CN101387630B - Method for predicting shelf life of UHT milk - Google Patents
Method for predicting shelf life of UHT milk Download PDFInfo
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- CN101387630B CN101387630B CN2008101716988A CN200810171698A CN101387630B CN 101387630 B CN101387630 B CN 101387630B CN 2008101716988 A CN2008101716988 A CN 2008101716988A CN 200810171698 A CN200810171698 A CN 200810171698A CN 101387630 B CN101387630 B CN 101387630B
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Abstract
The invention relates to a method for predicating UHT milk shelf life, comprising the steps of: setting an upper lipid content value of UHT milk under a normal temperature T2 as a shelf life reference value, while the storage time and the storage temperature under the shelf life reference value meet an equation: Logt=A/T+M, wherein t means the storage time, T means the storage temperature, A means the characteristic coefficient, M means the correlation coefficient; checking the upper lipid content of UHT milk stored under an actual storage temperature T1, substituting the actual storage temperature T1 and the actual storage time t1 into the equation when the upper lipid particle diameter reaches the shelf life reference value in the actual storage time t1, to calculate A; substituting M, A and T2 into the equation, to calculate the shelf life t2 of the UHT milk under normal temperature. The invention builds a method for using upper lipid content to predicate UHT milk shelf life, which can find the shelf life of a new developed UHT product in short time, to convert developmental achievement into economic benefit quickly.
Description
Technical field
The present invention relates to a kind of method of predicating UHT milk shelf life, especially a kind of method of utilizing the upper strata fat content that detects the UHT Ruzhong to come predicating UHT milk shelf life.
Background technology
UHT (Ultra High Temperature) breast is a kind of product that occurs in the sixties in 20th century, and it is meant the dairy product that the technological process that adopts ultra high temperature sterilization (UHTS) and sterile filling processes.
Compare with Pasteur's breast, the UHT breast has solved the problem of liquid newborn transportation, storage, fresh-keeping difficulty, has kept nutriments such as protein, calcium effectively.Its characteristic is: product can reach commercial sterilization, only needs to preserve at normal temperatures, and distribution does not need cold chain, shelf life long (generally can store 1～6 month), and the nutrition in Ruzhong (except indivedual water soluble vitamins) is identical with pasteurization milk substantially.UHT compares with traditional pasteurize, can significantly reduce the loss of cow's milk color, local flavor and nutritive value, thereby becomes present the best and processing mode with fastest developing speed.
The time that food shelf index futures product can be stored, product quality can be accepted and satisfy the time span of client's quality requirements in period of storage.Shelf life accelerated tests (ASLT, Accelerated shelf-lifetesting) mainly is at the product of long shelf life long inefficient problem of cycle and a kind of experimental technique of growing up under study for action.This experimental technique is product to be placed quicken its reaction under the fierce condition, makes the real shelf life that (is shorter than the time of its real shelf life) in the short period of time and dopes product.The condition that accelerated test is used mainly contains: high temperature, high humidity, illumination, stricter temperature cycles and high concussion etc., use high humidity, high temperature and illumination in food service industry more, wherein the temperature accelerated test use comparatively extensive.
In recent years, businessman is devoted to study the product of long shelf life in order to obtain to sell interests, for example is subjected to shelf life extension (Extended shelf-life ESL) breast, i.e. the ESL breast of domestic and international extensive concern in recent years.The product of research long shelf life has become a kind of development trend.
At present, all do not detect the particularly method of UHT milk shelf life of cow's milk in GB and the industry standard, present judgement for UHT new product shelf life, the main factors such as the comprehensive sterilization mode of research and development person, packaged form that rely on, provide empirical value, afterwards product is placed under normal temperature condition, routine observation is determined the shelf life of product with this.The result that this method is judged has very strong subjectivity, and judges chronicly, needs to verify over 6-8 month the accuracy of judgement usually.The time that research needs in the process of improving the UHT breast and developing new product is long, and drain on manpower and material resources is bigger.
The butter oil density in Ruzhong is less, and according to Stokes (Stokes) law, fat can the nature come-up under hydrodynamic action.The UHT breast is after handling through homogeneous, the granularity of butter oil ball is refine to a certain degree, and the form with colloidal sol is dispersed in the Ruzhong, keeps spacing because of having like charges between the fat globule, and therefore butter oil should comparatively fast not float in the product shelf life.But as things go, the fat floating phenomenon is very common in the UHT Ruzhong, and the UHT breast that has occurs after storing 3 months, and what have will occur in storing 2 weeks even shorter time.
