CA2554060C - Electrochemical biosensor - Google Patents
Electrochemical biosensor Download PDFInfo
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- CA2554060C CA2554060C CA2554060A CA2554060A CA2554060C CA 2554060 C CA2554060 C CA 2554060C CA 2554060 A CA2554060 A CA 2554060A CA 2554060 A CA2554060 A CA 2554060A CA 2554060 C CA2554060 C CA 2554060C
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- counter electrode
- resistance portion
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3272—Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
- C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3274—Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/4163—Systems checking the operation of, or calibrating, the measuring apparatus
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T29/00—Metal working
- Y10T29/49—Method of mechanical manufacture
- Y10T29/49002—Electrical device making
- Y10T29/49082—Resistor making
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T29/00—Metal working
- Y10T29/49—Method of mechanical manufacture
- Y10T29/49002—Electrical device making
- Y10T29/49082—Resistor making
- Y10T29/49099—Coating resistive material on a base
Abstract
According to the present invention, an electrochemical sensor (10) for detecting the concentration of analyte in a fluid test sample is disclosed. The sensor (10) includes a counter electrode having a high-resistance portion (19) for use in detecting whether a predetermined amount of sample has been received by the test sensor.
Description
ELECTROCHEMICAL BIOSENSOR
FIELD OF THE INVENTION
[001] The present invention is generally related to an electrochemical biosensor for use in the quantification of an analyte in a liquid sample and, more particularly, to a system for detecting an insufficient sample amount in an electrochemical biosensor.
BACKGROUND OF THE INVENTION
FIELD OF THE INVENTION
[001] The present invention is generally related to an electrochemical biosensor for use in the quantification of an analyte in a liquid sample and, more particularly, to a system for detecting an insufficient sample amount in an electrochemical biosensor.
BACKGROUND OF THE INVENTION
[002] Medical conditions such as diabetes require a person afflicted with the condition to regularly self-monitor that person's blood-glucose concentration level.
The purpose of monitoring the blood glucose concentration level is to determine the person's blood glucose concentration level and then to take corrective action, based upon whether the level is too high or too low, to bring the level back within a normal range. The failure to take corrective action can have serious medical implications for that person.
The purpose of monitoring the blood glucose concentration level is to determine the person's blood glucose concentration level and then to take corrective action, based upon whether the level is too high or too low, to bring the level back within a normal range. The failure to take corrective action can have serious medical implications for that person.
[003] One method of monitoring a person's blood glucose level is with a portable testing device. The portable nature of these devices enables users to conveniently test their blood glucose levels wherever they may be. One type of device utilizes an electrochemical biosensor to harvest the blood sample and to analyze the blood sample. The electrochemical biosensor includes a reagent designed to react with glucose in the blood to create an oxidation current at electrodes disposed within the electrochemical biosensor-this current is indicative of the user's blood glucose concentration level.
[004] A predetermined amount of reagent is included within an electrochemical biosensor, and is designed to react with a predetermined sample volume. If a less-than required sample volume is harvested by the electrochemical biosensor-a condition referred to as being under-filled-an erroneous measurement may result. Because electrochemical biosensors are commonly used in a self-testing environment, there exists an increased chance that an inappropriate amount of sample may be collected. Further, because the sample volumes are very small (typically less than about 10 l) it is difficult for a user to visually determine whether an appropriate amount of sample has been harvested for analysis. Thus, there exists a need for an electrochemical biosensor that reliably detects and alerts a user to the occurrence of an under-filled condition.
SUMMARY OF THE INVENTION
SUMMARY OF THE INVENTION
[005] According to one embodiment of the present invention, an electrochemical sensor for detecting the concentration of analyte in a fluid test sample is disclosed. The sensor includes a counter electrode having a high-resistance portion for use in detecting whether a predetermined amount of sample has been received by the test sensor.
[006] According to another embodiment of the present invention, a method for evaluating whether an electrochemical test sensor is properly filled is disclosed.
The test sensor includes a working electrode coupled to a first lead and a counter electrode coupled to a second lead. The counter electrode includes a high-resistance portion and a low-resistance portion. The test sensor includes a reagent disposed on the working electrode that is adapted to react with an analyte in a fluid sample for producing an electrochemical reaction indicative of the concentration of the analyte in the fluid sample. The method comprises applying a voltage profile across the first and second leads, measuring the current profile at the first and second leads in response to the applied voltage profile, and generating an under-filled error signal when the measured current profile does not have a predetermined profile.
The test sensor includes a working electrode coupled to a first lead and a counter electrode coupled to a second lead. The counter electrode includes a high-resistance portion and a low-resistance portion. The test sensor includes a reagent disposed on the working electrode that is adapted to react with an analyte in a fluid sample for producing an electrochemical reaction indicative of the concentration of the analyte in the fluid sample. The method comprises applying a voltage profile across the first and second leads, measuring the current profile at the first and second leads in response to the applied voltage profile, and generating an under-filled error signal when the measured current profile does not have a predetermined profile.
[007] The above summary of the present invention is not intended to represent each embodiment, or every aspect, of the present invention.
Additional features and benefits of the present invention are apparent from the detailed description, figures, and embodiments set forth below.
BRIEF DESCRIPTION OF THE DRAWINGS
Additional features and benefits of the present invention are apparent from the detailed description, figures, and embodiments set forth below.
BRIEF DESCRIPTION OF THE DRAWINGS
[008] FIG. 1 is an exploded view of an electrochemical biosensor according to one embodiment of the present invention.
[009] FIG. 2a is an oversized top view of an electrode pattern of the electrochemical biosensor of FIG. 1.
[0010] FIG. 2b is a circuitry schematic of the electrochemical biosensor of FIG. 2a when the electrochemical biosensor is partially filled with liquid sample.
[0011] FIG. 2c is a circuitry schematic of the electrochemical biosensor of FIG. 2a when the electrochemical biosensor is appropriately filled with liquid sample.
