CA2445127A1 - Electro-optical sensing device with reference channel - Google Patents
Electro-optical sensing device with reference channel Download PDFInfo
- Publication number
- CA2445127A1 CA2445127A1 CA002445127A CA2445127A CA2445127A1 CA 2445127 A1 CA2445127 A1 CA 2445127A1 CA 002445127 A CA002445127 A CA 002445127A CA 2445127 A CA2445127 A CA 2445127A CA 2445127 A1 CA2445127 A1 CA 2445127A1
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- Prior art keywords
- analyte
- sensing device
- indicator
- indicator element
- elements
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6432—Quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7769—Measurement method of reaction-produced change in sensor
- G01N2021/7786—Fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7793—Sensor comprising plural indicators
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7796—Special mountings, packaging of indicators
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
- G01N21/783—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour for analysing gases
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
- Y10T436/144444—Glucose
Abstract
An electro-optical sensing device for detecting the presence and concentrati on of an analyte in a liquid or gaseous medium includes a pair of indicator elements positioned to receive radiation from a radiation source and transmi t radiation to a pair of photosensitive elements. The indicator elements each contain indicator molecules having an optical characteristic responsive to t he presence of an analyte; however, one of the indicator elements is covered by an analyte-impermeable chamber that renders the indicator element insensitiv e to the presence of the analyte in the medium outside the chamber so that it can be used as a reference to cancel environmental and systemic variables th at affect both indicator elements. The chamber preferably holds an analyte- containing fluid in contact with the reference indicator element so that the indicator elements operate under nominally identical conditions. The indicat or element used to measure the analyte in the external medium is preferably als o covered, but in a manner that provides direct contact between the analyte an d the indicator element.
Claims (40)
1. An electro-optical sensing device for detecting the presence of an analyte in a medium, said sensing device comprising:
a. a radiation source that emits radiation;
b. a first and a second photosensitive elements configured to receive radiation and output an electrical signal in response thereto;
c. a first and a second indicator elements positioned to receive radiation from the radiation source and to transmit radiation to said first and second photosensitive elements, said first and second indicator elements each containing indicator molecules with an optical characteristic responsive to the presence of an analyte; and wherein said first indicator element is exposed to an exterior of the device and is responsive to a presence of an analyte in a medium external to the device, and wherein said second indicator element is at least partially covered and is insensitive to the presence of the analyte in the medium external to the device.
a. a radiation source that emits radiation;
b. a first and a second photosensitive elements configured to receive radiation and output an electrical signal in response thereto;
c. a first and a second indicator elements positioned to receive radiation from the radiation source and to transmit radiation to said first and second photosensitive elements, said first and second indicator elements each containing indicator molecules with an optical characteristic responsive to the presence of an analyte; and wherein said first indicator element is exposed to an exterior of the device and is responsive to a presence of an analyte in a medium external to the device, and wherein said second indicator element is at least partially covered and is insensitive to the presence of the analyte in the medium external to the device.
2. The sensing device of claim 1, comprising an analyte-impermeable chamber covering said second indicator element so as to render said second indicator element insensitive to the presence of the analyte in the medium external to the device.
3. The sensing device of claim 2, wherein said analyte-impermeable chamber is a hermetically sealed chamber.
4. The sensing device of claim 2, wherein said chamber contains a fluid in contact with said second indicator element.
5. The sensing device of claim 4, wherein said fluid is an analyte-containing fluid in contact with said second indicator element.
6. The sensing device of claim 5, wherein said analyte-containing fluid is a liquid.
7. The sensing device of claim 5, wherein said analyte-containing fluid is a gas.
8. The sensing device of claim 5, wherein an analyte in said analyte-containing fluid is the same type of analyte to be detected in the external medium.
9. The sensing device of claim 5, wherein an analyte in said analyte-containing fluid is a different type of analyte than the analyte to be detected in the external medium.
10. The sensing device of claim 1, wherein said indicator molecule has an optical characteristic responsive to the presence of oxygen.
11. The sensing device of claim 10, wherein said indicator molecule is tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) perchlorate.
12. The sensing device of claim 2, further comprising a housing, wherein said first and second indicator elements, said first and second photosensitive elements, and said radiation source are mounted in said housing.
13. The sensing device of claim 12, further comprising an analyte-impermeable cover mounted on the housing which defines at least a portion of the chamber covering the second indicator element.
14. The sensing device of claim 13, wherein said housing has a first opening, and further wherein said analyte-impermeable cover and said second indicator element are mounted on opposite sides of said first opening to define the chamber therebetween.