The enzyme in Ruzhong is relevant with the fat floating phenomenon.In the Ruzhong, stable on heating proteinase can hydrolyzing alpha-CN and β-CN, destroys butter oil ball and casein surface structure, causes that fat combine also gathering with fat, fat with casein, form little thin slice and float on newborn top; And lipase has destroyed the structure of fat globule by the hydrolysing milk fat fat, and fat globule is assembled, and fat content increases, and makes the butter oil come-up, influences product quality.
Summary of the invention
The purpose of this invention is to provide a kind of method,, but judge the shelf life of UHT breast within a short period of time by this method with upper strata fat content predicating UHT milk shelf life.
The invention provides a kind of method of predicating UHT milk shelf life, comprise the steps:
1) the upper strata fat content value of setting UHT Ruzhong under the normal temperature T2 is as the shelf life reference value, and period of storage and storage temperature satisfy following equation under the described shelf life reference value:
Logt＝A/T+M
Wherein, t is a period of storage, and T is a storage temperature, and A is a characteristic coefficient, and M is a related coefficient;
2) detect the actual storage temperature T1 upper strata fat content in the UHT Ruzhong of storage down, when actual period of storage t1 detects when obtaining upper strata fat content value and reaching described shelf life reference value, with described actual storage temperature T1 and the above-mentioned equation of described actual period of storage t1 substitution, try to achieve characteristic coefficient A;
3) with described related coefficient M, characteristic coefficient A and the above-mentioned equation of described normal temperature temperature T 2 substitutions, try to achieve described UHT breast shelf life t2 at normal temperatures.
Among the present invention, described shelf life reference value is 6.54%-6.78%.Related coefficient M is 0.035-0.045.
Above-mentioned actual storage temperature T1 is 40-50 ℃, is preferably 45 ℃.Above-mentioned normal temperature T2 is 25 ℃.
Above-mentioned steps 2) in, the upper strata fat content in the UHT Ruzhong that detection in every 3-5 days is once stored.Adopt the upper strata fat content in the UHT Ruzhong of the described storage of milk composition analysis-e/or determining.During detection the packing of the UHT breast stored is carefully cut off, carefully drawn upper strata breast sample 50mL, sample detection with transfer pipet.
At the cow's milk lay up period, fat can constantly be assembled, and the upper strata fat content can constantly increase, and among the present invention, the terminal point of described upper strata fat content is 6.54%-6.78%.Store the UHT breast under 40-50 ℃ of two temperature conditions, when its upper strata fat content reached 6.54%-6.78%, record period of storage t1, storage temperature T1 set related coefficient M.Only need t1, T1 and M substitution Logt=A/T+M can be obtained the UHT breast shelf life of being stored at normal temperatures.
The present invention detects the UHT milk shelf life with the upper strata fat content, when exploitation UHT breast new product, predicts its shelf life in this way within a short period of time, reduces the input of R﹠D cycle and manpower and materials, makes the research and development achievement be converted into economic benefit as early as possible.Method of the present invention can also be used to detecting the shelf life of existing procucts.Method detection speed of the present invention is fast, needs to finish usually in 10-20 days, and generally needs 2-6 month with existing method.Method error of the present invention was less than 10 days, and computing method are simple.
Embodiment
Embodiments of the present invention are intended to explain invention; rather than restriction protection scope of the present invention; the any those of ordinary skill in this area all should be able to know clearly and understand; to specifications disclosed proportional range be can completely inerrably implement of the present invention; any difference slightly on the numerical value that causes because of the difference in cow's milk breast source should be as the different slightly reason of testing result of the present invention; so the present invention is intended to protect the method for coming predicating UHT milk shelf life of utilizing the upper strata fat content that detects the UHT Ruzhong, rather than ratio especially strictly will be limited on the some concrete numerical value.
Embodiment 1: the shelf life of prediction UHT breast
The shelf life reference value of setting the upper strata fat content in UHT Ruzhong under the normal temperature (T2=25 ℃) is 6.54%-6.78%, and under this shelf life reference value, period of storage and storage temperature satisfy following equation:
Logt＝A/T+M
Wherein, t is a period of storage, and T is a storage temperature, and A is a characteristic coefficient, and M is a related coefficient.