[0012] FIG. 3a is a plot of the voltage profile applied to the test sensor of FIG.
1 according to one embodiment of the present invention.
1 according to one embodiment of the present invention.
[0013] FIGS. 3b and 3c are plots of the current profile of the test sensor in response to the voltage profile of FIG. 3a in an under-filled condition and an appropriately-filled condition, respectively.
[0014] FIG. 4a is a plot of the voltage profile applied to the test sensor of FIG.
1 according to another embodiment of the present invention.
1 according to another embodiment of the present invention.
[0015] FIGS. 4b and 4c are plots of the current profile of the test sensor in response to the voltage profile of FIG. 4a in an under-filled condition and an appropriately-filled condition, respectively.
[0016] While the invention is susceptible to various modifications and alternative forms, specific embodiments are shown by way of example in the drawings and are described in detail herein. It should be understood, however, that the invention is not intended to be limited to the particular forms disclosed.
DETAILED DESCRIPTION OF THE ILLUSTRATED EMBODIMENTS
DETAILED DESCRIPTION OF THE ILLUSTRATED EMBODIMENTS
[0017] Turning to the drawings and initially to FIG. 1, the construction of an electrochemical sensor 10 is shown according to one embodiment of the present invention. The sensor 10 comprises an insulating base 12 upon which is printed in sequence (typically by screen printing techniques) an electrical conductor pattern including first and second leads 14a,b, an electrode pattern including a working electrode 16, a counter electrode, an insulating (dielectric) layer 20 including an opening 22 and a channel 25, and a reaction layer 24. The counter electrode includes a low-resistance counter electrode branch 18 (LRC electrode) and a high-resistance counter electrode branch 19 (HRC electrode).
[0018] The reaction layer 24 includes a reagent for converting an analyte of interest (e.g., glucose) in a fluid test sample (e.g., blood) into a chemical species that is electrochemically measurable, in terms of the electrical current it produces, by the components of the electrode pattern. The reagent of the reaction layer 24 typically contains an enzyme such as, for example, glucose oxidase, which reacts with the analyte and with an electron acceptor such as a ferricyanide salt to produce an electrochemically measurable species that can be detected by the electrode pattern 16,18,19. The reaction layer 24 comprises a polymer, an enzyme, and an electron acceptor. The reaction layer 24 also includes additional ingredients such as a buffer and a surfactant in some embodiments of the present invention. The reaction layer 24 is disposed over the opening 22 and channel 25 in the insulating layer 20.
Thus, the portion of the reaction layer 24 exposed to the electrode pattern 16,18,19 is defined by an opening 22 and a channel 25 in the insulating layer 20. The working electrode 16 is electrically coupled to the first lead 14a, and the LRC electrode 18 and HRC
electrode 19 are electrically coupled to a second lead 14b.
Thus, the portion of the reaction layer 24 exposed to the electrode pattern 16,18,19 is defined by an opening 22 and a channel 25 in the insulating layer 20. The working electrode 16 is electrically coupled to the first lead 14a, and the LRC electrode 18 and HRC
electrode 19 are electrically coupled to a second lead 14b.
[0019] The reaction layer 24 covers only the working electrode 16, covers the working electrode 16 and the LRC electrode 18, or covers the working electrode 16, the LRC electrode 18, and the HRC electrode 19 in alternative embodiments of the present invention. When the reaction layer 24 covers only the working electrode 16, an electroactive material is present on the LRC electrode 18 to allow it to function as a counter electrode as is well known in the art.
[0020] The sensor 10 includes a lid 30 having a concave portion 32 that forms a capillary channel when mated with the insulating layer 20 for moving the liquid sample from an inlet 34 into the test sensor 10. The downstream end of the capillary channel includes one or more openings 36 for venting the capillary channel-the fluid sample flows from the inlet 34 into the sensor 10 toward the opening 36. In use, the sensor 10 collects a fluid sample (e.g., a blood sample from a patient's finger) by bringing the capillary channel inlet 34 into contact with the fluid sample.
[0021] Referring to FIG. 2a, the working electrode 16 and LRC electrode 18 are configured in a manner such that the LRC electrode 18 is located downstream (in terms of the direction of fluid sample flow along the flow path) from the working electrode 16. This configuration offers the advantage of requiring the test fluid to completely cover the working electrode 16 in order to contact the LRC
electrode 18.
However, the HRC electrode 19, which is coupled to the LRC electrode 18 via a resistor 40, is positioned upstream from the working electrode 16. According to one embodiment of the present invention, the resistor 40 has a resistance of about 50 kS) to about 500 kQ. In other embodiments, the resistance of the resistor 40 ranges between about 250 kQ to about 350 kS2. In yet another embodiment, the resistor has a resistance of about 300 M. The resistor 40 may be screen-printed on the insulating base 12 in a manner similar to the working electrode 16, the LRC
electrode 18, the HRC electrode 19, and the leads 14a,b. Generally, as described below, the resistor 40 is used in detecting an under-filled condition in the test sensor 10, which can result in an inaccurate measurement of the analyte of interest in the fluid sample.
electrode 18.
However, the HRC electrode 19, which is coupled to the LRC electrode 18 via a resistor 40, is positioned upstream from the working electrode 16. According to one embodiment of the present invention, the resistor 40 has a resistance of about 50 kS) to about 500 kQ. In other embodiments, the resistance of the resistor 40 ranges between about 250 kQ to about 350 kS2. In yet another embodiment, the resistor has a resistance of about 300 M. The resistor 40 may be screen-printed on the insulating base 12 in a manner similar to the working electrode 16, the LRC
electrode 18, the HRC electrode 19, and the leads 14a,b. Generally, as described below, the resistor 40 is used in detecting an under-filled condition in the test sensor 10, which can result in an inaccurate measurement of the analyte of interest in the fluid sample.
