CA2666234C - Haptens, hapten conjugates, compositions thereof and method for their preparation and use - Google Patents

Haptens, hapten conjugates, compositions thereof and method for their preparation and use Download PDF

Info

Publication number
CA2666234C
CA2666234C CA2666234A CA2666234A CA2666234C CA 2666234 C CA2666234 C CA 2666234C CA 2666234 A CA2666234 A CA 2666234A CA 2666234 A CA2666234 A CA 2666234A CA 2666234 C CA2666234 C CA 2666234C
Authority
CA
Canada
Prior art keywords
different
haptens
sample
compositions
conjugated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2666234A
Other languages
French (fr)
Other versions
CA2666234A1 (en
Inventor
Jerry W. Kosmeder
Mark Lefever
Donald Johnson
Michael Farrell
Zhanna Zhilina
Christopher Bieniarz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ventana Medical Systems Inc
Original Assignee
Ventana Medical Systems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ventana Medical Systems Inc filed Critical Ventana Medical Systems Inc
Priority to CA2858359A priority Critical patent/CA2858359C/en
Publication of CA2666234A1 publication Critical patent/CA2666234A1/en
Application granted granted Critical
Publication of CA2666234C publication Critical patent/CA2666234C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D215/00—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems
    • C07D215/02—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom
    • C07D215/16—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D215/48—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen
    • C07D215/50—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen attached in position 4
    • C07D215/52—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen attached in position 4 with aryl radicals attached in position 2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D231/00—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings
    • C07D231/02—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings
    • C07D231/10—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
    • C07D231/14—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D231/16—Halogen atoms or nitro radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D241/00—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings
    • C07D241/36—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings condensed with carbocyclic rings or ring systems
    • C07D241/38—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings condensed with carbocyclic rings or ring systems with only hydrogen or carbon atoms directly attached to the ring nitrogen atoms
    • C07D241/40—Benzopyrazines
    • C07D241/44—Benzopyrazines with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to carbon atoms of the hetero ring
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D243/00—Heterocyclic compounds containing seven-membered rings having two nitrogen atoms as the only ring hetero atoms
    • C07D243/06—Heterocyclic compounds containing seven-membered rings having two nitrogen atoms as the only ring hetero atoms having the nitrogen atoms in positions 1 and 4
    • C07D243/10—Heterocyclic compounds containing seven-membered rings having two nitrogen atoms as the only ring hetero atoms having the nitrogen atoms in positions 1 and 4 condensed with carbocyclic rings or ring systems
    • C07D243/12—1,5-Benzodiazepines; Hydrogenated 1,5-benzodiazepines
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D271/00—Heterocyclic compounds containing five-membered rings having two nitrogen atoms and one oxygen atom as the only ring hetero atoms
    • C07D271/02—Heterocyclic compounds containing five-membered rings having two nitrogen atoms and one oxygen atom as the only ring hetero atoms not condensed with other rings
    • C07D271/08—1,2,5-Oxadiazoles; Hydrogenated 1,2,5-oxadiazoles
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D271/00—Heterocyclic compounds containing five-membered rings having two nitrogen atoms and one oxygen atom as the only ring hetero atoms
    • C07D271/12—Heterocyclic compounds containing five-membered rings having two nitrogen atoms and one oxygen atom as the only ring hetero atoms condensed with carbocyclic rings or ring systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D277/00—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings
    • C07D277/02—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings
    • C07D277/20—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
    • C07D277/32—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D277/38—Nitrogen atoms
    • C07D277/44—Acylated amino or imino radicals
    • C07D277/48—Acylated amino or imino radicals by radicals derived from carbonic acid, or sulfur or nitrogen analogues thereof, e.g. carbonylguanidines
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D277/00—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings
    • C07D277/60—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings condensed with carbocyclic rings or ring systems
    • C07D277/62—Benzothiazoles
    • C07D277/68—Benzothiazoles with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached in position 2
    • C07D277/70—Sulfur atoms
    • C07D277/76—Sulfur atoms attached to a second hetero atom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
    • C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
    • C07D311/06—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 2
    • C07D311/08—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 2 not hydrogenated in the hetero ring
    • C07D311/12—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 2 not hydrogenated in the hetero ring substituted in position 3 and unsubstituted in position 7
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
    • C07D417/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings
    • C07D417/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings linked by a chain containing hetero atoms as chain links
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D491/00—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00
    • C07D491/02—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00 in which the condensed system contains two hetero rings
    • C07D491/04—Ortho-condensed systems
    • C07D491/044—Ortho-condensed systems with only one oxygen atom as ring hetero atom in the oxygen-containing ring
    • C07D491/052—Ortho-condensed systems with only one oxygen atom as ring hetero atom in the oxygen-containing ring the oxygen-containing ring being six-membered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D491/00—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00
    • C07D491/02—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00 in which the condensed system contains two hetero rings
    • C07D491/04—Ortho-condensed systems
    • C07D491/056—Ortho-condensed systems with two or more oxygen atoms as ring hetero atoms in the oxygen-containing ring
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D491/00—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00
    • C07D491/12—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00 in which the condensed system contains three hetero rings
    • C07D491/14—Ortho-condensed systems
    • C07D491/147—Ortho-condensed systems the condensed system containing one ring with oxygen as ring hetero atom and two rings with nitrogen as ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
    • C07D493/02—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains two hetero rings
    • C07D493/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
    • C07D493/12—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains three hetero rings
    • C07D493/14—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D495/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms
    • C07D495/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms in which the condensed system contains two hetero rings
    • C07D495/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D498/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D498/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms in which the condensed system contains two hetero rings
    • C07D498/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/583—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with non-fluorescent dye label
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/961—Chemistry: molecular biology and microbiology including a step of forming, releasing, or exposing the antigen or forming the hapten-immunogenic carrier complex or the antigen per se
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/807—Hapten conjugated with peptide or protein

Abstract

A method for performing a multiplexed diagnostic assay, such as for two or more different targets in a sample, is described. One embodiment comprised contacting the sample with two or more specific binding moieties that bind specifically to two or more different targets. The two or more specific binding moieties are conjugated to different haptens, and at least one of the haptens is an oxazole, a pyrazole, a thiazole, a nitroaryl compound other than dinitrophenyl, a benzofurazan, a triterpene, a urea, a thiourea, a rotenoid, a coumarin, a cyclolignan, a heterobiaryl, an azo aryl, or a benzodiazepine. The sample is contacted with two or more different anti-hapten antibodies that can be detected separately. The two or more different anti-hapten antibodies may be conjugated to different detectable labels.
CA2666234A 2006-11-01 2007-11-01 Haptens, hapten conjugates, compositions thereof and method for their preparation and use Active CA2666234C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2858359A CA2858359C (en) 2006-11-01 2007-11-01 Haptens, hapten conjugates, compositions thereof and method for their preparation and use