Related coefficient under this reference value is got M=0.039.
From producing, extract one batch UHT breast sample, be stored under 45 ℃ the content of the upper strata fat of the per five days once actual storage temperature of mensuration (T1=45 ℃) storage UHT breasts.
The detection step of upper strata fat is as follows:
1. carefully cut off packing, carefully draw upper strata breast sample 50mL with transfer pipet.
2. the upper solution of drawing is put into beaker, detect reading of data with milk composition analyser (Ftir DeltaInstruments).
When the upper strata fat content in the UHT Ruzhong of T1=45 ℃ of storage reaches 6.54%, fallen in the shelf life reference value 6.54%-6.78% scope that sets, writing down actual period of storage t1 is 13 days.
With M, T1 and the above-mentioned equation of t1 substitution: i.e. Log13=A/45+0.039, try to achieve characteristic coefficient A=48.37.With M, A and 2=25 ℃ of above-mentioned equation of substitution of normal temperature temperature T, i.e. Logt2=48.37/25+0.039, trying to achieve the shelf life t2 that stores this UHT breast under the normal temperature is 94 days.
This UHT breast is stored down 25 ℃ of normal temperature, detected the upper strata fat content in per five days, the period of storage when the upper strata fat content reaches above-mentioned shelf life reference value under the record normal temperature is 101 days.The actual shelf life of this UHT breast and above-mentioned predicting the outcome differ 7 days.
Embodiment 2: the shelf life of prediction UHT breast
The shelf life reference value of setting the upper strata fat content in UHT Ruzhong under the normal temperature (T2=25 ℃) is 6.54%-6.78%, and under this shelf life reference value, period of storage and storage temperature satisfy following equation:
Logt＝A/T+M
Wherein, t is a period of storage, and T is a storage temperature, and A is a characteristic coefficient, and M is a related coefficient.
Related coefficient under this reference value is got M=0.040.
From producing, extract one batch UHT breast sample, be stored under 45 ℃ the content of the upper strata fat of the per three days once actual storage temperature of mensuration (T1=45 ℃) storage UHT breasts.
The detection step of upper strata fat is identical with detection step among the embodiment 1.
When the upper strata fat content in the UHT Ruzhong of T1=45 ℃ of storage reaches 6.54%, fallen in the shelf life reference value 6.54%-6.78% scope that sets, writing down actual period of storage t1 is 17 days.
With M, T1 and the above-mentioned equation of t1 substitution: i.e. Log17=A/45+0.040, try to achieve characteristic coefficient A=53.57.With M, A and 2=25 ℃ of above-mentioned equation of substitution of normal temperature temperature T, i.e. Logt2=53.57/25+0.040, trying to achieve the shelf life t2 that stores this UHT breast under the normal temperature is 152 days.
This UHT breast is stored down 25 ℃ of normal temperature, detected the upper strata fat content in per three days, the period of storage when the upper strata fat content reaches above-mentioned shelf life reference value under the record normal temperature is 147 days.The actual shelf life of this UHT breast and above-mentioned predicting the outcome differ 5 days.
The shelf life of embodiment 3 prediction UHT breasts
The shelf life reference value of setting the upper strata fat content in UHT Ruzhong under the normal temperature (T2=25 ℃) is 6.54%-6.78%, and under this shelf life reference value, period of storage and storage temperature satisfy following equation:
Logt＝A/T+M
Wherein, t is a period of storage, and T is a storage temperature, and A is a characteristic coefficient, and M is a related coefficient.
Related coefficient under this reference value is got M=0.035.
From producing, extract one batch UHT breast sample, be stored under 45 ℃ the content of the upper strata fat of the per five days once actual storage temperature of mensuration (T1=45 ℃) storage UHT breasts.
The detection step of upper strata fat is identical with detection step among the embodiment 1.
When the upper strata fat content in the UHT Ruzhong of T1=45 ℃ of storage reaches 6.57%, fallen in the shelf life reference value 6.54%-6.78% scope that sets, writing down actual period of storage t1 is 15 days.
With M, T1 and the above-mentioned equation of t1 substitution: i.e. Log15=A/45+0.035, try to achieve characteristic coefficient A=51.35.With M, A and 2=25 ℃ of above-mentioned equation of substitution of normal temperature temperature T, i.e. Logt2=51.35/25+0.035, trying to achieve the shelf life t2 that stores this UHT breast under the normal temperature is 123 days.