[0022] Referring to FIG 2b, the working electrode 16 and HRC electrode 19 fonn the circuit illustrated if the sensor 10 is under-filled (i.e., the LRC
electrode 18 in FIG. 2a is not covered by the fluid sample). In this situation, the sensor current passes through the resistor 40. Thus, the potential V2 between the working electrode 16 and the HRC electrode 19 is about the difference between the potential Vl applied to the sensor leads 14a,b and the voltage drop V, across the resistor 40, assuming a negligible resistance along the electrode/lead pattern.
electrode 18 in FIG. 2a is not covered by the fluid sample). In this situation, the sensor current passes through the resistor 40. Thus, the potential V2 between the working electrode 16 and the HRC electrode 19 is about the difference between the potential Vl applied to the sensor leads 14a,b and the voltage drop V, across the resistor 40, assuming a negligible resistance along the electrode/lead pattern.
[0023] Referring to FIG. 2c, the working electrode 16 and the LRC electrode 18 form the illustrated circuit if the sensor 10 is appropriately filled (i.e., the LRC
electrode 18 in FIG. 2a is covered by the liquid sample). In this situation, the resistor 40 is electrically bypassed in the circuitry. Thus, the potential V2 between the working electrode 16 and the LRC electrode 18 is substantially the same as the potential Vi applied to the leads 14a,b of the sensor 10, assuming a negligible resistance along the electrode/lead pattern. The current measured in the sensor 10 is a result of diffusion of electro-active species to the electrodes and the subsequent redox reactions there. For example, at the working electrode 16 an electron is taken from ferrocyanide, oxidizing it to ferricyanide. At the LRC electrode 18 (or at the HRC electrode 19 in an under-filled situation), an electron is added to ferricyanide, reducing it to ferrocyanide. The flow of electrons in the electrical pattern connecting the two electrodes is measured and is related to the amount of ferrocyanide and hence to the amount of glucose in the sample. In normal operation, a relatively high electrical potential, V2 in FIG. 2c, is applied between the electrodes (e.g., about 400 mV), making the oxidation and reduction reactions at the electrodes fast and depleting the region around the working electrode 16 of the reduced mediator (e.g., ferrocyanide). Thus, the current is not constant but decays with time as the reaction is limited by the diffusion to the electrode surface of the reduced mediator. In general, such decaying current i can be described according to equation (1):
i=C=G=t-k (1) In equation (1), C is a constant, G is the concentration of the analyte (e.g., glucose) in the liquid sample, t is the time elapsed since the potential V2 is applied, and k is a constant relating to the current decay profile.
electrode 18 in FIG. 2a is covered by the liquid sample). In this situation, the resistor 40 is electrically bypassed in the circuitry. Thus, the potential V2 between the working electrode 16 and the LRC electrode 18 is substantially the same as the potential Vi applied to the leads 14a,b of the sensor 10, assuming a negligible resistance along the electrode/lead pattern. The current measured in the sensor 10 is a result of diffusion of electro-active species to the electrodes and the subsequent redox reactions there. For example, at the working electrode 16 an electron is taken from ferrocyanide, oxidizing it to ferricyanide. At the LRC electrode 18 (or at the HRC electrode 19 in an under-filled situation), an electron is added to ferricyanide, reducing it to ferrocyanide. The flow of electrons in the electrical pattern connecting the two electrodes is measured and is related to the amount of ferrocyanide and hence to the amount of glucose in the sample. In normal operation, a relatively high electrical potential, V2 in FIG. 2c, is applied between the electrodes (e.g., about 400 mV), making the oxidation and reduction reactions at the electrodes fast and depleting the region around the working electrode 16 of the reduced mediator (e.g., ferrocyanide). Thus, the current is not constant but decays with time as the reaction is limited by the diffusion to the electrode surface of the reduced mediator. In general, such decaying current i can be described according to equation (1):
i=C=G=t-k (1) In equation (1), C is a constant, G is the concentration of the analyte (e.g., glucose) in the liquid sample, t is the time elapsed since the potential V2 is applied, and k is a constant relating to the current decay profile.
[0024] If a higher electrical potential is applied, no increase in the sensor current is measured, and no change to the decay with time is measured because the sensor current is determined by diffusion to the electrode surface. If a lower electrical potential (e.g., about 200 mV) is applied between the electrodes, the oxidation and reduction reactions are slower, but fast enough that the sensor current remains dependent on diffusion. Eventually at a lower voltage (e.g., less than about 200 mV), local depletion of reduced mediator does not occur and the sensor current ceases to vary with time. Thus, during normal operation of the sensor 10, no change in the current decay profile occurs with time over a range of applied potentials.
[0025] The operation of the test sensor 10 with under-fill detection will be described. If the sensor 10 is under-filled (i.e., less than a requisite amount for the designed reaction) the sample only covers the HRC electrode 19 and at least a portion of the working electrode 16. In this under-filled situation, the HRC electrode serves as the entire counter electrode with a high-resistance due to the resistor 40.
FIG. 2b illustrates the circuit under this condition. Current flow through the resistance 40 causes a potential drop Vr over the resistor 40 and reduces the potential V2 available for the electrochemical reactions. If the resistance is high enough, the potential V2 is reduced to a point where the electrode surface reactions are slow and the current measured between electrode leads 14a and 14b does not decay normally with time but is essentially flat. This flat equilibrium current is a dynamic balance between the sensor current and voltage drop Vr on the resistor. Changing the applied voltage V1 changes this equilibrium current-a lower voltage results in a lower equilibrium or steady-state current and a higher voltage results in a higher current.
The sensor current has a "step" profile if a step-shaped voltage profile is applied.