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US85613306P 2006-11-01 2006-11-01
US60/856,133 2006-11-01
PCT/US2007/023140 WO2008063378A2 (en) 2006-11-01 2007-11-01 Haptens, hapten conjugates, compositions thereof and method for their preparation and use

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA2858359A Division CA2858359C (en) 2006-11-01 2007-11-01 Haptens, hapten conjugates, compositions thereof and method for their preparation and use

Publications (2)

Publication Number Publication Date
CA2666234A1 CA2666234A1 (en) 2008-05-29
CA2666234C true CA2666234C (en) 2014-10-14

Family

ID=39430271

Family Applications (5)

Application Number Title Priority Date Filing Date
CA2858359A Active CA2858359C (en) 2006-11-01 2007-11-01 Haptens, hapten conjugates, compositions thereof and method for their preparation and use
CA2995467A Active CA2995467C (en) 2006-11-01 2007-11-01 Haptens, hapten conjugates, compositions thereof and method for their preparation and use
CA3125026A Active CA3125026C (en) 2006-11-01 2007-11-01 Haptens, hapten conjugates, compositions thereof and method for their preparation and use
CA3069091A Active CA3069091C (en) 2006-11-01 2007-11-01 Haptens, hapten conjugates, compositions thereof and method for their preparation and use
CA2666234A Active CA2666234C (en) 2006-11-01 2007-11-01 Haptens, hapten conjugates, compositions thereof and method for their preparation and use

Family Applications Before (4)

Application Number Title Priority Date Filing Date
CA2858359A Active CA2858359C (en) 2006-11-01 2007-11-01 Haptens, hapten conjugates, compositions thereof and method for their preparation and use
CA2995467A Active CA2995467C (en) 2006-11-01 2007-11-01 Haptens, hapten conjugates, compositions thereof and method for their preparation and use
CA3125026A Active CA3125026C (en) 2006-11-01 2007-11-01 Haptens, hapten conjugates, compositions thereof and method for their preparation and use
CA3069091A Active CA3069091C (en) 2006-11-01 2007-11-01 Haptens, hapten conjugates, compositions thereof and method for their preparation and use

Country Status (8)

Country Link
US (5) US7695929B2 (en)
EP (7) EP2444807B1 (en)
JP (6) JP5576120B2 (en)
AU (1) AU2007322261B2 (en)
CA (5) CA2858359C (en)
DK (7) DK2442107T3 (en)
ES (7) ES2761949T3 (en)
WO (1) WO2008063378A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9180215B2 (en) 2007-02-07 2015-11-10 Purdue Research Foundation Positron emission tomography imaging method

