CA2476286A1 - Method of recovering micromaterial and device - Google Patents

Method of recovering micromaterial and device Download PDF

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Publication number
CA2476286A1
CA2476286A1 CA002476286A CA2476286A CA2476286A1 CA 2476286 A1 CA2476286 A1 CA 2476286A1 CA 002476286 A CA002476286 A CA 002476286A CA 2476286 A CA2476286 A CA 2476286A CA 2476286 A1 CA2476286 A1 CA 2476286A1
Authority
CA
Canada
Prior art keywords
micromaterial
medium
micromaterials
sol
gel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002476286A
Other languages
French (fr)
Other versions
CA2476286C (en
Inventor
Fumihito Arai
Toshio Fukuda
Akihiko Ichikawa
Tohoru Katsuragi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japan Science and Technology Agency
Original Assignee
Japan Science And Technology Agency
Fumihito Arai
Toshio Fukuda
Akihiko Ichikawa
Tohoru Katsuragi
Japan Science And Technology Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Science And Technology Agency, Fumihito Arai, Toshio Fukuda, Akihiko Ichikawa, Tohoru Katsuragi, Japan Science And Technology Corporation filed Critical Japan Science And Technology Agency
Publication of CA2476286A1 publication Critical patent/CA2476286A1/en
Application granted granted Critical
Publication of CA2476286C publication Critical patent/CA2476286C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00495—Means for heating or cooling the reaction vessels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00639—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium
    • B01J2219/00644—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium the porous medium being present in discrete locations, e.g. gel pads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1861—Means for temperature control using radiation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1861—Means for temperature control using radiation
    • B01L2300/1872—Infrared light
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • G01N15/1433—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G01N2001/282—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on with mapping; Identification of areas; Spatial correlated pattern
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G01N2001/2833—Collecting samples on a sticky, tacky, adhesive surface
    • G01N2001/284—Collecting samples on a sticky, tacky, adhesive surface using local activation of adhesive, i.e. Laser Capture Microdissection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2873—Cutting or cleaving
    • G01N2001/2886—Laser cutting, e.g. tissue catapult
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1497—Particle shape

Abstract

A recovery method for specific micromaterials such as DNA
molecules, cells, microorganisms and the like, approximately several µm in size, from a mixture containing other micromaterials is presented.
A substance (for example, methylcellulose) that undergoes a reversible gel to sol phase transition is utilized, and a micromaterial is accurately and conveniently recovered. The present invention is a method of recovering a desired micromaterial in a system comprising a medium that undergoes a reversible phase transition between a sol and a gel, a support material and micromaterials, comprising the steps of converting the medium surrounding the micromaterial to be recovered into a gel locally to immobilize said micromaterial on said support along with the gelled medium, removing the medium and micromaterials not immobilized on said support material and converting the gelled medium to a sol to recover the micromaterial.
CA002476286A 2002-05-07 2003-01-14 Method of recovering micromaterial and device Expired - Fee Related CA2476286C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2002131656 2002-05-07
JP2002/131656 2002-05-07
PCT/JP2003/000231 WO2003095088A1 (en) 2002-05-07 2003-01-14 Method and apparatus for collecting micromaterial

Publications (2)

Publication Number Publication Date
CA2476286A1 true CA2476286A1 (en) 2003-11-20
CA2476286C CA2476286C (en) 2008-03-18

Family

ID=29416614

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002476286A Expired - Fee Related CA2476286C (en) 2002-05-07 2003-01-14 Method of recovering micromaterial and device

Country Status (5)