FIG. 2b illustrates the circuit under this condition. Current flow through the resistance 40 causes a potential drop Vr over the resistor 40 and reduces the potential V2 available for the electrochemical reactions. If the resistance is high enough, the potential V2 is reduced to a point where the electrode surface reactions are slow and the current measured between electrode leads 14a and 14b does not decay normally with time but is essentially flat. This flat equilibrium current is a dynamic balance between the sensor current and voltage drop Vr on the resistor. Changing the applied voltage V1 changes this equilibrium current-a lower voltage results in a lower equilibrium or steady-state current and a higher voltage results in a higher current.
The sensor current has a "step" profile if a step-shaped voltage profile is applied.
[0026] In the situation where the sensor 10 is appropriately filled, the sample covers the LRC electrode 18, in addition to the HRC electrode 19, and the working electrode 16. FIG. 2c illustrates the circuitry under this condition. The branch of the circuitry between the HRC electrode 19 and the resistor 40 to the lead 14b is electrically bypassed by the direct connection between the LRC electrode 18 and the lead 14b. The working electrode 16 and the LRC electrode 18 form a low-resistance circuit, and the sensor current has a decay-type profile where the current is limited by diffusion of electro-active species to the electrode surface as described above.
[0027] The present invention provides an electrochemical sensor in which the electrodes are configured so that in the event of an under-filled condition, the result is a current response with time and/or applied voltage that is characteristic, and can be distinguished from the response of a correctly-filled sensor. Specifically, there are at least two ways in which to distinguish a partially-filled sensor 10 from a sensor 10 that is appropriately filled according to alternative embodiments of the present invention. First, the sensor current of a partially-filled sensor 10 does not decay normally with time, unlike the sensor current of an appropriately-filled sensor 10.
Second, the sensor current of a partially-filled test sensor 10 increases with applied voltage due to the resistor 40, while the sensor current of an appropriately-filled sensor 10 (which bypasses the resistor) does not.
[00281 Thus, when the amount of test fluid that enters the capillary space of the test sensor 10 is sufficient only to cover the HRC electrode 19 and at least a portion of the working electrode 16, and when a suitable potential is applied, the current measured across leads 14a,b is essentially constant and not decay normally with time. Put another way, a device coupled to the leads 14a,b senses certain characteristics of the sensor current over time, which are used to determine if an under-filled error condition has occurred. This is accomplished by algorithmically programming the device to detect the under-filled condition by measuring the current at definite time periods after the test fluid has electrically connected the HRC
electrode 19 with the working electrode 16, and/or after the test fluid has electrically connected the working electrode 16 with the LRC electrode 18.
[0029] Referring to FIGS. 3a, 3b, and 3c, one method for determining whether the test sensor 10 is appropriately filled will be described. At time to, a voltage step is applied between the leads 14a,b and held constant until time t1, this period is referred to as the buns period. Next, no voltage (e.g., an open circuit) is applied during a wait period from time ti to time t2. Finally, the voltage step is again applied during a read period from t2 to U. According to one embodiment of the present invention, the burn, wait, and read periods are each about 2 to about 10 seconds in duration. The applied step voltage is about 0.3 Volts to about 0.4 Volts, according to one embodiment of the present invention.
[0030] An under-filled sensor 10 generates a flat sensor current profile during the read period as shown, for example, in FIG. 3b. An appropriately-filled sensor 10 generates a typical decay-type sensor current profile during the read period as shown, for example, in FIG. 3c.
[0031] The decay factor, k, during the read period-from time t2 to t4-is calculated from the two currents, Ira and Ir4, measured at t3 and t4, according to equation (2):
k = ln(I,.3) - ln(Ii.4) (2) ln(t4) - ln(t3 ) In equation (2), the decay factor, k, describes how fast the current i decays in equation (1), where C is a constant, G is the glucose concentration, and t is the time elapsed after the voltage is initially applied. In an appropriately-filled sensor 10, k is typically between about 0.30 and about 0.49, decreasing as glucose concentration increases.
The decay factor drops to zero in under-filled conditions. Therefore, an under-filled sensor 10 is detected by checking if the decay factor is below a pre-determined lower limit.
[0032] Referring to FIGS. 4a, 4b, and 4c, another method for determining whether the test sensor 10 is appropriately filled will be described. A first voltage is applied during the bum period occurring from time to to time t1, and a second higher voltage is applied until time t2. No voltage is applied (e.g., an open circuit) during the wait period from time t2 to time t3. Finally, a voltage is applied during the read period from time t3 to time t5. According to one embodiment, the first voltage applied during the burn period from time to to ti is about 0.3 V, and the second applied during the bum period from time ti to time t2 is about 0.6 V. During the read period, a voltage of about 0.3 V is applied. The bum, wait, and read periods are each about 2 seconds to about 10 seconds in length, with the first voltage of the burn period applied for about 25% to about 75% of the total burn period, according to one embodiment of the present invention.
[0033] An under-filled sensor 10 generates a step sensor current profile lb during the burn period as shown, for example, in FIG. 4b. An appropriately-filled sensor 10 generates a decay-shaped sensor current profile as shown, for example, in FIG. 4c.
(0034] The decay factor k during the bum period is calculated from the two currents, 41 and Ib2, measured at t1 and t2, respectively, according to equation (3):
k ln(Ib1)-ln(Ie2) (3) ln(t2)-ln(t,) During the burn period the decay factor is greater than about 0.2 in an appropriately-filled sensor, but drops below about -1.0 in an under-filled condition. Thus, an under-filled condition is detected by comparing the actual decay factor to a pre-determined lower limit during the burn period.
[0035] According to alternative embodiments, the two algorithms---equations (2) and (3)-for detecting an under-filled condition discussed in connection with FIGS. 3a-c and 4a -c are used jointly to determine whether an under-filled condition has occurred. The decay factor is first evaluated during the burn period as described in connection with FIGS. 4a-c. If no under-filled condition is determined, the decay factor is then evaluated during the read period as described in connection with FIGS.
3a-c. If no under-filled condition is detected during the burn and read periods, an appropriately-filled condition is deemed to have occurred.