Families Citing this family (94)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002087424A2 (en) 2001-05-02 2002-11-07 Purdue Research Foundation Treatment and diagnosis of macrophage mediated disease
US8043603B2 (en) 2002-02-07 2011-10-25 Endocyte, Inc. Folate targeted enhanced tumor and folate receptor positive tissue optical imaging technology
US8043602B2 (en) 2002-02-07 2011-10-25 Endocyte, Inc. Folate targeted enhanced tumor and folate receptor positive tissue optical imaging technology
US20070009434A1 (en) 2005-07-05 2007-01-11 Low Philip S Imaging and therapeutic method using monocytes
EP1940473A2 (en) 2005-09-23 2008-07-09 Purdue Research Foundation Multiphoton in vivo flow cytometry method and device
WO2007050811A2 (en) 2005-10-27 2007-05-03 The President And Fellows Of Harvard College Methods and compositions for labeling nucleic acids
WO2008063378A2 (en) 2006-11-01 2008-05-29 Ventana Medical Systems, Inc. Haptens, hapten conjugates, compositions thereof and method for their preparation and use
CA2668197A1 (en) 2006-11-03 2008-05-15 Philip S. Low Ex vivo flow cytometry method and device
US7682789B2 (en) * 2007-05-04 2010-03-23 Ventana Medical Systems, Inc. Method for quantifying biomolecules conjugated to a nanoparticle
EP2167963B1 (en) 2007-05-23 2019-04-17 Ventana Medical Systems, Inc. Polymeric carriers for immunohistochemistry and in situ hybridization
US8961926B2 (en) 2007-05-25 2015-02-24 Purdue Research Foundation Method of imaging localized infections
US9901567B2 (en) 2007-08-01 2018-02-27 Syntarga B.V. Substituted CC-1065 analogs and their conjugates
AU2009256349B2 (en) 2008-06-05 2014-06-26 Ventana Medical Systems, Inc. Compositions comprising nanomaterials and method for using such compositions for histochemical processes
ES2538123T3 (en) 2008-08-22 2015-06-17 Ventana Medical Systems, Inc. Method for chromogenic detection of two or more target molecules in a single sample
CA2755133A1 (en) 2009-03-20 2010-09-23 Amgen Inc. Selective and potent peptide inhibitors of kv1.3
US10191060B2 (en) 2009-11-09 2019-01-29 University Of Washington Functionalized chromophoric polymer dots and bioconjugates thereof
USPP22463P3 (en) * 2010-02-16 2012-01-17 Menachem Bornstein Gypsophila plant named ‘Pearl Blossom’
ES2606012T3 (en) 2010-02-26 2017-03-17 Ventana Medical Systems, Inc. In situ hybridization with PolyTag probes
US10539487B2 (en) 2010-03-04 2020-01-21 Ventana Medical Systems, Inc. Systems and methods for monitoring tissue sample processing
CA2788664C (en) 2010-03-04 2023-04-11 Ventana Medical Systems, Inc. Processing system for processing specimens using acoustic energy
US10126216B2 (en) 2011-02-17 2018-11-13 Ventana Medical Systems, Inc. Method for tissue sample fixation
CA2796087A1 (en) * 2010-04-20 2011-10-27 Ventana Medical Systems, Inc. Two-color chromogenic in situ hybridization
RU2730502C2 (en) 2010-04-21 2020-08-24 Синтарга Б.В. Novel conjugates of analogues of cc-1065 and bifunctional linkers
WO2011139792A2 (en) 2010-04-27 2011-11-10 Ventana Medical Systems, Inc. Antibody-nanoparticle conjugates and methods for making and using such conjugates
US9291597B2 (en) 2010-07-02 2016-03-22 Ventana Medical Systems, Inc. Detecting targets using mass tags and mass spectrometry
WO2012003476A2 (en) * 2010-07-02 2012-01-05 Ventana Medical Systems, Inc. Hapten conjugates for target detection
US8956859B1 (en) 2010-08-13 2015-02-17 Aviex Technologies Llc Compositions and methods for determining successful immunization by one or more vaccines
EP2606055B1 (en) 2010-08-16 2015-06-03 Ventana Medical Systems, Inc. Substrates for chromogenic detection and methods of use in detection assays and kits
MX360946B (en) 2010-09-22 2018-10-29 Amgen Inc Star Carrier immunoglobulins and uses thereof.
CA2814790C (en) 2010-10-18 2019-11-19 University of Washington Center for Commercialization Chromophoric polymer dots
PE20140406A1 (en) 2010-11-18 2014-04-16 Univ Yale BIFUNCTIONAL MOLECULES WITH ANTIBODY RECRUITMENT ACTIVITY AND INITIAL INHIBITORY ACTIVITY AGAINST HUMAN IMMUNODEFICIENCY VIRUS
WO2012123387A1 (en) 2011-03-14 2012-09-20 F. Hoffmann-La Roche Ag A method of analyzing chromosomal translocations and a system therefore
US9797840B2 (en) 2011-11-28 2017-10-24 University Of Washington Through Its Center For Commercialization Highly fluorescent polymer nanoparticle
WO2013079606A1 (en) 2011-12-01 2013-06-06 Ventana Medical Systems, Inc. Automated dual stain of mirna and protein targets
EP2797635B1 (en) 2011-12-30 2020-05-20 University of Washington Through Its Center for Commercialization Chromophoric polymer dots with narrow-band emission
WO2013108126A2 (en) 2012-01-16 2013-07-25 University Of Oslo Methyltransferases and uses thereof
AU2013211666B2 (en) 2012-01-23 2015-09-03 Ventana Medical Systems, Inc Polymer stabilization of chromogen solutions
EP2809510B1 (en) 2012-02-03 2021-03-31 University of Washington through its Center for Commercialization Polyelectrolyte-coated polymer dots and related methods
AU2013240090B2 (en) 2012-03-27 2017-01-05 Ventana Medical Systems, Inc. Signaling conjugates and methods of use
US9123538B2 (en) * 2012-04-26 2015-09-01 Regents Of The University Of Minnesota Silicon nanocrystal inks, films, and methods
CN102654500A (en) * 2012-05-17 2012-09-05 重庆市科学技术研究院 Detecting reagent kit used for detecting quinoxalinone-2-carboxylic acid and method
CN104736564B (en) * 2012-08-21 2019-02-05 詹森药业有限公司 The antibody and application thereof of Quetiapine haptens
EP3764084A1 (en) 2013-03-12 2021-01-13 Ventana Medical Systems, Inc. Digitally enhanced microscopy for multiplexed histology
US9663814B2 (en) 2013-03-12 2017-05-30 Ventana Medical Systems, Inc. Proximity assay for in situ detection of targets
WO2014139979A1 (en) 2013-03-12 2014-09-18 Ventana Medical Systems, Inc. Quantum dot in situ hybridization
EP2972349B1 (en) 2013-03-14 2019-10-23 University Of Washington Through Its Center For Commercialization Polymer dot compositions and related methods
ES2729638T3 (en) * 2013-10-11 2019-11-05 Ventana Med Syst Inc Multiplex assays of joint staining of HER2 and estrogen receptors to detect tumor heterogeneity