15. The sensing device of claim 14, wherein said analyte impermeable cover comprises a plug having a flange extending outward from the top of the plug, said flange defining a contact surface for contacting an upper surface of the housing around said second opening and forming a hermetic seal to prevent migration of analyte containing fluids from the external meduim into the chamber.
16. The sensing device of claim 13, wherein said analyte-impermeable cover is formed of a metal.
17. The sensing device of claim 13, further comprising an analyte-permeable cover mounted on the housing over the first indicator element.
18. The sensing device of claim 17, wherein said analyte-permeable cover includes a bore formed therethrough to provide fluid communication between the first indicator element and the external medium.
19. The sensing device of claim 17, wherein said housing has a second opening, and further wherein said analyte-permeable cover and said first indicator element are mounted on opposite sides of said second opening.
20. The sensing device of claim 18, wherein said analyte permeable cover and said analyte-impermeable cover are substantially similar in configuration with the exception of the bore.
21. The sensing device of claim 1, wherein said first indicator element and said first photosensitive comprise a signal channel, and wherein said second indicator element and said second photosensitive element comprise a reference channel.
22. The sensing device of claim 21, wherein said signal channel and said reference channel are incorporated into a chip-like structure with leads extending therefrom.
23. The sensing device of claim 12, wherein said housing comprises a top wall, side walls depending from said top wall, said side walls having bottom edges which are mounted on a top surface of a substrate so as to maintain said top wall in vertically spaced relation to said substrate.
24. The sensing device of claim 23, wherein a region between said housing and said substrate contains a waveguide material.
25. The sensing device of claim 1, wherein said radiation source is a light emitting diode.
26. The sensing device of claim 13, wherein said analyte-impermeable cover is formed of a material having good thermal conductivity so that an interior of the chamber will experience temperature changes similar to temperature changes in the external medium.
27. The sensing device of claim 13, wherein said analyte-impermeable cover is formed of a transparent material.
28. The sensing device of claim 7, wherein said gas is air.
29. The sensing device of claim 21, wherein power to the sensing device is drawn from an external source through said leads.
30. The sensing device of claim 1, wherein power to the sensing device is provided from an internal power source.
31. The sensing device of claim 30, wherein said internal power source is a battery.
32. The sensing device of claim 1, wherein power to the sensing device is provided from an external source through an induction circuit formed in the sensing device.
33. The sensing device of claim 1, wherein said indicator molecule has an optical characteristic responsive to the presence of glucose.
34. An electro-optical sensing device for detecting the presence of an analyte in a medium, said sensing device comprising:
a. a radiation source that emits radiation;
b. a first and a second photosensitive elements configured to receive radiation and output an electrical signal in response thereto;
c. a first and a second indicator elements positioned to receive radiation from the radiation source and to transmit radiation to said first and second photosensitive elements, said first and second indicator elements each containing indicator molecules with an optical characteristic responsive to the presence of an analyte; and d. a hermetically sealed chamber covering said second indicator element so as to render said second indicator element insensitive to the presence of the analyte in a medium external to the device, wherein said chamber contains an analyte-containing fluid in contact with said second indicator element.
a. a radiation source that emits radiation;
b. a first and a second photosensitive elements configured to receive radiation and output an electrical signal in response thereto;
c. a first and a second indicator elements positioned to receive radiation from the radiation source and to transmit radiation to said first and second photosensitive elements, said first and second indicator elements each containing indicator molecules with an optical characteristic responsive to the presence of an analyte; and d. a hermetically sealed chamber covering said second indicator element so as to render said second indicator element insensitive to the presence of the analyte in a medium external to the device, wherein said chamber contains an analyte-containing fluid in contact with said second indicator element.
35. The sensing device of claim 34, further comprising a housing, wherein said first and second indicator elements, said first and second photosensitive elements, and said radiation source are mounted in said housing.
36. The sensing device of claim 34, further comprising an analyte-impermeable cover mounted on the housing which defines at least a portion of the chamber covering the second indicator element.
37. The sensing device of claim 34, further comprising an analyte-permeable cover mounted on the housing over the first indicator element.
38. The sensing device of claim 37, wherein said analyte-permeable cover includes a bore formed therethrough to provide fluid communication between the first indicator element and the external medium.
39. The sensing device of claim 34, wherein said first indicator element and said first photosensitive comprise a signal channel, and wherein said second indicator element and said second photosensitive element comprise a reference channel.