[0036] While the invention is susceptible to various modifications and alternative forms, specific embodiments thereof are shown by way of example in the drawings and described in detail herein. It should be understood, however, that it is not intended to limit the invention to the particular forms disclosed, but on the contrary, the intention is to cover all modifications, equivalents, and alternatives .
Second, the sensor current of a partially-filled test sensor 10 increases with applied voltage due to the resistor 40, while the sensor current of an appropriately-filled sensor 10 (which bypasses the resistor) does not.
[00281 Thus, when the amount of test fluid that enters the capillary space of the test sensor 10 is sufficient only to cover the HRC electrode 19 and at least a portion of the working electrode 16, and when a suitable potential is applied, the current measured across leads 14a,b is essentially constant and not decay normally with time. Put another way, a device coupled to the leads 14a,b senses certain characteristics of the sensor current over time, which are used to determine if an under-filled error condition has occurred. This is accomplished by algorithmically programming the device to detect the under-filled condition by measuring the current at definite time periods after the test fluid has electrically connected the HRC
electrode 19 with the working electrode 16, and/or after the test fluid has electrically connected the working electrode 16 with the LRC electrode 18.
[0029] Referring to FIGS. 3a, 3b, and 3c, one method for determining whether the test sensor 10 is appropriately filled will be described. At time to, a voltage step is applied between the leads 14a,b and held constant until time t1, this period is referred to as the buns period. Next, no voltage (e.g., an open circuit) is applied during a wait period from time ti to time t2. Finally, the voltage step is again applied during a read period from t2 to U. According to one embodiment of the present invention, the burn, wait, and read periods are each about 2 to about 10 seconds in duration. The applied step voltage is about 0.3 Volts to about 0.4 Volts, according to one embodiment of the present invention.
[0030] An under-filled sensor 10 generates a flat sensor current profile during the read period as shown, for example, in FIG. 3b. An appropriately-filled sensor 10 generates a typical decay-type sensor current profile during the read period as shown, for example, in FIG. 3c.
[0031] The decay factor, k, during the read period-from time t2 to t4-is calculated from the two currents, Ira and Ir4, measured at t3 and t4, according to equation (2):
k = ln(I,.3) - ln(Ii.4) (2) ln(t4) - ln(t3 ) In equation (2), the decay factor, k, describes how fast the current i decays in equation (1), where C is a constant, G is the glucose concentration, and t is the time elapsed after the voltage is initially applied. In an appropriately-filled sensor 10, k is typically between about 0.30 and about 0.49, decreasing as glucose concentration increases.
The decay factor drops to zero in under-filled conditions. Therefore, an under-filled sensor 10 is detected by checking if the decay factor is below a pre-determined lower limit.
[0032] Referring to FIGS. 4a, 4b, and 4c, another method for determining whether the test sensor 10 is appropriately filled will be described. A first voltage is applied during the bum period occurring from time to to time t1, and a second higher voltage is applied until time t2. No voltage is applied (e.g., an open circuit) during the wait period from time t2 to time t3. Finally, a voltage is applied during the read period from time t3 to time t5. According to one embodiment, the first voltage applied during the burn period from time to to ti is about 0.3 V, and the second applied during the bum period from time ti to time t2 is about 0.6 V. During the read period, a voltage of about 0.3 V is applied. The bum, wait, and read periods are each about 2 seconds to about 10 seconds in length, with the first voltage of the burn period applied for about 25% to about 75% of the total burn period, according to one embodiment of the present invention.
[0033] An under-filled sensor 10 generates a step sensor current profile lb during the burn period as shown, for example, in FIG. 4b. An appropriately-filled sensor 10 generates a decay-shaped sensor current profile as shown, for example, in FIG. 4c.
(0034] The decay factor k during the bum period is calculated from the two currents, 41 and Ib2, measured at t1 and t2, respectively, according to equation (3):
k ln(Ib1)-ln(Ie2) (3) ln(t2)-ln(t,) During the burn period the decay factor is greater than about 0.2 in an appropriately-filled sensor, but drops below about -1.0 in an under-filled condition. Thus, an under-filled condition is detected by comparing the actual decay factor to a pre-determined lower limit during the burn period.
[0035] According to alternative embodiments, the two algorithms---equations (2) and (3)-for detecting an under-filled condition discussed in connection with FIGS. 3a-c and 4a -c are used jointly to determine whether an under-filled condition has occurred. The decay factor is first evaluated during the burn period as described in connection with FIGS. 4a-c. If no under-filled condition is determined, the decay factor is then evaluated during the read period as described in connection with FIGS.
3a-c. If no under-filled condition is detected during the burn and read periods, an appropriately-filled condition is deemed to have occurred.
[0036] While the invention is susceptible to various modifications and alternative forms, specific embodiments thereof are shown by way of example in the drawings and described in detail herein. It should be understood, however, that it is not intended to limit the invention to the particular forms disclosed, but on the contrary, the intention is to cover all modifications, equivalents, and alternatives .