WO2015061260A1 (en) 2013-10-24 2015-04-30 Siemens Healthcare Diagnostics Inc. Assays for macromolecular analytes
JP6342517B2 (en) 2014-01-10 2018-06-13 シントン・バイオファーマシューティカルズ・ビー．ブイ．Ｓｙｎｔｈｏｎ Ｂｉｏｐｈａｒｍａｃｅｕｔｉｃａｌｓ Ｂ．Ｖ． Duocarmycin ADC showing improved in vivo anti-tumor activity
MY189713A (en) 2014-01-10 2022-02-28 Univ Yale Duocarmycin adcs for use in treatment of endometrial cancer
EP3110966A1 (en) 2014-02-24 2017-01-04 Ventana Medical Systems, Inc. Automated rna detection using labeled 2'-o-methyl rna oligonucleotide probes and signal amplification systems
ES2713438T3 (en) 2014-02-24 2019-05-21 Ventana Med Syst Inc Signal amplification of quinone analogues
CA2948420A1 (en) 2014-06-06 2015-12-10 Ventana Medical Systems, Inc. Significance of intratumoral her2 heterogeneity in breast cancer and uses therefore
US20170320965A1 (en) * 2014-09-22 2017-11-09 Synthon Biopharmaceuticals B.V. Pan-reactive antibodies to duocarmycins
EP3201628B1 (en) 2014-10-02 2020-11-18 Ventana Medical Systems, Inc. Polymers and conjugates comprising the same
CA2965872C (en) 2014-11-25 2022-02-22 Ventana Medical Systems, Inc. Proximity assays using chemical ligation and hapten transfer
EP3227830B8 (en) 2014-12-03 2020-07-01 Ventana Medical Systems, Inc. Methods, systems, and apparatuses for quantitative analysis of heterogeneous biomarker distribution
EP3253788A4 (en) 2015-02-06 2018-08-08 Cell IDX, Inc. Antigen-coupled immunoreagents
EP3283881B1 (en) 2015-04-13 2022-12-14 Ventana Medical Systems, Inc. Thermochemical-based antibody inactivation methods and systems
EP3283522A1 (en) 2015-04-17 2018-02-21 Spring Bioscience Corporation Antibodies, compositions, and immunohistochemistry methods for detecting c4.4a
JP6711352B2 (en) * 2015-05-28 2020-06-17 コニカミノルタ株式会社 Probe reagents for in situ hybridization
CN105018072B (en) * 2015-07-08 2017-02-01 山东理工大学 Fluorescence probe for detecting human serum albumin and preparation method therefor
JP6880001B2 (en) * 2015-08-28 2021-06-02 ヴェンタナ メディカル システムズ， インク． Protein proximity assay in formalin-fixed paraffin-embedded tissue using caged haptens
CA3016555A1 (en) 2016-03-08 2017-09-14 Ventana Medical Systems, Inc. Multiplexed immunohistochemistry using recombinant antibodies with epitope tags
JP7021126B2 (en) 2016-06-28 2022-02-16 ヴェンタナ メディカル システムズ， インク． New colors for color-developing IHC and ISH staining with multi-dye quinone methide and tyramide conjugates
EP3475291A1 (en) 2016-06-28 2019-05-01 Ventana Medical Systems, Inc. Application of click chemistry for signal amplification in ihc and ish assays
JP2019523000A (en) * 2016-07-18 2019-08-22 セル アイディーエックス， インコーポレイテッド Hybridization reagent coupled with antigen
EP3515449B1 (en) 2016-09-26 2023-07-12 Dana-Farber Cancer Institute, Inc. Quinoline derivatives as chromobox (cbx) protein inhibitors for treating cancer
EP3929304B1 (en) * 2016-12-16 2024-02-21 Aratome LLC Molecular detection using ligation amplification
EP3554556B1 (en) 2016-12-19 2022-03-09 Ventana Medical Systems, Inc. Peptide nucleic acid conjugates
CN110337588B (en) 2016-12-19 2023-02-17 文塔纳医疗系统公司 Method and system for quantitative immunohistochemistry
EP3568157A4 (en) * 2017-01-13 2021-01-06 National Research Council of Canada Method of optimizing peptide immuno-epitope by glycosylation, optimized peptide thereof and its use for conjugate vaccines
CN117551741A (en) 2017-03-31 2024-02-13 乌尔蒂维尤股份有限公司 DNA-antigen exchange and amplification
US10768185B2 (en) * 2017-07-20 2020-09-08 Trustees Of Boston University Tenofovir detection assay
EP3727470A1 (en) 2017-12-18 2020-10-28 Ventana Medical Systems, Inc. Peptide nucleic acid conjugates
US10330684B1 (en) 2018-04-05 2019-06-25 Rarecyte, Inc. Analyte detection
WO2019194994A1 (en) * 2018-04-05 2019-10-10 Rarecyte, Inc. Analyte detection
WO2019224153A1 (en) 2018-05-21 2019-11-28 Genentech, Inc. Her2 heterogeneity as a biomarker in cancer
JP7455816B2 (en) 2018-09-20 2024-03-26 ヴェンタナ メディカル システムズ， インク． Coumarin-based crosslinking reagent
CN113785184A (en) 2019-05-14 2021-12-10 文塔纳医疗系统公司 System comprising a biological sample processing chamber
CN110330488A (en) * 2019-06-20 2019-10-15 深圳市易瑞生物技术股份有限公司 A kind of piroxicam haptens and its preparation method and application
CA3164781A1 (en) 2019-12-18 2021-06-24 F. Hoffmann-La Roche Ag Methods of sequencing by synthesis using a consecutive labeling scheme
US11814677B2 (en) * 2019-12-30 2023-11-14 Arizona Board Of Regents On Behalf Of Arizona State University Methods and systems for sensitive and multiplexed analysis of biological samples using cleavable fluorescent streptavidin and anti-hapten antibodies
WO2021173674A1 (en) 2020-02-26 2021-09-02 A2 Biotherapeutics, Inc. Polypeptides targeting mage-a3 peptide-mhc complexes and methods of use thereof
CN115552248A (en) 2020-05-07 2022-12-30 文塔纳医疗系统公司 Tissue chemistry systems and methods for evaluating EGFR and EGFR ligand expression in tumor samples
KR102506288B1 (en) * 2020-09-07 2023-03-06 국립암센터 A complex comprising an antibody to Digoxigenin and use thereof
CN112362863B (en) * 2020-11-02 2024-02-13 上海市农业科学院 Thiol-functional liposome nano material and preparation method and application thereof
WO2022152777A1 (en) 2021-01-15 2022-07-21 F. Hoffmann-La Roche Ag Storage stable caged haptens
WO2022156920A1 (en) 2021-01-25 2022-07-28 Ventana Medical Systems, Inc. Stained biological specimens including one or more biomarkers labeled with one or more detectable moieties
EP4326736A1 (en) 2021-04-18 2024-02-28 Ventana Medical Systems, Inc. Morphological marker staining
WO2022240641A1 (en) * 2021-05-12 2022-11-17 The Scripps Research Institute High-throughput assay for identification of myc inhibitors
WO2023205669A2 (en) * 2022-04-19 2023-10-26 Purdue Research Foundation Dual and triple hapten conjugates, compositions, processes for making, and methods of treatment therewith
US20230383342A1 (en) * 2022-05-31 2023-11-30 Illumina Cambridge Limited Compositions and methods for nucleic acid sequencing
WO2024002924A2 (en) 2022-06-28 2024-01-04 F. Hoffmann-La Roche Ag Fluorescent dyes with large stokes shift

