CA2711007A1 - G-csf analog compositions and methods - Google Patents

G-csf analog compositions and methods Download PDF

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Publication number
CA2711007A1
CA2711007A1 CA2711007A CA2711007A CA2711007A1 CA 2711007 A1 CA2711007 A1 CA 2711007A1 CA 2711007 A CA2711007 A CA 2711007A CA 2711007 A CA2711007 A CA 2711007A CA 2711007 A1 CA2711007 A1 CA 2711007A1
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Prior art keywords
csf
molecule
analogs
methods
csf molecule
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CA2711007A
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French (fr)
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CA2711007C (en
Inventor
Timothy D. Osslund
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Amgen Inc
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Amgen Inc.
Timothy D. Osslund
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Application filed by Amgen Inc., Timothy D. Osslund filed Critical Amgen Inc.
Publication of CA2711007A1 publication Critical patent/CA2711007A1/en
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Publication of CA2711007C publication Critical patent/CA2711007C/en
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00Drugs for disorders of the urinary system
    • A61P13/12Drugs for disorders of the urinary system of the kidneys
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00Drugs for dermatological disorders
    • A61P17/02Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00Drugs for skeletal disorders
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04Antibacterial agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/10Antimycotics
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • A61P35/02Antineoplastic agents specific for leukemia
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00Drugs for immunological or allergic disorders
    • A61P37/02Immunomodulators
    • A61P37/04Immunostimulants
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00Drugs for disorders of the blood or the extracellular fluid
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00Drugs for disorders of the blood or the extracellular fluid
    • A61P7/06Antianaemics
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00Drugs for disorders of the cardiovascular system
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52Cytokines; Lymphokines; Interferons
    • C07K14/53Colony-stimulating factor [CSF]
    • C07K14/535Granulocyte CSF; Granulocyte-macrophage CSF
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2299/00Coordinates from 3D structures of peptides, e.g. proteins or enzymes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A90/00Technologies having an indirect contribution to adaptation to climate change
    • Y02A90/10Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation

Abstract

Provided herein are granulocyte colony stimulating factor ("G-CSF") analogs, compositions containing such analogs, and related compositions. In another aspect, provided herein are nucleic acids encoding the present analogs or related nucleic acids, related host cells and vectors. In yet another aspect, provided herein are computer programs and apparatuses for expressing the three dimensional structure of G-CSF and analogs thereof. In another aspect, provided herein are methods for rationally designing G-CSF analogs and related compositions. In yet another aspect, provided herein are methods for treatment using the present G-CSF analogs.

Claims (5)

1. A G-CSF molecule comprising amino acids 2-175 as set forth in SEQ ID
NO: 1, having the overall three-dimensional conformation as set forth in Figure 4, wherein the three-dimensional structure of the CD loop is altered from that represented in a computer program displaying the x-ray crystallographic coordinates as set forth in Figure 5.
2. The G-CSF molecule of claim 1, additionally comprising an N-terminal methionine at position 1.
3. The G-CSF molecule of claim 1 or 2, wherein the overall three-dimensional conformation of the G-CSF molecule having an altered three-dimensional structure of the CD loop is:
a) an anti-parallel 4-alpha helical bundle with a left hand twist;
b) overall dimensions of 45 .ANG. x 30 x .ANG. x 24 .ANG.; and c) helix crossing angles ranging from -167.5° to 159.4°.
4. A pharmaceutical composition comprising a G-CSF molecule according to claim 1, 2 or 3, and a pharmaceutically acceptable carrier.
5. The G-CSF molecule of claim 1 or 2, wherein said molecule is a hybrid molecule of G-CSF and another protein.
CA2711007A 1993-01-28 1994-01-25 G-csf analog compositions and methods Expired - Lifetime CA2711007C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/010,099 1993-01-28
US08/010,099 US5581476A (en) 1993-01-28 1993-01-28 Computer-based methods and articles of manufacture for preparing G-CSF analogs
CA2381489A CA2381489C (en) 1993-01-28 1994-01-25 G-csf analog compositions and methods

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA2381489A Division CA2381489C (en) 1993-01-28 1994-01-25 G-csf analog compositions and methods

Publications (2)

Publication Number Publication Date
CA2711007A1 true CA2711007A1 (en) 1994-08-04
CA2711007C CA2711007C (en) 2012-10-02

Family

ID=21743861

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CA002153896A Expired - Lifetime CA2153896C (en) 1993-01-28 1994-01-25 G-csf analog compositions and methods
CA002239360A Expired - Lifetime CA2239360C (en) 1993-01-28 1994-01-25 G-csf analog compositions and methods
CA2711007A Expired - Lifetime CA2711007C (en) 1993-01-28 1994-01-25 G-csf analog compositions and methods
CA2381489A Expired - Lifetime CA2381489C (en) 1993-01-28 1994-01-25 G-csf analog compositions and methods

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CA002153896A Expired - Lifetime CA2153896C (en) 1993-01-28 1994-01-25 G-csf analog compositions and methods
CA002239360A Expired - Lifetime CA2239360C (en) 1993-01-28 1994-01-25 G-csf analog compositions and methods

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Country Status (22)

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US (6) US5581476A (en)
EP (8) EP0890640A3 (en)
JP (7) JPH08506018A (en)
KR (2) KR100375274B1 (en)
CN (3) CN1970571B (en)
AT (2) ATE195552T1 (en)
AU (2) AU697451B2 (en)
CA (4) CA2153896C (en)
DE (1) DE69425516T2 (en)
DK (3) DK1482046T3 (en)
ES (3) ES2150951T3 (en)
FI (2) FI120769B (en)
GR (1) GR3034745T3 (en)
HK (2) HK1009288A1 (en)
IL (6) IL108446A (en)
LU (1) LU92265I2 (en)
LV (1) LV12642B (en)
NO (3) NO952974L (en)
NZ (5) NZ332188A (en)
PT (3) PT612846E (en)
WO (1) WO1994017185A1 (en)
ZA (1) ZA94568B (en)

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