40. A method for detecting an analyte of interest in a medium, said method comprising:
a. providing an electro-optical sensing device containing a first and a second indicator elements each containing indicator molecules having an optical characteristic responsive to the presence of an analyte and positioned to receive excitation radiation from a radiation source and to transmit resultant radiation to a pair of photosensitive elements, and wherein said second indicator elements is covered by an analyte-impermeable chamber that renders the indicator element insensitive to the presence of the analyte in the medium external to the device;
b. introducing a medium to contact said first indicator element;
activating said radiation source to emit radiation to said first and second indicator elements, d. optically detecting an optical response of said first and second indicator elements; and e. evaluating said response to determine the presence or concentration of at least one analyte of interest in the sample, wherein said response detected from said second indicator element is used as a reference to cancel variables that affect both said first and second indicator elements.
a. providing an electro-optical sensing device containing a first and a second indicator elements each containing indicator molecules having an optical characteristic responsive to the presence of an analyte and positioned to receive excitation radiation from a radiation source and to transmit resultant radiation to a pair of photosensitive elements, and wherein said second indicator elements is covered by an analyte-impermeable chamber that renders the indicator element insensitive to the presence of the analyte in the medium external to the device;
b. introducing a medium to contact said first indicator element;
activating said radiation source to emit radiation to said first and second indicator elements, d. optically detecting an optical response of said first and second indicator elements; and e. evaluating said response to determine the presence or concentration of at least one analyte of interest in the sample, wherein said response detected from said second indicator element is used as a reference to cancel variables that affect both said first and second indicator elements.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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US28849801P | 2001-05-04 | 2001-05-04 | |
US60/288,498 | 2001-05-04 | ||
PCT/US2002/013734 WO2002090951A1 (en) | 2001-05-04 | 2002-05-03 | Electro-optical sensing device with reference channel |
Publications (2)
Publication Number | Publication Date |
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CA2445127A1 true CA2445127A1 (en) | 2002-11-14 |
CA2445127C CA2445127C (en) | 2011-12-13 |
Family
ID=23107373
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2445127A Expired - Fee Related CA2445127C (en) | 2001-05-04 | 2002-05-03 | Electro-optical sensing device with reference channel |
Country Status (10)
Country | Link |
---|---|
US (3) | US7135342B2 (en) |
EP (1) | EP1386140A1 (en) |
JP (1) | JP2004529352A (en) |
CN (1) | CN100520372C (en) |
AU (1) | AU2002256413B2 (en) |
BR (1) | BR0209357A (en) |
CA (1) | CA2445127C (en) |
MX (1) | MXPA03010064A (en) |
TW (1) | TWI252311B (en) |
WO (1) | WO2002090951A1 (en) |
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-
2002
- 2002-05-03 AU AU2002256413A patent/AU2002256413B2/en not_active Ceased
- 2002-05-03 EP EP02725876A patent/EP1386140A1/en not_active Withdrawn
- 2002-05-03 JP JP2002588162A patent/JP2004529352A/en active Pending
- 2002-05-03 MX MXPA03010064A patent/MXPA03010064A/en active IP Right Grant
- 2002-05-03 CA CA2445127A patent/CA2445127C/en not_active Expired - Fee Related
- 2002-05-03 US US10/137,329 patent/US7135342B2/en not_active Expired - Lifetime
- 2002-05-03 TW TW091109245A patent/TWI252311B/en not_active IP Right Cessation
- 2002-05-03 WO PCT/US2002/013734 patent/WO2002090951A1/en active IP Right Grant
- 2002-05-03 BR BR0209357-0A patent/BR0209357A/en not_active Application Discontinuation
- 2002-05-03 CN CNB028093674A patent/CN100520372C/en not_active Expired - Fee Related
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2006
- 2006-11-13 US US11/595,986 patent/US20070059210A1/en not_active Abandoned
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2010
- 2010-11-23 US US12/953,067 patent/US20110064617A1/en not_active Abandoned
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MXPA03010064A (en) | 2004-03-09 |
CA2445127C (en) | 2011-12-13 |
CN1507560A (en) | 2004-06-23 |
JP2004529352A (en) | 2004-09-24 |
EP1386140A1 (en) | 2004-02-04 |
CN100520372C (en) | 2009-07-29 |
WO2002090951A1 (en) | 2002-11-14 |
TWI252311B (en) | 2006-04-01 |
AU2002256413B2 (en) | 2007-06-07 |
US20070059210A1 (en) | 2007-03-15 |
BR0209357A (en) | 2004-06-08 |
US7135342B2 (en) | 2006-11-14 |
US20020164813A1 (en) | 2002-11-07 |
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