Family Cites Families (170)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3892530A (en) 1974-09-23 1975-07-01 Hoffmann La Roche Colorimetric and fluorometric method
US4036945A (en) 1976-05-03 1977-07-19 The Massachusetts General Hospital Composition and method for determining the size and location of myocardial infarcts
US4230683A (en) 1978-08-09 1980-10-28 Abbott Laboratories Hapten conjugated antibody for antibody or antigen detection
US4223013A (en) * 1978-12-29 1980-09-16 Syva Company Amitriptyline conjugates to antigenic proteins and enzymes
US4495296A (en) 1979-05-21 1985-01-22 New York Blood Center, Inc. Labeled anti-hapten antibodies and their use as a universal reagent for solid phase radio- and/or enzyme-immunoassays
US4331647A (en) 1980-03-03 1982-05-25 Goldenberg Milton David Tumor localization and therapy with labeled antibody fragments specific to tumor-associated markers
US4425427A (en) 1980-03-13 1984-01-10 Vitafin N.V. Simultaneous, kinetic, spectrophotometric analysis of blood serum for multiple components
US4675189A (en) 1980-11-18 1987-06-23 Syntex (U.S.A.) Inc. Microencapsulation of water soluble active polypeptides
US4469797A (en) 1982-09-23 1984-09-04 Miles Laboratories, Inc. Digoxigenin immunogens, antibodies, labeled conjugates, and related derivatives
US4490473A (en) 1983-03-28 1984-12-25 Panab Labeled antibodies and methods
JPS60100516A (en) 1983-11-04 1985-06-04 Takeda Chem Ind Ltd Preparation of sustained release microcapsule
CA1256638A (en) 1984-07-06 1989-06-27 Motoaki Tanaka Polymer and its production
US4879224A (en) 1985-01-10 1989-11-07 Biogen, Inc. DNA sequences, recombinant DNA molecules and processes for producing human phospholipase inhibitor polypeptides
JP2551756B2 (en) 1985-05-07 1996-11-06 武田薬品工業株式会社 Polyoxycarboxylic acid ester and method for producing the same
US5756696A (en) 1986-01-16 1998-05-26 Regents Of The University Of California Compositions for chromosome-specific staining
US5447841A (en) 1986-01-16 1995-09-05 The Regents Of The Univ. Of California Methods for chromosome-specific staining
US6280929B1 (en) 1986-01-16 2001-08-28 The Regents Of The University Of California Method of detecting genetic translocations identified with chromosomal abnormalities
US4946778A (en) 1987-09-21 1990-08-07 Genex Corporation Single polypeptide chain binding molecules
EP0317001B1 (en) 1987-11-16 1993-02-03 Janssen Pharmaceutica N.V. A new universally applicable detection system based 0n ultra small colloidal metal particles
DE3811692A1 (en) * 1988-04-07 1989-10-19 Idt Ag Fuer In Vivo Diagnostik DIAGNOSTIC METHOD AND MEANS FOR DIAGNOSIS AND TREATMENT OF IMMUNOLOGICAL DISEASES
CA1308022C (en) * 1988-08-11 1992-09-29 Eleftherios P. Diamandis Multiple fluorescence labelling with europium chelators
DE3836656A1 (en) 1988-10-27 1990-05-03 Boehringer Mannheim Gmbh NEW DIGOXIGENINE DERIVATIVES AND THEIR USE
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5595707A (en) 1990-03-02 1997-01-21 Ventana Medical Systems, Inc. Automated biological reaction apparatus
US5225325A (en) 1990-03-02 1993-07-06 Ventana Medical Systems, Inc. Immunohistochemical staining method and reagents therefor
JPH06506058A (en) * 1991-03-20 1994-07-07 ジーイーシー−マーコニ　リミテッド Separation method
WO1993020094A1 (en) * 1992-03-27 1993-10-14 Abbott Laboratories Haptens, tracers, immunogens and antibodies for quinoline
US6100099A (en) * 1994-09-06 2000-08-08 Abbott Laboratories Test strip having a diagonal array of capture spots
US5403747A (en) 1992-04-01 1995-04-04 Thomas Jefferson University Protein assay using microwave energy
US5350686A (en) 1992-04-16 1994-09-27 Pioneer Hi-Bred International, Inc. Microwave acceleration of enzyme-catalyzed modification of macromolecules
US5834202A (en) 1992-08-04 1998-11-10 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
ES2322324T3 (en) 1992-08-21 2009-06-19 Vrije Universiteit Brussel IMMUMOGLOBULINS DESPROVISTAS OF LIGHT CHAINS.
US6005079A (en) 1992-08-21 1999-12-21 Vrije Universiteit Brussels Immunoglobulins devoid of light chains
US5393891A (en) 1993-06-08 1995-02-28 The Du Pont Merck Pharmaceutical Company Immunoassay reagents and methods for detecting brequinar and analogs
US5679582A (en) 1993-06-21 1997-10-21 Scriptgen Pharmaceuticals, Inc. Screening method for identifying ligands for target proteins
US5661040A (en) 1993-07-13 1997-08-26 Abbott Laboratories Fluorescent polymer labeled conjugates and intermediates
EP0708767B1 (en) * 1993-07-20 2001-02-14 Abbott Laboratories Haptens, tracers, immunogens and antibodies for carbazole and dibenzofuran derivatives
US5731171A (en) 1993-07-23 1998-03-24 Arch Development Corp. Sequence independent amplification of DNA
US5643761A (en) 1993-10-27 1997-07-01 The Trustees Of Columbia University In The City Of New York Method for generating a subtracted cDNA library and uses of the generated library
US5487975A (en) 1993-11-15 1996-01-30 Ventana Medical Systems, Inc. Biotin/avidin formulation
DE4407538C1 (en) * 1994-03-07 1995-02-23 Deutsches Krebsforsch Binding reagent for cell surface protein and effector cell
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
CA2560114A1 (en) 1994-07-15 1996-02-01 The University Of Iowa Research Foundation Immunomodulatory oligonucleotides
US6207646B1 (en) 1994-07-15 2001-03-27 University Of Iowa Research Foundation Immunostimulatory nucleic acid molecules
US6429199B1 (en) 1994-07-15 2002-08-06 University Of Iowa Research Foundation Immunostimulatory nucleic acid molecules for activating dendritic cells
US6239116B1 (en) 1994-07-15 2001-05-29 University Of Iowa Research Foundation Immunostimulatory nucleic acid molecules
GB9420355D0 (en) 1994-10-10 1994-11-23 Univ Nottingham Preparation of protein microspheres, films and coatings
US6057099A (en) 1994-12-02 2000-05-02 Intelligene Ltd. Detection of nucleic acid sequences
US5830645A (en) 1994-12-09 1998-11-03 The Regents Of The University Of California Comparative fluorescence hybridization to nucleic acid arrays
US5648245A (en) 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
US5684142A (en) 1995-06-07 1997-11-04 Oncor, Inc. Modified nucleotides for nucleic acid labeling
US5883081A (en) 1995-07-26 1999-03-16 The Regents Of The University Of California Isolation of novel HIV-2 proviruses
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US6582921B2 (en) 1996-07-29 2003-06-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses thereof
US6750016B2 (en) 1996-07-29 2004-06-15 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6361944B1 (en) 1996-07-29 2002-03-26 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6506564B1 (en) 1996-07-29 2003-01-14 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
JPH10104230A (en) * 1996-09-30 1998-04-24 Aisin Seiki Co Ltd Method of detecting nucleic acid or the like, and labeled substance and detected substance
DE69718054T2 (en) 1996-10-04 2003-07-17 Univ Heidelberg METHOD FOR PURIFYING DNA
US5952890A (en) 1997-02-05 1999-09-14 Fox Enterprises, Inc. Crystal oscillator programmable with frequency-defining parameters