This UHT breast is stored down 25 ℃ of normal temperature, detected the upper strata fat content in per five days, the period of storage when the upper strata fat content reaches above-mentioned shelf life reference value under the record normal temperature is 127 days.The actual shelf life of this UHT breast and above-mentioned predicting the outcome differ 4 days.
Embodiment 4 surveys the shelf life of UHT breast
The shelf life reference value of setting the upper strata fat content in UHT Ruzhong under the normal temperature (T2=25 ℃) is 6.54%-6.78%, and under this shelf life reference value, period of storage and storage temperature satisfy following equation:
Logt＝A/T+M
Wherein, t is a period of storage, and T is a storage temperature, and A is a characteristic coefficient, and M is a related coefficient.
Related coefficient under this reference value is got M=0.045.
From producing, extract one batch UHT breast sample, be stored under 45 ℃ the content of the upper strata fat of the per five days once actual storage temperature of mensuration (T1=45 ℃) storage UHT breasts.
The detection step of upper strata fat is identical with detection step among the embodiment 1.
When the upper strata fat content in the UHT Ruzhong of T1=45 ℃ of storage reaches 6.71%, fallen in the shelf life reference value 6.54%-6.78% scope that sets, writing down actual period of storage t1 is 19 days.
With M, T1 and the above-mentioned equation of t1 substitution: i.e. Log19=A/45+0.045, try to achieve characteristic coefficient A=55.52.With M, A and 2=25 ℃ of above-mentioned equation of substitution of normal temperature temperature T, i.e. Logt2=55.52/25+0.045, trying to achieve the shelf life t2 that stores this UHT breast under the normal temperature is 184 days.
This UHT breast is stored down 25 ℃ of normal temperature, detected the upper strata fat content in per five days, the period of storage when the upper strata fat content reaches above-mentioned shelf life reference value under the record normal temperature is 188 days.The actual shelf life of this UHT breast and above-mentioned predicting the outcome differ 4 days.
The shelf life of embodiment 5 prediction UHT breasts
The shelf life reference value of setting the upper strata fat content in UHT Ruzhong under the normal temperature (T2=25 ℃) is 6.54%-6.78%, and under this shelf life reference value, period of storage and storage temperature satisfy following equation:
Logt＝A/T+M
Wherein, t is a period of storage, and T is a storage temperature, and A is a characteristic coefficient, and M is a related coefficient.
Related coefficient under this reference value is got M=0.037.
From producing, extract one batch UHT breast sample, be stored under 40 ℃ the content of the upper strata fat of the per three days once actual storage temperature of mensuration (T1=45 ℃) storage UHT breasts.
The detection step of upper strata fat is identical with detection step among the embodiment 1.
When the upper strata fat content in the UHT Ruzhong of T1=40 ℃ of storage reaches 6.59%, fallen in the shelf life reference value 6.54%-6.78% scope that sets, writing down actual period of storage t1 is 14 days.
With M, T1 and the above-mentioned equation of t1 substitution: i.e. Log14=A/40+0.037, try to achieve characteristic coefficient A=44.37.With M, A and 2=25 ℃ of above-mentioned equation of substitution of normal temperature temperature T, i.e. Logt2=44.37/25+0.037, trying to achieve the shelf life t2 that stores this UHT breast under the normal temperature is 65 days.
This UHT breast is stored down 25 ℃ of normal temperature, detected the upper strata fat content in per three days, the period of storage when the upper strata fat content reaches above-mentioned shelf life reference value under the record normal temperature is 73 days.The actual shelf life of this UHT breast and above-mentioned predicting the outcome differ 8 days.
The shelf life of embodiment 6 prediction UHT breasts
The shelf life reference value of setting the upper strata fat content in UHT Ruzhong under the normal temperature (T2=25 ℃) is 6.54%-6.78%, and under this shelf life reference value, period of storage and storage temperature satisfy following equation:
Logt＝A/T+M
Wherein, t is a period of storage, and T is a storage temperature, and A is a characteristic coefficient, and M is a related coefficient.
Related coefficient under this reference value is got M=0.042.
From producing, extract one batch UHT breast sample, be stored under 50 ℃ the content of the upper strata fat of the per three days once actual storage temperature of mensuration (T1=50 ℃) storage UHT breasts.