Claims (52)
1. An electrochemical sensor for detecting the concentration of an analyte in a fluid test sample, the sensor comprising:
a flow path for receiving the fluid test sample;
a first lead and a second lead each adapted to be electrically coupled with a detector of electrical current;
a working electrode disposed along the flow path, the working electrode being in electrical communication with the first lead;
a counter electrode in electrical communication with the second lead, the counter electrode having a low-resistance portion and a high-resistance portion, the low-resistance portion of the counter electrode being disposed along the flow path downstream from the working electrode, the high-resistance portion of the counter electrode being disposed along the flow path upstream from the working electrode, the high-resistance portion including a resistor; and a reagent disposed on the working electrode, the reagent being adapted to react with the analyte to produce electrons that are transferred to the working electrode;
wherein a first current profile is produced at the first and second leads in response to a voltage profile applied to the first and second leads when electrical communication occurs between only the high-resistance portion of the counter electrode and the working electrode, a second current profile is produced at the first and second leads in response to substantially the same voltage profile applied to the first and second leads when electrical communication occurs between the low-resistance and high-resistance portions of the counter electrodes and the working electrode, the first current profile being different than the second current profile.
a flow path for receiving the fluid test sample;
a first lead and a second lead each adapted to be electrically coupled with a detector of electrical current;
a working electrode disposed along the flow path, the working electrode being in electrical communication with the first lead;
a counter electrode in electrical communication with the second lead, the counter electrode having a low-resistance portion and a high-resistance portion, the low-resistance portion of the counter electrode being disposed along the flow path downstream from the working electrode, the high-resistance portion of the counter electrode being disposed along the flow path upstream from the working electrode, the high-resistance portion including a resistor; and a reagent disposed on the working electrode, the reagent being adapted to react with the analyte to produce electrons that are transferred to the working electrode;
wherein a first current profile is produced at the first and second leads in response to a voltage profile applied to the first and second leads when electrical communication occurs between only the high-resistance portion of the counter electrode and the working electrode, a second current profile is produced at the first and second leads in response to substantially the same voltage profile applied to the first and second leads when electrical communication occurs between the low-resistance and high-resistance portions of the counter electrodes and the working electrode, the first current profile being different than the second current profile.
2. The sensor according to claim 1 wherein the second current profile has a decay-type shape.
3. The sensor according to any one of claims 1-2, wherein the resistor has a resistance of about 50 k.OMEGA. to about 500 k.OMEGA..
4. The sensor according to any one of claims 1-2, wherein the resistor has a resistance of about 250 k.OMEGA. to about 350 k.OMEGA..
5. The sensor according to any one of claims 1-4, wherein electrical communication occurs between only the high-resistance portion of the counter electrode and the working electrode when a less than predetermined volume of fluid sample is received by the flow path.
6. The sensor according to any one of claims 1-4, wherein electrical communication occurs between the low-resistance portion of the counter electrode and the working electrode when at least a predetermined volume of fluid sample is received by the flow path.
7. The sensor according to any one of claims 1-6, wherein the first current profile and the voltage profile have similar shapes when electrical communication occurs between only the high-resistance portion of the counter electrode and the working electrode.
8. The test sensor according to any one of claims 1-7, wherein the first and second leads are electrically coupled to the detector of electrical current, the detector being adapted to generate an under-filled error signal when a current profile produced at the first and second leads in response to a voltage profile applied to the first and second leads does not have a decay-type shape.
9. The test sensor according to any one of claims 1-8, wherein the fluid test sample comprises blood.
10. The test sensor according to any one of claims 1-9, wherein the analyte comprises glucose.
11. The test sensor according to any one of claims 1-10, wherein the reagent comprises glucose oxidase.
12. The test sensor according to any one of claims 1-11, further comprising a reaction layer that includes the reagent, the reaction layer covering the working electrode and the low-resistance portion of the counter electrode.
13. A method for evaluating whether an electrochemical test sensor is properly filled, the test sensor including a working electrode electrically coupled to a first lead and a counter electrode electrically coupled to a second lead, the counter electrode including a low-resistance portion and a high-resistance portion, the high-resistance portion including a resistor, the method comprising:
applying a voltage profile across the first and second leads;
measuring the current profile at the first and second leads in response to the applied voltage profile;
comparing the measured current profile to a predetermined profile; and generating an under-filled error signal based on the comparing of the measured current profile to the predetermined profile.
applying a voltage profile across the first and second leads;
measuring the current profile at the first and second leads in response to the applied voltage profile;
comparing the measured current profile to a predetermined profile; and generating an under-filled error signal based on the comparing of the measured current profile to the predetermined profile.
14. The method according to claim 13 wherein the predetermined profile is a decay-shaped profile.
15. The method according to any one of claims 13-14, further comprising forming an electrical communication between only the high-resistance portion of the counter electrode and the working electrode when the test sensor is under-filled.
16. The method according to any one of claims 13-14, further comprising forming an electrical communication between the low-resistance and high-resistance portions of the counter electrode and the working electrode when the test sensor is appropriately-filled.
17. The method according to any one of claims 13-16, wherein the fluid test sample comprises blood.
18. The method according to any one of claims 13-17, wherein the analyte comprises glucose.
19. The sensor according to any one of claims 1-12, wherein the resistor is screen printed on an insulating base.
20. The sensor according to any one of claims 1-12 and 19, wherein the reagent is also disposed on the high-resistance portion of the counter electrode.
21. The sensor according to any one of claims 1-12 and 19-20, wherein the reagent is also disposed on the low-resistance portion of the counter electrode.
22. The method according to any one of claims 13-18, wherein the test sensor includes a flow path for receiving the blood sample, the low-resistance portion of the counter electrode is disposed along the flow path downstream from the working electrode, and the high-resistance portion of the counter electrode is disposed along the flow path upstream from the working electrode.
23. The method according to any one of claims 13-18 and 22, wherein the electrochemical test sensor includes a reagent disposed on the working electrode that is adapted to react with glucose in a blood sample for producing an electrochemical reaction indicative of the concentration of the glucose in the blood sample.
24. The method according to claim 23, wherein the reagent is also disposed on the high-resistance portion of the counter electrode.
25. The method according to any one of claims 23-24, wherein the reagent is also disposed on the low-resistance portion of the counter electrode.