AU738513B2 (en) 1997-02-28 2001-09-20 University Of Iowa Research Foundation, The Use of nucleic acids containing unmethylated CpG dinucleotide in the treatment of LPS-associated disorders
US6406705B1 (en) 1997-03-10 2002-06-18 University Of Iowa Research Foundation Use of nucleic acids containing unmethylated CpG dinucleotide as an adjuvant
US5994071A (en) 1997-04-04 1999-11-30 Albany Medical College Assessment of prostate cancer
CA2301575C (en) 1997-05-20 2003-12-23 Ottawa Civic Hospital Loeb Research Institute Vectors and methods for immunization or therapeutic protocols
JPH1151935A (en) * 1997-08-06 1999-02-26 Aisin Seiki Co Ltd Simultaneous detection method for plural kinds of target molecules
US6124120A (en) 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
US6322901B1 (en) 1997-11-13 2001-11-27 Massachusetts Institute Of Technology Highly luminescent color-selective nano-crystalline materials
JPH11240879A (en) * 1997-12-22 1999-09-07 Nissan Chem Ind Ltd Polyclonal antibody against pyrazolesulfonylurea and its detection
US6358682B1 (en) 1998-01-26 2002-03-19 Ventana Medical Systems, Inc. Method and kit for the prognostication of breast cancer
US6582962B1 (en) 1998-02-27 2003-06-24 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
EP1073892B1 (en) 1998-02-27 2010-11-24 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
US6855559B1 (en) 1998-09-03 2005-02-15 Ventana Medical Systems, Inc. Removal of embedding media from biological samples and cell conditioning on automated staining instruments
US20030211630A1 (en) 1998-02-27 2003-11-13 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
US6235480B1 (en) 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
EP1067956B1 (en) 1998-04-03 2007-03-14 University Of Iowa Research Foundation Methods and products for stimulating the immune system using immunotherapeutic oligonucleotides and cytokines
US7550298B2 (en) 1998-09-03 2009-06-23 Ventana Medical Systems, Inc. Automated immunohistochemical and in situ hybridization assay formulations
US6855552B2 (en) 1998-09-03 2005-02-15 Ventana Medical Systems Automated immunohistochemical and in situ hybridization assay formulations
JP2000095769A (en) * 1998-09-22 2000-04-04 Kankyo Meneki Gijutsu Kenkyusho:Kk Hapten compound of probenazole, antibody and assay
WO2000029617A2 (en) 1998-09-24 2000-05-25 Advanced Research And Technology Institute, Inc. Water-soluble luminescent quantum dots and bioconjugates thereof
US6414133B1 (en) 1998-10-13 2002-07-02 Ventana Medical Systems, Inc. Multiple fusion probes
US7569344B2 (en) 1998-10-26 2009-08-04 Ventana Medical Systems, Inc. Detection of human papilloma virus in papanicolaou (Pap) smears
US6432320B1 (en) 1998-11-02 2002-08-13 Patrick Bonsignore Refrigerant and heat transfer fluid additive
US6699973B1 (en) 1998-11-19 2004-03-02 Elan Corporation, Plc Antibodies to peptides that target GIT receptors and related methods
WO2000041524A2 (en) 1999-01-11 2000-07-20 President And Fellows Of Harvard College Isothermal amplification of dna
WO2000049049A1 (en) 1999-02-19 2000-08-24 Michigan State University An antigen test to detect equine protozoal myeloencephalitis in horse serum and cerebrospinal fluid
US6696304B1 (en) 1999-02-24 2004-02-24 Luminex Corporation Particulate solid phase immobilized protein quantitation
US6544798B1 (en) 1999-02-26 2003-04-08 Ventana Medical Systems, Inc. Removal of embedding media from biological samples and cell conditioning on automated staining instruments
US6673214B1 (en) 1999-04-09 2004-01-06 Rocky Mountain Biosystems, Inc. Energy enhanced reaction catalysis and uses thereof
AU763162B2 (en) 1999-04-13 2003-07-17 Wilex Ag Diagnostic and therapeutic use of antibodies against the urokinase receptor
EP1179185B1 (en) * 1999-05-07 2009-08-12 Life Technologies Corporation A method of detecting an analyte using semiconductor nanocrystals
US6180349B1 (en) 1999-05-18 2001-01-30 The Regents Of The University Of California Quantitative PCR method to enumerate DNA copy number
US6326148B1 (en) 1999-07-12 2001-12-04 The Regents Of The University Of California Detection of copy number changes in colon cancer
DE19933701A1 (en) 1999-07-19 2001-01-25 Wilex Biotechnology Gmbh Cyclic peptidomimetic urokinase receptor antagonists
JP2001089484A (en) * 1999-09-22 2001-04-03 Kankyo Meneki Gijutsu Kenkyusho:Kk Hapten compound of tricyclazole, antibody and assay
WO2001029265A1 (en) * 1999-10-15 2001-04-26 Ventana Medical Systems, Inc. Method of detecting single gene copies in-situ
US20060275784A1 (en) 1999-10-26 2006-12-07 Ventana Medical Systems, Inc. Detection of Human Papilloma Virus in Papanicolaou (Pap) Smears
CA2397417A1 (en) 2000-01-12 2001-07-19 Sarah S. Bacus Method for quantitating a protein by image analysis
JP2004501340A (en) 2000-01-13 2004-01-15 ナノスフェアー　インコーポレイテッド Oligonucleotide-attached nanoparticles and methods of use
WO2001071354A2 (en) 2000-03-20 2001-09-27 Massachusetts Institute Of Technology Inorganic particle conjugates
JP4317913B2 (en) 2000-04-14 2009-08-19 ベンタナ・メデイカル・システムズ・インコーポレーテツド Quantification method of AKT protein expression
US6291187B1 (en) 2000-05-12 2001-09-18 Molecular Staging, Inc. Poly-primed amplification of nucleic acid sequences
US6828097B1 (en) 2000-05-16 2004-12-07 The Childrens Mercy Hospital Single copy genomic hybridization probes and method of generating same
JP2004523201A (en) 2000-05-16 2004-08-05 ザ チルドレンズ マーシー ホスピタル Single copy genome hybridization probe and method for producing the same
US6794362B1 (en) 2000-05-30 2004-09-21 Connective Tissue Imagineering Llc Asparagine containing elastin peptide analogs
US7763421B2 (en) 2000-06-05 2010-07-27 Ventana Medical Systems, Inc. Methods for producing nucleic acid hybridization probes that amplify hybridization signal by promoting network formation
US6323009B1 (en) 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
US6447692B1 (en) 2000-08-04 2002-09-10 Hrl Laboratories, Llc Nanometer sized phase change materials for enhanced heat transfer fluid performance
US6942970B2 (en) 2000-09-14 2005-09-13 Zymed Laboratories, Inc. Identifying subjects suitable for topoisomerase II inhibitor treatment
US20040209303A1 (en) 2000-10-03 2004-10-21 Martin Mark T. Methods and compositions for directed microwave chemistry
US6649138B2 (en) 2000-10-13 2003-11-18 Quantum Dot Corporation Surface-modified semiconductive and metallic nanoparticles having enhanced dispersibility in aqueous media
US6576291B2 (en) 2000-12-08 2003-06-10 Massachusetts Institute Of Technology Preparation of nanocrystallites
AU2904602A (en) 2000-12-11 2002-06-24 Harvard College Nanosensors
US20020083888A1 (en) 2000-12-28 2002-07-04 Zehnder Donald A. Flow synthesis of quantum dot nanocrystals
US20030022166A1 (en) 2001-01-19 2003-01-30 Colin Collins Repeat-free probes for molecular cytogenetics
US6656685B2 (en) 2001-01-29 2003-12-02 Ventana Medical Systems, Inc. Hybridization buffers using low molecular weight dextran sulfate and methods for their use
