxy-1-propane sulfonic acid (CAPSO);
2-N-cyclohexylaminoethanesulfonic acid (CHES);
3-N,N-bis-2-hydroxyethylamino-2-hydroxypropanesulfonic acid (DIPSO):
N-2-hydroxyethylpiperazine-N-3-propanesulfonic acid (EPPS);
N-2-hydroxyethylpiperazine-N-4-butanesulfonic acid (HEPBS):
N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (HEPES);
N-2-hydroxyethylpiperazine-N-2-propanesulfonic acid (HEPPSO);
2-N-morpholinoethanesulfonic acid (MES);
4-N-morpholinobutanesulfonic acid (MOBS):
3-N-morpholinopropanesulfonic acid (MOPS);
3-N-morpholino-2-hydroxypropanesulfonic acid (MOPSO);
piperazine-N-N-bis-2-ethanesulfonic acid (PIPES);
piperazine-N-N-bis-2-hydroxypropanesulfonic acid (POPSO);
N-trishydroxymethyl-methyl-4-aminobutanesulfonic acid (TABS);
N-trishydroxymethyl-methyl-3-aminopropanesulfonic acid (TAPS);
3-N-trishydroxymethyl-methylamino-2-hydroxypropanesulfonic acid (TAPSO);
N-trishydroxymethyl-methyl-2-aminoethanesulfonic acid (TES);
N-trishydroxymethylmethylglycine (TRICINE);
trishydroxymethylaminomethane (Tris); or a polyhydroxylated amine.
41. The product according to claim 40, wherein the ionisable group is provided by the chemical species Tris, Bis-Tris, Bis-Tris Propane, Tricine or Bicine.
42. The product according to claim 40 or claim 41, wherein the plurality of ionisable groups are separately immobilised on a solid support by covalent or ionic bonding or by adsorption.
43. The product according to claim 40 or claim 41, wherein the plurality of ionisable groups are separately attached to a polymer, said polymer being immobilised on a solid support by covalent or ionic bonding or by adsorption.
44. The product according to claim 40 or claim 41, wherein the ionisable groups are polymerised, optionally by means of cross-linking reagents.
45. The product according to claim 44, wherein the polymer is immobilised on a solid support by covalent or ionic bonding or by adsorption.
46. The product according to claim 44, wherein the polymer is a solid.
47. The product according to any one of claims 40 to 46, wherein the ionisable groups are provided by a dimer or oligomer of Bis-Tris.
98. The product according to any one of claims 40 to 96, wherein the solid phase product comprises a plurality of Bis-Tris molecules attached to a polyacrylic acid Backbone.
49. The product according to any one of claims 40 to 48, wherein the solid phase is selected from the group consisting of beads, particles, tubes, wells, probes, dipsticks, pipette tips, slides, fibers, membranes, papers, glass and plastics.
50. The product according to claim 99, wherein the solid phase is magnetic beads or paramagnetic beads.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/736,632 US6914137B2 (en) | 1997-12-06 | 2000-12-14 | Isolation of nucleic acids |
US09/736,632 | 2000-12-14 | ||
PCT/GB2001/005524 WO2002048164A2 (en) | 2000-12-14 | 2001-12-14 | Isolation of nucleic acids |
Publications (1)
Publication Number | Publication Date |
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CA2432075A1 true CA2432075A1 (en) | 2002-06-20 |
Family
ID=24960633
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CA002432075A Abandoned CA2432075A1 (en) | 2000-12-14 | 2001-12-14 | Isolation of nucleic acids |
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US (8) | US6914137B2 (en) |
EP (2) | EP1345952B1 (en) |
JP (1) | JP4868697B2 (en) |
AT (1) | ATE283275T1 (en) |
AU (1) | AU2002216203A1 (en) |
CA (1) | CA2432075A1 (en) |
DE (1) | DE60107468T2 (en) |
DK (1) | DK1345952T3 (en) |
ES (1) | ES2233560T3 (en) |
WO (1) | WO2002048164A2 (en) |
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-
2000
- 2000-12-14 US US09/736,632 patent/US6914137B2/en not_active Expired - Lifetime
-
2001
- 2001-12-14 EP EP01270542A patent/EP1345952B1/en not_active Expired - Lifetime
- 2001-12-14 AU AU2002216203A patent/AU2002216203A1/en not_active Abandoned
- 2001-12-14 ES ES01270542T patent/ES2233560T3/en not_active Expired - Lifetime
- 2001-12-14 JP JP2002549695A patent/JP4868697B2/en not_active Expired - Lifetime
- 2001-12-14 CA CA002432075A patent/CA2432075A1/en not_active Abandoned
- 2001-12-14 WO PCT/GB2001/005524 patent/WO2002048164A2/en active IP Right Grant
- 2001-12-14 DK DK01270542T patent/DK1345952T3/en active
- 2001-12-14 EP EP04017291A patent/EP1473299A3/en not_active Withdrawn
- 2001-12-14 DE DE60107468T patent/DE60107468T2/en not_active Expired - Lifetime
- 2001-12-14 AT AT01270542T patent/ATE283275T1/en not_active IP Right Cessation
-
2002
- 2002-08-29 US US10/232,135 patent/US20030008320A1/en not_active Abandoned
- 2002-08-30 US US10/232,971 patent/US20030054395A1/en not_active Abandoned
-
2007
- 2007-06-12 US US11/761,956 patent/US20070231892A1/en not_active Abandoned
-
2008
- 2008-06-11 US US12/137,125 patent/US20080305528A1/en not_active Abandoned
-
2012
- 2012-01-30 US US13/361,815 patent/US20120196944A1/en not_active Abandoned
- 2012-04-13 US US13/447,130 patent/US20120197009A1/en not_active Abandoned
-
2013
- 2013-05-20 US US13/898,400 patent/US20130338245A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
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US20070231892A1 (en) | 2007-10-04 |
EP1345952A2 (en) | 2003-09-24 |
JP4868697B2 (en) | 2012-02-01 |
US20120197009A1 (en) | 2012-08-02 |
WO2002048164A3 (en) | 2002-10-17 |
US20030054395A1 (en) | 2003-03-20 |
JP2004521881A (en) | 2004-07-22 |
US20010018513A1 (en) | 2001-08-30 |
US20030008320A1 (en) | 2003-01-09 |
EP1473299A2 (en) | 2004-11-03 |
EP1345952B1 (en) | 2004-11-24 |
DE60107468T2 (en) | 2005-12-29 |
DE60107468D1 (en) | 2004-12-30 |
WO2002048164A2 (en) | 2002-06-20 |
US20120196944A1 (en) | 2012-08-02 |
US6914137B2 (en) | 2005-07-05 |
ATE283275T1 (en) | 2004-12-15 |
DK1345952T3 (en) | 2005-03-21 |
EP1473299A3 (en) | 2006-08-16 |
ES2233560T3 (en) | 2005-06-16 |
AU2002216203A1 (en) | 2002-06-24 |
US20080305528A1 (en) | 2008-12-11 |
US20130338245A1 (en) | 2013-12-19 |
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