26. A method for evaluating whether an electrochemical sensor is properly filled, comprising:
introducing the fluid test sample to the electrochemical test sensor;
applying a first potential between a counter electrode and a working electrode for a first predetermined time period after the fluid test sample has electrically connected the working electrode and the counter electrode;
after the first predetermined time period, applying a second potential between the counter electrode and the working electrode for a second predetermined time period, the second potential having a magnitude that is greater than a magnitude of the first potential;
measuring the current between the counter electrode and the working electrode at a first interval during the first predetermined time period to obtain a first current measurement;
measuring the current between the counter electrode and the working electrode at a second interval during the second predetermined period to obtain a second current measurement;
determining a first parameter based on the first current measurement and the second current measurement;
comparing the first parameter to at least one first predetermined parameter;
and notifying a user that an insufficient quantity of fluid test sample has been introduced to the electrochemical test sensor based on the comparing the first parameter to the at least one first predetermined parameter.
introducing the fluid test sample to the electrochemical test sensor;
applying a first potential between a counter electrode and a working electrode for a first predetermined time period after the fluid test sample has electrically connected the working electrode and the counter electrode;
after the first predetermined time period, applying a second potential between the counter electrode and the working electrode for a second predetermined time period, the second potential having a magnitude that is greater than a magnitude of the first potential;
measuring the current between the counter electrode and the working electrode at a first interval during the first predetermined time period to obtain a first current measurement;
measuring the current between the counter electrode and the working electrode at a second interval during the second predetermined period to obtain a second current measurement;
determining a first parameter based on the first current measurement and the second current measurement;
comparing the first parameter to at least one first predetermined parameter;
and notifying a user that an insufficient quantity of fluid test sample has been introduced to the electrochemical test sensor based on the comparing the first parameter to the at least one first predetermined parameter.
27. The method according to claim 26, wherein the first parameter is determinable by a calculation having the general form first parameter = 1n(I t1) - 1n(I t2) / (1n(t2)-1n(t1)) where I t1 is the first current measurement at the first interval, t1, and I
t2 is the second current measurement at the second interval, t2.
t2 is the second current measurement at the second interval, t2.
28. The method according to any one of claims 26-27, wherein the second current measurement has a magnitude that is greater than a magnitude of the first current measurement when an insufficient quantity of fluid test sample has been introduced to the electrochemical test sensor.
29. The method according to any one of claims 26-28, further comprising:
after the second predetermined time period, applying a third potential between the counter electrode and the working electrode for a third predetermined time period;
measuring the current between the counter electrode and the working electrode at a third interval during the third predetermined time period to obtain a third current measurement;
measuring the current between the counter electrode and the working electrode at a fourth interval during the third predetermined time period to obtain a fourth current measurement;
determining a second parameter based on the third current measurement and the fourth current measurement;
comparing the second parameter to at least one second predetermined parameter;
and notifying a user that an insufficient quantity of fluid test sample has been introduced to the electrochemical test sensor based on the comparing the second parameter to the at least one second predetermined parameter.
after the second predetermined time period, applying a third potential between the counter electrode and the working electrode for a third predetermined time period;
measuring the current between the counter electrode and the working electrode at a third interval during the third predetermined time period to obtain a third current measurement;
measuring the current between the counter electrode and the working electrode at a fourth interval during the third predetermined time period to obtain a fourth current measurement;
determining a second parameter based on the third current measurement and the fourth current measurement;
comparing the second parameter to at least one second predetermined parameter;
and notifying a user that an insufficient quantity of fluid test sample has been introduced to the electrochemical test sensor based on the comparing the second parameter to the at least one second predetermined parameter.
30. The method according to claim 29, wherein the second parameter is determinable by a calculation having the general form second parameter = 1n(I t3) - 1n(I t4) / (1n(t4)-1n(t3)) where I t3 is the third current measurement at the third interval, t3, and I
t4 is the fourth current measurement at the fourth interval, t4.
t4 is the fourth current measurement at the fourth interval, t4.
31. The method according to any one of claims 29-30, wherein the first predetermined time period and the second predetermined time period comprise a burn period, the third predetermined time period comprises a read period, and no potential is applied between the counter electrode and the working electrode for a wait period, the wait period being after the burn period and prior to the read period.
32. The method according to any one of claims 29-30, wherein the first predetermined time period and the second predetermined time period comprise a first pulse period, and the third predetermined time period comprises a second pulse period.
33. A method for evaluating whether an electrochemical sensor is properly filled, the test sensor including a working electrode coupled to a first lead, and counter electrode coupled to a second lead, the counter electrode including a high-resistance portion and a low-resistance portion, the high-resistance portion including a resistor, the method comprising:
providing a blood sample;
applying a first potential across the first and second leads for a first predetermined time period;
after the first predetermined time period, applying a second potential across the first and second leads for a second predetermined time period, the second potential having a magnitude that is greater than a magnitude of the first potential; and measuring the current at the first and second leads at at least one interval in response to the applied first potential and the applied second potential.
providing a blood sample;
applying a first potential across the first and second leads for a first predetermined time period;
after the first predetermined time period, applying a second potential across the first and second leads for a second predetermined time period, the second potential having a magnitude that is greater than a magnitude of the first potential; and measuring the current at the first and second leads at at least one interval in response to the applied first potential and the applied second potential.
34. The method of claim 33, further comprising generating an under-filled error signal based on the measured current.
35. A method for evaluating whether an electrochemical test sensor is properly filled, the test sensor including a working electrode electrically coupled to a first lead and a counter electrode electrically coupled to a second lead, the counter electrode including a low-resistance portion and a high-resistance portion, the high-resistance portion including means for increasing electrical resistance such that the high-resistance portion has a greater electrical resistance than the low-resistance portion, the test sensor including a reagent disposed on the working electrode that is adapted to react with glucose in a blood sample for producing an electrochemical reaction indicative of the concentration of the glucose in the fluid test sample, the method comprising:
providing a fluid test sample;
applying a voltage profile across the first and second leads;
measuring the current profile at the first and second leads in response to the applied voltage profile;
comparing the measured current profile to a predetermined current profile; and generating an under-filled error signal based on the comparing of the measured current profile to the predetermined profile.
providing a fluid test sample;
applying a voltage profile across the first and second leads;
measuring the current profile at the first and second leads in response to the applied voltage profile;
comparing the measured current profile to a predetermined current profile; and generating an under-filled error signal based on the comparing of the measured current profile to the predetermined profile.