WO2003004944A2 (en) 2001-01-30 2003-01-16 Materials And Electrochemical Research (Mer) Corporation Nano carbon materials for enhancing thermal transfer in fluids
DE10105912A1 (en) 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Recombinant Proteinase K
US20030003537A1 (en) 2001-04-30 2003-01-02 Ventana Medical Systems, Inc. Method and composition of matter for enhancing staining of microorganisms
JP2005514582A (en) 2001-04-30 2005-05-19 ベンタナ・メデイカル・システムズ・インコーポレーテツド Reagents and methods for automated hybridization
NZ511705A (en) * 2001-05-14 2004-03-26 Horticulture & Food Res Inst Methods and rapid immunoassay device for detecting progesterone and other steroids
US20030022243A1 (en) 2001-06-20 2003-01-30 Les Kondejewski Protein aggregation assays and uses thereof
WO2003005030A1 (en) * 2001-07-02 2003-01-16 Arctic Diagnostics Oy Two-photon absorbing dipyrrometheneboron difluoride dyes and their applications
AU2002367778A1 (en) 2001-07-20 2003-11-10 Quantum Dot Corporation Luminescent nanoparticles and methods for their preparation
US20030066998A1 (en) 2001-08-02 2003-04-10 Lee Howard Wing Hoon Quantum dots of Group IV semiconductor materials
JP2005531490A (en) * 2001-10-09 2005-10-20 ３−ディメンショナル ファーマシューティカルズ， インコーポレイテッド Substituted diphenyloxazoles, their synthesis, and their use as fluorescent probes
US6977148B2 (en) 2001-10-15 2005-12-20 Qiagen Gmbh Multiple displacement amplification
US6617137B2 (en) 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
US20030165485A1 (en) 2001-11-09 2003-09-04 Goran Bertilsson Functional role and potential therapeutic use of Reelin, Gas6 and Protein S in relation to adult neural stem or progenitor cells
WO2003048317A2 (en) 2001-11-30 2003-06-12 Children's Hospital Medical Center Antibodies to magmas and uses thereof
US20050019901A1 (en) 2002-01-31 2005-01-27 Evgenia Matveeva Methods for synthesis of bio-active nanoparticles and nanocapsules for use in optical bio-disc assays and disc assembly including same
US20040248151A1 (en) 2002-04-05 2004-12-09 Ventana Medical Systems, Inc. Method for predicting the response to HER2-directed therapy
US7285289B2 (en) 2002-04-12 2007-10-23 Nagy Jon O Nanoparticle vaccines
US20040057958A1 (en) 2002-05-17 2004-03-25 Waggoner David W. Immunogenicity-enhancing carriers and compositions thereof and methods of using the same
US7476442B2 (en) 2002-07-17 2009-01-13 Massachusetts Institute Of Technology Nanoparticle chains and preparation thereof
AU2003302133A1 (en) 2002-10-25 2004-06-15 Sense Proteomic Limited Uses of ble proteins and antibiotics from the bleomycin family
US7200252B2 (en) 2002-10-28 2007-04-03 Ventana Medical Systems, Inc. Color space transformations for use in identifying objects of interest in biological specimens
US7233817B2 (en) 2002-11-01 2007-06-19 Brian Yen Apparatus and method for pattern delivery of radiation and biological characteristic analysis
US20040101822A1 (en) 2002-11-26 2004-05-27 Ulrich Wiesner Fluorescent silica-based nanoparticles
GB0227738D0 (en) 2002-11-28 2003-01-08 Univ Liverpool Nanoparticle conjugates and method of production thereof
US20040115727A1 (en) 2002-12-11 2004-06-17 Allergan, Inc., A Corporation Evolved clostridial toxins with altered protease specificity
US7056471B1 (en) 2002-12-16 2006-06-06 Agency For Science Technology & Research Ternary and quarternary nanocrystals, processes for their production and uses thereof
WO2004094652A2 (en) 2003-04-22 2004-11-04 Neurobiotex, Inc. Zinc-based screening test and kit for early diagnosis of prostate cancer
US6944333B2 (en) 2003-04-30 2005-09-13 Ventana Medical Systems, Inc. Color image compression via spectral decorrelation and elimination of spatial redundancy
EP2530719B1 (en) 2003-05-07 2020-08-05 Indiana University Research and Technology Corporation Alloyed semiconductor concentration-gradient quantum dots, use and method of fabricating thereof
AU2004241591A1 (en) 2003-05-19 2004-12-02 Ventana Medical Systems, Inc. Electrophoretic in situ tissue staining
US20050181394A1 (en) 2003-06-20 2005-08-18 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
ATE437369T1 (en) 2003-06-24 2009-08-15 Ventana Med Syst Inc ENZYME-CATALYzed METAL DEPOSITION FOR ENHANCED IN-SITU DETECTION OF IMMUNOHISTOLOGICAL EPITOPES AND NUCLEIC ACID SEQUENCES
KR100657891B1 (en) 2003-07-19 2006-12-14 삼성전자주식회사 Semiconductor nanocrystal and method for preparing the same
US20050048498A1 (en) 2003-08-29 2005-03-03 Applera Corporation Compositions, methods, and kits for assembling probes
KR100571817B1 (en) 2003-09-19 2006-04-17 삼성전자주식회사 A method for detecting a target nucleic acid by using a detection probe capable of hybridizing with a tag sequence
WO2005030929A2 (en) 2003-09-23 2005-04-07 Atom Sciences, Inc. Polymeric nucleic acid hybridization probes
BRPI0416603A (en) 2003-11-07 2007-01-30 Immunex Corp antibody that binds to the human interleukin-4 (il-4) receptor
TW200526224A (en) 2003-12-22 2005-08-16 Alcon Inc Short form c-Maf transcription factor antagonists for treatment of glaucoma
US7541455B2 (en) 2003-12-22 2009-06-02 Ventana Medical Systems, Inc. Microwave mediated synthesis of nucleic acid probes
US7104313B2 (en) 2003-12-31 2006-09-12 Intel Corporation Apparatus for using fluid laden with nanoparticles for application in electronic cooling
US7244665B2 (en) 2004-04-29 2007-07-17 Micron Technology, Inc. Wafer edge ring structures and methods of formation
JP2007535944A (en) 2004-05-04 2007-12-13 ヴェンタナ メディカル システムズ インコーポレイテッド Internal control for in situ hybridization
US20060110744A1 (en) 2004-11-23 2006-05-25 Sampas Nicolas M Probe design methods and microarrays for comparative genomic hybridization and location analysis
US7947839B2 (en) * 2004-12-01 2011-05-24 Genentech, Inc. Heterocyclic-substituted bis-1,8 naphthalimide compounds, antibody drug conjugates, and methods of use
US20060160116A1 (en) 2004-12-16 2006-07-20 The Regents Of The University Of California Repetitive sequence-free DNA libraries
US20060246524A1 (en) 2005-04-28 2006-11-02 Christina Bauer Nanoparticle conjugates
US7615800B2 (en) 2005-09-14 2009-11-10 Eastman Kodak Company Quantum dot light emitting layer
ES2548518T3 (en) * 2005-11-23 2015-10-19 Ventana Medical Systems, Inc. Molecular conjugate
DE602006011959D1 (en) 2005-12-30 2010-03-11 Ventana Med Syst Inc NA +, K + -ATPASE EXPRESSION IN ZERVIX DYSPLASIA AND CANCER
EP2069537B1 (en) 2006-09-01 2011-08-31 Ventana Medical Systems, Inc. Method for producing nucleic acid probes
WO2008063378A2 (en) 2006-11-01 2008-05-29 Ventana Medical Systems, Inc. Haptens, hapten conjugates, compositions thereof and method for their preparation and use
US7682789B2 (en) 2007-05-04 2010-03-23 Ventana Medical Systems, Inc. Method for quantifying biomolecules conjugated to a nanoparticle
EP2167963B1 (en) * 2007-05-23 2019-04-17 Ventana Medical Systems, Inc. Polymeric carriers for immunohistochemistry and in situ hybridization
EP2406234A1 (en) * 2009-03-12 2012-01-18 GlaxoSmithKline LLC Thiazole sulfonamide and oxazole sulfonamide kinase inhibitors