Country Link
US (1) US7824854B2 (en)
EP (1) EP1504812B1 (en)
JP (1) JP4228114B2 (en)
CA (1) CA2476286C (en)
WO (1) WO2003095088A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100883775B1 (en) * 2007-03-22 2009-02-18 명지대학교 산학협력단 Electrochemical Detector Integrated on Microfabricated Capilliary Electrophoresis Chip and Method of Manufacturing the Same
JP2009045002A (en) * 2007-08-20 2009-03-05 Oki Electric Ind Co Ltd Cell patterning apparatus and cell patterning method
ES2654321T3 (en) * 2007-09-27 2018-02-13 Samyang Biopharmaceuticals Corporation Reversible phase sol-gel hydrogel matrices and uses thereof
WO2009061392A1 (en) * 2007-11-05 2009-05-14 President And Fellows Of Harvard College Forming gel structures using microfluidic channels
US8628953B2 (en) 2007-11-29 2014-01-14 Hitachi Plant Technologies, Ltd. Capturing carrier, capturing device, analysis system using the same, and method for capturing and testing microorganisms
JP2010046012A (en) * 2008-08-21 2010-03-04 Konica Minolta Holdings Inc Substrate and method for cell culture
DE102013202054A1 (en) * 2012-02-10 2013-08-14 Arges Gmbh Contrasting method by laser and apparatus for performing a contrasting method
EP2855695A4 (en) * 2012-05-29 2016-01-13 Univ California Systems, methods and components for isolating cells from a fluid sample
ES2624959T3 (en) * 2012-12-21 2017-07-18 diamond invention UG (haftungsbeschränkt) Fluidic system with absorbent material and switchable polymer gel
IL299366A (en) * 2015-11-23 2023-02-01 Berkeley Lights Inc In situ-generated microfluidic isolation structures, kits and methods of use thereof
KR102466814B1 (en) * 2015-12-08 2022-11-11 버클리 라잇츠, 인크. Microfluidic devices and kits and methods for their use

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US4689102A (en) * 1985-01-25 1987-08-25 Technographics Fitchburg Coated Products, Inc. Method for the production of abrasion-resistant decorative laminates
US5134070A (en) * 1990-06-04 1992-07-28 Casnig Dael R Method and device for cell cultivation on electrodes
US5632957A (en) * 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
JP3359370B2 (en) 1993-03-17 2002-12-24 光弘 清水 Electrophoresis gel for separation and recovery and separation and recovery method using the same
WO1994029069A1 (en) * 1993-06-04 1994-12-22 Seiko Epson Corporation Apparatus and method for laser machining, and liquid crystal panel
JP3414444B2 (en) * 1993-06-08 2003-06-09 倭 窪田 Immobilization device, method for immobilizing and culturing biological tissue using the same
JPH089966A (en) * 1994-06-30 1996-01-16 Sumitomo Bakelite Co Ltd Method for transporting animal cell
WO1998015813A1 (en) * 1996-10-09 1998-04-16 Symyx Technologies Infrared spectroscopy and imaging of libraries
AU2460399A (en) * 1998-01-20 1999-08-02 Packard Bioscience Company Gel pad arrays and methods and systems for making them
JPH11210750A (en) 1998-01-29 1999-08-03 Koyo Seiko Co Ltd Controlled magnetic bearing device
US6103528A (en) * 1998-04-17 2000-08-15 Battelle Memorial Institute Reversible gelling culture media for in-vitro cell culture in three-dimensional matrices
US6139831A (en) * 1998-05-28 2000-10-31 The Rockfeller University Apparatus and method for immobilizing molecules onto a substrate
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US6488872B1 (en) * 1999-07-23 2002-12-03 The Board Of Trustees Of The University Of Illinois Microfabricated devices and method of manufacturing the same
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JP2003102465A (en) 2001-09-27 2003-04-08 Precision System Science Co Ltd Method for isolating target substance

Also Published As

Publication number Publication date
JPWO2003095088A1 (en) 2005-09-08
CA2476286C (en) 2008-03-18
WO2003095088A1 (en) 2003-11-20
US20050208465A1 (en) 2005-09-22
EP1504812B1 (en) 2014-01-01
US7824854B2 (en) 2010-11-02
EP1504812A4 (en) 2006-08-23
EP1504812A1 (en) 2005-02-09
JP4228114B2 (en) 2009-02-25

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EEER Examination request
MKLA Lapsed

Effective date: 20160114