36. The method according to claim 35, wherein the voltage profile is step-shaped.
37. The method according to any one of claims 35-36, wherein the predetermined profile is a decay-shaped profile.
38. A method for evaluating a fluid test sample using an electrochemical sensor, the test sensor including a working electrode coupled to a first lead, and counter electrode coupled to a second lead, the counter electrode including a high-resistance portion and a low-resistance portion, the high resistance portion including a resistor, the test sensor including a reagent disposed on the working electrode that is adapted to react with glucose in a blood sample for producing an electrochemical reaction indicative of the concentration of the glucose in the blood sample, the method comprising:
providing a blood sample;
applying a voltage profile across the first and second leads;
measuring the current profile at the first and second leads in response to the applied voltage profile; and determining a decay factor based on the measured current profile.
providing a blood sample;
applying a voltage profile across the first and second leads;
measuring the current profile at the first and second leads in response to the applied voltage profile; and determining a decay factor based on the measured current profile.
39. The method of claim 38, comprising forming an electrical communication between only the high-resistance portion of the counter electrode and the working electrode when the test sensor is under-filled.
40. The method according to any one of claims 38-39, comprising forming an electrical communication between the low-resistance portion of the counter electrode and the working electrode when the test sensor is appropriately-filled.
41. The method according to any one of claims 38-40, wherein the reagent is also disposed on the low-resistance portion of the counter electrode.
42. The method according to any one of claims 38-41, wherein the resistor is screen printed on an insulating base of the test sensor.
43. The method according to any one of claims 38-42, wherein the reagent is also disposed on the high-resistance portion.
44. The method according to any one of claims 38-43, wherein the test sensor includes a flow path for receiving the blood sample, the low-resistance portion of the counter electrode is disposed along the flow path downstream from the working electrode, and the high-resistance portion of the counter electrode is disposed along the flow path upstream from the working electrode.
45. A method of evaluating a fluid test sample that has been introduced to an electrochemical test sensor, the method comprising the acts of:
providing the electrochemical test sensor adapted to assist in determining the concentration of an analyte in a fluid test sample, the sensor comprising a base that assists in forming an opening for introducing the fluid test sample, a working electrode coupled to the base, and a counter electrode coupled to the base, the counter electrode including a low resistance portion and a high resistance portion, the high resistance portion including a resistor;
introducing the fluid test sample to the test sensor;
measuring current at a plurality of time periods to obtain a plurality of current measurements; and determining a decay factor from the plurality of current measurements.
providing the electrochemical test sensor adapted to assist in determining the concentration of an analyte in a fluid test sample, the sensor comprising a base that assists in forming an opening for introducing the fluid test sample, a working electrode coupled to the base, and a counter electrode coupled to the base, the counter electrode including a low resistance portion and a high resistance portion, the high resistance portion including a resistor;
introducing the fluid test sample to the test sensor;
measuring current at a plurality of time periods to obtain a plurality of current measurements; and determining a decay factor from the plurality of current measurements.
46. The method of claim 45, further comprising:
comparing the decay factor with a tolerance range, and generating an under-filled error signal based on the comparing the decay factor with the tolerance range.
comparing the decay factor with a tolerance range, and generating an under-filled error signal based on the comparing the decay factor with the tolerance range.
47. The method of claim 46, wherein the decay factor is outside of the tolerance range when the fluid test sample fails to contact the low-resistance portion of the counter electrode.
48. The sensor according to any one of claims 1-12 and 19-21, wherein the voltage profile is step-shaped.
49. The sensor according to any one of claims 1-12, 19-21 and 48, wherein the first current profile has a step-type shape.
50. The method according to any one of claims 13-18 and 22-25, wherein the voltage profile is step-shaped.
51. The method according to any one of claims 26-32, wherein the magnitude of the first potential is about 0.3 volts.
52. The method according to any one of claims 26-32 and 51, wherein the magnitude of the second potential is about 0.6 volts.
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2005
- 2005-02-04 CA CA2554060A patent/CA2554060C/en not_active Expired - Fee Related
- 2005-02-04 JP JP2006552370A patent/JP4814110B2/en active Active
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- 2005-02-04 CN CN2005800042770A patent/CN1918471B/en active Active
- 2005-02-04 WO PCT/US2005/004226 patent/WO2005078437A1/en active Application Filing
- 2005-02-04 AT AT05713272T patent/ATE532063T1/en active
- 2005-02-04 EP EP05713272A patent/EP1714148B1/en active Active
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US20120132542A1 (en) | 2012-05-31 |
NO20063898L (en) | 2006-09-01 |
US20110061458A1 (en) | 2011-03-17 |
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CN1918471B (en) | 2010-12-29 |
BRPI0507322A (en) | 2007-06-26 |
US20160274052A1 (en) | 2016-09-22 |
CN1918471A (en) | 2007-02-21 |
US8137529B2 (en) | 2012-03-20 |
EP1714148B1 (en) | 2011-11-02 |
US8388827B2 (en) | 2013-03-05 |
RU2371707C2 (en) | 2009-10-27 |
CA2554060A1 (en) | 2005-08-25 |
US20140202881A1 (en) | 2014-07-24 |
ATE532063T1 (en) | 2011-11-15 |
US9377430B2 (en) | 2016-06-28 |
WO2005078437A1 (en) | 2005-08-25 |
US20130161206A1 (en) | 2013-06-27 |
JP2007523327A (en) | 2007-08-16 |
JP4814110B2 (en) | 2011-11-16 |
EP1714148A1 (en) | 2006-10-25 |
US8702961B2 (en) | 2014-04-22 |
US20070045127A1 (en) | 2007-03-01 |
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