The detection step of upper strata fat is identical with detection step among the embodiment 1.
When the upper strata fat content in the UHT Ruzhong of T1=50 ℃ of storage reaches 6.66%, fallen in the shelf life reference value 6.54%-6.78% scope that sets, writing down actual period of storage t1 is 11 days.
With M, T1 and the above-mentioned equation of t1 substitution: i.e. Log15=A/45+0.035, try to achieve characteristic coefficient A=49.97.With M, A and 2=25 ℃ of above-mentioned equation of substitution of normal temperature temperature T, i.e. Logt2=49.975/25+0.042, trying to achieve the shelf life t2 that stores this UHT breast under the normal temperature is 110 days.
This UHT breast is stored down 25 ℃ of normal temperature, detected the upper strata fat content in per three days, the period of storage when the upper strata fat content reaches above-mentioned shelf life reference value under the record normal temperature is 111 days.The actual shelf life of this UHT breast and above-mentioned predicting the outcome differ 1 day.
It should be appreciated by those skilled in the art that per three days described in the above embodiment or per five days detect once, and carrying out according to this time interval of not meaning that whole detections all must strictness.When detecting resulting upper strata fat content comparatively near the shelf life reference value, assay intervals can shorten, and for example detects once every day, to obtain result accurately.
Detect the method for UHT milk shelf life with the upper strata fat content, but determine UHT breast new product shelf life within a short period of time, reduce the input of R﹠D cycle and manpower and materials, make the research and development achievement be converted into economic benefit as early as possible, computing method are simple.
Claims (5)
1. the method for a predicating UHT milk shelf life comprises the steps:
1) the upper strata fat content value of setting UHT Ruzhong under the normal temperature T2 is as the shelf life reference value, and described shelf life reference value is 6.54%-6.78%, and period of storage and storage temperature satisfy following equation under the described shelf life reference value:
Logt＝A/T+M
Wherein, t is a period of storage, and unit is day, and T is a storage temperature, and unit is ℃, and A is a characteristic coefficient, and M is a related coefficient, and described related coefficient M is 0.035-0.045;
2) detect the actual storage temperature T1 upper strata fat content in the UHT Ruzhong of storage down, described actual storage temperature T1 is 40-50 ℃, when actual period of storage t1 detects when obtaining upper strata fat content value and reaching described shelf life reference value, with described actual storage temperature T1 and the above-mentioned equation of described actual period of storage t1 substitution, try to achieve characteristic coefficient A;
3) with described related coefficient M, characteristic coefficient A and the above-mentioned equation of described normal temperature temperature T 2 substitutions, try to achieve described UHT breast shelf life t2 at normal temperatures.
2. the method for claim 1 is characterized in that, described actual storage temperature T1 is 45 ℃.
3. the method for claim 1 is characterized in that, described normal temperature T2 is 25 ℃.
4. the method for claim 1 is characterized in that, described step 2) in, the upper strata fat content in the UHT Ruzhong that detection in every 3-5 days is once stored.
5. as the described method of one of claim 1-4, it is characterized in that, use the upper strata fat content in the UHT Ruzhong of the described storage of milk composition analysis-e/or determining.
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102207460B (en) * | 2010-03-30 | 2012-10-03 | 光明乳业股份有限公司 | Method for detecting shelf life of ultra heat treated milk |
| CN101846666A (en) * | 2010-05-07 | 2010-09-29 | 内蒙古伊利实业集团股份有限公司 | Method for calculating shelf life of UHT pure milk and method for calculating content of upper protein of UHT pure milk |
| CN105558007B (en) * | 2016-01-18 | 2019-09-27 | 内蒙古蒙牛乳业（集团）股份有限公司 | The method for producing sterile milk |
| CN111260099A (en) * | 2018-11-30 | 2020-06-09 | 内蒙古伊利实业集团股份有限公司 | Prediction method and prediction system for shelf life of liquid milk |
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| US5824494A (en) * | 1994-05-01 | 1998-10-20 | Sirotech Ltd. | Method for enumerating bacterial populations |
| CN101142939A (en) * | 2007-10-23 | 2008-03-19 | 内蒙古蒙牛乳业（集团）股份有限公司 | Middle temperature inactivation ultrahigh temperature instantaneous sterilizing milk and its preparation method |
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