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9180215B2 (en) 2007-02-07 2015-11-10 Purdue Research Foundation Positron emission tomography imaging method

Also Published As

Publication number Publication date
JP6691092B2 (en) 2020-04-28
US8618265B2 (en) 2013-12-31
JP6259477B2 (en) 2018-01-10
US11092594B2 (en) 2021-08-17
EP2078197B1 (en) 2016-03-23
US20100184087A1 (en) 2010-07-22
EP2444806B1 (en) 2014-05-28
CA2858359A1 (en) 2008-05-29
DK3029461T3 (en) 2018-02-05
JP2015072275A (en) 2015-04-16
ES2663080T3 (en) 2018-04-11
AU2007322261B2 (en) 2014-01-23
EP2442107A3 (en) 2012-11-14
JP7000489B2 (en) 2022-01-19
US8846320B2 (en) 2014-09-30
JP2018040813A (en) 2018-03-15
JP2016136150A (en) 2016-07-28
WO2008063378A3 (en) 2008-12-04
ES2465465T3 (en) 2014-06-05
ES2493166T3 (en) 2014-09-11
CA2995467C (en) 2020-03-10
EP2444807A3 (en) 2013-02-27
CA3069091C (en) 2021-09-14
DK3276349T3 (en) 2019-10-28
JP5576120B2 (en) 2014-08-20
DK2442107T3 (en) 2014-05-19
CA2858359C (en) 2018-04-03
CA2995467A1 (en) 2008-05-29
US20080268462A1 (en) 2008-10-30
CA3069091A1 (en) 2008-05-29
EP2442107A2 (en) 2012-04-18
JP5973524B2 (en) 2016-08-23
US20140323336A1 (en) 2014-10-30
ES2491743T3 (en) 2014-09-08
DK2444807T3 (en) 2014-08-11
CA2666234A1 (en) 2008-05-29
EP2078197A2 (en) 2009-07-15
JP2020126057A (en) 2020-08-20
EP2444807A2 (en) 2012-04-25
EP3564674A1 (en) 2019-11-06
EP3029461A1 (en) 2016-06-08
WO2008063378A2 (en) 2008-05-29
EP2442107B1 (en) 2014-03-12
EP2444806A2 (en) 2012-04-25
CA3125026C (en) 2023-04-04
EP3276349B1 (en) 2019-08-07
EP3564674B1 (en) 2021-08-25
DK2444806T3 (en) 2014-07-21
ES2905054T3 (en) 2022-04-06
EP2078197A4 (en) 2010-03-24
ES2761949T3 (en) 2020-05-21
EP3029461B1 (en) 2017-12-20
US9719986B2 (en) 2017-08-01
ES2570600T3 (en) 2016-05-19
EP2444807B1 (en) 2014-06-11
JP2010508532A (en) 2010-03-18
AU2007322261A1 (en) 2008-05-29
EP2444806A3 (en) 2013-02-27
US7695929B2 (en) 2010-04-13
US20180120306A1 (en) 2018-05-03
JP2013231044A (en) 2013-11-14
WO2008063378A9 (en) 2008-07-31
EP3276349A1 (en) 2018-01-31
US20100297725A1 (en) 2010-11-25
DK2078197T3 (en) 2016-05-23
DK3564674T3 (en) 2021-10-18
CA3125026A1 (en) 2008-05-29
JP5722952B2 (en) 2015-05-27

Similar Documents

Publication Publication Date Title
CA2666234C (en) Haptens, hapten conjugates, compositions thereof and method for their preparation and use
WO2012003476A3 (en) Hapten conjugates for target detection
WO2007061793A3 (en) Modified cardiolipin and uses therefor
WO2007035864A3 (en) Electrophoresis standards, methods and kits
WO2008135862A3 (en) Devices and methods for detecting analytes
EP1905846A3 (en) Markers of renal transplant rejection and renal damage
WO2007123880A3 (en) Methods and kits for early stage caries detection
WO2012071428A3 (en) Methods and/or use of oligonucleotide conjugates for assays and detections
WO2007135368A3 (en) Dye compounds and the use of their labelled conjugates
WO2008005464A3 (en) Quantifiable internal reference standards for immunohistochemistry and uses thereof
WO2008020823A3 (en) Cooperative reporter systems, components, and methods for analyte detection
WO2009024805A8 (en) An enzyme detection device
WO2008112008A3 (en) Sensor proteins and assay methods
WO2007143074A3 (en) Boosted charge transfer pipeline
WO2007132120A3 (en) Procedure and methods for detecting alzheimer's disease
WO2008103319A3 (en) Compounds and methods for use in detecting gabapentin
WO2009079212A3 (en) Linked peptide fluorogenic biosensors
WO2007027796A3 (en) Proximity ligation assays with peptide conjugate 'burrs' and aptamers for the sensitive detection of spores and cancer cells
WO2009025364A1 (en) Non-specific reaction inhibitor
WO2007104314A3 (en) INHIBITION OF GASCl
WO2006099537A3 (en) The use of antibody-surrogate antigen systems for detection of analytes
WO2007051603A3 (en) Kit comprising 2, 4- dichlorophenoxyacetic acd derivatives and antibodies for highly sensitive detection assays
WO2009117726A3 (en) High-sensitive fluorescent energy transfer assay using fluorescent amino acids and fluoresent proteins
WO2007110756A3 (en) Assay device and method with magnetic field and time-varying signals
WO2009031484A1 (en) Sheet for developing liquid

Legal Events

Date Code Title Description
EEER Examination request